YOUR DNA POLYMERASES

A g u a r a n t e e d q u a l i t y i n a n y a p p l i c at i o n

| D N A P o ly m e r a s e s

DNA polymerases brochure p.2] p.3]

PCR Enzyme Selection Guide Introduction

p.4] p.7] p.8] p.9]

The Diamond Taq® family Diamond & Red Diamond Taq® Hot Diamond Taq® HGS Diamond Taq®

p.10] Other Life Science enzymes p.11] DAp GoldStar® DNA polymerase p.12] SilverStar DNA polymerase p.13] AccuStartm DNA polymerase p.14]

Ready-to-use PCR mixes GoldStar®/HGS

p.15]

Related products



PCR Enzyme Selection Guide Free Sample

Page

PCR qPCR Yield Sensitivity Fidelity Specificity Visual Difficult Long recognition template template Low copy (GC & AT rich) template

3’ end

Regular Polymerase 7

Diamond Taq®

7

Red Diamond Taq

TAQ-I041-100

11

DAp GoldStar ®

ME-0068-SA

12

SilverStar

ME-0074-SA

***

13

Accustar

ME-0067-SA

14

GoldStar Mix

PK-0064-02SA

14

Red'y Gold Mix

PK-0064-02RSA

TAQ-I021-100 ®

TM

***

*

++

++

++

++

***

*

++

***

*

++

*

+++

***

*

***

*

***

*

++

++

++

*

**

3’ A

++

+++

++

***

***

3’A + Blunt

+++

+

+

*

**

3’ A

++

+

++++

++

*

*

Blunt

++

++

++

++

*

**

3’ A

*

++

++

++

++

*

**

3’ A





**

3’ A

Hotstart Polymerase 8

Hot Diamond Taq®

TAQ-I033-100

***

**

+++

+++

++

+++

***

***

3’ A

9

HGS Diamond Taq

TAQ-I011-100

***

***

+++

+++

++

+++

**

**

3’ A

14

Hot GoldStar Mix

PK-0073-02SA

***

***

+++

+++

++

+++

14

Red'y Star Mix

PK-0073-02RSA

***

***

+++

+++

++

+++

®

* Weakly recommended

** Recommended

*** Highly recommended

+ Low

++ Medium

+++ High

++++ Very high



**

**

3’ A

**

**

3’ A

93

Many applications require DNA polymerases with the highest quality standards in purity and

Introduction

production processes. Diamond Taq® family enzymes are developed and produced according to cGMP compliant

Polymerase Chain Reaction (pcr) is nowadays established as a core technology in R&D and for diagnostic purpose. In both fields, you need a product you can rely on durably as well as flexible services ensuring optimal nucleic acid amplification.

processes, ensuring the highest reproducibility, consistency and sensitivity in every PCR applications.

High performance DNA polymerases are purified according to multiple purification protocols minimizing the amount of E. coli DNA trace present in the final Taq polymerase preparation, allowing you to work with minimal background. All our enzymes are tested extensively for the absence of nicking and priming activities, exonucleases and non-specific endonucleases. l

These enzymes have been easily validated by top laboratories and several major diagnostic companies. n

Classified Cleanrooms (ISO 7/8). IVD Validated Methods, Qualified Equipments. Batch Records, Traceability, ISO 13485.

Partnership for all needs

First Idea

Life Science Taq polymerases

Feasability Design Control Validation

Diamond Taq® polymerases

i n f o @ e u r o g e n t e c . c o m w w w. e u r o g e n t e c . c o m

Commercialization

| D N A P o ly m e r a s e s

Additional information Documentation] Enzymes are provided with specification sheet & Certificate of Analysis with the QC data released by a QC authorized

The Diamond Taq® family

person and based on review of the complete batch record. Order & Shipping conditions] Diamond Taq® family enzymes can be ordered & shipped following two

All the Diamond Taq® enzymes have been manufactured under fully compliant cGMP conditions, purifications and quality controls to offer you the maximum security in your assay.

processes: IVD label]

k Shipping on dry ice. k Full traceability from

Diamond Taq® family enzymes are highly thermostable polymerases produced and purified from recombinant Escherichia coli bacteria containing the Thermus aquaticus DNA Polymerase gene.

production, storage to shipment of the product.

k Tracking number sent to

The expressed enzyme shows very good fidelity and catalyzes 5’>3’ synthesis of DNA with no detectable 3’>5’ exonuclease activity. The enzyme has the “extendase” activity allowing TA cloning. l

customer the day of the shipment. Research label]

k  Shipping at room temperature

EOS

(except for Hot DiamondTaq®, Eurogentec Ordering System on dry ice).

k k

k  Full traceability from

Higher Sensitivity Higher lot-to-lot consistency ¡ Strickly monitored GMP and analytical processes ensuring lot-to-lot consistency. k Ultra-low bioburden ¡ Production in a GMP-Pharma facility leading to a guaranteed bioburden between 0 and 10 CFU/ml. k Ultra-low residual DNA content ¡ QC-tested ensuring less than 1 fg (0.2 copy) of genomic E. coli DNA / Taq unit. k Lower risk of false positive results due to residual DNA contamination (bacterial & fungal).

production to storage of the product.

✔

EOS Eurogentec Ordering System

GoldStar® ] b ecomes RedGoldStar ] becomes

Diamond Taq® qualities

Diamond Taq® Red Diamond

HotGoldStar ] b ecomes HGS Diamond Taq®

From now on GoldStar® enzymes are also perfectly suited for diagnostic and stringent PCR & qPCR applications. Manufacturing processes are fully compliant to the ISO13485:2003 Medical Device standard.

95

Diamond Taq® family enzymes processes Diamond Taq®

Chemical modification

Inert Red Dye

Hot modification Eurogentec patent

HGS Diamond Taq®

Red Diamond Taq®

Hot Diamond Taq®

A guarantee of quality in any application

Core enzyme specifications Parameters

Specifications

Appearance2

Clear, colorless solution1

Identity (SDS-PAGE)

MW approx. 95 kDa

Volume activity

5 U/µl

Purity (SDS-PAGE)

Over 98  %

Performance test: PCR -lDNA 2 Performance test: PCR - 18 S DNA

0.5 kb fragment positive down to 5 pg 0.1 kb fragment positive down to 10 pg

2

Ribonucleases (up to 10 U, 1 h, 37  °C)

Not detectable

Endonucleases (up to 30 U, 16 h, 65  °C)

Not detectable

Additional specifications:

Exonucleases (up to 30 U, 16 h, 65  °C)

Not detectable

Parameters

Nicking activity (up to 30 U, 16 h, 65  °C)

Not detectable

For HGS Diamond Taq HotStart activity

Specifications ®

E. coli residual DNA

Over 1 fg / Taq Unit

Bioburden2

≤ 10 CFU/ml

Stability2

36 months (at -20  °C) from date of manufacture

For Hot Diamond Taq®

None

Performance test PCR-Numb DNA

Animal-derived additives2 Red Solution for Red Diamond Taq

1 2

®

Also for buffer & MgCl2

i n f o @ e u r o g e n t e c . c o m w w w. e u r o g e n t e c . c o m

No detectable amplification without 95 °C activation

0.3 kb fragment positive down to 10 pg

| D N A P o ly m e r a s e s

Diamond Taq® polymerases manufacturing processes

Diamond Taq® polymerases in (pre)commercial assays

are performed under

Choosing a manufacturing partner early in the assay development process is an important step towards successful assay validation and product commercialization. Every aspect of the manufacturing process is monitored and documented, providing our partners with a high level of confidence in quality and regulatory compliance. l

controlled conditions; in class 10 000 cleanroom for purification (ISO 7 with < 100 cfu/m³) and in class 100 laminar flow hoods for fill & finish (ISO 5 in with < 1 cfu/m³). Final product is released

Professional

Over 25 years experience in Oligonucleotide synthesis

by our Quality Assurance

Over 25 years experience in GMP protein manufacturing

Department with a formal

CMO for bulk reagents & GMP Oligonucleotides

Certificate of Analysis.

Skilled & fully trained staff

Full batch records are

Expert chemists in synthesis & purification

maintained and can be reviewed during customer

Quality Commitment

Organization-wide QMS implementation Comprehensive risk analysis and mitigation

or regulatory audits. n

Classified cleanroom facility Full compliance to – ISO 13485:2003 – FDA cGMP/QSR (21 CFR Part 820) – EU IVD Directive 98/79 EC compliance

Flexibility

Custom Fill & Finish, labeling & packaging available Assay dispensing service Dedicated Project Manager and Technical Specialist Proven successful and timely delivery of assay component

Eurogentec Headquarters

+32 4 372 74 00

+32 4 372 75 00

[email protected]

w w w. e u r o g e n t e c . c o m

97

Additional information

The red dye does not influence the PCR reaction and has no effect on automated or

Diamond & Red Diamond Taq®

manual sequencing, restriction digestions or other downstream applications. Removing the dye can be easily accomplished using any standard purification

Originally supplied for Research applications, GoldStar ® & RedGoldstar Taq DNA Polymerases, are now manufactured according to a GMP Process and have been renamed Diamond Taq® and Red Diamond Taq®, improving their suitability towards in vitro Diagnostic & demanding Research applications. l

Benefits

method.

Package content

k k

Same enzyme as the extensively validated GoldStar ®. C  ustomized Fill & Finish, on request Diamond Taq® enzyme can be produced with: ¡ An activity from 5 to 200 U/µl ¡ A glycerol level from 1 to 50 % k V isual confirmation of pipetting (inert red dye) included in the storage buffer of Red Diamond Taq®.

k Diamond Taq® or Red Diamond Taq® (5 U/µl)

k

10x reaction buffer

k 25 mM MgCl2 solution

Diamond & Red Diamond Taq® Process

Diamond Taq® Red Diamond Taq®

IVD process

TAQ-I020-XXX

TAQ-I040-XXX

Classical process

TAQ-I021-XXX

TAQ-I041-XXX

xxx refers to the unit amount (1000-5000-25000)

Performance tests

Smart Ladder

Diamond Taq®

Competitor GMP Taq

Diamond Taq® performance is similar or higher than competitor GMP Taq® Diamond Taq® was evaluated against competitor GMP Taq® for its ability to amplify different amounts (from 5 to 1000 pg) of a 0.5 kb PCR template (λ DNA). PCR products were analyzed by gel electrophoresis.

DNA (pg): 1000 100 50 5 1000 100 50 5

Diamond Taq®

Eurogentec GoldStar Taq®

DNA (pg): 1000 100 50 5 1000 100 50 5

Diamond Taq® and GoldStar® Taq have the same performance Diamond Taq® was evaluated against Eurogentec’s GoldStar Taq® for its ability to amplify different amounts (from 5 to 1000 pg) of a 0.5 kb PCR template (λ DNA). PCR products were analyzed by gel electrophoresis.

| D N A P o ly m e r a s e s

Hot Diamond Taq® HotStart characteristics preventing non-specific polymerisation are accomplished through a proprietary agent, not via a chemical modification nor a blocking antibody; this prevents primer-dimer formation and increases the PCR yield of specific products. Hot Diamond Taq® shows no amplification at room temperature and gives very high yield of specific product. The enzyme needs very short activation time (100  % activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 min. at 95  °C). l

Benefits k Universal, amplification of long & difficult templates. k Specific, prevents non specific polymerisation as primer-dimer formation and increases the PCR yield of specific products. k Very short activation time (minimum 20 sec.) Package content

Hot Diamond Taq®

k Hot Diamond Taq® (5 U/µl)

Process

k 10x reaction buffer k 25  mM MgCl2 solution

Hot Diamond Taq®

IVD process

TAQ-I032-XXX

Classical process

TAQ-I033-XXX

xxx refers to the unit amount (1000-5000-25000).

Ideal for difficult and GC rich templates amplifications

HDT C1C1 C2 C2 C3 C3 C4 C4 HDT

HDT C1

C2

C3

C4

DT DT

Performance test on a difficult template: Hot Diamond Taq ® (HDT) & Diamond Taq ® (DT) were evaluated against HotStart competitors (C1, 2 & 4 - Chemical modification ; C3 - Antibody) for their ability to amplify a difficult PCR template (gDNA NUMB - 306b). PCR products were analyzed by gel electrophoresis.

DT

Performance test on a 72 % GC rich template: Hot Diamond Taq ® (HDT) & Diamond Taq ® (DT) were evaluated against HotStart competitors (C1, 2 & 4 - Chemical modification ; C3 - Antibody) for their ability to amplify a GC rich PCR template (SCO3449 - 152b). PCR products were analyzed by gel electrophoresis.

99

HGS Diamond Taq® Originally supplied for Research applications only, HotGoldStar Taq DNA Polymerase is now manufactured according to a GMP Process and has been renamed HGS Diamond Taq®, improving its suitability towards in vitro Diagnostic & demanding Research applications. HGS Diamond Taq® is a chemically modified HotStart DNA polymerase, which initially lacks all activity below 74  °C. It requires a thermal activation of 10 minutes at 95 °C to reach maximal activity. This avoids non-specific priming at low temperature during the PCR set-up. HGS Diamond Taq® is more heat-stable than commonly used Taq® DNA polymerase. DNA fragments as long as 2 kb can be efficiently amplified. HGS Diamond Taq® DNA polymerase provides efficient amplification of specific products without amplifying non-specific products or primer dimers. l

HGS Diamond Taq® HGS Diamond Taq®

Process IVD process

TAQ-I010-XXX

Classical process

TAQ-I011-XXX

Benefits k Prevents

non specific polymerisation primer-dimer formation and increases the PCR yield of specific products. k Proven performance in many applications, same enzyme as extensively validated and benchmarked HotGoldStar ®. k Customized Fill & Finish, on request HGS Diamond Taq® enzyme can be produced with: ¡ An activity from 5 to 50 U/µl ¡ A glycerol level from 1 to 50 %

Package content

k HGS Diamond Taq® (5 U/µl) k 10x reaction buffer

xxx refers to the unit amount (1000-5000-25000).

k 25 mM MgCl2 solution

Performance tests HGS Diamond Taq® is a chemically modified HotStart enzyme. HGS Diamond Taq ® is a HotStart chemically modified enzyme that shows no amplification at room temperature but gives very high yield of specific product after a minimum of 10 minutes of enzyme activation at 95 °C.

HGS Diamond Taq®

Eurogentec HotGoldStar

HGS Diamond Taq® and HotGoldStar® Taq performances are similar. HGS Diamond Taq ® was evaluated against Eurogentec’s HotGoldStar (HGS) for its ability to amplify in triplicate 5 pg of a 0.5 kb PCR template (λ DNA). PCR products were analyzed by gel electrophoresis.

| D N A P o ly m e r a s e s

Other Life Science enzymes Eurogentec offers other premium quality PCR enzymes with different characteristics. For optimal results, it is critical to choose the enzymes that suit your application. Please refer to the PCR selection table (p.2).

DAp GoldStar® DNA polymerase

Specially designed for applications requiring high processivity and high yields. Suggested for difficult and impure fragments.

SilverStar DNA polymerase

Extremely robust Taq DNA polymerase giving high yield. Ideal for DNA array.

AccuStartm DNA polymerase

High Fidelity DNA Polymerase producing blunt-ended amplicons. Recommended for impure template amplification.

9 11

Additional information Storage conditions] Storage at -20 °C is recommended.

DAp GoldStar® DNA polymerase

Shipping conditions] Shipped at -20 °C. Quality Control] The DAp GoldStar® is tested extensively for activity, non-specific endonuclease /

DAp (Difficult Amplification) GoldStar® is a highperformance complex of enzymes and additives, specially designed for applications requiring high processivity and high yields. DAp GoldStar ® possesses 5’Ä3’ DNA polymerase activity and 3Ä5’ exonuclease activity which prevents misincorporations during primer extension. In contrast with other proofreading enzymes, DAp GoldStar ® does not degrade primers. DAp GoldStar ® contains an additive, which eliminates the sequencedependent removal of 3’-terminal dNMPs in growing DNA chains (pyrophosphoro-lysis). l

enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72 °C.

Package content

polymerase (4 U/µl)

High processivity (3’ to 5’ exonuclease activity). High yields. Long range applications up to 30 kb genomic DNA. Higher fidelity than a regular Taq polymerase. Suitable for difficult templates. Suitable for TA cloning.

DAp GoldStar® DNA polymerase Description

Unit definition] The amount of

k DAp GoldStar® DNA

Benefits k k k k k k

nickase and exonuclease activity.

Quantity

Reference

500 Units

ME-0068-05

DAp GoldStar DNA polymerase ®

i n f o @ e u r o g e n t e c . c o m w w w. e u r o g e n t e c . c o m

k 10x Optibuffer without MgCl2 k 50 mM MgCl2 k DMSO Note: For long template amplification, the addition of glycerol or DMSO to a final concentration of 2 - 8 % may improve performance.

| D N A P o ly m e r a s e s

Additional information

Storage conditions] Storage at - 20 °C is recommended. Shipping conditions] Shipped at - 20 °C. Quality Control] It is specially purified to remove traces of DNA

SilverStar DNA polymerase

that could interfere with some reactions and is free from all nonspecific endonucleases, nickases and exonucleases. Unit definition] One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72 °C.

SilverStar is a high-performance thermostable recombinant DNA polymerase, isolated from Thermus aquaticus. It is an extremely robust Taq DNA polymerase that does not require optimisation and gives high yields with minimal background consistently from one reaction to another. It is a highly active enzyme that produces excellent results across a wide range of applications such as DNA Array. The SilverStar has a 5’Ä3’ exonuclease activity and also the extendase activity allowing TA cloning. The enzyme lacks the 3’Ä5’ exonuclease activity. l

Benefits Package content

k SilverStar DNA polymerase (5 U/µl)

k

10x reaction buffer

k

k High yields. k Robust PCR (up to 10 kb). k Highly purified Taq polymerase. k No optimisation needed.

50 mM MgCl2

Robust amplification SilverStar DNA polymerase Description

Quantity

Reference

SilverStar DNA polymerase

500 Units

ME-0074-05

SilverStar dilution buffer

1 ml

ME-0000-DI

SilverStar reaction buffer

1.5 ml

ME-0000-RE

In DNA Array application, 50,000 ORFs from more than 10 different organisms have been successfully amplified. i n f o @ e u r o g e n t e c . c o m w w w. e u r o g e n t e c . c o m

9 13

Additional information

Storage conditions] Storage at -20 °C is recommended. Shipping conditions]

AccuStar™ DNA polymerase

Shipped at -20 °C. Quality Control] It is specially purified so as to remove traces of DNA that could interfere with some

AccuStarTM DNA polymerase is a thermostable enzyme possessing 5’Ä3’ DNA polymerase and 3’Ä5’ exonuclease activities, offering extremely high-fidelity (up to 47-fold higher fidelity than Taq). Regular Taq AccuStarTM DNA polymerase produces blunt-ended amplicons of up to 5  K b in length.

reactions and is free from all non-

AccuStarTM DNA polymerase is supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (2 mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, an additional MgCl 2 tube is provided. The specificity and performance of AccuStarTM DNA polymerase can be further improved with the use of 2x Booster Mix (not supplied), which is designed for GC or ATrich DNA and templates with difficult melting profiles. l

incorporates 10 nmoles of dNTPs

specific endonucleases, nickases and exonucleases. Unit definition] One unit is defined as the amount of enzyme that into acid-insoluble form in 30 minutes at 72 °C.

Package content

k AccuStar™ DNA polymerase (2.5 U/µl)

Benefits

k k

k High-fidelity coupled with high yield. k Amplifies fragments up to 5 Kb. k Ultra-high fidelity for subsequent cloning. k Blunt-end cloning.

10x reaction buffer 50 mM MgCl2

High fidelity & high yield amplification MW

AccuStar™ DNA polymerase 1.5 1 0.8

Description

Quantity

Reference

AccuStar DNA polymerase

500 Units

ME-0067-05

Booster Mix

2 x 1.2 ml

ME-0076-02

TM

0.6

1

2

3

4

5

6

7

8

A serial dilution of template was performed to demonstrate the high performance of Accustar™, even at low DNA concentrations.

| dna p o l y m e r a s e s C u s t o m Ser vic es

Enabling Kits & Reagents

C u s t o m Ser vic es

Enabling Kits & Reagents

Red loading dye

Ready to use PCR mixes

No loading buffers or tracking dyes required.

GoldStar® Mix / Red’y’Gold Mix

Samples may be added directly to an agarose

2 x concentrated PCR mixes are convenient premixed solutions that include in a single tube all common reagents needed for PCR. Simply dilute twice the PCR mix with template, primers and water to start your reaction. The elimination of cumbersome preparation steps prevents contamination risk by multiple pipetting. Mixes usage also increases the reproducibility of PCR reactions. The Red’Y’Gold Mix includes a colored loading buffer allowing the direct loading of the amplicons on a gel. l

gel after PCR without the addition of a loading buffer or tracking dye. The red dye migrates as a 400500 bp fragment in agarose gel 1 % in TBE buffer. n

HotGoldStar Mix / Red’y’Star Mix For amplifications requiring high specificity use mixes containing the HotGoldStar Taq polymerase, which completely lacks any activity before the activation step, avoiding non-specific annealing at low temperature. The Red’y’Star Mix includes a colored loading buffer allowing the direct loading of the amplicons on a gel. l

Note: Mixes prepared under ISO 13485: 2003 compliant processes (including enzymes of the Diamond Taq® family) are available on request. Please, contact [email protected]

Benefits k Easy-to-use, 2x concentrated, premixed MasterMixes. k Optimized for a broad range of targets (< 3 Kb). k Highest sensitivity and yield. k Robust amplification of GC-rich sequences. k A chieves the highest quality sequencing data. k Optionally includes a red loading dye.

Ready-to-use PCR mixes Product

Reference

Quantity

RXNs

GoldStar Mix 2x

PK-0064-02

5 x 1 ml

200

Red’y’Gold Mix 2x

PK-0064-02R

5 x 1 ml

200

HotGoldStar Mix 2x

PK-0073-02

5 x 1 ml

200

Red’y’Star Mix 2x

PK-0073-02R

5 x 1 ml

200

Kit Content GoldStar ® Taq polymerase (1 U/25μl), dNTPs (400 μM), MgCl2 (3 mM), Buffer HotGoldStar Taq polymerase (1 U/25μl), dNTPs (400 μM), MgCl2 (3 mM), Buffer

Red loading dye  

9 15

EOS

Related products

Eurogentec Ordering System All the reagents you may need for nucleic acid amplification are available online on:

www.eurogentec.com via EOS (Eurogentec Ordering System)

License statments

k     Hot Diamond Taq Hot Diamond Taq

®

®

products

products are covered by

international EUROGENTEC pending Patent application (PCT/EP2008/067435). Hot Diamond

k Custom and Universal Primers and Probes k dNTPs k Extraction kits k Reverse transcriptase k Agarose and Mupid® electrophoresis apparatus k Molecular Weight Markers k Cloning and expression kits (Staby™ technology)

✔

EOS Eurogentec Ordering System

Taq® products are sold exclusively for Research use only by the purchaser and may not be used for clinical or diagnostic purposes, resold, distributed or re-packaged without the prior written consent of EUROGENTEC S.A. It may be necessary to obtain a separate license for certain patented applications in which the Hot Diamond

Taq® products are used.

k    Diamond & Hot Diamond Taq

®

Polymerases Use of this product is covered by the following US patent: 6,127,155. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

k    Chemically-Modified Hot-Start Polymerase Reagents and Kits Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 6,127,155 5,677,152 (claims 1 to 23 only), 5,773,258 (claims 1 and 6 only), and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of

Information

@ [email protected] @ [email protected]

International Toll free number: 00 800 66 60 01 23 

this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own intemal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other

Trademarks labels

k AccuStar is a registered trademark of Eurogentec S.A. k Diamond Taq is a registered trademark of Eurogentec S.A. k GoldStar is a registered trademark of Eurogentec S.A. k Mupid is a registered trademark of Advance Co., Ltd. k Staby is a trademark of Delphi Genetics S.A. tm

®

®

®

i n f o @ e u r o g e n t e c . c o m w w w. e u r o g e n t e c . c o m

commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. l

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