PCR & Molecular Biology Certified High-Quality Products for PCR, Molecular Biology and Research

sterile · non-pyrogenic · DNA-free RNase-free · ATP-free

Liquid Handling

SARSTEDT International Your Partner in medicine and science worldwide

Table of Contents

Sarstedt quality symbols

A warranty of reliability and reproducibility

p. 4

Biosphere Purity Certificate

p. 4

PCR Performance Tested Purity Certificate

p. 4

®

Pipette tips

Crucial for precise dosing

p. 5 - 19

Aerosol filters – Protection against carry-over contamination

p. 5

Quality guarantee by conformity testing at regular intervals

p. 6

Superior shielding with the special ShaftProtect 10µl tip

p. 7

Filter tips – Pipetting small volumes from 0.1 – 20µl Filter tips – Pipetting sample volumes from 1 – 1,250µl

p. 10 - 11

TipStack Pack reload system

p. 12 - 13

Tip SystemBox - Central pipetting station and reload trays

p. 12

Tip StackPack / Starter Kit – the economic reload system

p .13

Compatibility Table

p. 14 - 17

Ordering information on quality pipette tips with and without filter

p. 18 - 19

Robotic tips

Tips for Biomek pipetting platforms

p. 20

Multiply® reaction tubes

PCR tubes, strips of 4 and 8 tubes

p. 21 - 23

Product description of Multiply PCR tubes, strips and lids

p. 21

Ordering Information for Multiply PCR tubes, strips and lids

p. 22

Accessories: The interchangeable RackSystem for sample preparation

p. 23

®

®

Multiply® PCR plates

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

11

p. 8 - 9

White and transparent options in 96/384 well format

New

12

White PCR plates optimised for qPCR Ordering information for white PCR plates and optical lids

H

New

Adhesive sealing tapes

New

p. 26 p. 27 - 28

Ordering information for transparent PCR plates and lids

p. 29

Free from DNA, DNase/RNase and endotoxins For qPCR/PCR, automation and sample storage

New New

p. 24 - 26

Transparent PCR plates, w/o skirt, with half and full skirt

Compatibility Table

96 Deep well MegaBlock®

p. 24 - 31

p. 30 - 31

p. 32 p. 33 - 35

Highly transparent, adhesive qPCR tape

p. 33

Transparent tape for end point PCR/storage, strongly adhesive

p. 34

Aluminium tape for PCR and sample storage

p. 34

Pierceable tape for DNA sequencers, HPLC, autosamplers

p. 35

SafeSeal micro tubes

0.5/1.5 and 2.0 ml with secure cap locking feature

p. 36 - 37

UV transparent single-use cuvettes

For photometric analyses ≥ 220 nm

p. 38 - 39

Sarstedt quality symbols - a warranty of reliability and reproducibility

To accommodate the highly sophisticated needs of customers working in the field of medical research, environmental technology, the pharmaceutical and food industries as well as in cellular and molecular biology, Sarstedt has developed the Biosphere® quality products. The superior biological purity of sterile laboratory products is guaranteed by a controlled production process that complies with the strictest purity requirements so that our customers can be sure to achieve utmost reproducibility in their analyses and research experiments.

All disposables, such as pipette tips and reaction tubes bearing the Biosphere® symbol are sterile, DNA-, RNase- and ATP-free and non-pyrogenic. To reduce storage time of the sterile Biosphere® products in open bags and inherent contamination risks, Biosphere® tubes and plates are packaged in small quantities. Biosphere® laboratory products are manufactured in accordance with defined production requirements and meet the following threshold values: DNA < 2 x 10-14 g ATP < 3.5 x 10-15 g

RNase < 1.1 x 10-11 Kunitz units Pyrogens < 2 x 10-3 EU

Sterility is validated according to ISO 11135.

PCR and other molecular-biological methods have gained access to laboratories all over the world. Sarstedt has enormously refined the production requirements for non-sterile PCR products. Skilled personnel, clean room conditions along with automated production processes enable contamination-free manufacturing.



Each production batch is subjected to a highly sensitive PCR method to guarantee compliance with the individual quality criteria. Consequently, award of the PCR Performance Tested quality symbol to a production batch is subject to successfully passing PCR tests. Thanks to this quality grade, customers can use Sarstedt disposables in direct contact with reagents and sample material for PCR in their experimental work. Products awarded the PCR Performance Tested standard meet the following requirements:



4



3 DNase-/RNase-free

3 Human DNA-free

3 PCR inhibitor-free

High purity Biosphere® Filter tips for pipetting – free from carry-over contamination Protection against carry-over and contamination Even the most meticulous pipetting may generate aerosols during aspiration of the sample, contaminating the pipette and any sample material to follow. This cross-contamination cannot be tolerated when working with expensive, hazardous, or irreplaceable samples or when using highly sensitive methods. In order to prevent aerosol contamination along with the resulting consequences (falsified results, additional expenditure in time and cost) Sarstedt incorporates special porous elements in its range of filter tips.

Reliable safety with selected filter materials The filter element integrated in the pipette tips consists of a porous plastic material featuring defined hydrophobic properties and no self-sealing additives. • The sponge-like material is densely composed of randomly arranged channels (see picture on left), enabling air to pass for precision when using air displacement pipettes. • Simultaneously, the complex structure of the porous filter safely prevents aerosols from entering the pipette shaft.

Cross section of a porous filter element (40-fold magnification)

Measuring the efficiency of filter inserts in Biosphere® filter tips* Both the PCR-based method and indirect, radioactive measurement of the plasmid DNA with P-32 marked ATP are reliable methods for detecting carry-over contamination of DNA aerosols likely to occur in practical pipetting applications.

piston pipette shaft sealing surface of pipette tip

aerosol-free

filter aerosol

• With both methods, the hydrophobic filter inserts with the selected porosity used in Sarstedt Biosphere® filter tips were found to provide protection against aerosol contamination. • The superior efficiency of the filter in Biosphere® filter tips is based on a complex mechanism of absorption, surface quality, thickness, strength and shape.





* Refer to our “Application Report – Shielding Effect of Filter Inserts in Pipette Tips” for additional details

sample

Added protection with Biosphere® purity quality 3 Maximum biological purity: free from DNA, RNase, ATP and pyrogens 3 Batch-specific certification is available upon request

Convenient and safe packaging of individual tip boxes 3 Each tip box is additionally and aseptically protected in a special



sterile bag packaging

3 Easy opening of bag packaging without aid via notch at short bag



side (see picture)

5

The pipette tip – Crucial for precise dosing A calibrated system: Pipette and pipette tips The precision of the pipetting process is primarily determined by the optimal compatibility of the pipette and pipette tip. • One of the leading manufacturers of pipette tips, Sarstedt’s main objective for years has been to provide customers with tips in optimal, reliable quality to fit most marketable pipette brands. • The quality of a tip is defined by the properties of the material used, the design and optimum fit on the individual pipettes.

Quality guarantee: Precision and accuracy for small volumes In particular, the ongoing trend towards ever decreasing small sample volumes inevitably involves increasing demands on the dosing system. At regular intervals, Sarstedt tips are checked for maximum precision and reliability in combination with the pipettes of leading manufacturers (e.g. Eppendorf, Gilson). In addition, the tips are subjected to a defined procedure of comprehensive in-process conformity testing in accordance with the international standard EN ISO 8655 for piston pipettes.

Note on availability of a

!

Declaration of Conformity for pipette/pipette tip combinations

•

The Declaration of Conformity for piston pipettes indicates the combination of pipette and pipette tip to which it applies attesting precision and reliability and can be made available on request.

• The logo on the label indicates that a standard Declaration of Conformity is available for a particular pipette tip.

6

Precise pipetting of micro volumes - superior precision and contamination protection with the ShaftProtect 10 µl tip for Gilson P2/P10 With most extra long tips the dead volume is extended, which creates adverse effects on performance but ShaftProtect 70.1131 has been especially developed for Gilson P2/P10 pipettors.

ShaftProtect 10 µl tip Filter tip 70.1131.210

0.1 - 10 µl

ro tP af Sh ct te

• Extra-long tip with ShaftProtect for Gilson P2/P10 to protect the inner tube wall against the generally non-sterile pipette shaft • Identical precision and accuracy thanks to a very small dead volume comparable to the short tip 70.1130

10µl 2µl

• Enhanced dosing features Especially fine, extra-long 'ShaftProtect' 10 µl tip for easy dosing of even the smallest volumes • Easy visual control Calibration rings facilitate visual control for micro volumes • Conformity-tested to EN ISO 8655

!

• Colour code

Safe, precise and accurate pipetting down to the base of 1.5/2.0 ml micro tubes with the ShaftProtect 10 µl tip for Gilson P2/P10 • Flexibility & convenient handling With the dual position tip-ejector* (see illustration), the extended ShaftProtect tip covers as much of the pipette shaft as the short 70.1130.

* All new (since 2003) P2/P10 pipettors from Gilson are equipped with dual position tip-ejector, allowing the use of short tip 70.1130.xxx and „Gilson long“ 70.1131.xxx. Older P2/P10 models need the Gilson ”Service Kit 2”.

Dual position tip-ejector twisted by 180° (Pos. 1/Pos. 2) in axial direction allowing choose between the long 70.1131 and short 70.1130 tips

Standard 10 µl tip

sterile non-sterile

180°

Pipette cone sealing position

70.1131

70.1130

Dual position tip-ejector Pos. 2

Dual position tip-ejector Pos. 1



ShaftProtect 10µl tip

7

The pipette tip – Crucial for precise dosing

Pipetting small sample volumes 0.1 – 10 µl Working routinely with micro volumes can adversely affect the pipette / pipette tip dosing system. Therefore, tips to 10 µl feature the following product benefits of enhanced visual control and dosing: • Enhanced pipetting safety due to calibrated system - conformity-tested to EN ISO 8655 • Easy visual control for micro volumes - calibration rings facilitate visual control of the pipetted volume • Enhanced dosing features - fine, thin tip facilitates dosing and visual volume control

70.1130.210/.212

Filter tip 70.1130.210/.212

0.1 - 10 µl / 0.1 - 2.5 µl

Mini Gilson tip for Gilson P2/P10 or Eppendorf Research 2.5 µl with finely drawn tip and calibration rings in 2 and 10 µl tips, as well as for versions of identical design from Finnpipette, Biohit and Socorex. 10 µl 2 µl

•

Conformity tested to EN ISO 8655

•

Colour code

!

!

70.1116.210

10 µl 2 µl

8

0.5 - 20* µl

Universally compatible with Gilson, Eppendorf, or identical designs, e.g . Biohit, Finnpipette, Abimed and Brand for volumes between 0.1 and 10 µl. The fine, extra-long tip and calibration rings facilitate dosing and visual volume control.

20 µl

!

Filter tip 70.1116.210

* Compatible with Eppendorf Reference 2 - 20 µl and identical pipette models from other manufacturers. •

Conformity tested to EN ISO 8655

•

Colour code

!

Pipetting micro volumes from 0.5 to 20 µl – specially designed tip for perfect fit on Eppendorf pipettes Tip with ergonomical and flexible cone, enhanced sealing properties and outstanding precision on Eppendorf pipettes. The cone geometry enables optimal fit and effortless dosing with new generation single- or multi-channel pipettes (e.g. Research plus pipettes). The tip is also compatible with identical pipette models from other manufacturers.

New

Filter tip 70.1114.210

0.5 to 20 µl

Compatible with 0.5 - 10 µl Eppendorf Research, Research plus, Research pro, Xplorer, Reference 2 - 20 µl and Finnpipette Digital 0.2 - 2 µl, as well as models of identical design.

• 46 mm tip length enables safe pipetting down to

20µl 10µl 2µl



the bottom of deep vials

•

Cone geometry optimised for 8- and 12-channel pipettes

•

Graduation rings without interfering ridges for easy dosing into vials

•

Extra thin tip for enhanced dosing of micro volumes

•

Visual control of micro volumes Graduation rings at 2, 10 and 20 µl

•

Conformity tested to EN ISO 8655

•

Colour code

Now available with perforated temper-evident closure

NEW

70.1115.210

!

Filter tip 70.1115.210

0.5 - 10 µl

Type “crystal tip” for Eppendorf 0.5 - 10 µl, Reference/Research or identical designs, e.g. Finnpipette. •

Conformity tested to EN ISO 8655

•

Colour code

!

!

9

The pipette tip – Crucial to precise dosing

Pipetting sample volumes 2 - 300 µl 70.760.212/.213

Filter tip 70.760.212/.213

2 - 100 µl

Yellow standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Brand, Socorex as well as identical designs, and additional filter tip option of 20 µl volume (70.760.213). •

Conformity tested to EN ISO 8655

•

Colour code

!

!

70.760.211

Filter tip 70.760.211

2 - 200 µl

Standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Finnpipette, Socorex and identical designs. •

Conformity tested to EN ISO 8655

•

Colour code

!

!

70.765.210 300 µl 200 µl 100 µl 50 µl

!

10

Filter tip 70.765.210

10 - 300 µl

Specially suited for multi-channel pipettes, e.g. from Eppendorf, Brand, Finnpipette, Biohit. Also compatible with older multi-channel pipettes up to 200 µl. 55.6 mm tip with 4 graduations for visual control of pre-set pipetting volume at 50, 100, 200 and 300 µl. •

Conformity tested to EN ISO 8655

•

Colour code

!

Pipetting large sample volumes up to 1,250 µl •

Two pipette tips with graduation rings for volumes between 1 and 1,250 µl

•

Saves space as a large volume range is covered with 2 tips only

•

Colour-coded trays enable easy volume identification

•

Highly transparent tip material for enhanced pipetting control

70.1189.215

Filter tip 70.1189.215*

1 - 200 µl, extra long

Extra long and slim 200 µl tip for 20, 50, 100 and 200 µl pipettes. Designed for universal use on a pipette volume range between 2 and 200 µl: Eppendorf Reference, Research, Biohit mLine, -Proline, -Proline Electronic, HTL, Labnet, Finnpipette, Nichiryo Benchmate, Gilson, Rainin, CappAero, CappTronic Electronic and identical designs.

200 µl 100 µl 50 µl 10 µl

NEW

70.762.211

- - - -

Soft, flexible cone for easy assembly and ejection 77 mm length for reaching into tubes One pipette accommodates 4 different volumes Graduation rings without interfering ridges at 10, 50, 100 and 200 µl for visual control of the defined pipetting volume

•

Colour code

Filter tip 70.762.211 50 - 1,000 µl Blue standard tip for Eppendorf, Gilson, Finnpipette, Biohit, Brand, Socorex and identical designs. - Empty box without pipette tips 95.1762, 10 pcs./case •

Conformity tested to EN ISO 8655

•

Colour code

!

!

70.1186.210

1,000 µl 500 µl 200 µl

Filter tip 70.1186.210

50 - 1,250 µl, extra long

Extra long (103 mm) and slim pipette tip, universally suited for use with single-channel pipettes, e.g. from Gilson, Biohit, Nichyrio and Finnpipette. Particularly recommended for applications in Deep Well blocks, Cluster tubes or 5 ml CryoPure tubes for which the traditional 1,000 µl tip is too short or too thick. Compatible with multi-channel pipette Finnpipette Novus 100 to 1,200 µl. 3 Graduations for visual control of pre-set pipetting volume at 200, 500 and 1,000 µl. - Empty box without pipette tips 95.1763, 8 pcs./case •

Colour code

* Expected September 2011

11

Tip SystemBox - Methodical tip processing The Tip SystemBox: Central Pipetting Station Simple and practical

Flexible and cost-effective Economic and environmentally friendly

Empty 96-tip refill trays and bulk tips

Suitable for manually racking bulk tips for autoclaving and convenient handling.*

Tip StackPack

Efficient handling of 96-tip trays, reduced packaging and storage space

Empty reload tray for use in the Tip SystemBox Users purchasing tips from our range loose in bags can load tips into empty trays (grey, yellow, orange) in the Tip SystemBox. •

Place empty trays into the Tip SystemBox and load with tips.

•

Autoclave tips in the Tip SystemBox, if required. See important notes on autoclaving on page 20.

Ordering Information empty reload trays Coloured trays for easy tip volume identification Order No.

Tip type** / Volume

Packaging (pcs./case)

95.1760.011

A, C

/ 200 µl

25

95.1760.022

D, J, N / 20 µl

25

95.1760.033

E long / 10 µl

25

95.1760.034

E

/ 10 µl

25

95.1760.044

L

/ 300 µl

20

* Important notes on autoclaving: See page 20 ** Pipette type see page 18 to 19

12

Tray colour code

Tip StackPack reload system Tips are preloaded in stacked trays of 96 tips each, stacked on top of each other. The base section and cover are designed for multiple use in combination with the 5 or 6 tip trays. As a result, composite materials and storage space are reduced by more than 50%. • Space-saving • Easy to use • Reduced waste volumes • Tip StackPack Starter Kit (3 x Tip StackPack + 1 Tip SystemBox) Ordering Information for Tip StackPack see page 19

Economic and environmentally friendly •

Easy transfer of individual layers of 96-tip trays into the Tip SystemBox for easy handling or autoclaving in the pipette tip box*

•

Safety labels applied on both sides can be easily perforated to access the next layer of tips

•

Trays can be placed into the Tip SystemBox without further adjustment

•

Tips can also be used directly from the stack

Safety labels applied on both sides can be easily perforated to access the next layer of 96 tips

Ordering Information Tip SystemBox Order No.

Description

Packaging pcs./case

95.1298.001

Tip SystemBox (without Pipette tips), empty box

6

Tip StackPack Starter Kit The Tip StackPack Starter Kit contains 3 Tip StackPacks and 1 Tip SystemBox each

Ordering Information Tip StackPack Starter Kit Order No.

Volume

Pipette tip colour

70.760.591

250 µl

neutral

70.760.592

200 µl

yellow

70.1130.690

10 µl

neutral

Colour code

Packaging/case 3 x Tip StackPack + 1 x Tip SystemBox

* Important notes on autoclaving: See page 20

13

Eppendorf Pipettes

✓

70.1131.210

✗ ✗ ✗ ✓✓✓

✗ ✗

✗

70.1115.210 70.1115.410 70.1114.210 70.1114.410

✗ ✓✓

✗ ✓

✓

70.1116.210

✓✓✓

✓✓

✓

70.760.213 70.760.413

NEW

NEW

10

70.760.212 70.760.412

✓ ✓✓ 20 20 ✓✓✓

✓ ✗ ✓ 20 20 ✗ ✓✓✓ ✗

✓✓ 20 ✓ ✗ ✓ ✗ ✓ ✓ 100

70.1189.215

✓✓✓

✓✓✓

✓ ✓ ✓

70.760.211 70.760.411

✓✓✓

✓ ✗

✓ ✗

70.765.210

✗ ✗ ✓

✗ ✗ ✓✓

✓✓

100

100

70.762.211 70.762.411

✓

✓

✓

70.1186.210 70.1186.410

✓

✓

✓ Eppendorf Research

10 µl

Eppendorf Research

10 µl

Eppendorf Reference

✓✓

70.1131 70.1131.100

✗ ✗ ✗

✗ ✗

✗

70.1114 70.1114.100 70.1114.200

✗ ✓✓

✗ ✓

✓

✓✓✓

✓

✓

✓✓✓

✓✓

✓

20 µl 20 µl

70.1116 70.1116.100 70.1116.200

200 µl

70.760.002 70.760.102 70.760.202 70.760.502

✓✓✓

✓✓✓ ✗

✓✓✓ ✗

70.1189.105

✓✓✓

✓✓✓

✓✓✓

✓✓✓

✓ ✗

✓ ✗

✗ ✗ ✓

✗ ✗ ✓✓

✗ ✗ ✓✓

250 µl 300 µl

70.765 70.765.100 70.765.200

1,000 µl

70.762 70.762.100 70.762.200

✓

✓

✓

70.1186 70.1186.100 70.1186.200

✓

✓

✓

5 ml

70.760.001 70.760.201 70.760.501

1,250 µl

200 µl

70.1115 70.1115.100 70.1115.200

NEW

100-1,000 µl

20-200 µl

30-300 µl MC

✓

10

✓✓✓

20 µl

2-20 µl

✓

70.1130 70.1130.100 70.1130.200 70.1130.600

NEW

10-100 µl

0.5-10 µl MC

1-10 ml

500-5,000 µl

100-1,000 µl

20-200 µl

30-300 µl MC

2-20 µl

0.5-10 µl

0.1-2.5 µl

50-200 µl

100-1,000 µl

10 µl

✗ ✓

10 µl

2-20 µl

2.5

10 µl

10-100 µl

2.5 µl

✓ ✓ 2.5

70.1130.210 70.1130.410

✓✓ ✓ 2.5 2.5 ✓✓

20 µl

70.1130.212

Quality Pipette Tips without filter* (illustrations 60%)

5 ml

Scale 1:2

10 ml

Scale 1:2

Scale 1:2

70.1183.001 70.1183.101

✗

70.1183.002 70.1183.102

✓

70.1187.001 70.1187.101 Legend:

✓ ✓

= max. pipetting volume

10 = limited pipetting volume

✗

14

Eppendorf Research

20 µl

2-20 µl

Eppendorf Research

1,250 µl 1,000 µl 300 µl 200 µl 200 µl 100 µl 20 µl

Biosphere®-Pipette Tips with filter (illustrations 60%)

0.5-10 µl

0.1-2.5 µl

Eppendorf Reference

10-100 µl

Compatibility Table - Pipette Tips

= not recommended

= not checked MC

= Multi-channel pipette

Thermo Finnpipettes

✓✓

✓

2.5 2.5

Gilson Pipettes

✓✓

✓✓ ✓ 2.5 2.5

✓ ✗ ✓ 2.5

✓ ✓

✓✓

✓✓ ✗

✓✓

✓

✗ ✗

✗

✗

✗ ✗

✗ ✗ ✗

✓✓✓

✓✓

✓ ✓

✓ ✓

✓✓

✗ ✗ ✗

✓✓

✓ ✓

✓ ✓ ✓

✓✓

✓✓

✓✓

✓ ✓

✓ ✓

✓✓

✓✓ ✗

✓✓

✓

20 20

✓✓ ✓

plus

✓

✗

✓✓ 20

✓✓ ✓ 100 100

✗

✗ ✓

20

✓

✓ ✓

✓✓

✗

✗ ✓

✓✓

✗

✗ ✓✓

200 200

✓

✓✓

200

✓

✓ ✓

✓✓

✓✓

✓

Finnpipette Digital / F1 / F2

Finnpipette Focus

✗ ✗ ✓✓

✓

✓ ✓

✓

Bio Finnpipette Novus Control

Pipetman / Ultra / Concept

✓✓

✓ ✓

✓✓

✓✓✓

✓✓ ✗

✗ ✗

✗

✗ ✗

✗ ✗ ✗

✓✓✓

✓✓

✓ ✓✓✓✓✓

✓✓

✓

✓ ✓

✓

✓✓

✓ ✓

✓✓

✗

✓

✗

✓✓ ✓ ✓ ✓ 200

✓

✓✓

✓✓

✗

✗ ✓

✗ ✗ ✓✓

✗

✗ ✓✓

250 250

✓✓

✓ ✓✓

✓

✓✓

U8 20-300 µl

✗ ✗ ✓ ✗

200 200

✓✓

Xplorer

P1000/U1000 200-1,000 µl P5,000/U5,000 1,000-5,000 µl P10/U10 1-10 ml

✓✓✓

✓ ✗

Eppendorf Research pro

P100/U100 20-100 µl P200/U200 20-200 µl

C8/C12 1-10 µl

2.5 2.5

P20/U20 2-20 µl

P2/U2 0.2-2 µl

Pipetman / Ultra / Concept P10/U10 0.1-10 µl

50-300 µl MC

100-1,200 µl MC

100-1,000 µl

30-300 µl

Bio Finnpipette Novus Control 30-300 µl MC

100-1,000 µl

1-10 µl

30-300 µl

0.5-5 µl

0.3-3 µl

200-1,000 µl

Finnpipette Focus 100-1,000 µl

50-300 µl MC

20-200 µl Univ.

5-50 µl MC

10-100 µl Univ.

2-20 µl Univ.

0.5-10 µl MC

F1 1-10 µl

0.5-10 µl Univ.

0.2-2 µl

Finnpipette Digital / F1 / F2 F2 0.5-5 µl

0.5-10 µl MC

100-5,000 µl

50-1,000 µl

50-1,200 µl MC

20-300 µl

20-300 µl MC

5-100 µl

5-100 µl MC

0.5-10 µl

0.5-10 µl MC

Eppendorf Research pro

1-10 ml

500-5,000 µl

plus

Xplorer

✓✓ ✗ ✗

✓✓

✓

200

✓✓✓ ✓

✗ ✗ ✓✓ 250

250

✓

✓✓

✓

✗ ✗ ✓✓

✓ ✗

✓✓

✓

✓ ✗

✓

✓✓

✓✓

✓

✓

✓

✗

✗

✓

✓

✓

✗

✓

✓ Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes.

* Important notes on autoclaving see page 20

15

Biohit Pipettes 0.1-1 µl

100-5,000 µl

50-1,000 µl

50-1,200 µl MC

0.2-10 µl

10-300 µl

100-5,000 µl

100-1,000 µl

50-300 µl MC

20-200 µl

50-250 µl MC

5-50 µl

0.5-10 µl

0.1-2.5 µl

100-5,000 µl

100-1,000 µl

20-200 µl

30-300 µl MC

2.5 2.5

✓✓

✓ ✓ 2.5

2.5

✓

✗

10 µl

2-20 µl

2.5 µl

70.1130.212 70.1130.210 70.1130.410

✓✓

✓

✓

✗

70.1131.210

✗ ✗

✗ ✗

✗

✗

70.1115.210 70.1115.410

✗

✗

✓

✗

✓

20 µl

70.1116.210

✓✓

70.760.213 70.760.413

20 µl

70.1114.210 70.1114.410

1,250 µl 1,000 µl 300 µl 200 µl 200 µl 100 µl 20 µl

NEW

70.760.212 70.760.412 NEW

✓✓ ✓✓ ✗ ✗ ✗ 20 20 ✓✗ ✗ ✓ ✓ 100

✗ ✗ ✗ ✗✓ 100

✓✓✓

70.1189.215

✓

✗ ✗

✓

70.760.211 70.760.411

✗✓

✓✓ ✗ ✗ ✓ 200 200

✗

70.765.210

✗ ✓✓

✗ ✗ ✓✓✓

✓

70.762.211 70.762.411

✓

✓

✓✗

70.1186.210 70.1186.410

✓

✓

✗

Quality Pipette Tips without filter* (illustrations 60%)

eLine Electronic

✓✓

✓✓

✓

✓

70.1131 70.1131.100

✗ ✗

✗ ✗

✗

✗

✗

✗

✓

✗

70.1115 70.1115.100 70.1115.200 70.1116 70.1116.100 70.1116.200 70.760.002 70.760.102 70.760.202 70.760.502

✓✓ ✗✓✗

✓✓ ✗ ✗ ✗ ✓

✓

70.760.001 70.760.201 70.760.501 70.765 70.765.100 70.765.200 70.762 70.762.100 70.762.200

✓

✓

✓✗

70.1186 70.1186.100 70.1186.200

✓

✓

✗

5 ml

250 µl

70.1189.105

✗

300 µl

NEW

✓✓

1,250 µl 1,000 µl

200 µl

20 µl

70.1114 70.1114.100 70.1114.200

20 µl

NEW

200 µl

20 µl

10 µl

Proline Mechanical

10 µl

mLine Mechanical 70.1130 70.1130.100 70.1130.200 70.1130.600

5 ml

Scale 1:2

10 ml

Scale 1:2

Scale 1:2

✗✓

✓ ✓ ✓ ✓ ✓ 250

✗

✗ ✓✓

✗ ✗ ✓✓✓

✓

70.1183.001 70.1183.101

✗

✗

✗

70.1183.002 70.1183.102

✓

✓

✓

70.1187.001 70.1187.101 Legend:

✓

= max. pipetting volume

10 = limited pipetting volume

✗

16

eLine Electronic

10 µl

10-100 µl

0.5-10 µl

Proline Mechanical

10 µl

Biosphere®-Pipette Tips with filter (illustrations 60%)

0.5-10 µl MC

mLine Mechanical

5-50 µl MC

Compatibility Table - Pipette Tips

= not recommended

= not checked MC

= Multi-channel pipette

✓✓

✓✓ ✗ 2.5 2.5

2.5 2.5 2.5 2.5

✓✓✓✓

✓ ✓ 2.5

2.5

✓

✓✗

2.5

✓✓ 10

✗ ✓✓

✓✓✓✓

✓✓

✓

✓✓

✓

✗ ✗ ✓✓

✗ ✗ ✗

✗ ✗

✗

✗ ✗

✗

✗ ✓✓

✗ ✗ ✗ ✗ ✓✓✓✓

✓✓

✗

✓✓

✗

✓✓

✗

✓✓

✗ ✓✓

2.5 2.5

10

10 10

✓✓

✓✓✓ 20 ✗ ✗

✓✓✓✓ ✓ ✓✓ 20 20 ✗ ✗ ✗✓✗

✗ ✗ ✗ ✗ ✗

✓

100

✗ ✓✓✓ ✗ 10 20 ✗ ✗ ✗✓

20

100

5-50 µl MC

✓

✓✓

✗ ✗ ✓

20-200 µl MC

Acura Multi 855/955e 0.5-10 µl MC

0.5-5 ml

10-200 µl

A. 835/ 935e 100-1,000 µl

5-50 µl

10-100 µl

2-20 µl

0.1-2 µl

20-200 µl

1-10 µl

10-100 µl

20-200 µl

100-1,000 µl

2-20 µl

10-100 µl

1-10 µl

1-10 µl

1-10 µl

Acura Manual Micro 825 Acura Electro Micro 925

Calibra Manual Micro 822 C. M. Multi 852 0.2-2 µl

20-200 µl

100-1,000 µl

2-20 µl

1-20 µl MC

0.5-10 µl MC

1-10 ml

0.5-10 µl

Transferpette Electronic 100-1,000 µl

20-200 µl

30-300 µl MC

2-20 µl

10-100 µl

0.5-10 µl MC

0.1-1 µl

0.5-10 µl

25-250 µl

100-1,000 µl

20-200 µl

Transferpette S 30-300 µl MC

5-50 µl

10-100 µl

2-20 µl

2-20 µl

0.5-10 µl

Transferpette Digital

Socorex Manual / Electronic Pipettes 0.5-10 µl

Brand Pipettes

✓✓ ✓ ✓✓✓ 20 20 ✗ ✗ ✗ ✓✓✓

✓✗ ✗✓

100

✓✗ ✓✓

100

100

✓✓✓✓ ✗ ✗ ✗ ✗ ✗

✗ ✗✓✗

✗ ✗ ✗ ✓✓

✗ ✗✓✗

✓

✗ ✗ ✗ ✗

✗ ✗

✗ ✗ ✗ ✗✓

✗✓

✓

✗ ✗ ✗✓

✗ ✗

✗ ✗ ✗ ✗✓

✗✓

100

✗✓

✓

✓

✓

✓

✗✓

✓

✓

✓

✓

Transferpette Digital

Transferpette S

Transferpette Electronic

Calibra Manual Micro 822 C. M. Multi 852

Acura Manual Micro 825 Acura Electro Micro 925

A. 835/ 935e

Acura Multi 855/955e

✓✓

✗ ✓✓

✓✓✓✓

✓✓

✓

✓✓

✓

✗ ✗

✗ ✗ ✗

✗ ✗ ✗ ✗

✗ ✗

✗

✗ ✗

✗

✓✓

✓✓

✓✓

✗ ✓✓

✓✓✓✓

✓✓

✗

✓✓

✓✓

✗ ✓✓

✓✓✓✓

✓✓

✗

✓✓

✓✓✓ ✗ ✗

✓✓✓ ✗

✓

✓✓✓✓

✓✓

✗

✓✓✓✓✓

✓✓

✓✓✓✓ ✓✓✓ ✗ ✗

✓✓✓ ✗

✓

✗ ✗ ✗✓

✗ ✗

✗ ✗ ✗ ✗✓

✗✓

✗ ✗ ✗ ✓✓

✗ ✗✓✗

✓

✗ ✗ ✗✓

✗ ✗

✗ ✗ ✓✓✓

✗✓

✓✓

✓

✓

✓

✓

✓✓

✗

✓

✓

✓ ✗ ✓

✓

✗

Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes. * Important notes on autoclaving see page 20

17

Ordering Information - Quality tips with filter Filter tips (illustrations 1:1)

Order No.

Max. Sample Quality Volume/ Grade Colour

Type E

Type E

70.1130.212

2.5 µl, neutral

96/box 480/inner box 1,920/case

70.1130.210

10 µl, neutral

96/box 480/inner box 1,920/case

70.1130.410

10 µl, neutral

480/bag (loose) 4,800/case

70.1131.210

10 µl, neutral

96/box 480/inner box 1,920/case

70.1115.210

10 µl, neutral

96/box 480/inner box 1,920/case

70.1115.410

10 µl, neutral

480/bag (loose) 4,800/case

70.1114.210

20 µl, neutral

96/box 480/inner box 1,920/case

70.1114.410

20 µl, neutral

480/bag (loose) 4,800/case

70.1116.210

20 µl, neutral

96/box 480/inner box 1,920/case

70.760.213

20 µl, yellow

96/box 480/inner box 1,920/case

70.760.413

20 µl, yellow

480/bag (loose) 4,800/case

70.760.212

100 µl, yellow

96/box 480/inner box 1,920/case

70.760.412

100 µl, yellow

480/bag (loose) 4,800/case

70.1189.215*

200 µl, neutral

96/box 960/case

70.760.211

200 µl, neutral

96/box 480/inner box 1,920/case

70.760.411

200 µl, neutral

480/bag (loose) 4,800/case

70.765.210

300 µl, neutral

96/box 480/inner box 1,920/case

70.762.211

1,000 µl, blue

100/box 500/inner box 1,000/case

70.762.411

1,000 µl, blue

200/bag (loose) 2,000/case

70.1186.210

1,250 µl, neutral

96/box 768/case

70.1186.410

1,250 µl, neutral

384/bag (loose) 3,840/case

Type E long

Type D

NEW

Type N

Type J

Type A

Type A

NEW

Type O extra long

Type C

Type L

Type B

Type M

* Expected September 2011

18

Packaging

Ordering Information - Quality tips without filter Tips without filter (illustrations 1:1)

Order No. Type E

Max. Sample Quality Volume/ Grade Colour

70.1130

1,000/bag, 10,000/case

70.1130.100 70.1130.200 70.1130.600

Type E long

Tip StackPack Starter Kit

70.1131

70.1115

70.1114 70.1114.100 70.1114.200

Type J

70.1116 70.1116.100 70.1116.200

Type A

10 µl, neutral

10 µl, neutral

20 µl, neutral

20 µl, neutral

70.760.002 70.760.202 70.760.502

Type O

500/bag, 10,000/case 96/box, 1,920/case 1,000/bag, 10,000/case 96/box, 1,920/case 96/box, 480/inner case, 1,920/case 1,000/bag, 10,000/case 96/box, 1,920/case 96/box, 480/inner case, 1,920/case 1,000/bag, 10,000/case 96/box, 1,920/case 96/box, 480/inner case, 1,920/case 500/bag, 10,000/case

70.760.102

NEW

96/box, 480/inner case, 1,920/case Tip StackPack

70.1130.690

70.1115.200

Type N

10 µl, neutral

96/tray, 576/StackPack, 2,304/case

70.1115.100

NEW

96/box, 1,920/case

70.1130.460

70.1131.100

Type D

Packaging

96/box, 1,920/case 200 µl, yellow

96/box, 480/inner case, 1,920/case Tip StackPack

70.760.452

96/tray, 480/StackPack, 1,920/case

70.760.592

Tip StackPack Starter Kit 200 µl, neutral

extra long

70.1189.105*

Type C

70.760.001

500/bag, 10,000/case

70.760.201

96/box, 480/inner case, 1,920/case Tip StackPack

70.760.501 70.760.451

250 µl, neutral

70.760.591

Type L

70.765 70.765.100 70.765.200

Type B

70.762 70.762.100 70.762.200

Type M

70.1186 70.1186.100 70.1186.200 70.1183.001

Scale 1:2

70.1183.101 70.1183.002

Scale 1:2

70.1183.102 70.1187.001

Scale 1:2

70.1187.101

96/box, 960/case

96/tray, 480/StackPack, 1,920/case

Tip StackPack Starter Kit 300 µl, neutral

1,000 µl, blue

1,250 µl, neutral

5 ml

5 ml

10 ml

500/bag, 5,000/case 96/box, 1,920/case 96/box, 480/inner case, 1,920/case 250/bag, 5,000/case 100/box, 1,000/case 100/box, 500/inner case, 1,000/case 384/bag, 3,840/case 96/box, 3,840/case 96/box, 768/case 250/bag, 2,500/case 50/Box, 500/case 250/bag, 2,500/case 50/box, 500/case 200/bag, 2,000/case 25/box, 250/case

* Expected September 2011 Refer to pp. 14 to 17 or see Catalogue for further information

19

Pipettenspitzen Biomek Pipettierroboter Pipette tips for für Biomek pipetting platforms

The quality solution concept for automated liquid handling •

Each production batch is tested for functionality and precision on original robotic instruments to ensure a consistently superior product quality

•

All tips are certified DNase- and human DNA-free

•

Sterile tips are also tested to be non-pyrogenic

• The coloured trays enable easy identification of tips and volumes

NEW: Pipette tips for Biomek 1000 · 2000 Colour code, tray

Product / Volume

Tip with filter, sterile

Tip without filter, non-sterile

Tip without filter, sterile

Packaging

70.1140.211*

70.1140.101**

70.1140.201**

96/rack *4,800/case **9,600/case

70.1141.211

70.1141.101

70.1141.201

96/rack 9,600/case

70.1142.211

70.1142.101

70.1142.201

96/rack 9,600/case

----

70.1143.101

70.1143.201

72/rack 3,600/case

Tip with filter, sterile

Tip without filter, non-sterile

Tip without filter, sterile

Packaging

70.1140.212*

70.1140.102*

70.1140.202**

96/rack *4,800/case **9,600/case

70.1141.212**

70.1141.102*

70.1141.202*

96/rack *4,800/case **9,600/case

70.1142.212*

70.1142.102*

70.1142.202**

96/rack *4,800/case **9,600/case

20 µl 20 µl

35 µl 50 µl

175 µl 250 µl

1,000 µl

NEW: Pipette tips for Biomek FX/NX · 3000 Colour code, tray

Product / Volume 20 µl 20 µl

35 µl 50 µl

175 µl 250 µl

Important notes on autoclaving: Products made from PP can be autoclaved up to 121°C without significantly impairing their mechanical properties. Users are to test for themselves if autoclaving may have any effect on other characteristic product features so as to influence the individual application concerned.

20

Reliable quality – Multiply® reaction tubes for PCR The original Multiply® tubes are the result of consistently improving the design of the more traditional reaction tubes. Enhanced design features of the Multiply® tubes ensure work is risk-free from contamination and non-tiring compared to traditional tubes.

• Integral anti-contamination shield:

Prevents accidental contact with the inner surface of the lid



Easy to open: Anti-contamination shield prevents potential contamination.

• Space-saving lid design:

Frosted, flat lid with large writing area



The frosted surface prevents smearing even when processed in a thermocycler. The space-saving lid design allows complete utilization of the heating block capacity.

• Interchangeable RackSystem for 0.2 ml tubes:

Sample preparation/storage in the RackSystem pipetting station



Small recess at the skirt of the Multiply® PCR tubes allows tubes to be easily removed from the work tray. The small, roughened recess can also be used as an additional writing area. Description for RackSystem see page 23

• Additional benefits of the Multiply® product range: • On request, we also offer conveniently packaged small quantities of products certified to the sterile Biosphere®, or ‘PCR Performance Tested’ quality standard • Variety of options • Optimum heat transfer thanks to thin-walled reaction space • Compatible on thermocyclers with 0.2 and 0.5 ml block formats • Enables micro centrifugation in most commonly used micro centrifuges (with adapter, as required)

21

Ordering Information – Multiply® PCR Tubes PCR single tubes Product

Order No.

Volume

72.735.100 72.735.002

Packaging

neutral

100 pieces

500 pcs./inner case 1,000 pcs./case

----

neutral

500 pieces

2,000 pcs./case

----

mixed colours ●●●●●●

500 pieces

3,000 pcs./case

Biosphere® sterile

neutral

250 pieces

1,000 pcs./inner case 2,000 pcs./case

----

neutral

500 pieces

2,000 pcs./case

----

mixed colours ●●●●●●

500 pieces

3,000 pcs./case

Description

Sterile

Colour

Pieces per bag

Packaging

Biosphere® Multiply®-µStripPro

Biosphere® sterile

neutral

50 strips

200 strips/inner case 400 strips/case

----

neutral

120 strips

480 strips/case

----

mixed colours ●●●●

120 strips

480 strips/case

----

neutral

120 strips

480 strips/case

----

mixed colours ●●●●

120 strips

480 strips/case

----

neutral

120 strips

1,200 strips/case

----

neutral

120 strips

480 strips/case

----

mixed colours ●●●●

120 strips

480 strips/case

Sterile

Colour

Biosphere® Multiply®-Pro 0.5

Biosphere® sterile

0.5 ml Multiply®-Pro 0.5

72.735.992 Biosphere® Multiply®-Pro 0.2

72.737 72.737.002

Quality Grade

Pieces per bag

Description

0.2 ml Multiply®-Pro 0.2

72.737.992

PCR tube strips with and without lid Product

Order No.

Volume

72.990 72.990.002 72.990.992

Multiply -µStripPro ®

72.991.002 72.991.992

0.2 ml

Quality Grade

NEW 72.991.103

Multiply®-µStrip Pro, low profile

72.985.002 Multiply®-µStrip 72.985.992

Strips of lid for Multiply®-µStrip and Multiply® PCR plate Product

Order No.

Description

compatible with

Sterile

Colour

Quality Grade

Strips per bag

Packaging

12 strips

240 strips/case

120 strips

480 strips/case

NEW 65.989

NEW 65.989.002

22

Multiply®-µStrip (72.985.002 / strips of 8 lids, 72.985.992) flat, and highly transparent, ® Multiply for real-time PCR PCR plate (72.985)

Biosphere® sterile

neutral

----

Accessories: Interchangeable RackSystem for 0.2 ml Multiply® PCR tubes • • • • •

Safe and fast handling of Multiply® tubes Flexible, two-piece system comprising a work tray and support base Easily inserted in the thermocycler, no need to remove tubes Convenient 96 well microtiter format to fit any processing system Stackable design provides space-saving specimen storage

Flexible, compact work station for sample preparation and space-saving specimen storage

Suitable for all Multiply® tubes

Sample preparation and PCR applications 1

2

3

4

5

6

Assembly

0.2 ml Multiply® tube loading...

...or, alternatively, Multiply® PCR plate loading.

Ice-bath pipetting

Specimen centrifugation in completely assembled RackSystem...

...fast and easy placement of specimens into the thermocycler.

Position empty PCR work station to rest securely on the support base.

Align single tubes to 45° for easy handling.

The PCR plate comprises 96 tubes in 12 strips of 8 tubes each.

RackSystem can be used as a pipetting station.

Compatible with any microtiter tray rotor.

After amplification, reposition PCR tray onto stackable support base for space-saving storage.

Ordering Information PCR RackSystem Illustration

Order No.

95.987.002

Product Description

PCR work tray for 0.2 ml PCR tubes

Description

Packaging

for 96 tubes

5/bag

95.988

neutral

95.988.001

red

●

blue

●

95.988.003

green

●

95.988.004

yellow ●

95.988.002

Support base for PCR work tray

5/case

23

C

D

4

3

E

F

G

5

6

7

8

9

10

H

11

12

NEW: White PCR plates for quantitative PCR

• 10-fold fluorescence level • Improved sensitivity • Enhanced reproducibility

Cost-effective miniaturisation of qPCR assay in white Multiply® PCR plates Fluorescence-based applications like qPCR, particularly those involving small volumes, benefit from the significantly improved reflection characteristics of the white wells. Specimens of low DNA concentration, too, can be more easily detected (see Fig. 1). Compared to transparent wells, a 10-fold fluorescence level can be measured with white wells. Thanks to maximum signal reflection, Ct values are obtained earlier (see Fig. 2). In white plates sealed with qPCR compatible tapes, the reagent volume (SYBR Green) per well can be reduced from 25µl to 15µl without significant changes in sensitivity and stability, enabling a 40% decrease in qPCR reagent costs. In addition, the white plates minimise adverse effects like “cross talk” that would increase well-to-well variability in transparent wells.

Ct values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes

Increased sensitivity due to maximum signal reflection

35 30 25

Fluorescence

B

Ct values

2

1

A

20 15 10 5

Ct at 15 µl (white) Ct at 25 µl (transparent)

0

1,000,000 Fig. 1

24

100,000

10,000

Number of plasmid copies

1,000

100 Fig. 2

Cycles

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

11

12

H

Multiply® PCR plates made from polypropylene, white • • • • •

White wells for improved fluorescence reflection Certified “PCR Performance Tested” Thin-walled reaction tubes for quick temperature transfer Fits thermocyclers processing 96 and 384 well block formats Barcode labeling on plates with half or full skirt is available on request

0.2 ml 96 Well Multiply® PCR plate, without skirt - - - - -

NEW: Easy to cut Black alphanumeric print for enhanced well identification Raised well rims for optimal sealing with heat-resistant tape Fits most commonly used thermocyclers Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1978.232

96 Well Multiply® PCR plate with half skirt, flat deck - - - - -

NEW

Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers Black alphanumeric print for enhanced well identification Flat deck enables safe sealing with tapes Raised well rims for optimal sealing with heat-resistant tape Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1979.132

96 Well Multiply® PCR plate with full skirt, “low profile” well design - - - - -

Enhanced stability for automated systems Suitable for real-time systems Black alphanumeric print for enhanced well identification Raised well rims for optimal sealing with heat-resistant tape Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1980.232

25

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

H

11

12

NEW: White PCR plates for quantitative PCR NEW: Lightcycler 480 PCR plate with 96 wells

NEW

- - -

Black alphanumeric print for enhanced well identification Raised well rims for optimal sealing with heat-resistant tape Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1982.202

NEW: Lightcycler 480 PCR plate with 384 wells

NEW

- - - -

Max. volume: 30 µl per well Black alphanumeric print for enhanced well identification Raised well rims for optimal sealing with heat-resistant tape Can be sealed with qPCR film 95.1999 (see p. 33)

72.1985.202

White Multiply® PCR plates and lid strips for 96 Well plates Product

Special feature

Description

72.1978.232

Optimised for qPCR

72.1979.132

Packaging

96 Well, without skirt

white

25 pcs./inner case 100 pcs./case

Optimised for qPCR

96 Well, with half skirt and low profile

white

25 pcs./inner case 100 pcs./case

72.1980.232

Optimised for qPCR

96 Well, with full skirt, low profile for Fast PCR

white

25 pcs./inner case 100 pcs./case

72.1981.232*

Optimised for qPCR

ABI style, 96 Well, with half skirt, low profile for Fast PCR

white

25 pcs./inner case 100 pcs./case

72.1982.202

Optimised for qPCR

Lightcycler 480, 96 Well, with half skirt

white

25 pcs./inner case 100 pcs./case

72.1985.202

Optimised for qPCR

Lightcycler 480, 384 Well, with full skirt

white

50 pcs./inner case 100 pcs./case

65.1998.002

Optimised for qPCR

strips of 8 lids, flat for 96 Well plates, highly transparent

transparent

480 pcs./case

NEW

NEW

NEW

* Available on request

26

Quality Grade

Colour

Order No.

Quality in series – 96 and 384 Well Multiply PCR plates ®

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

11

12

H

Multiply® PCR plates made from polypropylene • • • • •

Certified 'PCR Performance Tested' Quick temperature transfer due to thin-walled reaction tubes Fits thermocyclers processing 96 and 384 well block formats Raised well rims for improved sealing with heat-resistant tape Plates with half and full skirt; Barcode available on request

96 Well Multiply® PCR plate, without skirt, pre-assembled with strips of 8 tubes - - - - -

Available in the Biosphere® quality, individually wrapped sterile Suitable for real-time PCR Can be sealed with optically clear strips of 8 lids (order no. 65.989, see page 29) Tray from polycarbonate Suitable for use in RackSystem, see p. 23

72.985

0.2 ml 96 Well Multiply® PCR plate, without skirt - - - - - -

NEW: Easy to cut Black alphanumeric print for enhanced well identification Raised well rims for optimal sealing with heat-resistant tape Fits most commonly used thermocyclers Can be sealed with optically clear strips of 8 lids (see p. 29) Can be sealed with qPCR films (see pages 33 – 35)

72.1978.202

96 Well Multiply® PCR plate with half skirt - - - - - -

Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers Black alphanumeric print for enhanced well identification Suitable for real-time PCR Can be sealed with optically clear strips of 8 lids (see p. 29) Can be sealed with qPCR films (see pages 33 – 35) Also available with barcode label (72.1979.203)

72.1979.202

27

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

H

11

12

Quality in series – 96 and 384 Well Multiply® PCR plates NEW

NEW: 96 Well Multiply® PCR plate with half skirt, with flat deck - - - - - -

Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers Flat deck for easy and safe sealing of wells with tapes Black alphanumeric print for enhanced well identification Suitable for real-time PCR Can be sealed with optically clear strips of 8 lids (see p. 29) Can be sealed with qPCR films (see pages 33 – 35)

72.1979.102

96 Well Multiply® PCR plate with full skirt, 'low profile' well design - - - - -

Enhanced stability for automated systems Black alphanumeric print for enhanced well identification Suitable for real-time PCR Can be sealed with optically clear strips of 8 lids (see p. 29) Can be sealed with qPCR films (see pages 33 – 35)

72.1980.202

96 Well Multiply® PCR plate with half skirt, optimised for ABI Fast PCR System - - - - -

‘Low profile’ design reduces dead volume and cycle times Suitable for real-time systems Black alphanumeric print for enhanced well identification Can be sealed with optically clear strips of 8 lids (see p. 29) Can be sealed with qPCR films (see pages 33 – 35)

72.1981.202

384 Well Multiply® PCR plate with robust skirt - - - -

72.1984.202

28

40 µl maximum volume per well 8 apertures in the frame facilitate positioning and removal of the plate from the heating block Black alphanumeric print for enhanced well identification Can be sealed with qPCR films (see pages 33 – 35)

Ordering Information – Multiply PCR plates & lid strips ®

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

11

12

H

Multiply® PCR plates, transparent Product

Quality Grade

Well/Max. Volume

Description

Sterile

Colour

96 / 0.3 ml

Biosphere® Multiply® PCR plate without skirt, pre-assembled, raised wells

Biosphere® sterile

neutral

1 pcs./bag 20 pcs./case

96 / 0.3 ml

Multiply® PCR plate without skirt

----

neutral

25 pcs./inner case 100 pcs./case

72.1979.102

96 / 0.3 ml

Multiply® PCR plate, with half skirt and flat deck

----

neutral

25 pcs./inner case 100 pcs./case

72.1979.202

96 / 0.3 ml

Multiply® PCR plate with half skirt

----

neutral

25 pcs./inner case 100 pcs./case

Multiply® PCR plate with half skirt

----

neutral

25 pcs./inner case 100 pcs./case

Order No.

72.985

72.1978.202

96 / 0.3 ml

Packaging

72.1979.203

with barcode label

72.1980.202

96 / 0.2 ml

Multiply® PCR plate with skirt 'low profile'

----

neutral

25 pcs./inner case 100 pcs./case

72.1981.202

96 / 0.2 ml

Multiply® Fast PCR plate 'low profile'

----

neutral

25 pcs./inner case 100 pcs./case

72.1984.202

384 / 40 µl

Multiply® PCR plate with skirt

----

neutral

50 pcs./bag 50 pcs./case

Lid strips for Multiply®-µStrip and Multiply® PCR plate Product

Order No.

Description

compatible with

Sterile

strips of 8 lids, flat, highly transparent, for real-time PCR

Multiply® PCR plate 72.985

Biosphere® sterile

Colour

Quality Grade

Strips per bag

Packaging

12 strips

240 strips/case

120 strips

480 strips/case

NEW 65.989

Multiply®

65.1998.002

strips of 8 lids, flat, highly transparent, for real-time PCR

PCR plates 72.1978.202, 72.1979.102, 72.1979.202, 72.1980.202, 72.1981.202

neutral

----

29

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

H

11

12

Multiply® PCR plates - Compatibility Table PCR plate range - Compatibility Table 96 Well 96 Well without skirt, without skirt pre-assembled

Model

96 Well with half skirt, high profile

96 Well with full skirt, low profile

96 Well with half skirt, Fast PCR, low profile

384 Well with full skirt

72.985

72.1978.202 72.1978.232

72.1979.202 72.1979.203 72.1979.102 72.1979.132

72.1980.202 72.1980.232

72.1981.202 72.1981.232

72.1984.202

MegaBace 1000 DNA Analysis System (old Model)

✗

✗

✗

✓

✗

✗

MegaBace 1000 DNA Analysis System (old Model)

✗

✗

✗

✓

✗

✗

MegaBace 4000 DNA Analysis System

✗

✗

✗

✗

✗

✓

MegaBace 5000 DNA Analysis System

✗

✗

✗

✓

✗

✓

✓

✓

ABI Prism 7000, 7700

✓

✓

ABI Prism 7300, 7500, 7700 real-time PCR-System

✗

✓

ABI Prism 7900HT real-time PCR-System

✗

✓

ABI Prism 7500 Fast real-time PCR-System

✗

✗

✓

ABI Prism 7900 Fast real-time PCR-System

✗

✗

✓

ABI Prism 7900HT Fast real-time PCR-System

✗

✗

✓

ABI Prism 9800 Fast PCR-System

✗

✗

✓

Order No. Standard PCR plates Amersham Bioscience

Applied Biosystems ABI 2720

NEW

✓

Gene Amp 9600

✓

✓

✓

Gene Amp 9700

✓

✓

✓

Gene Amp 7500/5700

✓

✓

PE 2700

✓

✓

✓

✓

✓

✓

✓

✓

✗

✗

Seq. ABI Prism 310 Genetic Analyzer

✓

✓

✗

✓*

Seq. ABI Prism 3100/3130 Genetic Analyzer

✓

✓

✗

✓*

✓

✓

✗

✓*

PE 9600 PE 9700 StepOnePlus Fast real-time PCR-System

NEW

Seq. ABI 3700/3730/3730 XL DNA Analyzers Veriti 96 Well / 384 Well

NEW NEW

✓

✓

✓

✓

✗

✗

T1

✓

✓

T3

✓

✓

T Gradient

✓

✓

TRobot

✓

✓

✓

Uno

✓

✓

✓

Veriti Fast 96 Well

✓

✗

✓

✗

Biometra

Uno II

* with compatible adapter from ABI

30

✓ ✓

✓

✓

2

1

A

B

C

D

4

3

E

F

G

5

6

7

8

9

10

11

12

H

PCR plate range - Compatibility Table Model

96 Well without skirt, preassembled

96 Well without skirt

Order No. Standard PCR plates

72.985

72.1978.202 72.1978.232

96 Well 96 Well Lightcycler 96 Well with half 384 Well with half 480, with full skirt, skirt, with full skirt, 96 Well, low profile Fast PCR, skirt high profile full skirt low profile

Lightcycler 480, 384 Well, full skirt

72.1979.202 72.1979.203 72.1979.102 72.1979.132

72.1985.202

72.1980.202 72.1980.232

72.1981.202 72.1982.202 72.1984.202 72.1981.232

Bio-Rad/MJ Research

✓

CFX 96, real-time

✓ ✓ ✓

iCycler, real-time iQ5, real-time MyCycler Opticon 2 System, real-time / C1000 Serie / S1000 Serie

✓ ✓ ✓

PTC-200 PTC-225/220/221 PTC-100

✓ ✓ ✓ ✗ ✓ ✓ ✓

✗ ✗ ✗ ✓ ✓ ✓ ✓

✓ ✓ ✓ ✓

✓ ✓

Corbet Research

✓

Palm Cycler

Eppendorf

✓ ✓

✓ ✓ ✓ ✗ ✗ ✗

Mastercycler ep realplex

✓ ✓

Mastercycler Gradient Mastercycler pro

✓ ✓

✓

Ericom Deltacycler SingleBlock TwinBlock

✓ ✓ ✓

✓ ✓ ✓

✗ ✗ ✗

✓

✓

✓

MWGA Primus 96

✓

Primus 384 THEQ

✓

The Q-Lifecycler

✓

✓

Roche NEW

Lightcycler 480

✓

✓

Stratagene Gradient Temp. Cycler MX3000/4000 Multiplex, QPCR System

✓

✓ ✓ ✓

Robocycler 96/384

Techne

✓ ✓ ✓ ✓

✓ ✓ ✓ ✓ ✗ ✓

✓

✓

Flexigene Genius OMN-E

✓

PCR Express Primus 96 Px2/PxE Quantica, real-time TC412/TC512 Touchgene Gradient

Legend:

✓ ✗

✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓

✓ ✓ ✓ ✓ ✓

✓ ✓

✓

= recommended = not recommended = not checked

31

96 Deep well MegaBlock® 1.2 / 2.2 ml

MegaBlock® 96 Well The MegaBlock® 96 Well is ideal for automated processing of sample volumes from 1.2 to 2.2 ml or handling large quantities of backup samples. • Sealing foils/mats available • Products are manufactured under clean room conditions and protected against contamination • Free from human DNA, DNase/RNase, endotoxins

MegaBlock® 1.2/2.2 ml, PP

MegaBlock® 1.2 ml, PS transparent

• • • • •

• • • • •

Each well 100% leak-proof tested Ideal for backup storage of pharmaceutical substances, DNA isolation, long-term sample storage, and enzyme and tissue culture applications Solvent-resistant to DMSO Square cavities(2.2 ml) with round bottom wells with alphanumeric coordination system Autoclavable*

Each well 100% leak-proof tested Ideal for long-term storage of blood samples Pierceable mat cover Transparent for viewing Round bottom wells with alphanumeric coordination system

MegaBlock® Order No.

Description

Volume/Material

82.1970.002

round, raised wells

82.1971.002

round, raised wells

1.2 ml

PP

32 pcs./case

82.1972.002

square wells

2.2 ml

PP

32 pcs./case

1.2 ml

PS

transparent

Packaging 32 pcs./case

Lids and tapes for MegaBlock® Order No.

Description

Packaging

95.1990.002

Cover for 1.2 ml MegaBlock®, pierceable, suitable for 82.1970.002 and 82.1971.002

250 pcs./ case; 50 pcs./ inner case; 10 pcs./bag

95.1991.002

Cover for 2.2 ml MegaBlock®, suitable for 82.1972.002

250 pcs./ case; 50 pcs./ inner case; 10 pcs./bag

Aluminium foil, pierceable

100 foils/ inner case

95.1995 82.1586

Acetate film, transparent

100 foils/ inner case

NEW

95.1992

Adhesive tape, 96-fold perforation, pierceable

100 foils/ inner case

NEW

95.1993

Strongly adhesive tape, transparent

100 foils/ inner case

Refer to pp. 34 – 35 for a detailed description of 95.1995, 95.1992, 95.1993 * Important notes on autoclaving on p. 20

32

Adhesive sealing tapes for qPCR/PCR, automation and sample storage

Adhesive sealing tapes for qPCR Hermetical sealing of micro test plates made from polypropylene, polystyrene and polycarbonate to exclude evaporation and protect samples during applications, storage and transport requires tailor-made tape materials. The Sarstedt range includes a variety of sealing tapes specially designed to meet the sophisticated requirements of PCR, storage of active substances, and High Throughput Screening. All tapes are produced under clean room conditions to exclude contamination with DNase/ RNase and nucleic acids.

Highly transparent adhesive tape for quantitative PCR The 50 µm polyolefin film is coated with a smear-free, crystal clear adhesive that shows minimal adhesion at room temperature and is, therefore, exceptionally easy to handle. Thanks to the initially low stickiness, the film on the PCR plate can be easily shifted to the correct position, if necessary. The strong adhesive effect is brought about during PCR and as a result of the heat produced by the cover, minimising loss by evaporation. • Highly transparent film ideally suited for real-time PCR and other fluorescence-based applications

NEW

• Excellent sealing properties with minimised evaporation values due to innovative adhesive • No distortion under thermal strain / prevents cross contamination • Does not stick to skin or gloves during application • Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol • Functional sealing range: -70°C to 110°C

Ordering Information Order No.

Description

Packaging

95.1999

Highly transparent, suited for qPCR with pressure sensitive adhesive

100 tapes/inner case

33

Adhesive sealing tapes for qPCR/PCR, automation and sample storage Transparent adhesive tape for end point PCR and sample storage, strongly adhesive

NEW

The tape is made from a 50 µm polyester film coated with a special adhesive for immediate adhesive effect. • Efficient anti-evaporation protection due to strongly adhesive effect • Easily removable due to firm “end tabs” • Compatible with aqueous solutions and organic solvents • Functional sealing temperature range: -40° to 110°C

Ordering Information Order No.

Description

Packaing

95.1993

transparent

100 tapes/inner case

Transparent adhesive tape for end point PCR and qPCR The optically transparent foil is made from a 50 µm polyolefin film with perforated “end tabs” for easy removal. • Optically transparent foil for standard and real-time PCR • Removable without leaving any residue and ideally suited for sample storage to -70°C • Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol • Functional sealing temperature range for PCR: up to 110°C

Ordering Information Order No.

Description

Packaging

95.1994

transparent

100 tapes/inner case

Aluminium foil for PCR and sample storage The heat-resistant, robust, pierceable 38 µm aluminium foil stands out for efficient antievaporation protection and resistance to solvents. Perforated “end tabs” can be easily removed after attaching the foil. • Tape can be pierced with probes and pipette tips • Removable without leaving any residue • Ideally suited for storing sample material/compounds to -70°C • Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol • Functional sealing temperature range for PCR: up to 110°C

Ordering Information

34

Order No.

Description

Packaging

95.1995

aluminium

100 tapes/inner case

Pierceable adhesive tape for DNA sequencers, HPLC and autosamplers The 50 µm polyester film coated with a strong adhesive has 96 perforated openings for easy piercing. The perforated openings are free from adhesive to prevent samples, needles or pipette tips from sticking to the tape.

NEW

• Easy sampling with tips or pipetting systems • Temporary protection against evaporation and ideal for samples in 96 well plates • Resealable for long-term protection with a second tape (e.g. 95.1993) • Compatible with aqueous solutions and organic solvents • Cost-effective tape for use in DNA sequencers, HPLC and autosamplers • Functional sealing temperature range -40°C to 110°C

Ordering Information Order No.

Description

Packaging

95.1992

96 fold perforated, transparent

100 tapes/inner case

Recommended tape options Order No.

Product description

NEW

Application

Special features

Pierceable

Material

Functional temperature range

96-fold perforated cover tape

Processing/DNAsequencer, HPLC, autosampling

Easy to pierce, 96 perforated opening w/o adhesive

yes

Polyester

-40°C to 110°C

95.1993

Transparent PCR tape, strongly adhesive

PCR, sample storage

Strongly adhesive, eliminates edges, fringing

no

Polyester

-40°C to 110°C

95.1994

Transparent PCR and qPCR tape

PCR, real-time PCR

Good optical transparency, high chemical resistance

no

Polyolefin

-70°C to 110°C

95.1995

Adhesive aluminium foil

Sample storage

Pierceable light protection for samples, high chemical resistance

yes

Aluminium

-70°C to 110°C

Adhesive, optically highly transparent qPCR tape

real-time PCR, fluorescence analyses

Highly transparent, pressure-sensitive adhesive, minimal evaporation rates

no

Polyolefin

-70°C to 110°C

95.1992 NEW

NEW

95.1999

35

SafeSeal micro tubes with push cap

0.5 / 1.5 and 2.0 ml Micro Tubes with sterile Biosphere® purity are routinely used when RNase-, DNA-, ATP-free and non-pyrogenic conditions are essential. Superior to standard reaction tubes, Biosphere® Micro Tubes have been developed and continually enhanced to suit the requirements in medical and molecular-biological research:

Secure SafeSeal cap locking The 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tube features a closing mechanism which prevents unintentional popping open under thermal stress. • Wide hinge for easy and precise closure • Large writing space on lid • Centrifugation up to 30,000 x g (2.0 ml Micro Tubes up to 25,000 x g)

36

Minimizing the risk of contamination To reduce storage time in open bags and inherent contamination risks, Biosphere® SafeSeal Micro Tubes are packaged 50 pieces per bag. For most stringent demands in sample preparation, individually wrapped SafeSeal tubes in Biosphere® quality are available. Optionally available non-sterile 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tubes in ‘PCR Performance Tested’ quality: DNase-, RNase-, human DNA- and PCR inhibitor-free.

NEW

Fig.: SafeSeal Micro Tube individually wrapped

Ordering Information – Micro Tubes with SafeSeal push cap

Volume Description

sterile · non-pyrogenic · DNA-free RNase-free · ATP-free

0.5 ml

1.5 ml

2.0 ml

Order No.

Packaging

Order No.

Packaging

Order No.

Packaging

72.704.400

250/bag 500/inner case 2,000/case

72.706.400

250/bag 1,000/inner case 2,000/case

72.695.400

250/bag 1,000/inner case 2,000/case

72.704.200

50/bag 250/inner case 500/case

72.706.200

50/bag 250/inner case 500/case

72.695.200

50/bag 250/inner case 500/case

72.704.201

individually wrapped 50/inner case 100/case

72.706.201

individually wrapped 60/inner case 120/case

72.695.201

individually wrapped 60/inner case 120/case

37

UV-Transparent Disposable Cuvettes for photometric analyses in the UV range ≥ 220 nm The plastic material of the half micro cuvette enables measurements in the UV range from wave lengths of 220 nm. Contamination caused by the repeated rinsing processes required for quartz glass cuvettes can be avoided by using the UV-transparent disposable cuvette. Apart from non-certified UV cuvettes, our range includes a certified option individually sealed with a cap and packed in a Styrofoam container to protect from scratches.

Typical applications and product features •

Photometric analysis of nucleic acids at 260 nm and proteins at 280 nm

•

Low UV absorbance for DNA, RNA and protein quantification

•

Compatible with photometers of 8.5 mm and 15 mm light centre height: Eppendorf Biophotometer, ThermoSpectronic, Perkin Elmer, Bio-Rad, Analytica Jena etc.

•

Minimizes absorbance deviations due to consistently accurate optical quality

•

Economical low-cost alternative to quartz cuvettes

•

Certified UV cuvettes (DNA-/RNase-/Protein-free) individually sealed with caps optionally available for safe sample storage and prevention of contamination

For measurements in the short-wave UV range below 300 nm, the material-related self-absorptive characteristics of PS and acrylic (PMMA) cuvettes increase distinctively as their transmission ration decreases.

Wavelength in nm

nm

Transmission in % UV cuvette

Acrylic cuvette

Polystyrene cuvette

260

73

23

0

280

86

68

0

313

94

93

66

334

96

97

79

366

97

98

90

405

98

99

95

560

98

99

96

Transmission values in individual cuvettes UV cuvette Acrylic cuvette Polystyrene cuvette

38

Technical specification Min. sample volume:

50 µl

Optical path length:

10 mm

Basic absorbance:

≤ 0.134 A at 260 nm; ≤ 0.065 A at 280 nm

External dimensions (W x D x H): 12.5 x 12.5 x 45 mm Light centre height (LCH):

8.5 mm and 15 mm

Ordering Information UV Cuvettes Order No.

Length

LCH

Packaging

67.758

45

8.5 mm

100 pcs. in Styrofoam box; 400 pcs./case

67.758.001

45

8.5 mm

100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case certified DNA-/RNase-/Protein-free

67.759

45

15 mm

100 pcs. in Styrofoam box; 400 pcs./case

67.759.001

45

15 mm

100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case certified DNA-/RNase-/Protein-free

39

Technical modifications reserved This document may contain information on products that may not be available in particular countries 362g-200-0211-0911

Please call if you require more information!

SARSTEDT AG & Co. P.O. Box 12 20 D-51582 Nümbrecht Phone +49 2293 3050 Fax +49 2293 305- 122 [email protected] www.sarstedt.com