LingonMAX™

Revitalize Your Skin

LingonMAXTM, Revitalize Your Skin Introduction It is the wish of every woman to have smooth and fair skin. Women endlessly pursue their quest for attractive skin through the use of topical creams and cosmetics. Temporarily covering poor skin with cosmetics may provide us the look we desire, however, cosmetics will not replace our need for healthy skin. Fortunately there is now another choice to help us maintain healthy skin, and that is LingonMAX™, a new ingredient taken orally to promote healthy skin. LingonMAX™ is a Lingonberry Extract developed by global natural products pioneer BGG and advances the natural health industry with the most effective skincare product on the world supplementingredient stage. Extracted from select-grade lingonberries, LingonMAX™ meets the most demanding standards of today’s marketplace for product quality. Strengthened by BGG’s world-class customer support, LingonMAX™ is your premier and cost effective choice in obtaining a lingonberry extract with clinically proven results in improving skin condition in women.

LingonMAX™, extracted from the highest quality lingonberries found in nature is composed of powerful nutrient compounds. LingonMAX™ is a natural plant extract is produced from the lingonberry fruit (Vaccinium vitis-idaea L.) which is a wild fruit consumed in Northern Europe, Asia, Alaska and Canada for its excellent nutritional value. Principal dietary uses include sauce, juice, jams, as wines of liqueurs and a component of baked dessert products [1]. Lingonberries contain vitamins (A, E and C) and minerals (manganese), but is a plentiful source of antioxidants, such as proanthocyanidins, resveratrol, anthocyanins, and lignans [1]. BGG LingonMAX™ is made from the select-grade wild fruits sourced from pollution free areas in Northern Europe. LingonMAX™ delivers powerful antioxidants through its unique mix of three key components comprised of 35% proanthocyanidins, 10% resveratrol and 10% anthocyanins (Fig. 1).

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Anthocyanins

Resveratrol

Proanthocyanidin

Fig. 1. Chemical structures of anthocyanins, resveratrol and proanthocyanidin.

A key component in LingonMAX™ is resveratrol which is 10% of the composition. Phytoalexin resveratrol is primarily found in grapes and red wine, and is popular for its ability to increase the longevity of yeasts, worms and flies. Resveratrol in vitro demonstrates anti-cancer and antiinflammatory functions on mammalian cell cultures and the prevention of cardiovascular disease, cancer and Alzheimer disease undoubtedly suggested a possible positive effect on human health and lifeexpectancy. Compared to other members in the berry family such as rabbit eye blueberry, high bush blueberry, low bush blueberry, cranberry and bilberry, lingonberry contains an overwhelmingly higher amount of resveratrol (Fig. 2) [2,3].

Fig. 2. The resveratrol content in berries and grapes (ng/g dry weight). Another subject of note is that proanthocyanidins contained in lingonberry mainly consist of short-chain units (dimer and trimer) with higher bioavailability and potency than long-chain proanthocyanidins (Fig. 3) [2]. Lingonberry fruit also contains rich anthocyanidins with strong antioxidant function (Fig. 4).

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Fig. 3. The short-chain proanthocyanidins content in Finnish berries (mg/kg).

Fig. 4. The anthocyanidins content in Finnish berries (mg/kg).

LingonMAX™, advanced technology ensures the unique compositions. Due to difference in structure and solubility among resveratrol, anthocyanins and proanthocyanins, it is very difficult to extract three ingredients at the same time and combine them into a finished extract. The BGG Group, however, has developed a unique method to extract these compeonents that is protected with global patents (China: ZL200710122746.X; U.S.: US 7,939,111; Japan: 特许第 5324084 号). With BGG’s proprietary techniques, the potency of all three active ingredients is maintained in our lingonberry extract product. LingonMAX™ providing exceptional health benefits. The Polyphenols and Resveratrol contained in lingonberry show strong antioxidant activity, and lingonberry extracts are now used in many clinical applications for cancer, urinary tract infections, obesity, and skin care. Now you can enjoy the benefits of LingonMAX™ lingonberry extract, a new 3/9

generation of antioxidant protection.

LingonMAX™ is useful in a wide variety of applications Published papers and animal research models indicate that LingonMAX™ has the following potential applications: Strong antioxidant It is known that reactive oxygen species can damage biological molecules such as proteins, lipids, and DNA. While the human body has developed a number of systems to eliminate free radicals from the body, it is not 100% efficient. The development of many chronic and degenerative diseases, such as cancer [4], heart disease [5], and neuronal degeneration such as Alzheimer’s [6] and Parkinson’s disease [7], has been theorized to be caused by oxidative stress. Oxidative stress has also been implicated in the process of aging [8]. Lingonberry extract has abundant antioxidants including anthocyanin, proanthocyanidins and resveratrol, which have anti-aging function by scavenging free radicals efficiently, thereby diminishing the damage caused by free radicals and prevent wrinkles due to ultraviolet radiation [9,10]. The dosage used is LingonMAX™ is show effective in 50, 100 and 200 mg/kg body weight for low, middle and high dosage groups as demonstrated in our animal research. Our animal studies showed serum malondialdehyde (MDA) of older mice fed LingonMAX™ lingonberry extract was reduced and the activity of glutathione peroxidase (GSH-Px) was higher than that of control group. Lingonberry extract exhibited anti-aging ability via its anti-oxidant activity (Fig. 5).

Fig.5. Influence of LingonMAX™ supplementation on MDA and GSH-Px content in old mice.

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Significance in Oral Beauty Supplements In addition to extraordinary antioxidant activity, and potential anti-cancer activity and cardiovascular disease prevention activity, LingonMAX™ has found promising beauty supplement applications in cosmetics industry. Collagen protein and elastin are important for the skin structural well-being. Moderate cross-linking of collagen protein can maintain the skin well-being, while free radical oxidation leads to excessive crosslinking and skin wrinkles. In long-term ultraviolet irritation skin photo-aging mice model, the content of dermal hydroxyproline (HYP) and the activity of superoxide dismutase (SOD) of the lingonberryfeeding group are higher than that of model group. Lingonberry extract can protect dermal collagen protein and smooth the skin wrinkles via its strong anti-oxidation function [11,12,13,14]. Elastin can maintain skin elasticity. Free radicals and elastinase can lead to elastin decomposition. Anthocyanins and proanthocyanidins contained in LingonMAX™ can remove free radicals efficiently, and help the collagen protein maintain moderate cross-linking, reduce the production and activity of elastinase. As a result, LingonMAX™ can improve skin health conditions and make skin soft and sleek. Our animal study indicated that feeding skin-damaged mice LingonMAX™ increased skin superoxide dismutase (SOD) and hydroxyproline (HYP) content. Both SOD and HYP are beneficial to skin health. Clinical Beauty Trial Published papers showed that lingonberry extract can improve skin conditions. And our clinical trials gave a positive result on skin care. During the clinical trials, 44 females averaging 35 years of age were asked to ingest 150 mg of LingonMAX™ each day for eight weeks. Facial skin characteristics (spot, wrinkle, skin color and moisture) of these subjects were tested before and after the eight-week administration. After participants cleansed their faces, a T-01 Digital Facial Scanner was used to scan their faces generating an RGB and UV picture. The scanner was then utilized to analyze facial images, counting spots, wrinkles, skin color and moisture. The results were shown as percentage (Table 1). The smaller the value indicates the better of skin condition, and vice versa.

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Table 1: Skin conditions comparison of before and after LingonMAX™ administration ( x 土 s ) 4 Week Observation

Before %

4 Week Percentage

8 Week Percentage

8 Week Efficient

Efficient Spot

14.5±9.3

13.5±9.0

64.4%

13.2±8.7*

59.4%

Wrinkle

8.3±6.9

8.6±7.4

60.7%

7.4±7.1*

78.2%

Color

19.5±6.3

15.5±7.4

75.1%

11.7±5.2**

93.8%

Dryness

24.4±3.6

24.2±5.5

60.7%

21.7±4.2

43.8%

As the result above in Table 1, after the eighth week of administration, the facial spots and wrinkles significantly decreased (P < 0.05, the corresponding effective rates are 59.4% and 78.2%). The decreasing facial color index was even more significant than that of spots and wrinkles (P < 0.01, effective rate reached its peak at a percentage of 93.8%). The four indexes being measure in this trial (spot, wrinkle, color and dryness) exemplified a significant decrease after eight weeks, especially compared to the results presented at only four weeks. Pictures as below show us excellent effects of LingonMAX™ directly for skin improving. Facial skin conditions were tested before and after administration. Effects of skin-care as below (Images A to D). A

C

B

D

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Image A: skin color lightened, spots got thinner and facial dryness improved after eight weeks of ingesting LingonMAXTM. Image C: lighter skin color after eight weeks of ingesting LingonMAXTM. Image B and D: diminished wrinkles and pouches significantly.

Demonstrated in the results above, LingonMAX™ improves skin equally to all age groups that were tested in this trail. The effective rates of spot, wrinkle, color and dryness didn’t show significant differences according to age. Completed clinical trials demonstrated significant results. BGG’s LingonMAX™ lingonberry extract effectively delivers significant benefits in skincare and the special compositions product has gained patents protection (ZL00810084269.7, US8,501,246). Finally good news for women worldwide is here. Oral beauty within supplements can be developed with LingonMAX™, to protect our skin from UV radiation, pollution, and oxidative stress, and preserve health, youthful skin. Weight Loss Application LingonMAX™ also shows prominent anti-obesity function [15]. Feeding obese rats LingonMAX™ was found in our animal study to reduce the body and fat pad weight as well as body fat ratio.

Safety Shown throughout the trial, the concentration of LingonMAX™ resides in a safe range within 30 mg/L, therefore proven to be safer than arbutin, pine bark and grape seed, and acts as a safe and effective skinwhitening agent. The results and percentages show that LingonMAXTM is equally effective on all ages tested throughout this trial. The results of the health examination and questionnaire showed that LingonMAXTM possessed a high safety level on human bodies with a strong skin and beauty effect producing obvious improvements in skin spots, wrinkles and facial color.

LingonMAX™ Snapshot 

Derived from European Vaccinium vitis-idaea L.

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Extracted using advanced proprietary process with global patent protection.

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Highly stable: 36 month shelf life.

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Low Dosage: 150 mg/day for adults.

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Natural form of resveratrol, proanthocyanidins and anthocyanins. 7/9

14. Kenjirou O., Kazuhiro T., Junji T., et al. The protective effects of bilberry and lingonberry extracts against UV light-induced retinal photoreceptor cell damage in vitro. J. Agric. Food Chem., 2013, 61, 10345 – 10353. 15. Carine M., Michele L., Christine J., et al. Food grade lingonberry extract: polyphenolic composition and in vivo protective effect against oxidative stress. J. Agric. Food Chem., 2011, 59, 3330–3339.

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References 1.

Stang, E. J., Weis, G. G., and Klueh, J. Lingonberry: Potential new fruit for the northern United States. p. 321-323. In: J. Janick and J.E. Simon (eds.), Advances in new crops, 1990. Timber Press, Portland, OR.

2.

Maatta-Riihinen, K. R., Kahkonen, M. P., Torronen, A. R. Catechins and procyanidins in berries of vaccinium species and their antioxidant activity. J. Agric. Food Chem., 2002, 53, 8485.

3.

Heinonen, M. Antioxidant activity and antimicrobial effect of berry phenolics – a Finnish perspective. Mol. Nutr. Food Res., 2007, 51, 684.

4.

Awika, J. M., Rooney, L. W., Wu, et al. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agric. Food Chem., 2003, 51, 6657-6662.

5.

Miller, K. B., Stuart, D. A., Smith, N. L., et al. Antioxidant activity and polyphenol and procyanidin contents of selected commercially available cocoa-containing and chocolate products in the United States. J. Agric. Food Chem., 2006, 54, 4062-4068.

6.

Gu, L., House, S. E., Wu. X., et al. Procyanidin and catechin contents and antioxidant capacity of cocoa and chocolate products. J. Agric. Food Chem., 2006, 54, 4057-4061.

7.

Pandjaitan, N., Howard, L. R., Morelock, T., et al. Antioxidant capacity and phenolic content of spinach as affected by genetics and maturation. J. Agric. Food Chem., 2005, 53, 8618-8623.

8.

Bacchiocca, M., Biagiotti, E, and Ninfali, P. Nutritional and technological reasons for evaluating the antioxidant capacity of vegetable products. Ital. J. Food Sci., 2006, 18, 209-217.

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Wang, S. Y., Feng, R. T., Linda, B., et al. Antioxidant activity in lingonberry (Vaccinium VitisIdaea L.) inhibits activator protein 1, nuclear factor-kappab, and mitogen-activated protein kinases. J. Agric. Food Chem., 2005, 53, 3156.

10. Xu Shen-hong, Hang Hu. Effect of solvent and PH on 1,1 -diphenyl-2-picryl-hydrazy analytical method. Analysis Test Journal, 2000, 19, 50-52. 11. Valenzano, D. R. Resveratrol and the pharmacology of aging: a new vertebrate model to validate an old molecule. Cell Cycle, 2006, 5, 1027. 12. Baur ，J. A. Resveratrol improves health and survival of mice on a high-calorie diet. Nature, 2006, 444, 337. 13. Lagouge, M. Reveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1 α . Cell, 2006, 127, 1109. 8/9