Training Courses www.crawfordscientific.com

Ov er v i ew Training from Crawford Scientific The requirement for consistent high standards of competence in chromatography and analytical science is now, more than ever, a matter of priority for pharmaceutical, chemical and environmental organizations throughout the world. Crawford Scientific are a recognised provider of high quality, flexible training in analytical science designed to provide maximum impact on student knowledge and effectiveness in the laboratory. Our training events are lively and enjoyable whilst ensuring that every attendee is developed to their maximum potential using modern laboratory instrumentation and cutting edge, but practically relevant, theory. Crawford Scientific employs highly skilled and experienced trainers to plan and implement training events that give optimum practical impact.

Our Promise We aspire to excellence in the following areas: • • • • • • • •

The knowledge and professionalism you will receive from our training staff The practical usefulness and effectiveness of the training we provide to you The quality of training manuals and printed materials for your retention The quality and effectiveness of our training delivery The post-course support and assistance that you can expect The provision of assessments and other training metrics for you to measure Return on Investment The support you can expect from extensive web-based resources and e-learning The administrative processes that we operate to help you book, implement and follow up on training events

Customer feedback is not something we take for granted. We provide extensive post course support for all our attendees, and are constantly updating our training material to reflect advances in technology and practice.

Book online: www.crawfordscientific.com/training-online-calendar.asp

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“I would not hesitate to use Crawford for future training.”

F eedbac k

“I was very pleased with the “can-do” attitude to training that Crawford displayed.”

“This was the best course I have attended. Very relevant & focused.”

Delivery Options We provide a wide range of training solutions from client specific customised courses to off-site seminars in various locations throughout the UK. Our events are designed to match your delegate numbers, training requirements and budgets. We offer: • • •

On-site events Off-Site Practical Courses Off-Site Classroom Courses

Our Clients Feedback We are proud to be associated with a wide variety of UK clients ranging from international blue chip corporations and government organisations to small start-up companies. As such we promise that we have the experience to engage with you to provide an effective training event for your staff. We ensure that everyone attending our training events has a participative experience and advances their knowledge or experience to the maximum extent possible. You can see examples of feedback from our clients throughout our brochure and shown below is a summary of client feedback we have received over the past 15 years.

On-Site Course Feedback Statistics 9.8

9.5

10.0

9.3

9.2

8.9

9.1

Off-Site & Practical Course Feedback Statistics 9.3

9.1

10.0

9.6

9.5

9.5

9.0

9.0

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8.4

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8.7

Further information contact: [email protected]

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Off- Si te Tr ai ni ng

Off-Site Practical Training If your number of delegates is small or if you want maximum training impact away from the distractions of the workplace, then off-site practical training is the ideal choice. These courses are designed to suit delegates of all levels of experience. The practical course content is not specific to any particular instrument manufacturer but will emphasise the important principles behind instrumental analysis. Run at our laboratory facilities in Manchester and London, these hands-on courses are designed to introduce both theory and practical concepts of instrumental analysis. Modern equipment is used in conjunction with interactive teaching techniques and well established practical exercises to ensure maximum return on your training investment There are only SIX places on each of these courses to allow maximum training impact – very early booking is advised as these courses are very popular indeed.

Practical Course Titles

Practical Course Venues

• • • • • • • •

• •

Practical LC-MS/MS (2 Days) HPLC Troubleshooting & Maintenance (2 days) HPLC Method Development (2 days) GC Troubleshooting & Maintenance (2 Days) Practical GC-MS (2 days) GC Method Development (2 days) Fast HPLC (1 day) Fast GC (1 day)

• •

Runcorn - Thermo Scientific Central Scotland - Strathclyde and Caledonian Universities Central London - Kings College North East - Sunderland University

Maximum delegate number - 6

“Well presented and entertaining. The Tutor tailored the day well to the group who had a range of skills and was very approachable and friendly” Large Pharmaceutical Company

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Off- Si te Tr ai ni ng

Off-Site Short Courses These courses are an ideal introduction to a technique, or for those looking to quickly ‘top-up’ existing knowledge. Interactive tutorials are integral to all our courses and give the attendee the opportunity to apply new found knowledge gained throughout. All courses form part of a modular training program, so these off-site courses will fit in well with all career development programs. This extensive series of courses run at various locations throughout the UK.

Short Course Titles

Short Course Venues

• • • • • • • • • •

• • • • •

Fundamentals of HPLC (1 day) HPLC Troubleshooting & Maintenance (1 day) LC-MS/MS for the Chromatographer (1 day) LC-MS Interpretation (1 day) Fundamentals of GC (1 day) GC Troubleshooting & Maintenance (1 day) GC-MS for the chromatographer (1 day) GC-MS Interpretation (1 day) Introduction to Analytical Method Validation (1 day) Techniques in Solid Phase Extraction ( 1 day)

Reading Glasgow Runcorn Milton Keynes London

Maximum delegate number - 20

“Received extremely well by both new and experienced analysts. Attendees gained a lot and commented on the trainer’s very effective presenting style. I would not hesitate to use Crawford for future training” SME Pharmaceutical Company

Further information contact: [email protected]

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On- Si te Tr ai ni ng

On-Site Training All our courses can be run at your site. This means that we can customise the course content to meet your exact requirements. Courses can be based around existing methods and instrumentation. Course objectives can also be more tightly defined, for example good maintenance procedures can be produced or new methods developed as part of a course that will have direct work-place relevance. In this way immediate return on investment is provided. We recommended numbers are limited to 10 attendees per course (6 for a practical course). This enhances course interactivity and provides greater hands-on exposure in the case of practical courses. Course assessment is available on request. The following list shows the diverse range of companies we continue to work with to deliver one of the most respected chromatography training programs both in the UK and in Europe:

Pharmaceutical & Biopharmaceutical:

Non pharmaceutical:

GSK Pfizer Bristol Myers Squibb Astra Zeneca 3M Healthcare Genzyme Vericore NAPP Pharmaceuticals UCB Celltech Covance GE Healthcare Penn pharma Celltech

FERA Coors Brewers Scottish Whisky Research Institute British American Tobacco Alcontrol Synthomer Intertek Johnson Matthey Environment Agency Forensic Science Service Fuji Imaging The Laboratory of the Government Chemist (LGC) Domino UK Ltd

And many more . . .

And many more . . .

“This is just a brief note to say how pleased all the team have been with the training documentation prepared by our Crawford Scientific Trainer. It is extremely professional. The training sessions also were greatly appreciated and everyone found him most helpful. Just the kind of employee that you want!” University Start-Up Company

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M odul ar Tr ai ni ng

Modular Training All Crawford Scientific training events have been designed as part of a Modular Training System. Whether you have numerous years of experience or are relatively new to the technique, the chances are we have courses to suit your requirements. Further, by completing all modules within a Stream you can become a certified ‘Crawford Accredited User’. We have a tiered course structure that facilitates a logical progression through the various levels In this way we aim to provide continued development for the delegate in all core subject areas.

LEVEL 5 Advanced Technologies II

Fast HPLC Practical Based

LEVEL 4 Advanced Technologies I

Advanced HPLC Method Development Practical Based

Fast GC Practical Based

LEVEL 3 Method Development and MS Interpretation

HPLC Method Development Practical or Classroom Based

Practical GC Method Development Practical Based

GC-MS Interpretation Classroom Based

LC-MS Interpretation Classroom Based

LEVEL 2 Troubleshooting and Maintenance

HPLC T&M Practical or Classroom Based

GC T&M Practical or Classroom Based

Practical GC-MS Practical Based

Practical LC-MS Practical Based

LEVEL 1 The Fundamentals

Fundamental HPLC Classroom Based

Fundamental GC Classroom Based

GC-MS for the Chromatographer Classroom Based

LC-MS for the Chromatographer Classroom Based

HPLC

GC

GC-MS

Further information contact: [email protected]

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LC-MS

Addi ti onal Tr ai ni ng

Additional Training In addition to our standard chromatography courses, Crawford Scientific offer courses in a wide range analytical techniques and data analysis methods. Whether you want to develop your knowledge of bio-chromatography or get a more in depth understanding of Statistics, we have a variety of training courses to suit your needs. As per our chromatography courses, some of the subjects are available in a tiered course structure that facilitates a logical progression through the various levels.

LEVEL 2 Advanced Courses

LEVEL 1 Basic Courses

LEVEL 1 Basic Courses

Mass-spectrometry of Biomolecules

Advanced Statistics for Analytical Chemists

Introduction to Biomolecule analysis

Introduction to Statistics for Analytical Chemists

Fundamental ICP-MS

Introduction to Dissolution

Biomolecule

Statistics

ICP

Dissolution

Analytical method validation

Basic laboratory skills

Solid phase extraction

Introduction to basic chemistry

Validation

Lab Skills

SPE

Chemistry

We also develop and deliver bespoke courses to suit particular client needs. If you have an interest in a course not listed in this brochure but which has a direct or indirect link to chromatography, please contact us and we will do our best to fulfil your requirements.

Book online: www.crawfordscientific.com/training-online-calendar.asp

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H PLC

The Fundamentals of HPLC Classroom Based 1 Day Course: Level 1 Course Description: This one-day course introduces the fundamentally important concepts associated with HPLC analysis including hardware basics, modes of analysis, basic troubleshooting, column chemistry, principles of ionisation and more. Suitable as a refresher for the more experienced chemist or as an invaluable introduction to the technique for those with limited experience, this course provides an invaluable insight into HPLC principles and practice. • • • •

Basics of the Chromatographic Process Sample Preparation Protocols Separation Mode / Retention Mechanisms Quantitation

• • •

Injectors and Columns Detectors Measuring & Optimising Chromatographic Parameters

HPLC Troubleshooting and Maintenance Classroom Based 1 Day Course: Level 2 Course Description: A logical approach to HPLC Troubleshooting and Maintenance is fully explored within this one-day course. Commonly encountered problems and best practice are explored for all major system components including Eluent, Pump, Injection system, Column and Detector. Troubleshooting separation chemistry, interrogation of poor peak shape and retention problems are a fundamentally important part of the course, leading to increased awareness of separation chemistry. Practical exercises are fundamental to this course and familiar equipment can be used to carry out a wide range of laboratory-based tasks. • • •

• • •

Approaches to Logical Troubleshooting Component Perspective Columns

Symptomatic Perspective - Baselines Symptomatic Perspective - Peaks Common Maintenance Procedures

Practical HPLC Troubleshooting and Maintenance Classroom and Laboratory Based 2 Day Course: Level 2 Course Description: As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course. • • •

Instrument problems - identifying the issues Common Maintenance Schedule Operations Investigative Operations

• • •

Hands-on Pump, Auto sampler and Detector Maintenance procedures Common Calibration Putting it all together

Further information contact: [email protected]

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H PLC HPLC Method Development Classroom Based 2 Day Course: Level 3 Course Description: For the experienced chromatographer, this course provides a step-by-step approach to method development. The course includes all of the crucial aspects of method development including; Information gathering, Mode of Chromatography, Development of Eluent systems, Column choice, Principles of Ionisation, Optimisation of important Chromatographic Parameters (R(s), a , k’ and N) and Detector choice and optimisation. Each aspect is discussed in detail with relevant examples used to demonstrate theoretical principles and software based exercises are included to give further understanding. • • •

• • •

Setting Method Development Objectives Sample Preparation System choices

Choosing a Column & Mobile Phase Optimisation Strategies Quantitation & System Characterisation

Practical HPLC Method Development Classroom and Laboratory Based 2 Day Course: Level 3 Course Description: As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course. • •

Effect of Column Parameters Developing methods for neutral samples

• •

Developing a separation for a mixture of neutral, acidic and basic compounds Using a scouting gradient

Advanced Practical HPLC Method Development Classroom and Laboratory Based 2 Day Course: Level 4 Course Description: This course follows on directly from Level 3 and will introduce attendees to the concepts and practical use of modern HPLC / uHPLC column technology. ‘How and when’ to use certain phases will be discussed in detail, as will the advantage of separation modes such as HILIC / Mixed Mode in enhancing MS sensitivity. A thorough examination of the use of silica as the support will be undertaken and the critical parameters of the support material, and their practical impact, will also be carried out. Conversion of methods (geometrical scaling) from standard column dimensions to reduced particle size columns and SPS columns will also be examined. • •

Solvent, Column and Instrumentation Considerations Transferring & Developing Methods

• • •

HPLC RP Stationary Phases Column Orthogonality HILIC and Mix-Mode Column Technology

Book online: www.crawfordscientific.com/training-online-calendar.asp

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H PLC

Fast & Ultra Fast HPLC Classroom and Laboratory Based 1 Day Course: Level 5 Course Description: This course is designed as a comprehensive introduction to the principles and practice of Fast and Ultra-Fast HPLC. The course is a mix of theory, computer simulation and lab sessions that allow the student to quickly gain knowledge on the theory, practice and limitations of the common approaches to speeding up HPLC analysis. The main factors in speeding up analysis - reduced column dimension, sub 2mm particles, superficially porous silica all combined with elevated temperature and pressure - are discussed in detail and method conversions are attempted practically. Instrumentation is also taken into account and the various approaches to Fast HPLC using conventional pressure as well as Ultra High Pressure system hardware and the importance of extra column volume and hysteresis effects are also discussed and demonstrated. The overall aim of the training is to enable clients to speed up existing separations or to develop novel ‘Fast’ HPLC separations. • • • • • • •

Acronyms and Nomenclature Why Fast HPLC? - separation speed as a limiting factor in analysis Resolution / Selectivity / Efficiency Broad Choices for Optimising Efficiency Optimising Plate Number Effectively measuring plate number / reduced plate heights / Poppe Plots So what happened to selectivity?

• • • • • • •

Effects of high pressure on Solvent and Column Properties The influence of column geometry Importance of temperature control Temperature and flow rate interdependence Instrument requirements Simple Software tools for scaling separations Transferring & Developing Methods

“I was very pleased with the “can-do” attitude to training that Crawford displayed training courses were tailored to our specific needs and delivered on-site, incorporating our local procedures and guidelines. Training materials were of a high quality and the trainers combined up to date scientific knowledge with key teaching skills.” University Spin-Out Company

Further information contact: [email protected]

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GC The Fundamentals of GC Classroom Based 1 Day Course: Level 1 Course Description: For the less experienced chromatographer or those wishing to update their skills, this course covers the fundamentally important concepts in modern GC analysis. Basics of the chromatographic process, sample preparation, inlet systems, column and detector selection are important topics covered to give the participant a thorough grounding in the technique. Instrument hardware is also covered with basic troubleshooting and maintenance tips as well as an introduction to chromatographic optimisation. • • •

• • •

Basics of the Chromatography Process Sample Preparation Protocols Sample Introduction

Columns and Temperature programming Detectors Measuring & Optimising Chromatographic Parameters

GC Troubleshooting and Maintenance Classroom Based 1 Day Course: Level 2 Course Description: A logical approach to troubleshooting is explored using both the Component (hardware based) and Symptomatic (chromatogram based) perspectives. Best practice for instrument maintenance and column handling as well as routines for cleaning and deactivating inlet and detection systems is discussed to aid the user in prolonging the intervals between essential system maintenance. In-depth treatment of the causes of peak shape and baseline anomalies are fully covered, this course is invaluable to anyone who wishes to gain further insight into the problems associated with GC analysis. • • •

• • •

Approaches to Logical Troubleshooting Component Perspective Columns

Symptomatic Perspective - Baselines Symptomatic Perspective - Peaks Routine Maintenance and Protocols

Practical GC Troubleshooting and Maintenance Classroom and Laboratory Based 2 Day Course: Level 2

Course Description: As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course. • •

Column Installation Inlet Maintenance

• • •

Inlet and Oven Parameters Detector Maintenance Developing Troubleshooting Strategys

Book online: www.crawfordscientific.com/training-online-calendar.asp

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GC

Practical GC Method Development Classroom and Laboratory Based 2 Day Course: Level 3 Course Description: Column dimensions, phase type, inlet type and operating conditions, detector settings and optimisation along with sample preparation regimes are all crucial and often baffling choices available to the chromatographer developing methods for GC analysis. This course takes a logical and progressive approach to the subject and discusses the important ‘make or break’ choices. Supplemented by a host of real world separation examples, tutorial and practical exercises to aid understanding. More experienced chromatographers can use this course to cut method development lead times drastically, armed with a logical approach and a wealth of new knowledge on how to make the right decision during each aspect of development. • • •

Setting Method Development Objectives Sample Preparation Inlet and Flow Rate Parameters

• • •

Choosing a Column & Temperature Optimisation Strategies Putting it all together!

Practical Fast GC Classroom and Laboratory Based 1 Day Course: Level 4 Course Description: This course is designed as a comprehensive introduction to the principles and practice of Fast Gas Chromatography. The course is a mix of theory, simulation and lab sessions that allow the student to quickly gain knowledge on the theory, practice and limitations of the common approaches to speeding up GC analysis. The main factors in speeding up analysis - reduced column internal diameter, use of shorter columns, use of hydrogen as a carrier with increased carrier gas linear velocity and the use of low thermal mass and electrically heated columns - are discussed in detail and method conversions are attempted practically. Instrumentation is also taken into account and the various approaches to Fast GC using standard GC equipment as well as the instrument requirements to use very small small i.d. columns and alternatively heated columns are discussed at length. The overall aim of the training is to ‘enable’ clients to speed up existing separations or to develop novel ‘Fast’ GC separations using existing equipment or to understand how to quickly adapt equipment to take advantage of these enabling technologies. • • • •

Why Fast GC? - separation speed as a limiting factor in analysis Resolution / Selectivity / Efficiency (Chemistry and Physics!) Broad Choices for Optimising Efficiency in GC Optimising Plate Number - different theories and approaches

• • • • • •

So what happened to selectivity? Working with Hydrogen at High Flow Adapting instruments for use with 100mm i.d. GC Columns Translating existing methods for use with Fast GC Data capture and analysis requirements Developing Fast GC Methods

Further information contact: [email protected]

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LC M S LC-MS/MS for the Chromatographer Classroom Based 1 Day Course: Level 1 Course Description: The Atmospheric Pressure Interface (API) is the core element to the course with the principles of operation, limitations and applicability fully explored. The course covers ion suppression, the use of Electrospray or APCI and MS-MS data acquisition modes. Optimisation of interface and mass filter settings and how to best utilise reduced dimension LC to speed up sample throughput will be discussed. All popular interface types and mass analysing equipment (Quadrupole, TOF, Ion Trap etc.) will be comprehensively covered. • • •

• • •

HPLC Considerations Sensitivity and Throughput Ion Sources/Ion Production

Detector Systems Tuning & Calibration Quantitation

Practical LC-MS/MS Classroom and Laboratory Based 2 Day Course: Level 2 Course Description: This course is not only designed to bolster your theoretical understanding of LC-MS as a technique, but to give you the practical skills needed to gain maximum benefit from your LC-MS detector. The subject theory is practically reinforced by running a range of specifically designed experiments that highlight the capabilities and limitations of Mass Analysers including Singe Quad, Triple Quad and Ion Trap Instruments using a range of samples. Both theoretical and practical time is devoted to ensuring that interface (LC) methods are sufficiently optimised such that greatest benefit is achieved from your Mass Spectrometric Detector. • • •

• • • •

Fundamentals Review Mass Analysers Mass Accuracy and Resolution

Practical Considerations of Chromatographic Methods Scan and SIM Functions In Source CID Developing MRM Experiments

“Many thanks for the slides and tutorial materials. I have just had a quick look at both and they are excellent. We, universally, considered the course to be outstanding and really appreciated the time you took to explain all our queries.” Blue Chip Environmental Client

Book online: www.crawfordscientific.com/training-online-calendar.asp

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165

141 85

41

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40

50

60

70

80

97 98 101

90

137

123 128

155 151

157

100 110 120 130 140 150

183 158

160 170

179

[M+H]+ 225

m/z 180 190 200 210 220 230

LC-MS Spectral Interpretation Classroom Based 1 Day Course: Level 3 Do you currently use LC-MS in your laboratory applications? Would you like to benefit from a greater understanding of how to interpret the mass spectra produced by Electrospray Ionisation (ESI) and Atmospheric Pressure Chemical Ionisation (APCI)? If so then this course will be invaluable to increasing your confidence and understanding of: • • • •

Mass spectral data analysis Structural elucidation Product ion generation by Collision Induced Dissociation (CID) characterization Advantages and limitations of common mass analyser types

Interpretation of low molecular weight compound mass spectra considers the importance of: • Understanding isotope patterns and their relative signal responses in assigning elemental composition and confirming chemical structure. • Understanding how common characteristic production fragmentation series are produced through atomic electronic effects. • A mechanistic understanding of the Inductive (I) and Alpha (a) cleavage. • How CID fragmentation can be invaluable for sample identification purposes through first principles interpretation and database searching. • Inclusion of peptide sequencing by tandem mass spectrometry as required. Interpretation of high molecular weight compound mass spectra considers the importance of: • When to report monoisotopic mass or average mass. • The process of deconvolution of an ESI multiply charged ion series to derive the molecular weight of a compound. Further: • Explanation of the formation of common adduct ions under different solvent and buffer conditions. • Solution and source compound dimerisation effects. • The process of data dependent acquisition and modern detector systems. • A comprehensive set of tutor-led tutorial questions to facilitate the learning process.

Further information contact: [email protected]

15

LC M S

113

47

Abundance

60

45

9000 8000

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7000

48

31

6000

31

i

α

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CH2

33

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47

5000 4000

27

GC M S

3000 2000 1000 0

78

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43 18

20

26 25

33 34 30

35

57 40

45

50

55

61 60

80 65

70

75

80

m/z

m/z = 60

GC-MS For the Chromatographer Classroom Based 1 Day Course: Level 1 Course Description: A course designed to highlight the powerful possibilities of GC analysis with spectral detection. Tuning and tune reports will be explained and instruction given in the use of tune reports as a powerful diagnostic tool. The functionality of the MS will be discussed in detail including principles of quadrupole and ion trap mass filters. Ion detection devices will be explained and the use of electron impact and chemical ionisation sources will be investigated with applicability to practical situations. GC Parameters will also be considered with operation and troubleshooting of inlets and columns explained. • • •

• • •

Chromatographic Considerations Sample Introduction MS Hardware

Detector Systems Tuning and Calibration Quantitation

Practical GC-MS Classroom and Laboratory Based 2 Day Course: Level 2 Course Description: This course is designed to enable you to gain the maximum benefit from Gas Chromatography with a Mass Spectrometric detector. The course will introduce you to quadrupole mass analysers and you will leave with the ability to manually tune the mass spectrometer for optimum performance – either quantitative or qualitative. Various spectral experiment types are explored and ALL GC and MS settings and parameters are explained in great detail, including their effect on various application types. Feel free to bring along your samples to practice with! • • • •

• • • •

Ionisation The Vacuum System Mass Filtering Ion Detection

Instrument optimisation Understanding and Troubleshooting Tune reports GC Requirements MS Data Analysis

GC-MS Spectral Interpretation Classroom Based 1 Day Course: Level 3 Course Description: This course provides the basic knowledge required for the interpretation of unknown spectra. Starting from first principles, it introduces the fundamental chemistry of mass spectrometry and builds up to an examination of the most frequently encountered fragmentation patterns. This course is not instrument-specific and is suitable for anyone involved in mass spectrometry. • • •

Obtaining good quality Spectra Basic Chemistry Principles of Ionisation

• • •

Fragmentation Mechanisms Interpretation Rules Interpretation Practice

Book online: www.crawfordscientific.com/training-online-calendar.asp

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Other C our s es

Introduction to Analytical Validation Classroom Based 1 Day Course Course Description: This one day course covers all of the basics required to produce an analytical method development protocol, implement the validation studies and report to FDA standards. • • • • • • • • • • • •

Analytical Best Practice What is Method Validation? Who defines Method Validation Requirements? Equipment Method Development & Validation Applicability Documentation Sources of error Defining Extent & Scope of Validation Studies Experimental Design for Analytical Method Validation Statistical Methods and Tests for Validation Overview of Equipment Validation

Validation Parameters: • Selectivity • Linearity • Accuracy • Precision • Sensitivity • Robustness

Solid Phase Extraction Course Description: A comprehensive one day course designed to increase expertise and optimise method development time for all users of solid phase extraction as a means of sample preparation. Practical exercises included to re-enforce learning. A must for all who use Solid Phase Extraction as a sample preparation technique Our SPE course is backed with a host of explanatory images and movies from our range of e-learning products. • • •

Basics of SPE Practical Considerations Sorbent Selection

• • •

Extraction Techniques Method Development Protocol Troubleshooting

“The diagrams were clear and trainer explained things when needed. Thorough notes and exercises helped put theory into practice.” Large Pharmaceutical Client

Further information contact: [email protected]

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Other C our s es Dissolution Training Classroom Based 1 Day Course Course Description: This one day course is an excellent introduction to the theory and principles of Dissolution Testing. This often heavily regulated technique often appears simple to use and employ within the laboratory but the underlying theory, significance of the various apparatus and test methodologies is complex. This course effectively condenses the important theory, with critical calibration, verification and practical information which makes it a must for anyone using dissolution equipment. • • • •

USP Dissolution Dissolution Theory Performance Verification Testing The Practical

Maximum number of delegates is 6. 2 dissolution baths required for practical element in the afternoon session.

ICP/MS Classroom Based 1 Day Course Course Description: This one-day course introduces delegates to the fundamental concepts and principles associated with ICP-MS. Topics covered allow the ICP-MS user to gain a useful insight into the operating principles of the instrument This course is ideally tailored towards either the experienced user who is looking for a refresher course or towards a new user who is looking for an introduction to the analytical technique. • •

An introduction to ICP-MS The Mass Spectrometer

• • •

Optimisation Quantitation Techniques Interferences in ICP-MS

Book online: www.crawfordscientific.com/training-online-calendar.asp

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Other C our s es

Introduction to Statistics for Analytical Chemists Classroom Based 1 Day Course Course Description: This course comprehensively covers the basic concepts and methods of statistics with applications in the field of analytical sciences. During this course, we will demonstrate methods of exploring, organising, and presenting data, and introduce the fundamentals of probability. We will: • •

explore the foundations of statistical inference, including the concepts of parameters and estimates explore the use of statistical measures, confidence intervals, and hypothesis tests.

The aim of the course is to foster an interest in applied statistics and equip scientists and project managers to confidently apply statistical techniques to investigate and improve data quality. Course topics include: • • •

• • •

Quantifying Errors Normal (Gaussian) Distribution Significance Tests

Statistical Measures Confidence Limits Analysis of Variance

Advanced Statistics for Analytical Chemists Classroom Based 1 Day Course Course Description: This course comprehensively covers the advanced concepts and methods of statistics with applications in the field of analytical sciences. During this course, we will demonstrate how to use: • •

Analysis of variance Randomisation and manipulation of controllable variables to reduce variability.

In this course, we will: • •

explore the use of quality control methods, experimental designs and optimisation explore the use of multivariate analysis.

The aim of the course is to expand your knowledge of applied statistics and equip scientists and project managers to confidently apply statistical techniques to investigate and improve data quality. Course topics include: • • •

Shewhart charts Cusum charts Two-way ANOVA

• • •

Factorial design and optimisation Modelling Regressions

Further information contact: [email protected]

19

Other C our s es Introduction to Biomolecule analysis Classroom Based 1 Day Course Course Description: This course provides an overview of the biopharmaceutical manufacturing process in order to demonstrate the different analytical techniques which might be used in the research, development and manufacturing processes. The aim of the course is to highlight the mode of chromatography used and to present several examples which explain current good practice and the latest developments in each technique. In this course, we will: • • •

Understand how to analyse proteins, peptides and amino acids Discuss the different requirement for developing a biomolecule and highlight the appropriate analytical techniques Provide an overview of the analytical challenges and popular techniques for companies manufacturing proteins and peptides or making bio-similars / bio-betters

The aim of the course is to provide an up to date review of the various analytical techniques required to develop and manufacture biological entities and to explore new developments which present significant analytical benefits. We will study: • • •

Reversed phase analysis of intact Proteins Reversed Phase – Peptides Analysis Reversed Phase – Amino Acid Analysis

• • •

HILIC – Glycan Analysis Ion Exchange – Proteins Size Exclusion – Proteins

Biomolecule analysis by Mass Spectrometry Classroom Based 1 Day Course Course Description: This course deals with the specifics of using mass spectrometric techniques in the field of proteomics or protein biopharmaceuticals. The aim of the course is to provide an understanding of the field of bottom-up proteomics or protein identification following proteolysis. In this course, we will consider: • • •

Biological mass spectrometry theory and hardware Sample Preparation and Nano LC systems On-line trapping and on-column loading techniques

• •

Developing LC separations to improve protein identification Database searching & analysing results

By the end of this course, we will have foster an interest in applied mass-spectrometry for the analysis of biomolecule (e.g. Peptides, Proteins and Antibodies) and equip scientists and project managers to confidently apply these techniques to investigate and improve the analytical approach to determine some crucial bio-parameters using MS. Course topics include: • • • • • •

Types of mass analyser and data generated Trapping and on-column loading techniques Protein reduction/alkylation and proteolysis Solid Phase Extraction and Sample Clean-up Gradient conditions for peptide Methods of peptide ionization

• • • • • •

20

Optimising gradient conditions Gel-based separations Principles of protein identification Nano HPLC systems and key features Using MASCOT & Types of Search Engine Statistical scoring and False Discovery Rates

Other C our s es

Software / Hardware specific training Over the past 16 years we have supported our clients with instrument and software specific training and have developed a great deal of in-house knowledge on systems and software. We can provide training on an extensive range of GC, HPLC and MS systems and software packages. We specialise in training on: • • • • • • •

Instrument set-up, preparation and optimisation Best practice and optimising data acquisition parameters Sequence automation including the incorporation of system suitability, calibration and QC checks High through data processing including building broadly applicable integration parameters Building Calibration models and automated qualitative data processing Statistical analysis of data (including automated statistical analysis is the software package allows) Marcos and programming to achieve bespoke software operation for data acquisition and reprocessing

Each training event can be a tailored version of any of our existing courses, for example: • • •

Troubleshooting and Maintenance training for specific equipment Method development using specific systems and software MS interpretation with a specific software package

We also provide bespoke consultancy services in laboratory efficiency improvement, method development/ improvement and new equipment optimisation. Please contact us with your specific requirements and we will be happy to advise on the most effective program for you.

“This was the best course I have attended. Very relevant & focused on areas we use over others we don’t” Quality Control Department – food and beverage company

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Bas i c Sk i l l s Induction and Basic Laboratory Skills Course Classroom Based 1 Day Course Course Description: Why waste valuable time and money preparing basic laboratory skills courses and induction training for new laboratory staff? Allow Crawford Scientific training professionals to design and implement training around your existing procedures and basic laboratory instrumentation. •

• • •

Ensure that all staff are trained in basic laboratory operations such as weighing, pipetting, titrimetry, volumetric work, moisture determination, pH determination, use of laboratory notebooks etc * Several major UK companies already use our training design service to supply basic and refresher skills courses which are then delivered by in-house personnel who have been trained by CSTS professionals Our custom design service can incorporate your existing SOPs’ and Protocols so that new staff are skilled in basic laboratory operations and are all following current best practice An additional benefit is an ideal opportunity to review your current laboratory practices to ensure they are compliant with current regulatory guidelines and best practice Contact us to book a ‘free’ review session now and enjoy the benefits of a laboratory run on sound fundamental working practices

Basic Chemistry A half or one day refresher course for Analytical Scientists Course Description: For anyone who is new to analytical sciences or for anyone brave enough to admit to having forgotten some of the fundamental principles! This Basic Chemistry short course can be customised to suit the needs of particular groups of scientists and aims to cover the underpinning chemical principles of solution based and instrumental analytical science. Topics should be customised to particular learner groups - typical one day course would cover 2 - 3 units of instruction, depending upon topics chosen. All topics contain worked examples & tutorial exercises. • • • •

• • • •

Measurements and Calculations Atoms and Molecules Atomic Interaction Acids Bases and Salts

Aqueous Solutions Reactions in Solution General Organic Chemistry Data Treatment

Book online: www.crawfordscientific.com/training-online-calendar.asp

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C ons ul tanc y Ser v i c es

Consultancy Services Through experience gained in supporting our clients with their application and instrument problems, we have developed a range of services which can help you improve laboratory performance. These include; HPLC method translation to improve throughput or resolution using UHPLC or Core-Shell particles HPLC and GC Method Advice On-site – review and improve critical or difficult methods GC Method Translation to Fast GC using existing equipment and narrow bore GC columns GC Method translation from Helium to Hydrogen (we can also supply the gas generators for you) MS ionisation and mass analyser optimisation service to improve sensitivity, specificity and robustness Data reprocessing training and software development to increase automation and reduce processing time Development of smart integration algorithms to reduce manual integration Macro writing Method review and improvement service Sample preparation protocol development and optimisation Instrument optimisation to improve method robustness, sensitivity and throughput Instrument sourcing and demo concierge service Method transfer facilitation

Further information contact: [email protected]

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Crawford Scientific Ltd., Holm Street, Strathaven, Lanarkshire, ML10 6NB Tel: +44 (0)1357 522961 | Fax: +44 (0)1357 522168 | email: [email protected] www.crawfordscientific.com | www.chromacademy.com