19TH ANNUAL BUFFALO DNA REPLICATION AND REPAIR SYMPOSIUM FRIDAY June 19th, 2015 9:00 a.m.
CONTINENTAL BREAKFAST REGISTRATION & POSTER SETUP Grand Ballroom, Ramada Hotel & Conference Center WELCOME & OPENING REMARKS Mark Sutton, UB School of Medicine Session I – Chair: Anthony J. Berdis, Department of Chemistry, Cleveland State University COORDINATION OF DNA REPLICATION INITIATION AND TRANSCRIPTIONAL REGULATION BY HDA PROTEIN IN ESCHERICHIA COLI Babu, Vignesh M.P., Jamie C. Baxter and Mark D. Sutton Department of Biochemistry, University at Buffalo
THE ROLE OF MSH AND MLH MISMATCH REPAIR PROTEINS IN CAG/CTG REPEAT TRACT EXPANSION Williams, Gregory M., and Jennifer A. Surtees Department of Biochemistry, State University of New York at Buffalo, NY, USA
CHARACTERIZATION OF ESCHERICHIA COLI DNA POLYMERASE IV MUTATIONS IMPAIRED FOR FUNCTIONAL INTERACTIONS WITH THE DNA POLYMERASE III REPLICASE Scotland, Michelle K. 1, Justin M. H. Heltzel1, James Kath2, Jung-Suk Choi3, Anthony Berdis3, Joseph Loparo2, and Mark D. Sutton1 1 University at Buffalo, Department of Biochemistry, SUNY, Buffalo, NY 2 Harvard University, Department of Biological Chemistry & Molecular Pharmacology, Boston, MA 3 Cleveland State University, Center for Gene Regulation in Health and Disease, Cleveland, OH
CATALYSIS BY THE STAPHYLOCOCCUS AUREUS REPLICATIVE DNA POLYMERASE POLC IS REGULATED BY THE NEXT CORRECT INCOMING NUCLEOTIDE Pata, Janice1,2; Lahiri, Indrajit 1,2; Mukherjee, Purba 1,2; Johnson, Kenneth A. 3 1 Wadsworth Center, New York State Department of Health 2 Department of Biomedical Sciences, University at Albany, State University of New York, Albany, NY 12208 3 Institute of Cellular & Molecular Biology, University of Texas at Austin EVALUATING THE PRO-MUTAGENIC REPLICATION OF 8-OXOGUANINE Dasari, Anvesh1, Berdis A.J 1, 2, 3 * 1 Department of Chemistry and 2Center for Gene Regulation in Health and Disease, Cleveland State University, 2351 Euclid Avenue, Cleveland, OH 44115 3 Red5 Pharmaceuticals, LLC, 10000 Euclid Avenue, Cleveland, OH 44106
CHARACTERIZING AND CONTROLLING INTRINSIC BIASES OF LAMBDA EXONUCLEASE IN NASCENT STRAND SEQUENCING REVEALS PHASING BETWEEN NUCLEOSOMES AND G-QUADRUPLEX MOTIFS AROUND A SUBSET OF HUMAN REPLICATION ORIGINS Foulk, Michael S.13, Urban, John M.3, Casella, Cinzia23, Gerbi, Susan A.3 1 Mercyhurst University, Department of Biology, 501 East 38th Street, Erie, PA 16546 2 Institute for Molecular Medicine, University of Southern Denmark, JB Winsloews Vej 25, 5000 Odense C, Denmark,3Brown University Division of Biology and Medicine, 185 Meeting Street, Providence, RI 02912
FANCJ IS ESSENTIAL FOR GENOME-WIDE MICROSATELLITE STABILITY DURING REPLICATION STRESS Barthelemy, J.1, Hanenberg, H.2, and Leffak, M.1 1 Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, 45435 2 Departments of Pediatrics and Medical & Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202
LUNCH Grand Ballroom, Ramada Hotel & Conference Center INTRODUCTION: Jennifer Surtees, UB School of Medicine KEYNOTE ADDRESS
Stephen D. Bell, Ph.D. Professor and Chair Molecular and Cellular Biochemistry Department Indiana University Bloomington, IN
“DNA replication in the third domain of life” Session II – Chair: Elaine Sia, Department of Biology, University of Rochester 2:45 p.m.
ROLE OF THE C-TERMINAL DOMAIN OF THE HPV E1 DNA HELICASE IN DNA REPLICATION Melendy, Thomas 4, Monika Bergvall1,3, David Gagnon1,2, Steve Titolo1, Michaël Lehoux1,2, Caleb Homiski4, Claudia M. D’Abramo1,2, and Jacques Archambault1,2,3 1 Institut de Recherches Cliniques de Montréal (IRCM), Montreal, Quebec, Canada, 2 Department of Biochemistry and Molecular Medicine, Université de Montréal, Montreal, Quebec, Canada 3 Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada 4 Department of Microbiology & Immunology, University of Buffalo School of Medicine & Biomedical Sciences, Buffalo, NY
NUCLEAR ACCUMULATION OF THE PAPILLOMAVIRUS E1 HELICASE IS TIED TO MITOGENIC SIGNALING AND CELL CYCLE COMMITMENT Archambault, Jacques 1,2,3,4 Maxime Pindard 1,2, Catherine Spickler1, Neera Sriskandarajah1,3, David Gagnon1,4, and Claudia D’Abramo1. 1 Institut de Recherches Cliniques de Montréal (IRCM), 2 Dep. of Microbiology and Immunology, McGill University, 3 Division of Experimental Medicine, McGill University, 4 Dep. of Biochemistry and Molecular Medicine, Université de Montréal, Canada.
INHIBITION OF SV40 LARGE T ANTIGEN REPICATION FUNCTION BY DNA DAMAGE KINASE PHOSPHORYLATION Homiski, Caleb1; Fisk, John1, C.; King, Lauren2; Morgan, Iain2; Archambault, Jacques3; and Melendy, Thomas1,2 1 Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA 2 Henry Wellcome Institute of Comparative Medicine, University of Glasgow, Glasgow, UK 3 Molecular Virology Laboratory, Institut de recherches clinicques de Montreal (IRCM), Montreal, QC, CA
CHARACTERIZATION OF NOVEL FHA DOMAIN LIGAND BINDING MOTIFS Robertson, Aaron, Damir Mingaliev, Boris Simovic , Darryl R. Jones, Brendan J. McConkey and Bernard P. Duncker University of Waterloo, Ontario, Canada
THE RSC COMPLEX FUNCTIONS TO MAINTAIN PLOIDY IN SACCHAROMYCES CEREVISIAE Sing, Tina L. 1,2, Minnie Hung1,2, Shinsuke Ohnuki3, Charlie Boone2,4, Yoshikazu Ohya3 and Grant W. Brown1,2 1) Department of Biochemistry, University of Toronto, Canada; 2) Donnelly Centre for Cellular and Biomolecular Research; 3) Department of Integrated Biosciences, University of Tokyo; 4) Department of Molecular Genetics, University of Toronto, Canada
PHARMACOLOGICAL AND PRE-CLINICAL TESTING OF 5-NIDR AS A NEW THERAPEUTIC AGENT AGAINST BRAIN CANCER Kim, Seol 1, Jung-Suk Choi, Ph.D.2, and Anthony J. Berdis, Ph.D. 2,3 Department of Biology1, Department of Chemistry2, and the Center for Gene Regulation in Health and Disease3 Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115
IDENTIFICATION AND CHARACTERIZATION OF N6-METHYLADENOSINE DNA MODIFICATIONS IN THE BACILLUS SUBTILIS GENOME Nye, Taylor M., Schroeder, Jeremy W., Simmons, Lyle A. Department of Molecular, Cellular, and Developmental Biology University of Michigan, Ann Arbor
POSTER SESSION 4:30-6:30
SYMPOSIUM DINNER Outdoor Tent, Ramada Hotel & Conference Center
SATURDAY, June 20th, 2015 8:00 a.m.
CONTINENTAL BREAKFAST Grand Ballroom, Ramada Hotel & Conference Center Session III – Chair: Lyle Simmons, Department of Molecular, Cellular and Developmental Biology , University of Michigan THE SLIDING CLAMP TETHERS THE MUTL ENDONUCLEASE DOMAIN TO DNA Pillon, M.C.1, Babu, V.M.P.2,3, Randall, J.R.5, Cai, J.1, Sutton, M.D.2,3,4, Simmons, L.A.5 and Guarné, A.1 1 Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada. 2Department of Biochemistry and 3Witebsky Center for Microbial Pathogenesis and Immunology, and 4Genetics, Genomics and Bioinformatics Program. The School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, 14214. 5Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109.
A GENETIC INCOMPATIBILITY ACCELERATES ADAPTATION IN YEAST Bui, Duyen, T.*, Elliot Dine*, James B. Anderson+, Charles F. Aquadro* and Eric E. Alani* *Department of Molecular Biology and Genetics, Cornell University, and +Department of Biology, University of Toronto
SINGLE-MOLECULE MOTIONS AND INTERACTIONS IN LIVE CELLS REVEAL TARGET-SEARCH DYNAMICS IN MISMATCH REPAIR Simmons, Lyle A. 2, Yi Liao1†, Jeremy W. Schroeder2†, Burke Gao1, and Julie S. Biteen1 1Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA. 2Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
AN IMBALANCE IN MSH MISMATCH REPAIR FACTORS REVEALS A KEY REGULATORY STEP IN PREVENTING RECOMBINATION BETWEEN DIVERGENT DNA SEQUENCES Chakraborty, Ujani, Carolyn George, Amy Lyndaker, and Eric Alani Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY
CTP1 TETRAMERS ORCHESTRATE DNA BINDING AND BRIDGING IN DNA DOUBLE STRAND BREAK REPAIR Andres, S.N., Appel, C.D., Westmoreland, J., Williams, J.S., Nguyen, Y., Robertson, P.D., Resnick, M.A., and Williams, R.S. Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences
FUNCTIONS OF FUN30 CHROMATIN REMODELER IN REGULATING CELLULAR RESISTANCE TO GENOTOXIC STRESS Bi, Xin, Yu, Qun, Siler, Jasmine, Li, Chong and Khan, Ali Department of Biology, University of Rochester, Rochester, NY 14627, USA
COFFEE BREAK Grand Ballroom, Ramada Hotel & Conference Center
Session IV – Chair: Tom Melendy, Department of Microbiology, University at Buffalo 10:15 a.m.
DNA LIGASE IV AND MYOGENIC DIFFERENTIATION Cuesta-Sancho, Sara 1, Ouchi Mutsuko 1, Ouchi Toru 1. 1 Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets Buffalo, NY 14263
SLX5/SLX8 REGULATION OF THE SUMO/UBIQUITIN NETWORK ALLEVIATES REPLICATION STRESS IN MCM10 DEFICIENT CELLS. Thu, Yee Mon1, Van Riper, Susan2, Higgins, LeeAnn2, Zhang, Tianji1, Markowski, Todd2, Nguyen, Hai Dang3, Griffin, Timothy2, and Anja-Katrin Bielinsky1 1 University of Minnesota, Department of Biochemistry, Molecular Biology and Biophysics, 2 Center for Mass Spectrometry and Proteomics, University of Minnesota, Saint Paul, MN, 55108, 3 Massachusetts General Hospital Cancer Center, Harvard Medical School.
INVESTIGTATING HOW THE DNA DAMAGE RESPONSE AND STEM CELL PROPERTIES OF TESTICULAR GERM CELL CANCERS INFLUENCE CHEMOSENSITIVITY Pierpont, Timothy M.1, Lyndaker, Amy M.1, Anderson, Claire M.1, Roden, Jamie L.1, Bagepalli , Lina1, Kataria, Nandita1, Braxon, Alicia1 , Hu, Hilary1, Garness, Jason2, Cook, Matthew S.2, Capel, Blanche2, Schlafer, Donald H.1, Southard, Teresa1, and Weiss, Robert S1. 1 Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA and 2Department of Cell Biology, Duke University Medical Center, Durham, NC, USA
RAD5 FUNCTION IN THE S. CEREVISIAE REPLICATION STRESS RESPONSE Gallo, David, Ashruth Narula and Grant W. Brown. Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada
DAMPENING DNA DAMAGE CHECKPOINT SIGNALLING VIA COORDINATED BRCTDOMAIN INTERACTIONS Cussiol, José R1, Carolyn M Jablonowski1, Askar Yimit2, Grant W Brown2 and Marcus B Smolka1 1 Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, U.S.A. 2 Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, M5S 3E1, Canada
5’ FLAP-DEPENDENT ACTIVATION OF POSTREPLICATIVE REPAIR Becker, Jordan R.1, Carles Pons2, Hai Dang Nguyen1#, Michael Costanzo3, Charles Boone3, Chad L. Myers2, and Anja-Katrin Bielinsky1 1 Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America, 2Department of Computer Science and Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America, 3 Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada, #Current Address: Massachusetts General Hospital Cancer Center; Harvard Medical School; Charlestown, Massachusetts,
ASSEMBLY OF SLX4 SIGNALING COMPLEXES BEHIND DNA REPLICATION FORKS Balint, Attilaa, TaeHyung Kima, David Galloa, Jose Renato Cussiolb, Francisco M Bastos de Oliveirab, Jiongwen Oua, Ryuichiro Nakatoc, Alexey Gurevicha, Katsuhiko Shirahigec, Marcus B Smolkab, Zhaolei Zhanga and Grant W Browna a University of Toronto, Toronto, Ontario, Canada. b Cornell University, Ithaca, New York, c University of Tokyo, Tokyo, Japan.
CLOSING REMARKS Tom Melendy, UB School of Medicine