DIPARTIMENTO DI MEDICINA MOLECOLARE CURRICULUM DIDATTICO-SCIENTIFICO DEL PROF. PAOLO BIANCO

DATI PERSONALI Nome e Cognome

PAOLO BIANCO

Dipartimento

Dipartimento di Medicina Molecolare

Indirizzo

Viale Regina Elena 324, 00161 Roma Telefono uff./lab./mobile

+39 06 446 1972 (segreteria)

FAX

+39 06 494 0896

e-mail

[email protected]

POSIZIONE ATTUALE 2000- ad oggi

Professore Ordinario, Anatomia e Istologia Patologica

FORMAZIONE E TRAINING 1979

Laurea in Medicina e Chirurgia, cum laude, Sapienza Universita’ di Roma

1982 Roma

Specializzazione in Anatomia e Istologia Patologica, cum laude, Sapienza Universita’ di

1982-83 Visiting Fellow (CNR fellowship) Department of Anatomy and Developmental Biology, University College London 1988-89 Bethesda MD

Fogarty Visiting Associate, Bone Research Branch, NIDR, National Institutes of Health,

1998

National Institutes of Health Credentials, Anatomic Pathology

POSIZIONI PRECEDENTI 1984-92

Ricercatore, Anatomia e Istologia Patologica, Sapienza Universita’ di Roma

1992-2000

Professore Associato, Anatomia e Istologia Patologica, Universita’ dell’Aquila

1998-2007 Bethesda MD

Adjunct Senior Investigator (intermittent), CSDB, NIDCR, National Institutes of Health,

Curriculum Vitae

Pagina 1

2003-11

Lab Chief, Parco Biomedico Roma San Raffaele

INSEGNAMENTO – INCARICHI ATTUALI Coordinatore, Anatomia e Istologia Patologica, CLM in Medicina e Chirurgia A, Sapienza Universita’ di Roma Coordinatore, Dottorato di Ricerca in Fisiopatologia e Clinica dei tessuti Scheletrici, Sapienza Universita’ di Roma Patologia Molecolare, Corso di Laurea in Biotecnologie Mediche, Sapienza universita’ di Roma Medicina Rigenerativa, Corso di Laurea in Biotecnologie Mediche, Sapienza universita’ di Roma Diagnostica Istopatologica, Scuola di Specializzazione in Anatomia e Istologia Patologica, Sapienza Universita’ di Roma Anatomia Patologica, Corso di Laurea in Techico di Laboratorio Biomedico, Sapienza Universita’ di Roma

ATTIVITA’ CLINICA 20041998-2003 1984-87 1987-1992

Direttore, UOC Anatomia e Istologia Patologica B, Policlinico Umberto I, Roma Consultant, National Cancer Institute Laboratory of Pathology and The Warren Magnuson Clinical Center, National Institutes of Health, Bethesda MD Assistente Anatomo-Patologo, Policlinico Umberto I, Roma Consultant, Chake Chake Hospital, Pemba, Tanzania Aiuto Anatomo-Patologo, Policlinico Umberto I, Roma

ATTIVITA’ SCIENTIFICA Interessi scientifici

Cellule staminali e medicina rigenerativa; fisiopatologia dello scheletro; patologia molecolare; istopatologia investigativa e diagnostica; mouse genetics; modelli transgenici di malattia; modelli di malattia in cellule staminali

Finanziamenti

Telethon 1999-04 Principal Investigator Fibrous dysplasia of Bone. 2005-08 Principal Investigator. Fibrous Dysplasia of Bone Models of Disease, Models of intervention. 2009- Principal Investigator. Fibrous Dysplasia of Bone. Transgenic models of disease and models of therapy. AIRC

Curriculum Vitae

Pagina 2

2002-05 Principal Investigator. Cancer homing to bone: modeling the interaction of human prostate cancer cells with human marrow stromal cells. 2005-08 Principal Investigator. Mechanisms of bone metastasis: Modelling and manipulating the "seed and soil" interaction. European Union FP6 2004-07 GENOSTEM International Consortium. Executive Committee member, Unit PI. “Adult mesenchymal stem cells engineering for connective tissue disorders. From the bench to the bed side”. European Union FP7 2008-12 European Consortium ANGIOSCAFF. Unit PI. Ministero della Salute 2001 Cellule staminali emangioblastiche: fenotipo,espansione in vitro e ricostruzione tissutale. Unit PI. 2002-03 Programma Nazionale Cellule Staminali. Project Coordinator, PI, Unit PI. The bone marrow as the source of different sem cells: the microvascular connection and therapeutic potential. Unit funds 2009-10 Strategies for tissue repair. Unit PI. MIUR - PRIN 2001-02. Project Coordinator, PI, Unit PI. Advanced Models of human skeletal diseases. 2003-04 Project Coordinator, PI, Unit PI. Strategies of cell therapy for mesodermal tissues. 2006-07 Project Coordinator, PI, Unit PI. Generation and reversion of skeletal organogenic and pathogenic processes using stem cells. MIUR - FIRB 2003-06 Skeletal stem cells: nature, properties and use for the creation of models and therapies in genetic diseases of the skeleton. Principal Investigator. 2002-06 Tissue engineering for connective tissue repair with special reference to bone. Unit PI. 2006-10 Italy-Israel International cooperation. Project coodinator, PI, Unit PI. Generation and assay of “smart” cell material constructs for bone repair. 2007-12 National Research Network TISSUENET. Unit PI. Sapienza University of Rome 2001-09 “Ateneo” and “Facolta’” projects Fondazione Pasteur Cenci-Bolognetti

Curriculum Vitae

Pagina 3

2010-

Premi Mineral Society Member

PI, Gsa and the hematopoietic niche

1989 American Society for Bone and Mineral Research; 1998 International Bone and

Faculty of 1000 (F1000) Medicine, Rheumatology

Editorial Boards

Journal of Histochemistry and Cytochemistry, 1990-94, Journal of Bone and Mineral Research 2004-present, Journal of Tissue Engineering and Regenerative Medicine 2006-present;

Reviewer (sel)

Nature Medicine, Journal of Cell Biology, PlosOne, Journal of Biological Chemistry, Development, Journal of Cell Science, Journal of Cellular Physiology, Bioessays, Experimental Cell Research, FEBS Journal, Cell Death & Differentiation, Journal of Histochemistry and Cytochemistry, American Journal of Pathology, Diagnostic Molecular Pathology, Cell and Tissue Research, Journal of Cellular and Molecular Medicine, Journal of Medical Genetics, American Journal of Medical Genetics, Journal of Gene Medicine, Human Gene Therapy, Blood, Stem Cells and Development, Stem cells, Regenerative Medicine, Experimental Hematology, Tissue Engineering, Journal of Hematotherapy and Stem Cell Research, Journal of Clinical Endocrinology and Metabolism, Endocrinology, Clinical Endocrinology, Journal of Endocrinological Investigation, Journal of Bone and Mineral Research, Bone, Calcified Tissue International, Osteoporosis International, Archives of Oral Biology, Arthritis Research and Therapy, Arthritis and Rheumatism, Clinical Orthopedics and RR, European Journal of Oral Science, Brazilian Journal of Biological and Medical Research, Journal of Vascular Research, Biotechnology & Applied Biochemistry, Journal of Biomedical Materials Research, New Zealand Veterinary Journal, Pharmacology and Toxicology, Basic and Clinical Pharmacology and Toxicology, Biomacromolecules, Nanoletters, Cytotherapy, and others

Reviewer, Agenzie di Finanziamento National Institutes of Health, American Society for Bone and Mineral Research, John Simon Guggenheim Memorial Foundation, NY, NY, Institut National de la Sante et de la Recherche Medicale (INSERM), Ministère de la Recherche, Association Française contre les Myopathies, Juvenile Diabetes Research Foundation International, Agence Nationale de la Recherche (ARS), Agence Evaluation recherché Scientifique (AERES), The Wellcome Trust, Research into Ageing Charity UK, Hong Kong Scientific Council, Dutch Ministry of Health, Science Foundation of Ireland, Israel Science Foundation

Scientific Advisory Boards e review panels (sel.) Scientific Advisory Board, Dept of Biomedicine, University of Basel CH 2008-2012; International Advisory Board, Dept of Endocrinology, Odense University Hospital, DK 2010; Scientific Advisory Board, Center for Regenerative Medicine Galway,

Curriculum Vitae

Pagina 4

Irelad Sceince Foundation and the University of Galway, Ireland, 2008 Brevetti

US Patent 5,914,121 Formation of human bone in vivo using ceramic powders and human marrow stromal fibroblasts. Robey PG, Kuznetsov SA, Krebsbach P, Rowe DA, Bianco P, Mankani MH.

Invited Speaker (recenti, selezione) 2007

European Society of Pathology, Istanbul, Turkey (declined), Osteology Society, Vienna, Austria, Mesenchymal Stem Cells and Regenerative Medicine, Cleveland OH, Gordon Research Conference, Bones and Teeth, Biddeford NH, Mesenchymal Stem Cells, Montpellier, France, German Stem Cell Society, Wurzburg, Germany

2008

Stem Cells in Rome, Accademia dei Lincei, Rome; Gordon Research Conference, Craniofacial Morphogenesis and Tissue regeneration, Barga; Gordon Research Conference, Biomineralization, Meriden NH (declined); International Society for Cell Therapy, Antwerp, The Netherlands (declined); NIH Research Symposium Challenges and Promise of Cell-based Therapies, Bethesda MD; European Hematology Association, Mandelieu, France; Meibom Meeting, Wurzburg, Germany; International Workshop on Biomaterials for tissue engineering and BiotechnologicalApplications, Kharagpur, India (declined); Clinical Cell Analysis, Bremen, Germany; Hematopoietic Microenvironments, Paris, France

2009

International Bone and Mineral Society, Sydney, Australia; International Society for Stem Cell Research, Barcelona, Spain, Invited Lecturer, Stem Cell Center, Katholieke Universitat Leuven, Bone Research Society, London UK, UK Mesenchymal Stem Cell Meeting, Sheffield, UK, Invited Lecturer, Virchow Klinikum, Charite Berlin, Stem Cells and Bioengineering, Rostock, Germany, European Blood and Marrow Transplantation, Goteborg, European Hematology Association, Berlin, Accademia Nazionale dei Lincei, Rome, Bone Research Society – Extracellular Matrix Society Joint Meeting, London UK, ECTS Course, Oxford UK, International Meeting Phosphodiesterases and cAMP, National Institutes of Health Bethesda, MD, Gordon Research Conference, Cell and Molecular Biology of Bones and Teeth, Meriden NH, American Society for Bone and Mineral Research, Denver CO, Invited Lecturer, Stem Cell Center, Lund Sweden, 22nd IGB Meeting Stem Cells Molecular Physiology & Therapeutic Applications, Capri, Italy, 5th International Conference on Mesenchymal Stem Cells, Austin TX;

2010

Plenary Lecture, St Justine University Hospital Montreal; European Calcified Tissue Society, Glasgow; European Society for Pediatric Endocrinology, Stockholm; 10th Intl Conference Cancer-induced Bone Disease, Sheffield UK; 1st Int. Meeting on Fibrous Dysplasia and McCune-Albright Syndrome, National Institutes of Health, Bethesda; Bone Biology Symposium, Dusseldorf; 8th Frontiers of Science International Symposium, Saitama, Japan;

2011

International Osteoporosis Foundation, Valencia; Advances in Stem Cell Research, Pasteur Institute, Paris; !st Int Conference on Dental and Craniofacial Stem Cells, Columbia University and the WTC, NY, NY; American Society for Bone and Mineral Research, San Diego CA; 7th European Summer School Stem Cells and Regenerative

Curriculum Vitae

Pagina 5

Medicine, Hydra, Greece; Mesenchymal Stem Cells – From Discovery to Translational Medicine, Chengdu, China; Stem Cell Symposium, University of Sao Paulo, Brazil Bibliografia, citazioni 148 papers in peer-reviewed journals; 35 invited chapters, intern. editions. ~8,000 citations; H-index 47 (ISI, Settembre 2011). URL

www.bianco-lab.it

PUBBLICAZIONI selezionate (ultimi 15 anni) A. Peer reviewed: (max 30 su un totale di: 148) 1.

Riminucci, M., L.W. Fisher, A. Shenker, A.M. Spiegel, P. Bianco, and P. Gehron Robey, Fibrous dysplasia of bone in the McCune-Albright syndrome: abnormalities in bone formation. Am J Pathol, 1997. 151(6): p. 1587-600.

2.

Bianco, P., S.A. Kuznetsov, M. Riminucci, L.W. Fisher, A.M. Spiegel, and P.G. Robey, Reproduction of human fibrous dysplasia of bone in immunocompromised mice by transplanted mosaics of normal and Gsalpha-mutated skeletal progenitor cells. J Clin Invest, 1998. 101(8): p. 1737-44.

3.

Riminucci, M., J.N. Bradbeer, A. Corsi, C. Gentili, F. Descalzi, R. Cancedda, and P. Bianco, Vis-a-vis cells and the priming of bone formation. J Bone Miner Res, 1998. 13(12): p. 1852-61.

4.

Xu, T., P. Bianco, L.W. Fisher, G. Longenecker, E. Smith, S. Goldstein, J. Bonadio, A. Boskey, A.M. Heegaard, B. Sommer, K. Satomura, P. Dominguez, C. Zhao, A.B. Kulkarni, P.G. Robey, and M.F. Young, Targeted disruption of the biglycan gene leads to an osteoporosislike phenotype in mice. Nat Genet, 1998. 20(1): p. 78-82.

5.

Holmbeck, K., P. Bianco, J. Caterina, S. Yamada, M. Kromer, S.A. Kuznetsov, M. Mankani, P.G. Robey, A.R. Poole, I. Pidoux, J.M. Ward, and H. Birkedal-Hansen, MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover. Cell, 1999. 99(1): p. 81-92.

6.

Riminucci, M., L.W. Fisher, A. Majolagbe, A. Corsi, R. Lala, C. De Sanctis, P.G. Robey, and P. Bianco, A novel GNAS1 mutation, R201G, in McCune-albright syndrome. J Bone Miner Res, 1999. 14(11): p. 1987-9.

Curriculum Vitae

Pagina 6

7.

Riminucci, M., B. Liu, A. Corsi, A. Shenker, A.M. Spiegel, P.G. Robey, and P. Bianco, The histopathology of fibrous dysplasia of bone in patients with activating mutations of the Gs alpha gene: site-specific patterns and recurrent histological hallmarks. J Pathol, 1999. 187(2): p. 249-58.

8. Bianco, P. and P. Gehron Robey, Marrow stromal stem cells. J Clin Invest, 2000. 105(12): p. 1663-8. 9.

Bianco, P., M. Riminucci, A. Majolagbe, S.A. Kuznetsov, M.T. Collins, M.H. Mankani, A. Corsi, H.G. Bone, S. Wientroub, A.M. Spiegel, L.W. Fisher, and P.G. Robey, Mutations of the GNAS1 gene, stromal cell dysfunction, and osteomalacic changes in non-McCune-Albright fibrous dysplasia of bone. J Bone Miner Res, 2000. 15(1): p. 120-8.

10.

Bianco, P., M. Riminucci, S. Gronthos, and P.G. Robey, Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells, 2001. 19(3): p. 180-92.

11. 21.

Bianco, P. and P.G. Robey, Stem cells in tissue engineering. Nature, 2001. 414(6859): p. 118-

12.

Jimenez, M.J., M. Balbin, J. Alvarez, T. Komori, P. Bianco, K. Holmbeck, H. Birkedal-Hansen, J.M. Lopez, and C. Lopez-Otin, A regulatory cascade involving retinoic acid, Cbfa1, and matrix metalloproteinases is coupled to the development of a process of perichondrial invasion and osteogenic differentiation during bone formation. J Cell Biol, 2001. 155(7): p. 1333-44.

13.

Kuznetsov, S.A., M.H. Mankani, S. Gronthos, K. Satomura, P. Bianco, and P.G. Robey, Circulating skeletal stem cells. J Cell Biol, 2001. 153(5): p. 1133-40.

14.

Riminucci, M., M.T. Collins, A. Corsi, A. Boyde, M.D. Murphey, S. Wientroub, S.A. Kuznetsov, N. Cherman, P.G. Robey, and P. Bianco, Gnathodiaphyseal dysplasia: a syndrome of fibro-osseous lesions of jawbones, bone fragility, and long bone bowing. J Bone Miner Res, 2001. 16(9): p. 1710-8.

15.

Minasi, M.G., M. Riminucci, L. De Angelis, U. Borello, B. Berarducci, A. Innocenzi, A. Caprioli, D. Sirabella, M. Baiocchi, R. De Maria, R. Boratto, T. Jaffredo, V. Broccoli, P. Bianco, and G. Cossu, The meso-angioblast: a multipotent, self-renewing cell that originates from the dorsal aorta and differentiates into most mesodermal tissues. Development, 2002. 129(11): p. 2773-83.

16.

Cattelino, A., S. Liebner, R. Gallini, A. Zanetti, G. Balconi, A. Corsi, P. Bianco, H. Wolburg, R. Moore, B. Oreda, R. Kemler, and E. Dejana, The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol, 2003. 162(6): p. 1111-22.

17.

Cossu, G. and P. Bianco, Mesoangioblasts--vascular progenitors for extravascular mesodermal tissues. Curr Opin Genet Dev, 2003. 13(5): p. 537-42.

18.

Holmbeck, K., P. Bianco, and H. Birkedal-Hansen, MT1-mmp: a collagenase essential for tumor cell invasive growth. Cancer Cell, 2003. 4(2): p. 83-4.

Curriculum Vitae

Pagina 7

19.

Holmbeck, K., P. Bianco, K. Chrysovergis, S. Yamada, and H. Birkedal-Hansen, MT1-MMPdependent, apoptotic remodeling of unmineralized cartilage: a critical process in skeletal growth. J Cell Biol, 2003. 163(3): p. 661-71.

20.

Riminucci, M., M.T. Collins, N.S. Fedarko, N. Cherman, A. Corsi, K.E. White, S. Waguespack, A. Gupta, T. Hannon, M.J. Econs, P. Bianco, and P. Gehron Robey, FGF-23 in fibrous dysplasia of bone and its relationship to renal phosphate wasting. J Clin Invest, 2003. 112(5): p. 683-92.

21.

Kuznetsov, S.A., M. Riminucci, N. Ziran, T.W. Tsutsui, A. Corsi, L. Calvi, H.M. Kronenberg, E. Schipani, P.G. Robey, and P. Bianco, The interplay of osteogenesis and hematopoiesis: expression of a constitutively active PTH/PTHrP receptor in osteogenic cells perturbs the establishment of hematopoiesis in bone and of skeletal stem cells in the bone marrow. J Cell Biol, 2004. 167(6): p. 1113-22.

22.

Dellavalle, A., M. Sampaolesi, R. Tonlorenzi, E. Tagliafico, B. Sacchetti, L. Perani, A. Innocenzi, B.G. Galvez, G. Messina, R. Morosetti, S. Li, M. Belicchi, G. Peretti, J.S. Chamberlain, W.E. Wright, Y. Torrente, S. Ferrari, P. Bianco, and G. Cossu, Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. Nat Cell Biol, 2007. 9(3): p. 255-67.

23.

Heegaard, A.M., A. Corsi, C.C. Danielsen, K.L. Nielsen, H.L. Jorgensen, M. Riminucci, M.F. Young, and P. Bianco, Biglycan deficiency causes spontaneous aortic dissection and rupture in mice. Circulation, 2007. 115(21): p. 2731-8.

24.

Michienzi, S., N. Cherman, K. Holmbeck, A. Funari, M.T. Collins, P. Bianco, P.G. Robey, and M. Riminucci, GNAS transcripts in skeletal progenitors: evidence for random asymmetric allelic expression of Gs alpha. Hum Mol Genet, 2007. 16(16): p. 1921-30.

25.

Mosig, R.A., O. Dowling, A. DiFeo, M.C. Ramirez, I.C. Parker, E. Abe, J. Diouri, A.A. Aqeel, J.D. Wylie, S.A. Oblander, J. Madri, P. Bianco, S.S. Apte, M. Zaidi, S.B. Doty, R.J. Majeska, M.B. Schaffler, and J.A. Martignetti, Loss of MMP-2 disrupts skeletal and craniofacial development and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth. Hum Mol Genet, 2007. 16(9): p. 1113-23.

26.

Sacchetti, B., A. Funari, S. Michienzi, S. Di Cesare, S. Piersanti, I. Saggio, E. Tagliafico, S. Ferrari, P.G. Robey, M. Riminucci, and P. Bianco, Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell, 2007. 131(2): p. 32436.

27.

Bianco, P., P.G. Robey, and P.J. Simmons, Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell, 2008. 2(4): p. 313-9.

28.

Piersanti, S., C. Remoli, I. Saggio, A. Funari, S. Michienzi, B. Sacchetti, P.G. Robey, M. Riminucci, and P. Bianco, Transfer, analysis, and reversion of the fibrous dysplasia cellular phenotype in human skeletal progenitors. J Bone Miner Res, 2010. 25(5): p. 1103-16.

Curriculum Vitae

Pagina 8

29.

Bianco, P., The stem cell next door: skeletal and hematopoietic stem cell "niches" in bone. Endocrinology, 2011. 152(8): p. 2957-62.

30. Bianco, P., Bone and the hematopoietic niche: a tale of two stem cells. Blood, 2011. 117(20): p. 5281-8.

CAPITOLI DI LIBRI (max 5 sel. su un totale di: 35) Bianco P, Riminucci M: The bone marrow stroma in vivo: ontogeny, structure, cellular composition and changes in disease. In Beresford, JN, and Owen, M (eds), Marrow stromal cell cultures, Cambridge University Press, 1998, pp 10-25 Bianco P, Gehron Robey P, Wientroub S: Fibrous dysplasia. In Glorieux F, ed, Pediatric Bone – Biology and Disease, Academic Press, New York 2003, pp 509-540 Bianco P & Gehron Robey P: Skeletal stem cells. In RP Lanza, HM Blau, DA Melton, MAS Moore, E. Donnall Thomas (Hon), C M Verfaillie, IL Weissman, MD West (eds): Handbook of adult and fetal stem cells, vol 2. Academic Press, New York 2004, pp 415-424 Ascenzi A, Bianco P, Fornaciari G, Rodriguez Martin C: Mummies from Italy, North Africa and the Canary Islands. In A Cockburn, E Cockburn, TA Reyman (eds) Mummies, disease, and ancient cultures. 2nd ed, Cambridge University Press, Cambridge, 1998, pp 1998, pp 263-282 Bianco P: Fibrous Dysplasia. In: WHO Classification of Tumours of Soft Tissue and Bone: Pathology and Genetics, 4th Edition; IARC 2012

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