Laboratory Aspects of Neuropsychiatric Disorders in Lyme and Coinfections

Laboratory Aspects of Neuropsychiatric Disorders in Lyme and Coinfections Conference of the German Lyme Disease Society in Wuppertal/Germany 9th Apr. ...
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Laboratory Aspects of Neuropsychiatric Disorders in Lyme and Coinfections Conference of the German Lyme Disease Society in Wuppertal/Germany 9th Apr. 2011 Armin Schwarzbach M.D. Ph.D. Specialist for laboratory medicine Member of the German Borreliosis Society (Deutsche Borreliose-Gesellschaft e.v.) Chairman of the laboratory test and international committee of the International Lyme and Associated Diseases Society (ILADS) USA Infectolab Laboratory for Lyme disease and coinfections Tel. +49 (821) 455471-0 www.b-c-a.de Speech / presentation German Lyme Disease Society, Wuppertal/Germany, Apr. 9h, 2010 This document is intellectual property of Armin Schwarzbach MD PhD; reproduction only with permission. Please note the copyright.

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Borrelia burgdorferi – The chameleon of symptoms and laboratory

Speech / presentation German Lyme Disease Society, Wuppertal/Germany, Apr. 9h, 2010 This document is intellectual property of Armin Schwarzbach MD PhD; reproduction only with permission. Please note the copyright.

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Aims of the immune-competent cells

CD57+NK-cells - Lysis antigen-antibodycomplexes (Borrelia)

NK

TH1

TH2

TCell

Elispot-LTT: - Borrelia - Chlamydia - Anaplasma Antibodies: - Borrelia - Chlamydia, Mycoplasma - Anaplasma, Babesia …

Speech / presentation German Lyme Disease Society, Wuppertal/Germany, Apr. 9h, 2010 This document is intellectual property of Armin Schwarzbach MD PhD; reproduction only with permission. Please note the copyright.

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Test sensitivity for Borrelia antibodies by ELISA screening: New data

Test producer

Aeskulap

Virion

Diasorin

Euroimmun

n=

patients

50

50

50

44

ELISA

negative +

4%

4%

14%

2%

Immunoblot

positive

ELISA

negative +

14%

12%

14%

16%

Immunoblot

borderline

18%

16%

28%

18%

42%

44%

32%

42%

Total

Sensitivity

(-40%)

Speech / presentation German Lyme Disease Society, Wuppertal/Germany, Apr. 9h, 2010 This document is intellectual property of Armin Schwarzbach MD PhD; reproduction only with permission. Please note the copyright.

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Laboratory example from practice: Negative EIA / Positive immunoblot B-C-A GmbH & Co. KG · Morellstraße 33 · 86159 Augsburg

Laboratory results Patient: Date of birth: 08/09/1947 Date of testing: 07/08/2009

Antibodies (Humoral immune system) Results Reference Borrelia burgdorferi-IgG-EIA 2.8 RU/ml

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