OIE Reference Laboratory Reports Activities Activities in 2015
This report has been submitted : 2016-01-15 11:25:50
Name of disease (or topic) for which you are a designated OIE Reference Laboratory:
African horse sickness
Address of laboratory:
LABORATORIO CENTRAL DE VETERINARIA (LCV) Ctra M-106, pk 1.4 28110 Algete (Madrid) SPAIN Centro de Vigilancia Sanitaria Veterinaria (VISAVET).Universidad Complutense de Madrid (UCM) Avda. de Puerta de Hierro s/n, 28040. Madrid Tel:0034 913944082
Tel.:
+34 913 47 83 12 (LC
Fax:
+34 913 47 37 78 (LC
E-mail address:
[email protected]
Website:
www.sanidadanimal.info (UCM)
Name (including Title) of Head of Laboratory (Responsible Official):
Montserrat Agüero García, PhD Directora Técnica del LCV-Sanidad Animal Prof. José Manuel Sánchez-Vizcaíno(
[email protected]) Catedrático Dpto de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid.
Name (including Title and Position) of OIE Reference Expert:
Montserrat Agüero García, PhD Directora Técnica del LCV-Sanidad Animal Prof. José Manuel Sánchez-Vizcaíno Catedrático del Departamento de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid
Which of the following defines your laboratory? Check all that apply:
Governmental
OIE Reference Laboratory Reports Activities, 2015
1
African horse sickness - Montserrat Agüero Garcia - spain
ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Yes
2
OIE Reference Laboratory Reports Activities, 2015
African horse sickness - Montserrat Agüero Garcia - spain
Diagnostic Test
Indicated in OIE Manual (Yes/No)
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
(LCV ELISA bloqueo)
SI
568 (Sueros diagnóstico 344+28 plan vigilancia+Colaborativo=14xduplicado x 2 técnicos, 14 x duplicado x 2 lote, 14 x duplicado x 3 paneles)
82 (Dubai=41 x duplicado)
(LCV)Seroneutralización
SI
(VISAVET) ELISA comercial (INGENASA Ac)
SI
52
(VISAVET) Neutralización viral
SI
20
(VISAVET) Penside test (INGENASA Ac)
SI
60
738 (Dubai=41 x duplicado x 9 serotipos)
Direct diagnostic tests
Nationally
Internationally
SI
32 (Expansión Cepas de trabajo Serotipos 7 y 8 (células VERO y BHK))
124 (Dubai= Aislamiento tejidos (sangre, bazo, pulmón, hígado, corazón) 1PKC-2PBHK-3PBHK)
(LCV)RRT- PCR Agüero et al.2008 (VP7)
SI
430 (Colaborativo: 10 muestrasxduplicadox8 paneles+ 27 países x 10 muestras)
372 (Dubai= 1PKC-2PBHK-3PBHK=31 tejidos x cuadruplicado x 3 pases)
(LCV)RRT- PCR Multiplex (1,6,8)
NO
124 (Dubai= 1PKC-2PBHK-3PBHK=31 x cuadruplicado)
(LCV)RRT- PCR Multiplex (2,4,9)
NO
124 (Dubai= 1PKC-2PBHK-3PBHK=31 x cuadruplicado)
(LCV)RRT- PCR Multiplex (3,5,7)
NO
124 (Dubai= 1PKC-2PBHK-3PBHK=31 x cuadruplicado)
(VISAVET) RT-PCR (sonda Taqman MGB) Fernández-Pinero et al. 2009
SI
250
(VISAVET)RT-PCR (sonda Taqman MGB) Agüero et al. 2008
SI
200
(LCV) Inoculación en cultivos celulares
OIE Reference Laboratory Reports Activities, 2015
3
African horse sickness - Montserrat Agüero Garcia - spain (VISAVET) Aislamiento y multiplicación en células BHK-21
SI
5
(VISAVET) Aislamiento y multiplicación en células Vero
SI
30
ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Yes
Type of reagent available
(LCV)VPEA de los serotipos 4,6,7 virus de la EEV serotipo 3, inactivados, enviados en el ensayo de intercomparación organizado por el EURL (LCV)Suero equino enviado en el ensayo de intercomparación organizado por el EURL (animales vacunados frente a los serotipos 4 o 9, o animales infectados experimentalmente con el serotipo 9)
Related diagnostic test
RT-PCR
ELISA
Produced/ provide
10 ml/virus inactivado
10 ml/suero
Amount supplied nationally (ml, mg)
0
0
Amount supplied internationally (ml, mg)
No. of recipient OIE Member Countries
Region of recipients
600μl/muestra (10 muestras por país participante)
19 países miembros de la UE, Suiza, Marruecos (2 laboratorios), Turquía, Túnez, Singapur,Méjico y Dubai
Africa Americ as Asia and Pacific Europe Middle East
300μl/muestra (10 muestras por país participante)
24 países miembros de la UE, Suiza,AHVLA Weybridge, Marruecos (2 laboratorios), Turquía, Túnez, Singapur,Méjico y Dubai
Africa Americ as Asia and Pacific Europe Middle East
4. Did your laboratory produce vaccines? No
4
OIE Reference Laboratory Reports Activities, 2015
African horse sickness - Montserrat Agüero Garcia - spain 5. Did your laboratory supply vaccines to OIE Member Countries? No
ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? Yes 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No Name of the new test or diagnostic method or vaccine developed
Description and References (Publication, website, etc.)
(LCV) Agüero et al. real-Time fluorogenic reverse transcription polymerase chain reaction assay for detection of African Horse Sickness virus
J.Vet. Diagnostic Invest (2008) 20(3):325-8 Dossier presented to OIE Biological Standard Commission for "Validation of the real-time reverse transcription PCR (RRT-PCR) method for detection of African Horse Sickness Virus (AHSV) genome, described by Agüero et al.2008" on 13th August 2015. Accepted by OIE BSC during its meeting Paris 1-3 September 2015. Available under request at
[email protected]
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes
Name of OIE Member Country seeking assistance
Date (month)
No. samples received for provision of diagnostic support
No. samples received for provision of confirmatory diagnoses
UNITED ARAB EMIRATES
Enero 2015
0
4
UNITED ARAB EMIRATES
Julio 2015
4
30
UNITED ARAB EMIRATES
Agosto 2015
3
7
UNITED ARAB EMIRATES
Octubre 2015
14
10
UNITED ARAB EMIRATES
Noviembre 2015
6
2
OIE Reference Laboratory Reports Activities, 2015
5
African horse sickness - Montserrat Agüero Garcia - spain 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy
Purpose
How the advice was provided
UNITED ARAB EMIRATES
(LCV) Apoyo y asesoramiento en diagnóstico de VPEA en muestras de equinos procedentes de Kenia. Apoyo al diagnóstico mediante técnicas de ELISA, VN de VPEA, Aislamiento del VPEA en cultivos celulares VERO, KC y BHK. Realización RT-PCR, PCR convencional de serotipo.
A distancia (vía correo electrónico)
UNITED ARAB EMIRATES
(LCV)Mejorar las técnicas de detección de ácidos nucleicos (VPEA) mediante RRT-PCR
A distancia (vía correo electrónico)
SINGAPORE
(LCV)Mejorar las técnicas de detección de ácidos nucleicos (VPEA) mediante RRT-PCR
A distancia (vía correo electrónico)
ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes
6
OIE Reference Laboratory Reports Activities, 2015
African horse sickness - Montserrat Agüero Garcia - spain Title of the study
(LCV) To perform a comparative study of a variety of suitable RTPCR methods for AHSV genome detection
(LCV and VISAVET) Re-validate AHSV Blocking VP7 ELISA, validate LFD and possibly AHSV NS3 ELISA
(VISAVET) Rapid Field Diagnostics and Screening in Veterinary Medicine (FP7KBBE-2011-5)
(VISAVET) Providing a new generation of methodologies and tools for cost-effective risk-based animal health surveillance systems for the benefit of livestock producers, decision makers and consumers (RISKSUR). (FP7KBBE.2012.1.3-01)
Duration
Purpose of the study
Partners (Institutions)
OIE request to the AHS OIE experts for an opinion on the adoption of an AHS RT-PCR as a prescribed test for international trade
-Dr. Alison Lubisi, ARC-OVI, OIE ref. laboratory (South Africa) Dr.Javier Castillo, Pirbright Institute, OIE ref laboratory (Uk) Prof. J. Manuel Sánchez-Vizcaíno, UCM, OIE ref.laboratory (Spain) Dr. Stephan Zientara, ANSES/INRA/ENVA (France) Dr.Montserrat Agüero, LCV, OIE Ref. laboratory (Spain). -Dr. Eileen Ostlund, USDA (USA). -Dr. Christian Potgieter, Deltamune (South-Africa)
2015-2016
Project from the OIE International Call for tender 2015
The consortion detailed in the above collaborationstudy , and in addition: Dr. Paloma Rueda, INGENASA, Spain. Dr. Federica Monaco, IZ dell´Abruzzo e Molise, Italy
3 AÑOS
Desarrollar nuevas herramientas que mejoren la detección temprana de enfermedades animales.
Proyecto Europeo con 12 instituciones asociadasAlemania, Bélgica, España, Francia, Hungría, Reino Unido, Suecia, Suiza.
3 AÑOS
Desarrollar nuevas herramientas que mejoren la vigilancia de enfermedades animales.
Proyecto Europeo con 12 instituciones asociadaAlemania, Bélgica, Italia, Holanda, Noruega, España, Francia, Reino Unido, Suecia, Suiza.
2014-2015
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control? Yes 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes
13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 (VISAVET)
OIE Reference Laboratory Reports Activities, 2015
7
African horse sickness - Montserrat Agüero Garcia - spain -Sánchez-Matamoros A, Beck C, Kukielka D, Lecollinet S, Blaise-Boisseau S, Garnier A, Rueda P, Zientara S, Sánchez-Vizcaíno JM. "Development of a Microsphere-based Immunoassay for Serological Detection of African Horse Sickness Virus and Comparison with Other Diagnostic Techniques". Transbound Emerg Dis. In press (Online 2/2015) -Sánchez-Matamoros, A., Sánchez-Vizcaíno, JM., Rodríguez-Prieto, V., Iglesias, E., Martínez-López., B. “Identification of Suitable Areas for African Horse Sickness Virus Infections Spanish Equine Populations”. Transbound Emerg Dis. In press (Online 7/2014) b) International conferences: 6 (LCV) -Regional Seminar for OIE National Focal Points for Veterinary laboratories. (Cluj Napoca, Rumania, 27-29 October 2015). Agüero, M. OIE Reference Laboratory Network: Application of terms of reference and experience. - AHSV Ring Trial Workshop (Pirbright, 24th June 2015): Agüero, M. AHSV RT-PCR International Ring Trial. EU Bluetongue Virus and African Horse Sickness Virus Reference Laboratory EURL/NRLs Workshop 2015 (25th November 2015, Sunningdale, UK) -Durán, M. "Results of Proficiency Test 2015" -Durán, M. "The EU-RL Work Program 2016-2017" -Agüero, M. "Review of diagnostic methods recommended for movement of equidae Final Outcome (Directive 2009/156/CE)" -Agüero, M. "Collaborative trial for the assessment of RRT-PCR methods for AHSV detection in the framework of international trade". (VISAVET) -Sánchez Matamoros A., and Sánchez-Vizcaíno J.M. Comunicación oral: Development of Luminex assay for the serological detection of African Horse Sickness Virus in horses and DIVA diagnosis. International Ring Trial on Molecular Diagnostic Methods for African horse sickness 8th Annual Meeting. The Pirbright Institute, junio 2015. -Sánchez-Matamoros A. and Sánchez-Vizcaíno J.M. Discussion and Conclusions of the ring trial 2014 Annual Conference. International Ring Trial on Molecular Diagnostic Methods for African horse sickness. The Pirbright Institute, junio 2015. c) National conferences: 0
d) Other: (Provide website address or link to appropriate information) 2 (VISAVET) -Internet: Actualización epidemiológica de la Peste Equina Africana en la sección de "Enfermedades emergentes" de la página web (www.sanidadanimal.info). -Tesis doctoral: Sánchez-Matamoros A., Iglesias E, Kukielka D and Sanchez-Vizcaino JM. Evolución Epidemiológica y Control de la Peste Equina Africana. Universidad Complutense de Madrid, febrero 2015.
ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? No
8
OIE Reference Laboratory Reports Activities, 2015
African horse sickness - Montserrat Agüero Garcia - spain
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard? Yes
Quality management system adopted
Certificate scan (PDF, JPG, PNG format)
(LCV) UNE EN ISO 17025
Anexo Técnico Acreditación Rev.07 LCV+LEBA Rev.12.pdf
(LCV) UNE EN ISO 14001
CertificadoGA-2002-0327_ES_2015-01-14.jpg
(VISAVET) ISO 17025//817/LE1410 16. Is your laboratory accredited by an international accreditation body? Yes Test for which your laboratory is accredited
Accreditation body
(LCV) Detección de anticuerpos frente al VPEA mediante ELISA en sueros de équidos
ENAC
(LCV) Detección de orbivirus mediante cultivo celular (BTV, AHSV, EHDV) en muestras de sangre completa no coagulada, bazo, pulmón y nódulos linfáticos
ENAC
(LCV) Detección-identificación del VPEA medianteRT-PCR a tiempo real en muestras de vísceras de animales, sangre y cultivos celulares
ENAC
(LCV) Serotipificación de virus del género Orbivirus por neutralización (BTV, AHSV, EHDV) en suero de rumiantes y équidos
ENAC
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? Yes (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a)
ToR 9: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE? No
19. Did your laboratory participate in scientific meetings on behalf of the OIE? Yes
OIE Reference Laboratory Reports Activities, 2015
9
African horse sickness - Montserrat Agüero Garcia - spain
Date (mm/yy)
Location
Role (speaker, presenting poster, short communications)
Title of the work presented
(LCV)Regional Seminar for OIE National Focal Points for Veterinary Laboratories
October 2015
Cluj Napoca, Rumania
Speaker, Montserrat Agüero
application of terms of reference and experience
(VISAVET)AHS-OIE Proyect Initial Meeting
Octubre/2015
Plowright Building
Asistencia
Title of event
ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Yes 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes
Purpose of the proficiency tests: 1
(LCV)Evaluación de técnicas de diagnóstico molecular mediante RTPCR para la detección del VPEA
(VISAVET)Ensayo interlaboratorial
1
Role of your Reference Laboratory (organiser/ participant)
Organizador y participante
Participantes
No. participants
Participating OIE Ref. Labs/ organising OIE Ref. Lab.
7
-LAboratorio Central de Veterinaria; Algete, España -Universidad Complutense; Madrid, España. -The Pirbrigth Institute; Pirbrigth, Reino Unido. -Ondersteport Veterinary Institute; Ondersteport, Sudáfrica
7
-LAboratorio Central de Veterinaria; Algete, España -Universidad Complutense; Madrid, España. -The Pirbrigth Institute; Pirbrigth, Reino Unido. -Ondersteport Veterinary Institute; Ondersteport, Sudáfrica
validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Yes
10
OIE Reference Laboratory Reports Activities, 2015
African horse sickness - Montserrat Agüero Garcia - spain Title of the project or contract
Scope
Name(s) of relevant OIE Reference Laboratories
(LCV) Evaluación de resultados del AHSV RT-PCR International Ring Trial
Evaluación de Técnicas de diagnóstico molecular de AHSV
-LAboratorio Central de Veterinaria; Algete, España -Universidad Complutense; Madrid, España. -The Pirbrigth Institute; Pirbrigth, Reino Unido. -Ondersteport Veterinary Institute; Ondersteport, Sudáfrica
(LCV) Elaboración y envío de Dossier a la Comisión de estandares biológicos de la OIE
Validación del método de RRT-PCR descrito por Agüero et al 2008 para la detección del genoma del AHSV
- EU-RL AHS LCV (Algete) -Pirbright Institute, UK
"Validation study of a serological diagnostic assay for African Horse Sickness"
-Dr.Alison Lubisi (OVI, South Africa). -Dr. Montserrat Agüero,(LCV Algete Madrid). -Dr. Shirley Smith (Deltamune, South Africa).Prof.José Manuel Sánchez-Vizcaino (UCM, Madrid).-Prof. Stephan Zientara, (ANSES Alfort, Paris, France).-Dr. Eileen Ostlund (USDA, Ames, Iowa, USA). -Dr. Paloma Rueda INGENASA, Madrid, Spain- Dr. Federica Monaco, IZ dell´Abruzzo e Molise, Italy. -Dr. Carrie Batten, The Pribright Institute, UK.-Dr. Javier CastilloOlivares, The Pirbright Institute.
(LCV and VISAVET) AD/SR/2015/1855 "Validation study of a serological diagnostic assay for African Horse Sickness"
ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Purpose for inter-laboratory test comparisons1
(LCV)Organización de un ensayo de intercomparación de aptitud para la evaluación de métodos de diagnóstico (detección de anticuerpos y genoma viral)
OIE Reference Laboratory Reports Activities, 2015
No. participating laboratories
33
Region(s) of participating OIE Member Countries Africa Americas Asia and Pacific Europe Middle East
11
African horse sickness - Montserrat Agüero Garcia - spain
ToR 12: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE? Yes
Kind of consultancy
Location
Subject (facultative)
(LCV)Grupo ad hoc para "Evaluation of African Horse Sickness Status of Member Countries"
París, Enero 2015
Evaluation of African Horse Sickness (AHS) disease of member countries
25. Additional comments regarding your report:
12
OIE Reference Laboratory Reports Activities, 2015
