The Journal of

Vol. 267, No. 18

June 25,1992

Biological Chemistry Copyright 0 1992 by the American Society for Biochemistry and Molecular Biology, Inc. 428 East Preston St., Baltimore, MD21202 U.S.A.

CONTENTS* 12462 On the mechanism of oxidation of cholesterol at C-7 in a lipoxygenasesystem. ErikLund, Ulf Diczfalusy,and Ingemar Bjorkhem

MINIREVIEW 12393 Regulation of inositol phospholipid-specific phospholipase C isozymes. Sue Goo Rhee and Kang Duk Choi

12468 Coupling of two endothelin receptor subtypes to differing signal transduction in transfected Chinese hamster ovary cells. Ichiro Aramori and Shigetada Nakanishi

COMMUNICATIONS 12397 Ligation activity of FLP recombinase. The strand liusing gationactivity of a site-specificrecombinase an activated DNA substrate. Guohau Pan andPaulD. Sadowski

12475 Complete amino acid sequence of the human a5(IV) collagen chain and identification of a single-base mutation in exon 23 converting glycine 521 in the collagenous domain to cysteine in an Alport syndrome paMichael Hertz, A n u Leinonen, and Karl tient. Jing Zhou, Jens Trygguason

12400 Chaperonins GroEL and GroES promote assembly of heterotetramers (ad2)of mammalianmitochondrial branched-chain a-keto acid decarboxylase in Escherichia coli. R. Max Wynn, James R.Dauie, Rody P. Cox, and David T. Chuang

12482 Identification of 5-oxo-15-hydroxy-6,8,11,13-eicosatetraenoic acid as a novel and potent humaneosinophil chemotactic eicosanoid. Uwe Schwenk, Eishin Morita, Regina Engel, and Jens-Michael Schroder

12404 Altered cellular interactions between endothelialcells and nonenzymaticallyglucosylated laminin/type IV collagen. Costas S. Haitoglou,Effie C. Tsilibary,Michael Brownlee, and Aristidis S. Charonis

12489 Purification and characterizationof the photosystem I complex fromthe filamentous cyanobacterium Anabaena variabilis ATCC 29413. Karin J. Nyhus, Masahiko Ikeuchi,YorinaoInoue,JohnWhitmarsh, andHimadriB. Pakrasi

12408 Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function. Jan Alsner, Jesper Q. Suejstrup,EigilKjeldsen,Boe S. S@rensen, and Ole Westergaard

12496 Chemical modification of the neutral aminoacid transport system L of Chinese hamster ovary cells with p chloromercuribenzene sulfonate. George S. Campbell, Jing Hua Yu, and Dale L. Oxender

12412 Post-transcriptional regulation of expression of plasminogen activator inhibitor type 1 mRNA by insulin and insulin-like growth factor1. Peter G. Fattal, David J. Schneider, Burton E.Sobel, and Joseph J. Billadello

12501 A synthetic sialic acid analogue isrecognized by influenza C virus as a receptor determinant but is resistant to the receptor-destroying enzyme. Georg Herder, Hans-Jiirgen Gross, Angela Imhof, Reinhard Brossmer, Glenda Milks, and James C. Paulson

12416 Activation of insulin receptor signaling by a single amino acid substitution in the transmembrane domain. Nicola Longo, Robert C. Shuster, Lorri D. Griffin, Sharon D. Langley, and Louis J. Elsas

12506 The primary structure of w-amino acid:pyruvate aminotransferase. Kazuo Yonaha, Masaichiro Nishie, and Shigeo Aibara

12420 The a2-macroglobulin receptorflow density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A. Maria Z. Kounnas, Randal E. Morris, Michael R. Thompson, David J . FitzGerald, Dudley K. Strickland, and Catherine B. Saelinger

12511 Phosphorylation of myosin light chain kinase by the multifunctional calmodulin-dependent protein kinase I1 in smooth muscle cells. Malu G. Tansey, R. A n n Word, HiroyoshiHidaka, Harold A . Singer,Charles M. Schworer, Kristine E. Kamm, and James T. Stull

12424 The A 2 0 zinc finger protein protects cells from tumor necrosis factor cytotoxicity.Anthony W.Opipari, Jr., Hong Ming Hu, Rachel Yabkowitz, and Vishva M. Dixit

12517 Selective inhibitorsof GTP synthesisimpede exocytotic insulin release from intact rat islets. Stewart A. Metz, Mary E. Rabaglia, and Thomas J. Pintar

12428 Overexpression of c-myc by amplification of negative promoter domain. S. Balakrishna Pai, Rekha E. Pai, and Randal N. Johnston

12528 Role of the antithrombin-binding pentasaccharide in heparin acceleration of antithrombin-proteinase reactions. Resolution of the antithrombin conformational change contribution to heparin rate enhancement. Steven T.Olson, Ingemar Bjark, Roberta Sheffer, Paul A. Craig, Joseph D. Shore, and Jean Choay

12432 Archaea containa novel diether phosphoglycolipid with a polar head group identical to the conserved core of eucaryal glycosyl phosphatidylinositol. Masateru Nishihara, Masami Utagawa, Hideo Akutsu, and Yosuke Koga 12436 Specific inhibition of hepatitis B viral gene expression in vitro by targeted antisense oligonucleotides. George Y. W u and Catherine H. Wu

12539 The cDNA for the type I iodothyronine 6"deiodinase encodes an enzyme manifesting both high K , and low K , activity. Evidence that rat liver and kidney contain a single enzyme which converts thyroxine to 3,5,3'-triiodothyronine. Jahangir Sharifi and Donald L. St. Germain

12440 Calcium and muscarinic agonist stimulation of type I adenylylcyclase in whole cells. Eui-Ju Choi,Scott T. Wong, Thomas R. Hinds, and Daniel R. Storm

ARTICLES 12443 Stimulation of sphingomyelin biosynthesis bybrefeldin A and sphingomyelin breakdown by okadaic acid treatment of rat hepatocytes. Grant M. Hatch and Dennis E. Vance

Ca" transient in 12545 Thapsigargin inhibits contraction and cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+pump. Mark S. Kirby, Yutaka Sagara, Shirley Gaa, Giuseppe Inesi, W . J . Lederer, and Terry E. Rogers

12552 Identification and expression of five mutations in the human acid sphingomyelinasegene causing types A and B Niemann-Pick disease. Molecular evidence for genetic heterogeneity in the neuronopathic and nonneuronopathic forms. Tsutomu Takahashi, Mariko Suchi, Robert J. Desnick, Goro Takada, and Edward H. Schuchman

12452 Substitution of the insulin receptor transmembrane domain withthe c-neulerbB2 transmembrane domain constitutively activates the insulin receptor kinase in vitro. Kazunori Yamada, Edison Goncalves, C. Ronald Kahn, and Steven E. Shoelson

* The CONTENTS arrangedby Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1992)J. Biol. Chem. 267,653-663,and reprints may be obtained from the editorial office.

... 111

V

12559 Stereoselective hydrolysis catalyzed by related 8-1,4-

12673

Role of carnitine and carnitine palmitoyltransferase as integral components of thepathwayformembrane phospholipid fatty acid turnover in intact human erythrocytes. Arduino Arduini, Giouanna Mancinelli, Giuseppe L. Radatti, Second0 Dottori, Francesco Molajoni, and Rona R. Ramsay

12682

Hemoglobin Thionville. An a-chain variant witha substitution of a glutamate for valine atNA-1 and having an acetylated methionine NH2 terminus. Corinne Vasseur, Yues Blowquit, Jean Kister, Danielle, Prome, Jeffrey S. Kauanaugh, Paul H. Rogers, Christlne Guzllemin, Arthur Arnone, Fridiric Galacteros, Claude Poyart, Jean Rosa, and Henri Wajcman

John Gebler, Neil R. glucanasesandfl-l,l-xylanases. Gilkes, Marc Claeyssens, Dauid B. Wilson,Pierre Beguin, Warren W. Wakarchuk, Douglas G. Kilburn, Robert C. Miller, Jr., R. Antony J . Warren, and Stephen G. Withers

12562 Evidence for the functionalinvolvement of protein ki-

nase C in the action of 1,25-dihydroxyvitamin D3 in bone. Johannes P. T. M. uan Leeuwen, Jan C. Birkenhager, Gert-Jan C. M. van den Bemd, Cok J . Buurman, Ada Staal, Martine P. Bos, and Huibert A. P. Pols

12570 Membrane topology of the ArsB protein, the membrane

subunit of an anion-translocating ATPase. Jianhw Wu, Louis S. Tisa, and Barry P. Rosen

12577 Golgi vacuolization and immunotoxin enhancement by

monensin and perhexiline depend on a serum protein. Implications for intracellular trafficking.Franz K. Jansen, Aline Jansen, Jean Marie Derocq, Dominique Carriere, Pierre Carayon, Francisco Veas, and Jean Pierre Jaffrezou

12583 Expression of cytochrome be in yeast and characteriza-

12692 Characterization of recombinanthumaninsulin-like

6 produced in growth factor binding proteins 4,5, and yeast. Michael C. Kiefer, Christoph Schmid, Margaretha Walduogel,Irene Schlapfer, Eua Futo, Frank R. Masiarz, Kim Green, Philip J. Barr, and Jurgen Zapf

12700 Characterization of prostaglandin (PC)-binding sites

expressed on human basophils. Evidence for a prostaglandin El, 12,and a D2 receptor. Irene Virgolini, Shuren Li, Christian Sillaber, Otto Majdic, Helmut Sinzinger, Klaus Lechner, Peter Bettelheim, and Peter Valent

tion of mutants of the membrane-anchoring domain. Guy VergBres and Lucy Waskell

12592 a-Isoenzyme of alcohol dehydrogenase from monkey

liver. Cloning, expression, mechanism, coenzyme, and substrate specificity. Dauid R. Light, Mark S. Dennis, Ian J . Forsythe, Chung-Chew Liu, Dauid W . Green, Darla Ann Kratzer, and Bryce V . Plapp

12600 Reversible stepwise mechanism involving a carbanion

intermediate in the elimination of ammonia from Lhistidine catalyzedby histidine ammonia-lyase. Takashi Furuta, Hidenori Takahashi,Hiromi Shibasaki, and Yasuji Kasuya

12606 Characterization of the inhibitionof intracellular Ca2+

transportATPases by thapsigargin. Yutaka Sagara, Francisco Fernandez-Belda, Leopoldode Meis, and Giuseppe Inesi

12614 Structureandligandbindingproperties

of leucine 29(B10) mutantsof human myoglobin. Shin-ichi Adachi, Nobw Sunohara, Koichiro Ishimori, and Isao Morishima

12709 Purification and characterization of a UDP-GalNAc:

polypeptide N-acetylgalactosaminyltransferasespecific for glycosylation of threonine residues. Yang Wang, John L. Abernethy, Allen E. Eckhardt, and Robert L. Hill

12717

Rapid, high yield purification and characterization of theK+-translocating Kdp-ATPase fromEscherichia coli. Annette Siebers, Ralf Kollmann, Gabriele Dirkes, and Karlheinz Altendorf

12722

Characterization of @-l2'I-endorphin cross-linked proteins in NGlOS-15 cell membranes. A 25-kilodalton protein with propertiesof 6-opioid-binding site. Jane L. KO, Nancy M.Lee, and Horace H. Loh

12728

Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants.Norio Ohushi, Hiroko Nashimoto, Hideo Ikeda, and Akira Tamura

2736

Adipsin and an endogenous pathway of complement from adipose cells. Lisa N. Choy, Barry S. Rosen, and Bruce M. Spiegelman

2742

Phosphorylation of the cystic fibrosis transmembrane conductance regulator. Marina R. Picciotto, Jonathan A. Cohn, Gloria Bertuzzi, Paul Greengard, and Angus C. Nairn

2753

Calcium is required forfolding of newly made subunits of the asialoglycoprotein receptorwithinthe endoplasmicreticulum. Harvey F. Lodish, Nancy Kong, and Lilian Wikstrom

12622 Escherichia coli ribonucleotide reductase. Radical sus-

ceptibility to hydroxyurea is dependent on the regulatory state of the enzyme. Margareta Karlsson, Margareta Sahlin, and Britt-Marie Sjoberg

12627 Caracemide, a site-specific irreversibleinhibitor

of protein R 1 of Escherichia coli ribonucleotide reductase. Ingrid Kjbller Larsen, Claw Cornett, Margareta Karlsson, Margareta Sahlin, and Britt-Marie Sjoberg

12632 0-Acetylated gangliosides in bovine buttermilk. Char-

acterization of 7-0-acetyl, 9-0-acetyl, and 7,9-di-0. Ren, J. Nee1 Scarsdale, Toshio Ariga, acetyl G D ~Shunlin Yuejin Zhang, Roger A. Klein, Rudolf Hartmann, Yasunori Kushi, Heinz Egge, and Robert K. Y u

12761 Purification and properties of the F sex factor TraD

protein, an inner membraneconjugal transfer protein. Mitradas M. Panicker and Edwin G. Minkley, Jr.

12639 Site-directed mutagenesis of serine 40 of rat tyrosine

hydroxylase. Effects of dopamine and CAMP-dependent phosphorylation on enzyme activity. S. Colette Daubner, Crystal Lauriano, John W.Haycock, and Paul F. Fitzpatrick

12767

Interaction of the -170 cyclic AMP response element with the adjacent CCAAT box in the human fibronectin gene promoter. And& F. Muro, Viuiana A. Bernath, and Alberta R. Kornblihtt

12775

A Pro/Ser substitution in nucleoside diphosphatekinase of Drosophila melanogaster (mutation Killer ofprune) affects stability but not catalytic efficiency of the en, hin Chaffotte, Bernadette Limzyme. Ioan h c u A bourg-Bouchon, and Michel Veron

12647 Post-transcriptional regulation of 3-hydroxy-3-meth-

ylglutaryl coenzyme A reductase by 24(S),25-oxidolanosterol. Sankhauaram R. Panini, Thomas A. Delate, and Michael Sinensky

12655 Preparation, purification, and determinationof the bi-

ological activities of 1 2 N terminus-truncated recombinant analogues of bovine placental lactogen. Arieh Gertler, Scott D. Hauser, EdnaSakal, Dorit Vashdi,Nick Staten, John J. Freeman, and Gwen G. Kriui

12660 Molecularcloning

of ratprostatetransglutaminase complementary DNA. The major androgen-regulated protein DP1 of rat dorsal prostate and coagulating gland. Kw-Chieh Ho, Valerie E. Quarmby, Frank S. French, and Elizabeth M. Wilson

12668 Small-angle x-ray scattering study

of metal ion-induced conformational changes in Serratia protease. Yoshio Katsuya,MamoruSato,Yukiteru Katsube, Yoshiki Matsuura, and Katsumi Tomoda

12782 The gene and cDNA for the human high affinity im-

munoglobulin E receptor 8 chain andexpression of the complete human receptor. Helmut Kiister, Li Zhang, Anna T . Brini, Don W. J.MacGloshan, and Jean-Pierre Kinet

12788 Enhanced insulin-induced mitogenesis and mitogen-ac-

tivatedproteinkinaseactivitiesinmutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. Akifumi Ando, Kaoru Momomura, Kazuyuki Tobe, Ritsuko Yamamoto-Honda, Hiroshi Sakura, Yoshikazu Tamori, Yasushi Kaburagi, Osamu Koshio, YasuoAkanuma,YoshioYazaki, Masato Kasuga, and Takashi Kadowaki

The Journal of Biological Chemistry (ISSN 0021-9258) is published three times per month by the American Society for Biochemistry and Molecular Biology. Inc., 428 East Preston Street, Baltimore, MD 21202, U.S.A. Volume 267 for 1992 United States, $670; other countries, $820. Single copy: United States, $23.00; other countries, $27.00. Special subscription rates are avadable to members, graduate students, and postdoctoral fellows for personal use only. Quahfying forms areavailable from the ASBMB Office. 9650 Rockville Pike, Bethesda, MD 20814. Secqnd class ostage paid a t Baltimore, MD 21202, U.S.A. and at additional mailing offices. Send address changes to The Journal of Biological Chemistry a t 428 East Preston Street, Baltimore, 21202. U.S.A.

ME

vi 12797 Cloning and sequencing of a cDNA encoding 2,3-bisphosphoglycerate-independentphosphoglycerate mutase from maize. Possible relationship to the alkaline phosphatase family. Xauier Graria, Luis de Lecea, M. Raafat

El-Maghrabi, Jesus M. Ureria, Carme Caellas, Jose Carreras, Pere Puigdomenech, Simon J. Pilkis, and Fernando Climent 12804 Depletion of poly(ADP-ribose) polymerase by antisense RNA expression results ina delay inDNA strand break rejoining. Ruchuang Ding, Yues Pommier,Veronica H. Kang,

and Mark Smulson

12813 Dissection of the ,9 subunit in the Escherichia coli RNA polymerase into domainsby proteolytic cleavage. Kon-

stantin Seuerinou, Arkady Mustaeu, Mikhail Kashleu, Sergei Borukhou, Vadim Nikiforou, and Alex Goldfarb 12820 Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. William Kastern, Ulf Sjobring, and Lars Bjorck 12826 Tumor necrosis factor-a activates nuclear factor KB and induces manganoussuperoxide dismutase and phosphodiesterase mRNA in human papillary thyroid carcinoma cells. Xuan-Ping Pang, Nathan S. Ross, Minha Park,

Guy J . F. Juillard,ThomasM.Stanley, Hershman

and JeromeM.

12831 Induction of myogenic differentiation by an expression vector encoding the DNA methyltransferase cDNA sequence in the antisense orientation. MosheSzyf,Julie

Rouleau, Johanne Theberge, and Vera Bozouic

12837 Reversible binding of platelet-derived growth factorAA, -AB, and -BB isoforms to a similar site on the “slow” and “fast” conformations of az-macroglobulin.

James C. Bonner, Audrey L. Goodell, Joseph A. Lasky, and Maureane R. Hoffman

12845 Chicken vinculin and meta-vinculin are derived from a single geneby alternative splicingof a 207-base pair exon unique to meta-vinculin.Barry J. Byrne, Yolanda J.

Kaczorowski, Michelle D. Coutu, and Susan W. Craig

12936 Heat shock protein 27 and aB-crystallin can form a complex, which dissociates by heat shock. Alt Zantema,

Matty Verlaan-De Vries, Diedka Maasdam, Sandra Bol, and Alex uan der Eb

12942 Sequence and specificity of a soluble lactose-binding lectin from Xenopw laeuis skin.Philippe Marschal, Jorg

Herrmann, Hakon Leffler, Samuel H. Barondes, and Douglas N. W. Cooper

12950 A deletion in the ornithine aminotransferase gene in gyrate atrophy. Yasushi Akaki, Yoshihiro Hotta, Yukihiko

Mashima, Akira Murakami, Nancy G. Kennaway, Richard G. Weleber, and George Inana 12955 A panel of monoclonal antibodies for the type I insulinlike growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity. Maria A. Soos,

Catherine E. Field, Reiner Lammers, Axel Ullrich, Bei Zhang, Richard A. Roth, Asser S. Andersen, Thomas Kjeldsen, and Kenneth Siddle

12964 Release and recycling of eukaryotic initiation factor2 in the formation of an 80 S ribosomal polypeptidechain initiation complex. Amitabha Chakrabarti and Umadas

Maitra

12973 Structural characterizationof a novel cholinergic neuron-specific ganglioside in bovine brain. Yoshio Hirabay-

ashi, Toru Nakao, Fumitoshi Irie, Victor P. Whittaker, Kazuo Kon, and Susumu Ando

12979 Cardiac expressions of a- and @-myosinheavy chains and sarcomeric a-actins are regulated through transcriptional mechanisms. Results from nuclear run-on assays in isolated rat cardiacnuclei. Kenneth R. Boheler,

Catherine Chassagne, Xauier Martin, Claudine Wisnewsky, and Ketty Schwartz

12986 Four conserved cysteine residues are required for the DNA binding activity of nuclear factor I. Anton Nouak,

Neerja Goyal, and Richard M. Gronostajski

12851 Efficient plasma membrane expression of a functional platelet glycoprotein Ib-IX complex requires the presence of its three subunits. Josi A. Ldpez, Betty Leung,

12991 Protein phosphatase inhibitors induce thesustained expression of the Egr-1 gene and the hyperphosphorylation of its geneproduct. Xinmin Cao, Ratha Mahendran,

12860 Oligomycin sensitivity-conferring protein (OSCP) of mitochondrial ATP synthase. The carboxyl-terminal region of OSCP is essential for the reconstitution of oligomycin-sensitive H+-ATPase.Saroj Joshi, A. A.Jawed,

12998 Pro-opiomelanocortin synthesized by corticotrophs bears asparagine-linked oligosaccharides terminating Timothy P. with S04-4GalNAc,91,4GlcNAc,91,2Mana.

Clifford C. Reynolds, Chester Q. Li, and Joan E. B. Fox

and Leslie C. Gibbs 12868 Somatostatin gene transcription regulated by a bipartite pancreatic islet D-cell-specific enhancer coupled synergetically to a CAMP response element. Mario Val-

lejo, Christopher P. Miller, and Joel F. Habener element acti12876 Somatostatingeneupstreamenhancer vated by a protein complex consisting of CREB, Isl-llike, and a-CBF-like transcription factors. Mario Vallejo,

Lisa Penchuk, and Joel F. Habener 12885 Protein targeting across the three membranes of the Euglena chloroplastenvelope. ShashidharaL. S., Saw Hoon Lim, Jamie B. Shackleton, Colin Robinson, and Alison

G. Smith 12892 Expression of four protein kinase C isoforms in rat fibroblasts. Distinct subcellular distribution and regulation by calcium and phorbol esters. Christoph Borner,

Sarah Nichols G d a g n o , Doriano Fabbro,and Weinstein

I. Bernard

12900 Expression of four protein kinase C isoforms in rat fibroblasts. Differential alterations in rm-, src-, and fos-transformed cells. Christoph Borner, Sarah Nichols

Guudagno, WendyW.-L. Hsiao, Doriano Fabbro, Maureen Barr, and I. Bernard Weinstein 12911

PI- and f13-subunits can associate with presynthesized

a-subunits of Xenopus oocyte Na,K-ATPase. Ulrich Ackermann and Kathi Geering 12916 Irradiation of the bovine mitochondrial F1-ATPase previously inactivated with 5’-p-fluorosulfonylbenzoyl-8a~ido-[~H]adenosine cross-links His-,9427 to Tyr-8345 Garrod, within the same ,9 subunit. ShaoqiuZhuo,Siu Pamela Miller, and William S. Allison 12928 Molecular cloning and analysis of an eIF-4A-related rat liver nuclear protein. SaraswathyNair, Rama Dey,

Janet P. Sanford, and Darrell Doyle

Graeme R. Guy, and Y. H. Tan

Skelton, Swatantar Kumar, Peter L. Smith, Mary C. Beranek, and Jacques U. Baenziger

13007 The human gene for renin-binding protein. Smri Taka-

hashi, Hiroyasu Inoue, and Yoshihiro Miyake 13014 Thyroid hormone receptor mutants that cause resistance to thyroid hormone. Evidence for receptor competition for DNA sequences in target genes. Takashi

Nagaya, Laird D. Madison, and J . Larry Jameson

13020 Expression of an anchored urokinase in the endoapical thelial cell membrane.Preservation of enzymatic activity and enhancement of cell surface plasminogen activation. Sung W . Lee, Mark L. Kahn, and Dauid A. Dichek 13028 Evidence that mutations in a loop region of the asubunit inhibit the transition from anopen to a closed conformationinthetryptophansynthase bienzyme complex. Peter S. Brzouic’, Yoshihiro Sawa, C. Craig Hyde,

Edith W. Miles, and Michael F. Dunn

13039 The amino terminusof ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport.Rich-

ard A. Kahn, Paul Randazzo, Tito Serafini,Ofra Weiss, Cherrie Rulka, Jenny Clark, Mylene Amherdt,Peter Roller, Lelio Orci, and James E. Rothman

13047 Evidence for ADP-ribosylation factor (ARF) as a regulator of in uitroendosome-endosome fusion. James M.

Lenhard, Richard A. Kahn, and Philip D. Stahl 13053 ADP-ribosylation factor is required for vesicular trafcisficking between the endoplasmic reticulum and the Golgi compartment. William E. Balch, Richard A. Kahn,

and Ruth Schwaninger 13062 A common sequence motif, -E-G-Y-A-T-A-, identified within the primasedomains of plasmid-encoded I- and P-type DNA primases and the a protein of the Escherichia coli satellite phage P4. Bettlna Strack, Monika Lessl,

Richard Calendar, and Erich Lanka

vii

ADDITIONS AND CORRECTIONS

13073 The epithelin precursor encodes two proteins with op-

posing activities on epithelial cell growth. GregoryD. Plowman, Jane11 M. Green, Michael G. Neubauer, Sharon D. Buckley, Vicki L.McDonald, George J. Todaro, and Mohammed Shoyab

13079

An actin footprint on villin. Single site substitutions in a cluster of basic residues inhibit the actin severing but not capping activityof villin. Monika V. de Arruda, Hasan Bazari, Malgorzata Wallek, and Paul Matsudaira

13086 Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli. Eric D. Brown and Janet M. Wood 13093

A 13-amino acid N-terminal domain of a basic prolinerich protein is necessary for storage in secretory granules and facilitates exit from the endoplasmic reticulum. Anna M. Castle, Lisa E. Stahl, and J. David Castle

13113 The signal for Golgi retention of bovine @1,4-galacto-

syltransferase is in the transmembrane domain. Vol. 267(1992)4084-4096. Rohan D. Teasdale, Giacomo D’Agostaro, and Paul A. Gleeson

13114

13114 Effect of anoxiaonintracellularATP,

Na+i, Ca*+i, M8+i, and cytotoxicity in rat hepatocytes. Vol. 267 (1992) 6654-6663. Antonio Gasbarrini, AndreB. Borle, Hassan Farghalt, Connre Bender,Antonio Francauillu, and David Van Thiel

13114 Fructose protects rat hepatocytes from anoxic injury.

Vol. 267 (1992) 7545-7552. Antonio Gasbarrini, Andre B. Borle, Hassan Farghali, Antonio Francauilla, and Dauid Van Theil

13101 Internalization of the human insulin receptor. The in-

sulin-independent pathway. Jean-Pierre Paccaud, Kenneth Siddle, and Jean-Louis Carpentier

13107 Platelet-derived growth factor inducesc-fms and scav-

enger receptor genes in vascular smooth muscle cells. Toshimori Inaba, Takanari Gotoda, Hitoshi S h i m n o , Masako Shimuda,Kenji Harada, Koichi Kozaki, Yoshiro Watanabe, Eitetsu Hoh, Kazuo Motoyoshi, Yoshio Yazaki, and Nobuhiro Yamada

Site-directed mutagenesis in rat liver 6-phosphofructo2-kinase mutation at the fructose 6-phosphate binding site affectsphosphateactivation. Vol. 267(1992) 4386-4393. Lin Li,KaiLin,Irwin J. Kurlund,John J . Correia, and Simon J . Pilkis

13114

xiii

Requirements for primer synthesis by bacteriophage T7 63-kDa gene4 protein: roles of template sequence and T7 56-kDa gene4 protein. Vol. 266 (1991)2324023250. Lynn V. Mendelman and Charles C. Richardson Cumulative author index. Numbers 1 through 18.

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The Journal of Biological Chemistry

Vol. 267,No. 18

June 25, 1992

CONTENTS Arrange!d by Subject Categories MINIREVIEW

12511 Phosphorylation of myosin light chain kinase by the multifunctional calmodulin-dependent protein kinase I1 in smooth muscle cells. Malu G. Tansey, R. Ann Word, HiroyoshiHidaka, Harold A. Singer,Charles M. Schworer, Kristine E. Kamm, and James T. Stull

12393 Regulation of inositol phospholipid-specific phospholipase C isozymes. Sue Goo Rhee and Kang Duk Choi

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

12517 Selective inhibitorsof GTP synthesisimpede exocytotic insulin release from intact rat islets. Stewart A. Metz, Mary E. Rabaglia, and Thomas J. Pintar

-12432 Archaea contain a novel diether phosphoglycolipid with a polar head group identical to the conserved core of eucaryal glycosyl phosphatidylinositol. Masateru Nishihara, Masami Utagawa, Hideo Akutsu, and Yosuke Koga

12545 Thapsigargin inhibits contraction andCa2+transient in cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+ pump.Mark S. Kirby, Yutaka Sagara, Shirley Gaa, Giuseppe Inesi, W. J . Lederer, and Terry B. Rogers

12462 On the mechanism of oxidation of cholesterol at C-7 in a lipoxygenasesystem. ErikLund, Vlf Diczfalusy,and Ingemar Bjorkhem

12562 Evidence for the functional involvement of protein kinase C in the action of 1,25-dihydroxyvitamin D3 in bone. Johannes P. T. M . uan Leeuwen, J a n C. Birkenhager, Gert-Jan C. M. uan den Bemd, Cok J. Buurman, Ada Staal, Martine P. Bos, and Huibert A. P. Pols

12482 Identification of 5-oxo-15-hydroxy-6,8,11,13-eicosatetraenoic acidas a novel and potent humaneosinophil chemotactic eicosanoid. Uwe Schwenk, Eishin Morita, Regina Engel, and Jens-Michael Schroder

12577 Golgi vacuolization and immunotoxin enhancement by monensin and perhexiline depend on a serum protein. Implications for intracellular trafficking.Franz K. Jansen, Aline Jansen, Jean Marie Derocq, Dominique Carriere, Pierre Carayon, Francisco Veas, and Jean Pierre Jaffrezou

12632 0-Acetylated gangliosides in bovine buttermilk. Characterization of 7-0-acetyl, 9-0-acetyl, and 7,9-di-0acetyl Gn3. Shunlin Ren, J. Nee1 Scarsdale,ToshioAriga, Yuejin Zhang, Roger A . Klein, Rudolf Hartmann, Yasunori Kushi, Heinz Egge, and Robert K . Y u

12647 Post-transcriptional regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by 24(S),25-oxidolanosterol. Sankhauaram R. Panini, Thomas A. Delate, and Michael Sinensky

12709 Purification and characterization of a UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase specific for glycosylation of threonine residues. Yang Wang, John L. Abernethy, Allen E. Eckhardt, and RobertL. Hill 12973 Structural characterization of a novel cholinergic neuron-specific ganglioside in bovine brain. Yoshio Hirabayashi, Toru Nakao, Fumitoshi Irie, Victor P. Whittaker, Kazuo Kon, and Susumu Ando

12673 Role of carnitine and carnitine palmitoyltransferase as integral components of thepathwayformembrane phospholipid fatty acid turnover in intact human erythrocytes. Arduino Arduini, Giouanna Mancinelli, Giuseppe L. Radatti,SecondoDottori,FrancescoMolajoni,andRonaR. Ramsay

12998 Pro-opiomelanocortin synthesized by corticotrophs bears asparagine-linked oligosaccharides terminating with SO4-4GalNAcj31,4G1cNAcj31,2Mana.TimothyP. Skelton, Swatantar Kumar, Peter L. Smith, Mary C. Beranek, and Jacques U. Baenziger

12692 Characterization of recombinanthumaninsulin-like growth factor binding proteins 5, 4, and 6 produced in yeast. MichaelC.Kiefer,ChristophSchmid,Margaretha Walduogel, Irene Schliipfer, Eua Futo, Frank R. Masiarz, Kim Green, Philip J. Barr, and Jiirgen Zapf 12700 Characterization of prostaglandin (PG)-binding sites expressed on human basophils. Evidence for a prostaglandin E,, 12,.and a DZreceptor. Irene Virgolini, Shuren Li, Christian Scllaber, Otto Majdic, Helmut Sinzinger, Klaus Lechner, Peter Bettelheim, and Peter Valent

CELL BIOLOGY AND METABOLISM -12404 Altered cellular interactions between endothelial cells and nonenzymaticallyglucosylated lamininltype IV collagen. Costas S.Haitoglou,EffieC.Tsilibary,Michael Brownlee, and Aristidis S. Charonis ‘12416

12753 Calcium is required for folding of newly madesubunits of the asialoglycoprotein receptorwithinthe endoplasmicreticulum. Haruey F. Lodish,NancyKong,and Lilian Wikstrom

Activation of insulinreceptorsignaling by a single amino acid substitution in the transmembranedomain. Nicola Longo, Robert C. Shuster, Lorri D. Griffin, Sharon D. Langley, and Louis J . Elsas

12788 Enhanced insulin-induced mitogenesis and mitogen-activatedproteinkinaseactivitiesinmutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. A k ifumi Ando, Kaoru Momomura, Kazuyuki Tobe, Ritsuko Yamamoto-Honda, Hiroshi Sakura, Yoshikazu Tamori, Yasushi Kaburagi,OsamuKoshio,YasuoAkanuma,YoshioYazaki, Masato Kasuga, and Takashi Kadowaki

-12420 The a,-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A. Maria 2. Kounnas, Randal E. Morris, Michael R. Thompson, David J . FitzGerald, Dudley K. Strickland, and Catharine B. Saelinger -12424

The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. Anthony W.Opipari, Jr., Hong Ming Hu, Rachel Yabkowitz, and Vishua M. Dixit

12826 Tumor necrosis factor-a activates nuclear factor KB and induces manganous superoxide dismutase phosphoand diesterase mRNA in human papillary thyroid carcinoma cells. Xuan-Ping Pang, Nathan S. Ross, Minha Park, Guy J. F. Juillard, Thomas M. Stanley, and Jerome M. Hershman

012440 Calcium and muscarinic agonist stimulation of type I adenylylcyclase in whole cells. E u i - J uC h i ,S c o t t T. Wong, Thomas R. Hinds, andDaniel R. Storm 12443 Stimulation of sphingomyelin biosynthesis by brefeldin A and sphingomyelin breakdown by okadaic acid treatment of rat hepatocytes. Grant M. Hatch and Dennis E. Vance

12831 Induction of myogenic differentiation by an expression vector encoding the DNA methyltransferase cDNA sequence in the antisense orientation. Moshe Szyj, Julie Rouleau, Johanne Theberge, and Vera Bozouic

12452 Substitution of the insulin receptor transmembranedomain withthe c-neulerbB2 transmembrane domain constitutively activates the insulin receptor kinase in vitro. Kazunori Yamada, Edison Goncalues, C. Ronald Kahn, and Steven E. Shoelson

12837 Reversible binding of platelet-derived growth factorAA, -AB, and -BB isoforms to a similar site on the “slow” and “fast” conformations of a,-macroglobulin. James C. Bonner, Audrey L. Goodell, Joseph A. Lasky, and Maureane R. Hoffman

12468 Coupling of two endothelin receptor subtypes to differing signal transduction in transfected Chinese hamster ovary cells. Ichiro Aramori and Shigetada Nakankhi

12911

Indicates a Communication

viii

B1-and fl3-subunits can

associate with presynthesized a-subunits of Xenopus oocyte Na,K-ATPase. Ulrich Ackermann and KathiGeering

ix 12936 Heat shock protein 27 and aB-crystallin can form a complex, which dissociates by heat shock. Alt Zantema, Matty Verlaan-De Vries, Diedka Maasdam, Sandra Bol, and Alex van der Eb

12592 a-Isoenzyme of alcohol dehydrogenasefrom monkey liver. Cloning, expression, mechanism, coenzyme, and substrate specificity. Dauid R. Light, Mark S. Dennis, Ian J. Forsythe, Chung-Cheng Liu, Dauid W . Green, Darla A n n Kratzer, and Bryce V . Plapp

I insulin12955 A panel of monoclonal antibodies for the type like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity. Maria A.Soos, Catherine E. Field, Reiner Lummers, Axel Ullrich, Bei Zhang, Richard A. Roth, Asser S. Andersen, Thomas Kjeldsen, and Kenneth Siddle

12600 Reversible stepwise mechanism involving a carbanion intermediate in the elimination of ammonia from Lhistidine catalyzedby histidine ammonia-lyase. Takashi Furuta,HidenoriTakahashi,HiromiShibasaki,andYasuji Kasuya

12991 Proteinphosphataseinhibitorsinducethesustained expression of the Egr-1 gene and the hyperphosphorylation of its geneproduct. Xinmin Cao, Ratha Mahendran, Graeme R. Guy, and Y. H . T a n

12606 Characterization of the inhibition of intracellular Caz+ transportATPases by thapsigargin. Yutaka Sagara, Francisco Fernandez-Belda, Leopoldo de Meis, and Giuseppe Inesi

13039 The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport. Richurd A. Kahn, Paul Randazzo, Tito Serafini, Ofra Weiss, Cherrie Rulka, Jenny Clark, Mylene Amherdt, Peter Roller, LelioOrci, and James E. Rothman

12622 Escherichia coli ribonucleotide reductase. Radical susceptibility to hydroxyurea is dependenton the regulatory state of the enzyme. Margareta Karlsson, Margareta Sahlin, and Britt-Marie Sjoberg

13047 Evidence for ADP-ribosylation factor (ARF) as a regulator of in vitro endosome-endosome fusion. James M. Lenhard, Richard A. Kahn, and Philip D. Stahl 13053 ADP-ribosylation factor is required for vesicular trafficking between the endoplasmic reticulum and the cisGolgi compartment. William E. Balch, Richard A. Kahn, and Ruth Schwaninger 13079 An actin footprint on villin. Single site substitutions in a cluster of basic residues inhibit the actin severing but not capping activityof villin. Monika V. de Arruda, Hasan Bazari, Malgorzata Wallek, and Paul Matsudaira 13093 A 13-amino acid N-terminal domain of a basic prolinerich protein is necessary for storage in secretory granules and facilitates exit from the endoplasmic reticulum. Anna M. Castle, Lisa E. Stahl, and J. David Castle 13101 Internalization of the human insulin receptor. The insulin-independent pathway. Jean-PierrePaccaud,Kenneth Siddle, and Jean-Louis Carpentier 13107 Platelet-derived growth factor induces c-fms and scavenger receptor genes in vascular smooth muscle cells. Toshimori Inuba, Takanari Gotoda, Hitoshi Shimano, Masako Shimada,Kenji Harada,KoichiKozaki,YoshiroWatanabe, Eitetsu Hoh, Kazuo Motoyoshi, Yoshio Yazaki, and Nobuhiro Yamada 13113 The signal for Golgi retention of bovine 81,4-galactosyltransferase is in the transmembrane domain. Vol. 267(1992) 4084-4096. RohanD.Teasdale,Giacomo D’Agostaro, and Paul A. Gleeson 13114 Effect of anoxia on intracellularATP, Na+i, Caz+i, MgZ+;,and cytotoxicity in rat hepatocytes. Vol. 267 (1992) 6654-6663. AntonioGasbarrini,AndreB.Borle, HassanFarghali,ConnieBender,AntonioFrancavilln,and David Van Thiel 13114 Fructose protects rat hepatocytes from anoxic injury. Vol. 267 (1992) 7545-7552. Antonio Gasbarrini, Andre B . Borle, Hassan Farghali, Antonio Francavilln, and Davzd V a n Theil

ENZYMOLOGY 12501 A synthetic sialic acid analogue is recognized by influenza C virus as a receptor determinant but is resistant to the receptor-destroying enzyme. Georg Herder, Hans-Jiirgen Gross, Angela Imhof, Reinhard Brossmer, Glenda Milks, and James C. Paulson 12539 The cDNA for the type I iodothyronine 5”deiodinase encodes an enzyme manifesting both high K , and low K , activity. Evidence that rat liver and kidney contain a single enzyme which converts thyroxine to 3,5,3’-triiodothyronine.Jahangir Sharifi and Donald L. St. Germain 12559 Stereoselective hydrolysis catalyzed by related &1,4glucanasesand&1,4-xylanases. J o h n Gebler, NeilR. Gilkes, Marc Clneyssens, DavidB. Wilson, Pierre Beguin, Warren W . Wakarchuk, D o u g h G. Kilburn, Robert C. Miller, Jr., R. Antony J. Warren, and Stephen G. Withers

Indicates a Communication

12627 Caracemide, a site-specific irreversibleinhibitor of protein R 1 of Escherichia coli ribonucleotide reductase. Ingrid Kjdler Larsen, Claw Cornett, Margareta Karlsson, Margareta Sahhn, and Britt-Marie Sjoberg 12775 A ProISer substitution innucleoside diphosphate kinase of Drosophila melanogaster(mutation Killer of prune) affects stability but not catalytic efficiency of the enzyme. Ioan Lascu, Alain Chaffotte, Bernadette Limbourg-Bouchon, and Michel Veron 13028 Evidence thatmutationsin a loop region of the (Isubunit inhibit the transition from anopen to a closed conformationinthetryptophansynthase bienzyme complex. Peter S. Brzouic’, Yoshihiro Sawa, C. Craig Hyde, Edith W. Miles, and Michael F. Dunn

MEMBRANES AND BIOENERGETICS 12489 Purification and characterization of the photosystem I complex from the filamentous cyanobacterium Anabaena variabilis ATCC 29413. Karin J. Nyhus, Masahiko Ikeuchi,YorinaoInoue,JohnWhitmarsh,andHimadri B. Pakrasi 12496 Chemical modification of the neutral amino acid transport system L of Chinese hamster ovary cells with p chloromercuribenzene sulfonate.George S. Campbell, Jing Hua Yu, and Dale L. Oxender 12570 Membrane topology of the ArsB protein, the membrane subunit of an anion-translocatingATPase. Jianhua Wu, Louis S. Tisa, and Barry P. Rosen 12717 Rapid, high yield purification and characterization of theK+-translocating Kdp-ATPase from Escherichia

coli. Annette Siebers, Ralf Kollmann, Gabriele Dirkes,and Karlheinz Altendorf

12860 Oligomycin sensitivity-conferringprotein (OSCP) of mitochondrial ATP synthase. The carboxyl-terminal region of OSCP is essential for the reconstitution of oligomycin-sensitive H+-ATPase.Saroj Joshi, A. A. Jaued, and Leslie C. Gibbs 12885 Protein targeting across the three membranes of the Euglena chloroplast envelope. ShashidharaL. S., Saw Hoon Lim, Jamie B. Shackleton, Colin Robinson, and Alison G. Smith 12916 Irradiation of the bovine mitochondrial F,-ATPase previously inactivated with 5’-p-fluorosulfonylbenzoyl-8a~ido-[~H]adenosine cross-links His-j3427 to Tyr-8345 within the same 8 subunit. Shaoqiu Zhuo, Siu Garrod, Pamela Miller, and William S. Allison

NUCLEIC ACIDS, PROTEINSYNTHESIS, MOLECULAR GENETICS

AND

‘12397

Ligation activity of FLP recombinase. The strand ligationactivity of a site-specificrecombinaseusing an activated DNA substrate. Guohau Pan andPaulD. Sadowski

-12400

Chaperonins GroEL and GroES promote assembly of heterotetramers (a2&) of mammalianmitochondrial branched-chain a-keto acid decarboxylase in Escherichia coli.R. Max Wynn, James R.Dauie, Rody P. Cox, and David T. Chuang

X

012408 Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function. Jan Alsner, Jesper Q. Svejstrup, Eigil Kjeldsen, Boe S. Sfirensen, and Ole Westergaard

12892 Expression of four protein kinase C isoforms in rat fibroblasts. Distinct subcellular distribution and regulation by calcium and phorbol esters. Christoph Borner, Sarah Nichols G d a g n o , Doriano Fabbro,and I. Bernard Weinstein

012412 Post-transcriptional regulation of expression of plas-

12900 Expression of four protein kinase C isoforms in rat fibroblasts. Differential alterations in r w - , src-, and fos-transformed cells. Christoph Borner, Sarah Nichols G d a g n o , WendyW.-L. Hsiao, Doriano Fabbro, Maureen Barr, and I. Bernard Weinstein

minogen activator inhibitor type 1 mRNA by insulin and insulin-like growth factor 1.Peter G. Fattal, David J. Schneider, Burton E. Sobel, and Joseph J. Billadella

*12428 Overexpression of c-myc by amplification of negative promoter domain. S. Balnkrishna Pai, Rekha B. Pai, and Randal N. Johnston 012436 Specific inhibition of hepatitis B viral gene expression in vitro by targeted antisense oligonucleotides. George Y. W u and Catherine H. W u 12475 Complete aminoacid sequence of the human a5(IV) collagen chain and identification of a single-base mutation in exon2 3 converting glycine 521 in the collagenous domain to cysteine in an Alport syndrome paMichael Hertz, Anu Leinonen, and Karl tient. Jing Zhou, Jens Tryggvason 12552 Identification and expression of five mutations in the human acidsphingomyelinase gene causingtypes A and B Niemann-Pick disease. Molecular evidence for genetic heterogeneity in the neuronopathic and nonneuronopathic forms. Tsutomu Takahmhi, Mariko Suchi, Robert J . Desnick, Goro Takada, and Edward H. Schuchman 12660 Molecularcloning of ratprostatetransglutaminase complementary DNA. The major androgen-regulated protein DP1 of rat dorsal prostate and coagulating gland. Kuo-Chieh Ho, Valerie E. Quurmby, Frank S. French, and Elizabeth M. Wilson 12728 Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA Norio Ohashi, homologues from four antigenic variants. Hiroko Nashimoto, Hideo Ikeda, and Akira Tamura 12736 Adipsin and an endogenous pathway of complement from adipose cells. Lisa N. Choy, Barry S. Rosen, and Bruce M. Spiegelman 12761 Purification and properties of the F sex factor TraD protein, an inner membrane conjugal transfer protein. Mitradas M. Panicker and Edwin G. Minkley, Jr. 12767 Interaction of the -170 cyclic AMP response element with the adjacent CCAAT box in the human fibronectin gene promoter. And& F. Muro, Viuiana A. Bernath, and Albert0 R. Kornblihtt 12782 The gene and cDNA for the human high affinity immunoglobulin E receptor fl chain and expressionof the complete human receptor.Helmut Kiister, Li Zhang, Anna T. Brini, Don W. J.MacGlashan, and Jean-Pierre Kinet 12797 Cloning and sequencing of a cDNA encoding 2,3-bisphosphoglycerate-independent phosphoglycerate mutase from maize. Possible relationship to the alkaline phosphatase family. Xavier Graiiu, Luis de Lecea, M. Raufat El-Maghrabi, Jeslis M. Ureiin, Carme Caellas, Jose Cameras, Pere Puigdomenech, Simon J . Pilkis, and Fernando Clrment 12804 Depletion of poly(ADP-ribose) polymerase byantisense RNA expression results ina delay inDNA strand break rejoining. Ruchuang Ding, Yves Pommier,Veronica H. Kang, and Mark Smulson 12845 Chicken vinculin and meta-vinculin are derived from a single geneby alternative splicingof a 207-base pair exon unique to meta-vinculin. Barry J . Byrne, Yolnnda J. Kaczorowski, Michelle D. Coutu, and Susan W. Craig 12868 Somatostatin gene transcription regulated by a bipartite pancreatic islet D-cell-specific enhancer coupled synergetically to a CAMP response element. Marlo Vallejo, Christopher P. Miller, and Joel F. Habener 12876 Somatostatingeneupstreamenhancerelementactivated by a protein complex consisting of CREB, Isl-llike, and a-CBF-like transcription factors. Mario Vallejo, Lisa Penchuk. and Joel F. Habener

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12928 Molecular cloning and analysis of an eIF-4A-related rat liver nuclear protein. Saraswathy Nair, Rama Dey, Janet P. Sanford, and Darrell Doyle 12950 A deletion in the ornithine aminotransferase gene in gyrate atrophy. Yasushi Akaki, Yoshihiro Hotta, Yukihiko Mashima, Akira Murakami, Nancy G. Kennaway, Richard G. Weleber, and George Inana 12964 Release and recyclingof eukaryotic initiation factor2 in the formation of a n 80 S ribosomal polypeptidechain initiation complex. Amitabha Chakrabarti and Umadas Maitra 12979 Cardiac expressions of a- and &myosin heavy chains and sarcomeric a-actins are regulated through transcriptional mechanisms. Results from nuclear run-on assays inisolated rat cardiacnuclei. Kenneth R. Boheler, Catherine C h s a g n e , XavierMartin, Claudine Wisnewsky, and Ketty Schwartz 12986 Four conserved cysteine residues are required for the DNA binding activity of nuclear factor I. Anton Novak, Neerja Goyal, and Richard M. Gronostajski

Saori Taka13007 The human gene for renin-binding protein. hashi, Hiroyasu Inoue, and Yoshihiro Miyake 13014 Thyroid hormone receptor mutants that cause resistance to thyroid hormone. Evidence for receptor competition for DNA sequences in target genes. Takashi Nagaya, Laird D. Madison, and J. Larry Jameson apical 13020 Expression of an anchored urokinase in the endothelial cell membrane.Preservation of enzymatic activity and enhancementof cell surface plasminogen activation. Sung W . Lee, Mark L. Kahn, and David A. Dichek 13062 A common sequence motif, -E-G-Y-A-T-A-, identified within the primasedomains of plasmid-encoded I- and P-type DNA primases and the a protein of the Escherichia coli satellite phage P4. Bettina Strack,Monika Lessl, Richard Calendar, and Erich Lunka 13114 Requirements for primer synthesis by bacteriophage T7 63-kDa gene4 protein: roles of template sequence and T7 66-kDa gene 4 protein. Vol. 266 (1991) 2324023250. Lynn V. Mendelman and Charles C. Richardson

PROTEINCHEMISTRY AND STRUCTURE 12506 The primary structureof w-amino acid:pyruvate aminotransferase. Kazuo Yonaha, Masaichiro Nishie, and Shigeo Aibara 12528 Role of the antithrombin-binding pentasaccharide in heparin acceleration of antithrombin-proteinase reactions. Resolution of the antithrombin conformational change contribution to heparin rate enhancement. Steuen T. Olson, Ingemar Bjork, Roberta S h f f e r , Paul A. Craig, Joseph D. Shore, and Jean Choay 12583 Expression of cytochrome A in yeast and characterization of mutants of the membrane-anchoring domain. Guy Vergeres and Lucy Waskell 12614 Structureandligandbindingproperties of leucine 29(B10) mutantsof human myoglobin. Shin-ichi Adachi, Nobuo Sunohara, Koichiro Ishimori, and I s m Morishima 12639 Site-directed mutagenesis of serine 40 of rat tyrosine hydroxylase. Effects of dopamine and CAMP-dependent phosphorylation on enzyme activity. S. Colette Daubner, Crystal Lauriano, John W. Haycock, and Paul F. Fitzpatrick 12655 Preparation, purification, and determinationof the biological activities of 1 2 N terminus-truncated recombinant analogues of bovine placental lactogen. Arieh Gertler, Scott D. Hauser, Edna Sakal, Dorit Vashdi,Nick Staten, John J.Freeman, and Gwen G. Krwc

xi 12668 Small-anglex-rayscatteringstudy duced conformational changes in Yoshio Katsuya,MamoruSato,Yukiteru Matsuura, and Katsumi Tomoda

of metal ion-inSerratia protease. Katsube, Yoshiki

12682 Hemoglobin Thionville. An a-chain variant with a substitution of a glutamate for valine atNA-1 and having an acetylated methionine NH2 terminus. Corinne Vasseur, Yues Blouquit, Jean Kister, Danielle Prome, Jeffrey S. Kauanaugh, Paul H. Rogers, Christine Guillemin, Arthur Arnone, Frederic Galucteros, Claude Poyart, Jean Rosa, and Henri Wajcman

12820 Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. William Kastern, UlfSjabring, and Lars Bjorck 12851 Efficient plasma membrane expression of a functional platelet glycoprotein Ib-IX complex requires the presence of its three subunits. Jose A. Ldpez, Betty Leung, Clifford C. Reynolds, Chester Q. Li, and Joan E. E. Fox 12942 Sequence and specificity of a solublelactose-binding lectin from Xenopus laevis skin. Philippe Marschal, Jorg Herrmann, Hakon Leffkr, Samuel H. Barondes, and Douglas N. W. Cooper

12722 Characterization of /3-"'I-endorphin cross-linked proteins in NG108-15 cell membranes. A 26-kilodalton protein with propertiesof 8-opioid-binding site.Jane L. KO, Nancy M. Lee, and Horace H. Loh

13073 The epithelin precursor encodes two proteins with opposing activities on epithelial cell growth. GregoryD. Plowman, Jane11 M. Green, Michael G. Neubauer, Sharon D. Buckle Vicki L.McDonald, George J . Todaro, and Mohammed Shoyag

12742 Phosphorylation of the cystic fibrosis transmembrane conductance regulator. Marina R. Picciotto, Jonathan A.

13086 Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli. Eric D.Brown and Janet M. Wood

Cohn, Gloria Eertuzzi, Paul Greengard, and Angus C. Nairn 12813 Dissection of the /3 subunit in theEscherichia coli RNA polymerase into domainsby proteolytic cleavage. Konstantin Seuerinou, Arkady Mustaeu, Mikhail Kashleu, Sergei Borukhou, Vadim Nikiforou, and Alex Goldfarb

13114 Site-directed mutagenesis in rat liver 6-phosphofructo2-kinase mutation at the fructose 6-phosphate binding siteaffects phosphate activation. Vol. 267(1992) 4386-4393. Lin Li, Kai Lin,Irwin J . Kurlund,John J . Correia, and Simon J. Pilkis