Keith EJ Tyo Assistant Professor of Chemical & Biological Engineering Northwestern University 2145 Sheridan Rd. Tech E-136, Evanston, IL 60201 Tel: 847.868.0319 Email:
[email protected] tyolab.northwestern.edu
Education Massachusetts Institute of Technology Cambridge, MA Dept. of Chemical Engineering, Degree: PhD Advisor: Greg Stephanopoulos Minor: Systems Biology
2008
Dept. of Chemical Engineering, Degree: MS Chem. Eng. Practice West Virginia University Morgantown, WV Dept. of Chemical Engineering, Degree: BS Minor: Music
2003 2001
Professional Experience Northwestern University Evanston, IL Assistant Professor Co-director, Recombinant Protein Production Core Facility Member, Chemistry of Life Processes Institute Ass. Member, Robert H. Lurie Comprehensive Cancer Center Preceptor, Masters in Biotechnology Program (MBP) Advisor, MBP Sustainability & Global Health Certificate Manus Biosynthesis Cambridge, MA Scientific Advisory Board Consultant
2011 – Present
2011 - present
Chalmers University of Technology Göteborg, Sweden NIH NRSA Postdoctoral Fellow
2008 - 2010
XoutTB Cambridge, MA Scientist [Featured in The Economist, The Lancet, and Technology Review]
2007 – 2008
Cargill, Minneapolis, MN Chem. Eng. Practice Internship
2002
Masterfoods, Subsidiary of Mars, Reno, NV Chem. Eng. Practice Internship
2002
Dow Chemicals, Boundbrook, NJ Polymer Catalyst Intern
Summer 2000,2001
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Honors and Awards Awards since employment at Northwestern University NSF CAREER Award Early Career Award: Inst. for Sustainability and Energy at Northwestern Synthetic Biology Leadership Accelerator Program (SynBio LeAP), SynBERC Searle Scholars Teaching Program, Northwestern Searle Leadership Award, Northwestern
2015 2013 2012 2012 2010
Awards prior to employment at Northwestern University Wallenburg Foundation Travel Grant, Wallenburg Society 2010 FEMS Young Scientist Meeting Grant, Fed. Eur. Microbiol. Soc. 2009 Int. Conf. on Biomolecular Engineering 2nd prize Poster 2009 NIH F32 Kirschstein National Research Service Award Postdoctoral Fellow 2008 Wojtowicz Fellow (Best contribution to team, MIT Chem. Eng. Internship) 2003 Haslam Presidential Fellow (Outstanding 1st year PhD student, MIT) 2001 Summa Cum Laude, GPA = 4.0/4.0 (WVU) 2001 Barnes Award (Top engineering graduate, WVU) 2001 Presidential Scholarship (4 yr tuition award, WVU) 1997-2001
Teaching Experience Courses taught at Northwestern University ChE 210 Mass & Energy Balances ChE 275 Molecular & Cell Biology for Engineers ChE 373 Global Health & Biotechnology** ChE 478 Advances in Biotechnology Eng106-1 Design Thinking & Communication IBiS 455 Current Topics in Synthetic Biology * Co-taught at 50% effort **New course/ Curriculum development
AY 2013,2014,2015 AY 2011,2012*,2013* AY 2014,2016 AY 2015,2016 AY 2012,2013 AY 2013
Contributed Lectures at Northwestern University CE361 Public and Environmental Health AY2016 CE 448 Biophysicochemical Processes in Environmental Systems AY2014,2015 BME395 Global & Ecological Health Challenges AY2015,2016 BIO 215 Genetics & Mol. Biology AY 2012,2013 BIO 218 Biochemistry AY 2014 Chemical Biology (Statsyuk) AY2015,2016 BioExcel Program - Synthetic Biology AY 2012,2013,2014,2015
Researchers Supervised Ph.D. Students 1. Karthik Sekar Characterization of Metabolic Regulation in Non-growing Cells
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2011 – 2015
Keith EJ Tyo, PhD CV Last Updated: 11/28/16
2.
Matthew Moura (co-advised Linda Broadbelt) 2011 – 2015 Computational and Experimental Approaches to Design New Biosynthetic Pathways
3.
Dante Pertusi 2012 – 2016 Computational Tools for Characterizing and Predicting Enzyme Promiscuity
4.
Jennifer Greene (co-advised Linda Broadbelt) 2013 – Present Computational Tools for Characterizing and Predicting Enzyme Promiscuity
5.
James Jeffryes (co-advised Chris Henry) Enzyme Promiscuity Databases as Metabolic Maps
2013 – Present
6.
Adebola Adeniran Directed Evolution of G-protein Coupled Receptors
2013 – Present
7.
William Bothfield 2013 – Present Characterizing Metabolic Limitations in Non-growth through Metabolomics
8.
Alexandra de Paz Directed Evolution of DNA Polymerases for Novel Allosteric Sensing
9.
Sarah Stainbrook 2014 – Present Engineering Signaling G-coupled Protein Receptor Pathways for Diagnostics
10.
Soo Ro (co-advised with Amy Rosenzweig) Structural studies for methane monooxygenases
2014 – Present
11.
Bradley Biggs Engineering EF Hand Domains in DNA Polymerases
2016 – Present
12.
Peter Su (co-advised with Josh Leonard) Exploiting Split-proteins as Transcription Factors
2016 – Present
Visiting Professors 1. Nidhi Gupta Engineering Acinetobacter for biofuel production (professor at Jaypee Institute for Information Technology, India) Postdoctoral Fellows 2. William Rodriguez-Limas Increasing Protein Titers by Blocking Endocytosis (Currently at Ultra Laboratorios S.A. de C.V., Guadalajara, Mexico) 3.
Miaomin Zhang
2013 – Present
2016 - Present
2011 - 2012
2011 - 2014 -3-
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Characterizing Promiscious Reactions of Old Yellow Enzymes 4.
Jing Wu Rational Engineering of DNA Polymerase Processivity (Currently at Jiagnan University, Wuxi, China)
2013 - 2014
5.
Trang Vu Increasing Computational Performance of Cheminformatics Software
2013 - 2014
6.
Namita Bhan Engineering Deaminase-DNA Polymerase Fusions
7.
Jesse Collins Highthroughput Microfluidic Assays for DNA Polymerases
M.S. Students 1. Myles Hollenbach Engineering Non-growth Ethanol Production in Yeast (Currently at Genzyme, Framingham, MA) 2.
Michael Brotz Metabolic regulation in Non-growing E. coli
2014 - Present
2015 - 2016
2012 - 2014
2014 - 2015
Masters in Biotechnology Students 1. Harman Singh WebFBA – A User friendly webtool for estimating flux regulation
2011
2.
Yaelim Park Developing a high throughput screen for DNA polymerase activity (Currently Dental School student, Case Western University)
2012
3.
Patrick Ho (co-advised) Engineering a receptor dimer systems in yeast (Currently PhD student, UCLA Chemical Engineering)
2012
4.
Andrew Gentile Characterizing inducible protein degradation in E. coli
2013 - 2014
5.
Jonathan Ko (co-advised) WebFBA 2.0 – Expanding the utility of a flux balance analysis webtool
2013 - 2014
6.
Jeff Mayer (co-advised) Engineering a receptor dimer systems in yeast (Currently at Regeneron, Tarrytown, NY)
2013 - 2014
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7.
Divya Tikkavarapu Characterizing metabolic flux regulation in non-growing E. coli
2014
8.
Jessica Yu Optimization of directed evolution cycle of yeast receptors for low-cost diagnostics
2015
9.
Justin Newton Metabolomics on aromatic catabolizing soil bacteria
2016
Undergraduates (Undergrad research: S = spring; F = fall; W = winter; M = summer) 1. 2. 3.
4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
Michael Reddick S11,M11,F11,W11,S12,F12,W13,S13 PhD student at Stanford, NSF GRF Divya Venkat W12,S12,M12,F12,W13,F13,W14 Weinberg URG (declined), CLP Summer Scholars Fellow Michael Sherer W12,S12,M12,F12 Northwestern URG Med School at UC San Diego Victoria Tannenbaum S12,M12,F12,F13 Prog. In Biol. Sci. URG Grace Kapov M12,M13,W14,S14,M14 Northwestern URG Taylor Riley M13, F13, W14, S14 Stephen Lenzini F13,W14,S14 Isaac Yampolsky W14,S14,M14 HHMI NU Bioscientist Andrew Boston W14,S14 PhD at U of Colorado, Boulder Thomas Aunins M14,F14,S15,F15 Northwestern URG Aaron Anderson M15, F15,W16,S16 Christopher Coleman M15 Northwestern URAP Shanice Taylor W16,S16 Andrea Guerrero S16,M16 McCormick URG
2011 – 2013 2012 – 2014 2012
2012 – 2013 2012 – 2014 2013 – 2014 2013 – 2015 2014 – 2014 2014 2014 – 2016 2015 – Present 2015 2016 – Present 2016 – Present
Professional and Academic Leadership Organizing Conference Sessions Dumas Domain Dinner, Northwestern (co-host) Systems & Synthetic Biology
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2012
Keith EJ Tyo, PhD CV Last Updated: 11/28/16
Biochemical and Molecular Engineering XVIII Conference (session organizer) Advances in Low Cost Bioprocessing to Increase Access to Biotechnology
2013
Chicago Biomedical Consortium 12th Annual Consortium (co-organizer) Protein Engineering: From computers to cells to clinic
2014
Biochemical and Molecular Engineering XIX Conference (session organizer) Biorenewable Chemicals
2015
Metabolic Engineering 11 (session organizer) Design and Assembly of Metabolic Pathways
2016
Organizing Topical Journal Issues Guest Editor: Metabolic Engineering Journal Special Issue on Synthetic Biology Guest Editor: Current Opinions in Biotechnology Cell, Pathway, and Tissue Engineering Issue Program Development Certificate in Sustainability & Global Health Biotechnologies Masters in Biotechnology Program (MBP) Six students enrolled in two years.
2012
2014
2014 – Present
Professional Service Membership in Professional Societies American Institute of Chemical Engineering Society for Biological Engineering International Metabolic Engineering Society American Chemical Society Federation of European Microbiologist Society Session Chair and Conference Support American Chemical Society – Session chair American Institute of Chemical Engineering – Session chair(s) Biochemical and Molecular Engineering XVIII – Poster Judge Metabolic Engineering Conference – Poster Judge Grant Proposal Reviewer NSF CBET UK BBSRC (ad hoc) Vlaanderen Research Foundation – Flanders (Netherlands) Tufts University, Institute for Innovation Defense Threat Reduction Agency
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2001 - Present 2006 - Present 2013 - Present 2005 - Present 2009 - 2010
2012, 2016 2011 – 2015 2013 2014, 2016
2012, 2013, 2015 2014 2015 2015 2016
Keith EJ Tyo, PhD CV Last Updated: 11/28/16
Journal Reviewer ACS Synthetic Biology Applied Environmental Microbiology Applied Microbiology & Biotechnology Biochemical Engineering Journal Bioinformatics (Oxford) Bioprocess & Biosystems Engineering Biotechnology & Bioengineering Biotechnology & Bioprocess Engineering Biotechnology Journal BMC Genomics BMC Systems Biology Chemical Engineering Science Current Opinions in Biotech
FEMS Yeast Research Frontiers in Microbiology Journal of Biomedicine & Biotechnology Korean Journal of Chemical Engineering Metabolic Engineering Journal Microbial Cell Factories Nature Nature Biotechnology Nature Chemical Biology Nature Communications PLoS One Systems & Synthetic Biology
Outreach and Public Presentations Science Café Talk at Firehouse Grill Baker’s Yeast, Biotech, and Botswana: How cutting-edge biotech can help the global poor
2012
Chicago Area Undergraduate Research Symposium (CAURS) Keynote Speaker 2013 Grand Challenges in Human Health: Scientific discovery and engineering in the penicillin story (and beyond!) MIT Faculty Forum Online, Alumni Addition Genetic Engineering and CRISPR/Cas9 Panel
2016
Schuler Scholars mentor (Research opportunities for underprivileged high school/college from Waukegan, IL)
2016
Publications Submitted and In Revision 1.
Adeniran, A., Stainbrook, S., Tyo, K. “Engineering yeast G-protein coupled receptors as a healthcare diagnostic.” (in revision).
2.
Bothfeld, W., Kapov, G., Tyo, K. “A glucose-sensing toggle switch enables autonomous genetic controls that improve productivity.” (in revision).
3.
Pertusi, D., Moura, M., Jeffryes, J., Tyo, K. “Rapid characterization of enzyme promiscuity using active learning.” (submitted).
4.
Cybulksi, T., Boyden, E., Church, G., Tyo, K., Kording, K. “Nucleotide-Time Alignment for Molecular Recorders.” (submitted)
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5.
Stainbrook, S., Yu, J., Beghari, N., Tyo, K. “Modulating and evaluating receptor promiscuity through directed evolution and modeling.” (submitted)
6.
Wu, J., De Paz, A., Zamft, B., Marblestone, A., Boyden, E., Church, G., Kording, K., Tyo, K. “DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase.” (submitted).
Primary Research (accepted and published) 1.
Yu, J., Pertusi, D., Adeniran, A., Tyo, K. “CellSort: A support vector machine tool for optimizing fluorescence-activated cell sorting and reducing experimental effort.” Bioinformatics(Oxford) (accepted).
2.
Henry, C., Rotman, E., Lathem, W., Tyo, K., Hauser, M., Mandel, M. “Generation and validation of the iKp1289 metabolic model for Klebsiella pneumoniae” Journal of Infectious Disease (accepted).
3.
Wrenbeck, E., Klesmith, J., Stapleton, J., Adeniran A., Tyo, K., Whitehead, T. “Plasmid-based single-pot saturation mutagenesis.” Nature Methods (accepted)
4.
Ozer, E., Morris, A., Krapp, F., Henry, C., Tyo, K., Lathem, W., and Hauser, A.“Draft Genome Sequence of a Multi-Drug Resistant Klebsiella quasipneumoniae subsp. similipneumoniae Isolate from a Clinical Source.” Genome Announcements (accepted). Stine, A., Zhang M., Ro, S., Clendennen, S., Shelton, M., Tyo, K., Broadbelt, L. “Design and validation of de novo metabolic pathways from pyruvate to propionic acid.” Biotechnology Progress 32(2) 303-311 (2016).
5.
6.
Moura, M., Pertusi, D., Lenzini, S., Broadbelt, L., Tyo, K. “Characterizing and Predicting Carboxylic Acid Reductase Activity for Diversifying Bioaldehyde Production.” Biotechnology & Bioengineering 33 138-147 (2016).
7.
Sekar, K., Gentile, A., Bostick, J., Tyo, K. “N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.” PLoS One 11(2), e0149746 (2016).
8.
Moura, M., Finkle, J., Stainbrook, S., Greene, J., Broadbelt, L. J. & Tyo, K. E. Evaluating enzymatic synthesis of small molecule drugs. Metabolic Engineering 33, 138-147, (2016).
9.
Jeffreys, J., Colestani, R., Elbadawi-Sidhu, M., Kind, T., Niehaus, T., Broadbelt, L., Hanson, A., Fiehn, O., Tyo, K., Henry, C. “MINEs: Open access databases of computationally predicted enzyme promiscuity products for untargeted metabolomics.” Journal of Cheminformatic 7 44 (2015).
10.
Pertusi, D., Stine, A., Broadbelt, L., Tyo, K. A workflow for in silico design of long, novel, thermodynamically efficient metabolic pathways. Bioinformatics 31, 10161024 (2015).
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11.
Sekar, K., Tyo, K. Regulatory effects on central carbon metabolism from poly-3hydroxybutryate synthesis. Metabolic Engineering 28, 180-189 (2015).
12.
Rodriguez-Limas, W., Tannenbaum, V., Tyo, K. Blocking endocytotic mechanisms to improve heterologous protein titers in Saccharomyces cerevisiae. Biotechnology & Bioengineering 112(2), 376-385 (2015). *Biotech. & Bioeng “Spotlight” article: Taking yeast off its high protein diet. 112(2), iv (2015).
13.
Tyo, K. E., Liu, Z., Magnusson, Y., Petranovic, D. & Nielsen, J. Impact of protein uptake and degradation on recombinant protein secretion in yeast. Applied microbiology and biotechnology 98, 7149-7159 (2014).
14.
Glaser, J.I., Zamft B.M., Marblestone A.H., Moffitt J.R., Tyo K., Boyden E.S., Church G., and Kording K.P. Statistical analysis of molecular signal recording. PLoS computational biology 9, e1003145 (2013).
15.
Zamft, B.M., Marblestone A.H., Kording K., Schmidt D., Martin-Alarcon D., Tyo K., Boyden E.S., and Church G. Measuring cation dependent DNA polymerase fidelity landscapes by deep sequencing. PloS One 7, e43876, (2012).
16.
Tyo, K. E., Liu, Z., Petranovic, D. & Nielsen, J. Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress. BMC biology 10, 16, (2012).
17.
Liu, Z., Tyo, K. E., Martinez, J. L., Petranovic, D. & Nielsen, J. Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae. Biotechnology and bioengineering 109, 1259-1268, (2012).
18.
Hou, J., Tyo, K., Liu, Z., Petranovic, D. & Nielsen, J. Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae. Metab Eng 14, 120-127, (2012).
19.
Rodriguez-Limas, W. A., Tyo, K. E., Nielsen, J., Ramirez, O. T. & Palomares, L. A. Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae. Microbial cell factories 10, 33 (2011).
20.
Tyo, K. E., Fischer, C. R., Simeon, F. & Stephanopoulos, G. Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations. Metab Eng 12, 187-195 (2010).
21.
Ajikumar, P.K., Xiao W.H., Tyo K.E., Wang Y., Simeon F., Leonard E., Mucha O., Phon T.H., Pfeifer B., and Stephanopoulos G. Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli. Science 330, 70-74 (2010).
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22.
Tyo, K. E., Jin, Y. S., Espinoza, F. A. & Stephanopoulos, G. Identification of gene disruptions for increased poly-3-hydroxybutyrate accumulation in Synechocystis PCC 6803. Biotechnology progress 25, 1236-1243 (2009).
23.
Tyo, K. E., Ajikumar, P. K. & Stephanopoulos, G. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nature biotechnology 27, 760-765 (2009).
24.
Yu, H., Tyo, K., Alper, H., Klein-Marcuschamer, D. & Stephanopoulos, G. A highthroughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors. Biotechnology and bioengineering 101, 788-796 (2008).
25.
Tyo, K. E., Zhou, H. & Stephanopoulos, G. N. High-throughput screen for poly-3hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803. Applied and environmental microbiology 72, 3412-3417 (2006).
Reviews and Opinions (Peer Reviewed) 1. Adeniran, A., Sherer, M., Tyo, K. “Engineering Biosensing in Budding Yeast: A Review.” FEMS yeast research 15 (1), 1-15 (2014). 2.
Rodriguez-Limas, W. A., Sekar, K. & Tyo, K. E. Virus-like particles: the future of microbial factories and cell-free systems as platforms for vaccine development. Current opinion in biotechnology 24, 1089-1093 (2013).
3.
Hou, J., Tyo, K. E., Liu, Z., Petranovic, D. & Nielsen, J. Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae. FEMS yeast research 12, 491-510 (2012).
4.
Tyo, K. E., Kocharin, K. & Nielsen, J. Toward design-based engineering of industrial microbes. Current opinion in microbiology 13, 255-262, (2010).
5.
Petranovic, D., Tyo, K., Vemuri, G. N. & Nielsen, J. Prospects of yeast systems biology for human health: integrating lipid, protein and energy metabolism. FEMS yeast research 10, 1046-1059, (2010).
6.
Ajikumar PK, Tyo K, Carlsen S, Mucha O, Phon TH, Stephanopoulos G. Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms. Molecular pharmaceutics 5, 167-190 (2008).
7.
Tyo, K. E., Alper, H. S. & Stephanopoulos, G. N. Expanding the metabolic engineering toolbox: more options to engineer cells. Trends in biotechnology 25, 132-137 (2007).
Book Chapters and Editorials (Non-peer Reviewed)
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1.
Lütke-Eversloh T., Tyo K. Editorial overview: Cell and pathway engineering Moving from possible to profitable: recent innovations in cell and pathway engineering. Current Opinion in Biotechnology 29:v-vii (2014).
2.
Tyo, K. “Synthetic Biology for Global Health: A Problem-Driven Approach to Healthcare Innovation.” White paper. SynBio Leadership Accelerator Program (2013).
3.
Moura, M., Broadbelt, L. & Tyo, K. Computational tools for guided discovery and engineering of metabolic pathways. Methods in molecular biology 985, 123-147 (2013).
4.
Smolke, C. D. & Tyo, K. E. Synthetic biology: emerging methodologies to catalyze the metabolic engineering design cycle. Metab Eng 14, 187-188 (2012).
5.
Tyo, K. E., Nevoigt, E. & Stephanopoulos, G. Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation. Methods Enzymol 497, 135-155 (2011).
6.
Raab, R. M., Tyo, K. & Stephanopoulos, G. Metabolic engineering. Advances in biochemical engineering/biotechnology 100, 1-17 (2005).
Presentations Honorary and Invited Presentations and Lectures 1. International Conference for Biomolecular Engineering, San Diego, CA. Invited Speaker, January 2017. “Yeast-based biosensors as a low-cost healthcare diagnostic.” 2.
University of Wisconsin, Madison, Dept. of Chemical and Biological Engineering, Madison, WI. Seminar Speaker, Nov. 2016. “Yeast-based biosensors as a low-cost healthcare diagnostic.”
3.
Synthetic Biology, Engineering, Evolution, and Design (SEED), Chicago, IL. Invited Speaker, July 2016. “Yeast-based biosensors as a low-cost healthcare diagnostic.”
4.
Metabolic Engineering 11, Awaji Island, Japan. Invited Speaker, June 2016. “Understanding and Exploiting Enzyme Promiscuity for Metabolic Engineering.”
5.
University of Washington, Dept. of Bioengineering, Seattle, WA. Seminar Speaker, Jun. 2016 “Yeast-based biosensors as a low-cost healthcare diagnostic.”
6.
Tulane University, Dept. of Chemical & Biomolecular Engineering, New Orleans, LA. Seminar Speaker, Mar. 2016 “Yeast-based biosensors as a low-cost healthcare diagnostic.”
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7.
AIChE Annual Meeting, Symposium in Honor of Gregory Stephanopoulos 65th Birthday. Salt Lake City, UT. Invited Speaker, Nov. 2015 “Understanding and Exploiting Enzyme Promiscuity for Metabolic Engineering.”
8.
Medill Healthcare Reporting Conference. Washington, D.C. Invited Panelist, Oct. 2015. “Synthetic Biology: Innovations in Healthcare.”
9.
Biochemical & Molecular Engineering XIX, Puerta Vallerta, Mexico. Invited Speaker, July 2015. “Understanding and Exploiting Enzyme Promiscuity for Metabolic Engineering.”
10.
Northern Illinois University, Dept. of Chemistry & Biochemistry, DeKalb, IL. Seminar Speaker, Mar. 2015. “Cheminformatic Tools to Enabling Metabolic Synthesis of Novel Chemicals, Drugs, and Fuels.”
11.
Tufts University, Dept. of Chemical & Biological Engineering, Medford, MA. Seminar Speaker, Sept. 2014. “Cheminformatic Tools to Enabling Metabolic Synthesis of Novel Chemicals, Drugs, and Fuels.”
12.
Lanza Tech, Skokie, IL. Invited Speaker, Sept. 2014. “Tyo Lab Overview.”
13.
Univ. of Illinois, Urbana-Champaign, Energy Biosciences Institute, Champaign, IL. Invited Speaker, Aug 2014. “Cheminformatic Tools to Enabling Metabolic Synthesis of Novel Chemicals, Drugs, and Fuels.”
14.
University of Minnesota, Industrial Partnership for Research in Interfacial and Materials Engineering, St. Paul, MN. Invited Speaker, May 2014. “Cheminformatic Tools to Enabling Metabolic Synthesis of Novel Chemicals, Drugs, and Fuels.”
15.
Purdue University, Dept. of Biochemistry, W. Lafayette, IN. Seminar Speaker, Mar. 2014. “Cheminformatic Tools for Enabling Metabolic Synthesis of Novel Chemicals, Drugs, and Fuels.”
16.
Manus Biosynthesis Inc., Cambridge, MA. Invited Speaker, Dec. 2013. “Metabolites & Enzymes: Stoichiometry, Thermo, and Kinetics.”
17.
Midwest Yeast Meeting, Evanston, IL. Invited Speaker, Sept. 2013 “Increasing Protein Titers by Blocking Yeast Endocytosis and Vacuolar Degradation.”
18.
Shangdong University, State Key Laboratory of Microbial Technology, Jinan, China. Seminar Speaker, Jul. 2013 “Increasing Protein Titers by Blocking Yeast Endocytosis and Vacuolar Degradation.”
19.
Manus Biosynthesis Inc., Cambridge, MA. Invited Speaker, Aug 2012. “New Cellular Engineering Tools for New Metabolic Engineering Paradigms.”
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20.
Midwest AIChE Meeting, Chicago, IL. Invited Speaker. Nov. 2011. “Debottlenecking Metabolic Pathways to Increase Yield and Productivity.”
21.
Metabolic Engineering VIII Conference, Jeju, S. Korea. Invited Speaker. June 2010 “Chemically Inducible Chromosomal Evolution (CIChE): Increasing genetic stability by avoiding the pitfalls of plasmids.”
22.
Systems Biochemistry Conference, York, UK. Invited Speaker. Mar. 2010 “Protein Secretion in Saccharomyces cerevisiae: Mapping of Global Regulatory Structures.”
23.
Vanderbilt University, Dept. of Chemical and Biomolecular Engineering, Nashville, TN. Seminar Speaker. Feb. 2010 “Metabolic and Cellular Engineering: Next Generation Tools to Address Fundamental Challenges.”
24.
27th International Specialized Symposium on Yeast. Paris, France. Invited Speaker. August 2009. “Biological Networking of the Yeast Unfolded Protein Response.”
Invited Presentations at Northwestern University 1. Biotechnology Training Program, Northwestern University. Evanston, IL. Invited Speaker. Aug. 2014 “Increasing Protein Titers by Blocking Yeast Endocytosis and Vacuolar Degradation.” 2.
Dept. of Civil & Environmental Engineering. Northwestern University. Evanston, IL. Seminar Speaker. Oct. 2011. “Leveraging Synthetic Biology for Global Health.”
3.
Biotechnology Training Program, Northwestern University. Evanston, IL. Invited Speaker. Oct. 2011 “Leveraging Synthetic Biology for Global Health.”
4.
Interdepartmental Biological Sciences Program, Northwestern University. Evanston, IL. Retreat Speaker. Sept. 2011 “Leveraging Synthetic Biology for Global Health.”
5.
Dept. of Chemical and Biological Engineering, Northwestern University. Evanston, IL. Seminar Speaker. Feb. 2010 “Metabolic and Cellular Engineering: Next Generation Tools to Address Fundamental Challenges.”
Selectected Contributed Presentations 1. ACS Annual Meeting, San Diego, CA. Oral, Mar. 2016 “Understanding and exploiting enzyme promiscuity for metabolic engineering.” Pertusi, D., Moura, M., Jeffryes, J., Tyo, K., 2.
ACS Annual Meeting, San Diego, CA. Oral, Mar. 2016 “Directed evolution of yeast peptide receptors: A new platform for low-cost healthcare diagnostics.” Adeniran, A., Stainbrook, S., Tyo, K.,
3.
ACS Annual Meeting, San Diego, CA. Oral, Mar. 2016 “Detection of novel metabolites and enzyme functions through in silico expansion of metabolic
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models.” Jeffryes, J., Lerma-Ortiz, C., Cooper, A., Niehaus, T., Thamm, A., Frelin, O., Kind, T., Broadbelt, B., Fiehn, O., Hanson, A., Tyo, K. Henry, C. 4.
Synthetic Biology Engineering, Evolution & Design Conference, Manhattan Beach, CA, Poster, July 2014 “Yeast Receptor Evolution with a Novel High-Throughput Screen.” Adeniran, A., Bostick, J., Reddick, M., Pertusi, D., Sherer, M., Riley, T., Tyo, K.
5.
Metabolic Engineering X Conference, Vancouver, Canada. Poster, June 2014 “Computationally Guided Characterization of Carboxylic Acid Reductases for Expanding Aldehyde Bioproduction.” Moura, M., Lenzi, S., Pertusi, D., Tyo, K.
6.
Metabolic Engineering X Conference, Vancouver, Canada. Poster, June 2014 “Efficient searching and annotation of metabolic networks using chemical similarity.” Pertusi, D., Stine, A., Broadbelt, L., Tyo, K.
7.
AIChE Annual Meeting, San Francisco, CA. Oral, Nov. 2013 “Metabolic Pathway Inactivation Via Post Translational Modifications.” Sekar, K., Tyo, K.
8.
Midwest Yeast Meeting, Evanston, IL Poster, Sept. 2013 “High-throughput Screening of Yeast G-Protein Coupled Receptors.” Adeniran, A., Pertusi, D., Bostick, J., Tyo, K.
9.
Biochemical and Molecular Engineering XVIII Conference, Bejing, China. Poster, June 2013 “Increasing Protein Titers by Blocking Yeast Endocytosis and Vacuolar Degradation.” Rodriguez-Limas, W., Tannenbaum, V., Tyo, K.
10.
AIChE Annual Meeting, Pittsburgh, PA. Oral, Nov. 2012 “Protein endocytosis and degradation: Impact on secreted protein titers in yeast.” Tyo K., Rodriguez-Limas, W., Tannenbaum, V., Liu Z., Petranovic D., Nielsen, J.
11.
AIChE Annual Meeting, Pittsburgh, PA. Oral, Nov. 2012 “Flux regulation at a primary metabolic node: Lessons for acetyl-CoA derived products.” Sekar, K., Tyo, K.
12.
ACS Annual Meeting, San Diego, CA. Oral, Mar. 2012 “A Balancing Act in the Endoplasmic Reticulum: Relative Folding and Disulfide Bond Formation Rates are Key to Secretory Homeostasis.” Tyo, K., Liu Z., Petranovic D., Nielsen, J.
13.
35th Federation of European Biochemical Societies Congress, Göteborg, Sweden. Poster. June 2010 “Systems Biology of Protein Secretion in Saccharomyces cerevisiae: Mapping of Global Regulatory.” Tyo, K., Liu Z., Petranovic D., Nielsen, J.
14.
AIChE Annual Meeting, Nashville, TN. Oral, Nov. 2009 “Chemically Inducible Chromosomal Evolution (CIChE): Increasing Genetic Stability by Avoiding the Pitfalls of Plasmids.” Tyo, K., Ajikumar, P., Stephanopoulos, G.
15.
AIChE Annual Meeting, Nashville, TN. Oral, Nov. 2009 “Genetic and Metabolic Perturbation Analysis of the Polyhydroxybutyrate Pathway.” Tyo, K., Fischer, C., Stephanopoulos, G.
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16.
AIChE Annual Meeting, Nashville, TN. Poster, Nov. 2009 “Meeting in the Middle: Top-Down and Bottom-up Approaches to Metabolic Engineering.” Tyo, K., Nielsen, J., Stephanopoulos, G.
17.
27th International Specialized Symposium on Yeast. Paris, France. Poster, Aug. 2009. “Heterologous protein production in Saccharomyces cerevisiae.” Liu, Z., Tyo, K., Petranovic, D., Nielsen, J.
18.
Federation of European Microbiology Society 3rd Conference of European Microbiologists. Göteborg, Sweden. Poster. July 2009. “Recombinant Insulin Production by Saccharomyces cerevisiae.” Liu, Z., Tyo, K., Petranovic, D., Nielsen, J.
19.
International Conference on Biomolecular Engineering in Santa Barbara, CA. Poster. Jan. 2009 “Chemically Induced Chromosomal Evolution: A preferable alternative to plasmids for high copy, long-term, antibiotic-free expression.” Tyo, K., Ajikumaran, P., Stephanopoulos, G. [2nd place poster award]
20.
ACS Annual Meeting, Boston, MA. Speaker. Aug. 2007 “Growth-phase accumulation of poly-3-hydroxybutyrate in Escherichia coli: A prospectus for continuous production.” Tyo, K., Stephanopoulos, G.
21.
AIChE Annual Meeting, Cincinnati, OH. Speaker. Nov. 2005 “A High-Throughput Screen for Poly-3-hydroxybutyrate Accumulation for Inverse Metabolic Engineering.” Tyo, K., Zhou, H., Alper, H., Stephanopoulos, G.
22.
ACS Annual Meeting, San Diego, CA. Speaker. Mar. 2005. “Combinatorial methods for identifying gene knock-out targets for phenotypic improvement in Synechocystis PCC 6803.” Tyo, K., Stephanopoulos, G.
23.
Singapore-MIT Alliance Symposium, Singapore. Poster. Jan. 2005 “Inverse metabolic engineering of Synechocystis PCC 6803 for improved growth rate and poly-3-hydroxybutyrate production.” Tyo, K., Stephanopoulos, G.
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