HUPO Australia/New Zealand Chromosome 7 Initiative Ed Nice Monash University, Melbourne, Australia and Sichuan University, Chengdu, China

[email protected]

Key Aims To effectively populate the Chr 7 database using mass spectrometry

and

antibody

data

derived

from

participating groups and the literature. In particular, the

deposition of data has been strongly encouraged, and it is proposed that all human MS data be deposited into ProteomeXchange and a PXD quoted on all NHMRC funded work.

Current data in NeXtProt

Missing Proteins Strategy Using an ”in silico” approach to search alternative

biological databases to provide clues to guide MS and antibody-based approaches: other species, the literature,

patent databases

Using this approach strong evidence on the existence of a number of missing proteins has been generated.

To

assist

in

the

tracking

of

MissingProteinPedia is being developed.

these

data,

a

Missing ProteinPedia (MPP) MMP is a community knowledgebase addressing the Human Proteome Project’s PE2-4 proteins MMP is open, comprehensive, communal, unedited, unannotated, unbiased, evolving, non-judgemental, evidence-based, transparent, global, searchable and sortable (by chromosome, tissue and keywords) MPP aims to capture the broadest level of scientific data necessary to increase the rate at which we validate PE2-4 proteins. MPP represents a new community-based proteomics tool, analogous to human genome annotation jamborees MMP will bring biological clues to assist in the validation of missing proteins

Funding This continues to mainly come indirectly from NHMRC and ARC grants. Interactions between related C-HPP and B/DHPP projects are proving effective.

This is where data is being generated

This is the catalogue KB should be widened to include other biological data sources

Communication To broaden the awareness of HUPO we have attempted to

reach beyond the proteomics community to our biochemical and clinical colleagues. This has been achieved by: Word of mouth Mailouts

Promotion at key meetings: Lorne Proteomics, Protein, Cancer, Australian Peptide Conference Invited presentations at a number of Universities and Institutes Communication

with

other

relevant

societies:

Australian College of Pathologists, APS, PUG, PMV

A directory of Australian capability is being generated

EACR,

Workshops and Meetings Inaugural C-HPP Human Proteome Missing Proteins Workshop, July 2014, Macquarie University, Sydney A one day meeting entitled “The Omics Revolution: Uncovering the Complexity of the Human Proteome,” sponsored by the HUPO 2010 Association was held in Kingscliff, Australia, in Sep 2015 as a satellite to the 11th Australian Peptide Conference. The meeting comprised four sessions: The Proteomics Toolbox Biomarker Discovery and Validation New Applications Emerging Trends in Proteomics.

Sponsored by HUPO 2010

International HUPO Collaborations Generation of highly validated monoclonal antibodies to key proteins. In collaboration with Dr. Emma Lundberg and Prof Mathias Uhlen, panels of monoclonal antibodies against a number of key proteins including important signaling molecules, proteins not currently represented in the Protein Atlas, and missing proteins have been generated and validated. prESTs, developed for the Human Protein Atlas, which have been used as immunogens allowing direct comparison between the corresponding monoclonal and polyclonal antibodies. The Unified Human Protein Browser. The groups in Australia and China have continued to work together on the generation of the UHPB presented in Madrid and Segovia. Biobanking. Mark Baker (Macquarie) has collaborated with Gyorgy Marko Varga (Lund University) to develop high-level biobanking relationships with ISBER and European biobank equivalents. Chromosome 7/17 EGF/EGFR collaboration

Take Home Messages • It is important to maximise interactions between the BD and C-HPPs • It is important to bring biological validation into HUPO • It is important to reach out to the global scientific community

"The Omics Revolution: Towards Personalised Medicine" Official satellite to the 2016 Australian International Peptide Conference Peppers Noosa Resort Queensland, Australia 15th to 19th October 2017

HUPO Australia/New Zealand Chromosome 7 Initiative