8TH INTERNATIONAL SCIENTIFIC CONFERENCE THE VITAL NATURE SIGN Abstract book 2014 Kaunas

ISSN 2335-8653

Scientific Committee 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

Prof. Habil. Dr. Audrius Maruška, Vytautas Magnus University, Lithuania Prof. Habil. Dr. Algimantas Paulauskas, Vytautas Magnus University, Lithuania Prof. Stellan Hjerten, Uppsala University, Sweden Prof. Hartmut Frank, University of Bayreuth, Germany Prof. Dr. Salvatore Fanali. Chromatography Institute of the CNR, Italy Prof. Dr. Jolanta Liesienė. Kaunas University of Technology, Lithuania Prof. Dr. Rimantas Venskutonis. Kaunas University of Technology, Lithuania Prof. Dr. Liudvikas Pranevičius, Vytautas Magnus University, Lithuania Prof. Dr. Vitalis Briedis, Lithuanian University of Health Sciences, Lithuania Prof. Dr. Douglas Westerlund, Uppsala University, Sweden Prof. Dr. Staffan Nilsson, Lund University, Sweden Prof. Dr. Isaak Rashal, University of Latvia, Latvia Prof. Olav Rosef, Telemark University College, Norway Prof. Dr. Ute Pyell, University of Marburg, Germany Prof. Dr. Pavel Jandera, University of Pardubice, Czech Republic Dr. Akos Vegvari. Lund University, Biochemical center, Sweden Dr. Susanne Wiedmer, University of Helsinki, Finland Dr. Anna Rocco, Chromatography Institute of the CNR, Italy

Organizing Committee Chairman/Editor: Prof. Habil. Dr. Audrius Maruška, Vytautas Magnus University, Lithuania 1. Dr. Ona Ragažinskienė. Vytautas Magnus university, Kaunas, Lithuania 2. Prof. Dr. Algirdas Raila. Aleksandras Stulginskis University, Kaunas Lithuania 3. Asoc. Prof. Dr. Saulius Šatkauskas, Vytautas Magnus University, Lithuania 4. Prof. Dr. Gintas Saulis, Vytautas Magnus University, Lithuania 5. Dr. Reda Kubiliūtė. Vilnius University, Lithuania 6. Dr. Vilma Ratautaitė, Vilnius University, Lithuania 7. Dr. Vykintas Baublys, Vytautas Magnus University, Lithuania 8. Dr. Saulius Mickevičius, Vytautas Magnus University, Lithuania 9. Tomas Drevinskas, Vytautas Magnus University, Lithuania 10. Kristina Bimbiraitė-Survilienė, Vytautas Magnus University, Lithuania 11. Mantas Stankevičius, Vytautas Magnus University, Lithuania 12. Ieva Akuneca, Vytautas Magnus University, Lithuania 13. Jurgita Mikašauskaitė, Vytautas Magnus University, Lithuania 14. Kęstutis Buivydas, Vytautas Magnus University, Lithuania 15. Dr. Violeta Bartkuvienė, Vytautas Magnus University, Lithuania 16. Academic youth society Modusas, Vytautas Magnus University, Lithuania

Abstract speech of authors were not edited © Vytautas Magnus University, 2014 BAR CODE 9772335865005

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L-1 THE USE OF OPTICAL STORAGE MEDIA FOR SURFACE PLASMON RESONANCE OBSERVATION Benas Gabrielis Urbonavičius, Diana Adlienė, Neringa Vaičiūnaitė L-2 ALTERNATIVE REFERENCE FRAMES IN WATER QUALITY ASSESSMENT OF THE MIDDLE DAUGAVA RIVER Dāvis Gruberts L-3 LACTIC ACID BACTERIA INDUCED GEOMETRICAL TRANS–CIS ISOMERISATION OF LYCOPENE FROM TOMATO Dalia Urbonaviciene, Elena Bartkiene, Daiva Vidmantiene, Grazina Juodeikiene, Pranas Viskelis L-4 ENANTIOMERIC SEPERATION OF 1-PHENYL-1,2,3,4-TETRAHYDROISOQUINOLINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Diāna Džabijeva, Igors Kļimenkovs, Jānis Ruško, Ilva Nakurte L-5 THE MAJOR PHYTOESTROGEN GROUPS – ISOFLAVONES, FLAVONES, COUMESTANS AND LIGNANS AND ITS FOOD SOURCES. DEFINITION AND HEALTH BENEFITS OF PHYTOESTROGENS. Elena Bartkiene L-6 USE OF FUNGAL LACCASE IN BIOREMEDIATION Enrica Donati, Chiara M. Polcaro, Emanuela Galli L-7 MYCOREMEDIATION OF POLLUTING SUBSTANCES BY BASIDIOMYCETES Emanuela Galli, Chiara M. Polcaro, Enrica Donati L-8 CAPILLARY ELECTROPHORESIS IN THE DIAGNOSIS OF SURGICAL SITE INFECTIONS E. Kłodzińska, R. Kubiliūtė, J. Leszczyński L-9 PLANT PHYSIOLOGY AND AGRONOMIC CHALLENGES: A REVIEW Giedrė Samuolienė, Pavelas Duchovskis, Aušra Brazaitytė, Akvilė Viršilė, Sandra Sakalauskienė, Jurga Miliauskienė, Ramūnas Sirtautas L-10 DETECTION OF TICK-BORNE PATHOGENS BY MOLECULAR METHODS Algimantas Paulauskas L-11 SCREENING,SELECTION AND TAXONOMIC IDENTIFICATION OF PAH RESISTANT FUNGI J. Mikašauskaitė, N. Tiso, V. Snieškienė, A. Stankevičienė, M.Stankevičius, T. Drevinskas, E. Galli, C.Polcaro, A. Maruška

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L-12 APPLICATION OF GIS MODELLING FOR THE ASSESSMENT OF ENVIRONMENTAL OFF-SITE IMPACTS OF SOIL EROSION AND SEDIMENT TRANSPORT FROM HEADWATER CATCHMENTS TO THE RIVER DAUGAVA Juris Soms L-13 ENANTIOSELECTIVE DETERMINATION OF ESSENTIAL α-AMINO ACIDS IN FERTILIZERS BY HPLC-MS/MS L. Taujenis, V. Olšauskaitė, A. Padarauskas L-14 SIMPLE UPLC-MS-TOF METHOD FOR NUCLEOTIDE POOL ANALYSIS IN BAKER’S YEAST CELLS (Saccharomyces cerevisiae) Marta Baumane, Janis Liepins, Juris Ķibilds, Agnese Kokina, Ilva Nakurte L-15 PHYSIOLOGICAL AND BIOCHEMICAL MECHANISMS OF PLANT ADAPTATION TO THE STRESS ACTION OF HEAVY METALS: IMPLICATIONS FOR THE PHYTOREMEDIATION TECHNOLOGY Massimo Zacchini, Fabrizio Pietrini, Valentina Iori, Daniela Di Baccio, Angelo Massacci L-16 THE EFFECTS OF NATURAL ANTIOXIDANTS ON PROCESS OF COMPLICATIONS IN PATIENTS WITH TYPE 2 DIABETES Nijole Savickiene, Asta Spadiene, Andrejs Skesters, Alise Silova, Antanas Norkus, Rytas Ostrauskas, Migle Francaite-Daugeliene L-17 TECHNIQUES OF SURFACE ANALYSIS: PRINCIPLES, TRENDS AND APPLICATIONS Saulius Kaciulis L-18 ANALYSIS OF BEE PRODUCTS USING CHROMATOGRAPHIC TECHNIQUES: A REVIEW Vilma Kaškonienė L-19 CONTACTLESS CAPACITIVE DETECTOR FOR CAPILLARY ELECTROPHORESIS Tomas Drevinskas, Audrius Maruška L-20 SUSTAINABILITY: BALANCING SCIENTIFIC (CHEMICAL) PROGRESS AND RISK Hartmut Frank L-21 CELLULOSE DERIVATIVES: SYNTHESIS AND APPLICATION Jolanta Liesiene

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P-1 GENETIC POLYMORPHISMS OF MITOCHONDRIAL GENOME D-LOOP REGION IN COMMON MERGANSER (MERGUS MERGANSER) OF LITHUANIA Auksė Abromaitytė, Aniolas Sruoga, Algimantas Paulauskas, Vaida Tubelytė, Vykintas Baublys P-2 COMPARISON OF LIGHT-EMITTING DIODES AND HIGH-PRESSURE SODIUM LAMPS LIGHTING EFFECTS ON TOMATO TRANSPLANTS Aistė Bagdonavičienė, Aušra Brazaitytė, Julė Jankauskienė, Sandra Sakalauskienė, Giedrė Samuolienė, Akvilė Viršilė, Jurga Miliauskienė, Ramūnas Sirtautas, Pavelas Duchovskis P-3 DETECTION OF CANINE BABESIOSIS CAUSATIVE AGENT BABESIA CANIS INDETRMACENTOR RETICULATUS TICKS IN LITHUANIA AND CENTRAL EUROPE Banaitytė Asta, Radzijevskaja Jana, Paulauskas Algimantas P-4 THE EFFECT OF LIGHT SPECTRUM COMPOSITION ON THE METABOLITE CONTENT IN LETTUCE AND BEET Alina Čeidaitė, Giedrė Samuolienė, Pavelas Duchovskis, Aušra Brazaitytė, Akvilė Viršilė P-5 THE RESPONSES OF GAS EXCHANGE PARAMETERS AND LEAF CHLOROPHYLL CONTENT TO ELEVATED CO2 AND DIFERENT SOIL N AVAILABILITY IN PISUM SATIVUM LEAVES Austra Dikšaitytė, Irena Januškaitienė P-6 THE EFFECTS OF GINKGO BILOBA L. AND CAMELLIA SINENSIS L. EXTRACTS ON DIABETES-RELATED DISTRESS AND QUALITY OF LIFE IN PATIENTS WITH TYPE 2 DIABETES A. Špadienė, L. Lašaitė, N. Savickienė, H. Rodovičius, G. Kazanavičius P-7 SMALL MAMMALIAN SUCCESSION AFTER FIRE IN PINUS BRUTIA (TEN.) FORESTS Kaynas, Burcin Y., and Gurkan, Behzat P-8 DISTRIBUTION OF DERMACENTOR RETICULATUS TICKS IN LITHUANIA Mardosaitė Dalytė, Radzijevskaja Jana, Paulauskas Algimantas P-9 HELMINTHS OF MUSTELIDS (MUSTELIDAE) IN LITHUANIA Dovilė Nugaraitė*, Vytautas Mažeika, Algimantas Paulauskas P-10 ANALYSIS OF ONCOGENE GLI1 PROTEIN EXPRESSION LEVELS BETWEEN DIFFERING GRADES OF ASTROCYTOMA Dovydas Gricius1,2, Arūnas Kazlauskas1 P-11 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY SEPERATION OF PSICHOACTIVES MEDICINS USING SOLID PHASE EXTRACTION FROM HUMAN URINE Daiva Kazlauskienė1 , Irina Halkevych2 P-12 CONCENTRATION OF NITRATE IN Spinacia oleracea, Eruca sativa, Dipotaxsis sp., Lactuca sativa L., Lactuca sativa var. capitata, Brasicca rapa ssp. pekinensis 5

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Dovilė Laurinavičienė, Lina Trečiokaitė, Karolina Žalynaitė P-13 PRESENCE OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING E. COLI ISOLATES IN HEALTHY SHEEP* F. Pehlivanoglu, H. Turutoglu, D. Ozturk P-14 THE USE OF ACINETOBACTER SP. FOR OIL HYDROCARBONS DEGRADATION IN SALINE WATERS Elnara Fatajeva, Artūras Juciūnas, Dainius Paliulis, Saulius Grigiškis P-15 STABLE CARBON ISOTOPE RATIO (δ13C) MEASUREMENT OF GRAPHITE USING EA-IRMS SYSTEM Edmundas Bružas , Andrius Garbaras, Vidmantas Remeikis P-16 NEEDLE NITROGEN CONCENTRATION OF LITHUANIA POPULATIONS OF JUNIPERUS COMMUNIS L. Edvina Krokaitė, Ramūnas Vilčinskas, Algimantas Paulauskas, Eugenija Kupčinskienė P-17 SYNTHESIS OF NEW 4-IMIDAZOLINONE COMPOUNDS Eligijus Kupriūnas, Jonas Salys, Liudas Šlepikas, Eduardas Tarasevičiu, Sigitas Tumkevičiu, Hiliaras Rodovičius P-18 THE INFLUENCE OF THE FERMENTED WITH CERTAIN LACTOBACILLI RHAPONTICUM CARTHAMOIDES ON THE QUALITY AND SAFETY OF CURD CHEESE Erika Skabeikyt, Giedre Jociene, Elena Bartkiene, Grazina Juodeikiene, Daiva Vidmantiene, Audrius Maruska, Ona Ragazinskiene P-19 EFFECT OF GIBBERELLIC ACID ON LIPID PEROXIDATION IN CHICKPEA (Cicer arietinum L.) SUBJECTED TO SALT AND BORON STRESSES Cekic Fazilet Ozlem P-20 SOME MULTILOCUS DNA MARKERS APPLICATION FOR ANALYSES OF NATIVE IN LITHUANIA POPULATIONS OF IMPATIENS NOLI-TANGERE L. Goda Sirutaviciute*, Lina Zybartaite, Ramunas Vilcinskas, Algimantas Paulauskas, Eugenija Kupcinskiene P-21 THE IMPACT OF AUXIN AND AUXIN INHIBITORS ON BARLEY MOMEOTIC MUTANTS AND THEIR HYBRIDS G. Kaselytė, V. Okockytė, R.Šiukšta, V. Vaitkūnienė P-22 OBSERVATION ON BIOLOGICAL PROPERTIES OF THE CHUB, Lueciscus cephalus AND ENVIRONMENT CONDITIONS IN THE ÇAPARLIPATLAK POND (BALIKESIR), SOUTHERN MARMARA REGION, TURKEY Hatice Torcu Koç , Zeliha Erdoğan , and Kazım Bulgen P-23 COMPOSTS WITH HAIR ADDITIVES QUALITY INFLUENCE ON RAPESEED GERMINATION 6

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Ieva Augunaite, Justa Sirvaityte, Virgilijus Valeika and Ilona Jonuskiene P-24 CHANGES IN CRUDE OIL HYDROCARBONS COMPOSITION, DURING DEGRADATION BY ARTHROBACTER SP. M1 AND ACINETOBACTER SP. PR82 in

optimal conditions Indrė Gailiūtė, Gražina Račkauskienė, Saulius Grigiškis P-25 THE DYNAMICS OF PHOTOSYNTHETIC PARAMETERS OF PHASEOLUS VULGARIS AND VICIA FABO UNDER STRONG CADMIUM STRESS Irena Januškaitienė P-26 PREVALENCE OF RICKETTSIA SPP. IN DERMACENTOR RETICULATUS TICKS IN LITHUANIA AND IN CENTRAL EUROPE Jonauskaitė Indrė, Banaitytė Asta, Radzijevskaja Jana, Paulauskas Algimantas P-27 ECTOPARASITES OF THE GLIS GLIS, MUSCARDINUS AVELLANARIUS AND DRYOMYS NITEDULA FROM LITHUANIA Indrė Lipatova, Simona Špakovaitė, Algimantas Paulauskas, Vaclovas Gedminas P-28 DETERMINATION OF CISPLATIN IN HUMAN URINE AND BLOOD PLASMA USING LIQUID CHROMATOGRAPHY-TIME OF FLIGHT MASS SPECTROMETRY Aija-Gerina-Berzina, Liene Jomerte, Uldis Vikmanis, Gunta Purkalne, Silvija Umbrashko,Kristina Bite, Kristine Trupa, Ruta Muceniece, Ilva Nakurte P-29 DETECTION OF BARTONELLA DNA IN WILD CERVIDS I.Pūraitė, A.Paulauskas, J.Radzijevskaja, O.Rosef, A.Sruoga P-30 THE PROTECTIVE EFFECTS OF ZINC IONS ON TOTAL PROTEINS AND METALLOTHIONEINS SYNTHESIS IN MICE LIVER Indrė Šveikauskaitė, Jurgita Šulinskienė, Ilona Sadauskienė, Leonid Ivanov P-31 RELATIONS BETWEEN NUTRITIONAL ELEMENTS IN THE VEGETATIVE ORGANS OF HYPERICUM MACULATUM GROWING WILD IN LITHUANIA Indrė Užubalytė, Irma Šliumpaitė, Lina Zybartaitė, Algimantas Paulauskas, Eugenija Kupčinskienė P-32 COMPARISON OF HYPERICUM MACULATUM CRANTZ POPULATIONS FROM LITHUANIA ACCORDING TO NON-ESSENTIAL ELEMENTS PROFILES IN THE ABOVEGROUND PARTS Indrė Užubalytė, Irma Šliumpaitė, Lina Zybartaitė, Algimantas Paulauskas, Eugenija Kupčinskienė P-33 GENETIC VARIABILITY OF AMERICAN MINK NEOVISON VISON IN LITHUANIA Inga Valanciute, Algimantas Paulauskas, Irma Puraite P-34 COMPOSITION OF BIOLOGICALLY ACTIVE COMPOUNDS OF BIOTECHNOLOGICALLY MODIFIED AND UNMODIFIED ANISE HYSSOP Jovita Bagočiūtė, Audrius Maruška, Mantas Stankevičius, Ona Ragažinskienė, Juozas Proscevičius, Kristina Bimbiraitė-Survilienė, Olga Kornyšova, Vilma Kaškonienė 7

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P-35 VARIATION OF PHYTOCHEMICAL MARKERS OF SOME LITHUANIAN ROSACEAE JUSS. SPECIES Julija Bajer, Simona Brazinskaitė P-36 THE SEVERITY OF ROOT ROT IN RED CLOVER GENETIC COLLECTION Jovita Bukauskaitė, Skaidrė Supronienė, Nijolė Lemežienė, Vida Danytė P-37 THE EFFECTS OF PHENOXY HERBICIDE MCPA ON NONTARGET VEGETATION IN SPRING WHEAT (TRITICUM AESTIVUM L.) CULTURE Jūratė Žaltauskaitė, Giedrė Kišonaitė P-38 COMPARISON OF DIFFERENT EXTRACTION METHODS OF RUTIN FROM LEAVES OF FAGOPYRUM ESCULENTUM MOENCH Justė Baranauskaitė, Vaidotas Žvikas, Valdas Jakštas P-39 DETERMINATION OF PROPOLIS VOLATILE COMPOUNDS BY GC-MS METHOD Jolanta Jasaitytė, Loreta Kubilienė, Mantas Stankevičius, Audrius Maruška P-40 PARASITES OF SIKA DEER (CERVUS NIPPON) Justina Monika Gibiežaitė, Irma Pūraitė, Vytautas Mažeika, Algimantas Paulauskas P- 41 GENETIC DIVERSITY OF WATER CROWFOOT SPECIES IN SOME LITHUANIAN RIVERS Jurgita Butkuvienė, Donatas Žvingila, Zofija Sinkevičienė P-42 BIOCHEMICAL, GENOTOXIC AND MIKROBIOLOGICAL PROPERTIES IN DIFFERENT VIBURNUM GENOTYPES A. Paulauskas, E. Kupčinksienė, J. Žukauskienė, R. Lazutka, G. Slapšytė, A. Stapulionytė , A. Paškevičius P-43 APPLICATION OF DOMINANT AND CODOMINANT DNA MARKERS FOR IMPATIENS GLANDULIFERA POPULATIONS FROM EASTERN BALTIC SITES Kriste Stravinskaite, Lina Zybartaite, Algimantas Paulauskas, Eugenija Kupcinskiene, Walter Durka P-44 DIVERSITY AND COMPOSITION OF TERESTRIAL ISOPODS (Crustacea: Isopoda) IN KAUNAS CITY E. Lelešius, I.Šatkauskienė, K.Kvašnauskaitė P-45 TICK BORN PATHOGENS IN RODENTS IN LITHUANIA Karolis Sivickis, Jana Radzijevskaja, Vaclovas Gedminas, Algimantas Paulauskas P-46 PHYTOTOXICITY COMPARISON OF TWO INVASIVE HERACLEUM SPP. L. Baležentienė

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P-47 EVALUATION OF FRUITING POTENTIAL AND BERRY QUALITY OF KOLOMIKTA KIWI ACTINIDIA KOLOMIKTA Laima Česonienė, Remigijus Daubaras P-48 DETERMINTION OF PHENOLIC COMPOUNDS IN GINKGO BILOBA L. METANOLIC EXTRACTS BY TLC SCANNING Laura Rimkienė, Liudas Ivanauskas, Valdas Jakštas, Rūta Marksienė, Guoda Kiliuvienė, Agnė Pociūtė P-49 THE SORPTION OF ANTHRAQUINONE DYE INTO E. COLI BACTERIA AND THE DYE IMPACT ON THE BACTERIA VIABILITY Monika Gavrilenkaite, Lina Rageliene

P-50 THE EFFECTS OF LIPOPHILIC COMPOUNDS ON MULTIDRUG RESISTANCE PUMPS IN JURKAT AND MX1 CELLS Mantas Riauka, Rimantas Daugelavičius P-51 DEVELOPMENT OF HPLC METHOD FOR EVALUATION OF BIOLOGICALLY ACTVE COMPOUNDS IN SOLIDAGO L. HERBAL EXTRACTS Mindaugas Marksa, Liudas Ivanauskas, Valdas Jakštas, Jolita Radušienė, Rūta Marksienė, Simona Kamandulytė, Kristina Sendrauskaitė P-52 COMPARISON OF TWO SPE SORBENTS FOR A RESIDUE ANALYSIS OF ZALEPLON AND ZOPICLONE BY FAST GAS CHROMATOGRAPHY TECHNIQUE Nerijus Karlonas, Arunas Ramanavicius, Almira Ramanaviciene P-53 ANTISYMMETRIZATION IN IDENTICAL MANY FERMION SYSTEMS Osvaldas Jankauskas P-54 PULSED ELECTRIC FIELD ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM BLUEBERRIES Ramunė Bobinaitė, Saulius Šatkauskas, Nerijus Lamanauskas, Pranas Viškelis, Gianpiero Pataro, Giovanna Ferrari P-55 EVALUATION OF POPULATIONS OF INVASIVE IN LITHUANIA IMPATIENS PARVIFLORA ACCORDING TO SET OF MULTILOCUS MARKERS Rasa Janulionienė, Algimantas Paulauskas, Eugenija Kupčinskienė P-56 EVALUATION OF SYNERGY OF ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS AND PHENOLIC COMPOUNDS EXTRACTED FROM ANGELICA ARCHANGELICA L. SPECIES Rūta Mickienė, Anika Friese, Uwe Rosler, Audrius Maruška, Ona Ragažinskienė P-57 THE USE OF MEMBRANE FILTRATION PROCESS FOR PUMPKIN JUICE FRACTIONATION R. Staišiūnaitė, D. Tamulionienė, E. Damašienė, V. Kaškonienė, J. Damašius P-58 PAST, PRESENT AND FUTURE: THE CASE OF HIGHLY INVASIVE SNAIL POTAMOPYRGUS ANTIPODARUM (J. E. GRAY, 1853) 9

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Rokas Butkus P-59 VIBURNUM OPULUS – A NEW PLANT FOR FOOD AND PHARMACEUTICAL INDUSTRY Remigijus Daubaras, Laima Česonienė, Vilma Kraujalytė, Petras Rimantas Venskutonis, Audrius Pukalskas, Antanas Šarkinas P-60 THE INFLUENCE OF 3-(1H-BENZIMIDAZOL-2-YL)-4-PHENYLAMINOBUTANOIC ACID SODIUM SALT ON SUMMER RAPESEED (BRASSICA NAPUS L.) GROWTH AND YIELD R. Kolosej, V. Mickevičius, I. Jonuškienė, Z. Brazienė P-61 RELATION BETWEEN EXPOSURE TIME IN POLLUTED SOIL EXTRACTS AND STAMEN HAIR MUTATION FREQUENCY OF Tradescantia CLONE 4430 S.Bondzinskaite P-62 DOMINANT DNA MARKERS BASED COMPARISON OF POPULATIONS OF IMPATIENS PARVIFLORA INVASIVE IN BALTIC DISTRICT Simona Grusaite, Ramunas Vilcinskas, Lina Zybartaite, Daiva Ambrasiene, Algimantas Paulauskas, Eugenija Kupcinskiene P-63 GENETIC DIVERSITY AND POPULATION STRUCTURE OF RACCOON DOG (NYCTEREUTES PROCYONOIDES) ANALYSIS IN LITHUANIA Simona Juknelytė, Loreta Griciuvienė, Algimantas Paulauskas, Jana Radzijevskaja, Vaclovas Gedminas P-64 EVALUATION OF BIOREMEDIATION OF USED WOODEN RAILWAY TIES S. Kraskauskaitė, A. Maruška, J. Mikašauskaitė, M. Stankevičius, T. Drevinskas, N. Tiso P-65 METHOD DEVELOPMENT FOR PIMOBENDAN ENANTIOMERIC SEPERATION USING CHIRALLIQUID CHROMATOGRAPHY Santa Niedra, Toms Rekis, Andris Actins, Ilva Nakurte P-66 CEMETERIES AS POTENTIAL DONOR TERRITORIES OF NEOPHYTE PLANT SPECIES: CASE STUDY IN DAUGAVPILS (LATVIA) Santa Rutkovska, Irēna Pučka P-67 SCREENING OF CHEMICAL COMPOSITION OF SUMMER SAVORY ESSENTIAL OILS USING SOLID-PHASE MICROEXTRACTION AND GC-MS Simona Šuštauskaitė, Zita Barsteigienė, Audrius Maruška, Ona Ragažinskienė, Mantas Stankevičius, Erika Šeiniauskienė P-68 SYNTHESIS OF NOVEL 2-AMINOTHIAZOLE DERIVATIVES Violeta Bartkuviené P-69 MIGRATING AND HATCHING IN NESTS BURROWS PASSERINE BIRDS INFESTATION WITH IXODES SPP. TICKS IN LITHUANIA 10

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Vesta Matulaityté, Eglé Galdikaité, Algimantas Paulauskas, Jana Radzijevskaja P-70 ASSESSMENT OF THE ALLELOPATHIC POTENTIAL OF INVASIVE SPECIES DAISY FLEABANE Virginija Tunaitienė, Karolina Krivičiūtė, Jolanta Patamsytė, Tatjana Čėsnienė, Violeta Kleizaitė, Donatas Žvingila P-71 ANTIOXIDANT PROPERTIES OF OCIMUM BASILICUM (L.) MICROGREENS GROWN UNDER SUPPLEMENTAL SHORT-TERM RED LED LIGHTING Viktorija Vaštakaitė, Aušra Brazaitytė, Akvilė Viršilė, Julė Jankauskienė, Giedrė Samuolienė, Sandra Sakalauskienė, Algirdas Novičkovas, Ramūnas Sirtautas, Jurga Miliauskienė, Pavelas Duchovskis P-72 COMPARISON OF EXTRACTION OF BEE POLLEN VOLATILES USING DIFFERENT SPME FIBERS AND GC-MS ANALYSIS Vilma Kaškonienė, Geralda Ruočkuvienė, Audrius Maruška P-73 MYCOTOXINS REDUCING IN CEREAL BY- PRODUCTS BY USING FERMENTATION Vita Krungleviciute, Elena Bartkiene, Jone Kantautaite, Grazina Juodeikiene, Violeta Baliukoniene, Bronius Bakutis P-74 Genetic diversity of Lithuania populations of Juniperus communis L. in relation to habitat differences Ramūnas Vilčinskas, Vitas Marozas, Lina Zybartaitė, Algimantas Paulauskas, Eugenija Kupcinskiene P-75 INTRODUCTION OF Scutellaria baicalensis GEORGI AND PHYTOCHEMICAL ANALYSIS OF NON-VOLATILE COMPOUNDS IN LITHUANIA Alice Corbett, Mantas Stankevičius, prof. habil. dr. Audrius Maruška, prof. dr. Ona Ragažinskienė

P-76 THE IMPACT OF INTRODUCTION ON THE PHYTOCHEMICAL COMPOSITION OF CHAMERION ANGUSTIFOLIUM (L.) HOLUB Vilma Kaškonienė, Mantas Stankevičius, Tomas Drevinskas, Audrius Maruška, Ona Ragažinskienė, Rasa Ugenskienė and Vitalis Briedis P-77 DETERMINATION OF LIPOPOLYSACCHARIDE BY SPECTROPHOTOMETRIC AND POTENTIOMETRIC METHODS SANDRA KMIELIAUSKAITĖ, LINA RAGELIENĖ Vytautas Magnus University, Department of Biochemistry and Biotechnologies, Vileikos 8, LT- 44404 Kaunas, Lithuania

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L-1 THE USE OF OPTICAL STORAGE MEDIA FOR SURFACE PLASMON RESONANCE OBSERVATION Benas Gabrielis Urbonavičius, Diana Adlienė, Neringa Vaičiūnaitė Kaunas University of Technology, Physics Department, Studentų str. 50, LT-51368 Kaunas The surface plasmon resonance (SPR) is one of the effects occurring at the metal interface when it interacts with incident light. Resonance can be observed at certain incident angle. This angle is highly dependent on the properties of the metal surface. This dependency can be used as a base for biological or chemical sensors. A few different methods are used to make use of this effect, including Kretschmann and Otto optical configurations, optical fiber coupling and diffraction grating-like metal surface. Metals like gold, silver, copper and aluminum are usually used in sensor devices, because of their sharp resonance peaks in near-infrared and visible parts of the electromagnetic spectrum. Gold is the most preferred one, because of its high chemical stability. Fabrication of thin-film uniform gold structures can be costly or even impossible. Some optical storage media manufacturers use gold as the reflective layer in their products. Since the structure of an empty optical storage media disk is similar to a diffraction grating these disks can be used as a medium for SPR sensors, which is low-cost and widely available. Possibilities to expose the gold coated diffraction grating in CD/DVD and some of their properties as SPR medium are discussed as well as the possible use of HD-DVD and BR disk structures.

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L-2 ALTERNATIVE REFERENCE FRAMES IN WATER QUALITY ASSESSMENT OF THE MIDDLE DAUGAVA RIVER Dāvis Gruberts Department of Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, Daugavpils, Latvia

Eulerian and Lagrangian sampling strategies are alternative methods for the in-situ investigations of water quality in large rivers. According to the Eulerian sampling strategy, water quality monitoring is traditionally performed at fixed sampling stations along the river. In this case, the water quality time series are obtained for the particular locations. Alternatively, when using the Lagrangian method, the selected water ‘parcel’ is followed down the river channel and repeated measurements of its physical and chemical properties are performed at different locations. Therefore, downstream transformation processes of the selected water quality parameters could be recorded and investigated in a real-time regime. Results of several real-time Lagrangian drift experiments conducted on the Middle Daugava River (South-East Latvia) during the spring floods in 2007-2013 are reviewed in a context of water quality assessment. The selected parameters include pH, DO, BOD5, NH4, total N and total P. Possible impact of municipal wastewaters from Daugavpils City, as well as the flow hydrodynamics and other environmental factors on these parameters are analysed and discussed from both, Eulerian and Lagrangian perspectives.

Keywords: Eulerian and Lagrangian reference frames, drift experiments, in-situ sampling, water quality assessment.

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L-3 LACTIC ACID BACTERIA INDUCED TRANS–CIS ISOMERISATION OF LYCOPENE FROM TOMATO

GEOMETRICAL

Dalia Urbonavicienea,b*, Elena Bartkienec, Daiva Vidmantienea, Grazina Juodeikienea, Pranas Viskelisb a

Kaunas University of Technology, Kaunas, LT-47181, Lithuania Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, LT-54333, Lithuania c Lithuanian University of Health Sciences Veterinary Academy, Kaunas, LT-47181, Lithuania Dalia Urbonavicienė*:+37068308157; [email protected] b

In our days the development of more attractive functional food are important for the consumers’ health [1]. Tomato is a important product of establishing functional food. Tomato and related tomato products are the major source of natural antioxidant lycopene [2]. Probiotication is one of the methods used to produce fermented functional foods [3]. The aim of this study was to evaluate the impact of fermentation with Pediococus pentosaceus MI807, Lactobaccilus sakei and spontaneous sourdough on the lycopene and -carotene content in tomato powder. The products were fermented with Pediococus pentosaceus (+35 °C; 4 days), Lactobaccilus sakei (+30 °C; 4 days) and spontaneous sourdough (+ 30°C; 4 days). Total lycopene and -carotene content also lycopene isomers were identified using high pressure liquid chromatography method with an UV detector. Results of the study show that the amount of -carotene and lycopene in fermented tomato powder with Pediococus pentosaceus MI807, Lactobaccilus sakei were not significantly diference (10,73 mg/100g and 86,54 mg/100g respectively) ((p>0.05). Amount of -carotene and lycopene in fermented tomato powder with spontaneous sourdough was the 44 % lower compered to Pediococus pentosaceus MI807, Lactobaccilus sakei sourdough. Both Pediococcus acidilactici MI807 and Lactobaccilus sakei may be used in the fermentation of the analysed products. Key words: tomato, fermentation, lycopene, -carotene, health References: 1. Kaur S, M Das M (2011).Food Sci Biotechnol 20 (4): 861-875. 2. Riccioni G, Mancini B, Di Ilio E, Bucciarelli T, D’Orazio N (2008). Eur Rev Med pharmacol Sci 12 (3): 8390. 3. Gardner NJ, Savard T, Obermeier P, Caldwell G, Champagne CP (2001). Int J Food Microbiol 64: 261-275.

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L-4 ENANTIOMERIC TETRAHYDROISOQUINOLINE CHROMATOGRAPHY

SEPERATION BY HIGH

OF 1-PHENYL-1,2,3,4PERFORMANCE LIQUID

Diāna Džabijeva1, Igors Kļimenkovs1, Jānis Ruško1, Ilva Nakurte1 1

University of Latvia, Faculty of Chemistry, Kr. Valdemara street 48, Riga LV 1013, Latvia

E-mail: [email protected]

Isoquinolines are the wide- spread chemical compounds used in medicine for treating different diseases. The derivatives of 1-phenyl-1,2,3,4- tetrahydroisoquinoline are used in the treatment of tuberculosis, arthritis, rheumatism, malaria and human immunodeficiency virus (HIV). Usually only one enantiomeric form is biologically active, that is why it is very important to separate the enantiomers of 1-phenyl-1,2,3,4- tetrahydroisoquinoline. The purpose of this research was to develop a Liquid Chromatography (LC) method for the separation of a biologically active S isomer from inactive R, using glycoprotein ovomucoid as a chiral selector. It’s worth saying, that using asymmetric stationary phase, there is no need to derivate the components of racemic mixture, as separation is obtained as a result of weak interactions between isomer and sorbent. These interactions mostly are steric factors, hydrophobic and electrostatic interactions. Chromatographic analyses were performed on a modular UPLC system, Agilent 1290 Infinity series (Agilent Technologies). The separation of enantiomers was achieved by using an ULTRON ESOVM column (4.6 150 mm, 5 µm) with a mobile phase composed of 96% 20 mM KH2PO4 pH 5.5 and 4% EtOH in isocratic mode at a flow rate of 0.9 mL/min. The detection was monitored at 205 nm. Successful application of the method for the separation of enantiomers of 1-phenyl-1,2,3,4tetrahydroisoquinoline illustrates the usefulness of the method and lets to conclude, that it is suitable for usage in industrial drug synthesis. The authors would like to thank Olainfarm scholarship for financial support and research fellowship (D. D.) to perform this research.

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L-5 THE MAJOR PHYTOESTROGEN GROUPS – ISOFLAVONES, FLAVONES, COUMESTANS AND LIGNANS AND ITS FOOD SOURCES. DEFINITION AND HEALTH BENEFITS OF PHYTOESTROGENS. Elena Bartkiene1 1

Lithuanian University of Health Sciences, Tilzes g. 18, 47181 Kaunas, Lithuania

Recently special attention has been paid to dietary fibre associated phytochemicals such as phytoestrogens. Epidemiological- and in vitro fermentation- studies and experiments with animals have suggested that the biologically activity of these compounds are associated with the prevention of different diseases. Till now phytoestrogens in cereals, legumes and oilseeds, especilally in rye, soy beans and flaxseed have been generally studied. According to researchers, a high daily intake of plant lignans is probably important for the intestinal conversion of plant lignans to the mammalian lignans enterodiol and enterolactone. Enterodiol and enterolactone decrease the risk of hormonedependent diseases, such as breast and prostate cancer, because of hormone level changes are also important for the prevention of coronary heart disease. There are different opinions about the influence of soy isoflavones and their metabolites on human health. One group of researchers explain that the influence of soy isoflavones on humans has an anti-cancer effect while the other group advocates an opposite view. In the human organism soy isoflavones seem to work like estrogens and seem to stimulate hormone-dependent diseases. Therefore, dietary fibre and with them the associated phytoestrogens and their metabolites are actual the object of investigation. Special attention should also be paid to less investigated plant products which could be rich sources of phytoestrogens. In this area a lot of research has to be carried out to understand the phytoestrogens role in the disease prevention mechanism and to determine the recomended daily intake. Further solutions of the problem are linked with the development of new methods of investigation. Keywords: phytoestrogens, phytoestrogens food sources, health benefits of phytoestrogens

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L-6 USE OF FUNGAL LACCASE IN BIOREMEDIATION Enrica Donati1, Chiara M. Polcaro1, Emanuela Galli2 1-Institute of Chemical Methodologies (IMC), 2- Institute of Agro-Environmental and Forest Biology (IBAF) CNR - Research Area of Rome1, via Salaria Km 29.300 – 00015 Monterotondo (Rome) Italy.

Laccases are a class of Cu-containing enzymes able to oxidize polyphenols and many other compounds by oxygen reduction. They are glycoproteins with a MW ranging from 60 to 70 KDa and a E0 between 450 and 800 mV. Ligninolytic white-rot fungi are the most important source of laccases, mainly produced as extracellular enzymes. Their most diffused industrial application is biopulping and biobleaching, in paper and cotton manufacture. Owing to their availability and wide versatility, which can be further extended by the use of “mediators”, these enzymes are also suitable for “green” bioremediation and have demonstrated to degrade efficiently various environmental pollutants. Laccases are able to degrade phenols, among which Personal Care Products as nonylphenol, chloro and nitrophenols. They are also frequently used in degradation of dyes, steroid hormones and antibiotics, aromatic amines and eterocyclic compounds. Polyciclic Aromatic Hydrocarbons degradation usually requires the presence of a mediator, but low molecular weight PAH, as Naphtalene, can be degraded directly. With a mediator addition, laccases are also able to decolorize fungal or bacterial pigments. In order to perform efficient systems for water depuration and improve enzyme stability, many studies about the supporting of laccase on solid materials have been carried out. The enzymes have been included in gels (Eupergit) or chemically linked to acrylonitrile resins or a number of silica based materials (microparticulate silica, magnetic nanomaterials). When a bioremediation system is performed, an equilibrium among efficiency, cost and bioreactor practical realization must be considered. So laccases have also been adsorbed or chemically linked to natural materials such as spent grain, chitosan, diatomaceous biosilica, celite and zeolite (clinoptilolite). Some applications of free and immobilized laccase in the bioremediation of different compounds will be reported.

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L-7 MYCOREMEDIATION OF POLLUTING SUBSTANCES BY BASIDIOMYCETES Emanuela Galli1, Chiara M. Polcaro2, Enrica Donati2 1- Institute of Agro-Environmental and Forest Biology (IBAF), 2- Institute of Chemical Methodologie (IMC) CNR - Research Area of Rome1, via Salaria Km 29.300 - 00015 Monterotondo (Rome) Italy.

Mycoremediation is a form of bioremediation that uses fungal mycelia to return an environment contaminated by pollutants to a less contaminated state. One of the primary role of fungi in the ecosystem is decomposition which is performed by the mycelium. White-rot fungi secrete extracellular enzymes and acids that breakdown lignin and cellulose, the two main building blocks of plant fiber. The ligninolytic enzymes have low substrate specificity and that permits them the degradation of organic xenobiotic compounds with different origin, scarcely susceptible to bacterial attack. The key to mycoremediation is determining the right fungal species to target a specific pollutant. In IBAF laboratories a fungal germplasm bank is present (International Bank of Edible Saprophytic Mushrooms), containing about 530 samples of wood-degrading fungi, mainly Pleurotus. Fungi belonging to Pleurotus genus have been used for the treatment of polluted waste for many years. In particular, olive mill waste waters were used as growth medium for P. ostreatus, with the result of an abundant mycelium growth and the decrease of COD and phenols in the waste waters. P. ostreatus was able to grow in a mixture containing wood treated with creosote (CTW) and wheat straw, totally degrading phenols and reducing the 65-70% of polycyclic aromatic hydrocarbons and heterocyclic compounds. To clarify the degradation of polyciclic aromatic hydrocarbons (PAH), a study was performed in batch on fluoranthene, one of the principal PAH contained in creosote. The results pointed out that this compound was totally absorbed by the mycelium (15 days) and then degraded by 50% in 40 days. Also the antibiotic Oxytetracycline, that enters the environmental compartments as a consequence of animal waste disposal, disappeared in P. ostreatus culture medium as early as the third day of exposure onwards, with an almost complete removal after 14 days.

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L-8 CAPILLARY ELECTROPHORESIS IN THE DIAGNOSIS OF SURGICAL SITE INFECTIONS E. Kłodzińska1, R. Kubiliūtė2, J. Leszczyński1 1

Institute for Engineering of Polymer Materials and Dyes, M. Sklodowskiej-Curie 55, 87-100 Torun, Poland, tel: 0048 (56) 650 00 44, fax: 0048 (56) 650 03 33 e-mail: [email protected] 2 State research institute Center for Physical Sciences and Savanorių ave. 231, LT-02300 Vilnius, Lithuania

Technology

A surgical site infection (SSI) is an infection that occurs after surgery in the part of the body where the surgery took place. An SSI may range from a spontaneously limited wound discharge within 7– 10 days of an operation to a life-threatening postoperative complication, such as a sternal infection after open heart surgery. Most SSIs are caused by contamination of an incision with microorganisms from the patient’s own body during surgery. From the analytical point of view, the complex nature of these samples as well as the low concentrations of analytes require a system with high sensitivity and efficiency. Such situation requires a technique such as CE, which is a powerful and versatile separation technique that promises to rival HPLC when applied to the separation of both charge and neutral species. During the study, it has been demonstrated that CZE identifies characteristics of such groups of pathogens such as bacteria Gram (+) and different species of bacteria Gram (−), and also develops weekly individual profiles for patients after application of antibiotics. This was done in order to show the impact of antibiotic therapy in change “numbers” of bacteria present in the wound after surgery. The metod proved to be the ideal straight specificity in the case of Escherichia coli (100%). Finally, analysis of the spectra and the second derivatives of the UV-Vis spectra confirmed the similarity in the profiles and showed that the CZE is a great method for fast screening test in bacterial infection. It can be concluded that compared to traditional phenotypic methods, the present process of identification has some undeniable advantages: 

 

 

Direct analysis of biological samples. In the case of application of CZE, contaminated material can be directly introduced into the system. It does not require any sample preparation. Short analysis time. The results are achieved after 15– 45 min. Interpretation of the result can bemade in various ways. Sensitivity and repeatability. Based on the analyzed patients with wound infection, we were able to obtain an overall sensitivity of the method in the order of 88.5% in the case of mixtures of different species of bacteria. By creating weekly profiles for each patient, we showed that electrophoresis is also a good method to evaluate the effectiveness of antibiotic therapy [6]. Analysis of the spectra and the second derivatives of the UV-Vis spectra confirmed the similarity in the profiles and showed that CZE is a great method for fast screening test in bacterial infection.

This work was financially supported by theMinistry of Science and Higher Education (grant no. 3694/B/H03/2011/40). 19

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L-9 PLANT PHYSIOLOGY AND AGRONOMIC CHALLENGES: A REVIEW Giedrė Samuolienė, Pavelas Duchovskis, Aušra Brazaitytė, Akvilė Viršilė, Sandra Sakalauskienė, Jurga Miliauskienė, Ramūnas Sirtautas Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas str. 30, LT-54333 Babtai, Kaunas dist., Lithuania

The development of agronomic research ensures the public demand for vegetable food, feed and industry raw materials. Plant physiology is considered to be theoretical background for agronomy (crop production and horticulture). Knowledge of plant physiology patterns enables to control formation of productivity elements during organogenesis and quality of production. Plant biological productivity is formed due to photosynthesis. Management of crop photosynthetic indices by technological measures is very important and allows enhance plant productivity and quality of production, decrease negative influence of changing climate and anthropogenic activity on crop production and horticulture. The knowledge of morphogenesis pattern enables to control the ratio between different plant organs, to influence biologic productivity, to divert assimilates to commercially useful organs. It is important to know peculiarities of plant flowering, as eatable organ of most plants is formed from flower or inflorescence. Plant lighting is very important for controlled environment agriculture. Lighting technology, such as light-emitting diodes (LEDs), which allows reducing costs and satisfies plant need is in great interest. Only during recent years appeared LEDs which spectral composition is suitable for plant photoacceptor system needs, it allows optimizing plants’ photosynthetic and photomorphogenetic processes, influence metabolic pathways and at the same time to influence quality of production. Thus such plant physiology researches allows know plant functioning mechanisms, control processes of growth and development, realize potential of biological productivity. Keywords: metabolism, flowering, vernalization, light-emitting diodes, productivity, stress

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L-10 DETECTION OF TICK-BORNE PATHOGENS BY MOLECULAR METHODS Algimantas Paulauskas Faculty of Natural Sciences, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas, Lithuania E-mail: [email protected]

Over the past two decades, tick-borne diseases have increased and now constitute a major health problem in many parts of Europe and North America, accounting for tens of thousands of new cases yearly in both regions. The most common of these diseases are tick-borne encephalitis, Lyme disease, anaplasmosis and babesiosis. According to the data of World Health Organization, in Lithuania the incidence of tick-borne encephalitis and Lyme boreliosis has markedly increased since 1990 and is the highest in Northern Europe. The study and identification of tick-transmitted pathogens is complicated as require investigation of all levels of the pathogen–host system – the pathogen, its competent vector and the reservoir host. Several methods have been developed for a direct detection of pathogens in infected vectors, host tissue, and clinical specimens from a patient. These include microscope-based assays, antigen detection assays, in vitro cultivation, and nucleic acid-based detection. However, detection methods such as culture isolation, dark-field microscopy and indirect antibody and antigen detection assays, are either time-consuming or prone to contamination, or have limited specificity and sensitivity. Using only phenotypic methods, it is impossible to resolve genetic differences among the different species of pathogens. In the recent years, molecular detection methods based on PCR amplification of the DNA of the pathogen have been shown to be effective for the diagnosis of tick-borne diseases and for elucidation of their epidemiology. Various polymerase chain reaction-based molecular methods, such as nested PCR, RFLP/PCR, Multiplex PCR, Real-time PCR, Real-time multiplex PCR, DNA–DNA hybridization analysis, nucleic acid sequence analysis have shown an increasing significance in the detection and typing of bacterial pathogens and parasitic protozoa. These above-mentioned detailed researches have been started recently and have successfully extended in different countries of the world. In Lithuania, scientific research studies dedicated to molecular detection of tick-borne pathogens in invertebrate vectors and vertebrate hosts start to develop. During the last decade, molecular detection and identification of causative agents of tick-borne encephalitis, Lyme boreliosis, anaplasmosis and babesiosis in ticks and their hosts was carried out. Acknowledgements: The study was partially supported by the Research Council of Lithuania (grant no. MIP-053/2013).

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L-11 SCREENING, SELECTION AND TAXONOMIC IDENTIFICATION OF PAH RESISTANT FUNGI J. Mikašauskaitė1, N. Tiso1, V. Snieškienė2, A. Stankevičienė2, M.Stankevičius1, T. Drevinskas1, E. Galli4, C.Polcaro4, A. Maruška1 Department of biochemistry and biotechnologies, Vytautas Magnus University, Vileikos 8, LT-44404, Kaunas, Lithuania 2 Kaunas Botanical Garden, Vytautas Magnus University, Z. E. Zilibero st. 6, Kaunas LT-46324 Kaunas, Lithuania 4 National Research Council (CNR), Istitute of Agroenvironmental and Forestal Biology (IBAF) & Institute of Chemical Methodologies (IMC), Area della Ricerca di Roma, Via Salaria Km 29,300, 00015, Monterotondo (Roma), Italy 1

Mycoremediation is a form of bioremediation, which exploits fungi in pollutant isolation and degradation. Application of the mycoremediation for decontamination is one of effective waste disposal options. Polycyclic aromatic hydrocarbons (PAH) are one of the main components of coal tar, which is used for impregnation of wooden railway sleepers as it is efficient wood preservative against fungi. Some species of fungi have ability to degrade or metabolize PAH. In general, fungal metabolites are less toxic than primary hydrocarbons. The discovery of PAH degrading fungi begins with determination of PAH resistant fungal species. The aim of this research comprises: 1. Screening of fungi found on the railway sleepers and surroundings in Lithuania; 2. Fungi identification; 3. Determination of efficiency of different fungal species for degradation of PAH. Five different species of fungi (Pleurotus ostreatus SMR 684, Pleurotus eryngii VMU001, Bjerkandera adusta VMU004, Irpex lacteus VMU003 and Schizophyllum commune VMU002) demonstrate high resistance to different coal tar concentrations. Composition of coal tar in railway wooden sleepers was determined using GC-MS, GC-FID and UPLC instrumental analysis methods. Acknowledgement: This project is financed by EUSFA, grant No VP1-3.1-ŠMM-10-V-02-010 (BIOREM). Keywords: Pleurotus ostreatus, Pleurotus eryngii, GC-MS, mycoremediation, PAH, coal tar.

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L-12 APPLICATION OF GIS MODELLING FOR THE ASSESSMENT OF ENVIRONMENTAL OFF-SITE IMPACTS OF SOIL EROSION AND SEDIMENT TRANSPORT FROM HEADWATER CATCHMENTS TO THE RIVER DAUGAVA Juris Soms Department of Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, Daugavpils, Latvia Soil erosion by water, beside its direct on-site negative effects like decreasing the productivity of soils and degradation of agricultural lands, in addition has various negative off-site environmental impacts due to sediment delivery from small headwater catchments into nearby rivers and lakes. Simultaneously, transferring of soil erosion products from uplands to permanent water bodies reduces water quality due to inflow of pollutants such as nutrients and agrochemicals. All these adverse impacts aggravate and accelerate processes of siltation, pollution and eutrophication. Hence soil erosion in terms of protection of water resources in many EU countries is ranked among other environmental problems. Moreover, these matters may become a more serious problem throughout the world, also in the countries of the Baltic Region, e.g. Latvia, considering the potential influence of global climate change on formation of surface runoff and soil erosion processes. Thereby it is essential to carry out studies focused on these issues in order to obtain reliable data in terms of both scientific and applied aims, e.g. environmental protection and sustainable management of water resources. One of the topics of such studies is the soil erosion modelling by GIS, and this approach currently is widely used all over the world as it is reflected by research results published in the scientific literature. This approach also inspired author to realize the assessment of potential soil erosion rates and sediment transport in south-eastern Latvia by application of GIS-based modelling, which is presented in this paper. For research purposes empirical USLE model and ArcGIS software were applied and headwater catchments located in the river Daugava valley were chosen as model territories. First of all, the USLE input data for modelling were generated as thematic ESRI grid format raster layers. After that ArcMap 9.3.1 software was used to assess the soil loss and its spatial distribution. Obtained results indicate that potential sediment transport from headwater catchments vary from 0 to 195 t ha-1 yr-1; in average for the whole territory under study this value is 0.571 t ha -1 yr-1. Despite the fact that these values are rather low in comparison to the suspended sediment annual yield of the river Daugava in this stretch, the high number of headwater catchments can supply significant amount of eroded material to the receiving stream, hence playing important role in the processes related to siltation of the river Daugava bed as one of the negative environmental off-site effects of soil erosion. Keywords: environmental impact, soil erosion, GIS modelling.

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L-13 ENANTIOSELECTIVE DETERMINATION OF ESSENTIAL α-AMINO ACIDS IN FERTILIZERS BY HPLC-MS/MS L. Taujenis, V. Olšauskaitė, A. Padarauskas Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania, [email protected]

Amino acids can serve as growth stimulants as they are fundamental ingredients in proteins biosynthesis and have strong influence in plants physiological activity [1]. Though only L-amino acids can be effectively assimilated by plants whereas D-amino acids are not involved in enzymatic growth processes or might be even toxic in some cases [2]. Therefore it is reasonable to control fertilizers in terms of chiral amino acids composition. Up to this date there are few papers which provide amino acid analysis method in fertilizer samples [3], although neither of them fully differentiates all essential amino acids enantiomers. In this study enantioselective high performance liquid chromatography – tandem mass spectrometry method was developed for 36 D- and L-αamino acids and glycine determination in fertilizer samples. High chiral resolution ranging from 0.83 for D/L-glutamic acid to 5.25 for D/L-proline was achieved on teicoplanin based Chirobiotic T2 chiral stationary phase. Triple quadrupole tandem mass spectrometer operating in multi reaction monitoring mode with ESI ion source was equipped for detection. Method was validated in terms of precision and accuracy. Finally, developed method was applied for D-/L-α-amino acids determination on five commercially available fertilizers. References [1] [2] [3]

M. Jie, W. Raza, Y.C. Xu, Q.-R. Shen, Journal of Plant Nutrition 31 (2008) 571. H. Svennerstam, U. Ganeteg, O. Forsum, M. Zetherstrom, T. Nasholm, Amino Acids 33 (2007) Xxxvii. M.I. Alarcón-Flores, R. Romero-González, A.G. Frenich, J.L. Martínez Vidal, R.C. Reyes, Analytical Methods 2 (2010) 1745.

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L-14 SIMPLE UPLC-MS-TOF METHOD FOR NUCLEOTIDE POOL ANALYSIS IN BAKER’S YEAST CELLS (Saccharomyces cerevisiae) Marta Baumane1, Janis Liepins2, Juris Ķibilds2, Agnese Kokina2, Ilva Nakurte1 1

Faculty of Chemistry, University of Latvia, 48 Kr. Valdemara Str, Riga, [email protected] Institute of Microbiology and Biotechnology, University of Latvia, 4 Kronvalda Blvd., Riga, [email protected] 2

Baker’s yeast Saccharomyces cerevisiae shares similar chemical and structural features with higher eukaryotes. Therefore S. cerevisiae is often used as a simple eukaryotic cell model in biochemistry research. Nucleotides and their derivatives are crucial components of the living cell. They play central roles in metabolism; participate in cell signaling, and are incorporated into important cofactors of enzymatic reactions. Adenine nucleotides and oxidized and reduced pyridine coenzymes are of particular interest, since they are part of DNA, RNA and play central role in intracellular signaling and cell’s energy and redox metabolism states. Energy charge is defined as adenosine tri- and di-phosphate ratio to the sum of all adenosine nucleotides species. The aim of this study was to adapt a method for isolation and determination of nucleotides and their derivatives in yeast S. cerevisiae cell extracts using liquid chromatography – time of flight mass spectrometry. Nucleotides were separated on an Agilent 1290 series UPLC system, connected with an Agilent 6230 TOF LC/MS (Agilent Technologies, Germany) mass spectrometer. Elution solvents consisted of acetonitrile and 10 mM ammonium formate in water, using a 14 min gradient at a flow rate 0.290 ml min-1. LC separations were achieved by using an Atlantis dC18 (Waters) column 2.1× 150 mm, 3.0 μm. One full mass spectrum was acquired in profile mode, with mass range from m/z 50 to 1000. The data of nine following compounds – AMP, ADP, ATP, ADE, cAMP, NAD and NADH were obtained using the extraction of individual compound chromatogram, at their individual m/z values. Successful application of the method to a preliminary study of nucleotides in yeast S. cerevisiae cell extracts that display a “biochemical snapshot” of cell’s energy state illustrated the usefulness of the method.

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L-15 PHYSIOLOGICAL AND BIOCHEMICAL MECHANISMS OF PLANT ADAPTATION TO THE STRESS ACTION OF HEAVY METALS: IMPLICATIONS FOR THE PHYTOREMEDIATION TECHNOLOGY Massimo Zacchini, Fabrizio Pietrini, Valentina Iori, Daniela Di Baccio, Angelo Massacci Institute of Agro-environmental and Forest Biology (IBAF) CNR - Research Area of Rome1, Via Salaria Km. 29,300 - 00015 Monterotondo (Rome) Italy Heavy metal exposure affects several morphological and physiological processes in plants, altering water balance, nitrogen metabolic activities, and nutrient uptake and distribution. In particular, heavy metals can interact directly or indirectly with the photosynthetic machinery causing a reducing metabolic capacity for CO2 fixation. Heavy metals can also induce the production of reactive oxygen species (ROS) that can damage cell components such as nucleic acids, proteins and polysaccharides modifying the cellular redox state. The excess of metal accumulation in plant tissues can result in severe stress symptoms such as chlorosis, necrosis, decrease in water potential and transpiration, inhibition of growth and death. To face the heavy metal presence in water and soil, plants have evolved a wide array of morphological, physiological and biochemical modifications allowing them to avoid or limit metal absorption or, in contrast, to accumulate and safely store the metals at cellular and tissue levels. Such processes represent the basic properties to be exploited for the utilisation of plants in the metal decontamination of soil and water through the bio-technology called phytoremediation. To be really effective and widely applied, phytoremediation needs the availability of plant species, clones and ecotypes able to grow in many different environmental conditions. In order to improve the ability of plants to survive and perform the metal remediation of soil and water, a genotype selection for superior performances in metal tolerance and accumulation is required. Thus, the characterisation of the morpho-physiological and bio-molecular traits involved in these processes represents a key point in order to assist the selection of plants for phytoremediation.

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L-16 THE EFFECTS OF NATURAL ANTIOXIDANTS COMPLICATIONS IN PATIENTS WITH TYPE 2 DIABETES

ON

PROCESS

OF

1

NIJOLE SAVICKIENE, 2ASTA SPADIENE, 3ANDREJS SKESTERS, 3ALISE SILOVA, 4 ANTANAS NORKUS, 4RYTAS OSTRAUSKAS, 5MIGLE FRANCAITE – DAUGELIENE 1

Department of Pharmacognosy, Medical Academy of Lithuanian University of Health Sciences, Lithuania Department of Drug Chemistry, Medical Academy of Lithuanian University of Health Sciences, Lithuania 3 Laboratory of Biochemistry, Riga Stradins University, Latvia 4 Endocrinological Institute, Medical Academy of Lithuanian University of Health Sciences, Lithuania 5 Endocrinological Clinic, Hospital of Lithuanian University of Health Sciences Kaunas Clinics 2

In this study (International “Eureka“ project E! 3695 „Creation of the methodology for effects of natural antioxidants on the development of the Diabetes mellitus complications“) we evaluated the effects of Ginkgo biloba L. and Camellia sinensis L. leaves extracts on oxidative damage in type 2 diabetic patients with surrounding diabetic complications (neuropathy, nephropathy, rethinophaty) [1]. All subjects (88 patients) were outpatients of the Endocrinology Clinic, Hospital of Lithuanian University of Health Sciences Kaunas Clinics. Subjects diagnosed with type 2 diabetes mellitus (treated with Insulinum, Metforminum or combination of both), aged from 35 to 80 years old and followed up for diabetic retinopathy, nefropathy or neuropathy were enrolled into the study. All patients were randomly allocated to receive standardized Ginkgo biloba dry extract (Ex.Gb), Ex.Cs (standardized Camellia sinensis L. leaves extract), compound of both extract - Grinvitals Cereloba plus tablets (GCP) or placebo capsules. The baseline measurements were repeated after 9 and 18 months of receiving preparations. First nine months patients received capsules twice a day, second nine months – three times a day. Placebo capsules were made from microcrystalline cellulose, a material indifferent to disease. The comparisons between three different measurements (at baseline, after 9 and 18 months) were made using Friedman test and Two-way ANOVA. The results were considered statistically significant at p < 0.05. The results confirm the beneficial influence of Ex.Gb on reducing amount of MDA (malondialdehyde) and HNE (4-hydroxy-2-nonenal) in diabetic patients. The strongest effect of Ex.Gb was observed on the amount of lipid peroxidation markers. While the Ex.Cs didn’t have significant effects. Ex.Gb had the most influence on perivascular changes (perivascular intumescence, haemorrhages, accumulation of hemosiderin, lipid infiltration of conjunctiva) in bulbar conjunctiva vessels. The results show that ingestion of Ex.Gb (240 mg per day) determines significant decrease in quantity of creatine. It was estimated the minor improvement of indicators in psychological status and quality of patients life after the application of Ex.Gb (160 mg and 240 mg per day) – for women and Ex.Cs ( 400 mg and 600 mg per day) – for men. The results of this study show that ingestion of antioxidants (ExGb, ExCs) has possibility to determine the significant positive changes of complications [2]. 1. Lu JM, Lin PH, Yao Q, Chen C (2010) J Cell Mol Med 14(4): 840-860. 2. Barnes J, Anderson LA, Phillipson JD (2002) Herbal Medicines Second edition.Pharmaceutical Press, New York.

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APPLICATIONS Saulius Kaciulis Institute for the Study of Nanostructured Materials, CNR of Italy The importance of surface analysis is constantly increasing due to the enhancement of research topics dedicated to the nanotechnology and nanostructured materials for the use in the fields of microelectronics, anti-corrosive and anti-wear coatings, biomedicine, biotechnology, gas sensing, etc. Constantly shrinking dimensions of the devices and increasing role of the processes, taking place on the materials surface, are defining the increasing demand for surface characterization of new materials. The present lecture is dedicated to the principles and applications of the mostly diffused techniques of surface analysis: electron spectroscopies XPS and AES. X-ray Photoelectron Spectroscopy (XPS), also known as Electron Spectroscopy for Chemical Analysis (ESCA), is used to determine quantitative atomic composition and chemistry. Its sampling volume extends from the surface to a depth of approximately 5 - 10 nm. Alternatively, XPS can be utilized for sputter depth profiling to characterize thin films and coatings by quantifying chemical species as a function of depth. XPS as analytical technique is unique in providing chemical state information of the detected elements. Auger Electron Spectroscopy (AES) is a surface-specific technique that utilizes a high- energy electron beam as an excitation source. Atoms excited by the electron beam can relax through the emission of Auger electrons with kinetic energies, which are characteristic of elements present at the sample surface. AES can be successfully employed for sputter depth profiling and for high resolution chemical imaging of conductive materials. Numerous examples of practical application of XPS and AES for the characterization of different materials (thin films, anti corrosive coatings, allotropes of carbon, supramolecular assemblies, nanoparticles, enzymes, etc.) will be disclosed during this lecture.

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L-18 ANALYSIS OF BEE PRODUCTS USING CHROMATOGRAPHIC TECHNIQUES: A REVIEW Vilma Kaškonienė Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania Bee products are very valuable for consumers due to their unique properties, which cover the “power” of plants and bees itself. Bee products are used as food or are introduced in the folk medicine from the ancient times. Honey is the most favourable out of all bee products due to it sweet taste and characteristic aroma. Bee bread, bee pollen, propolis, bee wax, royal jelly and bee venom are also very valuable bee products with a wide spectrum of biological activity. The latter two are not so easily available for the consumers and mainly are used in pharmacy products. However the use of bee products as food or as medicine, requires determination of their composition, evaluation of quality and biological activities or verifying the authenticity. The variety of chromatographic techniques allows to evaluate many parameters concerning to the bee products composition or impurities. Sugar composition can be determined by different methods, e.g. HPLC with refractometric detection, HPLC with pulsed amperometric detection, ion exchange chromatography with pulsed amperometric detection. GC-FID and GC-MS also can be applied for sugar analysis; just in this case the derivatization of carbohydrates must be performed to make them volatile. Aroma of bee product can be analyzed by GC with various detectors, after different sample preparation techniques, i.e. direct headspace analysis, solid phase microextraction (SPME), solvent extraction (SE), Likens-Nickerson, purge-and-trap or hydrodistillation. The residues of pesticides, fungicides and antibiotics in bee products can be determined using HPLC or GC after a proper sample preparation. The SPME, solid phase extraction, supercritical fluid extraction, SE, stir bar sorptive extraction, and accelerated solvent extraction can be used for the sample preparation. HPLC, nano-liquid chromatography, capillary electrophoresis and SPME-GC can be used for the analysis of the phenolic compounds profile and quantitative evaluation. The HPLC with electrochemical or reaction detectors can be employed for the evaluation of antioxidant activity of bee products. Key words: bee products, sample preparation, HPLC, GC-MS.

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L-19 CONTACTLESS ELECTROPHORESIS

CAPACITIVE

DETECTOR

FOR

CAPILLARY

Tomas Drevinskas, Audrius Maruška Vytautas Magnus University, Faculty of Natural Sciences, Dept. of Biochemistry and Biotechnologies, Vileikos 8, LT44404 Kaunas

Capacitance-to-digital conversion technology is a promising technique for chemical analysis. The monitoring of change of capacitance due to presence or lack of certain compound can be applied for volatile compound detection. In present work detector capable of detecting charged analytes in liquid during process of separation is described. Total system can be operated from the battery and is portable. Electrophoretically Separated metal cations can be detected down to sub-micro-molar concentrations. The work describes hardware and design issues and benefits.

Acknowledgement. The research work was financed by the Research fund of Vytautas Magnus University, grant No. P-BF-13-07.

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L-20 Sustainability: Balancing Scientific (Chemical) Progress and Risk Hartmut Frank Environmental Chemistry & Ecotoxicology, Bayreuth Center of Ecology and Environmental Research, University of Bayreuth, Hugo-Rüdel Str. 8, D-95445 Bayreuth, Germany ([email protected]) Cultural progress is to a great extent driven by advances in scientific (chemical-analytical) progress and the ensuing technical abilities, but also upon a number of other factors, such as the conditions and interests of the various societal stakeholders. If these latter aspects are not appropriately considered, the outcome of environmental-scientific investigations can become inefficient (Sarewitz 2004) or even grossly unfair (Shrader-Frechette 2010). Such risk does not only exist in dictatorial forms of governments but also in democratic societies consisting of more and less powerful fractions. In principle, this is a matter of ethics (Frank 2013). Therefore, before scientific investigations of given environmental problems or phenomena are planned and the actual technical performance of given research projects are conceived, such ethical and social aspects should be considered, inasmuch as the outcomes and interpretation of experimental eco/toxicological investigations have their intrinsic inaccuracies, involve the contributions from diverse fields of natural sciences, and depend upon the definition of error margins (Moser and Dondi 2012). In this lecture, the complexities of such procedures are discussed, taking as examples the risks associated with modern pesticide or pharmaceutical production and use, or of the impact of airborne volatile organic components on the forest decline phenomenon. Most importantly, for fair environmental policy decision making, the concept of a new paradigm for academic education in science is needed in which the ethical relevance of its applications is also considered (Frank et al 2013; McGregor and Wetmore 2009; Morin 1999). Frank, H. 2013. Environmental science, culture, and ethics. Toxicological & Environmental Chemistry 95: 13 Frank, H., L. Campanella, F. Dondi, J. Mehlich, E. Leitner, G. Rossi, K. Ndjoko Ioset, and G. Bringmann. 2011. Ethics, chemistry, and education for sustainability. Angewandte Chemie International Edition 50: 8482 – 8490 McGregor, and J.M. Wetmore. 2009. Researching and teaching the ethics and social implications of emerging technologies in the laboratory. Nanoethics 3: 17-30 Morin, E. 1999. Les sept savoirs nécessaires à l’éducation du future. Paris, UNESCO Publishing. English version: Seven complex lessons in education for the future. Available at http://www.unesco.org/education/tlsf/mods/theme_a/img/03_sevenlessons.pdf Moser, F., and Dondi, F. 2012. On the road to RioY20: The evolution of environmental ethics for a safer world. Toxicological & Environmental Chemistry 94: 807-813 Restrepo, G., M. Weckert, R. Brüggemann, S. Gerstmann, and H. Frank. 2008. Ranking of refrigerants. Environmental Science & Technology 42: 2925-2930. Sarewitz, D. 2004. How science makes environmental controversies worse. Environmental Science & Policy 7: 385–403 Shrader-Frechette, K. 2010. Analyzing public participation in risk analysis: How the wolves of environmental injustice hide in the sheep’s clothing of science. Environmental Justice 3: 119-123

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L-21 CELLULOSE DERIVATIVES: SYNTHESIS AND APPLICATION

Jolanta Liesiene Kaunas University of Technology, Department of Polymer Chemistry and Technology, Kaunas, Lithuania, [email protected]

Cellulose, a linear 1,4-β-glucan, is the most abundant polymer worldwide. Cellulose as a raw material is used for two general purposes: (i) natural textile fibers, and (ii) paper and board. Although native cellulose is hydrophilic, it is insoluble in water or popular organic solvents. With the aim to widen its application areas, cellulose derivatives are prepared. In this presentation short history of cellulose chemistry is introduced. New trends in cellulose chemistry and physics are discussed. Synthesis of new soluble cationic and amphiphilic cellulose derivatives and their application for drug delivery, coating of the silica surface as well as for preparation of coated chromatographic stationary phases are presented. The formation of a macroporous cellulose gel and its application for different purposes is reported. The preparation of cellulose-based adsorbents for protein chromatography and for enzyme immobilization is presented. The sorption of bile acids on cellulose-based adsorbents is discussed.

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P-1 GENETIC POLYMORPHISMS OF MITOCHONDRIAL GENOME D-LOOP REGION IN COMMON MERGANSER (MERGUS MERGANSER) OF LITHUANIA Auksė Abromaitytė1, Aniolas Sruoga1,2, Algimantas Paulauskas1, Vaida Tubelytė1, Vykintas Baublys1 1

Department of Biology, Vytautas Magnus University, Vileikos 8, Kaunas, LT 44404, Lithuania. Email address: [email protected] 2 Institute of Ecology, Nature Research Centre, Akademijos 2, LT 08412 Vilnius, Lithuania Common merganser (Mergus merganser) is a member of Anatidae family and Merginae subfamily. Now they are found all over Lithuania country. Despite this fact species was very rare few decades ago in Lithuania and at this moment is written into The Red List of Lithuania fauna at 5(Rs) category. Till now, there are no researches done on the Common merganser population structure in Lithuania using molecular methods. Essential and significant aim is to explain and determine the origination of the Common mergansers recovered population in Lithuania and to detect genetic variability of breeding mergansers in Lithuania. Genetic variability investigation can lead to evaluation of Lithuanian common merganser fitness. The aim of our research is to analyze the population structure of Common merganser by investigating mitochondrial DNA D-loop control region of migrating and breeding Common mergansers samples from Lithuania. The material for molecular research was collected in collaboration with the Institute of Ecology of Vilnius University. For molecular analysis samples were taken from 40 Common merganser blood, heart and liver samples. The material was collected in 2003-2014 from the Baltic Sea coastal zone, Kaunas, and Vilnius regions. Amplification of a 514 bp fragment of the mtDNA D-loop region was performed using MMCRL primer. Amplified PCR products were sent for sequencing. Received data were analyzed by MEGA 6 software package, and data were processed by statistical computer programs. Data were compared with the material of other researchers acquired by the same marker and compared with individuals from other countries in Europe, Asian part of Russia and North America. Among other individuals the unique haplotypes were found, that hasn‘t yet been detected by other scientists in Common mergansers. Total of 6 different haplotypes were found among the samples from Lithuania. Two unique haplotypes were found in Lithuanian breeding population. The parameters of genetic structure of Common merganser was evaluated, such as frequency of mtDNA haplotypes. Pairwise comparison between Lithuania’s migrants and breeding individuals was nonsignificant (average pair distance was 0,005). Also we compared our haplotypes with the ones that were already acquired by other scientists. The results raised the hypothesis that population of Lithuania was re-established from breed migrants from Scandinavia.

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P-2 COMPARISON OF LIGHT-EMITTING DIODES AND HIGH-PRESSURE SODIUM LAMPS LIGHTING EFFECTS ON TOMATO TRANSPLANTS Aistė Bagdonavičienė, Aušra Brazaitytė, Julė Jankauskienė, Sandra Sakalauskienė, Giedrė Samuolienė, Akvilė Viršilė, Jurga Miliauskienė, Ramūnas Sirtautas, Pavelas Duchovskis

Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas str. 30, LT-54333 Babtai, Kaunas distr., Lithuania The objective of our studies was to compare the growth of tomato transplants, cultivated under illumination of the high-pressure sodium (HPS) and light-emitting diodes lamps, and intentionally designed to replace HPS lamps. The transplants of tomato hybrid ‘Magnus F1’ were grown in phytotron chambers. Day/night temperature till germination was 22-18°C, and 16 h photoperiod was maintained. A solid-state lighting lamp containing 665 and 451 nm light-emitting diodes (LEDs) was used in the experiments. Total photosynthetic photon flux density was 200 µmol cm-2s-1. For comparison, tomato transplants were grown under the illumination of high-pressure sodium lamps (HPS). Our investigations revealed that under irradiation of red 665 nm and blue 451 nm lightemitting diodes tomato transplants were smaller, had smaller leaf area, number of leaves, hypocotyl diameter and length and fresh and dry weight as compared to HPS lamps. Illumination with LEDs lamps caused the decrease of chlorophyll a and b content, but determined higher chlorophyll a and b ratio and carotenoids contents in their leaves. Their development has been slower as compared to transplants cultivated under high-pressure sodium (HPS) lamps. Our study showed, that broader spectrum high pressure sodium lamps are more suitable for high-quality tomato transplant cultivation in closed growth chambers, as compared to red 665 nm and blue 451 nm light-emitting diodes lighting. These lamps may be more use full for supplemental lighting in greenhouse conditions where total lighting flux contains background natural lighting. Therefore, these studies will be continued. Key words: high-pressure sodium lamps, growth, photosynthetic pigments, light-emitting diodes, tomato, transplants

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P-3 DETECTION OF CANINE BABESIOSIS CAUSATIVE AGENT BABESIA CANIS INDETRMACENTOR RETICULATUS TICKS IN LITHUANIA AND CENTRAL EUROPE Banaitytė Asta, Radzijevskaja Jana, Paulauskas Algimantas Faculty of Natural Sciences, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas, Lithuania Dermacentor reticulatus ticks are considered as an important vector of different viruses, bacteria, protozoa, which causes a variety of diseases in animals and humans. D. reticulatus is the most important vector of Babesia canis protozoan. B. canis, also known as large canine Babesia, can cause severe disease in infected dogs. The most frequent causative agent of canine babesiosis in Central Europe is B. canis canis. Recently, many new endemic foci of this disease have been reported from European countries. Growing incidence of canine babesiosis was recorded also in Lithuania during the last decade. Information on prevalence of babesia pathogens is scarce, especially in Lithuania, where the large-scale investigation of babesia pathogen in D. reticulatus tick never has been done before. The aim of our study was to investigate the prevalence of B. canis in D. reticulatus ticks collected in 34 locations in Lithuania, Poland, Slovakia, Latvia and Ukraine by using molecular methods. A total of 1331 ticks were collected during April – May of 2013, and examined for the presence of Babesia DNA. For pathogen detection, nested PCR with external (BTF1, BTR1) and internal (BTF2, BTR2) primers, which amplify partial region of the 18S rRNA gene of Babesia species, was carried out. In total 0.95 % of all tested samples were positive for B. canis. In Lithuania prevalence of B.canis ranged from 0 to 6.7% in different locations. In Poland, B.canis pathogens were detected with prevalence ranged in different locations from 0% to 2.7%. No positive samples were found in investigated locations of Latvia, Slovakia and Ukraina. Sequence analysis showed that all Babesia isolates from ticks belonged to B. canis canis subspecies. Analysed 18S rRNA gene sequences were 98–100% similar to the sequences of B. c. canis isolated from ticks in other European countries. Key words: Dermacentor reticulatus, Babesia canis canis

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P-4 THE EFFECT OF LIGHT SPECTRUM COMPOSITION ON THE METABOLITE CONTENT IN LETTUCE AND BEET Alina Čeidaitė, Giedrė Samuolienė, Pavelas Duchovskis, Aušra Brazaitytė, Akvilė Viršilė Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas str. 30, LT-54333 Babtai, Kaunas distr., Lithuania Metabolites, such as antioxidants (ascorbic acid, total phenolics, total anthocyanins and DPPH freeradical scavenging capacity) neutralize free radicals and make positive effect on human health. The aim of this study was to evaluate the effect of light-emitting diode (LED) spectrum composition on the antioxidant content in young vegetables. Young vegetables of beet (Beta vulgaris L., ‘Bulls Blood’) and lettuce (Lactuca sativa L. ‘Multigreen3’ and ‘Multired4’) were investigated. The experiments were carried out in phytotron chambers. The composition of LED modules were as follows: 455 (blue), 638 (red), 660 (red), 735 (far red) nm (basal); basal + 595 nm (yellow); basal + 622 nm (orange), basal + 520 nm (green). The photosynthetic photon flux density (PPFD) of each module was about 300 µmol m-2 s-1. The photoperiod of 18 hours and 21/16 ºC day/night temperature in the phytotron chamber were maintained. According to our results, the response of particular vegetable to light quality was different. But in general, better antioxidant properties of tested vegetables were under basal or basal supplemented with green LEDs. Keywords: metabolite, antioxidant, anthocyanin, phenols, ascorbic acid, DPPH

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P-5 THE RESPONSES OF GAS EXCHANGE PARAMETERS AND LEAF CHLOROPHYLL CONTENT TO ELEVATED CO2 AND DIFERENT SOIL N AVAILABILITY IN PISUM SATIVUM LEAVES Austra Dikšaitytė, Irena Januškaitienė Vytautas Magnus University, Faculty of Natural sciences, Department of Environmental sciences, Vileikos 8, Kaunas, LT-44404, Lithuania As both increasing atmospheric carbon dioxide and soil nitrogen play a pivotal role in plant growh and metabolism, there is in particular interest to study their interaction on plant performance, especially, when nitrogen, both extra N application and N deficiency, may play a role in the response of plant to elevated CO2. In order to study their interaction on photosynthetic and growh responses of plant, growth chamber experiments were performed with pea (Pisum sativum L.) plants grown in a controlled conditions at ambient (400 ppm) or elevated (700 ppm) CO2 levels under different soil N availability (3 and 12 g m-2 of nitrogen). The measurements of photosynthetic indices, chlorophyll content and dry total plant mass were performed at the end of an experiment after 10-days duration of treatment. The results showed that photosynthetic characteristics and growth of pea were significant affected by both elevated CO2 and nitrogen. At both N levels, leaves of pea plants grown under elevated CO2 had significant higher photosynthetic rate, lower stomatal conductance (gs), higher water-use-efficiency (WUE) and total chlorophyll content. The total dry mass of pea was significant higher at elevated CO2 by 41 and 35,2 % under 3 and 12 g m-2 of nitrogen in the soil, respectively. Although the gs reduced in elevated CO2, this by itself does not limited photosynthesis, because the ratio of intercellular to ambient CO2 concentration (ci/ca) at both nitrogen levels was higher under elevated than ambient CO2. The photosynthetic indices mostly differed significantly between two nitrogen levels with the lower values of photosynthetic rate and WUE and higer gs and ci/ca under nitrogen deficient at both CO2 concentrations, but the total dry mass and chlorophyll content do not doing so. Nevertheless, for all these photosynthetic and growth responses were detected significant CO2, N and CO2 × N interaction, who revealed the dependence of the CO2 response on N availability in the soil. Moreover, because the impact of increased CO2 concentration, promoting photosynthetic rate and growth of pea plants, decreased at higher N level, compared with low N availability, this may by indicate increased nitrogen-useefficiency in nitrogen-deprived pea plants. So, it is concluded that the levels of soil nitrogen fertilization will play a great role in modulating the CO2 fertilization effect on pea plants under future elevated CO2 and nitrogen condition, but in the absence of other environmental stresses, at elevated CO2 pea proved to be perform well even under nitrogen deficiency. Keywords: pea, elevated CO2, nitrogen, photosynthetic rate, WUE, growth

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P-6 THE EFFECTS OF GINKGO BILOBA L. AND CAMELLIA SINENSIS L. EXTRACTS ON DIABETES-RELATED DISTRESS AND QUALITY OF LIFE IN PATIENTS WITH TYPE 2 DIABETES A. Špadienė1, L. Lašaitė2, N. Savickienė3, H. Rodovičius1, G. Kazanavičius4 1

Department of Drug Chemistry, Faculty of Pharmacy, Lithuanian University of Health Sciences, 2 Institute of Endocrinology, Lithuanian University of Health Sciences, 3 Department of Pharmacognosy, Faculty of Pharmacy, LithuanianUuniversity of Health Science, 4VIA MEDICA, Kaunas

Diabetes (DM) has strong negative impact on quality of life (QoL). People with diabetes feel overwhelmed by the continuous burden of their disease and its management. These emotional and social burdens may be compounded by the acute physical distress of hyperglycemia and by the chronic physical distress of diabetesrelated complications. On the other hand, these persistent feelings diminishes self-care, which in turn leads to worsened glycemic control, increased risk for complications, and exacerbation of diabetes. Thus,it is also very important to have good QoL maintaining long-term health and well-being. The aim of this study was to evaluate possible changes in psychological sate of type 2 diabetic patients treated with Green tea and Ginkgo biloba extracts, acting as antioxidants in development of DM.

The results are from 49 patients with type 2 DM (aged 35-80) which were randomly allocated to receive standardized Ginkgo biloba (Ginkgo biloba L.) dry extract (Ex.Gb), standardized Green tea (Camellia sinensis L.) leaves extract (Ex.Cs) or placebo capsules. All participants were asked to fill in two self-administred questionnaires at baseline and after 9 and 18 months of receiving preparations. WHO Brief Quality of Life Questionnaire was used to assess the QoL [1]. Diabetes Distress Scale was used to assess psychological distress related to DM [2]. There were no statistically significant differences neither between men nor women in Ex.Gb and Ex.Cs groups as compared with placebo group. However, some significant changes were detected within both antioxidant groups. Interpersonal distress related to DM significantly decreased (p=0,045) and environment aspect of QoL significantly improved (p=0.05) in women of Ex.Gb group after 9 and after 18 months. As well as environment aspect of QoL significantly improved (p=0.017) in men of Ex.Cs group. Meanwhile, there were no significant changes during all the period of the study in placebo group, neither between men nor women. In conclusion, Green tea and Gingko biloba extracts with strong antioxidant effect have positive impact on psychological state in patients with type 2 diabetes mellitus. Nevertheless, the further study in different circumstances needs to be performed. This research is a part of international "Eureka“ project „E!3695” „Creation of the methodology for effects of natural antioxidants on the development of the Diabetes mellitus complications“ financially supported by the European Social Fund Agency. 1. 2.

WHO (1996) WHOQOL-BREF: introduction, administration, scoring and generic version of the assessment. World Health Organization. Field Trail Version Fisher L, Glasgow RE, Mullan JT, Skaff MM, Polonsky WH (2008) Development of a brief diabetes distress screening instrument. Annals of Family Medicine 6(3):246-252

P-7 SMALL MAMMALIAN SUCCESSION AFTER FIRE IN PINUS BRUTIA (TEN.) FORESTS

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Kaynas, Burcin Y.1, and Gurkan, Behzat2 1

Mehmet Akif Ersoy University, Faculty of Arts and Science, Department of Biology, Burdur, TURKEY,2Yasar University, Faculty of Science and Letter, İzmir, TURKEY

In Mediterranean ecosystems, numerous fires occur and very large areas are affected by fires every year. Pinus brutia forests which have widespread distribution in eastern Mediterranean region are fire-resilient with their adaptive features that make them regenerate in a short period. In this study, we aimed to determine changes of small mammal community structure with successional gradient after fire in P. brutia forest. Sampling was made in three replication sites of every successional stage (burned 3, 6, 9, 10, 16 and 26 years ago) and two for unburned areas. Sherman live traps were used to capture small mammals in 5x5 grid with 10 m intervals in every replication site. Microhabitat variables were recorded around the all trap locations to determine habitat preferences of small mammal species. Total 675 individuals belong to 6 small mammal species (Apodemus mystacinus, Apodemus flavicollis, Mus macedonicus, Crocidura suaveolens , Rattus rattus and Dryomys nitedula) were caught during the study. A. mystacinus is a species which prefers rocky and bare habitats was predominant all sites except one the replication site of the unburned areas. Abundances of A. flavicollis and C. suaveolens display increase with successional gradient and it was found that both prefer habitats which have higher vegetation height, cover and plant species richness. However, M. macedonicus which its abundance negatively correlated with plant species richness, shrub cover and shrub species richness, decrease with the successional gradient. As a result, small mammals showed a change with successional gradient. The changes of small mammal community structure occurred as depend on habitat components relating to plant architectural and vertical diversity.

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P-8 DISTRIBUTION OF DERMACENTOR RETICULATUS TICKS IN LITHUANIA Mardosaitė Dalytė, Radzijevskaja Jana, Paulauskas Algimantas Faculty of Natural Sciences, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas, Lithuania

Ornate meadow tick, Dermacentor reticulatus (Fabricius, 1794) is important tick-borne disease vector in Europe. According to some recent observations, in Europe, increasing range of geographical distribution of D. reticulatus is observed. Lithuania is the northern boundary of the distribution of D. reticulatus in central Europe. However, limited information is available on the change of geographical distribution and habitat of D. reticulatus in Lithuania. Ticks were collected by flagging in spring and autumn in 77 locations in Lithuania in 2013-2014 year. Tick sampling was conducted on meadows, in river valleys, in mixed and deciduous forest, and ecotones between forested and grassy areas. Ticks were collected in daytime between 8.00 and 19.00 h by one - three persons at each site. A total of 2571 adult unfed D. reticulatus ticks have been collected in 63 sites located in twenty two districts. According to our observations D. reticulatus ticks were found sporadically in all part of country, except of locations in Samogitian uplands situated in north-western part of Lithuania. Relative tick abundance was estimated by determining the average number of ticks of each stage per person per 30 min. Ticks were found most regularly and in greatest numbers in open areas close to water basin and mixed forest. In the present investigation new areas of D. reticulatus occurrence in Latvia, by 30 km further north from northern Lithuanian border, were detected. This study providing evidence that D. reticulatus has extended its range in the surroundings of its former habitats in Lithuania. The study was partially supported by the Research Council of Lithuania (grant no. MIP-053/2013).

Key words: ticks, distribution, Dermacentor reticulatus, Lithuania

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P-9 HELMINTHS OF MUSTELIDS (MUSTELIDAE) IN LITHUANIA Dovilė Nugaraitė*, Vytautas Mažeika, Algimantas Paulauskas Vytautas Magnus University, Department of Biology, Vileikos str. 8, Kaunas LT-44404, Lithuania, *Corresponding author. E-mail: [email protected]

Mustelids (Mustelidae) the largest family in the order Carnivora. In Lithuania 9 species of these mammals are found. Parasites of mustelids have been studied in Lithuania about 40 years ago [1-2]. The objective of this study was to explore the helminthes of mustelids. The material was collected in different parts of Lithuania, in the period 2011–2014. This report is the result of a total helminthological dissection of individual organs of 25 mustelids: 2 pine martens (Martes martes), 4 stone martens (Martes foina), 10 European polecats (Mustela putorius), and 9 American minks (Neovison vison). A total 9 taxa of the parasitic worms were found: nematodes Crenosoma schachmatovae Kontrimavichus, 1969, Eucoleus aerophilus (Creplin, 1839), Aonchotheca putorii (Rudolphi, 1819), Molineus patens (Rudolphi, 1845), Nematoda g. sp., cestodes: Mesocestoides lineatus, Cestoda g. sp. and flukes Isthmiophora melis (Schrank, 1788) and Strigea strigis (Schrank, 1788) mesocercaria. I. melis in European polecat had the highest mean abundance (A = 391.3) and prevalence (P=90%) out of the all helminthes. I. melis also were found in American mink (A= 19.4, P=66%). Second species with high parameters of infection was Strigea strigis mesocercaria. In many cases the distribution of the helminthes among population of mustelids was aggregated (s²/A>1). The highest aggregation index was determined for I. melis (s²/A=635.5). References 1. 2.

Казлаускас Ю, Прусайте С (1976) Гельминты животных отряда хищных в Литве. Acta parasitologica Lituanica 14: 33–40 Senutaitė J, Grikienienė J (2001) Prevalence of Trichinella in muscles of some domestic and wild mammals in Lithuania and their impact on the organism. Acta Zoologica Lituanica 11: 395–404

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P-10 ANALYSIS OF ONCOGENE GLI1 PROTEIN EXPRESSION LEVELS BETWEEN DIFFERING GRADES OF ASTROCYTOMA Dovydas Gricius1,2, Arūnas Kazlauskas1 1

Laboratory of Neurooncology and Genetics, Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu str. 4, LT 50009 Kaunas, Lithuania 2 Faculty of Medicine, Lithuanian University of Health Sciences, Mickevičiaus str. 9, LT 44307 Kaunas, Lithuania Abstract Background: Aberrant expression of oncogene GLI1 has been linked to malignancies in many types of tissues including brain, pancreas, skin and breast[1-4]. It has been found that GLI1 expression is important for tumor progression and has been linked to tumor grade[1,5-7]. In this study, GLI1 expression has been analyzed in different malignancy grade astrocytomas. Materials and Methods: GLI1 protein expression was evaluated in 87 clinical tumor samples by using Western blot analysis. Associations between GLI1 expression and tumor grade were analyzed by applying the Mann-Whitney or Kruskal-Wallis tests. Kaplan-Meier survival analysis was performed to evaluate the prognosis of patients. Results: Western blot analysis did not show statistically significant correlation between GLI1 protein expression and astrocytoma tumor grade (P = 0.294). Kaplan-Meier survival also did not show correlation between survival rates and GLI1 expression (P = 0.081). Our results show that GLI1 protein expression levels in astrocytomas have higher variability than previously shown. Conclusion: Our results indicate that GLI1 protein expression is not an absolute requirement for the process of gliomagenesis. Yet, the relatively high (up to 52%) occurrence of GLI1 expression in astrocytomas suggest the need for further analysis on the involvement of GLI1-mediated Hedgehog signaling pathway in glioma tumorogenesis. References 1. Rossi M, et al. (2011) Cancer Biol Ther 11(8):753-761. 2. Nolan-Stevaux O, et al. (2009) Genes Dev 23(1):24-36. 3. Grachtchouk V, et al. (2003) EMBO J 22(11):2741-2751. 4. Cao X, et al. (2012) Oncogene 31(1):104-115. 5. Zhu H, et al. (2014) Cancer Lett 343(1):51-61. 6. Rush SZ, et al. (2010) Neuro Oncol 12(8):790-798. 7. Li Q, et al. (2011) ANZ J Surg 81(6):440-445.

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P-11 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY SEPERATION OF PSICHOACTIVES MEDICINS USING SOLID PHASE EXTRACTION FROM HUMAN URINE Daiva Kazlauskienė1 , Irina Halkevych2 1

– Lithuanian University of Health Sciences, Medical Academy, Faculty of Pharmacy, Department of Analytical and toxycological chemistry 2 – Danylo Halitsky Lviv National Medical University, Department of Toxycological and analytical chemistry The aim of this research – to develop the methodic for toxicological analysis buspirone, sertraline, quetiapine, venlafaxine, doxepine, paroxetine, bupropione and fluvoxamine mixture: SPE from human urine, identification techniques using HPLC in cases of intoxication with these drugs or a mixture of these drugs. Conclusions: 1. The SPE methodic was established: OAZIS cartridges preconditioned with 1 ml methanol and with 1 ml of distilled water. Then injected 2 ml urine sample. Washing with 2 ml solution of 25% ammonium hydroxide, methanol and distilled water (1:1:8). Next washing with 2 ml of methanol aqueous solution (1:9). The analyzed materials were eluted with 2 ml of methanol. Using these conditions 94-99 % of analyzed substances from the urine were extracted. 2. For identification HPLC method was used. A liquid chromatograph „Waters 2690“ and diodearray detector „Waters 996“ were used (230 nm). The is mixture of 0.1 % trifluorethane acid (A) and acetonitrile (B). Flow is gradient - 0 min.: A – 95 %, B – 5 %; 20 min.: A – 45 %, B – 55 %. (1 ml/min). The column ACE C18 (250 x 4.6 mm; 5 µm) was used. Using these conditions components in the mixture were completely separated (R ˃ 2). Keywords: extraction, SPE, HPLC, urine, antidepresants e:mail adress of coresponding author: [email protected]

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P-12 CONCENTRATION OF NITRATE IN Spinacia oleracea, Eruca sativa, Dipotaxsis sp., Lactuca sativa L., Lactuca sativa var. capitata, Brasicca rapa ssp. pekinensis Dovilė Laurinavičienė, Lina Trečiokaitė, Karolina Žalynaitė

The aim of the present study was to determine concentration of nitrate in Lithuanian and other leafy vegetables sold in the Lithuanian trade by ion chromatography method. Leafy vegetables samples were collected from different supermarkets and suppliers. For the analysis were taken samples of these vegetables: fresh spinach (Spinacia oleracea), conservated spinach, fresh lettuce (Lactuca sativa L.), cabbage lettuce (Lactuca sativa var. capitata), rucola (Eruca sativa, Dipotaxsis sp.), chinese cabbage (Brasicca rapa ssp. pekinensis). For nitrate concentration evaluation ion chromatograph Dionex ICS-1100 (USA) was used. The results showed that in all vegetables the content of nitrate differed considerably, independently from where and from what supplier leafy vegetables were bought. Concentration of nitrate did not exceeded maximum permitted level. The highest concentration of nitrate was found in rucola (1375,75 mg ∙ kg-1, SE=63,41) and lettuce (1270,54 mg ∙ kg-1, SE=40,71). The lowest concentration of nitrate was in Lithuanian spinach (143,78 mg ∙ kg-1, SE=14,61). Keywords: concentration of nitrate; leafy vegetables; Spinacia oleracea; Eruca sativa, Dipotaxsis sp.; Lactuca sativa L.; Lactuca sativa var. capitata; Brasicca rapa ssp. pekinensis; ion chromatography

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P-13 PRESENCE OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING E. COLI ISOLATES IN HEALTHY SHEEP* F. PEHLIVANOGLU, H. TURUTOGLU, D. OZTURK Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, 15030 Burdur, TURKEY By this preliminary study, presence of Extended Spectrum Beta Lactamase (ESBL) producing E. coli isolates was investigated in 100 fecal samples of sheep from randomly selected 6 herds in Burdur city of Turkey. One gram fecal sample was enriched in pepton buffered water for 24 hours and then 50 µl from enrichments were spread on chromogenic E. coli/coliform selective agar supplemented with 2 µg cefotaxime and 2 µg ceftazidime. After 24 hours incubation at 37 °C, one colony from each positive sample was identified as E. coli by conventional biochemical tests and E. coli spesific 16S rRNA gene determination by PCR. The ESBL positive E. coli isolates were determined by CLSI Confirmatory Test by using cefotaxime (30 µg)-cefotaxime +clavulanic acid (30 µg +10 µg) and ceftazidime (30 µg)-ceftazidime+clavulanic acid (30 µg +10 µg) discs. The results were evaluated according to CLSI criteria. Total 3 ESBL positive E. coli isolates were detected in sheep. In conclusion, we can state that existence of ESBL positive E. coli isolates in microbial flora of sheep is very low (3.0 %) in Burdur city of Turkey, but the presence of ESBL positive E. coli isolates should be investigated regularly to reveal any possible increase in prevalence in sheep since these isolates in microbial flora of intestines play important role for transfering resistance against to extended spectrum cephalosporins to pathogen microorganisms. *This study was supported by The Scientific and Technological Research Council of Turkey (Tübitak)(Project Number: 112 O 820)

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P-14 THE USE OF ACINETOBACTER SP. FOR OIL HYDROCARBONS DEGRADATION IN SALINE WATERS Elnara Fatajeva1, Artūras Juciūnas2, Dainius Paliulis3, Saulius Grigiškis4 1,3,4

Vilnius

Gediminas

technical

university,

1,2,4

Biocentras,

JSC

This paper describes the problem of oil pollutants removal and Acinetobacter genus microorganisms use in oil biodegradation. Microorganisms used in this research were obtained from JSC „Biocentras“ collection and during experimental procedure, the most efficient naphtha and fuel oil degrading Acinetobacter genus microorganisms were selected. Influence of environmental conditions (salinity, temperature, pH, substrate concentration) on their viability and biodegradation effectiveness were evaluated. The most efficient degradation of naphtha product by Acinetobacter genus bacteria was at 30° C temperature, in a pH range of 7-8. Marine condition imitating water salinity ranged from 3,5 ‰ to 35 ‰ had no significant effect on microorganism growth and effectiveness of naphtha biodegradation. Key words: Acinetobacter sp., oil hydrocarbons, degradation, saline water.

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P-15 STABLE CARBON ISOTOPE RATIO (δ13C) MEASUREMENT OF GRAPHITE USING EA-IRMS SYSTEM Edmundas Bružas 1, 2, Andrius Garbaras 2, Vidmantas Remeikis2 1

Kaunas University of Technology, Kaunas Lithuania

2

Center for Physical Sciences and Technology,Vilnius Lithuania

Graphite was and still is an important material in Magnox, AGR, HTR and RBMK type nuclear reactors [1] in which it is used as neutron moderator. For the management of used nuclear graphite the evaluation of neutron flux must be made [47]. One of the methods used for this purpose is isotope ratio mass spectrometry. Therefore there must be determined the best way of graphite sample preparation for the isotope ratio (δ13C) measurements. In this work δ13C values in not irradiated natural graphite were measured. The measurements were made using elemental analyzer combined with stable isotope ratio mass spectrometer (EA-IRMS). The samples were prepared with grinded and non-grinded graphite the part of which was mixed with Mg(ClO4)2. The best combustion of graphite in the oxidation furnace of the elemental analyzer was achieved when the amount of pulverized graphite ranged from 200 to 490 µg, and the ratio C:Mg(ClO4)2 was approximately 1:10. [1] Remeikis V, Plukis A, Plukienė R, Garbaras A, Barisevičiūtė R, Gudelis A, Gvozdaitė R, Duškesas G, Juodis L (2010) Nucl Eng Des 240:2697–2703.

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P-16 NEEDLE NITROGEN CONCENTRATION POPULATIONS OF JUNIPERUS COMMUNIS L.

OF

LITHUANIA

Edvina Krokaitė, Ramūnas Vilčinskas, Algimantas Paulauskas, Eugenija Kupčinskienė Department of Biology, Vytautas Magnus University, Vileikos str. 8, LT-44404 Kaunas, Lithuania

Juniperus communis L. is one of the most cosmopolitan conifer species that belongs to the family Cupressaceae. This species could be found in both the eastern and western hemispheres [1]. Juniper grows naturally in Lithuania and it is the third spontaneous conifer after pine and spruce. Till now biology of junipers did not received proper attention in Baltic States. Plants are very valuable and widely used in the food industry, medicine and manufacture of essential oils. The current application is usually pharmacological caused by essential oils, which are common for the plants of this genus. Concentrations of secondary metabolites are tightly related to plant nutrients. Elemental concentrations of junipers are not very widely analysed in both national and global levels but it is known that this plant is sensitive to changes of nitrogen at its growth environment. J. communis usually grows in poor according to nitrogen soils [2]. In addition, most studies concerning element concentrations were done without separation of the needles into current-year and one year old. This element is an essential component needed to thrive, used in photosynthesis as a rule limited in supply [3]. The main purpose of this investigation was to measure the concentration of nitrogen in current-year needles of Juniperus communis L. The material was collected from various locations of Lithuania, reflecting all geographical and habitat range. Later common juniper needles were turned into powder and nitrogen concentration was determined using the Kjeldhal method. Nitrogen concentration in the needles was below 2 % d. m. It demonstrates that main nutrient concentrations are low for juniper and resembles situation described for the other conifers of Lithuania. Key words: Kjeldahl method, nutrition, conifer, Cupressaceae. References 1. 2. 3.

Adams R. P. (2011) The Junipers of the World: The genus Juniperus, 3rd edition. Trafford Publishing, Victoria, BC. Hill M.O., Mountford J.O., Roy D.B., Bunce R.G.H. (1999) Ellenberg's Indicator Values for British Plants: Technical Annex. ECOFACT, Vol.2. Institute of Terrestrial Ecology, Huntingdon, UK. Galloway J.N., Dentener F.J., Capone D.G., Boyer E.W., Howarth R.W., Seitzinger S.P., Asner G.P., Cleveland C.C., Green P.A., Holland E.A., Karl D.M., Michaels A.F., Porter J.H., Townsend A.R., Vöosmarty C.J. (2004) Nitrogen cycles: past, present, and future. Biogeochemistry, Vol.70:153–226.

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P-17 SYNTHESIS OF NEW 4-IMIDAZOLINONE COMPOUNDS Eligijus Kupriūnas1, Jonas Salys1, Liudas Šlepikas1, Eduardas Tarasevičius2, Sigitas Tumkevičius3, Hiliaras Rodovičius1 1

Lithuanian University of Health Sciences, Faculty of Pharmacy, Department of Drug Chemistry, Eivenių 4, LT-50161 Kaunas, Lithuania 2 Vilnius University, Faculty of Medicine, Department of Pathology, Forensic Medicine and Pharmacology, M. K. Čiurlionio g. 21/27, Vilnius, Lithuania 3 Vilnius University, Faculty of Chemistry, Department of Organic Chemistry, Naugarduko 24, LT-03225 Vilnius, Lithuania

INTRODUCTION Recent data shows that 4-imidazolinones possessing a variety of biological effects such as anticonvulsant, anesthetic, antiarrhythmic, antiepileptic [1]. It also has been a favorable scaffold for developing novel anticancer compounds [2-3]. There is no data about new 4imidazolinone compounds that would have amino acids substituents at the 2 position in a 4imidazolinone ring. AIM To synthesize new 4-imidazolinone compounds, with substituents in 2 and 5 positions and optimize an amino acids nucleophilic substitution reaction in 2 position of 4-imidazolinone ring. METHODS Modifications of a 4-imidazolinone ring were performed in a 5 position by the Knoevenagel condensation reaction. To modify a 2 position a nucleophilic substitution reaction was performed with amino acids, previously incorporating methylthio functional group as a good leaving group. Different catalysts and solvents were used for reaction optimization. All reactions mixtures were refluxed for 48 h. Thin layer chromatography (TLC) was used to monitor the progress of reactions. Structures of compounds were determined using 1H NMR spectroscopy, IR spectroscopy and melting point determination. RESULTS Nucleophilic substitution reactions were performed in different solvents: ethanol, acetonitrile Different catalyst were used for reaction: potassium acetate, sodium acetate, sodium acetate hydrate, pyridine and DIEA (N,N-diisopropylethylamine). Reaction products obtained using catalysts potassium acetate and sodium acetate in solvents ethanol and acetonitrile. Small amount of product obtained using pyridine as a catalyst. Using DIEA final product found only in ethanol. No identifiable amount of product obtained using sodium acetate hydrate. New compounds structures were approved using 1H NMR and IR analysis methods. CONCLUSIONS. In this work we have synthesized new 4-imidazolinones compounds that have amino acids substituents in 2 position of the ring. Reaction conditions were optimized and new catalysts were founds for this reaction. REFERENCE 1. 2. 3.

Kieć-Kononowicz K, Stadnicka K, Mitka A, Pekala E, Filipek B, Sapa J, Zygmunt M. (2003) Eur J Med Chem. 38(6):555-66 Subtelna I, Atamanyuk D, Szymańska E, Kieć-Kononowicz K, Zimenkovsky B, Vasylenko O, Gzella A, Lesyk R. (2010) Bioorg Med Chem. 18(14):5090-102 Li HQ, Yang J, Ma S, Qiao C (2010) Bioorg Med Chem. 20(14): 4194-200

This research was funded by a grant (No.SMT2014P312) from the Research Council of Lithuania

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P-18 THE INFLUENCE OF THE FERMENTED WITH CERTAIN LACTOBACILLI RHAPONTICUM CARTHAMOIDES ON THE QUALITY AND SAFETY OF CURD CHEESE Erika Skabeikyte1, Giedre Jociene1, Elena Bartkiene1, Grazina Juodeikiene2, Daiva Vidmantiene2, Audrius Maruska3, Ona Ragazinskiene4 1

Lithuanian University of Health Sciences, Tilzes g. 18, 47181 Kaunas, Lithuania Kaunas University of Technology, Radvilenu pl. 19, 50254 Kaunas, Lithuania 3 Vytautas Magnus University, Vileikos st. 8-, LT-44404 Kaunas, Lithuania 4 Kaunas Botanical Garden, Vytautas Magnus University, Z. E. Zilibero st. 6, Kaunas LT-46324 Kaunas, Lithuania 2

The aim of this study was to investigate the effects of the fermented with different lactic acid bacteria (LAB) Rhaponticum carthamoides (Rc) on the quality and safety parameters of the curd cheese. For the herbs solid state (SSF) and submerged (SMF) fermentation with Pediococcus acidilactici KTU05-7, Pediococcus pentosaceus KTU05-8 and Lactobacillus sakei KTU05-6 was used. Curd cheese samples were made with SSF and SMF Rc additives. The mass fraction of fermented Rc in the curd cheese was 7.0 %. Control samples were made without Rc additives and with not fermented Rc additives. The concentrations of L-(+) and D-(-) lactic acid in curd cheese samples were determined by an enzyme test kit (R – biopharm AG – Roche, Damstadt, Germany) as reported elsewhere (Yun, Wee, & Ryu 2003). Biogenic amines (BA) analysis was carried out according to the procedures developed by Ben Gigirey et al. (1999). Sensory analysis was carried out with FaceReader software. It was found that the concentration of D-(-) lactic acid isomers in curd cheese with fermented Rc additives range from 0.0002±0.000004 g/100g to 1.78±0.04 g/100g (in samples with SMF with P. pentosaceus Rc and in samples with SSF with P. acidilactici Rc, respectively). The concentration of BA‘s in all analysed samples were far below levels causing a health risk and was ranging from 0.34 to 171.27 mg kg-1(in control samples and in samples with SMF with L. sakei Rc, respectively). Also, fermented herbs hade a positive impact on the sensory properties of the curd cheese. Most acceptable curd cheese samples were found with SSF with P. pentosaceus Rc additive. We conclude that SSF and SMF with in experiment used LAB Rc additives could be used for the curd cheese quality and safety parameters improving, and this is a natural and safe way to increase the curd cheese quality. Keywords: curd cheese, Rhaponticum carthamoides, fermentation, lactobacilli. References: 1. Ben-Gigirey B, De Sousa JMVB, Villa TG., Barros-Velazquez J. 1999. Histamine and cadaverine production by bacteria isolated from fresh and frozen albacore (Thunnus alalunga). Journal of Food Protection. 62: 933–939. 2. Yun, J.S., Wee, Y.J., and Ryu, H.W. 2003. Production of optically pure L-(+)-lactic acid from various carbohydrates by batch fermentation of Enterococcus faecalis RKY1. Enzyme Microbiology Technology. 33. 416-423.

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P-19 EFFECT OF GIBBERELLIC ACID ON LIPID PEROXIDATION IN CHICKPEA (Cicer arietinum L.) SUBJECTED TO SALT AND BORON STRESSES Cekic Fazilet Ozlem Soil salinity is a major environmental stress that affects crop productivity all over the world. Irrigation with saline groundwater containing boron (B) can also affect plant productivity. However, information about the combined effects of salinity and B is very limited. Gibberellins (GAs) are a group of naturally occurring tetracyclic diterpenoids that regulate growth and developmental processes such as seed germination, vegetative growth, flowering induction. Previous studies have shown that gibberellic acid (GA) plays a role in the growth responses of plants during salt and boron stresses. In the present study, the effect of GA was investigated on lipid peroxidation under salt (NaCl), boron (H3BO3) and salt+boron multiple stress in Cicer arietinum L. GA treatment showed an alleviative effect against damage of boron and boron+salt stress on lipid peroxidation. Our results showed that GA can alleviate the imbalances caused by boron and salt+boron toxicity.

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P-20 SOME MULTILOCUS DNA MARKERS APPLICATION FOR ANALYSES OF NATIVE IN LITHUANIA POPULATIONS OF IMPATIENS NOLI-TANGERE L. Goda Sirutaviciute*, Lina Zybartaite, Ramunas Vilcinskas, Algimantas Paulauskas, Eugenija Kupcinskiene Vytautas Magnus University, Department of Biology, Vileikos 8, LT-44404 Kaunas, Lithuania *Corresponding author, e-mail: [email protected]

Balsaminaceae family comprises more than 1000 species. Among representatives of this family three species are present in Lithuania, two of them, Impatiens glandulifera and Impatiens parviflora are aggressive alien species of this country and only Impatiens noli-tangere is native. Valuable information might be obtained comparing genetic variability of alien species and native ones. In Lithuania, molecular studies employing RAPD markers have been carried out for all three Impatiens species [1, 2]. Concerning inter simple sequence repeats (ISSR) markers data is available only for invasive species, while information for Impatiens noli-tangere remains unknown. So the objective of this study was to evaluate the genetic variability at ISSR loci of populations of I. nolitangere differing in geography. Plants were collected from 12 locations. For I. noli-tangere studies four 12-mer primers (ISSR2, ISSR3, ISSR5 and ISSR6) were used as it is described Provan et al. [3]. The work was sponsored by Lithuania Research Council; project No LEK-07/2012. Among populations of I. noli-tangere genetic parameters ranged in the following intervals: 20–50 % of polymorphic DNA bands, 0.06–0.13 for Nei’s gene diversity, 0.1–0.2 for Shannon’s information index. Pairwise genetic distances between populations ranged in the interval 0.03–0.15. Genetic distance-based cluster analyses for 175 individuals indicated 5 major groups of populations, among which there was no clear geographical pattern. On the bases of ISSR markers prepared UPGMA dendrogram of individuals and dendrogram of populations did not show tight relation to population geography. The same was true when principal component analyses were applied for genetic data. Key words: Balsaminaceae, touch-me-not, native species, genetic markers, species diversity [1] Kupcinskiene E, Zybartaite L, Janulioniene R, Zukauskiene J, Paulauskas A (2013) Molecular diversity of small balsam populations in relation to site characteristics. Central European Journal of Biology 8:1048–1061 [ 2 ] Zybartaite L, Zukauskiene J, Jodinskiene M, Janssens SB, Paulauskas A, Kupcinskiene E (2011) RAPD analysis of genetic diversity among Lithuanian populations of Impatiens glandulifera Royle. Zemdirbyste=Agriculture 98:391– 398 [3] Provan J, Love HM, Maggs CA (2007) Development of microsatellites for the invasive riparian plant Impatiens glandulifera (Himalayan balsam) using intersimple sequence repeat cloning. Molecular Ecology Notes 7:451-453

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P-21 THE IMPACT OF AUXIN AND AUXIN INHIBITORS ON BARLEY MOMEOTIC MUTANTS AND THEIR HYBRIDS G. Kaselytė1, V. Okockytė1, R.Šiukšta1,2, V. Vaitkūnienė1,2 1

Department of Botany and Genetics, Vilnius University, M. K. Čiurlionio 21/27, LT-03101 Vilnius,

Lithuania. E-mail: [email protected] 2

Department of Plant Collections, Vilnius University Botanical Garden, Kairėnų 43, LT-10239

Vilnius, Lithuania. E-mail: [email protected] Barley ranks fourth among the cereals both for area and production, therefore investigations of cereal flower structure modification are essential for biotechnological management of crosspollination. It is known, that establishment of auxin gradients is critical to the development of flowers/inflorescences, moreover, exogenous treatment with synthetic auxin 2,4dichlorophenoxyacetic acid (2,4-D) induces variation in flower/inflorescence structure in cereals, thus present research is focused onthe effect of 2,4-D on various Hooded type (Hd) and tweaky spike 2 (tw2) barley homeotic mutants and their hybrids. tw type mutations cause an unfixed transformation of lodicules into sexual organs,accompanied by flower organ number alterations in basal flowers, while Hd type mutations, caused by a duplication in intron IV of BKn3 gene,leads to the development of ectopic flowers of inverted polarity instead of awn or on it. Treatmentwith 2,4-D caused the significant destabilization of basic flower structure in hybrids and mutant tw2, whilepartial structural normalization induced by exogenous 2,4-D was determined in the ectopic flowers of hybrids and Hd type mutants. Sequence analysis of two regulatory regions in BKn3 gene showed that all tested Hd type mutants and their hybrids have the same IIIc (K) allele, indicating that great phenotypic differences in various Hd mutants and their hybrids are not related to the sequence variation in regulatory regions in BKn3 gene. Investigation of the dependenceof callus growth onauxin concentration and auxin inhibitors showed that elevated concentration of 2,4-Das well as auxin transport inhibitor HFCA had only insignificant effect on callus growth, while antiauxin PCIB significantly reduced callus growth rate in most tested genotypes, suggesting that exploration of exogenous PCIB action in planta is likely to be expedient for the future experiments. Key words: auxin inhibitors, barley, callus, ectopic flowers, homeotic mutants, flower structure, 2,4-D

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P-22 OBSERVATION ON BIOLOGICAL PROPERTIES OF THE CHUB, Lueciscus cephalus AND ENVIRONMENT CONDITIONS IN THE ÇAPARLIPATLAK POND (BALIKESIR), SOUTHERN MARMARA REGION, TURKEY Hatice Torcu Koç , Zeliha Erdoğan , and Kazım Bulgen Department of Biology, Faculty of Science and Arts, University of Balikesir University, Cagıs Campus, 10145, Balikesir, Turkey This investigation was conducted in the Çaparlıpatlak Pond, based on a 1-year study involving 431 specimens. The age composition of this species ranged between I and VI with the dominance of fourth age group in the population. Individuals were composed of 36.60% males and 63.40% females. The male and female ratio was 1:1.71 (M:F). Female individuals attained greater size than males. The largest female captured was 21.5 cm FL while the largest male was 20.2 cm FL at the same age, VI. The length–weight relationship was given by W = 0.0648*L2.44 (where W= weight in g; FL= fork length in mm). Age at length data were inferred by modal analysis of the length– frequency distributions. The parameters of the fitted Von Bertalanffy growth equation were L∞ = 23.66 cm; K =0.30yr−1; to=−0.95yr. Macroscopic examination of the gonads, and analysis of the monthly values of the gonadosomatic index, indicated that reproduction occurs in spring and early summer, with a maximum between April and June, when water temperatures are high. Key words: Leuciscus cephalus, age, growth, condition, spawning

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P-23 COMPOSTS WITH HAIR RAPESEED GERMINATION

ADDITIVES

QUALITY

INFLUENCE

ON

Ieva Augunaite1*, Justa Sirvaityte1, Virgilijus Valeika2 and Ilona Jonuskiene3 1

Department of Polymer Chemistry and Technology, 2Department of Physical and Inorganic Chemistry, 3Department of Organic Chemistry, Kaunas University of Technology, Radvilėnų pl. 19, LT-50524 Kaunas, Lithuania;

Hide/skin come to the tanner as a by-product of the meat industry. The use of conventional leather tanning technology is highly polluting because it produces large amounts of organic and chemical pollutants. Wastes generated by the leather processing industries pose a major challenge to the environment. When the hair saving unhairing process is applied, hair becomes waste, which constitutes about 5-10% of dry raw bovine weight. Such waste could be used as fertilizers and source of nitrogen in compost. It should be mentioned that keratin as the main hair protein is highly resistant to degradation. For experiment were chosen differently obtained hair waste. The composting was carried out three months under controlled conditions. To evaluate compost quality the rapeseed (Brassica napus L.) germination experiment was executed. Acknowledgement The research was carried out according to programme EUREKA project VP1-3.1-ŠMM-06-V-01-003 (SALTLESS LEATHER 1.2.1.19) funded by ESFA.

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P-24 CHANGES IN CRUDE OIL HYDROCARBONS COMPOSITION, DURING DEGRADATION BY ARTHROBACTER SP. M1 AND ACINETOBACTER SP. PR82 IN OPTIMAL CONDITIONS Indrė Gailiūtė 1,2, Gražina Račkauskienė 1, Saulius Grigiškis 1,3 1,2,3

JSC „Biocentras“, V. A. Graičiūno st. 10, LT 02241, Vilnius, Lithuania. E-mail:[email protected] Istitute of Biotechnology, Vilnius University, V. A. Graičiūno st. 8, LT 02241, Vilnius, Lithuania. ,3 Vilnius Gediminas Technical University, Saulėtekio al. 11, LT 10223, Vilnius, Lithuania. 2

One of perspective areas of biotechnology application is clean-up of environmental contaminated by oil hydrocarbons. The above biotechnology is based on ability of same microorganisms to degrade the crude oil and oil products. Among the oil hydrocarbon degrading microorganisms the bacteria of genus Arthrobacter and genus Acinetobacter are especially attractive for biotechnological applications, as they are widespread in environment and are able to survive in extreme conditions [1,2]. The aim of this work was to select optimal conditions (inoculums age, inoculums volume, temperature, pH, concentration) for microbial strains degrading crude oil and research, how SARA fractions (saturated compounds, aromatic compounds, resins, asphaltenes) change in composition after crude oil degradation in optimal conditions. Two strains of Acinetobacter sp. Pr82 and Arthrobacter sp. M1 were obtained from culture collection of JSC “Biocentras” and used for investigations. Research results show the best degradation intensity by Acinetobacter sp. Pr82 and Arthrobacter sp. M1 of saturated hydrocarbons, much lower degradation was of aromatic compounds, resins and asphaltenes. Keywords: Crude oil, Arthrobacter sp., Acinetobacter sp., biodegradation, SARA (saturates, aromatics, resins, asphaltenes) fractions References 1. 2.

Levišauskas D.; Tekorius T,; Čipinytė V.; Grigiškis S. 2004. Experimental optimization of nutrient media for cultivation of Arthrobacter sp. Bacteria. Latvijas Kimijas Zurnals. 1: 75-80. Towner K.; 2006. The genus Acinetobacter. Prokaryotes. 6:746-758.

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ISSN 2335-8653

P-25 THE DYNAMICS OF PHOTOSYNTHETIC PARAMETERS OF PHASEOLUS VULGARIS AND VICIA FABO UNDER STRONG CADMIUM STRESS Irena Januškaitienė Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas,Lithuania

E-mail: [email protected] One of the most toxic metals is cadmium, which inputs include those from commercial fertilizers, sewage sludge and other wastes used as soil amendments and also atmospheric deposition. The aim of this experiment was to study the dynamics of the photosynthetic parameters of bean (Phaseolus vulgaris L.) and broad bean (Vicia fabo L.) under strong cadmium stress effect. Plants were sown in a neutral (pH 6.0–6.5) peat substrate, when 2nd true leaf unfolded, growth substrate was watered with 6mM concentration CdSO4 solution. Gas exchange parameters (photosynthetic rate (Pn), transpiration rate (Tn), intercellular CO2 concentration (Ci) and water use efficiency (WUE)) were measured on every day with portable photosynthesis system LI-6400. Content of photosynthetic pigments were analyzed in acetone extract using a spectrophotometer on the last 5th day of the experiment. On the first day of treatment the Pn of Cd treated Phaseolus vulgaris plants decreased only by 4.3% and statistically insignificant, while Pn of Vicia fabo decreased by 35.9% (p