Rinku Dutta, Ph.D., M.Tech. Home Address: 120 S 38th Avenue, Apt # 22, Office Address: University of Nebraska Medical Center Omaha, Nebraska 68131, USA DRC II Room 6022, Omaha, NE 68198-5965 Mobile: (1) 901-517-9429 Work phone: (1) 402-559-6987 Email: [email protected], [email protected] Date of birth: August 29th, 1980. EDUCATION •

North Dakota State University, Fargo, ND, USA

(GPA = 4.0/4.0)

Doctor of Philosophy (Ph.D.), Pharmaceutical Sciences. •

2008–2012

University College of Science and Technology, University of Calcutta, INDIA (86 %) Master of Technology (M.Tech.), Pharmaceutical and Fine Chemical Technology. 2006–2008

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University College of Science and Technology, University of Calcutta, INDIA (82 %) Bachelor of Technology (B.Tech.), Pharmaceutical and Fine Chemical Technology. 2002–2005

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University of Calcutta, INDIA

(58 %)

Bachelor of Science (B.Sc.), Chemistry (Honors).

2000–2002

PROFESSIONAL AND RESEARCH EXPERIENCES •

Chemist (Quality Assurance), Albert David Limited, Kolkata, INDIA

2005-2006

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M. Tech. project, Indian Institute of Technology, Kharagpur, INDIA

2007-2008

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Research Assistant, North Dakota State University, Fargo, ND, USA

2008-2012

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Research Associate, Institute of Chemical Technology, Matunga, Mumbai, INDIA 2012-2013

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Research Scientist, Pharmaceutical Analytical R & D, Piramal Enterprises Ltd., Mumbai, INDIA

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2013-2014

Postdoctoral Research Scholar, University of Nebraska Medical Center, Omaha, Nebraska, USA 2014-Current Date

SUMMARY OF ACQUIRED TECHNICAL SKILLS •

Nanoparticle (lipid-based and polymer-based) synthesis and characterizations.

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Organic syntheses and chemical characterizations of small molecules, copolymers, peptides and liposomes. Thin layer chromatography, compound purifications using flash chromatography and NMR techniques.

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Analytical Instrument handling: Nuclear Magnetic Resonance (1H, 13C, DEPT, 2D (COSY,NOESY)), FT-IR, Mass Spectrometry (MS), High Performance Liquid Chromatography (HPLC), UV-Vis Spectroscopy, Microwave-assisted Peptide Synthesis, Fluorescence Spectroscopy (including lifetime studies), Circular Dichroism (CD), Isothermal Microcalorimetry

(ITC), Differential Scanning Calorimetry (DSC), Dynamic Light Scattering (DLS), Zeta potential, Gel filtration and Gel Permeation Chromatography, Particle size analyzer, Dissolution and Disintegration apparatus. •

Chemical assays, Immunoassays (ELISA), Inhibition assays.

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Cell culture techniques, aseptic handling of cells, cell viability assays (MTT).

RESEARCH EXPERIENCES (Post-doctoral Research Associate)

2012-2013

Research Topic (sponsored project of Indian Council of Medical Research (ICMR), in collaboration with Bombay Veterinary College and Institute of Chemical Technology (ICT), Matunga, Mumbai): “Evaluation of targeted nanoparticulate dug-delivery system in clinical cases of tuberculosis in bovines and non-human primates” under the guidance of Prof. P.V. Devarajan, Head, Dept. of Pharmaceutical Sciences and Technology, ICT, Matunga, Mumbai. • Preparation of polymer-based nanoparticle formulation (using nanoprecipitation and EmulsionSolvent Diffusion method) and characterization by UV-Visible Spectrophotometry, Photon Correlation Spectroscopy(PCS). Method development using RP-HPLC. • Scale-up of the optimized formulation for in-vivo studies. RESEARCH EXPERIENCES (Ph.D.)

2008-2012

Doctoral Research Title (project of National Institute of Health (NIH), USA in collaboration with National Cancer Institute, USA): “Detection of Metalloenzymes Employing Fluorescent-Polymers and Liposomes)” under the guidance of Prof. Sanku Mallik, Dept. of Pharmaceutical Sciences, North Dakota State University, Fargo, USA. • •

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Syntheses of encapsulated Liposomes (thin film hydration techniques), their extrusion, size determination using Dynamic Light Scattering (DLS) and Transmission electron microscopy. Syntheses of conjugated peptides on solid support (solid phase peptide syntheses (SPPS), benchtop deprotections, optimization of synthetic protocols of fluorophore-conjugated peptides and small molecules. Peptide purifications using Semi-prep HPLC (RP), qualitative determination using MALDI-ToF, UV-Vis spectroscopy, Differential Scanning Calorimetry (DSC). Syntheses of Boc-protected molecules; amide coupling (EDC-NHS; HOBT-HBTU; BOP-NMM); deprotections; “click” reactions (azide-alkyne, 1,3 dipolar cycloadditions). Design of small inhibitor molecules (hydroxamates, naphthalenesulphonamides, azides, methacrylamide-based amino acids, amines, dansylamide derivatives). Free radical copolymerizations of synthesized monomers using azobisisobutyronitrile as initiator, Mw/Mn determination using Gel permeation Chromatography. Step-wise compound purifications using solvent extraction, distillation, preparative chromatography (thin layer, column chromatography, flash chromatography). Chemical Characterizations of organic molecules via chromatographic techniques, NMR (1H, 13C and DEPT), FT-IR, and Mass Spectrometry. Syntheses of encapsulated Liposomes (thin film hydration techniques), their extrusion, size determination using Dynamic Light Scattering (DLS) and Transmission electron microscopy. Enzymatic studies included fluorescence spectroscopy, lifetime studies, inhibition and acid phosphatase assays. Protein assays (Bradford, Lowry). Aseptic cell culture handling and growth of anchorage-dependent various cell lines (MCF-7, MDA-MB-231, HeLa, PANC-1, HEK 293, PC-3, and 22Rv1), MTT assays, neutral red colorimetric assays, cryopreservations, primary monolayer cultures.

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Knowledge of statistical tools viz. Linear Discriminant Analyses (LDA), Logit Binary Regression Analyses, ANOVA, t-tests, chi-square tests. Thorough knowledge of various Pharmacokinetics and Pharmacodynamics, receptor-ligand binding, Cyp-450 and metabolites, MMP and CA enzymes.

RESEARCH EXPERIENCES (M.TECH.)

2007-2008

M.Tech. (University of Calcutta) project as a partial fulfillment of the degree gained one year research experience (2007-2008) at the Indian Institute of Technology, Kharagpur (Department of Biotechnology) on development and applications of “smart” polymer Poly-(N-isopropylacrylamide) (NIPAM). • Syntheses and characterizations of various modified hydrogels via cross-linking with NIPAM and methylene-bis-acrylamides. • Studied controlled release of drug (Hydrocortisone) and protein (Bovine Serum Albumin; BSA) from the hydrogels for further applications as scaffolds in tissue engineering. PUBLICATIONS •

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Rinku Dutta, Michael Scott, Manas Haldar, Bratati Ganguly, D. K. Srivastava, Daniel Friesner, Sanku Mallik. “Fluorescent Water Soluble Polymers for Isozyme-Selective Interactions with Matrix Metalloproteinase-9”. Bioorganic and Medicinal Chemistry Letters, 2011, 21, 20072010. Michael D. Scott, Rinku Dutta, Manas K. Haldar, Bin Guo, Daniel L. Friesner, Sanku Mallik. “Differentiation of Prostate Cancer cells by Using Flexible Fluorescent Polymers”. Analytical Chemistry, 2012, 84, 17-20. Michael D. Scott, Rinku Dutta, Manas K. Haldar, Daniel L. Friesner, Sanku Mallik. “Fluorescent Polymer-based Rapid Sub-typing of Breast Cancer Cells”. Analyst, 2012, 137, 5487-5490. Michael D. Scott, Manas K. Haldar, Rinku Dutta, Sanku Mallik. “Natural Product Activators of Sirtuins against Aging” (Book Chapter, 2013) Natural Bioactive Molecules: Impacts and Prospects. Chapter III. Editor: Dr. Goutam Brahmachari, Prof. of Chemistry, Dept. of Chemistry, Visva-Bharati University. Padma V. Devarajan, Shilpa Dawre, Rinku Dutta. ” Targeted Drug Delivery-Concepts and Design” (Book Chapter) Targeted Delivery Approaches for infectious Diseases. Part II. Chapter III. Editor: Prof. Padma V. Devarajan. Springer Publishing (2014).

PODIA PRESENTATION •

Rinku Dutta, Michael D. Scott, Manas K. Haldar, Bratati Ganguly, Daniel Friesner, D. K. Srivastava, and Sanku Mallik; “Isozyme Selective Interactions of Metalloenzymes with Fluorescent Polymers.” 43rd Annual Pharmaceutics Graduate Student Research Meeting (PGSRM) 2011, June 24, 2011, Rennebohm Hall, Madison, University of Wisconsin-Madison, WI, USA.

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Rinku Dutta, Sanku Mallik. “Detection of Metalloenzymes employing water soluble fluorescent polymers and liposomes.” 6th International Conference On Innovation in 21st Century: Clinical Pharmacology In Current And Future Environment” organized By South Asian ChapterAmerican College of Clinical Pharmacology April (21-22) 2013, Worli, Mumbai, India.

POSTER PRESENTATIONS

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Rinku Dutta, Michael D. Scott, Dr. Manas Haldar, Bratati Ganguly, Dr. D. K. Srivastava, and Dr. Sanku Mallik,“Synthesis of Fluorescent Polymers for Isozyme Selective Binding to Matrix Metalloproteinases.” 42nd Annual Pharmaceutics Graduate Student Research Meeting (PGSRM) 2010, June 18, 2010, The Ohio Union at The Ohio State University, Columbus, OH, USA.

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Rinku Dutta, Michael D. Scott, Dr. Manas Haldar, Bratati Ganguly, Dr. D. K. Srivastava, Daniel Friesner and Dr. Sanku Mallik, “Synthesis of Fluorescent Polymers for Selective Detection of Matrix Metalloproteinases Isozymes.” NDEPSCOR State Conference, September 29, 2010, UND, Alerus Centre, Grand Forks, USA.

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Rinku Dutta, Dr. Manas Haldar, and Dr. Sanku Mallik, “Synthesis of Luminescent Polymers for Protein Surface Recognition.” 41st Annual Pharmaceutics Graduate Student Research Meeting (PGSRM), June, 2009, Purdue University, West Lafayette, IN, USA.

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Rinku Dutta, Dr. Manas Haldar, Bratati Ganguly, Dr. D. K. Srivastava, and Dr. Sanku Mallik, “Synthesis of Fluorescent Polymers for Protein Surface Recognition”. Experimental Program to Stimulate Competitive Research (EPSCor) 2009 State Conference, September 24th 2009, NDSU Memorial Union, Fargo, ND, USA.

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Rinku Dutta, Dr. Manas Haldar, Michael D. Scott, Bratati Ganguly, Dr. D. K. Srivastava, and Dr. Sanku Mallik, “Synthesis of Fluorescent Polymers for Protein Surface Recognition for MMPs detection.” 2nd Annual NDSU Graduate Research and Arts Forum, April 21, 2010, NDSU Memorial Union, Fargo, ND, USA.

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Michael D. Scott, Manas K. Haldar, Rinku Dutta, Bratati Ganguly, D. K. Srivastava, and Sanku Mallik, “Using of Fluorescent Polymers to detect Matrix Metalloprotease (MMP-9).” 2nd Annual NDSU Graduate Research and Arts Forum, April 21, 2010, NDSU Memorial Union, Fargo, ND, USA.

1. PROFESSIONAL EXPERIENCES

2005-2006

Quality Assurance Chemist (June 2005 – September 2006) at Albert David Limited, Kolkata, INDIA. Preparation and implementation of QA related systems, ensure adherence and regulatory compliance. • • • • • • • • •

Prepare of Site Master File, Validation Master Plan. Stability study of finished products according to internal protocol as well as ICH guidelines. Review and management of QMS activities (Training records, Deviation, Investigation, Change control, Internal audits). Participate in Technology transfer during shop floor manufacturing of drug products; protocol preparation, execution and reporting of process validations, trend analysis. Prepare and review Batch Manufacturing Record; release of finished products. Data compilation for Annual Product Quality Review. Pre-audit preparedness review for regulatory inspections. Prepare regulatory audit compliance response; review CAPA execution. Ensure cGMP, GLP practice during routine manufacturing operations.

2. PROFESSIONAL EXPERIENCES

2013-2014

Research Scientist (September 2013-July 2014), Analytical Pharmaceutical R & D (injectables) at

Piramal Enterprises Limited, Mumbai, INDIA. • Lyophilised peptide and liposome formulation development and preparation. • Analytical method development, method validation and tech transfer. • Execution and following of Good Laboratory Practices (GLP). • Regulatory compliances and submissions. 3. PROFESSIONAL EXPERIENCES

2014-Current date

Postdoctoral Research Scholar (August 2014-Current Date), under Prof. Ram Mahato. Design and synthesis of monomeric entities and polymers, micelle and lipopolymers for targeted drug and gene delivery (comprising of miRNA, siRNA, oligonucleotides) for the treatment of liver fibrosis and cancer. AWARDS AND ACHIEVEMENTS • • •

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Topper of the year 2005, B.Tech. (Chemical Technology Department in the Pharmaceutical and Fine Chemical Division, University of Calcutta, INDIA) Ranked 2nd in the class 2008, M.Tech (Pharmaceutical and Fine Chemical Technology, University of Calcutta, INDIA). Received presitigous “Sibley Scholarship” in 2008 from The University of Calcutta, INDIA. Outstanding achievement in research and academics Award (2011) by PRACS, Cetero and College of Pharmacy, Nursing and Allied Sciences, North Dakota State University, ND, USA.

References : (1) Dr. Sanku Mallik Professor, Department of Pharmaceutical Sciences, College of Pharmacy, Nursing and Allied Sciences, North Dakota State University, Sudro Hall, Room 35A Dept. 2665, PO Box 6050, Fargo, ND 58108-6050, USA. Phone: 1-701-231-7888 E-mail: [email protected] (2) Dr. Jagdish Singh Professor and Head, Department of Pharmaceutical Sciences, College of Pharmacy, Nursing and Allied Sciences, North Dakota State University, Fargo, ND, USA. Phone: 1-701-231-7943 E-mail: [email protected]

(3) Dr. Padma V. Devarajan Professor and Head, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, N.P. Marg, Matunga, Mumbai 400019, MH, India. Phone: 91-2233612201 E-mail: [email protected] (4) Dr. Vandana J. Sonavaria Vice President, Pharmaceutical R & D, Piramal Enterprises Limited, 1 Nirlon Complex, Off Western Express Highway, Goregaon East, Mumbai 400063, MH, India. Mobile: 91-9321490402, Work phone: 91-22-3081-8201 E-mail: [email protected]