Safety Data Sheet (SDS/MSDS)

(According to regulation (EC) 1907/2006.) Date SDS established : 12 / 2015

Protease

Revision number : 02 // ASAY-PRT-0656

1. IDENTIFICATION OF THE SUBSTANCE / MIXTURE AND OF THE COMPANY / UNDERTAKING 1.1 PRODUCT IDENTIFIER Product Name: Proteinase K Catalog #: RFIT-SUB-0317, RFIT-SUB-0318, RFIT-SUB-0389 Kit Components: Protease (Single Vial), Protease (30 pack), Protease (6 pack) 1.2 RELEVANT IDENTIFIED USES OF THE SUBSTANCE OR MIXTURE AND USES ADVICES AGAINST Enzyme for nucleic acid modification 1.3 DETAILS OF THE SUPPLIER OF THE SAFETY DATA SHEET Address: BioFire Defense, LLC 79 W 4500 S Suite 14, Salt Lake City, Utah 84107, USA Telephone Number: 1-801-262-3592 E-mail Address: [email protected]

1.4 EMERGENCY TELEPHONE NUMBER Call your local emergency center. 2. HAZARDOUS IDENTIFICATION 2.1 CLASSIFICATION OF THE MIXTURE Skin irritant (Category 2) Protease Skin Sensitizer (Category 1)

Eye irritant (Category 2) Respiratory Sensitizer (Category 1)

2.2 LABEL ELEMENTS Labeling according Regulation (EC) No 1272/2008 [CLP] Pictogram/Signal Word: Hazardous Statements H315 H317 H319 H334 H335 Precautionary Statements P280 P302+P352

Danger Causes skin irritation May cause an allergic skin reaction Causes serious eye irritation May cause allergy or asthma symptoms or breathing difficulties if inhaled May cause respiratory irritation Wear protective gloves/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water

P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing

P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental Hazard Statements None 2.3 OTHER HAZARDS None Page 1 of 7

3. COMPOSITION/INFORMATION ON INGREDIENTS Component Name/ EC nr. Cas# Classification Concentration Hazardous Ingredient Proteainase, Xi;R36/37/38 tritirachium album 254-457-8 39450-01-6 2.5% R42/43 serine 4. FIRST AID MEASURES 4.1 DESCRIPTION OF FIRST AID MEASURES Move to fresh air. If not breathing, give artificial respiration. If breathing is If Inhaled: difficult, give oxygen. off immediately with soap and plenty of water, removing all In Case of Skin Contact: Wash contaminated clothes and shoes thoroughly with plenty of water for at least 14 minutes and consult a In Case of Eye Contact: Rinse physician. mouth with water, and afterwards drink plenty of water. Never give In case of Ingestion Clean anything by mouth to an unconscious person. Notes fo Physician Treat symptomatically 5. FIREFIGHTING MEASURES 5.1 EXTINGUISHING MEDIA Flammable Properties: Flash point:

Not flammable Not determined

Suitable Extinguishing Dry chemical, CO2, water spray or regular foam Media: Unsuitable Extinguishing Media: None Hazardous combustion No information available products Sensitivity to Mechanical Impact: Not sensitive Explosion Data: Sensitivity to Static Discharge: Not Sensistive Protective Equipment As in any fire, wear self-contained breathing apparatus pressure-demand, and Precautions for MSHA/NIOSH (approved or equivalent) and full protective gear. Firefighters

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6. ACCIDENTAL RELEASE MEASURES 6.1 PERSONAL PRECAUTIONS, PROTECTIVE EQUIPMENT, EMERGENCY PROCEDURES Avoid contact with skin, eyes and clothing. Use personal protective equipment. Ensure adequate ventilation. 6.2 METHODS AND MATERIAL FOR CONTAINMENT AND CLEANING UP Prevent further leakage or spillage if safe to do so. Avoid dust formation. Pick up and transfer to properly labeled containers. Ventilate area and wash spill site after material pickup is complete. 7. HANDLING AND STORAGE 7.1 PRECAUTIONS FOR SAFE HANDLING Handle in accordance with good industrial hygiene and safety practice. 7.2 CONDITIONS FOR SAFE STORAGE, INCLUDING ANY INCOMPATIBILITIES Keep containers tightly closed in a dry, cool and well-ventilated place. No information available about incompatibilities. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Chemical Name

ACGIH TLV

Proteainase, tritirachium album N/A serine 39450-01-6 8.1 ENGINEERING MEASURES Showers, Eyewash stations, Ventilation systems

OSHA PEL

NIOSH IDLH

N/A

N/A

8.2 PERSONAL PROTECTIVE EQUIPMENT Eye/Face Protection: Safety glasses with side-shields Skin and Body Protection: Wear protective gloves/clothing. If exposure limits are exceeded or irritation is experienced, NIOSH/MSHA approved respiratory protection should be worn. Positive-pressure supplied air Respiratory Protection: respirators may be required for high airborne contaminant concentrations. Respiratory protection must be provided in accordance with current local regulations. Hygiene Measures: Handle in accordance with good industrial hygiene and safety practice. 9. PHYSICAL AND CHEMICAL PROPERTIES 9.1 INFORMATION ON BASIC PHYSICAL AND CHEMICAL PROPERTIES Appearance White Odor Slight Odor Threshold Decomposition Temperature ºC Freezing Point Initial Boiling Point

No information available No information available No information available No information available

Physical State Freeze-Dried pellet pH No data available Page 3 of 7

Flash point Not determined Autoignition Temperature No data available Boiling Point/Range No data available Melting Point/Range No data available Flammability Limits in Air No data available Explosive properties No information available Oxidizing properties No information available Evaporation Rate No data available MMHG @ 37.8 C No data available Vapor Density No data available Specific Gravity No data available Solubility No information available Water solubility Very soluble in water Partition Coefficient No data available (n-octanol/water) Viscosity No information available 10. STABILITY AND REACTIVITY 10.1 REACTIVITY No data available 10.2 CHEMICAL STABILITY Stable under recommended storage conditions 10.3 CONDITIONS TO AVOID No data available 10.4 HAZARDOUS DECOMPOSITION PRODUCTS No data available 10.5 HAZARDOUS POLYMERIZATION Hazardous polymerization does not occur 11. TOXICOLOGICAL INFORMATION 11.1 INFORMATION ON TOXICOLOGICAL EFFECTS Protease Irritating to eye, skin and respiratory system. Sensitizer. Acute Toxicity: Chronic toxicity: No known effect based on information supplied Target Organ Effects: Lungs 12. ECOLOGICAL INFORMATION 12.1 ECOTOXICITY The environmental impact of this product has not been fully investigated.

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13. DISPOSAL CONSIDERATIONS 13.1 WASTE TREATMENT METHODS Waste disposal method: Dispose of material in accordance with all federal, state, and local regulations Contaminated Packaging: Dispose of in accordance with all federal, state and local regulations 14. TRANSPORT INFORMATION No restrictions apply due to the low volumes. 15. REGULATORY INFORMATION REACH Title VII Restrictions: No information available Chemial Dangerous Organic Harmful Name Substances Solvents Substances Whose Names are to be Indicated on the Label Proteinase, Not Not Not tritirachium applicable applicable applicable album serine Component

Proteinase, tritirachium album serine 39450-01-6 (95100)

ISHA- Harmful substances Prohibited for Manufacturing, Importing, Transferring, or Supplying

ISHA- Harmful Substances Requiring Permission

Not applicable

Not applicable

Pollution release and transfer registry (Class II)

Pollution Release and Transfer Registry (Class I)

Poison and Deleterious Substances Control Law

Not applicable

Not applicable

Not applicable

Toxic Chemical Toxic Release Toxic Release Classification Inventory (TRI)- Inventory (TRI)Listing (TCCL)- Group I Group II Toxic Chemicals

Not applicable

International Inventories: TSCA Does not comply EINECS/ELINCS Complies ENCS Does not comply IECSC Complies KECL Does not comply PICCS Does not comply AICS Does not comply

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Not applicable

Not applicable

US Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). The product contains a chemical or chemicals which are subject to the reporting requirements of the Act and Title 40 of the Code of Federal Regulations, Part 372. SARA 311/312 Hazard Categories: Acute Health Hazard Yes Chronic Health Hazard No Fire Hazard No Sudden Release of Pressure Hazard No Reactive Hazard No Clean Water Act This product does not contain any substances regulated as pollutants pursuant to the Clean Water Act (40 CFR 122.21 and 40 CFR 122.42) Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) This product does not contain any substances regulated as hazardous air pollutants (HAPS) under Section 112 of the Clear Air Act Amendments of 1990. CERCLA US State Regulations California Proposition 65 This product does not contain any proposition 65 chemicals. International Regulations Mexico- Grade No information available Canada

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16. OTHER INFORMATION Disclaimer: Not for food, drug, household, agricultural or cosmetic use. The above information is correct to the best of our knowledge. The user should make independent decisions regarding completeness of the information based on all sources available. BioFire Defense shall not be held liable for any damage resulting from handling or from contact with the above product. It remains the user’s own responsibility to make sure that the information is appropriate and complete for his specific use of this product. The user is also responsible for observing any laws and applicable guidelines. Changes to previous version of the SDS: Added single vial protease and updated part numbers.

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Revision date: December 2015