WEI-SHOU HU Department of Chemical Engineering and Materials Science University of Minnesota 257 Amundson Hall 421 Washington Avenue S.E. Minneapolis, MN 55455 U.S.A. http://hugroup.cems.umn.edu PERSONAL Birth date:

November 5, 1951, Taiwan

EDUCATION 1974 B.S. Agricultural Chemistry, National Taiwan University, Taiwan 1982 S.M. Biochemical Engineering, Massachusetts Institute of Technology 1983 Ph.D. Biochemical Engineering, Massachusetts Institute of Technology EMPLOYMENT 1976-77 Research Microbiologist, China Chlorella Industrial Corporation, Taiwan 1983-89 Assistant Professor, Department of Chemical Engineering and Materials Science, University of Minnesota 1989-94 Associate Professor, Department of Chemical Engineering and Materials Science, University of Minnesota 1994Professor, Department of Chemical Engineering and Materials Science, University of Minnesota 1998Distinguished McKnight University Professor, Department of Chemical Engineering and Materials Science, University of Minnesota 2003Professor, Biotechnology Institute, University of Minnesota HONORS and HONORARY APPOINTMENTS Presidential Young Investigator Award, National Science Foundation, 1986 Fellow, American Institute for Medical and Biological Engineering, 1997 Merck Award in Cell Culture Engineering, Engineering Foundation, 2002 Honorary University Professor, Tenjin University, China 1999Scientific Advisor, Key Laboratory of Bioreactor Technology, East China Science and Technology University, Shanghai, China 1999Marvin Johnson Award, Division of Biochemical Technology, American Chemical Society, 2005 Distinguished Lifetime Achievement Award, Society for In Vitro Biology, 2006 American Institute of Chemical Engineers, Food, Pharmaceutical, and Bioengineering Division Service Award, , 2008 American Institute of Chemical Engineers, Food, Pharmaceutical and Biotechnology Division (Division 15) Award, 2009 Society for Biological Engineering (SBE) Distinguished Service Award, 2009 Asia Pacific Biochemical Engineering Award, presented in APBiochem Conference, Kobe, Japan, 2009 1

PROFESSIONAL ACTIVITIES 1985-86 Scientific Advisory Committee, National Center for Biotechnology Development, People’s Republic of China 1986 Delegation Member, US/Japan Joint Biotechnology Conference, Lake Biwa, Japan 1986Associate Editor, Journal of Biotechnology and Biomedical Engineering 1986-present Organizer and Lecturer, Short Course on Cell and Tissue Reactor Engineering  Annually, University of Minnesota  Summer, 1987-1989, Ecole Polytechnique Federale de Lausanne, Switzerland  April, 1991, Garmisch-Partenkirshen, Germany March, 2001, Strasbourg, France January 2007, Human Genome Science, IIT Bombay, India Industrial offerings: Merck (annually since 2000), Biomarin (2001), Dendrion (2001), Centocor (2002, 2003,2004), Genentech (2006), Roche (2007) 1988 Chairman, 1st Engineering Foundation Conference on Cell Culture Engineering, Palm Coast, FL 1988-1991 Scientific Advisory Board, Helix Biocore, Inc. 1988Scientific Advisory Board, Genetic Engineering News 1988-1991 Scientific Advisory Board, International Biotechnology Laboratories, Inc. 1988 Invited Lecturer on Mammalian Cell Culture, Universite Louis Pasteur, Strasbourg, France 1989 Visiting Fellow, International Center of Biotechnology, Faculty of Engineering, Osaka University, Japan 1989 Co-Chairman, 2nd Engineering Foundation Conference on Cell Culture Engineering, December 3-8, Santa Barbara, CA 1989Consulting Editor, Cytotechnology 1990-91 Strategic Grant Selection Panel, National Sciences & Engineering Research Council of Canada 1991 Delegation Member, US/Japan Joint Biotechnology Conference, Hawaii 1991 Visiting Associate Professor, International Center of Biotechnology, Faculty of Engineering, Osaka University, Japan 1991 Lecturer, Minicourse on Bioseparations, Fenabio-Biolatina/91, Sao Paulo, Brazil 1991 Lecturer and U.S. Coordinator, NATO Advanced Study Institute, Workshop on Recent Advances in Industrial Application of Biotechnology, Kusandasi, Turkey 1991 Dow Corning Visiting Lecturer, Chemical Engineering Foundation, University of Sydney, Australia 1991 Delegation member, US/Korea Biotechnology Joint Biotechnology Conference, Seoul, Korea 1992 Guest Editor, Tissue Engineering Special Issue, Cytotechnology 1993 Delegation member, US/UK joint Cellular Engineering Conference, Chester, UK 1993 Guest Editor, Process Instrumentation and Control Special Issue, Cytotechnolgy 1994 Co-chairman, Engineering Foundation Conference on Cell Culture Engineering, Palm Coast, FL 1993Editorial Board, J. Fermentation and Bioengineering 1993 Short course on Bioprocessing Technology for Protein and Vaccine Production, 2

Development Center for Biotechnology Taipei, Taiwan. 1994 Short course on Process Development of Cell Culture. King Mongkut’s Institute of Technology Thonburi, Bangkok, Thailand. 1995,1998,1999 Organizer and lecturer, Short course on Quantitative Physiology and Metabolic Engineering, University of Minnesota 1995 Director, NIGMS Biotechnology Training Program at the University of Minnesota (supporting fifteen Ph.D. trainees) 1997 Chairman, Engineering Foundation Conference on Biochemical Engineering X, Kananaskis, Canada 1998Editorial Board, Biotechnology Advances 1998-2000 Editorial Board, Journal of Chinese Institute of Chemical Engineers 1998-2004 Scientific Advisory Board, Algenix Inc. 1999 Frontiers in Biochemical Engineering short course, Tianjin University 2001-2003 Vice chair (2001-2002), Chair (2003), Food Pharmaceutical and Bioengineering Division AIChE 2003 Short course on Cell Culture Reactor Enginering, University of Chile, Chile 2003Editorial Board, Advances in Biochemical Engineering/Biotechnology 2004Advisory Board, Society for Biological Engineering 2006Special Volume Editor, Cell Culture Engineering, Advance in Biochemical Engineering/Biotechnology 2006Co-director, Consortium for Chinese Hamster Ovary Cell Genomics 2007 Co-organizer, Short course on Cell Culture Reactor Engineering, Indian Institute of Technology, Bombay 2007 Stem Cell Engineering Short Course, City University Dublin, Fighting Blindness, Dublin, Ireland 2004-2008 Advisory boards, XII and XIII International Biotechnology Symposium 2005Advisory board, Society of Biological Engineering 2007Advisory board, Asia Pacific Biochemical Engineering 2009 Advisory board, European Society for Animal Cell Technology 2009 Visiting Professor, Department of Biochemical Science and Technology, National Taiwan University 2010 Visiting Professor, Department of Chemical and Biomolecular Engineering, National University of Singapore 2010 Adjunct Professor, Department of Biochemical Science and Technology, National Taiwan University INVITED LECTURES IN INTERNATIONAL CONFERENCES (1989- ) 1989-1996 Japanese Association of Animal Cell Technology Annual meetings 1990 European Society of Animal Cell Technology general meeting, Avignon, France 1989 Congress of Pacific Chemical Societies, Honolulu, HI 1990 Asian-Pacific Biochemical Engineering Conference, Kyung-Ju, Korea 1991 American Society for Microbiologists Annual Meeting, Dallas, TX 1991 Japan Fermentation Technology Society Annual Meeting, Hiroshima, Japan 1991 Japan Chemical Engineering Society Annual Meeting, Nagoya, Japan 1991 World Congress on Cell and Tissue Culture, Anaheim, CA 3

1991 1992 1993 1993 1993 1993 1994 1994 1994 1994 1995 1995 1996 1997 1997 1997 1998 1998 1998 1999 1999 1999 1999 2000 2000 2000 2001 2001 2001 2002 2002 2003 2003 2003 2003

Engineering Foundation Conference in Biochemical Engineering, Santa Barbra, CA International Biotechnology Conference, Crystal City, MD European Society of Animal Cell Technology general meeting, Wurzberg, Germany European Biotechnology Congress, Florence, Italy International Biomedical Engineering Conference, Kyoto University, Kyoto, Japan Japanese Association of Animal Cell Technology Annual Meeting, Nagoya, Japan Keystone Conference on Tissue Engineering, Taos, New Mexico Engineering Foundation Conference in Biochemical Engineering, San Diego, CA International Symposium on Bioprocess Engineering, Cuernavaca, Mexico IX Genetics of Industrial Microorganisms, Montreal, Canada Annual Meeting American Association for Advancement of Sciences, Atlanta Metabolic Engineering Short Course, CINVESTAV-IPN, Mexico City, Mexico First National Meeting, Society of Biochemical Engineering, Republic of China First conference on Tissue Engineering, Biomedical Engineering Center, Kyoto University, Kyoto, Japan Workshop of Animal Cell Culture-Quality Control and Applications, Shinchu, Taiwan, Republic of China Annual meeting, Society of In vitro Biology, Washington DC Metabolic Engineering Workshop, Osaka University, Japan 1998 Workshop on Vaccine Technology, Biotechnology Center, Singapore International Symposium on Biotechnology in Tropics, Osaka University, Japan Workshop on Tissue Engineering, US/Israel/Turkey Trilateral Chemical Engineering Conference, Technion, Israel Plenary lecture, Asia Pacific Biochemical Engineering Conference, Phukett, Thailand Keynote Lecture, Biomaterial Society Ann Meeting, Taiwan Cell Culture Engineering short course, The National University of Singapore Keynote lecture, Biotech2000, International Biotechnology Symposium, Berlin, Germany Keynote lecture, Japanese Association Animal Cell Technology Ann Meeting, Fukuoka, Japan Keynote lecture, International Symposium of Societies of Aphresis, Taipei, Taiwan Keynote lecture, International Symposium on Biomaterials and Tissue Engineering 2001, Taipei, Taiwan Keynote Lecture, Biotechica, Symposium on Regenerative Medicine, Hanover, Germany Plenary Lecture, Recent Advances in Fermentation Technology Conference, Society of Industrial Microbiology, Long Beach Keynote Lecture, Asian Society of Biomaterial General Meeting, Taipei, Taiwan Keynote Lecture, 80th Annual Meeting of Society of Biotechnology and Fermentation Technology, Osaka Japan Millennium lecture, University of Chile, Chile Special course, “Bioengineering Fundamentals,” National Tsing Hua University, Taiwan Invited Paper, EMCC-3, Chemical Engineering Conference for Collaborative Research in Eastern Mediterranean, Thessaloniki, Greece Plenary lecture, East China University of Science and Technology (ECUST), International Symposium on Bioprocess and Biomolecular Engineering (ISBBE) Shanghai, China 4

2003 2004 2004 2004 2005 2005 2005 2006 2007 2007 2007 2008 2009 2010

Plenary lecture, European Society for Animal Cell Technology, Granada, Spain Keynote lecture, Cell Culture Engineering IX, Cancún, Mexico Keynote lecturer, Tecnológico de Monterrey (Monterrey Tech), 5th Congress of Chemical Engineering, Monterrey, Mexico Keynote lecture, 12th International Biotechnology Symposium, Santiago, Chile GM-BIM Symposium, Joint symposium of American society for microbiologists society of Industrial microbiology. Metabolic Engineering Conference, Lake Tahoe. International Symposium on Regenerative Medicine, Hokkaido University, Sapporo Japan. Center of Excellence International Symposium, Nara Institute of Science and Technology, Nara, Japan. Keynote lecture, Annual Meeting, Society for In Vitro Biology, June 5, 2006 Keynote lecture, Computer Applications in Biology, Cancun, Mexico David Ollis lecture, North Carolina State University Keynote lecture, Annual meeting Canadian Society of Chemical Engineering, Edmonton Keynote lecture, International Biotechnology Symposium, Dalian, China Invited lecture, Syslogic symposium, Hamburg, Germany Keynote lecture, Stem Cell Engineering Conference, Society for Biological Engineering, Boston

GRADUATE FACULTY MEMBER APPOINTMENTS AT UNIVERSITY OF MINNESOTA Chemical Engineering Materials Science Biomedical Engineering Microbial Engineering M.D./Ph.D. program MEMBERSHIPS IN THE UNIVERSITY OF MINNESOTA University of Minnesota Stem Cell Institute MEMBERSHIPS IN PROFESSIONAL SOCIETIES American Chemical Society American Institute of Chemical Engineers American Society for Microbiology American Society for Cell Biology Society for Industrial Microbiology Society for Biological Engineers PUBLICATIONS

Books 1.

Belter, P., Cussler, E.L. and Hu, W.-S. (1988) Bioseparations: Downstream Processing for Biotechnology, John Wiley and Sons, New York, NY (textbook). 5

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Hu, W.-S. (1990) Laboratory Techniques on Microcarrier Culture. (Videotape and manual distributed by the BioProcess Technology Institute, University of Minnesota). Ozturk, S. and Hu, W.-S. Ed. (2006) Cell Culture Technology for Pharmaceutical and Cellular Therapy. Taylor and Francis, Pub. Hu, W-S., Ed (2006) Cell Culture Engineering, Advanced Biochemical Engineering/Biotechnology, Vol. 101.

Journal Articles and Book Chapters 1. 2. 3.

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Hu, W.-S., Jeng, S-P., Wang, H.H. and Lii, C.Y. (1978) The Factors on the Production of Chlorella growth factor and protein in a heterotrophic culture. Bull. Inst. Chem. Acad. Sinica. Hu, W.-S. and Demain, A.L. (1979) Regulation of antibiotic biosynthesis by utilizable carbon sources. Proc. Biochem. 14(9), 2-6. Brana, A.F., Hu, W.-S. and Demain, A.L. (1983) Involvement of aeration in carbon source regulation of cephem antibiotic biosynthesis in Streptomyces clavuligerus. Biotechnol. Lett. 5, 791-794. Hu, W.-S., Brana, A.F. and Demain, A.L. (1984) Carbon source regulation of cepham antibiotic production by resting cells of Streptomyces clavuligerus and its reversal by protein synthesis inhibitors. Enz. Microb. Technol. 6, 155-160. Hu, W.-S., Meier, J. and Wang, D.I.C. (1985) A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers. Biotechnol. Bioeng. 27, 585-595. Hu, W.-S., Meier, J. and Wang, D.I.C. (1985) Use of surface aerator to improve oxygen transfer in cell culture. Biotechnol. Bioeng. 28, 122-125. Hu, W.-S., Girard, D.J. and Wang, D.I.C. (1985) Serial propagation of mammalian cells on microcarriers. Biotechnol. Bioeng. 27, 1466-1476. Frame, K.K. and Hu, W.-S. (1985) Oxygen uptake of mammalian cells in microcarrier culture: Response to changes of glucose concentration. Biotechnol. Lett., 7, 147-152. Hu, W.-S. and Dodge, T.C. (1985) Cultivation of mammalian cells in bioreactors. Biotechnol. Prog. 1, 209-215 Dodge, T.C. and Hu, W.-S. (1986) Growth of hybridoma cells under different agitation conditions. Biotechnol. Lett. 8, 683-686 Hu, W.-S. and Wang, D.I.C. (1986) Mammalian cell culture technology: A review from an engineering perspective. In: Mammalian Cell Technology, Ed. W.G. Thilly, pp. 167-197, Butterworths Pub, Boston, MA. Himes, V.B. and Hu, W.-S. (1987) Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities. Biotechnol. Bioeng. 29, 1155-1163 Hu, W.-S., Dodge, T.C., Frame, K.K. and Himes, V.B. (1987) Effect of glucose and oxygen on the cultivation of mammalian cells. Dev. Biol. Standard. 66, 279-290 Dodge, T.C., Ji, G-Y. and Hu, W.-S. (1987) Loss of viability of hybridoma cell culture: A kinetic study. Enz. Microbial Technol. 9, 607-612 Tao, T-Y., Ji, G-Y. and Hu, W.-S. (1987) Human fibroblastic cells attach to controlled-charge and gelatin-coated microcarriers at different rates. J. Biotechnol. 6, 9-12. Tao, T-Y., Bohn, M.A., Ji, G-Y., Einsele, A. and Hu, W.-S. (1987) Kinetics of prourokinase production by human kidney cells in culture. J. Biotechnol. 6, 205-224 6

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Hu, W.-S. and Wang, D.I.C. (1987) Selection of microcarrier diameter for the cultivation of mammalian cells on microcarriers. Biotechnol. Bioeng. 30, 548-555 Tao, T-Y., Ji, G-Y. and Hu, W.-S. (1988) Serial propagation of mammalian cells on gelatincoated microcarriers. Biotechnol. Bioeng. 32, 1037-1052 Hu, W.-S., Tao, T.Y., Bohn, M.A. and Ji, G.Y. (1987) Kinetics of prourokinase production by mammalian cells. In: Modern Approaches to Animal Cell Technology, Eds. R.E. Spier and J.B. Griffiths, pp. 365-380, Butterworths P Frame, K.K. and Hu, W.-S. (1988) A model for density-dependent growth of anchoragedependent mammalian cells in culture. Biotechnol. Bioeng. 32, 1061-1066 Ng, T.K., Gonzalez, J.F. and Hu, W.-S. (1988) A laboratory course in biochemical engineering. Chem. Eng. Educ., Fall, 202-207 Hu, W.-S. (1988) Cell culture engineering. Trends Biotechnol. 6, 83-84 Smiley, A.L., Hu, W.-S., and Wang, D.I.C. (1989) Production of human immune interferon by recombinant mammalian cells cultivated on microcarriers. Biotechnol. Bioeng. 33, 1182-9 Ng, T.K. and Hu, W.-S. (1989) Adherence of emulsan-producing Acinetobacter calcoaceticus to hydrophobic liquids. Appl. Microbiol. Biotechnol. 31, 480-485 Sen, S., Srienc, F. and Hu, W.-S. (1989) Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study. Cytotechnology, 2, 85-94 Park, J.M., Hu, W.-S. and Staba, E.J. (1989) Cultivation of artemisia annua L plantlets in a bioreactor containing a single carbon and a single nitrogen source. Biotechnol. Bioeng. 34, 1209-1213. Kim, J-H. and Hu, W.-S. (1989) Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation. Cytotechnol., 2, 135-140 Cussler, E.L., Belter, P.A. and Hu, W.-S. (1989) Crystallization for bioseparations. Chemtech, 19, 376-384. Hu, W.-S. and Himes, V.B. (1989) Stoichiometric considerations of mammalian cell metabolism in bioreactors. In: Bioproducts and Bioprocesses, Eds. A. Fiechter, H. Okada, R. D. Tanner, pp. 33-45, Springer-Verlag Pub. Fredrickson, A.G. and Hu, W.-S. (1989) Conversion of nutrients into biomass and other products in bioreactors. In: Chemical Engineering Problems in Biotechnology, Ed. M. L. Shu, Vol. 1, pp. 231-264, American Institute of Chemical Engineers. Frame, K.K. and Hu, W.-S. (1990) The loss of antibody productivity in continuous culture of hybridoma cells. Biotechnol. Bioeng., 35, 469-476 Sen, S., Hu, W.-S., and Srienc, F. (1990) Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate. Enz. Microb. Technol., 12, 571-576 Frame, K.K. and Hu, W.-S. (1990) Cell volume measurement as an estimation of mammalian cell biomass. Biotechnol. Bioeng., 36,191-197. Philippidis, G.P., Schottel, J.L., and Hu, W.-S. (1990) Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells. Enz. Microb. Technol., 12, 854-859 Scholz, M. T. and Hu, W.-S. (1990) A two-compartment cell entrapment bioreactor with three different holding times for cells, high and low molecular weight compounds. Cytotechnol., 4, 127-137 7

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Seamans, T.C. and Hu, W.-S. (1990) Kinetics of growth and antibody production by a hybridoma cell line in a perfusion culture. J. Ferm. Bioeng., 70, 241-245. Hu, W.-S. and Oberg, M.G. (1990) Monitoring and control of animal cell bioreactors: Biochemical engineering considerations. In: Large Scale Mammalian Cell Culture Technology. Ed. A.S. Lubineicky, pp. 451-481, Marcel Dekker, Inc., New York, NY. Hu, W.-S., Seamans, T.C., Bian, Z., and Oberg, M. (1990) Animal cell bioreactors: A new look at the maturing technology. Proceedings, Asia-Pacific Biochemical Engineering Conference ‘90, Kyungju, Korea (April 22-25, 1990), pp.115-118. Favre, E., Peringer, P., Wolden, C., Kyung, Y. S. and Hu, W.-S. (1990) Retention of animal cells in a rotating wire-cage bioreactor: A mechanistic study. In: Trends in Animal Cell Culture Technology, Ed. H. Murakami, pp. 127-132, Kodansha Pub., Japan. Hu, W.-S., Scholz, M.T., Favre, E., and Seamans, T.C. (1990) A new look at animal cell bioreactor development. In: Production of Biologicals from Animal Cells in Culture. Eds. R.E. Spier, J.B. Griffiths, B. Meignier, pp. 370-380, Proceedings, European Society of Animal Cell Technology, annual meeting, Avignon, France (May 7-11, 1990), Butterworth-Heinemann Pub., Oxford, U.K Nyberg, S.C., Shatfort, R.A., Cerra, F.B. and Hu, W.-S. (1990) Bilirubin conjugation in a three compartment hollow fiber bioreactor. Proceedings, 12th annual international conference of the IEEE Engineering in Medicine and Biology Society, 12(1), 443-444 Philippidis, G.P., Schottel, J.L., and Hu, W.-S. (1991) Mathematical modeling and optimization of complex biocatalysis: A case study of mercuric reduction by Escherichia coli. (1991) In: Expression System and Processes for recombinant DNA products. American Chemical Society Symposium Series, 477, 35-49. Frame, K.K. and Hu, W.-S. (1991) Kinetic study of hybridoma cell growth in continuous culture: I. A model for non-producing cells. Biotechnol. Bioeng., 37, 55-64. Frame, K.K. and Hu, W.-S. (1991) Kinetic study of hybridoma cell growth in continuous culture: II. Behavior of producers and comparison to non-producers. Biotechnol. Bioeng., 38, 1020-102. Frame, K.K. and Hu, W.-S. (1991) Comparison of growth kinetics of producing and nonproducing hybridoma cells in batch culture. Enz. Microbial Technol., 13, 690-696. Philippidis, G.P., Malmberg, L-H., Schottel, J.L., and Hu, W.-S., (1991) Effect of gene amplification on mercuric ion reduction activity of Escherichia coli. Appl. Environ. Microbiol., 57, 3558-3564. Philippidis, G.P., Schottel, J.L., and Hu, W.-S. (1991) A model for mercuric ion reduction in recombinant Escherichia coli. Biotechnol. Bioeng., 37, 47-54. Gonzalez, J.F. and Hu, W.-S. (1991) Effect of glutamate on the degradation of pentachlorophenol by Flavobacterium sp. Appl. Microbiol. Biotechnol., 35, 100-104. Goetghebeur, S., and Hu, W.-S. (1991) Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture. Appl. Microbiol. Biotechnol., 34, 735-741. Nikolai, T.J., Peshwa, M.V., Goetghebeur, S. and Hu, W.-S. (1991) Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining. Cytotechnology, 5, 141-146. Hu, W.-S. and Peshwa, M.V. (1991) Animal cell bioreactor—Recent advances and challenges to scale-up. Can. J. Chem. Eng. 69, 409-420. 8

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Perusich, C.M., Goetghebeur, S. and Hu, W.-S. (1991) Virus production in microsphere-induced aggregate culture of animal cells. Biotechnol. Techniques, 5, 145-148. Perusich, C.M., Goetghebeur, S. and Hu, W.-S. (1991) Cultivation of mammalian cells as aggregates using microspheres. Biotechnol. Int’l., 1, 179-181. Huang, L-C. and Hu, W.-S. (1991) Recent development of animal cell bioreactors. Bioindustry (Chinese), 1, 109-114. Seamans, T.C. and Hu, W.-S. (1991) A cartridge oxygenator for mammalian cell culture in a stirred tank bioreactor. Biotechnol. Techiques, 5, 83-84. Malmberg, L-H. and Hu, W.-S., (1991) Kinetic analysis of Cephalosporin biosynthesis in Streptomyces clavuligerus. Biotechnol. Bioeng., 38, 941-947. Shatford, R.A., Nyberg, S.L., Payne, W.D., Hu, W.-S., Cerra, F.B. (1991) A hepatocyte bioreactor as a potential bioartificial liver: demonstration of prolonged tissue specific functions, Surgical Forum, 42, 54-56. Nyberg, S.L., Santilli, S., Shatford, R.A., Payne, Cerra, F.B., Knighton, D., Hu, W.-S., (1991) Microelectrode measurements of oxygen tension in collagen-hepatocyte gels. Biotechnol. Techniques, 5, 449-452. Shatford, R.A., Nyberg, S.L., Meier, S.J., White, J.G., Payne, W.D., Hu, W.-S., and Cerra, F.B., (1991) Hepatocyte Function in a hollow fiber bioreactor: a potential bioartificial liver. Journal of Surgical Research, 53, 549-557. Goetghebeur, S., and Hu, W.-S. (1991) Microsphere-induced aggregate culture of animal cells. In: Production of Biologicals from Animal Cells in Culture. Eds. R.E. Spier, J.B. Griffiths, B. Meignier, pp. 423-428, Proceedings, European Society of Animal Cell Technology, annual meeting, Avignon, France (May 7-11, 1990), Butterworth-Heinemann Pub., Oxford, U.K. Hu, W.-S., Nyberg, S.L., Shatford, R.A., Payne, W.D., and Cerra, R.B., (1991) Cultivation of hepatocytes in a new entrapment bioreactor: a potential bioartificial liver, In: Animal Cell Culture and Production of Biologicals), eds: Sasaki R., Ikura K., Kluver Academic Publishers, Dordrecht. The Netherlands; 75-80 Hu, W.-S., Vits, H., Perusich, C.M. and Gryte, D. (1991) Continuous animal cell bioreactor with cell retention. 1991 Biotechnology Conference, Sep. 12-14, 1991, Korea Advanced Institute of Science and Technology, Seoul, Korea Hu, W.-S., Kyung, Y.S., Peshwa, M.V., Perusich, C.M., and Vits, H., (1991) Dynamics and characterization of aggregate culture of mammalian cells. Proceedings, Second Korea-U.S. Bioprocessing Technology Seminar, Seoul, Korea, Dec. 12-17, 1991. Malmberg, L-H., Sherman, D.H. and Hu, W.-S., (1992) Analysis of rate limiting reactions in cephalosporin biosynthesis. Biochemical Engineering VII, Proceedings, Engineering Foundation Conference on Biochemical Engineering, March 3-8, 1991, Santa Barbara, CA, Ann. N.Y. Acad. Sci., 665, 16-27. Nikolai, T.J. and Hu, W.-S. (1992) Cultivation of mammalian cells on macroporous microcarriers. Enzyme Microb. Technol., 14, 203-208. Chao, S.H., Peshwa, M.P., Sutherland, D.E.R. and Hu, W.-S., (1992) Entrapment of cultured pancreas islets in three dimensional collagen matrices. Cell Transplant, 1, 61-66. Hu, W.-S. and Piret, J.M. (1992) Mammalian cell culture processes. Curr. Opinion Biotechnol., 3, 110-114. 9

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Huang, L-C., Vits, H., Staba, E.J., Cooke, T.J., and Hu, W.-S. (1992) Effect of embryo size on oxygen uptake rate in developing somatic embryos of Daucus carota L., Biotech. Letters, 14, 701-706. Vits, H., Hu, W.-S., Staba, E.J. and Cooke, T.J. (1992) Formulation of a morphogenetic model for embryo development in dicotyledonous plants. J. Theor. Biol., 157, 221-241. Nyberg, S.L., Shatford, R.A., Payne, W., Hu, W.-S., Cerra, F.B. (1992) Hepatocyte culture systems for artificial liver support: implications for critical care medicine (bioartificial liver support). Critical Care Medicine, 20, 1157-1168. Vits, H. and Hu, W.-S. (1992) Fluctuations in continuous mammalian cell bioreactors with retention. Biotechnol. Progr., 8, 397-403. Nyberg, S.L., Platt, J., Shirabe, K., Payne, W.D., Hu, W.-S., and Cerra, F.B., (1992) Immunoprotection of xenocytes in a hollow fiber bioartificial liver, ASAIO J. M463-M467. Konstantinov, K.B., Pambayun, R., Matanguihan, R., Yoshida, T., Perusich, C. Hu, W.-S. (1992) On-line monitoring of Hybridoma cell growth using a laser turbidity sensor. Biotechnol. Bioeng., 40, 1337-1342. Lim, H-S., Han, B-K., Kim, J-H., Peshwa, M.V. and Hu, W.-S., (1992) Spatial distribution of mammalian cells grown on macroporous microcarriers with improved attachment kinetics, Biotechnol. Progr., 8, 486-493. Malmberg, L-H. and Hu, W.-S. (1992) Identification of rate-limiting steps in cephalosporin C biosynthesis in Cephalosporium acremonium: a theoretical analysis. Appl. Microb. Biotechnol, 38, 122-128. Nyberg, S.L., Shatford, R.A., Payne, W.D., Hu, W.-S. and Cerra, F.B., (1992) Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: an in vitro system with application to the bioartificial liver. Cytotechnology, 8, 205-216. Hu, W.-S. (1992) Engineering perspectives in tissue engineering, Cytotechnology, 10, 187-188. Nyberg, S.L., Shatford, R., Peshwa, M.V., White, J.G., Cerra, F.B., and Hu, W.-S., (1993) Evaluation of a hepatocyte entrapment hollow fiber bioreactor: a potential bioartificial liver. Biotechnol. Bioeng., 41, 194-203. Hu, W.-S., (1992) Bioreactors for animal cell cultivation. In: Recent Advances in Industrial Applications of Biotechnology, Ed. F. Vardar-Sukan and S. S. Sukan, Kluwr Pub. 243-261 Vits, H., Peshwa, M.V., and Hu, W.-S., (1992) Oscillatory behavior in continuous aggregate cultures of mammalian cells, In: Animal Cell Technology: Basic & Applied Aspects, Ed. H. Murakami, S. Shirahata and H. Tachibana, Kluwer Pub. pp. 121-127, Proceedings, Japanese Association of Animal Cell Culture Technology, annual meeting, Fukuoka, Japan Kyung, Y.-S., Peshwa, M.V., Perusich, C.M., and Hu, W.-S.,(1992) Dynamics of aggregate culture of mammalian cells. In: Animal Cell Technology: Basic & Applied Aspects, Ed. H. Murakami, S. Shirahata and H. Tachibana, Kluwer Pub. pp. 65-69, Proceedings, Japanese Association of Animal Cell Culture Technology, annual meeting, Fukuoka, Japan Gryte, D.M., Peshwa, M.V., Hu, W.-S., and Ward, M.D., (1992) Detection of mammalian cell attachment and lysis on a metal surface using a piezoelectric sensor, In: Animal Cell Technology: Basic & Applied Aspects, Ed. H. Murakami, S. Shirahata and H. Tachibana, Kluwer Pub. pp. 129-134, Proceedings, Japanese Association of Animal Cell Culture Technology, annual meeting, Fukuoka, Japan. 10

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Abu-Absi, S. F., Friend, J. R., Hansen, L. K., and Hu, W.-S. (2001) Polarity and bile canaliculilike channels in self-assembled rat hepatocyte spheroids. In Proceedings of JAACT 2000. S. Shirahata, Y. Katakura, and K. Teruya Editors. Kluwer Academic Publishers, Dordrecht, The Netherlands. Abu-Absi, S.F., Hess, D.J., Groehler, K.E., Remmel, R.P., Sielaff, T.D., and Hu, W.-S. (2001) Monitoring metabolic activity and differentiated function in a bioartificial liver device. In: Methods of Tissue Engineering, pp.951-964, Eds. A. Atala and R. P. Lanza, Academic Press, San Diego, CA. Seow, T.K., Korke, R., Liang, M.E., Ong, S. E., Ou, K, Wong, K., Hu, W-S. and Chung, M. C. (2001) Proteomic investigation of metabolic shift in mammalian cell culture. Biotechnol. Prog., 17, 1137-1144 Korke, R., Rink, A., Seow, T.K., Chung, M., Beattie, C.W., and Hu, W.-S. (2002) Genomic and Proteomic Perspectives in Cell Culture Engineering. J. Biotechnol., 94, 73-92. Ribnicky, D. M., Cohen, J.D., Hu, W.-S and Cooke, T.J. (2002) An auxin surge following fertilization: a mechanism for regulating plant totipotency. Planta, 214, 505-509. Abu-Absi, S.F., Friend, J.R., Hansen, L.K., and Hu, W.-S. (2002) Structural Polarity and Functional Bile Canaliculi in Rat Hepatocyte Spheroids. Exp. Cell Res. 274, 56-67. Wong, K., Guardia, M.J., Lee, S., and Hu, W.-S. (2002) Adenovirus vector production in 293 fed-batch cultures. Proceed. 16th Eur. Soc. Animal Cell Technol. Gen. Mtg., Lugano, Switzerland, pp. 55-57. Tzanakakis, E. S., Waxman, D. J., Hansen, L. K., Remmel, R. P., and Hu, W.-S. (2002) Longterm enhancement of cytochrome p450 2b1/2 expression in rat hepatocyte spheroids through adenovirus-mediated gene transfer. Cell Biol. and Toxicol., 18, 13-27. Gerdtzen, Z.P., Daoutidis, P., Hu, W.-H. (2002) Nonlinear model reduction of energy metabolism in Saccharomyces cerevisiae. In: Modeling and Control of Biological Systems, Proceedings of the American Control Conference, May 8-10, 2002, Anchorage, Alaska , pp. 2867-2872. Schwartz, R.E., Reyes, M., Koodie, L., Jiang, Y., Blackstad, M., Johnson, S., Lund, T., Lenvik, T., Hu, W.-S., Verfaillie, C.M. (2002) Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J. Clin Invest., 96, 1291-1302. Gadgil, C., Rink, A., Beattie, C., Hu, W.-S. (2002) A mathematical model for suppression subtractive hybridization. Comp Func Genom., 3, 405-422. Narayanan, R.A., Rink, A., Beattie, C.W. and Hu, W.-S. (2002) Differential gene expression analysis during porcine hepatocyte spheroid formation. Mamm. Genom., 13, 515-523. Namjoshi, A. A., Hu, W.-S., and Ramkrishna, D. (2003) Unveiling steady-state multiplicity in hybridoma cultures: the cybernetic approach. Biotechnol. Bioeng., 81, 80-91. Gambhir, A., Korke, R., Lee, J., Fu, P-C., Europa, A.F. and Hu, W-S. (2003) Analysis of cellular metabolism of hybridoma cells at distinct physiological states. J. Biosci. Bioeng., 95, 317-327. Lee, Y.Y., Yap. M, Hu, W-S., Wong, K. (2003) Low-Glutamine fed-batch cultures of 298-HEK serum-free suspension cells for adenovirus production. Biotechnol. Prog., 19, 501-509. Korke, R., De Leon Gatti, M., Lau, A.L.Y., Lim, J.W.E., Seow, T.K., Chung, M.C.M., Hu, W-S. (2004) Large scale gene expression profiling of metabolic shift of mammalian cells in culture. J. Biotechnol., 107, 1-17. 18

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Gerdtzen, Z.P., Daoutidis, P., Hu, W.-S. (2004) Nonlinear reduction for kinetic models of metabolic reaction networks. Metabolic Eng., 6,140-154. Gadgil, C., Yeckel, A. Derby, J., Hu, W-S. (2004) A diffusion-reaction model for DNA microarray assays. J. Biotechnol., 114, 31-45. Mehra, S., Hu, W. S., Karypis, G. (2004) A Boolean algorithm for reconstructing the structure of regulatory networks. Metabolic Eng., 6, 326-39. Abu-Absi, S., Hansen, L., Hu, W.-S. (2004) Three-dimensional co-culture of hepatocytes and stellate cells. Cytotechnology, 45:125-140. Abu-Absi, S., Narayanan, R.A., Hu, W-S, (2004) Bioengineering challenges in tissue engineering. In: Advances in Biomaterials and Drug Delivery Systems, pp. 11-20, Eds: G-H., Hsiue, G-H., et al., Princeton Press. Abu-Absi, S., Narayanan, R.A., and Hu, W-S, (2004) Hepatic tissue engineering: applications in disease treatment and liver support. In: Frontiers in Biomedical Engineering, Eds: Ned, H.C., Hwang and Savio L.Y., Woo, New York, NY De Leon Gatti, S., Wlaschin, K., Rink, A., Sanny, A., Tan, K.S., Nissom, P.M., Ong, P.F., Wong, K., Philip, R.J., Cham, B., Wong, C.F., Lim, K.M., Yap, M., Hu, W.-S., (2004) Genomic exploration on Chinese hamster ovary cells. In: Animal Cell Technology Meets Genomics, Eds: F., Godia and M., Fussenegger Dordrecht, The Netherlands. 23-29. Adams, D.N., Kao, E.Y.C., Hypolite, C., Distefano, M.D., Hu, W-S., Letourneau, P.C. (2005) Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. J. Neurobiol., 62, 134-147. Lai, P.H., Sielaff, T.D., Hu, W.-S, (2005) Sustaining a bioartificial liver under hypothermic conditions. Tissue Eng., 11, 427-438. Seth, G., Philp, R. J., Denoya, C. D., Stutzman-Engwall, K.J., Yap, M., Hu, W.-S. (2005) Large scale gene expression analysis of cholesterol dependence in NS0 cells. Biotechnol. Bioeng. 90, 552-567. Abu-Absi, S., Seth, G., Narayanan R.A., Groehler, K., Lai, P., Anderson, M., Sielaff, T., Hu, W.S., (2005) Characterization of a hollow fiber bioartificial liver device. Artificial Organs. 29, 41922. Abu-Absi, S., Hansen, L., Hu, W.-S. (2005) Dexamethasone effects on rat hepatocyte spheroid formation and Function. Tiss. Eng. 11, 415-426. Gadgil, M., Kapur, V. and Hu, W-S. (2005) Transcriptional response of Escherichia Coli To temperature shift. Biotechnol. Prog. 21, 689-699. Gadgil, M., Lian, W., Gadgil, C., Kapur, V. and Hu, W-S. (2005) An analysis of the use of genomic DNA as a universal reference in two channel DNA microarrays. BMC Genomics, 6, 66. Ulloa, F, Verfaillie, C. and Hu, W.-S. (2005) Culture systems for pluripotent stem cells. J. Biosci. Bioeng., 100, 12-27. Wlaschin, K., Nissom, P.M., de Leon Gatti, M., Fern, Peh. F.O, Arleen, S., Tan, K.S., Rink, A., Cham, B., Wong, K., Yap, M., Hu, W.-S. (2005) EST sequencing for gene discovery in Chinese hamster ovary cells. Biotechnol. Bioeng. 91, 592-606. Mehra, S. and Hu, W-S. (2005) A kinetic model of quantitative real time polymerase chain reaction. Biotechnol. Bioeng. 91,848-860. Lai, P.H., Meng, Q. Sielaff, T.D., Hu, W.-S. (2005) Hypothermic maintenance of hepatocyte spheroids. Cell Transplantat. 14, 375-389. 19

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Mehra, S., Wei, L., Jayapal, K., Charaniya, S., Sherman, D. H. and Hu, W-S. (2005) A framework to analyze multiple time series data –a case study with streptomyces coelicolor. J. Ind. Microbiol. Biotechnol., 11, 159-172. Ulloa, F, Seth, G., Verfaillie, C. and Hu, W.-S. (2005) Stem cell culture engineering. J. Chinese Inst. Engineers. 28, 1039-1052. Gerdtzen, Z. P., Daoutidis, P. & Hu, W.-S. (2005) Nonlinear model reduction for metabolic networks with multiple time scales. In: Proceedings of 13th the Mediterranean Conference on Control and Automation, Limassol, Cyprus, June 27-29, 2005. Schwartz, R.E., Linehan, J.L., Painschab, M.S., Hu, W-S., Verfaillie, C.M., and Kaufman, D.S. (2006) Defined conditions for development of functional hepatic cells from human embryonic stem cells. Stem Cells Dev., 14, 643-655. Seth, G., McIvor, R.S., and Hu, W.-S. (2006) 17β-hydroxysteroid dehydrogenase type 7 (Hsd17b7) reverts cholesterol auxotrophy in NS0 cells. J. Biotechnol. 121, 241-252. Seth, G., Otzurk, M., and Hu, W.-S. (2006) Reverting cholesterol auxotrophy of NS0 cells by altering epigenetic gene silencing. Biotechnol. Bioeng., 93, 820-827. Gadgil M, Mehra S, Kapur V, Hu W-S. (2006) TimeView: For Comparative Gene Expression Analysis. Appl Bioinformatics. 5, 41-44. Cameron C.M., Hu, W-S, Kaufman D.S. (2006) Improved development of human embryonic stem cell-derived embryoid bodies by stirred vessel cultivation. Biotechnol. Bioeng. 94, 938-948. Wlaschin, K.F. and Hu, W-S., (2006) Fedbatch Culture and Dynamic Nutrient Feeding. Adv. Biochem. Eng. Biotechnol. 101, 43-74. Seth, G., Hossler, P., Yee, J. C. and Hu, W-S. (2006) Metabolic engineering in cell culture bioprocessing. Adv. Biochem. Eng. Biotechnol. 101, 119-164. Wlaschin, K. F., Seth, G. and Hu, W-S. (2006) Toward genomic cell culture engineering. Cytotechnology 50, 121-140 Hossler,P., Goh, L-T., Lee, M.M., and Hu, W-S. (2006) Glycovis: visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells, Biotechnol. Bioeng, 95, 946-960. Nissom, P.M., Sanny A., Kok, Y.J., Hiang, Y.T., Chuah, S.H., Shing, T.K., Wong, K.T., Hu, WS, Sim, M.Y, Philip, R. (2006) Transcriptome and proteome profiling to understanding the biology of high productivity CHO cells. Mol. Biotechnol, 34, 125-40. Ko, C-C., Oyen, M., Fallgatter A. M., Kim, J-H., Fricton J., Hu, W-S. (2006) Mechanical properties and cytocompatibility of biomimetic hydroxyapatite-gelatin nanocomposites. J Mater Res., 21, 3090-3098. Nahmias, Y., Schwartz R.E., Verfaillie, C.M., Odde, D.J., Hu, W-S. (2006) EndotheliumMediated Hepatocyte Recruitment in the Establishment of Liver-like Tissue In Vitro. Tissue Eng. 12, 1627-1638. De Leon Gatti, M., Wlaschin, K.F., Nissom, P.M., Yap, M., Hu, W-S. (2007) Comparative Transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment. J. Biosci. Eng. 103, 82-91 Seth, G., Philp, R. J., Lau, A., Jiun, K.Y., Yap, M. and Hu, W-S. (2007) Molecular Portrait of high productivity in recombinant NS0 cells. Biotechnol. Bioeng., 97, 933-951. Wlaschin, K., Hu W-S. (2007) Engineering Cell Metabolism for High Density Cell Culture via Manipulation of Sugar Transport. J. Biotechnol., 131,168-176. 20

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Jayapal, K.P., Lian, W., Glod, F., Sherman, D.H., and Hu, W-S. (2007) Comparative genomic hybridizations reveal absence of large Streptomyces colicolor genomic islands in Streptomyces lividans. BMC Genomics., 8, 229-241. Ulloa-Montoya, F., Kidder, B. L., Pauwelyn, K., Chase, L., Luttun, A., Crabbe, A., Sharov, A.A, Piao, Y., Ko M.S.H., Hu W.-S., Verfaillie, C.M. (2007) Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity. Genome Biol., 8(8): R163. Wlaschin, K., Hu W-S. (2007) A Scaffold for the Chinese Hamster Genome. Biotechnol. Bioeng., 98, 429-439. Charaniya, S., Mehra, S., Lian, W. Jayapal, K. P., Karypis, G. and Hu, W-S. (2007) Transcriptome dynamics based operon prediction and verification in Streptomyces Coelicolor. Nucl. Acids Res., 35,7222-7236. Griffin, T. J., Seth, G., Xie, H, Bandhakavi, S., Hu, W-S. (2007) Advancing mammalian cell culture engineering using genome-scale technologies. Trend. Biotechnol., 25, 401-408. Hossler, P., Mulukutla, B. C. and Hu, W-S. (2007) Systems analysis of N-Glycan Processing in Mammalian Cells. PlosOne, 2, e713. Jayapal, K.P., Wlaschin, K.F., Yap, M.G.S., and Hu, W-S. (2007) Recombinant protein therapeutics from CHO cells – 20 years and counting. Chem. Eng. Prog., 103(10) 40-47. Seth, G., Charaniya, S., Wlaschin, K.and Hu, W.S. (2007) In Pursuit of a Super ProducerAlternative Paths to High Producing Recombinant Mammalian Cells. Current Opinion in Biotechnol., 18, 557-564. Adams, D., Korke and Hu, W-S (2007) Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation. In: R. Pörtner, Editor, Animal Cell Biotechnology: Methods and Protocols, Second Edition (Methods in Biotechnology Series #24) Humana Press, Totowa, NJ. Jayapal, K.P., Philp, R.J., Kok, Y-J., Yap, M.G., Sherman, D.H., Griffin, T.J., and Hu, W-S. (2007). An integrated genomic and proteomic portrayal of growth-phase dependent physiology in Streptomyces coelicolor. PlosOne 3, e2097. Jayapal, K.P., Lian, W., Charaniya, S., Mehra, S., Glod, F., Kyung, Y-S., Sherman, D.H. and Hu, W-S. (2008). Genome-wide transcriptome analysis reveals that a pleiotropic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2). BMC Genomics, 9:56 Subramanian, K. and Hu, W-S. Stem cell culture engineering. (2008) In: W.C. Low and C. M. Verfaillie, Editors, Stem Cells And Regenerative Medicine, pp. 513-541. World Science Press, Singapore. Yee, J.C., de Leon Gatti, M, Philp, R.J, Yap, M., and Hu, W-S. (2008) Genomic and proteome exploration of CHO and hybridoma cells under sodium butyrate treatment. Biotechnol. Bioeng. 99, 1186-1204. Chatterjee, A., Kaznessis, Y, Hu, W-S. (2008) Tweaking Biological Switches Through a Better Understanding of Bistability Behavior. Current Opinion in Biotechnol, 19, 475-481. Charaniya, S., Hu, W-S. and Karypis, G. (2008) Mining bioprocess data: opportunities and challenges, Trend. Biotechnol., 26, 690-699. Yee, J.C., Wlaschin, K.F., Chuah, S.H., Nissom, P.M., Hu, W-S. (2008) Quality Assessment of Cross-Species Hybridization of CHO Transcriptome on a Mouse DNA Oligo Microarray. Biotechnol. Bioeng., 101 (6), 1359-1365. 21

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Cameron, C. M., Harding, F., Hu, W-S. and Kaufman, D.S. (2008) Effect of hypoxia on hematopoietic development in human embryonic stem cell-derived embryoid bodies. Exp. Biol. Med., 233, 1044-1057. Charaniya, S., Karypis, G., and Hu, W-S. (2009) Mining transcriptome data for function-trait relationship of hyper productivity of recombinant antibody. Biotechnol. Bioeng. 102, 1654-1669. Yee, J.C., Gerdtzen, Z.P., and Hu, W-S. (2009) Comparative transcriptome analysis to unveil genes affecting recombinant protein productivity in mammalian cells. Biotechnol. Bioeng., 102, 246-263. Kantardjieff, A., Nissom, P. M., Chuah, S.H., Yusufi, F., Jacob, N.M., Muluktla, B. C., Yap, M. and Hu, W-S. (2009) Developing genomic platforms for Chinese hamster ovary cells. Biotechnol. Adv., 27, 1028-1035. Jacob, Nitya M., Kantardjieff, A., Yusufi, F.; Retzel, E., Mulukutla, B.C., Chuah, S-H; Yap, M.; Hu, W-S. (2010) Reaching the depth of the Chinese hamster ovary cell transcriptome. Biotechnology and Bioengineering, 105, 1002-1009. Kantardjieff, A.; Jacob, N. M., Yee, J.C. Epstein, E., Kok, Y-J.; Philp, R., Betenbaugh, M. and Hu, W-S. (2009) Transcriptome and proteome analysis of Chinese hamster ovary cells under low temperature and butyrate treatment. J. Biotechnol., 145, 143-159. Jacob, N.M., Loo, B.L.W, Yap, M.G.S., and Hu, W-S. (2009) Using Genomic Tools to Improve the Production of Biologics. Chem. Eng. Prog., 105,35-42. Kantardjieff, A., Seth, G., McIvor, S. and Hu, W-S. (2010) Genetic Manipulations of Mammalian Cells for Protein Expression. In: Manual of Industrial Microbiology and Biotechnology, Eds: R. H. Baltz, A. L. Demain, J.E. Davis, 3rd Ed. , pp. 330-344, ASM Press, Pub. Subramanian, K., Geraerts, M., Pauwelyn, K.A., Park, Y., Owens, D. J., Juijtjens, M., UlloaMontoya, F., Jiang, Y., Verfaillie, C.M. and Hu, W-S (2010) Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow. In: Cellular Programming and Reprogramming: Methods and Protocols, Methods in Molecular Biology. Vol. 636 pp. 55-78. DOI 10.1007/978-1-60761-691-7-4. Charaniya, S., Le, H., Rangwala, H., Mills, K., Johnson, K., Karypis, G. and Hu, W-S. (2010) Mining manufacturing data for discovery of high productivity process characteristics. J. Biotechnol., 147 (2010) 186-197 Roelandt P, Pauwelyn KA, Sancho-Bru P, Subramanian K, Ordovas L, Vanuytsel K, Geraerts M, Firpo M, De Vos R, Fevery J, Nevens F, Hu WS, Verfaillie CM (2010) Human Embryonic and Rat Adult Stem Cells with Primitive Endoderm-Like Phenotype Can Be Fated to Definitive Endoderm, and Finally Hepatocyte-Like Cells. PLoS ONE 5(8): e12101. doi:10.1371/journal.pone.0012101 Yee, J. C., Jacob, N. M, Jayapal, K. P., Kok, Y-J., Philp, R. and Hu, W-S. (2010) Global assessment of protein turnover in recombinant antibody producing myeloma cells. J. Biotechnol., 148, 182-193. Mulukutla, B.C., Khan, S., Lange, A. and Hu, W-S. (2010) Glucose Metabolism of Cultured Mammalian Cells: New twists in vintage pathways. Trends Biotechnol. 28, 476-484. Deshpande, R., Sharma, S., Verfaillie, C.M., Hu, W-S., Myer, D. L. (2010) A scalable approach for discovering conserved active subnetworks across species. PLoS Comput Biol 6(12), e1001028. doi:10.1371/journal.pcbi.1001028 22

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Jayapal, K.P., Sui, S., Philip, R.J., Kok, Y-J., Yap, M., Griffin, T. and Hu, W-S. (2010) A MultiTagging Proteomic Strategy to Estimate Protein Turnover Rates in Dynamic Systems. J. Proteome Res. 9 (5), 2087-97. Park, Y. , Subramanian, K., Verfaillie, C.M and Hu, W-S. Expansion and hepatic differentiation of rat multipotent adult progenitor cells in microcarrier suspension culture. J. Biotechnol. (Accepted) Subramanian, K., Park, Y., Verfaillie, C.M and Hu, W-S. Scalable expansion of multipotent adult progenitor cells as three-dimensional cell aggregates. Biotechnol. Bioeng (Accepted) Khan, S., Wu, C., Mulukutla, B.C., Hu, W-S. and Lange, A. (2010)Profiling the hepatic transcriptome and proteome of type 2 diabetic mice treated with bisphosphatase deficient PFK-2. Ame. J. Physiol. – Endocr. Metab. (submitted) Chatterjee, A., Johnson, C., Shu, C.-C., Kaznessis, Y. N., Ramkrishna, D., Dunny, G. M., and Hu, W.-S. (2010). Transcriptional Interference and Antisense Regulation confer a Bistable switch in Enterococcus faecalis conjugation. Molecular Cell (submitted). Johnson K.C., Nissom P.M., Hackl M., Jacob N.M., Lee L.H., Yap M., Hu W-S. (2010) Conserved MicroRNA Expression in Chinese Hamster Ovary Cell Lines. Biotechnol. Bioeng. (accepted). Castro-Melchor, M., Charaniya, S., Karypis, G., Takano, T. and Hu, W-S. Genome-wide Inference of Regulatory Networks in Streptomyces coelicolor. BMC Genomics (accepted) D’Alia, D., Eggle, D., Nieselt, K., Hu., W-S., Bretling, R. and Takano, E. Deletionof the signaling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation an primary metabolism in Streptomyces coelicolor A3(2). Microbial Biotechnol. (accepted)

Book Reviews 1 2 3

Hu, W.-S. (1986) Computer Games and Simulation for Biochemical Engineering, H.R. Bungay. Quar. Rev. Biol. 61, 244. Hu, W.-S. (1988) Biotechnology. Volume 4: Microbial Products II. Eds. H.J. Rehm and G. Reed. J. Amer. Chem. Soc. 110(13), 4478-4479. Vits, H. and Hu, W.-S. (1992) Developmental systems: at the cross road of system theory, computer science and genetic engineering, S. Wegrzyn, J.-C. Gille and P. Vidal, Quar. Rev. Biol., 67, 4:526.

PATENTS 1 Giard, D.J., Hu, W.-S., Wang, D.I.C., Detachment of anchorage-dependent cells from microcarriers - by treatment with proteolytic enzyme e.g. trypsin pronase collagenase or proteinase-K . Patent Assignee: M.I.T.; #WO 8601531; Date: March 13, 1986. 2 Hu, W.-S. and Scholz, M.T., Two-compartment three-zone bioreactor for animal cell culture Assignee: Regents of University of Minnesota. European patent, Publication No. 0380610B1, March 29, 1995. 3 Clapper, D.L. and Hu, W.-S., Cell culture support containing a cell adhesion factor and a positively-charged molecule. Patent No. 5,512,474, April 30, 1996. 4 Hu, W.-S. and Goetghebeur, S., Method for aggregating cells with small microspheres, Patent No. 5,114,855, May 19, 1992. 23

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Hu, W.-S., Cerra, F.B., Nyberg, S.L., Scholz, M.T., Shatford, R.A. Bioreactor device with application as a bioartificial liver, US Patent No. 5,605,835, February 25, 1997. Japan Patent No. 3692147. Sept 7, 2005. Hu, W.-S. and Cerra, F.B. Bioreactor device employing hepatocyte spheroids. Patent No. 5,595,909, January 21, 1997. Sherman, D.H., Hu W.-S., Malmberg L-H. Method for increasing production of microbial metabolites by genetic engineering, Patent No. 5,474,912, December 12, 1995. Hu, W.-S., Europa, A. Process for Continuous Culture of Cells, U. S. and European Patent (Application No. PCT/US98/05398, 1998; approved April 2000) Hu, W-S., Lian, W., and Sherman, D.H. Enhancement of biological production of clavulanic acid and related compounds. US Patent application filed. Seth, G. and Hu, W-S. Restoration of cholesterol independence and its use as a selectable marker in ns0 cell culture. US and India Patent application filed. Subramanian, K., Verfaillie, C. and Hu, W-S. Stem cell aggregates and methods for making and using. US Patent application filed.

Graduate Students Supervised M.S. Students 1985 Timothy Dodge, Genencor International, Inc. 1987 Emily Tao, Genzyme Corporation 1988 Mark Oberg, Exxon 1988 Matthew Scholz, 3M 1988 T. Seamans, Merck 1989 Sankar Sen, Baruch College, City University of New York 1990 Thomas Nikolai, Abbott Labs 1992 Li-Chung Huang, Centocor 1992 David Gryte, Intervet, Inc. 1993 Cheryl Kussow, Eli Lilly and Company 1994 Brian McLaughlin, Lowell Hospital 1994 Jutta Altenbach-Rehm, Universitat Karlsruhe 1995 Stephan Bancel, BioMerieux 1995 Robert Schmidt, 1997 Ryan Taber, Abbott Laboratories 1998 Jodi Maas , Biovest International 1999 Jeffrey Barton, Eli Lilly 1999 Paul Hermes, Pfizer 2002 Pam Lai, University of Minnesota, Medical School 2002 Gargi Seth, Genentech 2003 Sharath Ram Vasdevan, Intel India 2003 Shen Dong, University of Minnesota 2008 Cornelia Bengea, Abbott Laboratories Ph.D. Students 1988 Terry Ng, American Home Products Corp. 1988 Kelly Frame, Frame and Spence Consulting LLP 24

1989 1990 1990 1993 1993 1993 1995 1995 1995 1996 1997 1998 1998 1999 1999 2000 2000 2000 2000 2001 2001 2001 2001 2002 2003 2004 2004 2004 2005 2005 2005 2005 2006 2006 2006 2007 2007 2007 2008 2008 2008 2009 2010 2010 2010

Vaughn Himes, Seattle Genetics George Philippidis, Applied Research Center, Florida International University Jorge Gonzalez, University Nacional de Mar del Plata Hugo Vits, Shell-India Madhusudan Peshwa, Maxcyte, Inc. Scott Nyberg, Mayo Clinic Li-Hong Malmberg, Abbott Laboratories Chung-Ming Chi, Raytheon Engineers and Constructors, Inc. Bryce Pierson, Fairview Hospital Florence Wu, InVitrogen Chun Zhang, Shire Chang-Chun Hsiao, Chang-Gung University, Taiwan Anurag Khetan, Biogen-IDEC Anna Europa, University of the Phillippines Anshu Gambhir, 3M-India Yun Seung Kyung, Centocor, Inc. Claire Hypolite, Edison High School Derek Adams, Alexion Pharmaceuticals, Inc. Julie Friend, Dupont Susan Abu-Absi, Bristol-Myers Squibb E. Manolis Tzanakakis, University of New York, Buffalo Edmund Kao Chetan Gadgil, Indian Institute of Chemical Research, Pune, India Rashmi (Korke) Kshirsager, Biogen-IDEC R.A. Narayanan, Customised Technologies Marcela de Leon Gatti, Abbot Mugdha Gadgil, Indian Institute of Chemical Research, Pune, India Robert Schwartz, Harvard Medical School Sarika Mehra, IIT – Bombay, India Jongchan Lee, Serono Ziomara Gerdtzen Hakim, University of Chile, Chile Wei Lian, Abbott Gargi Seth, Genentech Patrick Hossler, Abbott Fernando Ulloa, Glaxo Smith-Kline (Belgium) Katie Wlaschin, 3M C. M. Cameron, Merck David Umulis, Purdue University Joon Chong Yee, Genzyme Karthik Jayapal, Bayer Salim Charaniya, Genentech Anne Kantardjieff, Alexion Yonsil Park, katholic Université, Leuven, Belgium Kartik Subramanian, Abbott Marlene Castro-Melchor, Bristol Myers Squibb 25

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