BIOTECHNOLOGY MULTIPLE CHOICE QUESTIONS

APPLICATIONS OF BIOTECHNOLOGY

IN AGRICULTURE & IN MEDICINE

In this section we have studied the following:  Genetically modified crop plants  Genetically modified animals  Biofortification  Molecular diagnosis  Gene therapy  Gene manipulation through interference RNA

What is not true about the genetically modified ‘Bt crops’?

1) The technique has been applied to several species of crops 2) The source organism for the gene is a soil bacterium 3) The transferred gene exists in several allelic forms 4) Bt brinjal has been commercially exploited

What is not true about the genetically modified ‘Bt crops’?

1) The technique has been applied to several species of crops 2) The source organism for the gene is a soil bacterium 3) The transferred gene exists in several allelic forms 4) Bt brinjal has been commercially exploited

Golden rice is a genetically modified crop plant where the incorporated genes are meant for biosynthesis of -------. 1) vitamin A 2) vitamin C 3) vitamin B 4) Beta-carotene

Golden rice is a genetically modified crop plant where the incorporated genes are meant for biosynthesis of -------. 1) vitamin A 2) vitamin C 3) vitamin B 4) Beta-carotene

Which organism among the following was not used in the genetic engineering programme leading to the development of Golden Rice?

1) Escherichia coli

2) Erwinia uredovora 3) Agrobacterium tumefaciens

4) Narcissus pseudonarcissus

Which organism among the following was not used in the genetic engineering programme leading to the development of Golden Rice?

1) Escherichia coli

2) Erwinia uredovora 3) Agrobacterium tumefaciens

4) Narcissus pseudonarcissus

------- is the product of RNAi technique in genetic engineering. 1) Cry proteins of Bt cotton 2) Cry proteins of Bt brinjal 3) Beta carotene enriched Golden rice 4) Nematode resistance in tobacco

------- is the product of RNAi technique in genetic engineering. 1) Cry proteins of Bt cotton 2) Cry proteins of Bt brinjal 3) Beta carotene enriched Golden rice 4) Nematode resistance in tobacco

Interference RNA was discovered by 1) Cohen & Boyer 2) Fire & Mello 3) Yonath & Ramakrishnan 4) Holley & Khorana

Interference RNA was discovered by 1) Cohen & Boyer 2) Fire & Mello 3) Yonath & Ramakrishnan 4) Holley & Khorana

Identify the organism in which RNAi was first discovered.

1) Meloidegyne incognitia 2) Escherichia coli 3) Bacillus thuringiensis 4) Coenorhabditis elegans

Identify the organism in which RNAi was first discovered.

1) Meloidegyne incognitia 2) Escherichia coli 3) Bacillus thuringiensis 4) Coenorhabditis elegans

The function of Interference RNA to prevent a gene from functioning is also known as 1) Reverse transcription 2) Epigenetics 3) PTGS (Post Transcriptional Gene Silencing) 4) RNA splicing

The function of Interference RNA to prevent a gene from functioning is also known as 1) Reverse transcription 2) Epigenetics 3) PTGS (Post Transcriptional Gene Silencing) 4) RNA splicing

This is not a product of recombinant DNA technology: 1) Golden rice 2) Tracy 3) Bt cotton 4) Dolly

This is not a product of recombinant DNA technology: 1) Golden rice 2) Tracy 3) Bt cotton 4) Dolly

This is not a GMO. 1) Bt cotton 2) Golden rice 3) Tracy 4) Pomato

This is not a GMO. 1) Bt cotton 2) Golden rice 3) Tracy 4) Pomato

In the famous rDNA experiment to produce humulin, the substance was produced attached with another protein called -------. 1) beta-galactosidase 2) beta-galactoside 3) ampicillin 4) tetracycline

In the famous rDNA experiment to produce humulin, the substance was produced attached with another protein called -------. 1) beta-galactosidase 2) beta-galactoside 3) ampicillin 4) tetracycline

In the famous rDNA experiment to produce humulin, the substance was produced attached with another protein called -------. 1) beta-galactosidase 2) beta-galactoside 3) ampicillin 4) tetracycline

The active form of human insulin consists of these segments of polypeptide chains.

1) A & B Chains 2) A & C Chains

3) B & C Chains 4) A, B & C Chains

The active form of human insulin consists of these segments of polypeptide chains.

1) A & B Chains 2) A & C Chains

3) B & C Chains 4) A, B & C Chains

In the famous example of human gene therapy for SCID disease, the procedure adopted was: 1) ex vivo germ cell therapy 2) ex vivo somatic cell therapy 3) in vivo germ cell therapy 4) in vivo somatic cell therapy

In the famous example of human gene therapy for SCID disease, the procedure adopted was: 1) ex vivo germ cell therapy 2) ex vivo somatic cell therapy 3) in vivo germ cell therapy 4) in vivo somatic cell therapy

What is the advantage in clinical use of humulin (human insulin produced through rDNA technique) over use of conventional ox or pig insulin? 1) It does not cause immunological problems 2) It is cheaper for the patient 3) It is produced by E.coli in our own intestine 4) There is no advantage

What is the advantage in clinical use of humulin (human insulin produced through rDNA technique) over use of conventional ox or pig insulin? 1) It does not cause immunological problems 2) It is cheaper for the patient 3) It is produced by E.coli in our own intestine 4) There is no advantage

Gene therapy for SCID disease in man involves the transfer of the gene for this enzyme 1) Beta-galactosidase 2) Thrombokinase 3) Adenosine deaminase 4) Phenylalanine hydroxylase

Gene therapy for SCID disease in man involves the transfer of the gene for this enzyme 1) Beta-galactosidase 2) Thrombokinase 3) Adenosine deaminase 4) Phenylalanine hydroxylase

Spot the mismatch between the organisms with the actual modifications done: 1) Rosie – Lactalbumin enriched milk 2) Tracy – Human AAT protein enriched milk 3) GM Mice - Cry protein enrichment 4) Golden rice – beta-carotene enrichment

Spot the mismatch between the organisms with the actual modifications done: 1) Rosie – Lactalbumin enriched milk 2) Tracy – Human AAT protein enriched milk 3) GM Mice - Cry protein enrichment 4) Golden rice – beta-carotene enrichment

One of the advantages of developing transgenic mice is it is very useful in: 1) Keeping mice population under control 2) Producing new varieties of mice 3) Developing a show piece example 4) Gene targeting

One of the advantages of developing transgenic mice is it is very useful in: 1) Keeping mice population under control 2) Producing new varieties of mice 3) Developing a show piece example 4) Gene targeting

When these parts of a plant are used as explants in a tissue culture experiments the newly generated plants could be virus-free. 1) Pollen grains

2) Meristems 3) Parts of the embryo

4) Flower buds

When these parts of a plant are used as explants in a tissue culture experiments the newly generated plants could be virus-free. 1) Pollen grains

2) Meristems 3) Parts of the embryo

4) Flower buds

Haploid plants could be produced from tissue culture of the following parts of a healthy plant: 1) Pollen grains

2) Meristems 3) Parts of the embryo

4) Flower buds

Haploid plants could be produced from tissue culture of the following parts of a healthy plant: 1) Pollen grains

2) Meristems 3) Parts of the embryo

4) Flower buds

Normally this is not expected in the new plants produced through tissue culture programmes: 1) Vigorous growth 2) Quicker maturity 3) Formation of embryoids 4) Variations in genotype

Normally this is not expected in the new plants produced through tissue culture programmes: 1) Vigorous growth 2) Quicker maturity 3) Formation of embryoids 4) Variations in genotype

Tissue culture experiments have to be conducted in extremely aseptic conditions. Therefore all things used in the tissue culture programme must be properly sterilized before use with the exception of --------------.

1) 2) 3) 4)

The explant The culture vessel The various instruments used The nutrient medium

Tissue culture experiments have to be conducted in extremely aseptic conditions. Therefore all things used in the tissue culture programme must be properly sterilized before use with the exception of --------------.

1) 2) 3) 4)

The explant The culture vessel The various instruments used The nutrient medium

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