04-09 / 002GB99095B / This document is not legally binding. bioMérieux reserves the right to modify specifications without notice / BIOMERIEUX, the blue logo, API, air Ideal 3P, Bi-Box, BioBall, Bio-discs, chromID, Count-Tact, CPS, Hemoline, PolyVitex, Portagerm, PREVI, Slidex, Uriline, Vikia are used, pending and/or registered trademarks belonging to bioMérieux S.A. or one of its subsidiaries / QuickVue is a trademark belonging to Quidel Corporation / ESwab is a trademark belonging to Copan / Any other name or trademark is the property of its respective owner / bioMérieux SA RCS Lyon 673 620 399 / Photos: bioMérieux, N. Bouchut. Printed in France / THERA Conseil / RCS Lyon B 398 160 242.
Your partner for
culture media
bioMérieux S.A. 69280 Marcy l’Etoile France Tel. : 33 (0)4 78 87 20 00 Fax : 33 (0)4 78 87 20 90
www.biomerieux.com www.biomerieux-diagnostics.com
Contents 4 2 Committed to Meeting Your Challenges 15 2 Product Insight -
Range and Culture Media TM
44 2 Automated Specimen Inoculation - Previ Isola 46 2 Specimen Protocols Guidelines “From Specimen to Plate”
2
Committed to Meeting Your Challenges 4 2 Committed to Meeting Your Challenges 5 2 Expertise & Innovation 6 2 International Vision - Local Proximity 8 2 Our Quality Assurance Commitment - Making Your Daily Life Easier 10 2 Complete Product Traceability - Making Your Daily Life Easier 12 2 Environmental Protection 13 2 Shelf-life and Temperature Storage 14 2 Customer Service
3
Committed to Meeting Your Challenges
Laboratories are more than ever a pivotal role in ensuring patients receive the most appropriate healthcare rapidly given the current worldwide context. • Hospitals are faced with healthcare-associated infections that are recognized worldwide as a major public health issue. They need effective microbiology solutions to help with the prevention, intervention and surveillance in the fight against HealthcareAssociated Infections (HAI). • Management of healthcare expenditure implies having the means to ensure improved patient triage, better discerned use of antibiotics, freeing up patient beds quicker etc… • Regulatory requirements are ever present and form an integral part of patient safety. • Time to result is increasingly important for many diseases; this means having a linear workflow right from sample management and colony isolation to result print out.
4
As a key part of this healthcare picture, you as a laboratory need to have confidence in the microbiology solutions you chose to address these daily challenges. This is why we are committed to offering you an extensive range of cost-effective, nutrient pre-plated media. They are technically sophisticated to help ensure you obtain easy-to-read, rapid results, whatever the quantity and type of microorganism in the specimen.
bioMérieux Expertise & Innovation With over 45 years experience in the field of microbiology, bioMérieux has unique know-how and expertise in microbial culture, enumeration, detection, identification and antimicrobial resistance testing. Rapidity of diagnosis and accuracy of treatment impact clinical outcome. This is why bioMérieux proposes an extensive range of quality media to ensure you have reliable, cost-effective microbiological solutions to empower clinical decisions. With your specific daily challenges in mind, we used our recognized expertise to create an innovative range of chromogenic media, chromID™, to provide you with the simultaneous culture and identification of microorganisms you need. Part of this exclusive range covers the screening of Multi-Drug Resistant Organisms to help in the fight against HAI. To help optimize your daily workflow, we now offer an automated plate inoculation system, PREVI™ Isola. This instrument standardizes plate inoculation and results while maximizing bacteria colony isolation.
bioMérieux is still committed to offering timely, relevant solutions now and in the future. Today, with bioMérieux, obtaining quality media solutions is simpler than ever before.
5
USA PORTLAND
International Vision Local Proximity Our International vision ensures we can anticipate your media requirements while providing specific local solutions where needed. We offer worldwide media coverage with just the right amount of proximity through our strategically placed production sites. We are closer to our customers than ever before to ensure we meet your daily needs. There are 3 European production sites (France, the UK and Spain) - the French site being the largest site in Europe - and 5 other sites spread around the world in Australia, Brazil, the U.S.A, and Canada. bioMérieux has manufactured culture media since 1963 and our main, referential site in France offers its vast industrial expertise and know-how to the other production sites. This ensures we have standardized, innovative products worldwide while being able to address more local and specific needs. This is the perfect balance to ensure you have just which media you need exactly when you need them.
6
CANADA TORONTO USA LOMBARD
UK BASINGSTOKE SPAIN MADRID
FRANCE CRAPONNE
BRAZIL RIO DE JANEIRO
AUSTRALIA BRISBANE
7
Our Quality Assurance Commitment Making Your Daily Life Easier Our objectives are to: • guarantee you have the highest, reproducible quality products which comply with the strictest standards • ensure we actively help you to meet your daily quality requirements. To be able to do this, bioMérieux employs a rigourous Quality Assurance policy for all its products and services. Our Quality System is certified ISO 9001 version 2000 for all its activities and NF EN ISO 13485 version 2004 for clinical activities, including culture media. By applying a Quality Assurance system, we ensure the reproducibility of product performance throughout a product’s life cycle. The main regulatory guarantee for our products is that they comply with the Directive 98/79/CE (1998/10/27) relative to medical devices for in vitro diagnostic use. As such our products must reach strict levels of security and safety to be able to be commercialized.
8
9
Complete Product Traceability Making Your Daily Life Easier As we are committed to providing our clients with high quality products which increase user and patient safety while ensuring complete product traceability, we also follow a number of other standards which are related to this main directive (see page 11).
To facilitate your regulatory requirements, we also provide a complete range of documents on-line in our Technical Library: www.biomerieux.com
v
• Package Inserts • Non-hazardous Product Statement • Declaration of Product Conformity Statement • Thermal Shock Statement • Quality Control Certificates
10
WHAT D O E S B I O M É R I E U X P R OV I D E TH E U S E R A S P R O O F O F P R O D U C T Q UALIT Y ? PA C K A G E I N S E R T S
WHAT D O E S IT COVE R ?
STAN DAR D FO LLOWE D
Clear Performance Details are given in the package
NF EN 13612 "Performance evaluation of in vitro diagnostic medical devices" (September 2002)
insert of each medium. This data comes from bioMérieux's internal studies and external performance evaluation studies (performed with routine specimens).
This standard concerns requirements relating to the, management, assessment and documentation of manufacturer performance evaluations.
QUALIT Y CONTROL C E R T I F I C AT E S
The Quality Control Certificate is available for each Lot: bioMérieux provides performance criteria and
NF EN 12322 "Culture media for microbiology / Performance criteria for culture media" (July 1999)
the collection strains used for Quality Control of culture media in the certificate.
This standard concerns performance of culture media used in microbiology labs (traceability, compatibility, reproducibility of product performance throughout the product’s life cycle and suitability.
KIT LABELING, T H E R M A L S H O C K S TAT E M E N T , PA C K A G E I N S E R T S
The Shelf-Life is determined from Stability Studies:
NF EN 13640 "Stability testing of in vitro diagnostic reagents" (August 2002)
bioMérieux indicates the expiration date on each kit label while storage conditions before first, and after first opening are indicated in the package insert. Temperature storage is also indicated on kit labeling (see page 13).
This standard concerns requirements for the stability of in vitro diagnostic reagents: gives specific requirement for real-time testing when generating stability data (e.g. transport determination of stability after opening.
Clear and Detailed Labeling Information: given on each product's label and package insert.
Guarantee of Compliance with the EC Regulations: bioMérieux gives this information on each product's label and package insert.
Clear and Easy Symbols for all Users: given on each product's label and package insert. Symbols reduce the need for multiple translation in local languages, simplify labeling, prevent the creation of different symbols conveying the same information.
NF EN 375 "Information provided by the manufacturer with in vitro diagnostic reagents for professional use" (April 2001) This standard concerns information supplied by the manufacturer of in vitro diagnostic reagents including reagents, calibrators, control material and kits. It defines the information which must be indicated on labels and instructions for use of each IVD product.
NF EN 1041 "Information supplied by the manufacturer with medical devices" (October 2008) This standard concerns the information to be provided by the manufacturer for different types of medical devices covered by the EU Directives.
NF EN 980 "Graphical symbols for use in the labelling of medical devices" (July 2008) This standard concerns the graphical symbols to be used on information provided by the manufacturer for medical devices.
ISO 15223 "Symbols to be used with medical device labels, labeling, and information to be supplied + amendments" (April 2000) This standard concerns symbols conventionally used to convey information essential for the correct and effective use, safe and effective use of medical devices.
PA C K A G I N G
Environmental Protection: bioMérieux confirms its
Directive 94/62/CE "Packaging and packaging waste" (December 1994)
environmental commitment with: recycled and recyclable cardboard, reduce waste volume and weight (see page 12).
This standard concerns the harmonization of the various national measures relative to packaging and packaging waste to avoid/reduce the effect on the environment. This is to offer maximum environmental protection and ensure the free circulation between member countries of goods following this standard.
11
Environmental Protection In addition to following the European directive 94/62/EC “Packaging and packaging waste, one of bioMérieux’s guiding Principles is "We belong to a community". It is only natural that we embrace a responsible attitude toward our environment in all aspects of our business worldwide as regards energy, water, paper, waste, emissions.
As part of our environmental actions for our media products, we still use recycled and recyclable cardboard to make our boxes but we have worked even further on reducing the volume of plated media packaging. By moving from our already innovative “fold-box”principle to an ingenious, new, patented fold-lockTM principle (once flattened the box stays flat) we further facilitate the disposal of pre-plated culture media boxes and reduce your daily waste volume and weight. Not only do we actively try to contribute to saving our environment but we help you to do the same.
12
25°C
Shelf-life and Temperature Storage 15°C
In addition to following the regulation of “Stability testing of in vitro diagnostic reagents” we also validate the “use” of 4-week storage at 15-25°C. This is to ensure we offer maximum flexibility in the daily work routine of laboratory technicians.
Temperature Storage Validation The shelf-life of bioMerieux's culture media is determined by stability study results. These studies are performed in real-time. During this stability study two kinds of thermal shocks are simultaneously applied on the media : • Simulation of a break in the cold chain : Thermal shocks are test temperature cycles to which products may be subjected during production or transportation. • 4 weeks at 15-25°C. The stability study consists of checking the conformity of appearance, pH, and bacteriological activity. This information is included in the Thermal Shock Statement which provides you with proof of our validation process.
Temperature
Thermal shocks including 4 weeks at 15-25°C
Storage temperature
Time Control conform
Control not conform
Shelf-life
2 Example of a thermal shock protocol for preplated media: Storage: 2-8°C 10-15°C for 4 weeks 18-25°C for 6 days, including 35-39°C for 8 hours 15-25°C for 4 weeks 2-8°C
13
Customer Service Our customers deserve only the very best and as such bioMérieux is committed to continuously improving the quality of products and services we deliver you.
Our Customer Service meets this challenge by offering a wide range of services: rapid responses to customers' questions and complaints, tailor-made customer product training courses, proactive and curative system maintenance and even workflow auditing. To ensure you receive first class service regardless of your geographical location, local bioMérieux technical assistants specialized in microbiology are ready to help you backed up by the expertise of the global customer service support team. This organization provides a winning combination of local proximity with worldwide, up-to-date knowledge to ensure our customers have the expertise and follow-up they require when needed.
14
Product Insight 17 2
Chromogenic Media for Simultaneous Isolation and Immediate Identification
23 2
Chromogenic Media for the Screening of Multidrug Resistant Organisms
25 2 Blood Agar: Complex, Selective and Differential Media 29 2 Specific Media: Selective Isolation for Specific Organisms 38 2 Simple Media: Isolation of Organisms 41 2 Media for Susceptibility Testing 42 2 Media for Environmental Controls: Surfaces, Air, Water For more details see technical sheets
15
An extensive range of chromogenic media for the simultaneous culture and identification of microorganisms
16
With chromIDTM bioMérieux offers you an extensive range of chromogenic media for the simultaneous culture and identification of microorganisms. Designed to meet your increasing daily challenges, these high quality media provide you with reliable results and true peace-of-mind. Our recognised expertise in culture, identification and susceptiblity testing naturally led us to pioneer the chromogenic media concept back in 1989. Since then, we have continued to create innovative chromogenic solutions to meet your everyday needs.
2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID CPS TM
chromID CPS
®
2 for the isolation, enumeration and direct identification of E. coli, Proteus and Enterococci and KESC in one single step using urine specimens
Isolation and enumeration of all urinary tract pathogens. Colonies are well isolated and easy to identify with real differentiating colours. Microbial enumeration with colourless background to optimise colony counting. Direct identification: chromID CPS contains specific substrates of the enzymatic activities to be detected. Greater Ease-of-use: • E. coli: ß-glucuronidase (ß-GUR). No indole test required. • Proteus: spontaneous colouration of colonies producing deaminase. • Enterococci: ß-glucosidase (ß-GLU). Greater Reliability: • Nutrient capacity. • Sensitivity of detection and specificity of colouration for the main organisms usually found in urine. chromID CPS • Identification of the bacterial group KESC (Klebsiella, Enterobacter, Ref. 43 541 • kit of 20 plates Serratia and Citrobacter). Ref. 43 549 • kit of 100 plates KESC => better colour intensity, due to increased ß-glucosidase activity.
C. albicans
K. pneumoniae (brownish-green colonies) and Enterococci (turquoise colonies)
chromID CPS / Columbia CNA + 5% sheep blood TM
chromID CPS Columbia CNA + 5% sheep blood
®
Chromogenic medium for the enumeration of organisms in urine specimens and the direct identification of Escherichia coli, Enterococcus, KESC and Proteeae. Selective isolation of fastidious bacteria. Determination of hemolysis.
Incubation: 24 hours E.coli ATCC 25922, Proteus mirabilis ATCC 43071 and Enterococci ATCC 29212
Incubation: 24 hours S. epidermis ATCC 14990 S. agalactiæ ATCC 12386 (x 2.5)
chromID CPS /Columbia CNA + 5% sheep blood Ref. Ref.
43 463 • kit of 10 plates 43 473 • kit of 20 plates*
* Consult your local bioMérieux representative for more product information and availability.
17
2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID Candida
chromID Candida
Incubation: 24 hours C. albicans ATCC 10231, C. glabrata, C. krusei ATCC 6250
of Candida albicans
TM
2 for the selective isolation of yeasts and the direct identification
C. albicans
C. krusei
Candida albicans colonies are coloured blue by the specific hydrolysis of a hexosaminidase chromogenic substrate (bioMérieux patent). The hydrolysis of a second substrate (pink colouration) differentiates mixed cultures and orients identification of other species colonies (bioMérieux patent). Immediate identification of C. albicans in just 24 hours* = Blue colonies Greater intensity for C. albicans colonies. • Optimum differentiation of mixed cultures • Orientation of identification towards Candida (C. tropicalis, C. lusitaniae and C. kefyr) = pink colonies • White colonies characteristic aspect = orientation towards yeasts or filamentous fungi
chromID Candida Ref. Ref.
43 631 • kit of 20 plates 43 639 • kit of 100 plates
chromID Salmonella TM
chromID Salmonella
2 for the selective isolation of all Salmonella serotypes
Incubation: 24 hours S. Typhimurium ATCC 14028
S. Enteritidis, P. vulgaris
Three chromogenic substrates optimize the selective isolation and differentiation of Salmonella: • Specific detection of the esterase enzymatic activity on a colourless background = Pale-pink to mauve colonies for Salmonella (reading between 18 to 24 hrs) • Differentiation of other bacteria (colonies of a different colour) • Inhibition of Gram-positive bacteria and yeasts The nutrient capacity, colour intensity, sensitivity of detection and specificity of colouration, ensure optimum differentiation of mixed cultures. chromID Salmonella enables the detection of serotype Typhi, Paratyphi and most Salmonella Lactose +
chromID Salmonella Ref. Ref.
18
43 621 • kit of 20 plates 43 629 • kit of 100 plates
* For more details see technical sheets
Selenite F broth Ref.
42 099 • kit of 20 tubes
Rappaport broth Ref.
42 091 • kit of 20 tubes
chromID S. aureus
chromID S. aureus
TM
2 for the the direct identification of S. aureus Direct identification of S. aureus is based on the spontaneous green colouration of glucosidase-producing colonies (patent pending). Greater Rapidity with the immediate identification of S. aureus = Green colonies (reading between 18 and 24 hours). Greater Reliability: • Excellent performance for the culture of S. aureus in terms of nutrient capacity, detection sensitivity and colouration specificity. • Optimum differentiation of mixed cultures due to the presence of a 2nd substrate. • Orientation of identification towards Staphylococci*= S. epidermidis (white colonies), S. saprophyticus (pink colonies), S. xylosus (mauve colonies). Inhibition of other bacteria (Gram + and Gram -) and yeasts.
Incubation: 24 hours S. aureus ATCC 25923 S. aureus (green colonies), S. saprophyticus (pink colonies), S. xylosus (mauve colonies)
S. epidermidis (white colonies)
Slidex® MRSA
chromID S. aureus Ref.
43 371 • kit of 20 plates
Ref.
73 117 • kit of 50 tests
chromID Strepto B TM
chromID Strepto B
2 for the screening of all S. agalactiae
Prevention of Perinatal Group B Streptococcal disease
Incubation: 24 hours S. agalactiae NCTC 8190
Original Principle • Three chromogenic substrates to optimise the identification of all gr. B Streptococcus = pale pink to red colonies which are round and pearly after 18-24 hour incubation*. • Excellent performance for the GBS prenatal screening in terms of nutrient capacity and sensitivity of detection. • Detection of all GBS strains, including non ß-hemolitic strains. • Differentiation of mixed cultures. • Selective inhibition of most bacteria not belonging to the species S. agalactiae, as well as yeasts. Greater Simplicity: Incubation in aerobic conditions.
chromID Strepto B
Todd Hewitt Broth + Antibiotics
SLIDEX Strepto Plus B
SLIDEX Strepto Plus
Ref. 43 461: kit of 20 plates
Ref. 42 116: kit of 20 tubes of 9 ml
Ref. 58 819: kit of 50 latex tests
Ref. 58 810: kit of 50 latex tests
* For more details see technical sheets
Incubation: 24 hours GBS vaginal specimen collection
Incubation: 48 hours GBS vaginal specimen collection
19
2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID O157:H7
chromID O157:H7 Incubation: 24 hours E. coli O157:H7 ATCC 43890 Citrobacter freundii ATCC 8090 E. coli ATCC 11229 Proteus mirabilis ATCC 12453
TM
2 for the selective isolation and presumptive identification of E. coli O157:H7 E. coli O157:H7
Citrobacter - Proteus Other E. coli E. coli O157:H7
chromID Vibrio
chromID O157:H7, an innovative chromogenic medium enabling easy differentiation between the O157:H7 serotype and other microorganisms: • identification of E. coli O157:H7 after only 24 hrs of incubation at 37°C • characteristic green to blue-green colour of E. coli O157:H7 colonies • more fertile than SMAC medium, and equally selective • inhibition of Gram-positive bacteria and yeasts • increase in selectivity with respect to Gram-negative bacteria by adding Cefixime Tellurite, for highly contaminated samples.
chromID O157:H7 Ref.
cefixime tellurite mixture
42 605 • 6 x 200 ml bottles
Ref.
42 606 • 6 x 4 ml vials
chromID Vibrio* TM
Incubation: 24 hours V. cholera
2 for the selective isolation of Vibrio Vibrio parahaemolyticus Incubation: 24 hours
Presumptive identification of V. cholerae is based on the blue-green colouration of beta Galactosidase-producing colonies. Differentiation of V. parahaemolyticus: Pink colonies based on arabinose assimilation.
chromID Vibrio Ref.
20
Fecal culture naturally contaminated by V. cholerae. Images from Dr Scheftel, Institut de Bactériologie, Strasbourg, France.
43 762 • kit of 20 plates
* Consult your local bioMérieux representative for more product information and availability.
chromID P. aeruginosa*
chromID P. aeruginosa
TM
2 for the direct identification of Pseudomonas aeruginosa The direct identification of P. aeruginosa is based on the specific pink to violet coloration of aminopeptidase-producing colonies due to the chromogenic substrate ß-alanyl-resorufamine (2 bioMérieux patents).
Incubation: 24 hours P. aeruginosa ATCC 27853 P. aeruginosa morphotypes in cystic fibrosis specimens.
chromID P. aeruginosa Ref.
43 462 • kit of 20 plates
Visualisation of golden-metallic coloured P. aeruginosa colonies.
* Consult your local bioMérieux representative for more product information and availability.
21
S C R E E N I N G
O F
M U L T I D R U G -
R E S I S T A N T
O R G A N I S M S
Isolate Colonies & Start Patient Isolation You need rapid and easy detection of MDRO* responsible for the most frequently found Healthcare Associated Infections. You recognise our expertise in culture, identification and susceptibility testing. We naturally designed a specific range of MDRO chromogenic media to meet your everyday needs. This range will help you: • Reduce the number of infections by avoiding: - cross transmission between patients through isolation - auto-infection of colonized patients. • Adjust the antibiotic surgical prophylaxis depending on the patient Multidrug-Resistant Organisms colonization status. • Optimize the use of isolation beds. • Control the level of antibiotics resistance. • Provide healthcare cost-effectiveness.
22
* Multidrug-Resistant Organisms
2CHROMOGENIC
M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID MRSA Incubation: 24 hours S. aureus ATCC 43300
chromID MRSA Innovative, specific chromogenic media range TM
MRSA nasal specimen collection. Image from D. Blanc, CHUV Lausanne
2 for the direct screening of methicillin-resistant S. aureus (MRSA)
in 18-24 hours*
chromID MRSA is dedicated to the surveillance culture or screening of MRSA to identify hospitalized patients requiring isolation. The screening of MRSA carriers with surveillance cultures is a key step in the fight against nosocomial infections. chromID MRSA has been developed to answer this major health concern.
MRSA positive specimen collection. Image from Dr. C. Nonhoff, Erasme Hospital, Belgium
Identification of MRSA strains is based on the spontaneous green colouration of α-glucosidase-producing colonies (patent pending) and the presence of an antibiotic, cefoxitin. Greater Rapidity: Extremely easy-to-read identification of MRSA = Green colonies after 18-24 hour incubation. Greater Reliability: • Excellent performance for the screening of MRSA in terms of nutrient capacity, detection sensitivity and colouration specificity. • The presence of cefoxitin enables the detection of MRSA strains including hetero-resistant strains. • The selective mixture inhibits most bacteria not belonging to the genus Staphylococcus, as well as yeasts.
chromID MRSA Ref. Ref.
43 451 • kit of 20 plates 43 459 • kit of 100 plates
Enrichment broth
Agglutination Tests
Heart-Brain broth Ref. 42 081 • kit of 20 tubes
Slidex® Staph Plus Ref. 73 115 • kit of 50 tests Ref. 73 116 • kit of 250 tests
Todd-Hewitt broth + antibiotics Ref. 42 116 • kit of 20 tubes
* For more details see technical sheets
Stronger green coloration of colonies when plates read upside down through the plate bottom
MRSA from a nasal swab specimen. Image provided by Sullivan & Nicolaides Pathology, Brisbane, Australia
Slidex MRSA Detection Ref. 73 117 • kit of 50 tests
23
2CHROMOGENIC
M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID ESBL
chromID ESBL
Incubation: 18-24 hours E. coli CIP 103982, K. pneumoniae ATCC 700603, P. mirabilis ATCC BAA-896
Enterobacteria (ESBL)
TM
2 for the screening of Extended-Spectrum ß-lactamase producing
Incubation: 24 hours E. coli CIP 103982
Incubation: 24 hours K. pneumoniae ATCC 700603
chromID ESBL agar (patent pending) contains: • A mixture of antibiotics, including cefpodoxime, enabling the selective growth of ESBL-producing enterobacteria. • Two chromogenic substrates and one natural substrate enabling the direct identification of the most frequently encountered ESBL-producing enterobacteria. - Escherichia coli: spontaneous coloration (pink to burgundy) of ß-glucuronidase-producing strains (ß-GUR). - Klebsiella, Enterobacter, Serratia, Citrobacter (KESC): spontaneous green, brownish-green or blue colouration of strains expressing a ß-glucosidase (ß-GLU). - Proteeae (Proteus, Providencia, Morganella): spontaneous dark brown to light brown colouration of strains expressing a deaminase.
Ref.
43 481 • kit of 20 plates
chromID VRE
chromID VRE
TM
Incubation: 48 hours E. faecium CCUG 36804, E. faecalis ATCC 51299
2 for the screening of E. faecium and E. faecalis showing acquired Vancomycin resistance (VRE)
E. faecalis VRE feces specimen collections
chromID VRE (patents pending) contains two chromogenic substrates (α-Glucosidase & ß-Galactosidase) and Vancomycin (8mg/l) which enable: • Direct identification of E. faecium and E. faecalis after 24 hour incubation. • Specific and selective isolation & detection of acquired Vancomycin-Resistant enterococci. • Characteristic colouration of colonies with: Bluish-green colour = E. faecalis - Violet colour = E. faecium The selective mixture inhibits: - enterococci with natural resistance (ie. E. casseliflavus and E. gallinarum…) - most Gram-negative and Gram-positive bacteria as well as yeasts.
chromID VRE Ref.
E. faecium VRE feces specimen collections
24
Whereas identification to the species or group level is direct, ESBL production must be confirmed by one or more additional tests.
chromID ESBL
43 002 • kit of 20 plates
Heart-Brain broth Ref.
42 081 • kit of 20 tubes
Vancomycin Bio-DiscsTM Ref. 54 912 • kit of 4 boxes of 50 x 6mm impregnated discs with Vancomycin 30µg concentration
2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Blood agar Blood agar are isolation media specially designed to facilitate the growth of fastidious microorganisms, Gram-positive bacteria and all the species encountered in clinical specimens. It contains a peptone mixture particularly adapted to the culture of fastidious microorganisms (streptococci, Listeria...). The presence of blood enables hemolysis determination, which is a basic criterion for the orientation of bacterial identification. Example of characteristic hemolyses: Streptococcus pneumoniae: a-hemolysis, with a greenish colouration around the colony. Streptococcus pyogenes: ß-hemolysis, with a clear zone around or under the colony. Blood agar can be used to subculture bacterial strains in order to obtain pure cultures.
Columbia agar + 5% sheep blood
Columbia agar + 5% sheep blood
2 Isolation of fastidious bacteria 2 Detection of hemolysis Columbia agar is an isolation medium specially designed to facilitate the growth of fastidious microorganisms. Supplemented with sheep blood, it is highly nutritious and therefore adapted to the culture of most bacterial species, regardless of their metabolism.
Incubation: 24 hours S. pneumoniæ ATCC 6305
Incubation: 24 hours S. pyogenes ATCC 19615
Columbia agar + 5% sheep blood Ref. Ref.
43 041 • kit of 20 plates 43 049 • kit of 100 plates
25
2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Columbia CNA agar + 5% sheep blood Incubation: 48 hours S. pyogenes ATCC 19615
Columbia CNA agar + 5% sheep blood Incubation: 48 hours S. aureus ATCC 25923
2 Selective isolation of fastidious bacteria 2 Detection of hemolysis Columbia CNA agar + 5 % sheep blood is a selective isolation medium which enables the growth of Gram-positive bacteria commonly encountered in clinical specimens. Most Gram-negative bacteria and Bacillus are inhibited by the nalidixic acid and colimycin in the agar.
Columbia CNA agar + 5% sheep blood Ref. Ref.
Columbia agar + 5% horse blood
Columbia agar + 5% horse blood
2 Isolation of fastidious bacteria 2 Detection of hemolysis
Incubation: 24 hours L. monocytogenes ATCC 35152
Columbia agar + 5% horse blood is an isolation medium which has been developed to facilitate the growth of fastidious microorganisms. Incubation: 48 hours S. pyogenes ATCC 19615 + S. pneumoniæ ATCC 49619 (x 2.5)
Columbia agar + 5% horse blood Ref. Ref.
26
43 071 • kit of 20 plates 43 079 • kit of 100 plates
43 050 • kit of 20 plates 43 059 • kit of 100 plates
Trypcase Soy Agar + 5% sheep blood
Trypcase Soy Agar + 5% sheep blood
2 Isolation of bacteria 2 Determination of hemolysis
Incubation: 48 hours S. pyogenes ATCC 19615 + S. pneumoniæ ATCC 63605
Trypcase soy agar + 5% sheep blood is an isolation medium which favours the growth of all the species currently found in clinical specimens.
Trypcase soy agar + 5% sheep blood Ref. Ref.
43 001 • kit of 20 plates 43 009 • kit of 100 plates
Chocolate agar + PolyViteX
Chocolate agar + PolyViteX
TM
2 Isolation of fastidious bacteria Chocolate agar + PolyViteX is an isolation medium particularly recommended for the growth of fastidious strains belonging to the genera Neisseria, Haemophilus, and Streptococcus pneumoniae encountered in clinical specimens. This medium is composed of a nutrient base enriched with factors X (hemin) and V (NAD) provided by the hemoglobin and PolyViteX. This medium can be used to subculture bacterial strains in order to obtain pure cultures.
Incubation: 48 hours N. gonorrhoeæ ATCC 43069
Incubation: 24 hours N. meningitidis ATCC 13090
Storage: shelf-life includes 4 week storage at 15°-25°C. Chocolate agar + PolyViteX Ref. Ref.
43 101 • kit of 20 plates 43 109 • kit of 100 plates Incubation: 48 hours N. gonorrhoeæ ATCC 43069, S. pneumoniæ ATCC 6305
27
2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Chocolate agar + PolyViteX VCAT3 agar Incubation: 48 hours N. meningitidis ATCC 13090
Chocolate agar + PolyViteX VCAT3 agar TM
N. gonorrhoeæ ATCC 43069
2 Selective isolation of Neisseria gonorrhoeae & Neisseria meningitidis Chocolate agar + PolyViteX VCAT3 agar is a selective medium for the isolation of Neisseria gonorrhoeae & Neisseria meningitidis from polymicrobial specimens (urogenital, oropharyngeal, CSF, blood cultures...). The medium is composed of a nutrient base enriched with factors X (hemin) and V (NAD) provided by hemoglobin and PolyViteX. The enhanced selectivity of the medium is obtained by the combination of antimicrobial and antifungal agents.
Storage: shelf-life includes 4 week storage at 15°-25°C. Chocolate agar + PolyViteX VCAT3 Ref.
Haemophilus Chocolate 2 agar Incubation: 24 hours H. influenzae ATCC 10211
43 611 • kit of 20 plates
Haemophilus Chocolate 2 agar 2 Selective isolation of Haemophilus Haemophilus Chocolate 2 agar is a selective medium for the isolation of the different Haemophilus speciesfrom polymicrobial specimens of human origin. This new formula is composed of a nutritive base enriched with factors X (hemin) and V (NAD) provided by hemoglobin and PolyViteX. Isolation of Haemophilus species collected from the respiratory or genital tracts is often difficult due to the presence of large amounts of associated flora. The selectivity of the media is obtained by the combination of antimicrobial and antifungal agents.
Haemophilus Chocolate 2 agar Ref. Ref.
28
43 681 • kit of 20 plates 43 689 • kit of 100 plates
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Prevention of Perinatal Group B Streptococcal disease 2 GRANADA media for the screening of S. agalactiae
Granada agar Incubation: 24 hours S. agalactiae ATCC 12386
The original principle - “Granada formulae are selective and differential media that enable the detection and identification of GBS in clinical specimens. There are 100% specific for GBS. They use Granadaene to differentiate GBS from other pathogenic bacteria. The methotrexate, included in the formulae, increases pigment production.” Dr Manuel de la Rosa, creator of the Granada media.
Granada agar 2 Selective agar for the screening and direct identification of S. agalactiae Granada agar Ref.
43 712 • kit of 20 plates
Incubation: 24 hours Streptococcus B (red to orange characteristic colonies) & Enterococcus faecalis
Todd-Hewitt broth + antibiotics Ref.
42 116 • Kit of 20 x 9 ml tubes
Granada Biphasic broth
Granada Biphasic broth 2 Selective broth for the screening and direct identification of S. agalactiae Granada biphasic broth enables in a single test: Direct specimen inoculation - Culture Identification Highly Economical: it provides significant time and workload savings Total Flexibility: • the tube can be used both in labs or for delocalised patient sampling and inoculation followed by immediate incubation of GBS within 18-24 hours • storage: 2-year shelf-life at room temperature • incubation takes place in an aerobic atmosphere
Granada Biphasic Broth
API® suspension MEDIUM
PSIpettes
Ref. 42
Ref. 70 640 • Kit of 100 ampoules (3 ml)
Ref. 70
722 • kit of 40 tubes
Incubation: 24 hours S. agalactiae ATCC 12386
250 • Kit of 400 units (5 ml)
29
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Sabouraud Gentamicin Chloramphenicol 2 agar
Sabouraud Gentamicin Chloramphenicol 2 agar
2 Selective isolation of yeasts and moulds Sabouraud Gentamicin Chloramphenicol 2 agar is a selective medium recommended for the isolation of yeasts and moulds from polymicrobial specimens. The new formula has increased nutrient capacity, colour intensity, sensitivity of detection and specificity of colouration for fungi. The increased level of peptones and dextrose favours the growth of fungal strains. The presence of gentamicin inhibits most Gram-negative and Gram-positive bacteria. The presence of chloramphenicol improves the selectivity for certain species which may be resistant to gentamicin (streptococci, Proteus, etc.). The pH of the agar which is slightly acidic, favours fungal growth more than bacterial growth.
Incubation: 24 hours C. albicans ATCC 10231
Sabouraud Gentamicin Chloramphenicol 2 agar Ref. Ref.
43 651 • kit of 20 plates 43 659 • kit of 100 plates
Dermatophytes agar* Ref.
43 062 • kit of 20 plates
Incubation: 72 hours A. niger ATCC 16404
Incubation: 72 hours Aspergillus flavus
Incubation: 72 hours Aspergillus nidulans
30
* Consult your local bioMérieux representative for further product information and availability.
Campylosel agar
Campylosel agar
2 Selective isolation of Campylobacter
Incubation: 48 hours Campylo jejuni ATCC 33291
Campylosel agar is a selective medium for the isolation of intestinal Campylobacter (C. jejuni and C. coli principally) from stools. The presence of sheep blood favours the growth of the target species. The nutrient capacity is enhanced by the use of reducing agents. Good selectivity is ensured by the antibiotic and antifungal agents present in the medium which inhibit the majority of bacterial and fungal contaminants. Characteristic Campylobacter colonies are small and greyish, and sometimes spread along the inoculation streaks.
Campylosel agar Ref.
43 361 • kit of 20 plates
A7 Mycoplasma agar A7 Mycoplasma agar
2 Selective culture and enumeration of urogenital mycoplasma A7 Mycoplasma agar is a selective medium for the culture of mycoplasmas, Ureaplasma spp. and Mycoplasma hominis from urogenital specimens. The Ureaplasma urealyticum species has been separated into two new species called Ureaplasma parvum and Ureaplasma urealyticum. For the use of this medium, they should both be considered as Ureaplasma spp. This medium combines a rich nutritive base containing peptones, horse serum and growth factors favouring the growth of mycoplasmas (cysteine, PoliViteXTM, arginine, urea). The appearance of the mycoplasma colonies is as follows: • Ureaplasma spp. “Sea urchin” colonies: round colonies surrounded by a brownish-black precipitate (due to oxidation of manganese sulfate). • Mycoplasma hominis “Fried egg” colonies: round colonies with a protruding center surrounded by a lighter halo. An antibiotic mixture inhibits the growth of most Gram-positive and Gram-negative bacteria contained in the specimen.
A7 Mycoplasma agar Ref.
43 003 • kit of 10 plates
Urea-Arginine LYO 2 Ref.
42 508 • kit for 25 tests
Mycoplasma hominis
INVERTED MICROSCOPE (OBJECTIVE X10)
Ureaplasma urealyticum
31
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
SMAC CT agar Incubation: 24 hours E. coli O157:H7 ATCC 43894
SMAC CT agar 2 Selective isolation of Escherichia coli O157:H7 The SMAC CT agar (Mac Conkey with Sorbitol) enables the detection and differentiation of enterohemorrhagic Escherichia coli of serotype O157:H7, responsible for gastro-intestinal infections or diseases. According to epidemiological data, these types of pathology may also be induced by the O157:H- serotype (non-motile bacteria unlike the O157:H7 serotype). The presence of sorbitol and tellurite enables the differentiation of E. coli O157:H7 which is characterized by colourless colonies with a brown centre. E. coli 0157:H7 strains might also generate colourless colonies with a brown centre. The other E. coli fermenting sorbitol give pink to red colonies. The selectivity for Gram-positive bacteria and certain Enterobacteriaceae is ensured by cefixime, bile salts, crystal violet and tellurite.
SMAC agar Ref.
BCSA agar Incubation: 48 hours B. cepacia ATCC 25416
43 391 • kit of 20 plates
BCSA agar 2 Selective isolation of Burkholderia cepacia BCSA agar (Burkholderia cepacia Selective Agar) is a medium recommended for the selective isolation and detection of the species Burkholderia cepacia complexe from clinical specimens of mainly respiratory origin (sputum and bronchoalveolar washing) in patients suffering from cystic fibrosis. The presence of a peptone base and sugars enable optimum growth of the microorganisms. Crystal violet and the antibiotics present in the medium inhibit most microbial species (inhibition of Pseudomonas species). A high volume of agar per plate enables good performance for culture at 72 hours.
BCSA agar Ref.
32
33 631 • kit of 20 plates
Cetrimide agar
Cetrimide agar
2 Selective isolation of Pseudomonas aeruginosa Cetrimide agar is a selective isolation medium for the detection of Pseudomonas aeruginosa from specimens of various origin. Cetrimide agar stimulates the production of pyocyanin and the fluorescence of P. aeruginosa. Its formula is an optimization of King’s A medium: the addition of a quaternary ammonium inhibits most other microorganisms. Characteristic colonies present a spontaneous pale green pigmentation and green fluorescence under ultraviolet light.
Incubation: 24 hours P. aeruginosa ATCC 10145 P. aeruginosa ATCC 10145, UV light
Cetrimide agar Ref.
43 565 • kit of 20 plates
D-Coccosel agar 2 Selective isolation of enterococci and group D streptococci
D-Coccosel agar Incubation: 24 hours E. faecalis ATCC 29212
D-Coccosel agar (Bile Esculin Agar) is used for the selective isolation and differentiation of enterococci and group D streptococci from polymicrobial specimens. The characteristic colonies of enterococci and group D streptococci are colourless or grey and surrounded by a black halo (hydrolysis of esculin). The selectivity of the medium for Gram-negative bacteria is provided by sodium azide. Bile inhibits certain Gram-positive bacteria other than enterococci.
D-Coccosel agar Ref.
43 151 • kit of 20 plates
33
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Legionella GVPC agar
Legionella GVPC agar
2 Selective isolation of Legionella
Incubation: 5 days Legionella pneumophila Legionella pneumophila (x 2.5)
Legionella GVPC agar has been specially developed for the isolation from respiratory specimens of most Legionella species, notably those responsible for infections: Legionella pneumophila, which is the species most frequently involved (Pontiac fever) but also L. bozemanii, L. longbeachae, L. dumoffii, L. jordanis, L. gormanii, L. anisa and L. micdadei. This selective medium also enables the enumeration of Legionella in water according to standards T90-431 and ISO 11731. The activated charcoal absorbs any toxic substances in the yeast extract and stimulates the growth of Legionella. The ACES buffer stabilizes the pH of the medium at 6.9, which is the optimal pH for growth. The characteristic colonies of Legionella are grey-blue in colour but which can become off-white with age. They have a regular pinkish edge and, when observed under a binocular microscope, have a ground glass appearance. GVPC medium provides excellent inhibition of growth of Gram-positive bacteria, most Gram-negative bacteria, yeasts and moulds, through an optimized combination of three antibiotics.
Legionella GVPC agar Ref.
43 031 • kit of 20 plates
Slidex® Legionella Kit Ref.
73 120 • kit of 50 tests (not CE marked)
Gardnerella agar Incubation: 24 hours G. vaginalis ATCC 14018
Gardnerella agar 2 Selective isolation of Gardnerella vaginalis Gardnerella agar is a selective isolation medium for the detection of Gardnerella vaginalis in genital specimens. Gardnerella vaginalis, alone or associated with certain anaerobic bacteria (Mobiluncus, Bacteroides and Prevotella), is responsible for various urogenital infections. The presence of human blood favours the growth of the species being tested for and produces ß-hemolysis around the colonies. ß-hemolysis obtained solely on human blood agar is highly indicative of G. vaginalis. The antibiotics included in the medium inhibit most Gram-negative contaminants and yeasts.
Gardnerella agar Ref. Ref.
34
43 411 • kit of 20 plates 43 039 • kit of 100 plates
Yersinia CIN agar
Yersinia CIN agar
2 Selective isolation of Yersinia Yersinia CIN agar is a selective isolation medium for the detection and differentiation of different species of Yersinia from stool specimens. The formula of the medium is that described by Schiemann (CIN medium: Cefsulodin, Irgasan, Novobiocin). The mannitol and neutral red present in the medium enable Yersinia to be differentiated by the colour of the colonies (dark pink to red colonies). The presence of cholate, deoxycholate, crystal violet, Irgasan and antibiotics inhibits the growth of Gram-positive and most Gram-negative bacteria.
Incubation: 24 hours Y. enterocolitica ATCC 9610 Incubation: 48 hours Y. enterocolitica ATCC 9610
Yersinia CIN agar Ref. Ref.
43 421 • kit of 20 plates 43 209 • kit of 100 plates
Clostridium difficile agar 2 Selective isolation of Clostridium difficile
Clostridium difficile agar Incubation: 48 hours C. difficile ATCC 9689
Clostridium difficile agar is a selective isolation medium which is specially developed for the growth of Clostridium difficile in stool specimens. Clostridium difficile is a causative agent of Pseudo-membranous colitis and more generally of antibiotic-associated diarrhea. The presence of sheep blood facilitates the growth of the species being tested for. The characteristic colonies are grey and sometimes spread along the inoculation streaks. The antimicrobial and antifungal agents in the medium inhibit most bacterial and fungal contaminants.
Clostridium difficile agar Ref.
43 431 • kit of 20 plates
35
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Pylori agar
Pylori agar Incubation: 48 hours H. pylori ATCC 43504
2 Selective isolation of Helicobacter pylori H. pylori ATCC 43504 (x 2.5)
Pylori agar is a selective isolation medium for the detection of Helicobacter pylori in gastric biopsies. Helicobacter pylori is a cause of gastritis and is associated with the development of gastric and duodenal ulcers. Helicobacter pylori is a microaerophilic bacterium whose viability is reduced on contact with air. These characteristics should be taken into account for transport and culture. The presence of horse plasma and PolyViteX enhances the growth of the target species. The antibiotics present in the medium inhibit the majority of bacterial contaminants.
Pylori agar Ref.
43 236 • kit of 10 plates
Portagerm pylori Ref.
42 041 • kit of 8 tubes
Schaedler agar + 5% sheep blood
Schaedler agar + 5% sheep blood
Incubation: 48 hours B. fragilis ATCC 25285
Schaedler agar + 5 % sheep blood is an isolation medium particularly suitable for the detection of obligate and facultative anaerobic bacteria. The presence of growth factors such as yeast extract, hemin and vitamin K3 and the addition of sheep blood, enable the growth of ever most fastidious species. The reducing agent (L-cystine) and high concentration of dextrose in the agar favour the growth of anaerobic species.
2 Isolation of anaerobic bacteria
Incubation: 72 hours Veillonella parvula ATCC 10790
Schaedler agar + 5 % sheep blood Ref. Ref. Incubation: 24 hours C. perfringens ATCC 13124
36
43 401 • kit of 20 plates 43 279 • kit of 100 plates
BCP agar 2 Isolation of common microorganisms YBCP agar (Lactose agar with Bromcresol purple) is an isolation and differentiation medium for the development of all organisms commonly found in specimens of various origin. It also differentiates lactose-fermenting organisms from non-fermenting organisms. Lactose (+) organisms produce yellow colonies by acidification of the medium. Non-fermenting organisms produce blue or colourless colonies.
BCP agar Incubation: 24 hours E. coli ATCC 25922 (yellow colonies) & P. vulgaris ATCC 6380 (colourless colonies)
BCP agar Ref.
43 021 • kit of 20 plates
Eosin-methylene blue agar 2 Isolation of Enterobacteriaceae Eosin-methylene blue agar is a selective isolation and differentiation medium for the detection of Enterobacteriaceae. It enables lactose- and/or sucrose-fermenting microorganisms to be differentiated from nonfermenting microorganisms. Eosin-methylene blue agar is used to detect enterobacteria in stools, urine or other biological specimens. Lactose (+) and/or sucrose (+) microorganisms produce dark purple colonies by acidification of the medium, which may be accompanied by a metallic sheen. Non-fermenting microorganisms produce colourless or slightly pinkish colonies. The presence of 2 stains inhibits the growth of Gram-positive bacteria.
Eosinmethylene blue agar Incubation: 24 hours E. coli ATCC 25922
Eosin-methylene blue agar Ref.
43 081 • kit of 20 plates
37
2SIMPLE
M E D I A : I S O L AT I O N O F O R G A N I S M S
Mac Conkey agar Incubation: 24 hours E. coli ATCC 25922 (pink colonies) & P. mirabilis ATCC 12453 (colourless colonies)
Mac Conkey agar 2 Selective isolation of Enterobacteriaceae and Escherichia coli MacConkey agar with crystal violet is a selective isolation and differentiation medium for the detection of Enterobacteriaceae in specimens of various origins. This medium is specially designed to detect the fermentation of lactose through a colour change to neutral red. • lactose (+) colonies: pink to red, sometimes surrounded by a halo of precipitated bile salts. • lactose (-) colonies: colourless or slightly beige. The selectivity for Gram-positive bacteria is provided by bile salts and crystal violet.
Storage: shelf-life includes 4 week storage at 15°-25°C.
MacConkey agar Ref. Ref.
Drigalski agar Incubation: 24 hours E. coli ATCC 25922 (yellow colonies) & Salmonella typhimurium ATCC 14028 (blue colonies)
43 141 • kit of 20 plates 43 149 • kit of 100 plates
Drigalski agar 2 Selective isolation of Enterobacteriaceae and other Gram-negative bacteria Drigalski agar is a selective isolation and differentiation medium used to identify Enterobacteriaceae and other Gram-negative bacteria in feces, urine or other biological specimens. Microorganisms that ferment lactose form yellow or yellowish-green colonies; the others produce blue, green, or bluish-green colonies. The presence of sodium deoxycholate and crystal violet inhibits the growth of Gram-positive.
Drigalski agar Ref.
38
43 341 • kit of 20 plates
SS agar
SS agar Incubation: 24 hours Salmonella typhimurium ATCC 14028 (colonies with black centre) & S. flexneri ATCC 12022 (pale pink colonies)
2 Selective isolation of Salmonella and Shigella SS agar is a selective isolation and differentiation medium recommended for the detection of Salmonella and Shigella species. The medium is inoculated from stool specimens, suspension of stools or an enrichment broth. The medium detects lactose-fermenting and thiosulfate-reducing (production of H2S) colonies. Lactose-fermenting microorganisms produce pink colonies, the others give colourless colonies. H2S-producing microorganisms produce colonies with a black centre. • Colonies of Salmonella are colourless to pale yellow, with or without a black centre. • Colonies of Shigella are colourless to pale pink or orange-coloured without a black centre. The inhibition of Gram-positive micro-organisms is obtained by a mixture of bile salts and stains.
SS agar Ref. Ref.
43 091 • kit of 20 plates 43 099 • kit of 100 plates
Rappaport broth Ref.
42 091 • kit of 20 tubes
Selenite F broth Ref.
42 099 • kit of 20 tubes
Hektoen agar
Hektoen agar Incubation: 24 hours Salmonella typhimurium ATCC 14028
2 Selective isolation of Salmonella and Shigella Hektoen agar is a selective isolation and differentiation medium recommended for the detection of Salmonella and Shigella species. The medium is inoculated from stool specimens, suspension of stools or an enrichment broth. Microorganisms that ferment one of the three sugars in the medium form yellow or yellowish-pink colonies; the others produce green to bluish-green colonies. H2S-producing microorganisms form colonies with a black centre. • Colonies of Salmonella are green to bluish-green, with or without a black centre. • Colonies of Shigella are green to bluish-green without a black centre. Inhibition of Gram-positive microorganisms is obtained by a mixture of bile salts and stains.
Hektoen agar Ref. Ref.
43 111 • kit of 20 plates 43 119 • kit of 100 plates
Rappaport broth Ref.
42 091 • kit of 20 tubes
S. flexneri ATCC 12022
Selenite F broth Ref.
42 099 • kit of 20 tubes
39
2SIMPLE
M E D I A : I S O L AT I O N O F O R G A N I S M S
CLED agar Incubation: 24 hours E.coli ATCC 25922 (yellow colonies) & P. mirabilis ATCC 12453 (colorless colonies)
CLED agar 2 Isolation of urinary tract micro-organisms CLED agar (Cystine Lactose Electrolyte Deficient) is especially useful for the isolation of urinary tract micro-organisms. It also enables the differentiation of lactose-fermenting and non lactose-fermenting bacteria (bromothymol blue indicator). • Lactose (+) bacteria produce pale yellow to yellow colonies by acidification of the medium. • Non-lactose fermenting bacteria produce green, blue or colourless colonies. The composition of the medium (weak electrolyte content) prevents the swarming of Proteus. The new CLED formula provides real advantages in terms of larger colony size, improvement of Lactose character reading, and better colony differentiation.
CLED agar Ref. Ref.
Mannitol Salt 2 agar Incubation: 24 hours S. aureus ATCC 25923
43 331 • kit of 20 plates 43 339 • kit of 100 plates
CLED Andrade agar * Ref.
43 072 • kit of 20 plates
Mannitol Salt 2 agar 2 Selective isolation of staphylococci Mannitol Salt 2 agar is intended for the selective isolation of staphylococci in human specimens and orientation of identification of S. aureus. The new formula has increased nutrient capacity, sensitivity of detection and specificity. Micro-organisms which ferment mannitol produce yellow colonies. This characteristic is a criterion for orienting identification of Staphylococcus aureus. The high content of sodium chloride in the medium limits the growth of certain bacteria other than Staphylococcus.
Storage: shelf-life includes 4 week storage at 15°-25°C. Mannitol Salt 2 agar Ref. Ref.
40
43 671 • kit of 20 plates 43 679 • kit of 100 plates
* Consult your local bioMérieux representative for further product information and availability.
2MEDIA
FOR SUSCEPTIBILITY TESTING
Mueller Hinton 2 agar 2 Susceptibility to antibiotics and sulfonamides Mueller Hinton 2 agar is a medium for antimicrobial susceptibility testing by disc diffusion. The composition of Mueller Hinton 2 agar enables the growth of non-fastidious bacteria (Enterobacteriaceae, non fermenting Gram-negative bacilli, staphylococci and enterococci) found in human pathology, while guaranteeing minimum interference from the constituents of the formula with the result of the antimicrobial susceptibility test. The concentration of divalent ions in the agar is adjusted to ensure a more accurate determination of the susceptibility of Pseudomonas to aminoglycosides and tetracyclines. The low concentration of thymine – thymidine (sulfonamide inhibitors) restricts the growth around the discs, enabling more accurate measurement of the zones of inhibition. The use of Mueller Hinton 2 agar is in compliance with the NCCLS and CA-SFM standards.
Mueller Hinton 2 agar Ref. Ref.
Mueller Hinton 2 agar Incubation: 24 hours E. coli ATCC 25922
Antibiotics Ampicillin 10 µg Cephalothin 30 µg Gentamicin 10 µg Nitrofurantoin 300 µg Trimethoprim / sulfamethoxazole 1.25 / 23.75 µg
43 301 • kit of 20 plates 43 309 • kit of 100 plates
Mueller Hinton 2 agar + 5% sheep blood 2 Susceptibility of pneumococci and other streptococci to antibiotics and
sulfonamides
Mueller Hinton 2 agar + 5% sheep blood is a medium for antimicrobial susceptibility testing by disc diffusion for strains requiring blood for their growth. The composition of Mueller Hinton 2 agar, supplemented with sheep blood, enables the growth of bacteria such as pneumococci and other streptococci, while guaranteeing minimum interference from the constituents of the formula with the result of the antimicrobial susceptibility test. The low concentration of thymine – thymidine (sulfonamide inhibitors) restricts the growth around the discs, enabling more accurate measurement of the zones of inhibition. The use of Mueller Hinton 2 agar is in compliance with the NCCLS and CA-SFM standards.
Mueller Hinton 2 agar + 5% sheep blood Incubation: 24 hours S. pneumoniae ATCC 49619
Antibiotics Oxacillin 1µg Erythromycin 15 µg
Mueller Hinton 2 agar + 5% sheep blood Ref. Ref.
43 321 • kit of 20 plates 43 329 • kit of 100 plates
41
2MEDIA
F O R E N V I R O N M E N TA L C O N T R O L S : S U R FA C E S , A I R , W AT E R
Bi-BoxTM and Count-Tact® plates
surface Count-Tact® range The reference in environmental microorganism detection and/or enumeration in hospitals Count-Tact plates are 55mm in diameter and have a grid scored on the base. The convex agar meniscus enables direct application to the test surface, such as walls, floors, utensils, or employees, for hygiene controls. Count-Tact plates are recommended to check critical control points in protected areas when establishing or revising microbiological surface monitoring techniques and programs in hospitals. Count-Tact plates also can be used to control air, notably using an air sampler.
Count tact range plates 55mm: with neutralizers For use in unprotected areas
®
Count-Tact applicator
Count-Tact agar .......................................................................... Ref. 43 501 • kit of 20 plates Count-Tact GTS agar without neutralizers ...................................... Ref. 43 582 • kit of 20 plates Count-Tact Sabouraud Dextrose Chloramphenicol agar .................. Ref. 43 580 • kit of 20 plates For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2°-25°C Count-Tact 3P Irradiated agar ...................................................... Ref. 43 691 • kit of 20 plates ................................................................................................ Ref. 43 699 • kit of 100 plates Count-Tact Irradiated agar .......................................................... Ref. 43 491 • kit of 20 plates ................................................................................................ Ref. 43 492 • kit of 100 plates Count-Tact Irradiated Sabouraud Dextrose Chloramphenicol agar .... Ref. 43 581 • kit of 20 plates For use in isolators resistant to disinfection by disinfectant agents (Peroxyd or PerAcetic Acid) Irradiated ISOLATOR Count - Tact 3P agar .................................... Ref. 43 597 • kit of 20 plates Equipment (for plates 55mm)
42
The Count-Tact Applicator standardizes surface collections in terms of time and pressure Count-Tact Applicator .................................................................. Ref. 96 300 The Bi-Box is a sterile box for collecting, transporting and incubating Bi-BoxTM .......................................................... Ref. 96 301 • 10 sterile cases for Count-Tact plates air IDEAL® is a aerobiocollector which guarantees effective measurement of aerobiocontamination air IDEAL® 3PTM .......................................................................... Ref. 96 303
air & environment 90mm plates
For use in unprotected areas Trypcase Soy agar ...................................................................... Ref. 43 011 • kit of 20 plates ................................................................................................ Ref. 43 019 • kit of 100 plates Sabouraud Dextrose agar ............................................................ Ref. 43 555 • kit of 20 plates Sabouraud Dextrose Chloramphenicol agar .................................. Ref. 43 596 • kit of 20 plates
Plates for environmental control
For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2°-25°C Trypcase Soy 3P Irradiated agar.................................................... Ref. 43 711 • kit of 20 plates ................................................................................................ Ref. 43 169 • kit of 100 plates Trypcase Soy Irradiated agar ........................................................ Ref. 43 131 • kit of 20 plates ................................................................................................ Ref. 43 556 • kit of 100 plates ...................................................................................... Ref. 43 557 • kit of 20 plates (140mm) Trypcase Soy Irradiated agar with neutralizers .............................. Ref. 43 562 • kit of 20 plates Sabouraud Dextrose Irradiated agar ............................................ Ref. 43 554 • kit of 20 plates Sabouraud Dextrose Chloramphenicol Irradiated agar .................. Ref. 43 595 • kit of 20 plates Equipment (for plates 90mm) The Bi-Box is a sterile box for collecting, transoporting and incubating Bi-BoxTM 90 ................................................................................ Ref. 96 301 • 10 sterile cases air IDEAL® is a aerobiocollector which guarantees effective measurement of aerobiocontamination air IDEAL® 3PTM .......................................................................... Ref. 96 302
water
air IDEAL® 3PTM
Culture media and an identification test in compliance with the ISO 11731, NF T 90-431 and NF T 90-461 standards. Legionella For detection and numeration GVPC agar * .............................................................................. Ref. 43 031 • kit of 20 plates ................................................................................................ Ref. 43 032 • kit of 100 plates For confirmation of the Legionella genus BCYE with L-cysteine .................................................................. Ref. 43 013 • kit of 10 plates BCYE without L-cysteine .............................................................. Ref. 43 023 • kit of 10 plates For identification of L.pneumophila et L.anisa SLIDEX® Legionella kit ** ............................................................ Ref. 73 120 • kit of 50 tests Others Enumeration of total viable aerobic microorganisms R2A agar.................................................................................... Ref. 43 551 • kit of 20 plates * Consult your local bioMérieux representative for further product information and availability. ** Not CE marked
43
Automated specimen inoculation
44
A True Breakthrough PREVI™ Isola is simply streaks ahead in automated specimen inoculation.
chromIDTM Candida Automated inoculation with mixed culture: C. albicans, C. tropicalis, C. krusei
It revolutionizes front-end media processing tasks by: • Maximizing bacteria colony isolation • Standardizing plate inoculation & results • Streamlining your lab workflow
chromIDTM CPS® Automated inoculation E. coli ATCC 25922
Streaking Ahead An original, revolutionary applicator using high precision technology standardizes and maximizes colony inoculation: • Maximum agar surface inoculated • Optimum, pressure-controlled contact with the agar while inoculating • Standard quantity of inoculum used every time This simple device is at the heart of PREVI™ Isola. It will change the way you think about inoculation and laboratory workflow.
45
The following eight "Specimen Protocols" are intended to be used as guidelines for the main types of specimen encountered in your laboratory. They help you to position our culture media according to specimen type with between I to V choices possible. The choices indicated are the most appropriate solution in terms of :
2 microorganisms tested 2 the examination performed 2 epidemiological data 2 cost / workflow ratio
46
Certain categories have been grouped together, particularly as far as specimen collections are concerned. For example, the "genital protocol" is for both men and women. The analysis procedure proposed should therefore be adapted to the specimen to be studied as not all the microorganisms indicated will necessarily be tested for. Teaming our simple and practical protocols with our extensive range of quality media provides you with an optimised workflow solution.
Specimen Protocol Guidelines “From Specimen to Plate” 48 2 Urine Protocol 49 2 Fecal Protocol 50 2 Genital Protocol 51 2 Suppuration Protocol 52 2 Throat Protocol 53 2 Sputum Protocol 54 2 Blood Culture Protocol 55 2 Cerebral Spinal Fluid Protocol
47
Color Gram 2
2 U R I N E P ROTOCOL Slide/coverslide
SYSTEMATIC ISOLATION E.coli/Proteus NonEnterococcus Staphylococcus Enterobacteriaceae Enterobacteriaceae
SPECIFIC ISOLATION
Gr.B Streptococcus
chromIDTM CPS®
I
UrilineTM Uriline Coli Uriline 3 Enterococcus
Yeasts
Streptococcus
Corynebact. Gardnerella
Mycobacteriaceae
Columbia CNA Ziehl Kinyoun-Gabett
II
chromID CPS Columbia CNA
III
CLED
IV
BCP
V
Mac Conkey
48
OR
EMB
Löwenstein Jensen
Columbia CNA or TSA
OR Drigalski
Columbia
Coletsos
Color Gram 2
2 F EC AL P ROTOCOL
Methylene blue Slide/coverslide
SYSTEMATIC ISOLATION Salmonella ENRICHMENT
SPECIFIC ISOLATION
Shigella
Yersinia
Campylobacter
E. coli
E. coli O157H7
Clostridium difficile
Vibrio
Hektoen
Yersinia CIN
Campylosel
BCP
SMAC CT
C. difficile
chromIDTM Vibrio
Rotavirus Adenovirus
DIRECT
Rappaport
C
chromID Salmonella
hCG 10
Hektoen
T
S
I
VIKIATM Rota-Adeno
Mac Conkey Selenite-F
II
OR Drigalski
SS
OR EMB
III
Mac Conkey
49
Color Gram 2
2 G E N I TA L P R O T O C O L
PortagermTM
Giemsa Slide/coverslide
SYSTEMATIC ISOLATION Streptococcus / Staphylococcus
Neisseria
I
Chocolate PolyVitexTM VCAT3
II
Chocolate PolyVitex
III
Gonoline Duo 2
Columbia CNA
chromIDTM S.aureus
Columbia CNA
Gardnerella
Enterobacteriaceae Non-Enterobacteriaceae
Yeasts
Gardnerella
Mac Conkey
chromID Candida
Columbia or TSA
BCP
Sab Genta Chloram 2
Mycoplasma
Chlamydiae
QuickVue® Chlamydiae Test
Mycoplasma IST 2
SPECIFIC ISOLATION
I
II
50
Anaerobes
Haemophilus
Listeria
Schaedler
Haemophilus Chocolate 2
Columbia CNA
Chocolate PolyVitex
Columbia or TSA
Group B Strepto Pregnant woman chromID Todd Hewitt Strepto B broth
Todd Hewitt broth
Granada
Granada biphasic broth
Columbia or TSA
2 S U P P U R AT I O N P R O T O C O L
PortagermTM Amies agar
SYSTEMATIC ISOLATION Staphylococcus
Streptococcus
Enterobacteriaceae Non-Enterobacteriaceae
Color Gram 2
SPECIFIC ISOLATION Anaerobes
Pasteurella
Yeasts
Corynebacteria
Chocolate PolyVitexTM
chromID Candida
Columbia CNA
Schaedler
I
chromIDTM S. aureus
Columbia CNA
Mac Conkey
Schaedler Vit. K3
OR Schaedler Neo Vanco
Columbia Sheep
II
Mannitol Salt 2 agar
OR
Sab Genta Chloram 2
Columbia CNA
III
Columbia CNA
51
2 T H R O AT P R O T O C O L
PortagermTM Amies agar
SYSTEMATIC ISOLATION
I
Streptococcus
Staphylococcus
Neisseria / Haemophilus
Columbia CNA
chromIDTM S. aureus
Chocolate PolyVitexTM
SPECIFIC ISOLATION Yeasts
Corynebacterium / Arcanobacterium
Aspergillus niger in ears
If necrotizing ulcerative gingivitis (Vincent’s tonsillitis suspicion)
chromID Candida
Columbia CNA
Sab Genta Chloram 2
Schaedler
Sab Genta Chloram 2
TSA
Haemophilus Chocolate 2
II
III
52
TSA
Mannitol Salt 2 agar
Chocolate PolyVitex VCAT3
Color Gram 2
Loeffler
Color Gram 2
2 S P UTU M P ROTOCOL
Giemsa Ziehl (Kinyoun Gabett)
SYSTEMATIC ISOLATION
I
Streptococcus / S. pneumoniae
Staphylococcus
Enterobacteriaceae Non-Enterobacteriaceae
Haemophilus
Yeasts
Columbia CNA
chromIDTM S.aureus
Mac Conkey
Haemophilus Chocolate 2
chromIDTM Candida
Chocolate PolyVitexTM VCAT3
Sab Genta Chloram 2
Mannitol Salt 2 agar
II
Mycobacteria
Löwenstein Jensen
Coletsos
SPECIFIC ISOLATION Aspergillus Anaerobes
(if immunocompromised patient)
Neisseria Branhamella
Legionella
Burkholderia cepacia
P. aeruginosa
RSV
Chocolate PolyVitex
GVPC
BCSA
chromID P. aeruginosa
RSV Direct IF
I Schaedler
PortagermTM
Sab Genta Chloram 2
OR QuickVue® RSV Test
53
2 B L O O D C U LT U R E P R O T O C O L
Blood culture bottle
Color Gram 2
SYSTEMATIC ISOLATION Enterobacteriaceae NonEnterobacteriaceae
Streptococcus / Enterococcus
Staphylococcus
Slidex® Pneumo-Kit
Columbia or TSA
SPECIFIC ISOLATION Yeasts (If yeasts observed upon direct examination)
III
chromIDTM Candida
III
Sab Genta Chloram 2
54
Brucella
TSA
Corynebacterium / Listeria
Campylobacter
Neisseria / Haemophilus
Anaerobes
Chocolate PolyVitexTM
Schaedler
Color Gram 2
2 CEREBR AL SPINAL FLUID
Methylene blue
P ROTOCOL
Slide/coverslide
SYSTEMATIC ISOLATION Neisseria / Haemophilus
Streptococcus / S. pneumoniae
Listeria
SPECIFIC ISOLATION
Staphylococcus
Enterobacteriaceae NonEnterobacteriaceae
Mycobacteria
Ziehl Kinyoun-Gabett Slidex® Meningite Kit 5
I
Chocolate PolyVitexTM
II
Chocolate PolyVitex VCAT3
Slidex Meningite Strepto B
Yeasts C. neoformans (If yeasts observed upon direct examination)
India Ink
Slidex Meningite MnB / E. coli K1
Columbia or TSA
Mac Conkey
Löwenstein Jensen
Coletsos
chromIDTM Candida
Sab Genta Chloram 2
55
Notes
................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................ ................................................................................................................................................................
56
04-09 / 002GB99095B / This document is not legally binding. bioMérieux reserves the right to modify specifications without notice / BIOMERIEUX, the blue logo, API, air Ideal 3P, Bi-Box, BioBall, Bio-discs, chromID, Count-Tact, CPS, Hemoline, PolyVitex, Portagerm, PREVI, Slidex, Uriline, Vikia are used, pending and/or registered trademarks belonging to bioMérieux S.A. or one of its subsidiaries / QuickVue is a trademark belonging to Quidel Corporation / ESwab is a trademark belonging to Copan / Any other name or trademark is the property of its respective owner / bioMérieux SA RCS Lyon 673 620 399 / Photos: bioMérieux, N. Bouchut. Printed in France / THERA Conseil / RCS Lyon B 398 160 242.
Your partner for
culture media
bioMérieux S.A. 69280 Marcy l’Etoile France Tel. : 33 (0)4 78 87 20 00 Fax : 33 (0)4 78 87 20 90
www.biomerieux.com www.biomerieux-diagnostics.com
