Droplet generation for NGS library preparation and dPCR on the RainDrop™: In search of cancer biomarkers Dec 2015 Viviane Sternkopf

RainDance Technologies Complex Genetics Made SimpleTM

For Research Use Only. Not for Use in Diagnostic Procedures RainDance proprietary information. Not for Distribution.

RainDance Profile Unique Focus, Unified Research Platform

NGS and dPCR

Hereditary Cancers Cancer Pathology Clonal Evolution MRD Monitoring

Rapid Customer Adoption

180 Systems Installed

Strong IP and Technology

Proprietary Digital Droplet Technology Platform

Established Infrastructure

Sales: 15+ countries (Direct/Distributed)

Customers Running >275,000 Samples in 2015

Over 175 Patents and Applications Worldwide

QSR in 2015 Design controls

Leading Customers 2

Tissue and Liquid Biopsy

For Research Use Only. Not for Use in Diagnostic Procedures RainDance proprietary information. Not for Distribution.

120 FTE (25+ PhDs)

Cancer Research Continuum from Screening to Monitoring Tissue Biopsy

Liquid Biopsy

Diseased tissue

Opportunity • Annual biopsies in U.S.: 9 million • Indeterminate results: 15 - 25% • Precious samples: FFPE, FNA, Bone Marrow 3

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Challenges Sensitivity Cellularity Clonal evolution MRD

For Research Use Only. Not for Use in Diagnostic Procedures RainDance proprietary information. Not for Distribution.

Most Metastatic Tumors Detectable in ctDNA The Opportunity Circulating tumor DNA (ctDNA) detected in most metastatic cancers

Source: Bettegowda et al. Sci Transl Med (2014)

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The Challenge Circulating tumor DNA detected at low levels in plasma

For Research Use Only. Not for Use in Diagnostic Procedures RainDance proprietary information. Not for Distribution.

Only Unified Platform to Address Cancer Research Continuum • • • • • •

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10s to 100s genes Tissue & Liquid biopsy Low input Low VF Research Flexibile content Screening / Low cost Pathology • SNaPshot (NGS) • NGS • TLDA • Arrays, etc.

• Sanger • NGS • LR-PCR • Arrays, etc. 10s to 100s genes Data quality No Sanger confirm CNVs Low VUS Scalability

Hereditary Cancer (NGS)

Clonal Evolution (NGS)

MRD Monitoring (dPCR)

For Research Use Only. Not for Use in Diagnostic Procedures RainDance proprietary information. Not for Distribution.

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10s genes Liquid biopsy Low VF Low input Fast TAT Low cost RT-qPCR FISH Imaging ELISA

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Flow RT-qPCR ImmuneSeq ELISA