Food Testing Solutions Chemistry & Consumables

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[ working to keep food safe ] Consumers worldwide are becoming more aware of the quality and origin of the food they consume, and are demanding stricter regulations to protect their safety. Suppliers, wholesalers, and manufacturers have voluntarily increased product inspections to comply with regulatory and public pressure. We understand the demand this places on workflow and laboratory efficiency. Waters Corporation works with governments, research organizations, and industry partners to provide accurate, fast, and cost-effective solutions to help ensure a safe food supply.

MULTI-RESIDUE SCREENING

[ multi-residue screening ] DisQuE Dis p ersiv e Sam p l e Pre pa rat ion

T he number of pesticides and metabolites being monitored in food commodities worldwide is well over 1,000 targeted analytes. In response, laboratories are being asked to broaden their range of pesticide analysis, while management is demanding cost reductions. A new technique, “QuEChERS”, standing for Quick, Easy, Cheap, Effective, Rugged, and Safe, is readily accepted by both the Association of Analytical Communities (AOAC) International and the Committee of European Normalization (CEN) for the analysis of pesticide residues in food and agricultural products. QuEChERS is a simple and straightforward sample preparation technique for multi-residue screening. For a pesticide residue laboratory, multi-residue methods increase sample throughput, decrease sample turnaround time, and optimize analyst efficiency.

Solutions and kitted methods add value to your laboratory function by addressing the need for simple, easy-to-follow protocols that require very little training. DisQuE™ dispersive sample preparation is a wellproven, high-throughput sample preparation method for the analysis of a wide array of pesticides in fruit and vegetable samples* that can be used in any testing laboratory. T he benefits of Waters DisQuE dispersive sample preparation include:  Efficient, universal sample extraction method for a wide variety of complex matrices  Validated detection levels down to 10 ng/g (ppb)*  Convenient, ready-to-use sample preparation kit improves laboratory efficiency and workflow  Quality manufacturing guarantees a reliably clean, consistent product

*Reference: Lehotay, J.AOAC Int. 90(2) 2007, 485-520.

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MULTI-RESIDUE SCREENING

402 Pesticide Residues in One 10 Minute Run at 10 ng/g (ppb) LC/MS Conditions: LC System:

Waters ACQUITY UPLC® System

MS System:

Waters ACQUITY® TQ Detector

Column:

ACQUITY UPLC BEH C18, 2.1 x 100 mm, 1.7 μm

Part Number:

186002352

Mobile Phase A:

98:2 water:methanol + 0.1% formic acid

Mobile Phase B:

Methanol + 0.1% formic acid

Injection Volume:

20 µL, full loop injection

Relative Intensity (%)

100

0 0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.5

4.0

5.0

5.5

6.0

6.5

7.0

7.5

8.0 min

Increase your laboratory's efficiency by broadening the range of pesticides determined in a single analytical run.

Recovery Data for Three Types of Sample Matrices Using the DisQuE Extraction Procedure (% Recovery)

Baby Food

Mango

Avocado

Atrazine

95

108

107

Carbendazim

74

90

111

Cyprodinil

77

90

93

Flufanacet

77

101

102

Imazalil

76

94

89

Tebuconazole

79

95

82

Thiabendazole

101

103

106

T he DisQuE Dispersive Sample Preparation Kit conforms to the AOAC official method for determining pesticide residues in fruits and vegetables.

DisQuE dispersive sample preparation provides robust universal sample extraction for a wide variety of complex matrices.

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targeted-RESIDUE analysis

[ Targeted-residue Analysis ] Public expectations for food quality have risen and our food supply is no longer taken for granted. As regulatory requirements increase, the focus is placed on rigorous testing and it becomes the laboratory's responsibility to meet the high demand for safe food.

T he benefits of a compound-specific assay include:  Reduced matrix effects and ion-suppression for LC/MS applications  Increased sensitivity by selectively removing unwanted matrix interferences

For many regulated food testing methods, the suitability for a particular assay is determined by the analyte selectivity. For the most challenging issues, the presence of interfering matrix elements can mask the analysis of the component of interest. Having confidence in the results is why many laboratories rely on analyte-specific methods. Targeted sample preparation techniques remove excess contaminants to yield clean, informative chromatograms.

 Improved instrument performance by maximizing system up-time  Enriched sample extracts for targeted compounds  Rugged and reproducible methods suitable for automation

Certified Sep-Pak®, Oasis®, and Sep-Pak SPE cartridges have unmatched performance and offer the most diverse sorbent choice and format to handle any food testing challenge.

C8

AC2

Carbon Black/PSA Carbon Black/ Aminopropyl

C18

Alumina A Alumina B Alumina N

PSA Aminopropyl

Alumina A

Certified Sep-Pak Sorbents

Florisil

Silica Silica

Alumina B

Accell Plus CM

ha

se

Alumina N

Accell™ Plus QMA ge han Ion Exc Mi xe

yP ialt Spec

Cyanopropyl

Sep-Pak Sorbents

e

ase

Florisil™

ha s

ase

tC2

dP

h al P rm

C18

se er

No

tC18

h al P rm

Diol

e

No

PS2

d P ha s

Re v

erse Rev

Mo d-

d

n E xc atio C e

hange

MCX

WCX

a se

MAX

HLB WAX

xe Mi

d

-M od e

rsed P h Reve

Oasis Sorbents

Ani on

Exc han ge

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Certified Sep-Pak SPE devices are manufactured using strict quality controls to ensure that performance and cleanliness are maintained to achieve the lowest levels of detection.

TARGETED-RESIDUE ANALYSIS Melamine Analysis Melamine and its Related Compounds 100 %

Ammeline

0

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

100

2.25

2.50

2.75

3.00

3.25

3.50

3.75

4.00

2.25

2.50

2.75

3.00

3.25

3.50

3.75

4.00

%

Melamine

0

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

Ammelide

%

100

0

0.25

0.50

0.75

1.00

100

1.25

1.50

1.75

2.00

2.25

2.50

2.75

3.00

3.25

3.50

3.75

4.00

1.75

2.00

2.25

2.50

2.75

3.00

3.25

3.50

3.75

4.00 min

Cyanuric Acid

%

Worldwide, government agencies have implemented guidelines for screening melamine and melamine-related compounds in foods, including infant formula and dairy products. T he maximum daily adult intake recommended by the World Health Organization (WHO) is set at 0.2 mg/kg (ppm) of body weight. Government agencies are in agreement and have set limits that reflect these guidelines. Melamine-tainted infant formula is of most concern because this can be the only source of nutrition for some infants. Many agencies have established zero tolerance policies to prevent adulteration of infant formulations. Working with government researchers around the globe, Waters strives to stay at the forefront of these important issues to provide the most effective solutions. By using Oasis and Sep-Pak SPE cartridges for sample preparation and the ACQUITY UPLC system, researchers have developed methods that exceed WHO expectations, reaching 30 µg/kg (ppb).

0

0.25

0.50

0.75

N

H 2N N

NH2 N

NH2

Melamine

Unparalleled chromatographic performance integrates leading technology with analytical columns, chromatographic instrumentation, and analyte detection. The ACQUITY TQD system provides reliable performance in a small, easy-to-use system solution for targeted-pesticide analysis.

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1.00

1.25

1.50

LC System:

Waters ACQUITY UPLC system

Column:

ACQUITY UPLC BEH HILIC 2.1 x 100 mm, 1.7 μm

Part Number:

186003461

Injection Volume:

10 μL

Mobile Phase A:

10 mM Ammonium acetate

Mobile Phase B:

10 mM Ammonium acetate in acetonitrile

complete Solutions

[ Complete solutions ] Developing reliable and robust methods is time consuming. Managing methods development while maintaining your routine analysis programs is often a balancing act that drains your analytical resources. Waters understands these laboratory demands and is committed to providing integrated solutions for food analysis. A comprehensive product portfolio allows your analytical teams to maximize synergy between our instrumentation, chemistry consumables, and software to provide complete solutions for a wide variety of sample analysis. Unmatched component reproducibility assures that the method solution achieved in your laboratory today will be the same year-after-year. Waters integrated solutions, backed by our worldwide technical support team, maximize productivity and virtually eliminate method development. From initial sample preparation to final data reporting, Waters simplifies and optimizes each and every step along the way.

Filt ers

Wat ers C ert ified V ials

Filtration provides immediate protection for analytical system components and minimizes downtime. In partnership with Pall Life Sciences, Waters offers filtration products that are Certified for Compliance, which means they have been designed and developed to comply with regulatory and quality objectives.

Sample vials are a critical part to sample preparation. Ensure that the vials you use do not introduce unwanted contaminants and interferences. Waters provides a wide selection of certified vials, tested to maximize sensitivity and improve detection limits for LC/UV/MS and LC/MS analysis. Do not compromise your test results; avoid ghost peaks, dislodged septa, and damaged needles.

GUA RD COLUMNS

Training and Resou rces

VanGuard™ pre-columns, Sentry™ guard columns, and Guard-Pak™ inserts prolong column lifetime by removing contaminants from the sample, giving you enhanced reproducibility and performance. T hey are packed with the same high performance stationary phases used in Waters analytical columns.

Waters conducts training courses at our regional facilities to equip scientists with essential skills and knowledge. If your team needs training in a specific area, we offer onsite education, at your laboratory, and will work with you to develop a program that fits your educational needs. www.waters.com/training

Waters also offers educational primers that are available in print or on our website. www.waters.com/primers

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complete Solutions CARBAMATE ANALYSIS

Ca rbamat e Analysis Kit

T he Carbamate Analysis Solution helps to successfully analyze samples according to government regulations, saving time and money. T he components include:

Waters Carbamate Analysis Kit includes the Waters Carbamate column, sample preparation cartridges, vials, and carbamate reference standards.

 Alliance ® HPLC system  Carbamate Analysis column  Post-column reaction module  Fluorescence detector  Empower™ Chromatography Data Software Experience the speed, superior results, and simplicity offered by the Waters Carbamate Analysis Solution. It provides a guaranteed analysis of the carbamate pesticides, in real world samples, that exceeds the sensitivity required by the AOAC.

Sof t Drink Analysis

LC Analysis of Carbamates in Apple Cider 8

Waters Soft Drink Analysis Kit offers a complete solution for determining caffeine, aspartame, benzoic acid, and sorbic acid in soft drinks in under 8 minutes. T he kit includes mobile phase, standards, and an analytical column, all optimized for soft drink analysis.

10 4 123

5 6

9

7

11

0

Conditions: LC System: Column: Part Number: Mobile Phase: Detection: Injection Volume: Sample:

12

15

Alliance for Carbamate Analysis Waters Carbamate Analysis column, 3.9 x 150 mm WAT035577 A: Water B: Methanol C: Acetontirile Ternary non-linear gradient Post-column derivitation Fluorescence at 339 nm, 445 nm 75 µL 40 mL of cider spiked @ 25 μg/L

30 min



Compounds: 1. Aldicarb Sulfoxide 2. Aldicarb Sulfane 3. Oxamyl 4. Methomyl 5. 3-Hydroxycarbodunan 6. Aldicarb 7. Propoxur 8. Carboduran 9. Carbaryl 10. 1-Nophihal 11. Methiocarb 12. BCMC

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complete Solutions Amino Acid Analysis Amino acid composition is a critical component of the nutritional value of foods and feeds. Qualitative and quantitative amino acid analysis is used to determine the concentration and identity of a protein, or to confirm the origin of natural products based on the free amino acid content of a particular commodity. W hen used for food safety testing, amino acid analysis can determine protein deficiencies in processed food and to detect food adulteration that masks true protein content. T he UPLC® amino acid analysis solution consists of:  Waters ACQUITY UPLC system and tunable UV detector  AccQ•Tag™ Ultra derivatization chemistry including reagents, and column, eluents  Empower 2 software's pre-configured projects, methods, and report formats  Full system and application level support documentation  Application-specific performance qualification UPLC Amino Acid Analysis Solution

 Connections INSIGHT® remote, intelligent services

Example of Animal Feed Hydrolysates, 6 ng on Column, Using the

Leu Ile

Phe

Val

Lys

Deriv Peak

Cys Tyr Met

Pro

Ala

Ser Arg Gly

His

Glu

Soybean Meal

Thr

Whole Soybean

.010

Asp

AMQ

Swine Diet

NH 3

AMQ

Poultry Diet

AMQ

.020

AMQ

UPLC Amino Acid Analysis Solution.

Leu Ile Phe

Val

Lys

Cys Tyr Met

Pro

Thr

Asp

Ala

Glu His

.010

Ser Arg Gly

NH 3

.020

Deriv Peak

0

Ile Leu Phe

Val

Cys Tyr Met

Pro

Lys

Deriv Peak

Glu Thr

His

.010

Ala

NH 3

.020

Asp

0

Ser Arg Gly

AU

0

1.5

2.0

2.5

Conditions: LC System: Column: Part Number: Mobile Phase A: Mobile Phase B: Gradient: Detection:

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3.0

3.5

4.0

4.5

5.0

Waters ACQUITY UPLC system AccQ•Tag Ultra, 2.1 x 100 mm, 1.7 µm 186003837 1:20 Dilution of AccQ•Tag Ultra Eluent A AccQ•Tag Ultra Eluent B AccQ•Tag Ultra Hydrolysate Method UV (TUV), 260 nm

5.5

Val

Ile Leu Phe

Lys

Deriv Peak

Cys Tyr Met

Pro

Ala

Glu

Ser Arg Gly His

.010

Thr

.020

Asp

0

NH 3

T he AccQ•Tag Ultra Amino Acid Analysis Solution combines Waters experience in derivatization chemistry with the award winning ACQUIT Y UPLC separation technology. Users feel confident with assured performance in the areas of protein characterization and nutritional analysis of food and feeds. Our solution provides performance qualified protocols that are designed to be rugged and reliable, assuring reproducible results day-to-day, instrument-to-instrument, and lab-to-lab, around the world with the expert support that scientists have come to expect from Waters.

6.0

6.5

7.0

7.5

8.0 min

Complete Solutions F ermentat ion Analysis, Organic Acids, Alcohols, and Ca rbohydrat es

Set t ing t he Standa rd in Liquid Ch romatogra phy Columns

T he analysis of alcohols and organic acids is important as these compounds typically play a role in determining the flavor characteristics of beverages, such as beer, wine, and some spirits. Furthermore, the presence of alcohols in fruit juices can indicate product deterioration. Waters offers a complete line of columns and stationary phases to determine fermentation analysis, organic acids, alcohols, and carbohydrates. T he Fast Fruit Juice column is specifically designed to effectively separate glycerol, acetic acid, and ethanol in grape and other fruit juices.

T he diverse functionalities available within Waters HPLC and UPLC column families give you the tools that answer the most complex analytical challenges.

BEH Technology™ Particle  Seamless method transfer between HPLC and UPLC methods  Excellent reproducibility, performance, and column lifetime though pH range of 1-12

Triglycerides and C hol est erol Analysis The analysis of high levels of cholesterol in samples, such as butter or antioxidants in oil, can be effectively performed, without saponification, by size exclusion using Waters Ultrasytragel® columns. Cholesterol from Butter Conditions: Column: Mobile Phase: Sample: Injection Volume: Detection:

Ultrastyragel 100Å and 500Å each column 7.8 x 300 mm CHCl3 A: Cholesterol standard, 0.125% B: Butter (1/10 w/v) = 0.24% in undiluted sample 50 µL Refractive index

A

SunFire™ Columns

B

 T he standard for chromatographic resolution, sensitivity, and peak capacity 22 min

 High mass loading capacity  Reproducible performance

FATTY ACID ANALYSIS T he Free Fatty Acid HP column uses a phenyl-bonded packing and a simple isocratic elution method to separate free fatty acids based on carbon chain length and degree of saturation. Fatty Acid Analysis Conditions: Column: Mobile Phase: Detection:

Atlantis® Columns

Free Fatty Acid HP CH3CN: THF: H20 45:20:35 Refractive index

Sample: 1. Capric Acid 2. Lauric Acid 3. Myristic Acid 4. Palmitic Acid 5. Stearic Acid 6. Nonadecanoic Acid 7. Arachidic Acid 8. Heneicosanoic Acid 9. Behenic Acid

 Designed for retention of both polar and non-polar analytes in your sample 1

2

 Reversed-phase performance in 100% aqueous mobile phases

3 4 5

6

7

8

9

5 min

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 HILIC retention for highly polar analytes

MANUFACTURING QUALITY AND DISTRIBUTION Manufac t u ring Qualit y

ERA's C ert ified Reference Mat erials

Regulations are becoming more rigorous and laboratory demands are increasing. T he methods used and created in testing facilities need to be robust and reproducible over the lifetime of the analysis.

T he word that best describes ERA’s Certified Reference Material standards is “true”. We prepare every standard with starting materials traced to NIST or the highest possible metrological authority, and we verify the preparation, production, and packaging via exhaustive in-house analyses. We analyze and test every lot for accuracy, precision, and stability. We scrutinize analytical data to make certain there are no unexpected problems with the standards, and then, only after all of that, we make them available to you. W hen you receive Certified Reference Material from ERA, you are looking for the truth. We guarantee that is what you will find.

All Waters manufacturing facilities are registered with the FDA and are ISO 9001:2000 certified. In 2006, as a continuous improvement towards quality, Waters manufacturing, research and development, and product development became ISO 13485:2003 compliant. T his assures that the entire process is strictly monitored and controlled over the lifetime of production. Unlike many suppliers, Waters controls all aspects of the production process. From base particle synthesis using raw materials, to ligand bonding and quality inspection of the final product, Waters manufacturing is accountable for every step of the process.

www.eraqc.com

Global Dist ribut ion

VICAM's Mycotoxin Test ing Solut ions

Waters direct sales and distribution network combines 97 offices serving 54 countries ensuring high quality service anywhere in the world. As a partner working with our highly trained specialists, you are guaranteed to receive the right product, on time for your specific food testing challenge.

Since 1985, VICAM has been dedicated to developing USDA- and AOAC-approved products that offer qualitative and quantitative screening for a wide variety of mycotoxins. Around the world, VICAM products are used with confidence by customers who value unmatched reliability, service and support. We guarantee satisfaction with expert tec hnical assistance and top-of-the-line customer care through a global sales and scientific distribution network. www.vicam.com

Analytical Instrumentation Certified Reference Standards

Chromatographic Consumables Informatics & Data Management

Waters understands that your work flow is jeopardized if laboratory consumables are unavailable or not delivered on time. As a global supplier, we have distribution centers in Europe, Asia, and North America.

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SOLUTIONS YOU CAN RELY ON Residue Det ec t ion  Pesticides in fruits, vegetables, and juices  Veterinary drugs in meat and fish  Chemical residues in animal feeds

Contaminant s and adult erat ion  Mycotoxin and pathogen testing  Chemical adulteration

Addit iv e monitoring (QA /QC) of food and feed  Vitamins  Amino acids  Sugars  Food supplements

Nat u ral p roduc t s  Authenticity, origin, and adulteration  Nutritional labeling  Functional foods

Ot her Resou rces  Proficiency testing  Analyst competency testing (included with application solution)  Standard procurement

The optimum solution requires focused application knowledge and effective technical support. Waters dedicated team of research scientists continuously evaluates products for a wide array of food testing challenges. W hether your challenge is sample preparation, analytical separation, or instrument optimization, we can recommend methods and advice to help you be successful. Contact your local VICAM, ERA, or Waters representative today to learn more.

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T he quality management system of Waters’ manufacturing facilities in Taunton, Massac husetts and Wexford, Ireland complies with the International Standard ISO 9001:2000 Quality Management and Quality Assurance Standards. Waters’ quality management system is periodically audited by the registering body to ensure compliance.

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© 2009 Waters Corporation. Waters, T he Science of W hat’s Possible, DisQuE, ACQUITY UPLC, ACQUITY, UPLC, Oasis, Sep-Pak, SunFire, Atlantis, BEH Technology, XBridge, MassLynx, Alliance, Empower, Connections INSIGHT, AccQ•Tag, UltraStyragel, Guard-Pak, ERA, VICAM and Sentry are trademarks of Waters Corporation. 720002544EN

April 2009

KK-FP