THE ART TO CLEAR SOLUTIONS

PHARMA AND BIOTECH 3

FERMENTATION The core process in biotechnology

Membranes are often installed upstream of the cultivation tank to pre-treat the raw materials. They are used for cleaning, fractionation or concentration during the processing of the products. Furthermore, they are essential for the production of ultrapure water and closed water loops. MICRODYN-NADIR GmbH, which emerged from the membrane activities of Hoechst AG and the AKZO AG, looks back on a wealth of experience in the development, manufacturing and application of membranes and modules. Based on this expertise, we have contributed to the continuous advancement of membrane technology, especially in the biotechnological and pharmaceutical industries. Fermentation is the production of substances facilitated by yeasts, bacteria or cells. These processes are well established in the production of pharmaceuticals and in the food industry. In addition, fermentation processes have gained importance in the so-called “white” or industrial biotechnology for the production of enzymes, bulk chemicals, and, last but not least, biofuels. The isolation of the pure product from the fermentation broth presents a major challenge to modern separation technology. Membrane technology has established itself as a key technology in this field since it offers several unique advantages compared to thermal, physical or chemical separation techniques.

A D VANTAG E S » high selectivity (sharp cut-offs) » gentle procedure » low energy consumption compared to thermal processes » simple plant design » wide variety of membranes and module types 4

MICRODYN-NADIR offers a wide product portfolio. We supply spiral wound modules, hollow fiber and tubular membrane modules, as well as flat membranes and formats. Our membranes have been developed based on different polymers, such as PP, PE, PES, PS, PVDF. These membranes provide high chemical and mechanical stability, they are very easy to clean and maintain stable fluxes . Today MICRODYN-NADIR’s membranes and modules are installed at leading, globally operating biopharmaceutical companies in the production of monoclonal antibodies, human and veterinary vaccines, antibiotics, transgenic proteins, enzymes and other pharmaceutical substances.

nutrient supply

microfiltration (solids separation)

ultrafiltration (diafiltration)

fermentation

standardization

ultrafiltration (concentration)

spray dryer

PRODUCTION OF ENZYMES Process scheme

Fermentation and the subsequent treatment with membrane filtration are the core processes in the biotechnology industry. Typical fermentation products include enzymes to be used as additives for foods and washing agents, among others. To achieve high yields in the cultivation tank, the cell concentration in the fermentation broth is kept at a high level. In the process described above the product exists outside the cells. After the broth has left the cultivation tank, suspended solids (cell fragments and bacteria) can be separated from the enzyme solution using a decanter. Subsequently the product solution is treated in a microfiltration unit and then further purification is done in a dia-filtration unit with ultrafiltration (UF) membranes.

During dia-filtration, substances with low molecular weight are washed out of the solution using clean solvent (ultrapure water), while the target molecules are retained by the membrane. SPIRA-CEL® spiral wound modules are recommended because this process requires a large membrane area. The modules consist of PES membranes which are especially suited for this application because of their high flux and closely defined molecular weight cut-off (MWCO). After dia-filtration, the product concentration can be increased by factor 25. Due to the higher concentration of suspended solids, the viscosity of the suspension will increase.

INNOVATIVE MODULES for crossflow filtration

The Crossflow Principle

feed flow

The efficiency of the crossflow filtration of liquids is determined by the correct choice of membrane type and construction of the module as well as the main parameters of the process – pressure and crossflow velocity. The spiral wound module SPIRA-CEL® has the most efficient geometrical design to achieve maximum membrane surface area per volume. Within the spiral wound module, the fluid flows along a spacer net that introduces turbulence. The geometry of the net can be chosen to adjust the entire configuration to meet the needs of the specific process. MICRODYN and SEPRODYN ® capillary and tubular modules are easy to operate because of their open flow channels. The diameters of the capillaries and tubes are chosen according to the demands of the process and depending on the content of solids. The cleaning process is usually facilitated by short back pulses. MICRODYN-NADIR has extensive experience in the evaluation of the influencing factors to optimize membrane processes.

permeate

In crossflow filtration the fluid is pumped parallel to the membrane surface. This promotes turbulent mixing during the filtration process and avoids deposition of particles and formation of a fouling layer on the membrane.

3

1 2

1 Membrane 2 Cover layer 3 Suspended solids and bakteria

OUR SOLUTIONS

n tio ra

ilt af

ltr ru

m

od

ul

es

fo

m ry

lla

w

f ib

an ry

er

d

ca

pi

es in

LT R

A

D

YN

ho

llo

la bu

tu YN

D U

RO IC

M

rf fo

es ul

od m

®

YN D O

PR

od m

nd

ou lw

i ra sp

L® CE SE

AIR

SP

tf

es ul

s at rm fo d an es an br em m ®

IR D A N 1

ilt

ra

od

tio

n

ul

es

fo

rm

ic

ro

f il

t ra

tio

n

possible applications for our products

Applications

Advantages

Feed sterilization upstream of fermentation

Precise separation of bacteria by microfiltration

Separation of cells or cell fragments from fermentation broths

Continuous and gentle separation of the biomass from the product

Microfiltration in upstream processes prior to further purification

Safe standardization of feedstock

Concentration of proteins or other products by means of ultra or nanofiltration

Product concentration with minimal losses

Membrane filtration for downstream processing

Simple plant design and high level of automation

Diafiltration for feed/product processing

Gentle cleaning of products

Production of pure water

Safe depyrogenation of water

00790025/122016

MICRODYN-NADIR GmbH Kasteler Straße 45 65203 Wiesbaden / Germany Phone: + 49 611 962 6001 [email protected]

SEPARATION – OUR PASSION

MICRODYN-NADIR US, Inc. 2700 Sumner Blvd. Ste. 136 Raleigh, NC. 27616, USA Phone: + 1 919 341 5936 [email protected]

We will deliver products, information and services, which fully meet or exceed customer expectations. Our team focuses on continual improvement to achieve the highest possible level of customer satisfaction and to be recognized by our customers as the technology and quality leader.

MICRODYN-NADIR (Xiamen) Co. Ltd No. 66 Jinting North Road Xinglin Xiamen, China 361022 Phone: + 86 592 677 5500 [email protected]

For more than 50 years, MICRODYN-NADIR has developed innovative membranes and membrane modules for micro-, ultra- and nano-filtration as well as solutions to support our customers’ needs in operation, performance, efficient membrane processes and regulatory compliance.

We are not satisfied until our products have been successfully integrated into your customers’ plants and processes. That is our passion. Our quality system is designed to support these goals.

MICRODYN-NADIR Singapore Pte. Ltd. 2 Tuas Avenue 10 639126 Singapore Phone: +65 6457 7533 [email protected] MANN+HUMMEL Fluid Brasil Rua Antonio Ovídio Rodrigues, 845 Parque Industrial Jundiaí III (FAZGRAN) 13213-180 Jundiaí - SP Phone: +55 11 3378 7500 [email protected]

Locations Sales Offices and Cooperations

WE SUPPORT YOU – WORLDWIDE! » » » » » »

Global availability Intensive technical consulting Ideal choice of membranes and modules Support with engineering and plant design Laboratory and pilot tests After Sales Service

WWW.MICRODYN-NADIR.COM 1