ARVO 2015 Annual Meeting Abstracts 504 High-resolution Blood Flow Imaging Thursday, May 07, 2015 8:30 AM–10:15 AM Exhibit Hall Poster Session Program #/Board # Range: 5296–5306/A0050–A0060 Organizing Section: Visual Psychophysics / Physiological Optics Contributing Section(s): Eye Movements/Strabismus/Amblyopia/ Neuro-Ophthalmology, Multidisciplinary Ophthalmic Imaging Program Number: 5296 Poster Board Number: A0050 Presentation Time: 8:30 AM–10:15 AM High resolution imaging of retinal vascular network geometry Jonathan Benesty1, 2, Edouard Koch2, David Rosenbaum3, Xavier Girerd3, José Sahel1, 2, Florence Rossant4, Isabelle Bloch5, Michel Paques1, 2. 1CHNO des Quinze Vingts, Paris, France; 2CIC 503 INSERM, Paris, France; 3Pitié-Sapetrière Hospital, Paris, France; 4 Institut Supérieur d’Electronique de Paris, Paris, France; 5Telecom ParisTech, Paris, France. Purpose: An energetically optimal vascular network should theoretically respect some physical principles among which the cubic mathematical relationship between parent and daughter vessels at bifurcations (Murray’s law). Deviations from this optimal geometry have been reported in several vascular diseases. The availability of high resolution imaging of vessels offers the opportunity to reevaluate these results. Here, following our initial evaluation of adaptive optics (AO) retinal vascular imaging (Koch et coll. 2014), we mathematically analyzed arterial and venous bifurcations to calculate more precisely the junction exponent and to evaluate the effect of cardiovascular risk factors. Methods: Flood-illumination AO imaging was done in 93 arterial and 25 venous bifurcations of 21 controls and 65 patients (mean age 45.7 years ±15.2; M/F 44/49) with various cardiovascular risk factors (hypercholesterolemia, hypertension, diabetes, smoking). A semiautomated procedure was used to measure vascular diameters and angles. Results: In control arteries, the mean value of the arterial junction exponent (theoretically equal to 3) was 2.80 (±0.44). The linear regression for measured vs predicted parent arterial diameter was 0.993 with a coefficient of determination R2=0.942. Mean arterial bifurcation angle measured was 85.05° (±11.7°), which was significantly different from the mean optimal angle 71.7°(±13.9°) (p0.05). Conclusions: Offset pinhole AOSLO provides the ability to noninvasively and quantitatively assess periarteriolar CFZ. This has the potential for better understanding of the retinal vascular development in the healthy retina and in disease states such as diabetic retinopathy and retinopathy of prematurity. Commercial Relationships: Toco Y. Chui, None; Nikhil Menon, None; Nadim Choudhury, None; Alexander Pinhas, None; Michael Dubow, None; Nishit Shah, None; Alfredo Dubra, Canon USA Inc (C), US patent 8,226,236 (P); Richard B. Rosen, Advanced Cellular Technologies (C), Advanced Cellulat Technologies (C), Allergan (C), Clarity (C), OD-OS (C), Opticology (I), Optovue (C) Support: Marrus Family Foundation, Wise Family Foundation, Glaucoma Research Foundation Catalyst for a Cure and RPB Career Development Award. Program Number: 5299 Poster Board Number: A0053 Presentation Time: 8:30 AM–10:15 AM Comparison of retinal vascular caliber measured by adaptive optics and from fundus photographs softwar. granados loic1, Edouard Koch2, Jonathan Benesty2, Florence Rossant3, Nicolas Lerme3, Max Villain1, Vincent Daien1, 4, Michel Paques2. 11Department of Ophthalmology, Gui De Chauliac Hospital, Montpellier, France; 2Clinical Investigation Center 503, Centre Hospitalier National des Quinze-Vingts, INSERM and Université Pierre et Marie Curie-Paris6, Paris, France; 33Institut Supérieur d’Electronique de Paris, Paris, France; 4Inserm, U1061, Montpellier, France. Purpose: Retinal vascular caliber analysis has been linked with increased cardiovascular risk and is predictive of cardiovascular pathology. The main purpose of this study was to compare 2 methods to evaluate retinal vascular caliber: IVAN® software from fundus photography and retinal vascular diameters from adaptive optics optics (AO) imaging. Secondary objective was to assess the relationships of each retinal vascular variables with systolic blood pressure (BP). Methods: 25 treatment-naive individuals underwent both AO imaging and fundus photography. Central Retinal artery equivalent (CRAE) was measured from fundus photographs using semiautomated standardized IVAN® software. Adaptive optics imaging of

the superotemporal retinal artery was performed and semi-automated segmentation allowed extracting parietal thickness and lumen diameter. Pearson’s correlation coefficient and Bland-Altman method were used to assess relationship between both technics of retinal vascular caliber assessment. Results: Mean (+/- standard deviation [SD]) CRAE from IVAN® software was 135.37 +/- 14.0 mm and mean arterial diameter from adaptive optics was 76.41+/- 11.7 mm. Mean systolic BP was 130.2 +/- 20 mmHg. Correlation coefficient between arterial diameter using AO and CRAE was 0.66 (p=0.0003). The biais between both technic (95% confidence interval) according to the Bland–Altman definition was 58.8 (54.7-63.2) mm. Inverse correlation was found between CRAE and wall to lumen ratio (r=-0.52, p=0.008). Systolic BP was significantly correlated with arterial parameters (r=-0.41, p=0.04; r=0.50, p=0.01 and r=0.71, p