BELUGA IMPORT PROJECT

CONTENTS GEORGIA AQUARIUM – BELUGA IMPORT PROJECT

FACING EXTINCTION

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In less than 40 years, beluga whales will be extinct in North American aquariums – here’s what we can do

SEARCHING THE GLOBE

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In order to sustain the North American population, we began looking for options worldwide

POPULATION STUDY

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A five-year population study was commissioned to ensure that this project would have no negative impact

UTRISH MARINE STATION

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After reviewing many inadequate Russian facilities, an appropriate temporary facility was found

THE PERMIT PROCESS

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Georgia Aquarium then applied for the permit – and what should have been months turned into years

DIFFICULT DECISIONS

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After the permit denial, we were tasked with a series of decisions for the future of beluga whales

UNCERTAIN FUTURE

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Beluga whales in North America now face a future with no clear outcome

CONSERVATION

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See how we have committed to this unique species – and always will

EXPERT BIOS

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Biographies of our leading beluga whale and zoological experts

SCIENTIFIC ANALYSIS ON MARINE MAMMAL RELEASE

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Analysis from our Chief Veterinary Officer on the realities of whale and dolphin release

RESPONSES TO ROAST BEEF PRODUCTIONS

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Read the entire correspondence between Georgia Aquarium and the filmmakers

A flash drive has been provided for you. On this drive you will find:    

Photos and video taken by Georgia Aquarium zoological team while at Utrish Marine Station Photos and video of Georgia Aquarium animal care teams and beluga whales A digital version of this media kit A link to belugaimportproject.org – a website completely dedicated to this project

About belugaimportproject.org This site is dedicated solely to detailing the complexities of our beluga import project in a format that’s easy to understand. Here you will find videos, photos, and information on Georgia Aquarium and its 12year commitment to this project and beluga whales.

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GEORGIA AQUARIUM BELUGA IMPORT PROJECT – OVERVIEW The population of beluga whales in accredited North American zoological facilities is dwindling. Today, more than 23 million guests and more than one million students have been inspired by beluga whales at Georgia Aquarium in its ten years. Georgia Aquarium’s commitment to this majestic species has been ongoing prior to its opening in 2005. When faced with the reality that millions of people may never have the opportunity to witness these unique Arctic mammals, ever, in North America – Georgia Aquarium acted. Georgia Aquarium’s mission for the beluga import project was to ensure a sustainable population of beluga whales in accredited North American facilities. Maintaining a healthy population of belugas in human care is essential to this goal and serves to help the conservation of beluga whales worldwide. This required adding new whales to the current population in human care to increase the number and the genetic diversity. Throughout this guide you will have the opportunity to follow along the entire 12-year process. From studying the remote region of Far East Russia, applying for the import permit, leading into the years the permit decision-making languished in court. You will land at the permit denial and the difficult decisions that followed. Throughout the entire process we believed we acted morally, ethically, and acted in the best interest of these whales. In the past few weeks we have been made aware of a crowd-funded film that casts severe allegations at Georgia Aquarium and its beluga import project. While we have not been able to view the film, we believe the film to have a host of inaccuracies. We will continue to address these allegations head-on. Although these beluga whales will never come to the U.S. to receive the highest standard of care anywhere in the world, Georgia Aquarium remains committed to this species. As we look into the uncertain future of beluga whales not only in North America, but across the globe, Georgia Aquarium continues to protect beluga populations through research, education, and inspiring millions of people to care for them. Georgia Aquarium, its partners, and its beluga whale experts committed over 12 years to this project to sustain a population of beluga whales that needs our help. This guide will detail the complexities of the project that is so important to us – this is our story.

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Beluga Import Project Timeline 

2004: Georgia Aquarium searches zoological facilities worldwide for beluga whales to acquire. Search concludes no whales available with one exception. Two male whales, originally sourced in Russia, at a facility under a roller coaster in Mexico City, were in guarded condition and in need of rescue. Additionally, a zoological expert from Georgia Aquarium was sent to eastern Russia, Chkalov Island, to explore potential for acquisition of whales from a company that sources whales from the Sea of Okhotsk. Results of the trip indicated collection from this population could be possible but a population abundance study would be necessary to ensure compliance with Marine Mammal Protection Act (MMPA).

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2005: Georgia Aquarium rescues two beluga whales from Mexico City and acquires three female whales on breeding loan from a partner facility. Evaluation of the existing collection at Georgia Aquarium and at accredited partner facilities in North America indicates the population of whales will not sustain due to low number of animals and lack of genetic diversity.

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2007: Georgia Aquarium provides funding along with SeaWorld, Ocean Park Corp. of Hong Kong; Mystic Aquarium in Mystic, Conn.; and Kamogawa Sea World of Japan for scientists from the Russian Academy of Sciences A.N. Severtsov Institute of Ecology and Evolution to conduct a comprehensive population abundance and genetics study of the population of beluga whales in the Western Sea of Okhotsk (in the Sakhalin-Amur region of far East Russia). A second Georgia Aquarium zoological expert was sent to the far east of Russia, Chkalov Island to observe collection and release of whales for the beluga population abundance and satellite tagging study. The official confirms the process and techniques witnessed were similar to the methods used by National Oceanic and Atmospheric Administration (NOAA)/Fisheries and other researchers and agencies during cetacean tagging and health assessments; methods that are common in wildlife biology and determined to be humane (i.e. required Institutional Animal Care and Use review of protocols as regulated by the U.S. Animal Welfare Act).

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2008: Georgia Aquarium continues funding along with SeaWorld, Ocean Park Corp. of Hong Kong; Mystic Aquarium in Mystic, Conn.; and Kamogawa Sea World of Japan for an independent population abundance and genetics study of beluga whales in the Western Sea of Okhotsk.

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2009: Georgia Aquarium begins dialogue with NOAA Fisheries permits division regarding the draft of an import permit to acquire beluga whales from Russia. NOAA encourages Georgia Aquarium to apply to import an appropriate number of whales to achieve a sustainable population in human care in North America. This approach would mitigate the need to import beluga whales in the future.

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2010: A third Georgia Aquarium zoological expert was sent to Chkalov Island in the Russian far east, to observe beluga whale collection and release of whales for beluga tagging and population abundance study. The official also confirms the process and techniques witnessed were similar to the methods used by NOAA Fisheries and other researchers and agencies during cetacean tagging and health assessments; methods that are common in wildlife biology and determined to be humane. The Russian Academy of Sciences A.N. Severtsov Institute of Ecology and Evolution concludes five years of population abundance research on the wild population of beluga whales in the Western Sea of Okhotsk. The research concludes that this population of belugas is stable and that acquiring a limited number from this population will have no negative impact on its sustainability. Georgia Aquarium informs Russians of intent to import 18 whales so animals can be identified for Georgia Aquarium’s import permit application. Georgia Aquarium establishes partnership with a zoological park in Asia, Ocean Park Hong Kong, and develops plan to move whales identified for import permit to contemporary facilities at that park while waiting for permit approval.

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2011: The highly respected, International Union for the Conservation of Nature (IUCN), formed an independent scientific review panel, led by the Chair of the IUCN Species Specialists Group for Cetaceans, to assess the sustainability of live capture removals of beluga whales from Sakhalin-Amur region of the Sea of Okhotsk. The assessment was peer-reviewed and validated the removal of the whales would have no detrimental impact on this beluga whale population. IUCN’s assessment is later published and made available to the public. Georgia Aquarium and others are all listed as participants in the study. Georgia Aquarium prepares to move the whales identified for import to a contemporary facility at Ocean Park Hong Kong. Days before transport, Ocean Park Hong Kong backs out of the project due to pressure from the animal extremist community. With this change in plans, Georgia Aquarium invests in infrastructure at Utrish Marine Station to establish appropriate animal management options for the Russian team to care for the whales during the permit application review process. This investment included building larger pools, food, and animal care staff, veterinary medicine and supplies and our zoological experts and veterinarians visiting the station. Additionally, Georgia Aquarium executes contract agreement for the Russians to provide care for the whales while they are managed at the Utrish Marine Station and establishes routine schedule of Georgia Aquarium officials to visit Utrish Marine Station to monitor wellbeing of animals and provide feedback to Russian team.

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2012: Georgia Aquarium filed a permit application with NOAA Fisheries to import to the United States 18 beluga whales that had previously been collected from the Sea of Okhotsk in 2006, 2010 and 2011.

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Georgia Aquarium takes part in a hearing that allowed public comment on the import permit application. More than 15 scientific, academic, conservation, and animal welfare experts speak to support Georgia Aquarium’s permit request. 

2013: Despite already completing its extensive permit application review process, issuing an environmental assessment report and drafting the approval letter for the permit, NOAA Fisheries suddenly changed direction and denied the Aquarium’s permit application. Georgia Aquarium reviews the denial letter and disagrees with NOAA’s reason for denying the permit and files a complaint in a U.S District Court in Georgia against NOAA Fisheries and the Department of Commerce seeking to overturn the permit denial. Taking the decision to court was the only option for the Aquarium.

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Summer 2013: The first of four unfortunate losses occurs. Cause of death is not fully understood because necropsies were not conducted and Georgia Aquarium staff was not present.

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August 2014: Georgia Aquarium files a motion to supplement the record, asking the court to require the release of more than 20 documents deliberately withheld by NOAA Fisheries from consideration by the court. These records pertain to NOAA Fisheries’ early indications to approve the import, the preparation of the import and a complete environmental assessment showing that issuance of the permit complied with the law. In addition, the records pertain to any meetings and discussions that then led to the sudden reversal and denial of the permit, and NOAA’s research papers that showed their population counting methods were arbitrary, and have never been used before or since.

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November 2014: The second of four unfortunate losses occurs. Cause of death is not fully understood because necropsies were not conducted and Georgia Aquarium staff was not present.

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December 2014: The motion to supplement the record is denied. Georgia Aquarium files a motion with the court arguing that -- based on the facts before NOAA -- their permit denial was arbitrary, capricious and not in accordance with law. Georgia Aquarium asks the judge to invalidate the permit denial and to order that the permit to import the whales be issued.

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Summer 2015: The final two of four unfortunate losses occurs. Cause of death is not fully understood because necropsies were not conducted and Georgia Aquarium staff was not present.

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August 14, 2015: Georgia Aquarium participates in oral arguments to ask the court to uphold the MMPA and its strong support of zoological institutions, and order issuance of a permit to bring the belugas into the U.S. as soon as possible.

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September 2015: SeaWorld backs out of beluga import project publically and will no longer take any of the beluga whales destined for U.S. accredited facilities upon pending NOAA permit approval.

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September 28, 2015: The U.S District Court issues a 100-page document denying the permit application and giving Georgia Aquarium 60 days to decide if they want to appeal that decision.

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November 2015: Out of concern for the welfare of the animals, Georgia Aquarium makes the difficult decision not to appeal the court decision

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April 2016: Although Georgia Aquarium had no legal obligation to continue sending support or supplies after the permit decision, the Aquarium continued to send funds for veterinary supplies and food in its last six months. The Aquarium sent support through the end of April of 2016.

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Facing Extinction Beluga whales in accredited zoological facilities are ambassadors for their species. These whales live in the Arctic; it is cold, remote and expensive to travel to. Seeing beluga whales in person, up close brings marine mammals and conservation education to life for millions of people. These individuals inspire people to care about environmental concerns and become involved in the preservation of the natural world. Currently in the U.S., there are fewer than 30 beluga whales in accredited facilities. These whales are aging, and the population as a whole exhibits poor genetic diversity. In as little as 40 years, there will be no beluga whales in human care in North America. This effectively means that the only belugas millions of children will ever see will be on television or computer screens. Seeing living beluga whales in person has the potential to inspire a generation of people to care about the natural world – and it is this end that aquariums strive to achieve. Without adding new beluga whales to the mix, the dwindling population in human care will become extinct. Georgia Aquarium is deeply committed to both the conservation of beluga whales and providing future generations with the inspiring, educational opportunities that create a culture of preservation.

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Searching the Globe Even prior to opening in 2005, Georgia Aquarium has been committed to provide engaging and transformative experiences that evoke conservation for species. Beluga whales are one such species that need our help more than ever with less than 30 whales in accredited U.S. facilities. In order to increase the number of beluga whales in North America, Georgia Aquarium started the process of searching for unrelated, genetically diverse beluga whales. Mexico City In our efforts to bolster the dwindling population of beluga whales in accredited North American facilities, Georgia Aquarium began to research other countries and their resident beluga whales. This search first led us to an amusement park in Mexico City, where two mature beluga whales were held in a habitat beneath a roller coaster. At this point, the search became a rescue project. Knowing full well one of the Mexican beluga whales was in ill health, Georgia Aquarium transferred them to Atlanta in 2005 to provide better care. Unfortunately, due to their age and already compromised physical condition, they did not live long. The exhibit beneath a roller coaster in Mexico City.

Why Russia? Looking at the different populations of beluga whales residing in the Arctic, it seemed most reasonable to seek out areas that had a robust number of whales, were accessible from a climatology perspective and had historical successes by collectors. Using these parameters, Georgia Aquarium, in collaboration with SeaWorld, Ocean Park Corp. of Hong Kong; Mystic Aquarium in Mystic, Conn.; and Kamogawa Sea World of Japan, chose to begin a population abundance study in Russia. The search continued in Eastern Russia, and Georgia Aquarium sent zoological experts to Chkalov Island where beluga whales are being acquired from the Sea of Okhotsk for marine parks and facilities in Asia. It is important to note that Russia allows an annual catch quota of whales based on the abundance. It was here that our zoological experts also witnessed collection methodologies used by the teams in Russia and these were consistent with those used by NOAA in beluga whale health assessments. The Russian Population of Beluga Whales Russia has more than one stock (population) of beluga whales residing in different bodies of water. The population from which the animals were selected was defined as robust by scientific review. This population will continue to grow, even with the removal of individuals. Before applying for the permit to import these animals, Georgia Aquarium partnered with SeaWorld, Ocean Park Corp. of Hong Kong; Mystic Aquarium in Mystic, Conn.; and Kamogawa Sea World of Japan to conduct an extensive five-year research project on the population abundance and genetic makeup of beluga whales inhabiting the Western Sea of Okhotsk in Russia.

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This research was peer-reviewed and validated in 2011 by the International Union for Conservation of Nature (IUCN), which concluded that the acquisition of these belugas would have no detrimental impact on the existing population. This was a very important and necessary milestone in the project.

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Beluga Whale Population Assessment (2007-2012) Before even applying for a permit to import beluga whales from Russia, Georgia Aquarium wanted to ensure that the population that collections were occurring was stable, and there would be no detrimental impact. To provide accurate and comprehensive information regarding this region of the world that had not been extensively studied before, Georgia Aquarium, in collaboration with SeaWorld, Ocean Park Corp. of Hong Kong; Mystic Aquarium in Mystic, Conn.; and Kamogawa Sea World of Japan, commissioned a fiveyear population study of the Sea of Okhotsk. Funding was provided for scientists from the Russian Academy of Sciences A.N. Severtsov Institute of Ecology and Evolution to conduct this population abundance and genetic study of the beluga whales in the Western Sea of Okhotsk in the Sakhalin-Amur region of Far East Russia. Ensuring Humane Handling of Animals During Study Zoological experts from Georgia Aquarium were sent to Chkalov Island in Eastern Russia to observe collection and release of beluga whales as part of the population abundance and satellite tagging study. The process and techniques witnessed were similar to the methods used by the National Oceanic and Atmospheric Administration (NOAA)/Fisheries and other agencies during cetacean field research studied in North America. Study Concludes Population is Stable After five years of study, the Russian Academy of Sciences A.N. Severtsov Institute of Ecology and Evolution concluded the population of beluga whales in the Sea of Okhotsk was stable. Thus, acquiring a limited number from the population would have no negative impact on its sustainability. International Union for the Conservation of Nature (IUCN) Validates Study Another important step in this process was having the entire study peer-reviewed. The internationallyrecognized authority of wildlife conservation, the International Union for the Conservation of Nature (IUCN), formed an independent scientific review panel. This was led by the Chair of the IUCN Species Specialists Group for Cetaceans, all to peer-review this extensive and comprehensive research. Once the research was reviewed, the IUCN panel concluded the acquisition of 18 beluga whales from the Sea of Okhotsk would have no detrimental impact on the existing population. Georgia Aquarium then set out to apply for an import permit with NOAA Fisheries to bring the beluga whales to the U.S.

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Utrish Marine Station Following the results of the Sea of Okhotsk population abundance study, we felt confident to move forward with plans to acquire 18 beluga whales, knowing that such an acquisition would have no detrimental impact on the population. We began working with Utrish Marine Station, located on the Russian coast of the Black Sea, as a temporary care facility for the whales. The station had a lagoon with sea pens, a rectangular pool and a knowledgeable animal care team. The plan was to house the beluga whales there for a few months before moving them to more contemporary facilities at our accredited partner facility, Ocean Park Hong Kong while we awaited approval to import them to the United States. Georgia Aquarium did not collect these animals. Georgia Aquarium worked to ensure that collection methodologies were done with recognized, professional and humane standards. Our zoological experts witnessed collections in Russia as part of the permit process with NOAA and noted significant similarities between their collection methodologies and those used in NOAA’s standards during beluga whale health assessments.

One of the pools at Utrish Marine Station

The Aquarium collaborated with Utrish Marine Station to care for the 18 beluga whales identified for potential importation to the United States, but does not own the animals, nor does it have any influence related to any other animals managed or cared for at the marine station. There are other marine mammals there for a variety of purposes and they are kept separate from the beluga whales identified for import to the U.S. During our visits to the station activities related to other animals housed there were kept discrete as they involved other affiliates of Utrish unrelated to Georgia Aquarium. Even though Georgia Aquarium does not own the belugas – and never has - we invested heavily in their care. Georgia Aquarium invested approximately $6.5 million, not including legal fees, sending members of our zoological and veterinary teams to Russia an average of twice a year to check on the wellbeing of the animals and to bring additional material support, such as medical supplies and vitamins. Although we decided not to appeal our permit denial in 2015, we continued to support UMS with medical supplies, food, and vitamins for the beluga whales for six months following the project’s end.

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Permit Process March 2011: The International Union for the Conservation of Nature (IUCN) peer-reviews and validates five years of research confirming that the acquisition will have no detrimental impact on the population of beluga whales in the Sea of Okhotsk. June 2012: Georgia Aquarium applies for a permit with the National Oceanic and Atmospheric Administration (NOAA Fisheries) to import beluga whales previously collected from the Sea of Okhotsk to the United States. October 2012: Georgia Aquarium participates in a hearing that allows public comment on the import permit application. More than 15 scientific, academic, conservation and animal welfare experts speak in favor of Georgia Aquarium’s permit request. August 2013: Despite already completing its extensive permit application review process, issuing an environmental assessment report and drafting the permit approval, NOAA Fisheries suddenly changed course and denied the permit application. September 2013: Committed to educating the public about this species, Georgia Aquarium files a complaint in a U.S District Court in Georgia against NOAA Fisheries and the Department of Commerce, seeking to overturn the permit denial. August 2014: Georgia Aquarium files a motion to supplement the record, asking the court to require the release of more than 20 documents deliberately withheld by NOAA Fisheries. These records pertain to NOAA Fisheries’ original decision to approve the import and a complete environmental assessment showing that issuance of the permit complied with the law. The records also include accounts of meetings and discussions that led to the sudden reversal and denial of the permit, and NOAA’s research showing their population counting methods were arbitrary and have never been used before or since. December 2014: The motion to supplement the record is denied. Georgia Aquarium files a motion with the court arguing that -- based on the facts placed before NOAA -- their permit denial was arbitrary, capricious and not in accordance with the law. Georgia Aquarium asks the judge to invalidate the permit denial and to order that the permit to import the whales be issued. August 14, 2015: Georgia Aquarium participates in oral arguments to ask the court to uphold the MMPA and its strong support of zoological institutions, and order issuance of a permit to bring the belugas into the U.S. as soon as possible. September 2015: SeaWorld backs out of beluga import project publically and will no longer take any of the beluga whales destined for U.S. accredited facilities upon pending NOAA permit approval. September 28, 2015: The U.S District Court issues a 100-page document denying the permit application and allowing Georgia Aquarium 60 days to decide whether to appeal that decision. November 17, 2015: Out of concern for the welfare of the animals, Georgia Aquarium makes the difficult decision not to appeal the court decision. April 2016: Georgia Aquarium continued to send infrastructure, veterinary supplies, food, and additional support even without any legal obligation to do so. 12

Difficult Decisions Throughout this process, we’ve had to make many difficult decisions, but we’ve always done what’s right for the animals. Ocean Park Hong Kong backs out. Early in the project, within days of the beluga whales being scheduled to transfer from the temporary facilities at Utrish Marine Station, Ocean Park Hong Kong in Asia backed out of the project due to extremist pressure. The decision by them to withdraw from the project meant the beluga whales would now be staying at Utrish much longer than anticipated. With this change in plans, and the animals’ wellbeing the highest priority, Georgia Aquarium invested approximately $6.5 million, not including legal fees, providing for their care, including building larger pools, food and animal care staff, veterinary medicine and supplies, and our zoological experts and veterinarians visiting the station an average of twice a year to monitor their care, and provide feedback to the Russian team. We maintained regular contact with Russian team throughout the process. If we didn’t fight to bring them to the U.S. where they would receive the highest standard of care, their fate would be uncertain. NOAA Fisheries violated their longstanding interpretation of the Marine Mammal Protection Act, which specifically stresses the importance of caring for animals at zoos and aquariums in order to not only advance science, but to encourage conservation and awareness in the millions of guests who visit these organizations. We feel strongly we were doing what was right and lawful. However, at each point in the legal process, and as we fought their wrongful decision we had to determine whether or not to continue with our appeal. Especially as the animals were being kept longer in what was supposed to only be a temporary location, we were at a crossroads. Concern for the animals’ welfare in Russia The animals’ welfare has always been at the forefront of our minds, and as the legal battle dragged on, we learned of some unfortunate health and wellness issues affecting a few of the beluga whales selected for potential import to the U.S. Between 2013 and 2015, there were four unfortunate losses from a variety of causes that are not fully understood because necropsies were not conducted and our staff was not present. We believe the health of a couple of these animals were compromised by long term management of the animals at a temporary facility. The Russian team does not have the contemporary resources we have at accredited facilities which allow for timely response to health issues as well as easy separation of animals for medical purposes. The belugas need a permanent home in quality facilities After two years of court deliberation, and although we felt strongly we were in the right, we realized any further legal action would be fruitless. It wasn’t in the best interest of the 15 remaining animals to stay at the Russian facility for another year or more of litigation. We made the difficult choice to not appeal the decision, and we accepted the fact that these belugas will never be allowed to live within an accredited facility here in the United States. But it now means these animals have an uncertain future.

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An Uncertain Future for the Belugas in Russia The beluga whales once destined for accredited North American aquariums and marine parks must now find homes at zoological institutions in other parts of the world. Many will likely end up in areas where animal care standards are not always as high as those found in the United States. While this is not ideal, it is the best option still available. But we are doing everything possible to advocate for their relocation to contemporary, quality facilities where their care and welfare is a priority. Georgia Aquarium does not own the animals that reside at Utrish Marine Station, nor have any economic gain or control over where they go. But we are doing everything possible to advocate for their relocation to contemporary, quality facilities where their care and welfare is a priority. The welfare of these animals has been our utmost priority throughout this entire process. It is why we spent approximately $6.5 million, not including legal fees, to care for and support these animals. An Uncertain Future for Belugas in North America and Around the World There are now fewer than 30 beluga whales left in accredited U.S. zoological facilities, and it is estimated that by 2050 there may not be any. Without adding to this group of whales, the days of belugas in human care are numbered. Your children and grandchildren may never have a chance to see these magnificent animals up close. Belugas represent the Arctic – a place most of us will never get to see, and an area of the world that is drastically changing as the polar icecap melts. Without belugas in human care to serve as ambassadors for their species, future generations will not have the chance to create powerful, personal connections with these animals. These connections are critical to inspiring people to take action toward the preservation of this species and their natural environment. Beluga whales in human care also provide us many research opportunities that would be impossible to pursue with belugas in their natural environment. What we learn from belugas in our care helps inform conservation decisions for beluga whales worldwide. Maintaining a population of belugas in accredited zoos and aquariums is essential to the conservation of the species in its natural habitat. Georgia Aquarium will continue our commitment to ensuring a sustainable population of beluga whales in accredited North American aquariums and marine parks and to protecting and preserving this species for generations to come.

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Conservation The conservation of beluga whales, and all animals, is at the core of Georgia Aquarium’s mission and passion. As a global leader in marine research and conservation, we remain steadfast in our commitment to contribute to wildlife sustainability through: - Education initiatives inspiring others to conserve the species and its natural habitat - Participating in cooperative breeding and research programs among North America’s accredited zoological institutions, applying known and successful practices to build a sustainable population - Conservation and research of animals in human care and their natural environment - Sharing our knowledge and experiences with the international scientific and conservation community We have supported and funded more than 100 marine conservation programs in ten years, including animal health assessments. Two primary marine mammal conservation programs in which Georgia Aquarium participates include Bristol Bay Beluga Project in Alaska and the Health and Environmental Risk Assessment (HERA) for Atlantic bottlenose dolphins in Florida. Bristol Bay Beluga Project By studying and observing beluga whales in human care, we are able to create baseline indicators to understand issues threatening beluga whales in the wild. Our animal care team’s daily interaction with our resident beluga whales helps researchers better understand how to approach and interact with whales in the ocean in a controlled way. Our daily work with these animals at the Aquarium makes assessing, and ultimately helping, wild populations possible. Since 2008, Georgia Aquarium has participated in a collaborative study assessing the health of beluga whales in Bristol Bay, Alaska, where the beluga population faces significant threats to their natural environment. We have gained insight into the effects of underwater sound levels on belugas, how they respond to increasing pathogens and changing water temperatures in the wild, and nutritional needs of belugas that face increasing challenges for food sources.

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Through the combination of the Bristol Bay Beluga Project and our studies in human care, we provide crucial information to researchers and the zoological community. Our goal is to use this vital information gathered from the Bristol Bay Beluga Project to help identify ways to potentially alleviate threats facing the critically endangered beluga whale population of nearby Cook Inlet in Alaska. Our work doesn’t just end with helping one geographic population – we’re marine mammal advocates; our continued work ensures the protection of beluga whale populations globally. HERA for Atlantic Bottlenose Dolphins Initiated by Georgia Aquarium in 2003, HERA is a comprehensive program that assesses and studies potential environmental and human-induced stressors that may affect the health and long-term viability of bottlenose dolphin populations. This groundbreaking research takes place in the Indian Lagoon River in Florida and the coastal waters of South Carolina, where researchers can gain valuable information concerning zoonotic diseases, contaminant issues and other factors such as antibiotic-resistant bacteria that have huge implications for human health. HERA plays an important role in understanding the health of Atlantic bottlenose dolphins and the possible threats facing the lagoon ecosystem. Applying our expert animal and veterinary care at the Aquarium into the field helps maximize the research collected from dolphin health assessments and ultimately ensure that these magnificent marine mammals thrive in their natural environment.

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GEORGIA AQUARIUM EXPERT BIOS AND RESEARCH BIBLIOGRAPHIES

DR. GREGORY BOSSART, V.M.D, PH.D., SENIOR VICE PRESIDENT, CHIEF VETERINARY OFFICER ERIC GAGLIONE, VICE PRESIDENT OF ZOOLOGICAL OPERATIONS

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Dr. Gregory Bossart, V.M.D., Ph.D. Senior Vice President, Chief Veterinary Officer, Georgia Aquarium Dr. Gregory Bossart has spent the last 30 years working in clinical domestic, marine mammal and avian medicine and wildlife pathology on a national and international basis. He has over 135 peer-reviewed scientific publications focused primarily on the pathologic basis of disease in wild animals. Dr. Bossart received his doctorate in veterinary medicine from the University of Pennsylvania. He was a comparative pathology resident and National Institutes of Health fellow in the Department of Pathology at the University of Miami School of Medicine. In 1995, he completed his Ph.D. in immunology at Florida International University. He has been in private veterinary practice and is a clinical veterinary consultant at facilities in the United States, Latin America and Asia. Presently, Dr. Bossart is Senior Vice President and Chief Veterinary Officer at Georgia Aquarium in Atlanta, Georgia where he oversees the animal care, research and conservation programs. He is an Adjunct Professor in the Department of Pathology at the University Of Miami School Of Medicine, Adjunct Professor in the Department of Pathology at the University Of Georgia College Of Veterinary Medicine, Research Professor at Harbor Branch Oceanographic Institute at Florida Atlantic University and on the graduate faculty at the Medical University of South Carolina. His recently published research has documented re-surging and emerging diseases in manatees, whales, dolphins, and birds. He has helped characterize the first viral disease in manatees and was responsible for developing the first immunohistochemical technique for diagnosing red tide poisoning in marine mammals and birds. He is particularly interested in the application of aquatic species as sentinels for the effects of global climate change, ecosystem and human health. Dr. Bossart has been presented multiple awards and accolades for his work. Examples of Dr. Bossart’s research can be found in the Journal of the American Veterinary Medical Association, Veterinary Pathology, Journal of Zoo and Wildlife Medicine, Veterinary Record, Journal of Avian Medicine and Surgery, Toxicologic Pathology, Marine Mammal Science, Experimental and Molecular Pathology, Aquatic Mammals, Florida Scientist, Journal of Raptor Research, Journal of Veterinary Diagnostic Investigation, Oceanography, Journal of Wildlife Diseases, Environmental Science & Technology, Journal of Parasitology, Aquatic Toxicology and Veterinary Microbiology and Nature.

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Dr. Gregory Bossart V.M.D., Ph.D. Georgia Aquarium Publications and Abstracts 2006-Present 2007 1. Bossart GD, Hansen L, Goldstein J, Kilpatrick D, Bechdel S, Howells E, Kroell K, de Sieyes M, Stolen M, Noke Durden W, Reif J, Defran R, and McCulloch S. Pathological findings in a rare mass stranding of melon-headed whales (Peponcephala electra) in Florida. Aquatic Mammals. 33(2): 235-240, 2007. 2. Bossart GD, Hensley G, Goldstein J, Kroell K, Manire C, Defran R, and Reif J. Cardiomyopathy and myocardial degeneration in stranded pygmy (Kogia breviceps) and dwarf sperm (Kogia sima) whales. Aquatic Mammals. 33(2): 214-222, 2007. 2008 3. Adams J, Houde M, Muir D, Speakmand T, Bossart GD, Fair PA. Land use and the spatial distribution of perfluoroalkyl compounds as measured in the plasma of bottlenose dolphins (Tursiops truncatus). Marine Environmental Research. 66: 430-437, 2008. 4. Bossart GD, Romano TA, Peden-Adams MM, Rice CD, Fair PA, Goldstein JD, Cammen K, and Reif JS. Hematological, biochemical and immunological findings in Atlantic bottlenose dolphins (Tursiops truncatus) with orogenital papillomas. Aquatic Mammals. 34(2): 166-177, 2008. 5. Fayer R, Fair PA, Bossart GD, Santin M. Examination of naturally exposed bottlenose dolphins (Tursiops truncatus) for microsporidia, Cryptosporidium, and Giardia. Journal of Parasitology. 94(1): 143-147, 2008. 6. Mazzoil M, Reif JS, Youngbluth M, Murdoch EM, Bechdel SE, Howells E, McColluch SD, Hansen LJ, Bossart GD. Home Ranges of Bottlenose Dolphins (Tursiops truncatus) in the Indian River Lagoon, Fl. EcoHealth. 5: 278-288, 2008. 7. Mazzoil MS, Kilpatrick DS, Murdoch ME, Mase-Guthrie B, Odell DK, Bossart GD. Radio-tracking and survivorship of two rehabilitated bottlenose dolphins (Tursiops truncatus) in the Indian River Lagoon. Aquatic Mammals. 34(1): 54-64, 2008. 8. Montie EW, Fair PA, Bossart GD, Mitchum GB, Houde M, Muir DCG, Letcher RJ, McFee WE, Starczak VR, Stegeman JJ, Hahn ME. Cytochrome P4501A1 expression, polychlorinated biphenyls and hydroxylated metabolites, and adipocyte sixe of bottlenose dolphins from the southeast United States. Aquatic Toxicology. 86: 397-412, 2008. 9. Murdoch ME, Reif JS, Mazzoil M, McCulloch SD, Fair PA, Bossart GD. Lobomycosis in bottlenose dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida: Estimation of prevalence, temporal trends, and spatial distribution. EcoHealth. 5: 289-297, 2008. 10. Rector A, Stevens H, Lacave G, Lemey P, Mostmans S, Salbany A, Vos M, Van Doorslaer K, Ghim SJ, Rehtanz M, Bossart GD, Jenson AB, Van Ranst M. Genomic characterization of novel dolphin papillomaviruses provides indications for recombination within the Papillomavairidae. Virology. 378: 151-161, 2008.

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11. Rehtanz M, Bossart GD, Doescher B, Rector A, Van Ranst M, Fair P, Jenson AB, Ghim S. Bottlenose dolphin (Tursiops truncatus) papillomaviruses: Vaccine antigen candidates and screening test development. Veterinary Microbiology. DOI: 10.1016/j.vetmic.2008.06.017, 2008. 12. Reif JS, Fair PA, Adams J, Joseph B, Kilpatrick DS, Sanchez R, Goldstein JD, Townsend FI, McCulloch SD, Mazzoil M, Zolman ES, Hansen LJ, Bossart GD. Evaluation and comparison of the health status of Atlantic bottlenose dolphins from the Indian River Lagoon, Florida and Charleston, South Carolina. Journal of the American Veterinary Association. 233: 299-307, 2008. 13. Stavros HCW, Bossart GD, Hulsey TC, Fair PA. Trace element concentrations in blood of freeranging bottlenose dolphins (Tursiops truncatus): influence of age, sex and location. Marine Pollution Bulletin. 56: 348-379, 2008. 2009 14. Bechdel SE, Mazzoil MS, Murdoch ME, Howells EM, Reif JS, McCulloch SD, Schaefer AM, Bossart GD. Prevalence and impacts of motorized vessels on bottlenose dolphins (Tursiops truncatus) in the Indian River Lagoon, Florida. Aquatic Mammals. 35: 367-377, 2009. 15. Bossart GD. Emerging diseases in marine mammals (Book Chapter). McGraw-Hill Yearbook of Science and Technology, McGraw-Hill Co., New York. pp: 103-105, 2009. 16. Fair PA, Lee HB, Adams J, Darling C, Pacepavicius MA, Bossart GD, Henry N, Muir D. Occurrence of triclosan in plasma of wild Atlantic bottlenose dolphins (Tursiops truncatus) and their environment. Environmental Pollution. 157: 2248-2254, 2009. 17. Fire SE, Wang Z, Leighfield TA, Morton SL, McFee WE, McLellan WA, Litaker RW, Tester PA, Hohn AA, Lovewell G, Harms C, Rotstein DS, Barco SG, Costidis A, Sheppard B, Bossart GD, Stolen M, Durden WN, Van Dolah FW. Domoic acid exposure in pygmy and dwarf sperm whales (Kogia spp) from southeastern and mid-Atlantic US waters. Harmful Algae. 8: 658-664, 2009. 18. Houde M, Pacepavicius G, Darling C, Fair PA, Alaee M, Bossart GD, Solomon KR, Letcher RJ, Bergman A, Marsh G, Muir DCG. Polybrominated diphenyl ethers and their hydroxylated analogs in plasma of bottlenose dolphins (Tursiops turncatus) from the United States east coast. Environmental Toxicology and Chemistry. 28(10): 2061-2068, 2009. 19. Howells EM, Reif JS, Bechdel SE, Murdoch ME, Bossart GD, McCulloch SD, Mazzoil, MS. A novel case of non-offspring adoption in a free-ranging Atlantic bottlenose dolphin (Tursiops truncatus) inhabiting the Indian River Lagoon, Florida. Aquatic Mammals. 35 (1): 43-47, 2009. 20. Johnson WR, Tarralba M, Fair PA, Bossart GD, Nelson K, Morris PJ. Novel diversity of bacterial communities associated with bottlenose dolphin upper respiratory tracts. Environmental Microbiology Reports. 1(6): 55-562, 2009. 21. Mignucci-Giannoni AA, Rosario-Delestre RJ, Alsina-Guerreron MM, Falcón-Matos L, GuzmánRamírez L, Williams EH, Bossart GD, Reidenberg JS. Asphyxiation in a bottlenose dolphin (Tursiops truncatus) from Puerto Rico due to choking on a black margate (Anisotremus surinamensis). Aquatic Mammals 35(1): 48-54, 2009.

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22. Mollenhauer MAM, Carter BJ, Peden-Adams MM, Bossart GD, Fair PA. Gene expression changes in bottlenose dolphin (Tursiops truncatus) skin cells following exposure to methylmercury (MeHg) or perfluorrooctane sulfonate (PFOS). Aquatic Toxicology. 91: 10-18, 2009. 23. Rehtanz M, Bossart GD, Doescher B, Rector A, Van Rast M, Fair PA, Jenson AB, Ghim SJ. Bottlenose Dolphin (Tursiops truncatus) papillomaviruses: Vaccine antigen candidates and screening test development. Veterinary Microbiology. 133: 43-53, 2009. 24. Reif JS, Peden-Adams MM, Romano TA, Rice CD, Fair PA, Bossart GD. Immune dysfunction in Atlantic bottlenose dolphins (Tursiops truncatus) with lobomycosis . Medical Mycology. 47: 125135, 2009. 25. Schaefer AM, Reif JS, Goldstein JD, Ryan CN, Fair PA, Bossart GD. Serological evidence of exposure to selected pathogens in free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida and Charleston, South Carolina. Aquatic Mammals. 35: 163-170, 2009. 26. Schaefer, AM, Goldstein JD, Reif JS, Fair PA, Bossart GD, Antibiotic-resistant organisms cultured from Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting estuarine waters of Charleston SC and Indian River Lagoon, Fl. EcoHealth. 6: 33-41, 2009. 27. Schwacke LH, Hall AJ, Townsend FI, Wells RS, Hansen LJ, Hohn AA, Bossart GD, Fair PA, Rowles TK. Hematologic and serum biochemical reference intervals for free-ranging bottlenose dolphins (Tursiops truncatus) and variation in the distributions of clinicopathologic values related to geographic sampling site. American Journal of Veterinary Research. 70(8): 973-985, 2009. 2010 28. Bossart GD, Reif JS, Schaefer AM, Goldstein J, Fair PA, Saliki JT. Morbillivirus infection in freeranging Atlantic bottlenose dolphins (Tursiops truncatus) from the southeastern United States: Seroepidemiologic and pathologic evidence of subclinical infection. Veterinary Microbiology 143: 160-166, 2010. 29. Fair P, Adams J, Mitchum G, Hulsey T, Reif J, Houde M, Muir D, Wirth E, Wetzel D, Zolman E, McFee W, Bossart GD. Contaminant blubber burdens in Atlantic bottlenose dolphins (Tursiops truncatus) from two southeastern US estuarine areas: Concentrations and patterns of PCBs, pesticides, PBDEs, PFCs, and PAHs. Science of the Total Environment. 408: 1577-1597, 2010. 30. Meegan J, Field C, Sidor I, Romano T, Casinghino S, Smith CR, Kashinsky L, Fair PA, Bossart GD, Wells R, Dunn JL. Development, validation, and utilization of a competitive enzyme-linked immunosorbent assay for the detection of antibodies against Brucella species in marine mammals. Journal of Veterinary Diagnostic Investigation. 22: 856-862, 2010. 31. Rehtanz M, Ghim SJ, McFee W, Doescher B, Lacave G, Fair PA, Reif JS, Bossart GD, Jenson AB. Papillomaviruses antibody prevalence in free-ranging and captive bottlenose dolphins (Tursiops truncatus). Journal of Wildlife Diseases. 46(1): 136-145, 2010. 32. Staggs L, St. Leger J, Bossart GD, Townsend Jr. FI, Hicks C, Rinaldi M. A novel case of Fusarium oxysporum infection in an Atlantic bottlenose dolphin (Tursiops truncatus). Journal of Zoo and Wildlife Medicine. 41(2): 287-290, 2010.

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2011 33. Bossart GD, Romano T, Peden-Adams M, Schaefer A, McCulloch S, Goldstein J, Rice C, Saliki J, Fair P, Reif JS. Clinicoimmunopathologic findings in Atlantic bottlenose dolphins (Tursiops truncatus) with positive morbillivirus titers. Diseases of Aquatic Organisms. 97: 103–112, 2011. 34. Bossart, GD. Marine mammals as sentinel species for oceans and human health. Veterinary Pathology. 48(3): 676-690, 2011. 35. Dona MG, Rehnatz M, Adimey NM, Bossart GD, Jenson AB, Bnde RK, Ghim SJ. Seroepidemiology of TmPV1 infection in captive and wild Florida manatees (Trichechus manatus latirostris). Journal of Wildlife Diseases. 47(3): 673-684, 2011. 36. Mazzoil M, Murdoch E, Reif JS, Bechdel SE, Howells E, De Sieyes M, Lawrence C, Bossart GD, McCulloch SD. Site fidelity and movement of bottlenose dolphins (Tursiops truncatus) on Florida’s east coast: Atlantic Ocean and Indian River Lagoon estuary. Florida Scientist. 74(1): 2537, 2011. 37. Morris PJ, Johnson WR, Pisani J, Bossart GD, Adams J, Reif JS, Fair PA. Isolation of culturable microorganisms from free-ranging bottlenose dolphins (Tursiops truncatus) from the southeastern United States. Veterinary Microbiology. 148: 440-447, 2011. 38. Murdoch ME, Mazzoil M, McCulloch S, Bechdel S, O’Corry-Crowe G, Bossart GD, Reif JS, Lacaziosis in bottlenose dolphins (Tursiops truncatus) along the coastal Atlantic Ocean, Florida USA. Diseases of Aquatic Organisms. 92: 69-73, 2011. 39. Schaefer AM, Stavros HCW, Bossart GD, Fair PA, Goldstein JD, Reif JS. Association between mercury and hepatic, renal, endocrine, and hematological parameters in Atlantic bottlenose dolphins (Tursiops truncatus) along the eastern coast of Florida and South Carolina. Archives of Environmental Contamination and Toxicology. 61: 688-695, 2011. 40. Stavros HCW, Stolen M, Durden WN, McFee W, Bossart GD, Fair PA. Correlation and toxicological inference of trace elements in tissues from stranded and free-ranging bottlenose dolphins (Tursiops truncatus). Chemosphere. 82: 1649-1661, 2011. 2012 41. Bonde RK, Mignucci-Giannoni A, Bossart GD. Sirenian Pathology and Mortality Assessment (Book Chapter). Sirenian Conservation: Issues and Strategies in Developing Countries. (Hines, Reynolds, Aragones, Mignucci-Giannoni, Marmontel, eds.). University Press of Florida, Gainesville, Florida. pp: 148-156, 2012. 42. Bossart GD, Arheart K, Hunt M, Clauss T, Leppert L, Roberts K, McCulloch S, Goldstein JD, Gonzalez C, Sweeney J, Stone R, Fair PA, Cray C. Protein electrophoresis of serum from healthy Atlantic bottlenose dolphins (Tursiops truncatus). Aquatic Mammals. 38(4): 412-417, 2012. 43. Bossart GD, Mignucci-Giannoni AA, Rivera-Guzman AL, Jimenez-Marrero NM, Camus AC, Bonde RK, Dubey JP, Reif JS. Disseminated toxoplasmosis in Antillean manatees (Trichechus manatus manatus) from Puerto Rico. Diseases of Aquatic Organisms. 101: 139-144, 2012.

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44. Dove ADM, Leisen J, Zhou M, Byrne JJ, Lim-Hing K, Webb HD, Gelbaum L, Viant, MR, Kubanek J, Fernandez F. Biomarkers of whale shark health: A metabolomic approach. PLoS ONE. 7(11): 110, 2012. 45. Fair PA, Houde M, Hulsey TC, Bossart GD, Adams J, Balthis L, Muir DCG. Assessment of perflourinated compounds (PFCs) in plasma of bottlenose dolphins from two southeast US estuarine areas: Relationship with age, sex and geographic locations. Marine Pollution Bulletin. 64: 66-74, 2012. 46. Fair PA, Stavros HC, Mollenhauer M, DeWitt J, Henry N, Kannan K, Yun SH, Bossart GD, Keil K, Peden-Adams M. Immune function in female B6C3F1 mice is modulated by DE-71, a commercial polybrominated diphenyl ether mixture. Journal of Immunotoxicology. 9(1): 96–107, 2012. 47. Goldstein JD, Shaefer AM, McCulloch SD, Fair PA, Bossart GD, Reif JS. Clinicopathologic findings from Atlantic bottlenose dolphins (Tursiops truncatus) with cytologic evidence of gastric inflammation. Journal of Zoo and Wildlife Medicine. 43(4): 730-738, 2012. 48. Mazzaro LM, Johnson SP, Fair PA, Bossart GD, Carlin KP, Jensen ED, Smith CR, Andrews GA, Chavey PS, Venn-Watson S. Iron Indices in bottlenose dolphins (Tursiops truncatus). Comparative Medicine. 62(6): 508-515, 2012. 49. McFee WE, Adams JD, Fair PA, Bossart GD. Age distribution and growth of two Bottlenose dolphin (Tursiops truncatus) populations from capture-release studies in the southeastern United States. Aquatic Mammals. 38(1): 17-30, 2012. 50. Rehtanz M, Bossart GD, Fair PA, Reif JS, Ghim SJ, Jenson AB. Papillomaviruses and herpesviruses: Who is who in genital tumor development of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus)? Veterinary Microbiology. 160: 297-304, 2012. 2013 51. Bossart GD, Hurley W, Biedenbach G, Denny M, Borkowski R, Goricki C, Searcy E, Roberts K, Reif J. Pathologic findings in stranded cetaceans from northeastern Florida. Florida Scientist. 76(1): 36-50, 2013. 52. Bergfelt DR, Steinetz BG, Reif JS, Schaefer AM, Bossart GD, Mazzoil MS, Zolman E, Fair PA. Evaluation of single-sample analysis of progesterone in combination with relaxin for diagnosis of pregnancy in wild bottlenose dolphins (Tursiops truncatus). Aquatic Mammals. 39(2): 198-206, 2013. 53. Cray C, Arheart KL, Hunt M, Clauss TM, Leppert LL, Roberts K, McCulloch SD, Goldstein JD, Gonzalez C, Sweeney J, Stone R, Fair PA, Bossart GD. Acute phase protein quantitation in serum samples from healthy Atlantic bottlenose dolphins (Tursiops truncatus). Journal of Veterinary Diagnostic Investigation. 25(1): 107-111, 2013. 54. Hickie BE, Cadieux MA, Riehl KN, Bossart GD, Alava JJ, Fair PA. Modeling PCB-bioaccumulaiton in the bottlenose dolphin (Tursiops truncatus): Estimating a dietary threshold concentration. Environmental Science and Technology. DOI: 10.1021/es403166b, 2013. 55. Lee RF, Bulski K, Adams JD, Peden-Adams M, Bossart GD, King L, Fair PA. DNA strand breaks (comet assay) in blood lymphocytes from wild bottlenose dolphins. Marine Pollution Bulletin. DOI: 10.1016/j.marpolbul.2013.06.017, 2013. 23

56. Lewis L, Lamb SV, Schaefer AM, Reif JS, Bossart GD, Fair PA. Influence of collection and storage conditions of Adrenocorticotropic hormone (ACTH) measurements in bottlenose dolphins (Tursiops truncatus). Aquatic Mammals. 39(4): 324-329, 2013. 57. Reif JS, Schaefer AM, Bossart GD. Lobomycosis: Risk of zoonotic transmission from dolphins to humans. Vector Borne Zoonotic Diseases. 13(10): 689-693, 2013. 58. Richards VP, Greig TW, Fair PA, McCulloch SD, Politz C, Natoli A, Driscoll CA, Hoelzel AR, David V, Bossart GD, Lopez JV. Patterns of population structure for inshore bottlenose dolphins along the eastern United States. Journal of Heredity. 104: 765-778, 2013. 59. Wirth JR, Peden-Adams MM, White ND, Bossart GD, Fair PA. In Vitro PFOS exposure on immune endpoints in bottlenose dolphins (Tursiops truncatus) and mice. Journal of Applied Toxicology. DOI 10.1002/jat.2891, 2013. 2014 60.

61.

62.

63.

64.

65.

66.

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Bossart GD, Romano T, Peden-Adams M, Schaefer A, McCulloch S, Goldstein J, Fair P, Cray C, Reif JS. Clinicoimmunopathologic findings in Atlantic bottlenose dolphins (Tursiops truncatus) with positive Chlamydiaceae antibodies. Diseases of Aquatic Organisms. 2014. Browning NE, McCulloch SD, Bossart GD, Worthy AJ. Fine-scale population structure of estuarine bottlenose dolphins (Tursiops truncatus) assessed using stable isotope ratios and fatty acid signature analyses. Marine Biology DOI 10.1007/s00227-014-2420-z, 2014. Wirth JR, Peden-Adams MM, White ND, Bossart GD, Fair PA. In vitro exposure of DE-71, a pentaPBDE mixture on immune endpoints in bottlenose dolphins (Tursiops truncatus) and B6C3F1 mice. Journal of Applied Toxicology. DOI 10.1002/jat.3008. 2014. Ghim S, Joh J, Mignucci-Giannoni AA, Rivera-Guzman AL, Falcon-Matos L, Alsina-Guerrero MM, Rodriguez-Villanueva M, Jenson AB, Bossart GD. Genital Papillomatosis Associated with Two Novel Mucosotropic Papillomaviruses from a Florida manatee (Trichechus manatus latirostris). Aquatic Mammals. 40(20): 189-194. 2014. Mealey BK, Baldwin JD, Parks-Mealey GB, Bossart GD, Forstner MRJ. Characteristics of mangrove diamondback terrapins (Malaclemys terrapin rhizophorarum) inhabiting altered and natural mangrove islands. Journal of North American Herpetology. 2014(1): 76-80. 2014. Schaefer AM, Jensen EL, Bossart GD, Reif JS. Hair Mercury Concentrations and Fish Consumption Patterns in Florida Residents. International Journal of Environmental Research and Public Health. 11: 6709-6726. DOI:10.3390/ijerph110706709. 2014. Fair PA, Balthis L, Kannan, K, De Silva A, Wu Q, French KM, Houde M, Muir DCG, Daugomah J, Spencer C, Bossart GD, White ND. Dolphins as sentinels for sediment perflouroalkyl substances (PFASs) contamination in estuarine areas of Charleston, SC. Poster session: SETAC North America. 35th Annual Meeting; 2014 November 12; Vancouver, BC Canada. Farquier D, Goldstein T, Colegrove K, Rotstein D, DiGiovanni Jr. RA, McLellan W, Northeast and Southeast Atlantic Marine Mammal Stranding Network, Habecker P, Coffee L, Howerth EW, Gottdenker N, Bossart G, St. Leger J, Waltzek TB, Wellehan J, Saliki, JT, Nielsen O, Garron M, Mase-Guthrie B, Rowles TK. Dolphin Morbillivirus Outbreak and the 2013-2014 Mid-Atlantic

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Bottlenose Dolphin Unusual Mortality Event. 63rd Annual International Conference of the Wildlife Disease Association. July 27-August 1, 2014. Albuquerque, New Mexico. Fair PA, Schaefer AM, Romano TM, Bossart GD, Lamb SV, Reif JS. Stress response of wild bottlenose dolphins (Tursiops truncatus) during capture-release assessment studies. General and Comparative Endocrinology. 206: 203-212. 2014. Browning NE, McCulloch SD, Bossart GD, Worthy GAJ. Fine-scale population structure of estuarine bottlenose dolphins (Tursiops truncatus) assessed using stable isotope ratios and fatty acid signature analyses. Marine Biology. DOI 10.1007/s00227-014-2420-z. 2014.

2015 70.

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Fauquier DA, Goldstein T, Colegrove KM, Rotstein DS, DiGiovanni Jr. RA, McLellan WA, Northeast and Southeast Marine Mammal Stranding Network, Habecker P, Coffee L, Swist S, Howerth EW, Gottdenker N, Bossart GD, St. Leger J, Waltzek TB, Wellehan T, Saliki JT, Nielsen O, Garron M, Mase-Guthrie B, Rowles TK. The 2013-2014 Mid-Atlantic Bottlenose Dolphin (Tursiops truncatus) Unusual Mortality Event and Dolphin Morbilivirus. 46th Annual International Association for Aquatic Animal Medicine Conference. April 6-10, 2015. Chicago, Illinois. Schaefer AM, Murdoch Titcomb E, Jensen EL, Fair PA, Stavros HW, Mazzoil M, Bossart GD, Reif JS. Mercury concentrations in Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting the Indian River Lagoon, Florida and local human residents: Patterns of distribution. 5th Florida Marine Mammal Health Conference, Gainesville, Fl. June 2015. Fauquier DA, Goldstein T, Colegrove KM, Rotstein DS, DiGiovanni Jr. RA, McLellan WA, Northeast and Southeast Atlantic Marine Mammal Stranding Network, Rosel P, Wilcox L, Stolen M, Urian K, Habecker P, Coffee L, Swist S, Howerth EW, Gottdenker N, Bossart G, St. Leger J, Waltzek TB, Wellehan JFX, Saliki JT, Nielsen O, Morris SE, Zelner J, Grenfell BT, Garron M, Mase-Guthrie B, Rowles TK. Dolphin Morbilivirus and the 2013-2015 Mid-Atlantic bottlenose dolphin (Tursiops truncatus) Unusual Mortality Event. 21st biennial Conference on Marine Mammals. San Francisco, CA. December 13-18, 2015. McCulloch SD, Goldstein JD, Walsh M, Pelton C, Bossart GD. Cost, Benefits and Solutions An Overview of Planning and Executing Marine Mammal Health and Environmental Risk Assessments and Interventions. 5th Florida Marine Mammal Health Conference. Gainesville, FL. June 2-4, 2015. Bossart GD, Schaefer AM, McCulloch S, Goldstein J, Fair PA, Reif JS. Mucocutaneous lesions from free-ranging Atlantic bottlenose dolphins, Tursiops truncatus, from the southeastern United States. Diseases of Aquatic Organisms. 115:175-184. 2015. Reif JS, Schaefer AM, Bossart GD. Atlantic bottlenose dolphins (Tursiops truncatus) as a sentinel for exposure to mercury in humans: closing the loop. Veterinary Sciences. (2)407-422: doi:10.3390/vetsci2040407. 2015.

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76.

77.

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Schaefer AM, Titcomb EM, Fair PA, Stavros HC, Mazzoil M, Bossart GD, Reif JS. Mercury concentrations in Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting the Indian River Lagoon, Florida: Patterns of spatial and temporal distribution. Marine Poll Bull. 97: 544-547. 2015. Jaing C, Thissen JB, Gardner S, McLoughlin K, Slezak T, Bossart GD, Fair PA. Pathogen surveillance in wild bottlenose dolphins Tursiops truncatus. Diseases of Aquatic Organisms. 116:83-91. 2015 Cray, C., Rodriguez, M., Field, C., McDermott, A., Leppert, L., Clauss, T., Bossart, G.D. Protein and cholesterol electrophoresis of plasma samples from captive cownose ray (Rhinoptera bonasus). Journal of Veterinary Diagnostic Investigation. 27(6): 688-695. 2015.

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Eric Gaglione Vice president of zoological operations, Georgia Aquarium Eric Gaglione began his career in 1984 as a sea lion and dolphin trainer at the Aquarium of Niagara Falls while also earning degrees in Zoology and Wildlife Management at the State University of New York’s Empire State College. He assumed the role of Curator of Marine Mammals in 1990 and spent five years overseeing the aquarium’s animal training and behavioral enrichment programs. In 1995 he joined the Mystic Aquarium as Curator of Marine Mammals & Birds. While at Mystic he gained valuable experience with beluga whales and a variety of marine mammals and birds including bottlenose dolphins, California sea lions, Steller sea lions, Northern fur seals, harbor seals, grey seals, Pacific walrus and African penguins. He was also responsible for the on-site animal care of Mystic’s stranded marine mammal program and cared for a variety of seal and cetacean species including but not limited to pilot whales, Risso’s dolphins, common dolphins, harbor porpoise, hooded seals, harp seas and harbor seals. In 2005, Gaglione joined the Georgia Aquarium and initially managed the Cold Water Quest Gallery where he assisted the start-up of the one the largest aquariums in the world. Gaglione has continued to specialize in beluga whale management, training and care and further diversified his experience with marine animals including whale sharks. He has served in numerous capacities in the zoological division and is currently Vice President of Zoological Operations. He is responsible for the management, training and care of the mammal and bird collections in the aquarium exhibit galleries which currently include beluga whales, bottlenose dolphins, California sea lions, harbor seals, southern sea otters, Asian otters and African penguins. Gaglione has an extensive history caring for beluga whales for the past twenty-one years. He has also participated in field research projects and health assessments involving beluga whales in the Hudson Bay Nelson River estuary and St. Lawrence River, Canada as well as Bristol Bay, Alaska. He is involved with several groups and associations related to the care of marine animals. He has been and continues to be a representative for Georgia Aquarium with the Alliance of Marine Mammal Parks and Aquariums (AMMPA). He is a Professional Associate of the Association of Zoos and Aquariums (AZA) and is member of AZA’s Marine Mammal Taxon Advisory Group Steering Committee. He has been a member of the International Marine Animal Trainers’ Association (IMATA) since 1985 and served on the board for six years including Vice President (2010) and President (2013).

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Chapter 88 Measuring Hearing in Wild Beluga Whales T. Aran Mooney , Manuel Castellote , Lori Quakenbush , Roderick Hobbs ,Caroline Goertz , and Eric Gaglione Abstract We measured the hearing abilities of seven wild beluga whales (Delphinapterus leucas) during a collection-and-release experiment in Bristol Bay, AK. Here we summarize the methods and initial data from one animal and discuss the implications of this experiment. Audiograms were collected from 4 to 150 kHz. The animal with the lowest threshold heard best at 80 kHz and demonstrated overall good hearing from 22 to 110 kHz. The robustness of the methodology and data suggest that the auditory evoked potential audiograms can be incorporated into future collection-and-release health assessments. Such methods may provide high-quality results for multiple animals, facilitating population-level audiograms and hearing measures in new species.

Keywords Anthropogenic noise • Sensory • Marine mammal • Cetacean •Odontocete • Arctic T. A. Mooney Biology Department , Woods Hole Oceanographic Institution , 266 Woods Hole Road, MRF MS50 , Woods Hole , MA 02543 , USA e-mail: [email protected] M. Castellote National Marine Mammal Laboratory , Alaska Fisheries Science Center, National Marine Fisheries Service , Seattle , WA 98115 , USA North Gulf Oceanic Society , Homer , AK 99603 , USA e-mail: [email protected] L. Quakenbush Alaska Department of Fish and Game , Fairbanks , AK 99701 , USA e-mail: [email protected] R. Hobbs National Marine Mammal Laboratory , Alaska Fisheries Science Center, National Marine Fisheries Service , Seattle , WA 98115 , USA C. Goertz Alaska SeaLife Center , Seward , AK 99664 , USA e-mail: [email protected] E. Gaglione Georgia Aquarium , 225 Baker Street NW , Atlanta , GA 30313 , USA e-mail: [email protected]

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1 Introduction Hearing is the primary sensory modality for odontocete marine mammals. They are generally considered to have sensitive hearing and may detect a broad range of frequencies. Relying on hearing can be particularly adaptive in the marine environment where light and other cues are often limited and natural sounds are frequently abundant. Yet these sensitive auditory abilities may also be easily impacted by anthropogenic noise. Human use of the Earth’s oceans has steadily increased over the last century, resulting in an increase in anthropogenically produced noise (e.g., National Academy of Sciences 2003 ). The Arctic is no exception to this increase (Blackwell and Greene Jr 2003 ). Reductions in polar sea ice and the opening of the Northwest Passage presumably will open up habitats for many top predators. Yet this decrease in sea ice provides greater human access to a high-latitude environment, and such a change is poised to transform a relatively pristine environment into one saturated with human activities and associated noise. Sources are varied and include naval exercises, boundary definitions, shipping/movement along Alaska’s North Slope, seismic resources exploration, and the construction of an infrastructure needed to support it (Wang and Overland 2009 ; Titley and St. John 2010 ). These changes encompass the habitats of Delphinapterus leucas (beluga whales) and other top predators. Despite this obvious overlap of humannatural interests, there is a poor understanding of influences of these sound-associated changes. To estimate the impacts of this noise, it is crucial to evaluate the natural hearing abilities and the variation with marine mammal populations. Yet a primary challenge is that audiograms of odontocete marine mammals have most often been estimated from stranded animals or nonwild individuals (for a review, see Mooney et al. 2012 ). In many instances, these records have produced valuable data that are otherwise unavailable. For example, hearing in several stranded beaked whale species have helped define what these sound-sensitive animals hear (Finneran et al. 2009 ; Pacini et al. 2011 ). The audiogram of a stranded infant Risso’s dolphin helped redefine what the species actually detects (Nachtigall et al. 2005 ). Work with trained odontocetes provides scientific data that are likely unique to those settings and can address how animals hear or how they may be protected from anthropogenic noise (Nachtigall and Supin 2008 ). Yet, in many instances, health compromised stranded animals may not have normal auditory abilities and thus are not necessarily representative of wild populations. Furthermore, without baselines for wild individuals, it is difficult to put differences and results of nonwild individuals in a relative context. Clearly, there is value in increasing the number of animals within a species measured for hearing capabilities whenever possible. Here we describe the methods and initial results for measuring the hearing of wild D. leucas (Castellote et al. 2014 ). The goal of this study was to determine hearing sensitivity in wild Bristol Bay D. leucas during a planned collection-and-release operation. Monitoring of D. leucas has been recommended in recent years because this species is likely to be negatively impacted by climate change and because such a broadly dispersed, high-trophic feeder can serve as an effective sentinel of the ecosystem(s) in which it lives (Moore 2008 ; Moore and Huntington 2008 ; Simpkins et al. 2009 ). Because noise may impact D. leucas in a variety of ways, it is essential to determine what these animals hear.

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In view of the expected changes in the Arctic acoustic environment, expanding our knowledge of D. leucas hearing is of central importance for an appropriate conservation management framework. One of the five distinct stocks of D. leucas whales that are currently recognized in US waters, the Cook Inlet D. leucas population is endangered and efforts for its recovery to date have not been successful. The impact of anthropogenic noise has been identifi ed as a serious threat, potentially impeding recovery (NMFS 2008 ). On the contrary, the Bristol Bay D. leucas population is increasing and is considered to be a healthy population (NMFS 2008 ). The acoustic environment in Bristol Bay is different; many of the chronic anthropogenic sources typically found in the Cook Inlet D. leucas habitat are essentially absent or seasonally present at lower intensities in the Bristol Bay habitat. This suggests that Bristol Bay D. leucas are a valuable asset to evaluate baseline hearing and health measures for comparison to affected populations such as Cook Inlet D. leucas .

2 Temporary Collection of Beluga Whales and Hearing Test Methods This study was conducted in September 2012 in Bristol Bay, AK. The audiograms were measured during an overall health assessment study that required the collection and release of D. leucas . Audiograms were obtained from seven of seven belugas tested. The procedures were similar to those followed by Ferrero et al. ( 2000 )and were conducted under National Marine Fisheries Service Marine Mammal Research Permit No. 14245 and approved by the necessary Institutional Animal Care and Use Committees. The full results are published elsewhere (Castellote et al. 2014 ); here we provide a summary of the methods and preliminary results. Bristol Bay is a generally shallow, muddy-bottomed estuary system that supports a population of D. leucas. Using three 3.5-m aluminum skiffs and one soft-bodied inflatable boat, we searched for an adult beluga. When a suitable animal was spotted (Fig. 88.1 ), one of the skiffs would follow and gradually approach the whale to encourage it to swim into shallow water (