Tools for

NEURAL STEM CELL RESEARCH

Reagents for Neural Stem Cell Research

B

reakthrough studies have recently rejected the long-standing belief that neuronal tissue is incapable of regeneration. The discovery that neurons, astrocytes and oligodendrocytes arise from neural stem cells located in specific regions of the central nervous system has important clinical applications for the treatment of life-threatening, neuronal diseases, including Parkinson’s Disease. The successful engraftment of neural stem cells following implantation into the brain of rodent models has demonstrated the potential of this cell type. The development of neural stem cell biology requires specific research tools that allow the isolation, expansion and characterization of this stem cell type. In addition, the availability of reagents for the characterization of progenitor cells is critical for the analysis of their therapeutic potential.

Rabbit anti-Nestin (Catalog Number AB5922) staining of U251 cells. Shown are cells stained with anti-Nestin (green) and bisbenzimide (blue). Photo courtesy of Dr. Conrad Messam, Dr. Jean Hou and Dr. Eugene Major, NINDS, NIH, Bethesda, MD. Reprinted from Experimental Neurology (2000) 161:585-596.

Growth Factors Leukemia Inhibitory Factor – An Important Neural Stem Cell Growth Factor

T

he efficacy of CHEMICON’s patented cytokine human Leukemia Inhibitory Factor (hLIF) in the expansion of neural stem cells has recently been reported1. Isolated neural stem cells were cultured in serum-free media containing hLIF, human epidermal growth factor (hEGF) and human basic fibroblast growth factor (hbFGF). Using this system, it was shown that the initial expansion rate of neural stem cells were similar to those cultured in the absence of hLIF (hEGF and hbFGF only). However, neurospheres cultured in the presence of hLIF exhibited superior doubling rates (7-10 days) after 50-60 days in vitro. Significantly, cultures maintained in hLIF consistently produced more neurons than parallel cultures grown in the absence of hLIF. In addition, LIF-treated cells remained multipotent for at least 1 year in vitro, leading to a 107 increase in the cell population. Description

Format

Quantity

Catalog Number

Brain Derived Neurotrophic Factor [BDNF], Recombinant

Pur

10 µg

GF029

Epidermal Growth Factor [EGF], Recombinant

Pur

500 µg

GF001

Epidermal Growth Factor [EGF]

Pur

100 µg

EA135

ESGRO® (LIF), Mouse

106 Units/ 1 mL

ESG1106

ESGRO® (LIF), Mouse

107 Units/ 1 mL

ESG1107

ESGRO® (LIF), Rat

106 Units/ 1 mL

ESG2206

ESGRO (LIF), Rat

10 Units/ 1 mL

ESG2207

Fibroblast Growth Factor basic [FGF-2; bFGF], Recombinant

Pur

10 µg

FA009-1

Fibroblast Growth Factor basic [FGF-2; bFGF], Recombinant

Pur

50 µg

GF003

Glial Derived Neurotrophic Factor [GDNF], Recombinant Human

Pur

10 µg

GF030

Leukemia Inhibitory Factor [LIF], Recombinant, Human

Pur

5 µg

LIF1005

Leukemia Inhibitory Factor [LIF], Recombinant, Human

Pur

10 µg

LIF1010

Leukemia Inhibitory Factor [LIF], Recombinant, Mouse

Pur

5 µg

LIF2005

®

7

Leukemia Inhibitory Factor [LIF], Recombinant, Mouse

Pur

10 µg

LIF2010

Leukemia Inhibitory Factor [LIF], Recombinant, Rat

Pur

5 µg

LIF3005

Leukemia Inhibitory Factor [LIF], Recombinant, Rat

Pur

10 µg

LIF3010

Nerve Growth Factor 2.5s [β-NGF]

Pur

50 µg (2x25µg)

NC011

Nerve Growth Factor-β [β-NGF;2.5S]

Pur

20 µg

GF028

Neurotrophin 3 [NT3]

Pur

10 µg

GF031

Neurotrophin 4/5 [NT4/5]

Pur

10 µg

GF032

Platelet Derived Growth Factor-AA [PDGF-AA], Recombinant

Pur

10 µg

GF017

Platelet Derived Growth Factor-AB [PDGF-AB], Recombinant

Pur

10 µg

GF106

Platelet Derived Growth Factor-BB [PDGF-BB], Recombinant

Pur

10 µg

GF018

1. Carpenter, M.K., et al. (1999) Experimental Neurology 158:265-278.

2

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Neural Stem Cell Differentiation STEM CELLS: GROWTH FACTORS (LIF, bFGF, EGF) Markers: Vimentin, Nestin, HSA (lo), MHC II neg

ENGRAFTMENT Markers: BrdU, Golgi Zone, Ki-67, N-CAM, Human Nuclei

DIFFERENTIATION Following Removal Of Growth Factors

NEURONS Markers: NSE, Neurofilament 68 kD, 70 kD, 200 kD, Tau, β Tubulin III, Synaptophysin, DDC, GABA, TH, NeuN, MAP 2a + 2b

OLIGODENDROCYTES

ASTROCYTES

Markers: A2B5, CNPase, Galactocerebroside C, O1, O4, anti-Oligodendrocytes, MBP, NG2

Markers: GFAP, S100

Characterization of Stem Cells and Their Differentiated Progeny

T

he CHEMICON panel of neuroscience reagents includes antibodies specific for neural stem cell markers, including Nestin and Vimentin, and for the phenotypical analysis of differentiated progeny including neurons, oligodendrocytes and astrocytes. This panel also includes reagents for the detection of engrafted human neural stem cells following implantation into immunodeficient mice.

Stem Cell Markers

N

eural stem cells are defined as cells having the ability to self-renew, and to differentiate into multiple phenotypic lineages. Undifferentiated neural stem cells express high levels of the intermediate filament proteins Vimentin and Nestin. Additionally, proliferating neural stem cells usually show little or no expression of mature cell markers.

Using cell sorting technology, it has recently been demonstrated that adult mouse neural stem cells can be purified to 80% homogeneity by using a panel of markers that includes Nestin (high), Heat Stable Antigen (low) and Peanut Agglutinin binding (low)2.

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

BCRP1 [Breast Cancer Resistance Protein; Placenta-specific ATP-binding cassette transporter; ATP-binding cassette, sub-family G, member 2 (ABCG2)], clone 5D3 (Hu, Not Ms)

FC

Ms IgG2b

Pur

100 µg

MAB4155

c-kit [CD117; SCF Receptor], clone 2B8 (Ms)

IP, FC

Rt IgG2aκ

Pur

500 µg

CBL1359

c-kit [CD117, SCF Receptor], clone YB5.B8 (Hu)

IH, IP, FC

Ms IgG1

Pur

100 µg

MAB1162

c-kit [CD117; SCF Receptor], clone KIT4 (Hu)

IP, IF, FC

Ms IgG2a

Pur

100 µg

MAB1163

C-X-C Chemokine Receptor 4 [CD184; CXCR4], N-terminus (Hu)

IB, IC, IP

Rabbit

Pur

100 µg

AB1846

C-X-C Chemokine Receptor 4 [CD184; CXCR4], Extracellular loop (Hu)

IB

Rabbit

Pur

100 µg

AB1847

C-X-C Chemokine Receptor 4 [CD184; CXCR4], clone B-T17 (Hu)

FC

Ms IgG1

Pur

100 µg

CBL701

Disialoganglioside GD2, clone 14.2Ga (Hu)

IH, IF, FC

Ms IgG2a

Pur

100 µg

MAB2052

Disialoganglioside GD3 , clone MB3.6 (Hu)

IH, IF, FC

Ms IgG3

Pur

100 µg

MAB2053

2. Rietze, R.L., et al. (2001) Nature 42:736-739.

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3

Stem Cell Markers

continued

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

Endoglin [CD105], clone MJ7/18 (Ms)

IB, IH, FC

Rt IgG2aκ

Pur

500 µg

CBL1358

Endoglin [CD105], clone 8E11 (Hu)

IH, IH(P), FC

Ms IgM

Pur

200 µg

CBL418

Endoglin [CD105; ancillary TGF-β receptor], clone P3D1 (Hu)

IB, IC, IH, IP, EIA, FC

Ms IgG

Pur

100 µg

MAB2152

Heat Stable Antigen [CD24], clone 30-F1 (Ms)

IH, IP, FC

Rt IgG2cκ

Pur

500 µg

CBL1315

Heat Stable Antigen [CD24], clone SN3 (Hu)

IH, IP, FC

Ms IgG1

Pur

100 µg

CBL561

Oct-4 [Octamer-4, Oct-3], clone 9E3 (Hu, Ms)

IB, IC, EIA

Ms IgG

Pur

100 µg

MAB4305

Nestin (Hu, Not Ms, Not Rt)

IB, IC, IH, IH(P)

Rabbit

Serum

50 µL

AB5922

Nestin, clone rat-401 (Ms, Rt, Not Hu)

IB, IC, IH, IH(P), Web* Ms IgG1

Pur

100 µg

MAB353

Stage-Specific Embryonic Antigen-1 [SSEA-1], clone MC-480 (Hu, Ms, Rt)

IH, IP, IF, FC

Ms IgM

Pur

100 µg

MAB4301

Stage-Specific Embryonic Antigen-3 [SSEA-3], clone MC-631 (Hu, Ms)

IH, IF, EIA, FC

Rt IgM

Pur

100 µg

MAB4303

Stage-Specific Embryonic Antigen-4 [SSEA-4], clone MC-813-70 (Hu, Ms)

IH, IF, EIA, FC

Ms IgG3

Pur

100 µg

MAB4304

TRA-2-49, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E (Hu, Por, Fel, Rb, Not Ms, Not Rt, Not GP, Not Mky)

IP, IF, FC

Ms IgG1

Pur

100 µg

MAB4349

TRA-2-54, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J (Hu, Por, Fel, Mky, Rb, Not Ms, Not Rt, Not GP)

IP, IF, FC

Ms IgG1

Pur

100 µg

MAB4354

TRA-1-60, clone TRA-1-60 (Hu)

IB, IH, IP, IF, FC

Ms IgM

Pur

100 µg

MAB4360

TRA-1-81, clone TRA-1-81 (Hu)

IB, IH, IP, IF, FC

Ms IgM

Pur

100 µg

MAB4381

TRA-1-85, Blood Group antigen Ok(a), clone TRA-1-85 (Hu) IB, IP, FC

Ms IgG1

Pur

100 µg

MAB4385

Vimentin (Hu, Mam)

IB, IC, IH, IH(P)

Goat

Vimentin, clone V9 (Hu, Ms, Rt, Bov, Por, Can, Chk, Fel, Mky)

IB, IC, IH, IH(P), Web* Ms IgG1

Vimentin, clone VIM3B4 ES Cell Characterization Kit (Hu, Ms, Rt)

IC

ES Cell Marker Sample Kit, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, Oct-4, clone MC-480, MC-631, MC-813-70, TRA-1-60, TRA-1-81, 9E3 (Hu, Ms)

IC

ES Cell 3D Culture Kit (Hu, Ms)

IC

Serum

500 µL

AB1620

Pur

40 µg

MAB3400

Mouse

Pur

50 µg

MAB3410

Pur

100 test

SCR001

Mouse, Rat

Pur

1 kit

SCR002

1 Kit

SCR003

Early Neuronal Markers

O

nce differentiated, neural stem cells have the potential to produce one of the three major cell types in the central nervous system: neurons, astrocytes and oligodendrocytes. Antibodies specific for neuronal or glial proteins can be utilized to identify the phenotypes of differentiated cells.

4

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

ATH 1 [MATH1] (Hu, Rodent)

IB, IH

Rabbit

APur

100 µg

AB5692

ASH 1 [MASH1] (Hu)

IB, IH

Rabbit

APur

100 µg

AB5696

HuC (Hu, Rodent)

IB, IC

Rabbit

APur

100 µg

AB5829

Internexin, α, C-terminus, localized to the terminal 164 amino acids of rat α internexin, clone 2E3 (Hu, Chk, Mam)

IB, IH(P), IP, EIA

Ms IgG1

Sup

300 µL

MAB5224

L1 Neural adhesion molecule [CD17], (Hu) clone UJ127.11

IB, IH, IP, FC

Ms IgG1

Pur

100 µg

CBL275

L1, clone 324 (Ms, Rt)

IB, IC

Rt IgG

Pur

100 µg

MAB5272

MAP1B [MAP5], clone AA6 (Ms, Rt, Bov, Chk, Fel, Hmst)

IB, IH

Ms IgG1

Asc

100 µL

MAB366

MAP1B [MAP5], clone 3G5 (Hu, Rt, Bov, Not Chk)

IB, IH, IH(P), IP, IF

Ms IgG1

Asc

100 µL

MAB376

MAP2A, 2B (Hu, Bov, Rt, Amph)

IB, IH

Ms IgG1

Asc

100 µL

MAB378

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Early Neuronal Markers

continued

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

MAP2 (Hu, Rt, Ms)

IB, IC, IH

Rabbit

Pur

100 µL

AB5622

Rt IgG1

Pur

100 µg

MAB357

Nerve Growth Factor Receptor IB, IH, IP [low affinity NGF-Receptor; p75], Extracellular, clone 192 (Rt, Not Hu, Not Ms)

Ms IgG1

Pur

50 µg

MAB365

Nerve Growth Factor Receptor [NGF-Receptor], p75 (Ms, Chk, Not Hu, Not Rt)

IB, IC, IH(P), IP

Rabbit

Serum

100 µL

AB1554

Nestin, clone rat-401 (Ms, Rt, Not Hu)

IB, IC, IH, IH(P), Web* Ms IgG1

Pur

100 µg

MAB353

100 µg

MAB5326

Rabbit anti-microtubule-associated protein 2 (MAP2) (Catalog Number AB5622). Rat hippocampal neurons in culture fixed 4 days after seeding. AB5622 was used at 1:1,000 dilution. Developed with anti-rabbit-Alexa488 secondary reagent.

Nerve Growth Factor Receptor [NGF-Receptor p75], Extracellular, clone A23816 (Ms)

IB, IC, IP

Mouse anti-Nestin (Catalog Number MAB353). Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin. The blue is Nissl stain (rough ER), the red is nestin (MAB353), and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes). Photo courtesy of Dr. Robert Wine, NIEHS.

Nestin, Human, clone 10C2 (Hu, Not Ms, Not Rt)

IB, IH, IH(P)

Ms IgG1

Pur

Nestin (Hu, Not Ms, Not Rt)

IB, IC, IH, IH(P)

Rabbit

Serum

50 µL

AB5922

NeuroD, recognizes NeuroD 1, 2 & 3, a.a. 133-148 of human NeuroD1 (Hu, Rodent)

IB, IH

Rabbit

APur

100 µg

AB5686

Neurofilament 68 kDa (Hu, Ms, Rt, Bov, Mky)

IB, IH, IH(P), EIA

Rabbit

Serum

50 µL

AB1983

Neurofilament 70 kDa, clone DP5 2.7.3 (Hu, Bov, Por, Not Ms, Not Rt)

IH, IH(P)

Ms IgG1

Pur

30 µL

MAB5294-30UL

Neurofilament 70 kDa, clone DA2 (Hu, Ms, Rt, Bov, Por)

IB, IH

Ms IgG1

Sup

300 µL

MAB1615

Neuron Specific Enolase [NSE], clone F3-1C4 (Hu, Rt, Bov) IC

Ms IgG1

Pur

100 µg

MAB314

Neuron Specific Enolase [NSE], clone 5E2 (Hu)

IB, IC, IH(P), EIA

Ms IgG2a

Pur

100 µg

MAB324

Neuron Specific Enolase [NSE; gamma enolase; Neural enolase; enolase 2], (Rt, Bov)

IB, IH, IH(P)

Rabbit

Serum

500 µL

AB951

Neuron-Specific Nuclear Protein [NeuN] (Hu, Ms, Rt)

IB, IC, IH

Ms IgG1

Pur

500 µg

MAB377

100 µg

AB5740

Mouse anti-NeuN (Catalog Number MAB377) Localization of NeuN (red) and BrdU (green) in mouse neurogenic regions (dentate gyrus and subventricular zone). Photo courtesy of J.G. Emsley and T. Hagg.

Nkx-2.2 [NK-2] (Hu, Rodent)

IB, IH

Rabbit

APur

Noggin

Coming Soon

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5

Early Neuronal Markers

continued

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

Pax-6 [Paired box protein Pax-6; Oculorhombin],

IB, IH C-terminus (Ms)

Rabbit

Serum

100 µL

AB5409

Rabbit anti-Pax6 (Catalog Number AB5409). Localization of Pax6 in a section of the embryonic mouse eye, at day 10.5 of development, showing strong Pax6 labeling of all cell nuclei in the lens, neural retina, and retinal pigmented epithelium. Photo courtesy of Dr. Grant Mastick, University of Reno, NV.

Polysialic Acid-NCAM [PSA-NCAM], clone 2-2B (Ms)

IB, IC, IH, RIA

Asc

50 µL

MAB5324

Tubulin, β III isoform, C-terminus, clone TU-20 (Hu, Ms, Rt, Bov, Por, Mky)

IB, IC, IH, IH(P), IP, EIA Ms IgG1

Ms IgM

Asc

100 µL

MAB1637

TUC-4 Protein [ULIP-1 protein; CRMP-4; DRP-3], Antibody-25 (Hu, Ms, Rt, Fel, Mky)

IB, IC, IH, IP

Rabbit

Serum

100 µL

AB5454

Tyrosine Hydroxylase [TH; Tyrosine Monooxygenase] (Ms, Rt, Fel, Fer, Moll, Not Hu)

IB, IH, IH(P), IP, EIA

Rabbit

APur

100 µL

AB152

Tyrosine Hydroxylase [TH; Tyrosine Monooxygenase], clone LNC1 (Hu, Rt, Chk, Frg, Mky, Vole)

IB, IH, IP

Ms IgG1κ

Asc

100 µL

MAB318

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

Astrocytes (Hu, Rt)

IB, IC, IH(P)

Ms IgM

Asc

100 µL

MAB5372

Early Astrocyte Markers Glial Fibrillary Acidic Protein [GFAP] (Rt)

IC

Chicken

APur

50 µL

AB5541

Glial Fibrillary Acidic Protein [GFAP] (Hu, Rt, Bov, Can)

IC, IH

Rabbit

Serum

50 µL

AB5804

Rabbit anti-GFAP (Catalog Number AB5804). Staining of cultured neonatal rat forebrain with the anti-GFAP is shown in green, while neuronal processes were identified using a monoclonal antibody to the neurofilament subunit α-internexin (Catalog Number MAB5224) are shown in red.

6

Glial Fibrillary Acidic Protein [GFAP], clone GA5 (Hu, Ms, Rt, Bov, Por, Chk, Rb)

IB, IC, IH, IH(P), Web* Ms IgG1

Pur

40 µg

MAB3402

Glial Fibrillary Acidic Protein [GFAP], Prediluted (Hu)

IH(P)

Rabbit

Pur

6 mL

IHC2078-6

Glutamine Synthetase, clone GS-6 (Rt, Sh)

IB, IH, EIA

Ms IgG2a

Pur

100 µg

MAB302

HES-1 [Hairy 1], Available Summer 2004 (Hu, Rodent)

IB, IH

Rabbit

APur

100 µg

AB5702

Notch1, clone mN1A (Ms, Rt, Fel)

IB, IC, IH, IP

Ms IgG1

Pur

100 µg

MAB5352

Notch1 (Ms, Rt)

IB, IC, FC

Hmst

Sup

100 µL

MAB5414

S-100 Protein (Hu)

IH(P)

Rabbit

Serum

100 µL

AB941

S-100 Protein, clone 15E2-E2-A1 (Hu, Ms, Rt, Bov, Can, Fel, Rb)

IH, IH(P), IP

Ms IgG2aκ

Asc

500 µL

MAB079

S-100 Protein, clone 15E2-E2-A1 (Hu, Ms, Rt, Bov, Can, Fel, Rb)

IH, IH(P), IP

Ms IgG2aκ

Pur

100 µg

MAB079-1

S100, Prediluted (Hu)

IH(P)

Rb Polyclonal

Pur

6 mL

IHC2137-6

S100L Protein [S100A2], clone S100-14 (Hu, Bov)

IB, EIA

Ms IgG

Pur

150 µg

MAB385

Vimentin, clone J144 (Hu)

IH

Ms IgG

Asc

100 µL

MAB1633

Vimentin, clone LN-6 (Hu, Ms)

IH(P)

Ms IgM

Sup

1 mL

MAB1681

Vimentin, clone 43BE8 (Hu, Mky)

IC, IH(P)

Ms IgM

Asc

100 µL

MAB1687

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Oligodendrocyte Markers Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

CNPase [2'3'-Cyclic Nucleotide 3'-Phosphohydrolase], clone 11-5B (Hu, Ms, Rt, Bov, Por, Can, Rb, Sh, Not Chk, Not GP)

IB, IC, IH, IH(P)

Ms IgG1

Pur

100 µg

MAB326

Disialoganglioside GD3 [GD3], clone MB3.6 (Hu)

IH, IF, FC

Ms IgG3

Pur

100 µg

MAB2053

Galactocerebroside [GalC; galactoceramide] (Hu, Ms, Rb, Rt)

EIA, IC, IH, Web*

Ms IgG3

Pur

50 µg

MAB342

Myelin Basic Protein [MBP], a.a. 119-131, clone 2 (Hu, Rt, Bov, GP, Rb, Sh)

IC, EIA

Ms IgG1

Sup

2 mL

MAB381

NG2 Chondroitin Sulfate Proteoglycan (Rt)

IB, IH, EIA

Ms IgG1

Pur

100 µg

MAB5384

NG2 Chondroitin Sulfate Proteoglycan (Hu, Ms, Rt, Mky)

IB, IC, IH, IP, EIA

Rabbit

Pur

100 µg

AB5320

Rabbit anti-NG2 (Catalog Number AB5320) Staining of adult rat hippocampus. Photo shows single oligodendrocyte precursor cell.

Nkx-2.2 [NK-2] (Hu, Rodent)

IB, IH

Rabbit

APur

100 µg

AB5740

O1, clone 59 (Hu, Ms, Rt, Chk)

IH, Web*

Ms IgM

Pur

50 µg

MAB344

O4 [sulfatide], clone 81 (Hu, Ms, Rt, Chk)

IC, IH, Web*

Ms IgM

Pur

50 µg

MAB345

Olig1 (Hu, Rodent)

IB, IC

Rabbit

APur

100 µg

AB5991

Olig3 (Hu, Rodent)

IB, IC, IH

Rabbit

APur

100 µg

AB5995

Oligodendrocytes [MOSP], clone CE-1 (Hu, Ms, Rt, Chk, Fel, Mky)

IC, IH, IH(P), IP, not IB Ms IgM

Asc

100 µL

MAB328

Asc

100 µL

MAB1580

Mouse anti-oligodendrocytes (Catalog Number MAB328). Oligodendrocytes from neonatal rat.

Oligodendrocytes [RIP], clone NS-1 (Ms, Rt, Hmst, Not Hu) IC, IH

Ms IgG

Mouse anti-Oligodendrocytes (Catalog Number MAB1580) Staining of adult rat cortex.

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7

Engraftment Analysis

T

he potential of neural stem cells to be utilized in the treatment of various neuropathies is demonstrated by their successful implantation into neonatal immunodeficient rodents. Engrafted neurons derived from transplanted human neural stem cells can be discriminated from host tissue using antibodies specific for human N-CAM or human nuclei. Proliferating cells of human origin can be detected using an antibody specific for the human nuclear protein Ki-67. This protein is expressed by human cells in all phases of the active cycle and is absent in the resting phase.

Once engrafted, the differentiation of neural stem cells is dependent upon local cues in the host brain. Apart from a few exceptions, neurogenesis is completed soon after fetal development. Consequently, the brain tissue of adult graft recipients lacks the necessary cues needed to drive the differentiation of implanted stem cell progeny. One method to overcome this is the pre-differentiation of neural stem cells prior to implantation. The detection of implanted, pre-differentiated cells can be accomplished by labeling of proliferating and differentiating cells with BrdU prior to implantation. Resulting neurons and glia can be detected using an anti-BrdU antibody.

Mouse anti-Golgi Zone (Catalog Number MAB1271) Staining of Golgi Zone (green) in human neural stem cells in vitro. Nuclear counterstaining with Hoechst (blue).

Figure 1. Mouse anti-neurofilament 70 kDa (Catalog Number

Figure 2. Mouse anti-neurofilament 70 kDa (Catalog

Mouse anti-human nuclei (Catalog Number

MAB5294) localization in a graft of transplanted fetal porcine

Number MAB5294) taken at 10x using dark field

MAB1281) staining of human neural stem cells

ventral mesencephalon cells in the rat corpus striatum, 15

optics shows the same graft in greater detail to

transplanted into rat brain. Photo courtesy of

weeks after transplant (4x). The asterisk shows the area

highlight the NF70-immunoreactive porcine neuronal

ReNeuron.

magnified in Figure 2.

fibers growing out from the graft core into the rat striatum.

Antibody/Known Species Reactivity

Known Applications

Host/Subclass

Format

Quantity

Catalog Number

Bromodeoxyuridine [BrdU] (All Species)

IH, FC

Ms IgG1

Pur

50 µg

MAB3424

Bromodeoxyuridine [BrdU] (All Species)

IC, FC

Ms IgG

Sup

100 µL

MAB3510

Bromodeoxyuridine [BrdU] (All Species)

IB, IC, IH(P), FC

Ms IgG1

PSup

100 µg

MAB3222

Golgi Zone (Hu)

IC, IH

Ms IgG1

PSup

100 µL

MAB1271

Ki-67 (Hu, Rt, Rb, not Ms)

IH, FC

Ms IgG1

Sup

1 mL

MAB4062

Ki-67 (Hu)

IH, FC

Ms IgG1

Pur

100 µg

MAB4190

Neurofilament 70 kD (Hu, Bov, Por, Not Rt, Not Ms)

IH

Ms IgG1

Asc

30 µL

Neural Cell Adhesion Molecule [NCAM] (Hu)

IB, IH, IP

Ms IgG1

Asc

100 µL

MAB2122

Neural Cell Adhesion Molecule [NCAM] (Hu)

IB, IH, IP

Rt IgG2a

Pur

100 µg

MAB310

Neural Cell Adhesion Molecule [NCAM] (Hu, Ms, Chk)

IB, IH, IH(P), BLK

Rabbit

APur

50 µg

AB5032

Nuclei (Hu, not Ms, not Rt)

IH, IP

Ms IgG1

PSup

100 µL

MAB1281

MAB5294-30UL

1030-1003

[email protected] / www.ctr.com.mx / Conm. 01 81 81580600 (20 Líneas)