OIE Reference Laboratory Reports Activities Activities in 2014
This report has been submitted : 2015-02-03 15:14:52
Name of disease (or topic) for which you are a designated OIE Reference Laboratory:
African horse sickness
Address of laboratory:
LABORATORIO CENTRAL DE VETERINARIA (LCV) Ctra M-106, pk 1.4 28110 Algete (Madrid) SPAIN Centro de Vigilancia Sanitaria Veterinaria (VISAVET).Universidad Complutense de Madrid (UCM) Avda. de Puerta de Hierro s/n, 28040. Madrid
Tel.:
+34913478312(LCV)/39
Fax:
+34913473778(LCV)/39
E-mail address:
[email protected]
Website:
www.sanidadanimal.info (UCM)
Name (including Title) of Head of Laboratory (Responsible Official):
Montserrat Agüero García, PhD Directora Técnica del LCV-Sanidad Animal Prof. José Manuel Sánchez-Vizcaíno(
[email protected]) Catedrático Dpto de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid.
Name (including Title and Position) of OIE Reference Expert:
Montserrat Agüero García, PhD Directora Técnica del LCV-Sanidad Animal Prof. José Manuel Sánchez-Vizcaíno Catedrático del Departamento de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid.
Which of the following defines your laboratory? Check all that apply:
Governmental
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain
ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Yes
2
OIE Reference Laboratory Reports Activities, 2014
African horse sickness - Montserrat Agüero Garcia - spain
Diagnostic Test
Indicated in OIE Manual (Yes/No)
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
(LCV) ELISA bloqueo
si
336 Preparación suero trabajo+448 preparación colaborativo 2014+ 167 exportaciones+32 plan vigilancia
0
(UCM) ELISA comercial INGENASA Ac)
si
50
(UCM) Neutralización viral
si
25
(UCM) Penside Test (INGENASA Ac)
no
50
Direct diagnostic tests (LCV) Inoculación en cultivos celulares (VERO)
Nationally si
19 (expansión cepas reciente circulación)
(LCV) RRT-PCR Agüero et al.2008 (VP7)
si
320 preparación colaborativo 2014+590 preparación ensayo intercomparación OIE+ 240 participación ensayo intercomparación OIE
(LCV) RRT-PCR Fernández-Pinero et al.2008
si
240 participación ensayo intercomparación OIE
(LCV) Monaco et al.2010
no
240 participación ensayo intercomparación OIE
(LCV) Quan et al. 2010
si
240 participación ensayo intercomparación OIE
(UCM) RRT-PCR Fernández-Pinero et al.2008
si
200
(UCM) RRT-PCR Agüero et al.2008 (VP7)
si
150
(UCM) Inoculación en cultivos celulares (VERO)
si
40
(UCM) Inoculación en cultivos celulares (BHK-21)
si
10
Internationally
ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain
To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Yes
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OIE Reference Laboratory Reports Activities, 2014
African horse sickness - Montserrat Agüero Garcia - spain
Type of reagent available
(LCV)VPEA de los serotipos 6,7 y 8, virus de la EEV serotipo 3, inactivados, enviados en el ensayo de intercomparación organizado por el EU-RL (LCV)Suero equino enviado en el ensayo de intercomparación organizado por el EU-RL (animales vacunados frente a los serotipos 4 o 9, o animales infectados experimentalmente con el serotipo 9)
(LCV) Suero de Referencia RS13 liofilizado
(LCV)PT OIE Panel 2 (VPEA de todos los serotipos, virus de la EEV serotipo 3, inactivados)
(LCV)VPEA de los serotipos 2 y 5
Related diagnostic test
RT-PCR
ELISA
ELISA
RT-PCR
RT-PCR
Produced/ provide
10 ml/virus inactivado
10 ml/suero
10 ml de suero
5 ml/virus inactivado
1 ml/serotipo
Amount supplied nationally (ml, mg)
0
Amount supplied internationally (ml, mg)
No. of recipient OIE Member Countries
Region of recipients
600μl/muestra (10 muestras por país participante)
19 países miembros de la UE, Suiza, Marruecos (2 laboratorios), Turquía, Túnez, Singapur y Méjico
Africa Americ as Asia and Pacific Europe Middle East
300μl/muestra (10 muestras por país participante)
24 países miembros de la UE, Suiza, Marruecos, Turquía, Túnez, Singapur y México
Africa Americ as Asia and Pacific Europe Middle East
300μl/muestra
24 países miembros de la UE, Suiza, Marruecos, Turquía, Túnez, Singapur y México
Africa Americ as Asia and Pacific Europe Middle East
Sudáfrica, USA, Francia, España, Reino Unido
Africa Americ as Asia and Pacific Europe Middle East
Letonia
Africa Americ as Asia and Pacific Europe Middle East
500μl/muestra (45 muestras por país participante)
2 ml
4. Did your laboratory produce vaccines? No
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain 5. Did your laboratory supply vaccines to OIE Member Countries? No
ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? No
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy
Purpose
How the advice was provided
UNITED ARAB EMIRATES
(LCV)Asesoramiento en métodos de multiplicación del VPEA en células VERO
A distancia (vía correo electrónico)
LATVIA
(LCV)Mejorar las técnicas de detección de ácidos nucléicos (VPEA)mediante RRT-PCR
A distancia (vía correo electrónico)
SPAIN
(UCM)Análisis de riesgo, modelización sanitaria, educación continuada para la red de alerta sanitaria veterinaria (RASVE)
Proyecto
ToR 5: To carry out and/or coordinate scientific and technical studies in
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OIE Reference Laboratory Reports Activities, 2014
African horse sickness - Montserrat Agüero Garcia - spain
collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes
Title of the study
Duration
Purpose of the study
Partners (Institutions)
3 años
Desarrollar nuevas herramientas que mejoren la detección temprana de enfermedades animales
Proyecto Europeo con 12 instituciones asociadas
3 años
Desarrollar nuevas herramientas que mejoren la vigilancia de enfermedades animales.
Proyecto Europeo con 12 instituciones asociada
(UCM)Rapid Field Diagnostics and Screening in Veterinary Medicine (FP7KBBE-2011-5)
(UCM)Providing a new generation of methodologies and tools for cost-effective risk-based animal health surveillance systems for the benefit of livestock producers, decision makers and consumers (RISKSUR). (FP7-KBBE.2012.1.3-01)
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control? Yes 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes
13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 UCM: Rodríguez-Prieto V., Vicente-Rubiano M., Sánchez Matamoros A., Rubio-Guerri C., Melero M., Martínez-López B., M. Martínez-Avilés M., Hoinville L,, Vergne T., Comin A., Schauer B., Dórea F., Pfeiffer D.U. and Sánchez-Vizcaíno J.M. Systematic review of surveillance systems and methods for early detection of exotic, new and re-emerging diseases in animal populations Epidemiology and Infection (2014). Sep 12:1-25 Sánchez Matamoros A., Sánchez-Vizcaíno J.M., Rodríguez-Prieto V., Iglesias E. and Martínez-López B. Identification of suitable areas for African horse sickness virus infections in Spanish equidae populations. Transboundary and Emerging Disease (2014). Dec 5. doi: 10.1111/tbed.12302. Sánchez Matamoros A., Beck C., Kukielka D. Lecollinet S., Blaise-Boisseau S., Garnier A., Rueda P., Zientara S. and Sánchez-Vizcaíno J.M. 2015. Development of a Luminex assay for the serological detection of African Horse Sickness virus in horses: comparison with other diagnostic techniques. Transboundary and Emerging Disease. Aceptado Sánchez Matamoros A., Kukielka D. and Sánchez-Vizcaíno J.M. Cytokine expression in horse exposed to African
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain Horse Sickness Virus and its relation to the clinical form. Research in Veterinary Science (2014). En revisión. Sánchez Matamoros A., Nieto-Pelegrín E., Beck C., Lecollinet S., Rivera-Arroyo B., Blaise-Boisseau S., Garnier A., Zientara S. and Sánchez-Vizcaíno J.M. Development of a DIVA Luminex assay for the serological detection of African horse sickness in horses. 2014. En preparación b) International conferences: 5 LCV: IV International Conference on Bluetongue and related orbiviruses. Auditorium Antonianum, Rome, 5-7 Noviembre, 2014 Presentación oral: Tena, C.; SINGLE AND MULTIPLEX REAL TIME RT-PCR ASSAYS SEROTYPE SPECIFIC TO DETECT AFRICAN HORSE SICKNESS VIRUS UCM: Sánchez-Matamoros A., Beck C., Kukielka D., Lecollinet S., Blaise-Boisseau S., Garnier A., Rueda P., Zientara S. and Sánchez-Vizcaíno J.M. Comunicación oral: New Diagnostic Technique for Serological Detection of African Horse Sickness Virus: Luminex Technology. EPIZONE. 8th Annual Meeting, septiembre 2014. Sastre P., Pérez T., Tapia I., Sánchez-Matamoros A., Sánchez-Vizcaíno J.M., Rueda P., and Sanz A. Poster: New diagnostic tools for African Horse Sickness and Equine Infectious Anemia viruses control. EPIZONE. 8th Annual Meeting, septiembre 2014. Sánchez Matamoros A., and Sánchez-Vizcaíno J.M. Comunicación oral: Development of Luminex assay for the serological detection of African Horse Sickness Virus in horses and DIVA diagnosis. RAPIDIA Meeting, mayo 2014. Vicente-Rubiano M., Rodríguez-Prieto V., Martínez-Avilés M., Sánchez-Matamoros A., Rubio-Guerri C., Melero M., Martínez-López B., Hoinville L,, Vergne T., Comin A., Schauer B., Dórea F., Pfeiffer D.U. and Sánchez-Vizcaíno J.M. Poster: Literature review of surveillance systems and approaches for the early detection of new, exotic and re‐ emerging diseases. Society for Veterinary Epidemiology and Preventive Medicine (SVEPM). 2014 Annual Conference, marzo 2014. c) National conferences: 2 UCM: Sánchez-Matamoros A., Rubio Guerri C. y Nieto-Pelegrín E. Comunicación oral: Nuevas herramientas de diagnóstico en enfermedades animales: Metagenómica, Luminex y Proteómica. Virología en Sanidad Animal, junio 2014. Trobiani Gallegos C., Rodríguez Alepuz D. Tutorizado por Sánchez-Matamoros A. y Sánchez-Vizcaíno J.M. Comunicación oral: Nuevo Diagnóstico DIVA de Peste Equina Africana. IX Jornadas Complutenses, VIII Congreso Nacional de Investigación en Ciencias de la Salud para Alumnos Pregraduados y XIII Congreso de Ciencias Veterinarias y Biomédicas, abril 2014. d) Other: (Provide website address or link to appropriate information) 5 LCV: Reunión anual de laboratorios nacionales de referencia de Estados Miembros de la UE para Peste Equina Africana. Auditorium Antonianum, 4 Noviembre, Roma, 2014. - Tena, C. Results of the Proficiency Test 2014. - Durán, M. Assessment of AHS EU-RL/NRLs network during 2009-2013 - Durán, M. Performance Indicators / Good, better and best practices. - Agüero, M.Review of diagnostic methods recommended for movement of equidae (Directive 2009/156/CE). Assessment of published RRT-PCR methods for AHSV detection UCM: Internet: Actualización epidemiológica de la Peste Equina Africana en la sección de "Enfermedades emergentes" de la página web (www.sanidadanimal.info).
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OIE Reference Laboratory Reports Activities, 2014
African horse sickness - Montserrat Agüero Garcia - spain
ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? Yes a) Technical visits: 0 b) Seminars: 0 c) Hands-on training courses: 1 d) Internships (>1 month): 0
Type of technical training provided (a, b, c or d)
Country of origin of the expert(s) provided with training
No. participants from the corresponding country
c (brindado por la UCM)
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard? Yes
Quality management system adopted
Certificate scan (PDF, JPG, PNG format)
(LCV) UNE EN ISO 17025
Anexo Técnico ENAC Rev.7 + LEBA_LCV (5.12.2014).pdf
(LCV) UNE EN ISO 14001
CertificadoGA-2002-0327_ES_2015-01-14.jpg
(UCM) ISO 17025
Certificado ISO17025.pdf
16. Is your laboratory accredited by an international accreditation body? Yes
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain Test for which your laboratory is accredited
Accreditation body
(LCV) Detección de anticuerpos frente al VPEA mediante ELISA en sueros de équidos
ENAC
(LCV) Detección de orbivirus mediante cultivo celular (BTV, AHSV, EHDV) en muestras de sangre completa no coagulada, bazo, pulmón y nódulos linfáticos
ENAC
(LCV) Detección-identificación del VPEA medianteRT-PCR a tiempo real en muestras de vísceras de animales, sangre y cultivos celulares
ENAC
(LCV) Serotipificación de virus del género Orbivirus por neutralización (BTV, AHSV, EHDV) en suero de rumiantes y équidos
ENAC
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? Yes (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a)
ToR 9: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE? No
19. Did your laboratory participate in scientific meetings on behalf of the OIE? Yes
Title of event
Date (mm/yy)
Location
Role (speaker, presenting poster, short communications)
Title of the work presented
Third Global Conference of OIE Reference Centres CHALLENGES AND EXPECTATIONS FOR THE FUTURE
Octubre/2014
Seul
Asistente
-
ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?
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OIE Reference Laboratory Reports Activities, 2014
African horse sickness - Montserrat Agüero Garcia - spain Yes 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes
Purpose of the proficiency tests: 1
(LCV)Evaluación de técnicas de diagnóstico molecular mediante RT-PCR para la detección del VPEA
(UCM)Evaluación de técnicas de diagnóstico molecular mediante RT-PCR para la detección del VPEA
1
Role of your Reference Laboratory (organiser/ participant)
No. participants
Participating OIE Ref. Labs/ organising OIE Ref. Lab.
7
-LAboratorio Central de Veterinaria; Algete, España -Universidad Complutense; Madrid, España. -The Pirbrigth Institute; Pirbrigth, Reino Unido. -Ondersteport Veterinary Institute; Ondersteport, Sudáfrica.
7
-LAboratorio Central de Veterinaria; Algete, España -Universidad Complutense; Madrid, España. -The Pirbrigth Institute; Pirbrigth, Reino Unido. -Ondersteport Veterinary Institute; Ondersteport, Sudáfrica.
organizador y participante
participante
validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Yes
Title of the project or contract
Scope
Name(s) of relevant OIE Reference Laboratories
(LCV)Envío de muestras de animales infectados con el VPEA
Preparación de panel muestras de animales para el esnsayo de intercomparación de la OIE
Laboratorio de Ref de la OIE para la PEA (Ondersteport Veterinary Institute)de Ondersteport, Sudáfrica
(LCV)Calendario ensayo de intercomparación de la OIE
Organización de un programa para la realización del ensayo de intercomparación de la OIE
Laboratorio de Ref de la OIE para la PEA (The Pirbrigth Institute) de Pirbright, UK
(LCV)Evaluación de técnicas de diagnóstico molecular mediante RT-PCR para la detección del VPEA
Detección de VPEA en muestras de sangre o téjido
Lab Ref de la OIE para la PEA de Ondersteport (Sudáfica), Pirbrigth (UK)y Universidad Complutense (MAdrid)
(UCM)Rapid Field Diagnostics and Screening in Veterinary Medicine (FP7-KBBE-2011-5)
Desarrollar nuevas herramientas que mejoren la detección temprana de enfermedades animales.
FRIEDRICH LOEFFLER INSTITUT, THE INSTITUTE FOR ANIMAL HEALTH
OIE Reference Laboratory Reports Activities, 2014
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African horse sickness - Montserrat Agüero Garcia - spain
ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Region(s) of participating OIE Member Countries
No. participating laboratories
Purpose for inter-laboratory test comparisons1
(LCV)Organización de un ensayo de intercomparación de aptitud para la evaluación de métodos de diagnóstico (detección de anticuerpos y genoma viral)
32
Africa Americas Asia and Pacific Europe Middle East
(LCV+UCM)Ensayo de intercomparación de la OIE para la Evaluación de técnicas de diagnóstico molecular mediante RT-PCR para la detección del VPEA
7 (4 lab. ref. OIE detallados en punto 21+ ANSES, Francia; USDA, USA; Deltamune, Sudáfrica)
Africa Americas Asia and Pacific Europe Middle East
ToR 12: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE? Yes
Kind of consultancy
Location
Subject (facultative)
(LCV)Grupo ad hoc para "Evaluation of African Horse Sickness Status of Member Countries"
París enero 2014
Evaluation of African Horse Sickness (AHS) disease of member countries
25. Additional comments regarding your report: En cada apartado las actividades realizadas por cada laboratorio se indican mediante LCV si la ha realizado el Laboratorio Central de Veterinaria o UCM si ha sido realizado por la Universidad Complutense de MAdrid.
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OIE Reference Laboratory Reports Activities, 2014
