BYI 02960 Matching Future Requirements with a New Molecule

2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 1

BYI 02960 – Brief Profile  Target Site:

Nicotinic Acetylcholine Receptor Agonist

 Major pests: Sucking Insects – Aphid, Leafhopper, Whitefly  Applications: Foliar, Soil, Seed  Systemicity: Xylem Limited  Global Joint Review Submission: Planned for mid-2012

Matching Future Requirements – with a New Molecule 2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 2

BYI 02960 – Research Code KEY MESSAGES

 Bee safety  Control of neonic resistant pests  Management of vectored diseases

Matching Future Requirements – with a New Molecule 2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 3

BYI 02960 – Pollinator Safety  Lab Toxicity Studies o Technical, metabolites and formulations o Acute, chronic and mechanistic o Honeybees, bumblebees and other pollinators

 Semi-field and Field Studies o Residues in pollen and nectar of representative crops o Effect trials • Mortality, foraging behavior and hive health • EUP planned to allow larger scale studies

 Lab and semi-field studies completed to date demonstrate that BYI 02960 is safe to bees Positive pollinator safety profile to demonstrating pollinator safety Significant resources committed 2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 4

BYI 02960 – Profile – Foliar & Soil Average of field data 2007-2009

BYI 02960

Imidacloprid

Aphis sp.

++++

+++

Myzus sp.

+++

++++

Dysaphis plantaginea.

++++

++++

Empoasca sp.

++++

+++

+++

++++

Rice hoppers (GLH, WBPH).

++++

++++

Bemisia sp.

++++

++ / -

Trialeurodes vaporariorum

+++

+++

Aphids

e.g. Aphis sp., Myzus sp

+++

++++

Hoppers

e.g. BPH, SBPH, WBPH

+++

+++

Whiteflies

e.g. Bemisia sp.

++++

+++

Aphids

Foliar

Hoppers

Nilaparvata lugens (BPH)

Whiteflies

Soil

Spectrum extension: mealybugs, flea beetles, certain thrips, Citricola scale, BBM, etc.

Confirming activity against key pests 2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 5

BYI 02960 – SL200 – Foliar

Apple – Dysaphis plantaginae (Pre- bloom, Early pink)

Trial ID: ID09NARA6M-UTY1, Jim Steffel, New Smithfield, PA – A = 2009-04-29 BYI 02960 plus NO SURFACTANT

Average RAA on a 0-5 scale

Rosy Apple Aphid count 5/22/09 4

23 DAA 3

Highest number of aphids counted for ten colonies per tree with 4 trees per treatment. Aphids on a 0-5 scale where 0 = no ahids; 1 = 1-3 aphids; 2= 4-10 aphids; 3 = 11-30 aphids; 4 = 31-100; 5 =>100 aphids

3 3 2 2 1 1 0

0 Control

2009-12-06 • Slide 6

Calypso 480 SC 4oz/a

0

BY102960 3.42oz/a

0

0

0

BY102960 6.84oz/a

BY102960 10.26oz/a

Calypso SC250 7.7oz/a

BYI 02960 – SL200 – Foliar

Citrus – Coccus pseudomagnoliarum (Nymphs) – Citricola scale Trial ID: ID10NARA6T-USQ1, Sam Wells, Fresno, CA – A = 2010-06-22 – 1075 liters/Ha Effect (% Abbott)

No Nymphs/Leaf

All Treatments plus Dyne-Amic @ 0.25% v/v

Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)

100

b 80

b

b

b

b

b

b

300

b

b b

b

b b

240

b

b

60

180

a a a

40

20

b

120

60

a

0

0 2 DAA

8 DAA

15 DAA

BYI 02960 SL200 21884 - 100

BYI 02960 SL200 21884 - 150

PROVADO 192SC 100

UNTREATED

2009-12-06 • Slide 7

rate g ai/ha 3.4 meter trees Protocol ID10NARA6T

22 DAA

59 DAA

BYI 02960 SL200 21884 - 200

No PTX

BYI 02960 – Citrus – Citricola Scale Damage Citrus – Coccus pseudomagnoliarum (Honeydew) – Citricola scale Trial ID: ID10NARA6T-USQ1, Sam Wells, Fresno, CA – A = 2010-06-22 – 1075 liters/Ha

Untreated

2009-12-06 • Slide 8

BYI 02960 (150 gms A/HA)

Protocol ID10NARA6T

BYI 02960 – SL200 – Foliar

Blueberry – Fimbriaphis fimbriata (Mixed) – Blueberry aphid

Trial ID: ID09NARADL-JAD3, Andrew Dornan (McMillan, ICMS - BC), Abbotsford, BC – A = 2009-07-10 – 500 lts/Ha Effect (% Abbott)

No of insects / marked branch Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls.)

100

b

b

b

b

b

b b

b

b

b

b

b

b

b

b

b

b

b

b

20

b

80

16

60

12

40

8

20

a

a

a

4

a 0

0 3 DAA

5 DAA

7 DAA

14 DAA

BYI 02960 21884 – 25

BYI 02960 21884 – 50

BYI 02960 21884 – 100

BYI 02960 21884 – 50 + Agral 90 0.5% v/v

Admire 2F – 42

Untreated Check rate g ai/ha

2010-08-20 • Slide 9

Protocol ID09NARADL

No PTX

BYI 02960 – SL200 – Foliar

Cantaloupe – CYSDV – Cucurbit Yellow Stunting Disorder Virus

Trial ID: ID09NARA6C-UMW1, Dr. John Palumbo, Yuma, AZ – A = 2009-06-01, B = 2009-06-15 - 191.7 lts/Ha Effect – Leaves YIVC*/row-ft

All Treatments plus Dyne-Amic @ 0.25% v/v Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)

5

4

3

a a ab

2

ab

ab

bc bc

1

cd d

d

ab

bc

bc

bc c

cd

bc

c

0 14 DAB / 28 DAA

19 DAB / 33 DAA

UNTREATED

BYI 02960 SL200-21884 - 100

BYI 02960 SL200-21884 - 150

BYI 02960 SL200-21884 - 200

Venom 70SG - 196

MOVENTO SC240 - 88

Fulfill 50WDG - 96

Fulfill 50WDG - 96 + ActiGard

Assail 30SG - 112

rate g ai/ha 2010-03-19 • Slide 10

Protocol ID09NARA6C

*YIVC = Yellows, interveinal chlorosis

No PTX

BYI 02960 – nAChR Resistance Cross-Resistance to Neonicotinoids  NN resistance is primarily metabolic  NN resistance not target-site  BYI 02960 not degraded through equivalent pathway

2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 11

BYI 02960 – SL200 – Soil

Squash – Bemisia tabaci (Silverleafing) – Sweetpotato whitefly Trial ID: ID10NARA6H-UJU2, John Curtis, Dover, FL – A = 2010-08-09 (soil, drip) Effect (% Abbott)

Number “Silvered” Leaves/10 Plants Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)

100

b b b b b

b b b b b

c

c c

c

c

c

500

c

d

c 80

b

d

d

d 400

c c b

60

cd a

a

300

c a

40

b

a 20

200

b 100

a a

0

0 7 DAA

14 DAA

18 DAA

21 DAA

25 DAA

29 DAA

BYI 02960 SL200 21884 - 200

BYI 02960 SL200 21884 - 300

BYI 02960 SL200 21884 - 400

ADMIRE PRO 550SC - 424.6

VENOM 70WG - 400

UNTREATED

rate g ai/ha 2010-09-08 • Slide 12

Protocol ID10NARA6H

No PTX

B

A

ID10NARA6HUJU2 (BEMIAR/DRIP/SQUASH): Silverleafing in BYI 02960 @ 9/23/2010 400 G A/HA (A, 89.2% control), compared with the untreated buffer (B) at 29 DAA.

BYI 02960 – NN-resistant Populations

NN-resistant Whiteflies

NN-resistant Hoppers

Bemisia argentifolii, Brazil Bemisia tabaci, Spain Bemisia tabaci Q, Japan Bemisia tabaci B, US BPH, 2006 BPH, 2007 BPH, 2008 BPH, 2009

BYI 02960

Imidacloprid

+++

+

++++

+

+++

-

+++

+

+++

+

+++

+

+++

-

+++

-

Tool for resistance management programs 2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 14

Thank You!

2010-09-15 • BYI 02960 Introduction IR-4 FUW • Slide 15