Merial Avian Forum 2014 

“Newcastle disease: control in  different field challenges”.

Francisco Perozo DVM., MS., PhD. University of Zulia, Venezuela. Paris, France April 23 ‐ 25

For this talk… • Newcastle disease a worldwide problem for the Poultry  Industry. • Newcastle disease Variants???? ‐‐ Important facts… • Solutions…. ‐ Key factors in the control.  ‐ What to do depends on where you are… • Vaccination  ‐ New technologies. ‐ VG/GA strain AVINEW alternative programs.

Why NDV? Because is the main concern for endemic countries…. still a threat for the NDV Free industry worldwide

16 weeks.… fallen birds, nervous signs, high mortality

NEWCASTLE DISEASE DISTRIBUTION 2013

America    2013

Southeast Asia 2013

Other countries 2013…..

Epidemiology and Prevalence  •Mandatory report to the OIE. •Worldwide distribution. •Free countries still in risk due to  globalization and international trade. •Prevalence data supported by Molecular  epidemiology. 

Question Are there NDV variant strains?

NO

Protectotype

Criteria to claim a variant is serology and cross protection



There are GENOTYPE DIFFERENCES that reflect in the virus behaviour, but not in the antigenic arrangements or serology.

03CNUNK 06CNGSE 98CNFWL 55 01CN096PAR 01CNGSE 63 72 99CN097PEN 02CNGSE 89 00CNGSE 100 00CNGSE 100 97CNGSE 03CNUNK 01CNGSE 05CNMLD 100 96 100 03CNCKN 94 06CNGSE 100 98CNCKN 97CNGSE 100 00TWFWL 100 00CNCKN 100 100 03CNUNK 90 02CNUNK 00CNFWL 92 100 96CNFWL 100 90IDCCT 75IRPGN 72USFON 97CNFWL 90KECKN 99ARPGN 87 99 100 84USPGN 84UKPGN 100 98CNPGN 94 100 96CNPGN 87 98CNGSE 97ARPGN 93 98BEPGN 100 100 00ITDVE 00ITCKN 100 00USPGN 100 04USPGN 100 04USPGN 100 05USPGN 93 73 04USPGN 100 64

Complete coding region of the fusion gene

100

59

95

VII

VI

Class II

NDV genotyping 62

92USTKY

93USANH 71USMXD

58

96

05MXCKN 06MXCKN 04MXDVE 00HNCKN 93 96MXCKN 100 98MXCKN4 100 02USGFL 100 00MXCKN (Torreon) 92 00MXCKN10 100 01MXCKN 97CNUNK 33UKHRT 06CNUNK 00ARSWN 100 98ARDCK 88 89USNOP 53 86USMLD 74 45USBEA 46USLAS 100 47USB1 100 01RUDCK 100 100 02RUBKT 87 04USMLD 67UKULS 01USRKN 59 99 02USRDT 01AUUNK 100 70 66AUQV4 76JPDCK 99 02CNCKN 100 04USRDT 02USMLD 100 02USMLD 82 98USGWT 94 01USDCK 68 00USRKN 93 03USMLD 71 01USMLD 72 99USMLD 86 01USMLD 100 77100

40

44

46

V

III/IV II I

Class I

VENEZUELA

II 96

100

VII

VI

V

IV

100

86

11527326-Chicken(B1)/USA/47 70

0.02

148534707-Goose/China/06 268043733|Goose/China /08 Chicken /Venezuela/08 227460816|-Duck China /02 22657972- Goose/China/02 259221332 –Duck/China/2008 18042226-Goose (ZJ1)/China/2007 76 28933797|Goose(China/2007| 100 146359113-Mallard/China/06 111559170|- Chicken/Korea/08 100 157674506- Chicken/Korea/08 157674520-Chicken (recombinant)/Korea/08 80 109290353-Chicken/China/02 94429033- GM strain/China/07. 97 109290360-Chicken/China/2003 88 91 109290367-Chicken/China/2003 99 110225441- Goose/NA-1/China/06 100 -161110504-Chicken (SRZ)/ China/03| 45511204-Cockatoo/Indonesia/90. 100 33772453-Chicken /USA/72 45511225-Chicken USA/72 33772468- Chicken/Kenya/90 100 269838478-Pigeon(1.3)/UK/07| 100 269838473-Pigeon(0.025)/UK/07 100 258547239 Pigeon(AV324)Netherland/96. 87298942- Pigeon/Italy/82. |217323230-Pigeon (Maryland)/USA/84. 100 99 217323219-Pigeon(New York)/USA/84 100 33772440|Mixed/USA/71 87 45511239-Mixed (Largo)/USA/71 33772474-Chicken/Mexico/96 253317820-Cormorant/USA/92 100 100 |253317827-Cormorant/USA/97| 100 253317778-Cormorant/USA/97 253317785-Cormorant/canada/98 100 253317792-Cormorant/Canada/95 86 76 |253317799-Cormorant/Canada/95 53636432-Chicken (Herts)/UK/33 293933-Chicken/Australia/32 293927-Chicken(LaSota)/USA/46 100

7229463-Chicken(QV4)Australia/67 61393384-Chicken/Australia/98 100 61393396-Chicken/Australia/92 100

I

III

• Genotypes V, VI , VII and VIII are the predominant genotypes circulating worldwide. • Genotype V South and Central America (1970s) •Genotype VI emerged in Asia in the 60’s and remained until Genotype VII took over 20 years ago… Genotype VIII is also present in Asia.

Do homologous genotype vaccines protect better?

Avian Dis. 2012 Sep;56(3):464-70. Characterization of live LaSota vaccine strain-induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype. Cornax I1, Miller PJ, Afonso CL.

“Birds vaccinated an adequateoftiter of LaSota Properly done with vaccination healthy birds (Genotype II) strain live vaccine are fully protected from with current genotype commercial vaccines morbidity and mortality due toIIchallenge with (Genotype will deem adequate protection against any VII) ZJ1 strain NDV” genotype present in the field.

• Compared homologous versus heterologous genotype vaccination against a genotype VII and VIII  lethal challenge  • No difference in clinical protection was observed,  despite the challenge virus,  LaSota protected 100%  • A decrease in viral shedding was observed in the  homologous genotype groups when compared with  LaSota group.

• Compared homologous versus heterologous genotype  vaccination against a genotype V and VII lethal challenge  • No difference in clinical protection despite the challenge  virus, LaSota protected 100%  • All vaccines decreased viral shedding. It was slightly  lower (not P