Curriculum Vitae

Ashish Srivastava, PhD Plant Molecular Virology Laboratory, Center for Plant Molecular Biology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226 001 (U.P.), India Mobile: +91-9696696287, +91-8953207957 E-mail: [email protected]

Career objective To solve exciting problems and pursue cutting edge research in plant molecular virology and host parasitic interaction

Area of specialization:

Molecular

Biology,

Plant

Pathology,

Metabolomics

and

Biotechnology

Educational qualifications: 1.

Doctor of Philosophy (Ph. D.) in Biotechnology (submitted on 12-11-2014 in Rashtrasant Tukadoji Maharaj University, Nagpur, Maharashtra, India). Thesis title: “Molecular characterization and identification of begomovirus/es infecting Amaranth and Poppy grown in India.” Supervisors: Dr. S. K. Raj, Chief Scientist, CSIR- National Botanical Research Institute, Lucknow and Dr. S.S. Pande, Director, Rajiv Gandhi Biotechnology Centre, Rastrasant Tukadoji Maharaj Nagpur University, Nagpur, (M. S.).

2. Master of Science (M. Sc.) in Biotechnology (2010). DBT supported teaching programmeRajiv Gandhi Biotechnology Centre, Rastrasant Tukadoji Maharaj Nagpur University, Nagpur, (M. S.) 69.80%. 3. Bachelor degree (B. Sc.) in Bio-group (2008). Bundelkhand University, Jhansi (U.P.) 64%. 4. Intermediate (2004), Uttar Pradesh Board, Allahabad, Bio-group (Class XII) 61.40%. 5. High School (2002), Uttar Pradesh Board, Allahabad, Science-group (Class XI) 58.40%.

Experience: 4 years 1 months research experience in plant molecular virology. Currently I am working as a senior research fellow at Plant Molecular Virology Lab, CSIR-National Botanical Research Institute (NBRI), Lucknow, (U.P.), India. I have joined as a Junior Research Fellow in a DBT funded Project on 21th Oct, 2010 at Plant Molecular Virology Group, CSIR-National Botanical Research Institute (NBRI), Lucknow, (U.P.), India. I have promoted as senior research fellow on 21st Oct, 2012. I am working with Molecular characterization of DNA and RNA viruses and phytoplasma (member of wall-less bacteria family). I have basically expertise in characterization and identification of Begomovirus/es from various economically important crop plants and have to work for developing PCR, RT-PCR and NASH (Nucleic Acid Spot Hybridization) based protocols for the sensitive diagnosis of begomoviruses in economically important crop plants. I have established host parasite interaction and host response due to virus infection by histopathological and metabolomic means. Scientific Skills: I have expertise in following molecular biology and other techniques: Molecular Biology: Total genomic DNA/RNA isolation of viruses and phytoplasma from infected plants, Polymerase Chain Reaction (PCR), Nested-PCR, RT-PCR, Gel electrophoresis, Restriction Digestion, , Radio labeling, Southern hybridization, Molecular cloning in cloning and expression vectors, Plasmid DNA isolation, construction of Agroinfectious clones, SDS-PAGE, Western hybridization etc. Histopathological Analysis: Handling of light microscope, analysis and data interpretation of the images of transverse electron microscope (TEM) and scanning electron microscope (SEM). Metabolomics: Data analysis of GC-MS and HPLC. Plant Viruses: Whitefly and Mechanical inoculation of viruses on their main host plants and test species, Agro-infiltration, characterization and their maintenance in glass-house. Phytoplasmas: Phytoplamsa DNA isolation from plant samples, characterization by PCR mediated methods and RFLP analysis. Other: Good knowledge in nucleotide, amino acid sequence analysis using bioinformatics tools likes NCBI Data Base, EMBL Data Base, Primer designing, Sequence (nucleotide and amino

acid) analysis, BLAST, multiple sequence alignment, Mega 6 .0, Expasy, Clustal-W, Genomatix DiAlign, Recombination Detection Program, etc. and various other tools for genome analysis.

Professional achievements: 

Expertise to work with DNA and RNA viruses, including phytoplasma (member of wall less bacteria family).



Molecular characterization of Begomoviruses from various economically important crops.



Developed ELISA, PCR, RT-PCR, RFLP and NASH based protocols for the sensitive diagnosis of begomovirus in economically important plants.



Histopathological analysis by Electron microscope and Light microscope.



Study of metabolomic alterations caused due to virus infection.

Project/ Dissertation completed: 1. 3 months dissertation on “PCR amplification, cloning and sequence analysis of DNA-A genome of a begomovirus associated with mosaic disease of Jatropha species” at plant Molecular Virology Lab, National Botanical Research institute, Lucknow from 16-11-09 to 15-02-10.

Academic Achievments: 1. Qualify NET- June-2010 (Rank 0041/1300) conducted by CSIR-UGC against Roll No. 338091. 2. Qualify Combined Entrance Exam for Biotechnology (CEEB) (Rank 596) conducted by Jawaharlal Nehru University (JNU) against Roll No. 210209.

Personal information Father’s Name

:

Shri Purushottam Narayan Shrivastava

Mother’s Name

:

Smt. Kishori Devi Shrivastava

Date of Birth

:

12-Dec-1987

Gender

:

Male

Marital Status

:

Unmarried

Nationality

:

Indian

Languages known

:

Hindi, English

Address for correspondence :

Plant Molecular Virology Lab Centre for Plant Molecular Biology, CSIR- National Botanical Research Institute, Rana Pratap Marg, Lucknow-226 001, (U.P.)

INDIA

Hobbies

:

Playing Tabla, Listening Music and Reading.

Phone

:

+91-0522-297952

Mobile

:

+91-9696696287

Email ID

:

[email protected]

Permanent Address

:

H.N.151, R. P. Memorial Convent School, Dondaghat, Talabpura, Lalitpur (U.P.)

Referees: Dr. S. K. Raj Scientist-G & Senior Deputy Director, Head Plant Molecular Virology, Centre for Plant Molecular Biology Division, CSIR-National Botanical Research Institute Lucknow-226 001, (U.P.), India Mobile: +91-9415091366, E-mail: [email protected]

Dr. V. Hallan Plant Virology Laboratory, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur 176061, H. P., India Mobile: 9816812300 Email: [email protected]

Dr. B. P. Singh, FNAS Former Head & Emeritus Scientist, Plant Molecular Virology, CSIR-National Botanical Research Institute Lucknow-226 001, (U.P.), India Res. +91-9839125769 E-mail: [email protected]

Dr. S. U. Meshram Vice Chancellor, North Maharashtra University, Jalgaon (M.S.), and Former-Director, Rajiv Gandhi Biotechnology Centre, R.T.M. Nagpur University Nagpur (M.S.) Email: [email protected]

DECLARATION I hereby declare that the information furnished above is true to the best of my knowledge and I am solely responsible for its accuracy.

(AshishSrivastava)

LIST OF PUBLICATIONS Research publication in International Journals: 1. Ashish Srivastava, S. Kumar, M. Jaidi, S. K. Raj (2015). Characterization of an unidentified begomovirus associated with yellow mosaic disease of three ornamental Jatropha species grown in India. Virus Research. (Revision submitted).

IF: 2. 812

2. Ashish Srivastava, M. Jaidi, S. Kumar, S. K. Raj (2014). Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India. Archives of Virology. Doi: 10.1007/s00705-014-2288-8.

IF: 2.28

3. Ashish Srivastava, S. Kumar, S. K. Raj (2014). Molecular characterization of a begomovirus and betasatellite causing yellow vein net disease of Ageratum houstonianum. Plant disease. (doi: http://dx.doi.org/10.1094/PDIS-03-14-0333-RE).

IF: 2.74

4. Ashish Srivastava, S. Kumar, S. K. Raj, S. S. Pande (2014). Association of a distinct strain of Hollyhock yellow vein mosaic virus and Ludwigia leaf distortion betasatellite with yellow vein mosaic disease of hollyhock (Alcea rosea) in India. Archives of Virology. 159(10): 2711-5.

IF: 2.28

5. Ashish Srivastava, S. Kumar, S. K. Raj (2014). First report of Ageratum enation virus, betasatellite and alphasatellite causing leaf curl and enation disease of Amaranthus hypochondriacus in India. Plant Disease. Plant disease. 98(9): 1,285.1-1, 285.1.

IF: 2.74

6. Ashish Srivastava, S. K. Raj, S. Kumar, S. K. Snehi, A. Kulshreshtha, V. Hallan, S. S. Pande (2013). Molecular identification of Ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased Amaranthus cruentus in India. Virus genes. 47:584-590.

IF: 1.75

7. S. Srivastava, H. Bisht, O.P. Sidhu, Ashish Srivastava, P. C. Singh, R.M. Pandey, S. K. Raj, R. Roy, C.S. Nautiyal (2012). Changes in the metabolome and histopathology of Amaranthus hypochondriacus L. in response to Ageratum enation virus infection. Phytochemistry. 80:816.

IF: 3.35

8. M.S. Khan, A.K. Tiwari, S.K. Raj, Ashish Srivastava, S.H. Ji and S.C. Chun (2014). Molecular epidemiology of begomoviruses occurring on some vegetables, grain legume and weed species in the Terai belt of north India. Journal of Plant Diseases and Protection. 121 (2), 53–57.

IF: 0.605

9. Ashish Srivastava, S. Kumar, S. K. Raj (2014). Association of Pedilanthus leaf curl virus with yellow mottling and leaf curl symptoms in two jasmine species grown in India. Journal of General Plant Pathology. 80 (4): 370-373.

IF: 0.89

10. Ashish Srivastava, S.K. Raj, S. Kumar, S.K. Snehi (2013). New record of Papaya leaf curl virus and Ageratum leaf curl beta satellite associated with yellow vein disease of aster in India. New Disease Reports (2013) 28, 6. 11. Ashish Srivastava, S. K. Snehi, S. K. Raj (2013). First report of a variant of Pepper leaf curl Lahore virus on winter cherry in India. New disease Report.27:7. 12. S. K. Snehi, Ashish Srivastava, S. K. Raj, (2012). Biological characterization and complete genome sequence of a new begomovirus species associated with mosaic disease of Jatropha curcas in northern India. Journal of Phytopathology. 160:547–553.

IF: 1.0

ResearchGate Link: https://www.researchgate.net/profile/Ashish_Srivastava27 Membership:

Group member of Amarican Phytopathological Society. Registration Number: 220179

Oral/Poster/abstract presented in Conferences:

1. Ashish Srivastava, Meraj Jaidi, S. Kumar, S. K. Snehi and S. K. Raj (2013). Molecular identification of a begomovirus and betasatellite associated with yellow vein mosaic disease of hollyhock. national conference on "Microbes Promoting Crop Health, Productivity & Sustainability & Zonal Meeting, Indian Phytopathological Society (MEZ) (October, 26-27, 2013) at CSIR-Central Institute of Medicinal and Aromatic Plants ( CSIR-CIMAP), Lucknow and CSIR-National Botanical Research Institute (CSIR-NBRI), Lucknow. Oral Presentation. 2. Ashish Srivastava, Meraj Jaidi, S. Kumar, S. K. Snehi and S. K. Raj (2013). “Molecular identification of a begomovirus and betasatellite associated with yellow vein mosaic disease of hollyhock”. XXII International Conference on the topic "Asia Pacific Congress of Virology" from December 17-20, 2013 will be hosted by Amity Institute of Virology and

Immunology,Amity University Noida,India under aegis of Indian Virological Society. Poster presentation. 3. S. K. Snehi, Ashish Srivastava, S. K. Raj (2013). “Molecular studies and genetic diversity among the begomoviruses infecting Jatropha curcas in India”. 5th European Congress of Virology of European Congress of Virology (ECV), September 11-14, 2013.Lyon Convention Centre, France 11-14 September 2013. Abstract published. 4. Ashish Srivastava, S. K. Snehi, S. K. Raj and U. Pathre (2012). “Molecular characterization and genetic diversity among the begomoviruses infecting Jatropha curcas in India.” in the VIROCON-2012, XXI National conference on Immunobiology and management of viral diseases in 21st Century, IVRI Mukteshwar, Uttarakhand on November 8-10. Poster presentation. 5. Ashish Srivastava, S. K. Snehi and S. K. Raj (2011). “Biological and molecular characterization of a new begomovirus species associated with mosaic disease of Jatropha curcas in India” in the VIROCON-2011, XX National conference on Managing Emerging and Re-emerging Plnt, Animal, Human and Aquatic Viral Diseases, at National Research Centre on Equines, Hisar, Haryana, India on December 29-31, 2011. Poster presentation.