OIE Reference Laboratory Reports Activities Activities in 2013
This report has been submitted : 2014-01-30 18:16:18
Name of disease (or topic) for which you are a designated OIE Reference Laboratory:
Salmonellosis
Address of laboratory:
OIE Reference Laboratory for Salmonellosis Laboratory for Foodborne Zoonoses Public Health Agency of Canada 110 Stone Road West Guelph, Ontario N1G 3W4 CANADA
Tel.:
+1-519 822.33.00
Fax:
+1-519 822.22.80
e-mail address:
[email protected]
website:
www.phac-aspc.gc.ca
Name (including Title) of Head of Laboratory (Responsible Official):
Dr. Roger Johnson, Research Scientist, Director of Reference Laboratories
Name (including Title and Position) of OIE Reference Expert:
Dr. Cornelis Poppe, Research Scientist
Which of the following defines your laboratory? Check all that apply:
Governmental
OIE Reference Laboratory Reports Activities, 2013
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Salmonellosis - Cornelis Poppe - canada
ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Yes
Diagnostic Test
Indicated in OIE Manual (Yes/No)
Total number of test performed last year
Indirect diagnostic tests 0
NA
Direct diagnostic tests
Nationally
Internationally
0
0
Nationally
Internationally
Serotyping
Yes
5579
198
Phagetyping
Yes
1906
0
ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? No 4. Did your laboratory produce vaccines? No
5. Did your laboratory supply vaccines to OIE Member Countries? No
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OIE Reference Laboratory Reports Activities, 2013
Salmonellosis - Cornelis Poppe - canada
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes
Name of OIE Member Country seeking assistance
Date (month)
No. samples received for provision of diagnostic support
No. samples received for provision of confirmatory diagnoses
TRINIDAD AND TOBAGO
May 2013
183
183
ETHIOPIA
August 2013
119
119
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy
Purpose
How the advice was provided
ETHIOPIA
To give advice regarding the isolation, serotyping and determination of antimicrobial resistance of Salmonella isolates
Remote
TURKEY
To give advice regarding the isolation, serotyping and determination of antimicrobial resistance of animal isolates of Salmonella
Remote
BRAZIL
Recommmended the use of standardized methods such as ISO-method 6579:2002 for the isolation and detection of Salmonella from primary animal production as described in the OIE Terrestrial Manual 2012, pages 1268-1286
Remote
TURKEY
Recommended the use of standardized methods for the isolation of Salmonella from faeces
Remote
INDIA
Gave advice regarding the determination of therapeutic efficacy when employing vaccination and the use of bacteriophages to control Salmonella in poultry
Remote
OIE Reference Laboratory Reports Activities, 2013
3
Salmonellosis - Cornelis Poppe - canada
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes
Title of the study
Duration
Purpose of the study
Partners (Institutions)
Development of a Salmonella genoserotyping array (SGSA)
6 years
To rapidly generate antigenic formula consistent with the WhiteKauffmann-Le Minor scheme
Vet. Lab. Agency, Weybridge, UK
6 years
To rapidly generate antigenic formula consistent with the WhiteKauffmann-Le Minor scheme
Austrian Institute of Technology, Austrian Agency for Health and Food Safety, Vienna, Austria
Development of a Salmonella genoserotyping array (SGSA)
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control? Yes 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes
13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 17 1. Frey SK, Topp E, Edge T, Fall C, Gannon VPJ, Jokinen CC, Marti R, Neumann N, Ruecker N, Wilkes G, Lapen DR. Using SWAT, Bacteroidales microbial source tracking markers and fecal indicator bacteria to predict waterborne pathogen occurrence in an agricultural watershed. Water Res 2013; 47(16):6326-6337. 2. David JM, Sanders P, Bemrah N, Granier SA, Denis M, Weill F-X, Guillemot D, Watier L. Attribution of the French human salmonellosis cases to the main food-sources according to the type of surveillance data. Prev Vet Med 2013; 110(1):12-27. 3. Zhao E-Y, Bao H-X, Tang L, Zou QH, Liu W-Q, Zhu D-L, Chin J, Dong Y-Y, Li Y-G,Cao F-L, Poppe C, Sanderson KE, Johnston RN, Zhou D, Liu G-R, Liu S-L. Genomic comparison of Salmonella typhimurium DT104 with non-DT104 strains. Mol Genet Genomics 2013;288(11):549-557. 4. Kabore H, Lebel A, Levallois P, Michel P, Payment P, Dery P, Lebel G. Multilevel analysis of childhood nonviral gastroenteritis associated with environmental risk factors in Quebec, 1999-2006. J Environm Health 2013; 76(3):34-45.
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OIE Reference Laboratory Reports Activities, 2013
Salmonellosis - Cornelis Poppe - canada 5. Kerr AK, Farrar AM, Waddell LA, Wilkins W, Wilhelm BJ, Bucher O, Wills RW, Bailey RH, Varga C, McEwen SA, Rajic A. A systematic review-meta-analysis and meta-regression on the effect of selected competitive exclusion products on Salmonella spp. pervalence and concentration in broiler chickens. Prev Vet Med 2013; 111(1-2):11-125. 6. Kropinski AM, Waddell T, Meng J, Franklin K, Ackermann H-W, Ahmed R, Mazzocco A, Yates J III, Lingohr EJ, Johnson RP. The host-range, genomics and proteomics of Escherichia coli O157-H7 bacteriophage rV5. Virology J 2013; 10(1):76. 7. Levallois P, Chevalier P, Gingras S, Dery P, Payment P, Michel P, Rodriguez M. Risk of infectious gastroenteritis in young children living in Quebec rural areas with intensive animal farming: Results of a case-control study (2004-2007). Zoonoses Public Health 2013; Feb 14 (Epub ahead of print) 8. Marti R, Gannon VP, Jokinen C, Lanthier M, Lapen DR, Neumann NF, Ruecker NJ, Scott A, Wilkes G, Zhang Y, Topp E. Quantitative multi-year elucidation of fecal sources of waterborne pathogen contamination in the South Nation River basin using Bacterioidales microbial source tracking markers. Water Res 2013; 47(7):2315-2324. 9. Moreno Switt AI, Sulakvelidze A, Wiedmann M, Kropinski AM, Wishart DS, Poppe C, Liang Y. Salmonella phages and prophages - genomics, taxonomy and applied aspects. In: Salmonella Methods and Protocols (Methods in Molecular Biology), 2nd Edition. Editors: Schatten H, Eisenstark A. Book Chapter. 2014. 10. Pardhan-Ali A, Wilson J, Edge VL, Furgal C, Reid-Smith R, Santos M, McEwen SA. Community-level risk factors for notifiable gastrointestinal illness in the Northwest Territories, Canada, 1991-2008. BMC Public Health 2013; 13(1):63. 11. Parmley EJ, Pintar KDM, Majowicz S, Avery BP, Cook A, Jokinen CC, Gannon VPJ, Lapen DR, Topp E, Edge TA, Gilmour M, Pollari F, Reid-Smith RJ, Irwin RJ. A Canadian application of One Health: Integration of Salmonella data from various Canadian Surveillance Programs (2005-2010). Foodborne Pathog Dis 2013; 10(9):747-756. 12. Pouget JG, Coutinho FJ, Reid-Smith RJ, Boerlin P. Characterization of blaSHV genes on plasmids from E. coli and Salmonella of Canadian food animals (2006-2007). Appl Environm Microbiol 2013; 79(12):3864-3866. 13. Procter TD, Pearl DL, Finley RL, Leonard EK, Janecko N, Reid-Smith RJ, Weese JS, Peregrine AS, Sargeant JM. A cross-sectional study examining Campylobacter and other zoonotic enteric pathogens in dogs that frequent dog parks in three cities in South-Western Ontario and risk factors for shedding of Campylobacter spp. Zoonoses Public Health 2013; Jun 26 (Epub ahead of print) 14. Thomas MK, Murray R, Flockhart L, Pintar K, Pollari F, Fazil A, Nesbitt A, Marshall B. Estimates of the burden of foodborne illness in Canada for 30 specified pathogens and unspecified agents, circa 2006. Foodborne Pathogens and Disease 2013; 10(7):639-648. 15. Wilkes G, Ruecker NJ, Neumann NF, Gannon VPJ, Jokinen C, Sunohara M, Topp E, Pintar KD, Edge TA, Lapen DR. A spatiotemporal analysis of Cryptosporidium species/genotypes and relationships with other zoonotic pathogens in surface water from mixed use watersheds. App Environm Microbiol 2013; 79(2):434-448. 16. Drake M, Amadi V, Zieger U, Johnson R, Hariharan H. Prevalence of Salmonella spp. in cane toads (Bufo marinus) from Grenada, West Indies, and their antimicrobial susceptibility. Zoonoses Public Health 2013; 60(6):437-441. 17. Sylvester WR, Amadi V, Pinckney R, Macpherson CN, McKibben JS, Bruhl-Day R, Johnson R, Hariharan H. Prevalence, serovars and antimicrobial susceptibility of Salmonella spp. from wild and domestic green iguanas (Iguana iguana) in Grenada, West Indies. Zoonoses Public Health 2013; Dec 10 (Epub ahead of print). b) International conferences: 3 1. Yoshida C, Lingohr EJ, Card R, Trognitz F, MacLaren N, Rosano A, Villegas A, Polt M. OIE Reference Laboratory for Salmonellosis (CAN), Austrian Agency for Health and Safety, Salmonella Reference Laboratory (UK), and Kropinski AM. Multi-national laboratory comparison of a rapid Salmonella genoserotyping array (SGSA) and traditional serotyping for the identification of Salmonella serovars. American Society for Microbiology (ASM), 4th Conference on Salmonella: The Bacterium, the Host and the Environment, Boston, MA, October 5-9, 2013. Poster Presentation. 2. Yoshida C, Lingohr EJ, Laing CR, Kruczkiewicz, Gannon VPJ, Taboada EN. Salmonella In Silico Typing Resource
OIE Reference Laboratory Reports Activities, 2013
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Salmonellosis - Cornelis Poppe - canada (SISTR): rapid interpretation of Salmonella draft genome sequence data. American Society for Microbiology (ASM), 4th Conference on Salmonella: The Bacterium, the Host and the Environment, Boston, MA, October 5-9, 2013. Poster Presentation. 3.Muhammad RA, Ziebell K, Nash J, Laing C, Gannon V, Chui L, Gilmour M, Boerlin P, Devenish J, Johnson R. Development of a high resolution SNP-based typing tool for Salmonella Enteritidis. American Society for Microbiology (ASM), 4th Conference on Salmonella: The Bacterium, the Host and the Environment, Boston, MA, October 5-9, 2013. Poster Presentation. c) National conferences: 1 1. Yoshida C. Salmonella Genoserotyping Array (SGSA): Research Update and Implementation Plans. FoodNet, Guelph, ON. December 10, 2013. Oral Presentation. d) Other: (Provide website address or link to appropriate information) 1 1. Salmonella In Silico Typing Resource: lfz.corefacility.ca/sistr/
ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? No
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard? Yes
Quality management system adopted ISO 17025 16. Is your laboratory accredited by an international accreditation body? Yes
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Test for which your laboratory is accredited
Accreditation body
Serotyping of Salmonella isolates
Standards Council of Canada
Phagetyping of Salmonella Enteritidis, S. Heidelberg and S. Typhimurium
Standards Council of Canada
OIE Reference Laboratory Reports Activities, 2013
Salmonellosis - Cornelis Poppe - canada 17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? Yes (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
ToR: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE? No
19. Did your laboratory participate in scientific meetings on behalf of the OIE? No
ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Yes 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes
Purpose of the proficiency tests: 1
To develop and validate a Salmonella geno-serotyping array (SGSA) that rapidly generates antigenic formula consistent with the White-Kauffman-Le Minor Serotyping scheme 1
Role of your Reference Laboratory (organiser/ participant)
Organiser
No. participants
Participating OIE Ref. Labs/ organising OIE Ref. Lab.
2
Vet. Lab. Agency, Weybridge, UK, and the OIE Reference Laboratory for Salmonellosis, Guelph, Ontario, CANADA
validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific
OIE Reference Laboratory Reports Activities, 2013
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Salmonellosis - Cornelis Poppe - canada research projects for the diagnosis or control of the pathogen of interest? Yes
Title of the project or contract
Scope
Name(s) of relevant OIE Reference Laboratories
To develop and validate a Salmonella geno-serotyping array (SGSA) that rapidly generates antigenic formula consistent with the White-Kauffman-Le Minor serotyping scheme
To replace the laborious, costly and time-consuming Salmonella serotyping procedure with a rapid genoserotyping typing array
Vet. Lab. Agency, Weybridge, UK, and the OIE Reference Laboratory for Salmonellosis at Guelph, Ontario, CANADA
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Purpose for inter-laboratory test comparisons1
No. participating laboratories
Region(s) of participating OIE Member Countries
External Quality Assurance System (EQAS) for serotyping, phagetyping and determination of antimicrobial susceptibility of Salmonella strains, with other WHO Global Foodborne Infectious Network member laboratories
Laboratories participating in the EQAS of the WHO
Africa Americas Asia and Pacific Europe Middle East
3
Africa Americas Asia and Pacific Europe Middle East
2
Africa Americas Asia and Pacific Europe Middle East
Inter-Laboratory QA program regarding Salmonella serotyping and phagetyping with the Public Health Ontario (PHO), Enteric Laboratory, Toronto, Ontario, and with Laboratoire d`epidemiosurveillance animale du Quebec, MAPAQ, StHyacinthe, Quebec
Proficiency tests for phage-typing of Salmonella Enteritidis and Salmonella Typhimurium strains
8
OIE Reference Laboratory Reports Activities, 2013
Salmonellosis - Cornelis Poppe - canada
ToR: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE? Yes
Kind of consultancy
Location
Subject (facultative)
On request of the OIE Animal Production Food Safety Working Group and the Codex Alimentarius Commission reviewed a manuscript entitled A review of the scientific literature on the control of Salmonella spp. in food producing animals other than poultry (103 pages)and references.
OIE Ref. Laboratory for Salmonellosis in Guelph, Ontario, Canada
A review of the scientific literature on the control of Salmonella spp. in food producing animals other than poultry.
Review of manuscript upon request by the OIE for possible publication in the Scientific and Technical Review.
OIE Ref. Lab. for Salmonellosis in Guelph, Ontario, Canada
Impact of Salmonella enterica and international one-day-old poults trade in Egypt: carriage of virulence gene repertoire and their antimicrobial resistance profiles
Review of manuscript upon request by the OIE for possible publication in the Scientific and Technical Review.
OIE Ref. Lab. for Salmonellosis in Guelph, Ontario, Canada
Molecular epidemiology and characterization of Salmonella serovars from broilers reveals neglected concern of this zoonotic pathogen in Haryana, India.
25. Additional comments regarding your report:
OIE Reference Laboratory Reports Activities, 2013
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