Ass. Prof. Katarzyna Borowska

In my scietific research , there was also the opportunity for clinical research dealing with the role of Demodex in etiopathogenesis of rosacea. I conducted this research as an assitant at the Department of Dermatology of the Military Institute of Health under the supervision of Professor Dr. Stanislaw Leopold Zabielski. The dependance between the coexistence of Demodex and rosacea is not completely clear. There is no clear accepted standard procedures for ethiopatogenesis. According to the results of my research, since patients positively reacted to the antiparasitic treatment, further research should be done on Demodex. This is research is especially helpful for patients with advanced and chronic rosacea. With such patients, this treatment should be administrated on a regular established basis. The results and analysis were published in academic journlas or presented at academic conferences as follows: Borowska K., Antiszko M., Zabielski S. Ethiopathogenesis of rosacea with special attation to Demodex mite. Lekarz Wojskowy, 2003, 79, nr 4, 235-238. Borowska K., Antiszko M., Zabielski S. Ethiopathogenesis of rosacea with special attation to Demodex mite. . XXXVIII Konferencja Naukowo-Szkoleniowa Dermatologów Wojska Polskiego, 30-31.05.2003, Kazimierz Dolny. Borowska K., Antiszko M., Zabielski S. Demodecosis – case report. Posiedzenie Polskiego Towarzystwa Dermatologicznego, luty 2004, Centrum Konferencyjne Wojska Polskiego Warszawa. Antiszko M., Borowska K., Galus R., Zabielski. Współczesne poglądy na etiopatogenezę i leczenie trądziku różowatego XIII Konferencja Pediatrów i Lekarzy Medycyny Rodzinnej - Aktualne standardy diagnostyki i leczenia w pediatrii oraz medycynie rodzinnej. 1619.03.2006, Zakopane. As an assistant in Military Institute of the Health Service in Warsaw, Department of Dermatology Central Clinical Hospital of the Ministry of National Defence I started my collaboration with Leszek Wdowiak, the Professor of Medicine and the chairman of Public Health Department at Medical University in Lublin. The aim of our cooperation was the analysis of the marketing tools in the assessment of dermatology services. Dermatological diseases, due to their prevalence as well as social importance, are considered to be a significant problem of public health. A correctly executed analysis and decent management of the dermatological services sector are particularly important in the area of public health medicine. In the same thematic group there are my works that concern the tools used in the assessment of life quality of people with skin diseases. In order to objectify the influence of diseases, medical questionnaires should be implemented into both clinical and ambulatory studies. Such questionnaires in clinical practice were used to assess the quality of life among the patients in Military Institute of the Health Service in Warsaw, Department of Dermatology Central Clinical Hospital of the Ministry of National Defence. The results confirm that skin diseases should be considered no only in medical aspects, but also in psychosocial. On the basis of the collected material, several scientific publications and presentation were published with my authorship and co-authorship.

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Borowska K. Wdowiak L Ortyl G. Przydatność narzędzi marketingowych w ocenie usług sektora dermatologicznego. Zdrowie Publiczne, 2004, 114, nr 3, 429-432. Wdowiak L., Borowska K., Szymańska J. Measurement tools of the quality of life in patient with skin illnesses. Zdrowie Publiczne, 2004, 114, nr 4, 575-580. Wdowiak L,, Chrzanowska D., Borowska K., i wsp. Acne vulgaris as a social-medical problem. Zdrowie Publiczne, 2004, 114, nr 3, 400 – 404. Borowska K. Wdowiak L. Analiza strategiczna sektora dermatologiczneg. Dni Medycyny Społecznej i Zdrowia Publicznego, 23-24.09.2004. Zielona Góra. Borowska K. Wdowiak L. Analiza SWOT oraz Macierz BCG w ocenie usług sektora dermatologicznego. Dni Medycyny Społecznej i Zdrowia Publicznego, 23-24.09.2004. Zielona Góra. XXVIII Zjazd Polskiego Towarzystwa Dermatologicznego, Bydgoszcz, 0105.09.2004. An important element of my work was participation in research on the use of CO2 laser in treating skin cancers Carcinoma in situ – Bowen's disease and its variety - erythroplasia of Queyrat. The research was conducted within the framework of the project called „Early detection and treatment of precancerous and cancerous conditions of skin (2001)” under the supervision of Stanisław Leopold Zabielski, the Professor of Medicine. In the research I collaborated with Adam Borzęcki, Doctor of Medicine, who is the head of a hospital ward of the Dermatology Ward NZOZ Med-Laser in Lublin. In the research I was the co-executor. The laser influence on the operated tissue is determined by the time effects and the wavelenght of the laser beam, which may variously influence the tissue, depending on the absorbency level. During the surgery, the laser beam may be absorbed, reflected or diffused by the tissue. The curative effect is evoked only in the first one. The surgery takes place under local anaesthetic, in the ambulatory conditions. As the academic studies suggest, the therapeutic effect as well as the use of CO2 laser in the clinical settings depend on the size and the location of the cutaneous condition. Basing on the previous studies it was concluded that the CO2 laser may be safely and successfully aplied to Bowen's disease and erythroplasia of Queyrat. The laser in an alternative to the conventional surgery methods. The patients recover quickly. Postsurgery complications are very rare, whereas the outcomes very good. The presentation and the study cases are presented in the following works: Borowska K., Zabielski S. Erythroplasia of Queyrat- clinical manifestations, some methods of treatment. Nowa Medycyna 2005, nr 2, 127, 42-44. Borowska K., Zabielski S. Morbus Bowen – clinical and histological differentiation. Nowa Medycyna 2005, nr 2, 127, 39-41. Borowska K., Zabielski S. Współczesne metody leczenia choroby Bowena. XXXIX Konferencja Naukowo-Szkoleniowa Dermatologów Wojska Polskiego, 04-05.06.2004, Polanica Zdrój. Borowska K. Borzęcki A., Zabielski S. Bowen's disease treated with carbon dioxide laser case report XXVIII Zjazd Polskiego Towarzystwa Dermatologicznego, 01-05.09.2004, Bydgoszcz, Przegląd Dermatologiczny 2004, 91, 304.

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Borowska K. Borzęcki A., Zabielski S. Erythroplasia of Queyrat treated with carbon dioxide laser. 9 Konferencja Naukowo-Szkoleniowa Sekcji Dermatochirurgii Polskiego Towarzystwa Dermatologicznego. 7-9 grudnia 2006 Szczyrk. Yet another undertaking I took part in was connected with conducting the mycology studies within the framework of the project started in 2001 „The prevention of foot and nail mycosis among of soldiers of military service”. Basing on the gathered materials, many analyses have been performed in order to implement the mycosis prevention among soldiers. The supervisor of the project was Stanisław Leopold Zabielski, the Professor of Medicine, the chief Dermatology Consultant of the Polish Army and the head of Department of Dermatology Military Institute of Health, Warsaw and I served as a coexecuter of the research. Some of the important publications of my authorship are cited in the following positions: Antiszko M., Borowska K., Galus R., Zabielski S. Etiopathogenesis and epidemiology of foot mycosis. Pediatria Medycyna Rodzinna, 2005, vol. 1, nr 4, s. 255-260. Borowska K., Antiszko M., Zabielski S., Galus R. Efficacy and safety profile of treatment of superficial fungal infections. Pediatria, Medycyna Rodzinna, 2006, vol. 2 nr 1, s. 38-43. Borowska K., Zabielski S. Profilaktyka zakażeń grzybiczych skóry. Lekarz Wojskowy, 2007, 83(3), 179-181. In 2006 I created some review works from the area of integrin. Integrins constitute numerous and diverse group of adhesion molecules. Their mail role is cell adhesion. The aim of the work was to transmit and systematize integrins contribution in physiological and pathological processes (inflammatory and cancerous). The works were created as a part of cooperation between Chair and Department of Histology and Embryology Medical University in Lublin and Department of Dermatology in Military Institute of Health in Central Clinical Hospital of the Ministry of National Defence in Warsaw. Borowska K., Jędrych B., Czerny K, Zabielski S. The role of integrins in the pathogenesis of inflammatory processes. International Review of Allergology and Clinical Immunology, 2006, Vol. 12, No. 3, 109-114. Borowska K., Jędrych B., Czerny K, Zabielski S. The role of integrins in neoplastic processes. International Review of Allergology and Clinical Immunology, 2006, Vol. 12, No. 3, 115-119. Borowska K., Jędrych B., Czerny K, Zabielski S. The role of integrins in the physiologic and pathogenic processes. Polski Merkuriusz Lekarski, 2006, XXI, 124, 362-366. An important element of my academic performance was participation in a project designed for the Polish Army, concenring the estimation of skin sensitivity of the Polish soldiers to broadband UVB irradiation. Stanisław Leopold Zabielski, the Professor of Medicine, was in charge of the project, whereas I was a coordinator. I gained the experience in the area of skin photodiagnostic when I was a coordinator of Photobiology Laboratory in Department of Dermatology in Military Institute of Health in Central Clinical Hospital of the Ministry of National Defence in Warsaw. There were 130 patients (soldiers) of the 5th Polish contingent of the army (Iraq) who took part in the research. They were preparing for the peacekeeping mission in the areas of strong UV radiation. The UV exposion may contribute to dangerous

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and irreversible complications, such as photocarcinogenesis. The risk factors, on which the soldiers working in countries of strong UV radiation were exposed to, were analyzed. Basing on the gathered material, many analyses were conducted in order to determine the minimal erythema dose (MED). The wide decomposition of MED was observed with the slip to the higher values of MED. Among the soldiers who previously were on peacekeeping missions in the areas of high UV radiation, the higher MED values were observed in comparison to the other participants not staying in such climate zones. These results are particularly important to the army when it comes to preventive actions and the further strategy. Conducting the research in order to estimate MED on numerous groups (soldiers) gave the opportunity for new results, not described before. The results of the analyses were published in Lekarz Wojskowy and presented as a presentation during Young Dermatologists Forum. Borowska K., Zabielski K.: The Evaluation of Skin Sensitivity to Broad-band UVB Irradiation – on the example of Polish soldiers serving in the International Forces in Iraq. Lekarz Wojskowy, 2006; 82 (3): 181-184. Borowska K. Zabielski S. Ocena wrażliwości skóry na szeroki zakres promieniowania UVB Forum Młodych Dermatologów Polskiego Towarzystwa Dermatologicznego 1-2 październik 2005, Uniwersytet Mikołaja Kopernika w Toruniu. Streszczenie: Dermatologia Kliniczna 2005, 7, 4, 13 My academic research also includes the results of clinical studies. I presented rare and unusual cases of patients at numerous academic gatherings but mostly at the Polish Society of Dermatology in Warsaw. Szczecińska W. Kozłowska A., Czarnecka I. Borowska K. Mycosis fungoides, Mucinosis follicularis – obserwacja. Posiedzenie Polskiego Towarzystwa Dermatologicznego 10.11.2004 Warszawa. Borowska K. Ziemlewski K. Pemphigoid prezentacja przypadku. Posiedzenie Towarzystwa Dermatologicznego 15.02.2006 Centrum Konferencyjne Wojska Warszawa. Borowska K. Zapalenie skórno-mięśniowe prezentacja przypadku. Posiedzenie Towarzystwa Dermatologicznego 14.02.2007 Centrum Konferencyjne Wojska Warszawa.

Polskiego Polskiego Polskiego Polskiego

My research includes original work about the 2-chlorodeoxyadenozyny (2CDA). 2CDA is a purine analoge used in treating Hairy Cell Leukemia (HLD) and Sclerosis Muliplex (SM). The aim of study was to assess histomorphologically the skin of experimantal animals that were given 2CDA. The drug was administered subcutaneously. There were two experimental groups and one control group. In the first experimental group, the study material in the form of a full thickness skin sample was taken 24 hours after the last 2CDA dose. In order to evaluate the regeneration abilities of the skin, the study material was taken from the second group of experimental animals 4 weeks after the last dose fo the drug was administered. Subcutaneous administration of 2CDA to the first group of experimental animals resulted in histological changes in the material studied. The findings obtained from skin tests in the second experimental group proved that the skin morphology had not returned to the basaline condition and the further histological changed had occurred. The results were published in the Polish Journal of Environmental Studies and presented at the Second International Scientific Symposium in Kazimierz Dolny.

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Jędrych B., Borowska K., Kifer E., Matysiak W., Czerny K., Zabielski S. Histomorphologic Assessment of Experimental Animals’ Skin in Terms of 2-CDA Activity at Doses used in Treating Lymphatic System Proliferative Diseases. Polish Journal of Environmental Studies, 2007, Vol.16, No 5C, Part I, 2007, 197-200. Jędrych B., Borowska K., Budzyńska B., Kifer E., Matysiak W., Czerny K., Zabielski S. 2CdA influence on skin histomorphology in experimental animals treated with doses used in multiple sclerosis therapeutic trials. Polish Journal of Environmental Studies, 2007, Vol.16, No. 6D, 219-222. Jędrych B., Borowska K., Kifer E., Matysiak W., Czerny K., Zabielski S. Histomorphologic assessment of experimental animals' skin in terms of 2-CdA activity at doses used in treating lymphatic system proliferative diseases. (Wpływ 2-CdA na histomorfologię skóry zwierząt doświadczalnych po podaniu dawek stosowanych w leczeniu białaczek.) W: II Międzynarodowe Sympozjum Naukowe "Środowiskowe źródła zagrożeń zdrowotnych". Kazimierz Dolny, 26-28 kwietnia 2007. [Streszcz.] T. 2, s. 526.

Jędrych B., Borowska K., Budzyńska B., Kifer E., Matysiak W., Czerny K., Zabielski S. 2007. 2-CdA influence on skin histomorphology in experimental animals treated with doses used in multiple sclerosis therapeutic trials. (Wpływ 2-CdA na histomorfologię skóry zwierząt po podaniu dawek stosowanych w próbach leczenia stwardnienia rozsianego.) W: II Międzynarodowe Sympozjum Naukowe "Środowiskowe źródła zagrożeń zdrowotnych". Kazimierz Dolny, 26-28 kwietnia 2007. [Streszcz.] T. 2, s. 528. Taking advantage of my experience in the area of photodermatology and photobiology and histology, I started my cooperation with various scientific institutions. Collaborative publications and research topics are the effects of this cooperation. Working with Chair and Department of Histology and Embriology, Center for Biostructure, Medical University of Warsaw I was the coauthor of the review paper concerning the influence of UV radiation on skin as well as I participated in oroginal research concerning the influence of statins on melanogenesis. The influence of fluvastatin on the process of melanin synthesis as a protective activity against UV radiation was analyzed. Fluvastatin inhibit cholesterol synthesis by reducing 3-hydroxy-3-glutaryl-coenzyme a (HMG-CoA) reductase activity. The role of fluvastatin was examined in potential modulation of thyrosinase (key enzyme of melanogenesis) synthesis. All experiments were performed using B16F10 melanoma cells. Cells were irradiated 100mJ/cm². In the current study we show that fluvastatin increases tyrosinase synthesis induced by UVB irradiation of B16F10 melanoma cells. According to the above results, fluvastatin treatment may potentially increase melanin synthesis and skin protection against UV irradiation. In 2009 the results were published in Folia Histochemica et Cytobiologica, and on October 24th 2001 the work was honoured by the Rector of Medical University of Warsaw Marek Krawczyk, The Professor of Medicine. The team prize of the 3rd degree was awarded. Galus R., Zandecki Ł., Antiszko M., Borowska K., Zabielski S. Fotostarzenie się skóry. Polski Merkuriusz Lekarski. 2007, XXII, 132, 580-584.X Fotostarzeę skóry

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Galus R, Sajjad E, Niderla J, Borowska K, Włodarski K, Włodarski P, Jóźwiak J. Fluvastatin increases tyrosinase synthesis induced by UVB irradiation of B16F10 melanoma cells. Folia Histochem Cytobiol. 2009 Jan;47(3):363-5. Borowska K. Nagroda zespołowa naukowa trzeciego stopnia Rektora Warszawskiego Uniwersytetu Medycznego Prof. dr hab. n. med. Marka Krawczyka za współautorstwo pracy dotyczącej wpływu statyn na melanogenezę. Warszawa 24 październik 2011. Taking advantage of the gained experience in mycology during the previously mentioned project „The prevention of foot and nail mycosis among of soldiers of military service” I developed my interests in mycosis prevention among sportsmen. The works were published as separate chapters in „Medycyna Sportowa”, edited by Krzysztof Klukowski, the Professor of Medicine. Borowska K. Profilaktyka zakażeń grzybiczych u sportowców. W: Medycyna sportowa. Wydaw: Medical Tribune Polska. Warszawa 2011, wyd.1. s. 178-182. Borowska K. Farmakoterapia grzybicy powierzchownej. W: Medycyna sportowa. Wydaw: Medical Tribune Polska. Warszawa 2011, wyd.1. s. 183-187.

Numerous presentations and publications that were results of my clinical experience were yet another important element of my work. I presented interesting and rare cases of dermatological diseases during scientific conferences. Many of these publications are presented in „Leksykon Dermatologiczny”, published in 2011. Borowska K. Borzęcki A. Zabielski S. Extrammamry Paget's disease treated by reated with carbon dioxide laser and surgery. X Jubileuszowa Konferencja Naukowo-Szkoleniowa Sekcji Dermatochirurgii PTD. 20-23. 09. 2007 Jurata. Wasyłyszyn T. Borowska K. : Program edukacyjny - dermatologia w praktyce. Dermatologia i kosmetologia Praktyczna 2008 Dec 4 (12): 59-61. Borowska K, Borzęcki A., Ziemlewski K. Leczenie zmian barwnikowych w zespole PeutzaJeghersa przy użyciu lasera CO2 oraz IPL - prezentacja przypadku. Dermatologia Estetyczna. Nr 1 (66)/styczeń-luty 2010. Borzęcki A., Borowska K., Szponar A. Melanoacanthoma skóry – prezentacja przypadku, przegląd literatury. Dermatologia Kliniczna 2010, 12 (2): 114-116. Wasyłyszyn T, Borowska K. : Program edukacyjny - dermatologia w praktyce. Dermatologia i kosmetologia Praktyczna 2011 Mar. Vol. 6 Nr 1 (21) : 59-61.

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