With you at every step
2014 Product Catalogue
farm animal immunodiagnostics
www.id-vet.com
WITH YOU AT EVERY INNOVATIVE FARM ANIMAL DIAGNOSTICS
Ordering information IDvet’s sales team and worldwide distribution network are at your service. Please contact IDvet for technical support, or to locate the representative nearest you.
Located in Montpellier, France, IDvet develops, manufactures and sells diagnostic reagents for the detection of infectious diseases in farm animals. With a focus on immunodiagnostics, and ELISA kits in particular, IDvet offers an extensive range of products for emerging diseases and diseases included in major prevention programs. IDvet’s tests are used by private and public laboratories in surveillance programs and prevalence studies in over 70 countries. HIGH-QUALITY PRODUCTS IDvet ELISA kits, marketed under the tradename ID Screen®, contain all necessary reagents for sample testing, and are standardized to detect national and international reference sera. The tests are robust and easy-to-use, and show innovation through simplified test protocols, ready-to-use reagents, and improved test performance. Thanks to a highly-skilled scientific team, kit components are produced on-site to guarantee reagent quality.
310, rue Louis Pasteur 34790 Grabels - FRANCE Phone + 33 (0) 4 67 41 49 33 Fax + 33 (0) 4 67 45 36 95
[email protected] www.id-vet.com
Certified ISO 9001 : 2008, the company’s commitment to quality covers all activities, from Research & Development, to Production, Quality Control and Sales.
STEP SCIENTIFIC KNOW-HOW IDvet strives to be at the cutting edge of veterinary diagnostics. IDvet is the industrial partner in a number of European research programs, and collaborates closely with many international and national reference laboratories. WITH YOU AT EVERY STEP IDvet’s multi-lingual sales and technical team aims to provide the highest-level of customer service, offering efficient, professional technical support. By adapting products to meet your needs, rapidly responding to your queries, providing free supplementary liquid reagents, and assisting you in kit use and result interpretation, IDvet is with you at every step.
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CoNTENTS RUMINANTS............................................................................... 7
Akabane................................................................................................................................8 Besnoitiosis...........................................................................................................................9 BHV-4..................................................................................................................................10 Bluetongue..........................................................................................................................11 Bovine Leukosis Virus.........................................................................................................14 Bovine Viral Diarrhoea (BVD) / Border Disease (BD) / Mucosal Disease (MD)..................17 Brucellosis...........................................................................................................................20 Chlamydophila abortus / Enzootic abortion.........................................................................24 Interferon gamma................................................................................................................25 Infectious Bovine Rhinotracheitis (IBR)...............................................................................26 Maedi Visna Virus / Caprine Arthritis Encephalitis Virus (CAEV)........................................30 Neospora caninum..............................................................................................................31 Parainfluenza 3...................................................................................................................32 Paratuberculosis / Johne’s Disease....................................................................................33 Peste des Petits Ruminants................................................................................................34 Q Fever...............................................................................................................................37 Rift Valley Fever..................................................................................................................38 Schmallenberg virus............................................................................................................40 Toxoplasmosis.....................................................................................................................43
EQUINE..................................................................................... 45
Equine Infectious Anemia....................................................................................................46 Equine Viral Arteritis............................................................................................................49 Influenza A...........................................................................................................................50 Strangles / Streptococcus equi. ..........................................................................................52 Trichinellosis........................................................................................................................53 West Nile Virus....................................................................................................................54
AVIAN........................................................................................ 57
Infectious Bronchitis............................................................................................................58 Infectious Bursal Disease / Gumboro Disease....................................................................60 Infectious Laryngotracheitis.................................................................................................62 Influenza A...........................................................................................................................63 Avian Mycoplasmosis..........................................................................................................66 Newcastle Disease..............................................................................................................70 Salmonellosis......................................................................................................................72 West Nile Virus....................................................................................................................73
SWINE....................................................................................... 75
Actinobacillus pleuropneumoniae. ......................................................................................76 Aujeszky’s Disease / Pseudorabies.....................................................................................78 African Swine Fever............................................................................................................80 Brucellosis...........................................................................................................................81 Classical Swine Fever / Hog Cholera..................................................................................82 Hepatitis E...........................................................................................................................83 Influenza A...........................................................................................................................84 Mycobacterium avium.........................................................................................................86 Mycoplasma hyopneumoniae / Enzootic pneumoniae........................................................87 Swine Vesicular Disease.....................................................................................................89 Toxoplasmosis.....................................................................................................................90 Trichinellosis........................................................................................................................91
COMPANION ANIMALS........................................................... 93
Canine Leishmaniasis.........................................................................................................94
ALL SPECIES........................................................................... 95
Complement Fixation Test...................................................................................................96 ID Soft™ Data Analysis Programme...................................................................................97 Positive and negative freeze-dried sera and milk................................................................98
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Ruminants
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Ruminants Akabane ID Screen® Akabane Competition Competitive ELISA for the detection of anti-G1 antibodies in ruminant serum and plasma.
High correlation with the virus neutralisation test (VNT) Sensitive and specific, with clear separation of positive and negative results Fast and convenient : ready-to-use components, with results in just over 2 hours. Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT. No cross-reactions with SBV (2) Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Competitive ELISA
Species
Ruminants
Specimens
Serum and plasma
Coated antigen
Purified virus
Conjugate
Anti-G1-HRP conjugate (concentrated 10X)
Product code
AKAC-4P
AKAC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia. (2) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.
Associated Products Akabane positive freeze-dried serum
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Negative bovine freeze-dried serum
Product Code
MRI-AKA
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Positive freeze-dried serum from an experimentally infected sheep, to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Besnoitiosis ID Screen® Besnoitia Indirect Indirect ELISA kit for the detection of anti-Besnoitia besnoiti antibodies in bovine serum or plasma.
Optimal specificity High sensitivity No sample pre-dilution required : sera are diluted directly in the ELISA plate Easy-to-use, with results in just 90 minutes
Specifications Method
Indirect ELISA
Species
Cattle
Specimens
Serum or plasma
Coated antigen
Besnoitia besnoiti purified antigen
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Product code
BSNTS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Garcia-Lunar, P. et al. An Inter-Laboratory Comparative Study of Serological Tools Employed in the Diagnosis of Besnoitia besnoiti Infection in Bovines. Transboundary and Emerging Diseases, 2012. doi:10.1111/j.1865-1682.2012.01318.x
Associated Products
Besnoitia besnoiti positive freeze-dried serum Product Code
MRI-BSNT
Format
1 ml vial (freeze-dried)
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants BHV-4 ID Screen® BHV-4 Indirect Indirect ELISA kit for the detection of antibodies against Bovine Herpes Virus type 4 (BHV-4) in bovine serum or plasma.
Accurate and reliable : clearly separates positive and negative results, with high sensitivity and specificity Fast, easy-to-use, and robust, with results in 90 minutes
Specifications Method
Indirect ELISA
Species
Cattle
Specimens
Serum or plasma
Coated antigen
BHV-4 purified antigen
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Product code
BHV4S-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Carpinschi, V, Treilles, M. (Departmental veterinary lab (LVD 50), Saint-Lo, France), Leboeuf, C. (Groupement de Défense Sanitaire, Saint-Lo, France), and Pourquier, P. (IDvet, France). Comparison of ELISA and IIF methods for the detection of BHV-4 antibodies in Bovine Serum. Poster presented at the WAVLD meeting, Madrid, 2009.
Associated Products BHV-4 monoclonal antibody
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BHV-4 positive freeze-dried serum
Product Code
MAB-BHV4
Product Code
MRI-BHV4
Format
1 ml of purified monoclonal antibody, 1mg/ml.
Format
0.5 ml vial (freeze-dried)
Description
Monoclonal antibody against Bovine Herpes Virus type 4 (BHV-4) for indirect immunofluorescence.
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control.
Bluetongue ID Screen® Bluetongue Competition Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species.
Proven specificity and sensitivity and widespread use in recent outbreaks Detection of antibodies against all BTV serotypes, at least 5-8 days after natural infection, thanks to the use of a monoclonal antibody against the highly conserved VP7 protein Easy-to-use : only one wash step, with results in 90 minutes
Specifications Method
Competitive ELISA
Species
Multiple species, including sheep, goats, cattle, buffalo, deer, and others
Specimens
Serum or plasma
Coated antigen
Recombinant VP7
Conjugate
Anti-VP7-HRP conjugate (concentrated 10X)
Product code
BTC-4P
BTC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References Vandenbussche, F., et al., Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006., Vet. Microbiol. (2007), doi:10.1016 / j.vetmic.2007.10.029.
Associated Products Bluetongue positive freeze-dried serum
Negative bovine freeze-dried serum
Product Code
MRI-BTC
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants Bluetongue ID Screen® Bluetongue Early Detection* Double antigen ELISA for the early detection of anti-VP7 antibodies in serum or plasma from ruminants and other species. Efficiently detects both IgG and IgM antibodies against all BTV serotypes.
Early identifcation of infected and vaccinated animals thanks to the efficient detection of IgM antibodies and low antibody titres (IgG or IgM). Antibodies are detected 5-8 days after natural infection, and at least 7 days postvaccination Accurate and reliable : high specificity and sensitivity for all BTV serotypes Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Double antigen ELISA
Species
Multiple species, including sheep, goat, cattle, buffalo, deer and others
Specimens
Serum or plasma
Coated antigen
Recombinant VP7
Conjugate
Recombinant VP7 antigen-HRP conjugate (concentrated 10X)
Product code
BTED-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References Oura, CAL. et al. Colostral antibody protection and interference with immunity in lambs born from sheep vaccinated with an inactivated Bluetongue serotype 8 vaccine. Vaccine (2010), doi: 10.1016/j.vaccine. 2010.01.028.
Associated Products Bluetongue positive freeze-dried serum Product Code
MRI-BTC
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
* Not available in Spain
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Negative bovine freeze-dried serum
Bluetongue ID Screen® Bluetongue Milk Indirect Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples.
The only commercial ELISA for the detection of BTV antibodies in milk Cost-effective : the use of milk samples makes sample collection easier and more economical Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serum testing
Specifications Method
Indirect ELISA
Species
Sheep, goat, cattle or buffalo
Specimens
Individual or bulk milk samples.
Coated antigen
Recombinant VP7
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Product code
BTSMILK-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References Kramps, J.A, et al., Validation of a commercial ELISA for the detection of bluetongue virus (BTV)-specific antibodies in individual milk samples of Dutch dairy cows, Vet. Microbiol. (2008), doi: 10.1016/j.vetmic.2008.01.004.
Associated Products Bluetongue positive freeze-dried milk Product Code
MRI-BTMILK
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive bovine milk to be used as internal reference material for quality control.
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Ruminants Bovine Leukosis Virus ID Screen® BLV Indirect Indirect ELISA for the detection of anti-BLV antibodies in bovine or buffalo sera and plasma (individual and pools of ten samples).
Excellent specificity and sensitivity Calibrated to detect the E05 serum standard1 Straightforward, versatile test, with short or overnight incubations for individual or pooled serum and plasma.
Specifications Method
Indirect ELISA
Species
Cattle or buffalo
Specimens
Serum and plasma (individual or pools of up to 10 samples)
Coated antigen
Purified virus
Conjugate
Anti-ruminant IgG-HRP Conjugate (concentrated 10x)
Product code
BLVS-4P
BLVS-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis. (2) European directive CEE 64/432
Associated Products BLV positive freeze-dried serum
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Negative bovine freeze-dried serum
Product Code
MRI-BLV
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Bovine Leukosis Virus ID Screen® BLV Competition Competitive ELISA kit for the detection of antibodies against the gp51 envelope protein of the Enzootic Bovine Leukosis Virus.
Excellent specificity and sensitivity Calibrated to detect the E05 serum standard1 Straightforward, versatile test, with short or overnight incubations for individual or pooled serum or plasma.
Specifications Method
Competitive ELISA
Species
Cattle or buffalo
Specimens
Serum or plasma (individual or pools of up to 10 samples)
Coated antigen
Purified virus
Conjugate
Anti-gp51-HRP Conjugate (concentrated 10x)
Product code
BLVC-4P
BLVC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis. (2) European directive CEE 64/432
Associated Products BLV positive freeze-dried serum
Negative bovine freeze-dried serum
Product Code
MRI-BLV
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Positive freeze-dried bovine serum to be used as internal reference material for quality control
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants Bovine Leukosis Virus IDvet BLV AGID Agar gel immunodiffusion (AGID) test for the detection of BLV anti-gP51 antibodies in bovine serum.
Bright, distinct precipitation lines facilitate test interpretation Standardised against the European Standard E05
Specifications Method
AGID
Species
Bovine
Specimens
Serum
Product code
BLV-AGID
BLV-AGID-NOGEL
Kit format
Includes agar gel
Agar gel not included
Reactions
240
240
Ordering information
References (1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis. (2) European directive CEE 64/432
Associated Products BLV AGID CUTTER
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Product Code
BLVCUTTER
Format
As per the European Commission Decision of 15 December 2009 amending Annex D: • Diameter of central well: 4 mm • Diameter of peripheral wells: 6 mm • Distance between central and peripheral wells: 3 mm
BovineViralDiarrhoea(BVD)/MucosalDisease(MD)/BORDERDISEASE(BD) ID Screen® BVD p80 Antigen Sandwich ELISA for the detection of the p80-125 protein of Bovine Viral Diarrhoea, Musocal Disease and Border Disease, in leukocyte fractions or ear notch samples from ruminants and all susceptible species.
Specifications Method
Sandwich ELISA
Species
Bovine, ovine, caprine, and all susceptible species
Specimens
Leukocyte fractions, ear notch samples
Coated antibody
Anti-p80/125 monoclonal antibody
Conjugate
Anti-p80/125-HRP conjugate (concentrated 10X)
Ordering information Product code
BVDAG-4P
BVDAG-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strip
References (1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
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Ruminants BovineViralDiarrhoea(BVD)/MucosalDisease(MD)/BORDERDISEASE(BD) ID Screen® BVD p80 Antibody One-Step™ One-stepTM ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum and plasma from cattle, sheep, goats and other susceptible species.
Fast and easy-to-use : only one incubation step (co-incubation of serum and conjugate), with results in under 90 minutes Accurate and reliable : high specificity and sensitivity
Specifications Method
One-StepTM ELISA
Species
Cattle, sheep, goats and other susceptible species.
Specimens
Serum or plasma
Coated antibody
Anti-p80/125 Mab
Conjugate
p80/125 antigen-HRP conjugate (concentrated 10X)
Product code
BVDOS-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
Associated Products BVD/MD/BD positive freeze-dried serum
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Product Code
MRI-BVD
Format
1 ml vial (freeze-dried)
Description
Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.
BovineViralDiarrhoea(BVD)/MucosalDisease(MD)/BORDERDISEASE(BD) ID Screen® BVD p80 Antibody Competition Fast and easy-to-use: ready-to-use conjugate and results in 90 minutes
Competitive ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum, plasma and milk from cattle, sheep, goats and other susceptible species.
Flexible : short and overnight protocols for serum, plasma or milk Applicable to multiple species, including cattle, sheep, and goats Accurate and reliable: high specificity and sensitivity Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Competitive ELISA
Species
Cattle, sheep, goats and other susceptible species.
Specimens
Individual serum and plasma samples Milk (individual samples or pools of up to 50)
Incubations
Short and overnight incubations for serum, plasma and milk
Coated antigen
p80/125 purified antigen
Conjugate
Ready-to-use anti-p80/125-HRP monoclonal antibody
Ordering information Product code
BVDC-4P
BVDC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strip
References (1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
Associated Products BVD/MD/BD positive freeze-dried serum Product Code
MRI-BVD
Format
1 ml vial (freeze-dried)
Description
Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants Brucellosis Brucellosis Antigen for the Complement Fixation Test Brucella abortus antigen for the Complement Fixation Test.
Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol.
Calibrated to give 50% haemolysis with the second international standard serum (OIESS) diluted 1/200
Ordering information Product code
AG-BRU
Kit format
100 ml
Reactions
~ 40000 reactions
References (1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
Associated Products Brucellosis positive freeze-dried serum Product Code
Product Code
MRINEG-BRU
Format
1 ml vial (freeze-dried)
Format
1 ml vial (freeze-dried)
Description
Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.
Description
Freeze-dried negative bovine serum to be used as internal reference material for quality control.
Guinea pig complement
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Brucellosis negative freeze-dried serum
MRI-BRU
Veronal buffer
Product Code
CPLT-2x5ML
Product Code
VB-4L
Format
2 x 5 ml vials
Format
Description
Freeze-dried guinea pig complement to be used in any complement fixation test (CFT).
4 vials (powder form). Reconstitute each vial with 1L of distilled water
Description
The Veronal Calcium Magnesium buffer is used to dilute sera and reagents for the complement fixation test.
Brucellosis Rose Bengal (Rapid slide agglutination antigen) Rapid slide agglutination antigen for the detection of antiBrucella abortus, melitensis, or suis antibodies in bovine, ovine, caprine, and porcine species.
Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol and coloured with rose bengal stain in an acidified buffer. Calibrated on the OIEISS International Brucella standard serum and the national secondary serum to be positive against a 1/45 dilution and negative against a 1/55 dilution, as per the European directive CEE 64/432 and OIE requirements.
Ordering information Product code
RSA-RB
Kit format
10 ml
Reactions
330 reactions (30 µl per test)
References (1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis. (2) Annex C of the European directive CEE 64/432
Associated Products Brucellosis positive freeze-dried serum
Brucellosis negative freeze-dried serum
Product Code
MRI-BRU
Product Code
MRINEG-BRU
Format
1 ml vial (freeze-dried)
Format
1 ml vial (freeze-dried)
Description
Freeze-dried highly positive bovine serum to be used as internal reference material for quality control. Positive when tested with Rose Bengal.
Description
Freeze-dried negative bovine serum to be used as internal reference material for quality control. Negative when tested with Rose Bengal.
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Ruminants Brucellosis ID Screen® Brucellosis Serum Indirect Multi-species Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).
Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum. Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples. Multi-species test for ruminants and swine
Specifications Method
Indirect ELISA
Species
Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.
Specimens
Individual serum or plasma samples, or pools of up to 10 samples
Coated antigen
Purified Brucella LPS
Conjugate
Anti-multi-species-IgG-HRP conjugate (concentrated 10X)
Product code
BRUS-MS-4P
BRUS-MS-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis. (2) Annex C of the European directive CEE 64/432
Associated Products Brucellosis positive freeze-dried serum
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Brucellosis negative freeze-dried serum
Product Code
MRI-BRU
Product Code
MRINEG-BRU
Format
1 ml vial (freeze-dried)
Format
1 ml vial (freeze-dried)
Description
Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.
Description
Freeze-dried negative bovine serum to be used as internal reference material for quality control.
Brucellosis ID Screen® Brucellosis Milk Indirect Indirect ELISA for the detection of antibodies against Brucella abortus or melitensis in bovine, ovine and caprine milk. The test may be used on individual or bulk milks*. The assessment of the diagnostic performance of this ELISA kit on field samples has shown that positive bovine milk samples may be detected in pools of up to 250 samples.
Calibrated according to the Annex C European directive CEE 64/432 and OIE requirements Straightforward test for individual or pooled milks.
* Note: The analysis of milk from small ruminants and the analysis of individual milks are not official EU tests.
Specifications Method
Indirect ELISA
Species
Ruminants
Specimens
Bovine (cattle and buffalo), ovine and caprine milk (individual or up to 250 pooled samples).
Coated antigen
Purified Brucella LPS
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Ordering information Product code
BRUMILK-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
References (1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis. (2) Annex C European directive CEE 64/432
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Ruminants Chlamydophila abortus / Enzootic abortion ID Screen® Chlamydophila abortus Indirect Multi-species This indirect multi-species ELISA detects anti-MOMP (Major Outer Membrane Protein) antibodies in ruminant serum and plasma. Please contact IDvet for use on other species.
High sensitivity and superior specificity with respect to C. pecorum thanks to the use of peptide antigens Applicable to multiple species, including sheep, goats and cattle. Please consult IDvet for use with other species
Specifications Method
Indirect ELISA
Species
Ruminants and other species
Specimens
Serum and plasma
Coated antigen
MOMP peptide
Conjugate
Anti-multi-species IgG–HRP conjugate (concentrated 10X)
Product code
CHLMS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References (1) Horigan, MW et al. (Veterinary Laboratories Agency, UK). Poster : Chlamydophila abortus – An Evaluation of Three Commercial ELISAs. Presented at the WAVLD Conference in Madrid, Spain, 2009. (2) Marques, PX et al. (University College, Dublin, Ireland). Poster: A comparison of three serological methods for the identification of pregnant ewes infected with Chlamydophila abortus. 2008. (3) Pourquier, P. (IDvet, France), Rodalakis, A and Mohamad, KY (INRA, Nouzilly, France). Preliminary validation of a new commercial ELISA kit for the detection of antibodies directed against C. abortus. Presented at the WAVLD Conference, 2007.
Associated Products Chlamydophila abortus positive freeze-dried serum
24
Product Code
MRI-CHLM
Format
1 ml vial (freeze-dried)
Description
Positive freeze-dried ovine serum to be used as internal reference material for quality control.
Interferon gamma ID Screen® Ruminant IFN-g Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g ) in activated plasma or in culture supernatant.
Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample. Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol) Practical: the standard protocol uses only 10ul per sample, meaning that less sample volume is required for activation
Specifications Method
Sandwich ELISA
Species
Bovine, ovine and caprine
Specimens
Activated plasma or culture supernatant
Coated antibody
Anti-ruminant IFN-g monoclonal antibody
Conjugate
Anti-ruminant IFN-g HRP Mab conjugate
Ordering information Product code
IFNG-2P
IFNG-4P
IFNG-10P
Kit format
2 plates
4 plates
10 plates
Reactions
192
384
960
Plate format
12 x 8-well strips
References Comtet, L., Pourquier, P. (IDvet). ID Screen® Interferon gamma ELISA: improvement of analytical sensitivity and introduction of a standard reference control to improve result interpretation. Poster presented at the 2012 International Colloquium on Paratuberculosis, Sydney, Australia.
Associated Products Reagents for in vitro cell activation Product Code
PPDPACK
Format
Reagents for 5000 reactions
Description
In vitro cell activation pack for 5000 reactions containing avian and bovine tuberculin PPD and mitogen (pokeweed). Mitogen is used as a positive activation control
Interferon gamma positive activated freeze-dried plasmas Species
Bovine
Ovine
Caprine
Buffalo
Product Code
MRI-IFNG-B
MRI-IFNG-O
MRI-IFNG-C
MRI-IFNG-BF
Format
0.5 ml vial (freeze-dried)
25
Ruminants Infectious Bovine Rhinotracheitis (IBR) ID Screen® IBR Indirect Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine serum and plasma.
Calibrated to detect the EU2 (weak-positive) standard serum 1 Straightforward and rapid, with short and overnight incubations and results in 90 minutes.
Specifications Method
Indirect ELISA
Species
Cattle and buffalo
Specimens
Serum and plasma
Coated antigen
BHV-1 purified lysat
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Product code
IBRS-4P
IBRS-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
Associated Products IBR positive freeze-dried serum
26
Negative bovine freeze-dried serum
Product Code
MRI-IBR
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Infectious Bovine Rhinotracheitis (IBR) ID Screen® IBR Milk Indirect Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine bulk milk.
Cost-effective: the use of milk samples makes sample collection easier and more economical. Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serum testing Rapid and easy-to-use Short and overnight protocols
Specifications Method
Indirect ELISA
Species
Cattle and buffalo
Specimens
Bulk milk
Coated antigen
BHV-1 purified lysat
Conjugate
Anti-ruminant IgG – HRP (concentrated 10X)
Product code
IBRMILK-4P
IBRMILK-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
27
Ruminants Infectious Bovine Rhinotracheitis (IBR) ID Screen® IBR gB Competition Competitive ELISA for the detection of anti-gB antibodies in serum and plasma.
Calibrated to detect the EU2 (weak-positive) standard serum1. Flexible test: short and overnight protocols. Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Competitive ELISA
Species
Cattle and buffalo
Specimens
Serum and plasma
Incubations
Short and overnight
Coated antigen
BHV-1 purified lysat
Conjugate
Ready-to-use anti-gB-HRP conjugate
Product code
IBRGBC-4P
IBRGBC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
Associated Products IBR positive freeze-dried serum
28
Negative bovine freeze-dried serum
Product Code
MRI-IBR
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Infectious Bovine Rhinotracheitis (IBR) ID Screen® IBR gE Competition Competitive ELISA for the detection of anti-BHV-1 gE antibodies in bovine serum, plasma and milk (individual, bulk or condensed milk samples). Detects naturally-infected animals in a vaccinated population provided that animals are vaccinated with BHV-1 gE-deleted vaccines.
May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals) Calibrated to detect the EU2 (weak-positive) standard serum1.
Specifications Method
Competitive ELISA
Species
Bovine
Specimens
Serum, plasma and milk (individual, bulk or condensed milk samples)
Coated antigen
BHV-1 purified lysat
Conjugate
Anti-gE-HRP conjugate (concentrated 10X)
Product code
IBRGEC-4P
IBRGEC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
Associated Products IBR positive freeze-dried serum
Negative bovine freeze-dried serum
Product Code
MRI-IBR
Product Code
MRINEG-BOV
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
29
Ruminants Maedi Visna Virus / Caprine Arthritis Encephalitis Virus (CAEV) ID Screen® MVV / CAEV Indirect Indirect ELISA kit for the detection of anti-Maedi Visna Virus (MVV) and anti-CAEV antibodies in ovine and caprine serum. The use of a mix of peptide antigens results in superior test performance (sensitivity, specificity, and differentiation between positive and negative results).
Reliable and accurate : clearly separates positive and negative results, with high sensitivity and specificity No non-specific reactions due to BTV-vaccination, thanks to the use of peptide antigens. Other ELISAs based on recombinant or purified viral antigens have given nonspecific reactions in BTV-vaccinated populations 1 Easy-to-use, with results in 90 minutes
Specifications Method
Indirect ELISA
Species
Sheep and goats
Specimens
Serum or plasma
Coated antigen
Mix of peptides from the GAG, TM and ENV genes.
Conjugate
Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Ordering information Screening format
Confirmation format
Product code
VISNAS-4P
Product code
VISNAB-4P
Kit format
4 plates
Kit format
4 plates (bi-well)
Reactions
384
Reactions
192
Plate format
12 x 8-well strips
Plate format
6 x 16-well strips
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
References (1) Comtet, L. (IDvet), Feliziani, F. (CEREL – IZS Umbria e Marche, Italy), Lesceu, S. (IDvet). Validation of the ID Screen® Maedi Visna Indirect ELISA: specificity on BTV8 vaccinated sheep and detection of seroconversion. Poster presented at the 2010 EAVLD meeting, Lelystad, Holland.
Associated Products MVV / CAEV positive freeze-dried serum
30
Product Code
MRI-VISNA / CAEV
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive ovine and caprine serum pool to be used as internal reference material for quality control.
Neospora caninum ID Screen® Neospora caninum Indirect Multi-species Indirect multi-species ELISA kit for the detection of antiNeospora caninum antibodies in serum or milk.
Cost-effective method for disease monitoring, with high specificity and sensitivity Fast and convenient protocol with results in 60 minutes and protocols for both serum and milk samples Applicable to multiple species, including ruminants and dogs.
Specifications Method
Indirect ELISA
Species
Multiple species, including ruminants and dogs.
Specimens
Serum, plasma or milk
Coated antigen
Purified Neospora caninum extract
Conjugate
Anti-multi-species IgG –HRP conjugate (concentrated 10X)
Product code
NCS-MS-2P
NCS-MS-5P
Kit format
2 plates
5 plates
Reactions
192
480
Plate format
12 x 8-well strips
Ordering information
References Alvarez-García, G., et al., Serological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests. Vet. Parasitol. (2013), http://dx.doi. org/10.1016/j.vetpar.2013.07.033
Associated Products
N. caninum positive freeze-dried serum
Negative bovine freeze-dried serum
Product Code
MRI-NC
Product Code
MRINEG-BOV
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
31
Ruminants Parainfluenza 3 PI-3 monoclonal antibody
32
Product code
MAB-PI3
Description
Monoclonal antibody against Parainfluenza-3 virus for indirect immunofluorescence
Format
1 ml of purified monoclonal antibody, 1mg/ml
Paratuberculosis / Johne’s Disease ID Screen® Paratuberculosis Indirect Indirect absorbed(3) ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.
Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results Automatable and rapid : results in 90 minutes. Screening and confirmation formats available Short or overnight incubations
Specifications Method
Indirect absorbed ELISA
Species
Ovine, caprine, bovine (cattle or buffalo)
Specimens
Serum, plasma or bovine milk
Coated antigen
Purified MAP extract
Conjugate
Anti-ruminant-HRP conjugate (concentrated 10X)
Incubations
Short or overnight
Ordering information Confirmation format*
Screening format Product code
PARAS-4P
PARAS-10P
Product code
PARAB-4P
Kit format
4 plates
10 plates
Kit format
4 plates (bi-well)
Reactions
384
960
Reactions
192
Plate format
12 x 8-well strips
Plate format
6 x 16-well strips
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).
References (1) Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008). (2) Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008). (3) OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
Associated Products Mycobactine J
Paratuberculosis negative freeze-dried serum
Product Code
MYCO
Paratuberculosis positive freeze-dried serum
Format
2 mg vial (freeze-dried)
Product Code
MRI-PARA
Product Code
MRINEG-PARA
Description
Ferric Mycobactin J is an iron-chelated substance used as a growth factor for the isolation of mycobactin-dependent Mycobacterium.
Format
1 ml vial (freeze-dried)
Format
2 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Freeze-dried negative bovine serum to be used as internal reference material for quality control.
33
Ruminants Peste des Petits Ruminants ID Screen® PPR Competition Competitive ELISA kit for the detection of anti-PPRV nucleoprotein (NP) antibodies in sheep and goat serum or plasma. Please contact IDvet for use on other species. The only commercial ELISA approved by the FAO / OIE CIRAD laboratory, and based on the CIRAD biological components (antigen and conjugate). This test does not contain any virus particles.
Convenient format: The only commercial ELISA to supply ready-to-use, coated plates, and all other components in liquid format. Plates are supplied in strips Straightforward and rapid protocol : each sample is deposited only once (screening format), and results are obtained in 90 minutes High specificity and sensitivity
Specifications Method
Competitive ELISA
Species
Multiple species, including sheep, goats and others
Specimens
Serum or plasma
Coated antigen
Recombinant PPRV nucleoprotein (NP)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
PPRC-4P
PPRC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References . ibeau, G., Préhaud, C., Lancelot, R., Colas, F., Guerre, L., Bishop, D.H., Diallo, A. Development of a competitive ELISA for detecting antibodies to the Peste des Petits L Ruminants virus using a recombinant nucleoprotein. Res Vet Sci. 1995 Jan;58(1):50-5.
Associated Products PPR positive freeze-dried serum
34
Product Code
MRI-PPR
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive caprine serum to be used as internal reference material for quality control.
Peste des Petits Ruminants ID Screen® PPR Antigen Capture Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis. Based on reagents developed at a FAO/OIE PPRV reference laboratory (CIRAD, Montpellier, France). IDvet contributed to the improvement of this test by optimizing test performance and component stability. This test does not contain any virus particles.
Equivalent performance to RT-PCR 7 Improved sensitivity for samples with weak or strong PPRV titres Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC. Convenient and easy-to-use: Microplates are supplied coated in strips of 8 wells. Straightforward and rapid protocol, with results in 90 minutes
Specifications Method
Sandwich ELISA
Species
Ovine and caprine
Specimens
Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Coated antibody
Anti-PPRV NP antibody
Conjugate
Anti-PPRV NP Mab–HRP conjugate (concentrated 10X)
Product code
PPRAG-1P
PPRAG-2P
Kit format
1 plate
2 plates
Reactions
96
192
Plate format
12 x 8-well strips
Ordering information
References (1).O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231 (2).Recognizing peste des petits ruminants - A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR (3).Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304 (4).Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108 (5).Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RTPCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335 (6).Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plantdiseases/serodiagnosis-of-peste-des-petits-ruminants-virus (7) Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.
Associated Products Freeze-dried PPR Antigen Product Code
MRI-PPRAG
Format
1 ml vial (freeze-dried)
Description
Freeze-dried inactivated PPR antigen to be used as internal reference material for quality control.
35
Ruminants
36
Q Fever ID Screen® Q Fever Indirect Multi-species Indirect multi-species ELISA for the detection of anti-Coxiella burnetii antibodies in serum and plasma or in individual or bulk milk samples from multiple species.
Applicable to multiple species, including cattle, sheep and goats. Please contact IDvet for use in other species. Straightforward, easy-to-use, with results in 90 minutes
Specifications Method
Indirect ELISA
Species
Multiple species, including cattle, sheep, goats and others
Specimens
Serum, plasma or milk
Coated antigen
Inactivated Coxiella burnetii antigen from a bovine isolate
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code
FQS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
Associated Products Q Fever positive freeze-dried serum Product Code
MRI-FQ
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control
37
Ruminants Rift Valley Fever ID Screen® Rift Valley Fever Competition Multi-species Competitive ELISA kit for the detection of anti-Rift Valley Fever (RVFV) nucleoprotein antibodies in ruminant serum or plasma.
Excellent analytical sensitivity, sensitivity and specificity, as proven in recent outbreaks
This test does not contain any virus particles.
Multi-species test : may be used on ruminants, horses, dogs and other species Convenient and rapid format: ready-to-use, coated plates in strips, and all other components in liquid format. Results in less than 2 hours Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Competitive ELISA
Species
Multiple species, including ruminants, camels, horses, dogs and others
Specimens
Serum or plasma
Coated antigen
RVF Virus recombinant nucleoprotein (NP)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Ordering information Product code
RIFTC-4P
RIFTC-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
References (1) Comtet, L. et Pourquier, P. (IDvet), Marié, J-L. & Davoust, B. (French Defence Medical Service, Working group of animal epidemiology, Marseille, France), CêtreSossah, C. (CIRAD, Montpellier, France). Preliminary validation of the ID Screen® Rift Valley Fever Competition Multi-species ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands. (2) Ahmed B. et al. Unexpected Rift Valley Fever Outbreak, Northern Mauritania. Emerging Infectious Diseases. Vol. 17, No. 10. October 2011. (3) Kortekaas, J. et al. European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus. Journal of Virological Methods. September 2012. (4) Antonis, A.F.G. et al. Vertical Transmission of Rift Valley Fever Virus Without Detectable Maternal Viremia. Vector-borne and zoonotic diseases. Vol. 13, No. X. May 2013.
Associated Products Rift Valley Fever IgG-positive freeze-dried serum
38
Product Code
MRI-RIFT
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control
Rift Valley Fever ID Screen® Rift Valley Fever IgM Capture IgM Antibody Capture (IMAC) ELISA for the detection of antinucleoprotein (NP) IgM antibodies in ruminant serum and plasma. The detection of anti-IgM antibodies indicates recent infection.
Accurate and reliable : excellent specificity and analytical and diagnostic sensitivity Convenient format: Ready-to-use, coated plates in strips, and all other components in liquid format.
This test does not contain any virus particles.
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
IMAC (IgM Antibody Capture) ELISA
Species
Ruminants
Specimens
Serum or plasma
Coated antibody
Anti-bovine-ovine-caprine IgM antibody
Antigen
RVFV recombinant nucleoprotein (freeze-dried)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Protocol*
(1) Sample Incubation (2) Three washes (3) RVF nucleoprotein incubation (4) Three washes (5) Anti-NP-HRP conjugate incubation (6) Three washes (7) Substrate Incubation
* Each sample is tested in duplicate in an antigen-coated well (even columns) and in an adjacent noncoated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
Ordering information Product code
RIFTIGM-4P
RIFTIGM-10P
Kit format
4 plates (biwell format*)
10 plates (biwell format*)
Reactions
192
480
Plate format
6 x 16 well strips
Associated Products Rift Valley Fever IgM-positive freeze-dried serum Product Code
MRI-RIFTIGM
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried anti-RVFV recombinant NP ovine serum to be used as internal reference material for quality control
39
Ruminants Schmallenberg virus ID Screen® Schmallenberg virus Indirect Multi-species Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum and plasma from multiple species, including cattle, sheep and goats. Please contact IDvet for use on other species.
The first commercial ELISA for SBV serology High sensitivity and specificity in external validation studies Approved by the ANSES (France) and FLI (Germany) laboratories Automatable and therefore suited to high throughput testing Screening and confirmation formats available
Specifications Method
Indirect ELISA
Species
Ruminants and other species
Specimens
Serum, plasma
Coated antigen
Recombinant SBV nucleoprotein antigen
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Ordering information Screening format
Confirmation format*
Product code
SBVS-2P
Kit format
2 plates
4 plates
Kit format
4 plates (bi well)
10 plates (bi well)
Reactions
192
384
Reactions
192
480
Plate format
12 x 8-well strips
Plate format
6 x 16 well strips
SBVS-4P
Product code
SBVB-4P
SBVB-10P
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
References (1) Jack C., Anstaett O, Adams J, Noad R, Browlie J., Mertens P. Evidence of seroconversion to SBV in camelids. Veterinary Record 2012 170:603 doi: 10.1136/vr.e3939 (2) Kaba et al. Schmallenberg Virus Antibodies Detected in Poland. Transboundary and Emerging Diseases. Rapid Communication. 2012. (3) Linden A, Desmecht D, Volpe R, Wirtgen M, Pirson J, Paternostre J, et al. Epizootic Spreads of Schmallenberg virus among wild cervids, Belgium, fall 2011. Emerg Infect Dis (Internet). 2012 Dec. (4) Bréard E, Lara E, Comtet L, Viarouge C, Doceul V, et al. (2013) Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies. PLoS ONE 8(1): e53446. doi:10.1371/journal.pone.0053446
Associated Products Schmallenberg virus positive freeze-dried serum
40
Negative bovine freeze-dried serum
Product Code
MRI-SBV
Product Code
MRINEG-BOV
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Schmallenberg virus ID Screen® Schmallenberg virus Competition Multi-species Competitive ELISA for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum or plasma from multiple-species .
The only competitive ELISA for SBV serology Applicable to multiple species, including cattle, sheep and goats Improved sensitivity for sera with low VNT titres Earlier detection of seroconversion Improved specificity Robust and easy-to-use: the conjugate is supplied ready-to-use, and results are obtained in 90 minutes
Specifications Method
Competitive ELISA
Species
Ruminants and other species
Specimens
Serum and plasma
Coated antigen
Recombinant SBV nucleoprotein antigen
Conjugate
Ready-to-use anti-SBV nucleoprotein-HRP conjugate
Ordering information Product code
SBVC-2P
SBVC-4P
Kit format
2 plates
4plates
Reactions
192
384
Plate format
12 x 8 well strips
References (1) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.
Associated Products Schmallenberg virus positive freeze-dried serum
Negative bovine freeze-dried serum
Product Code
MRI-SBV
Product Code
MRINEG-BOV
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Description
Negative freeze-dried bovine serum to be used as internal reference material for quality control.
41
Ruminants Schmallenberg virus ID Screen® Schmallenberg virus Milk Indirect Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in ruminant individual and bulk milks.
The only commercial ELISA for the detection of SBV NP antibodies in milk Ideal for monitoring the spread of the virus in disease-free regions Cost-effective: the use of milk samples makes sample collection easier and more economical. Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serum testing
Specifications Method
Indirect ELISA
Species
Bovine, ovine and caprine
Specimens
Individual or bulk milks
Coated antigen
Recombinant SBV antigen
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Ordering information
42
Product code
SBVMILK-2P
SBVMILK-4P
Kit format
2 plates
4plates
Reactions
192
384
Plate format
12 x 8 well strips
Toxoplasmosis ID Screen® Toxoplasmosis Indirect Multi-species Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, and cats. Please contact IDvet for use with other species.
Easy-to-use, with results in 90 minutes Versatile protocol for serum, plasma or meat juice, and applicable to multiple species, including ruminants, swine, dogs and cats
Specifications Method
Indirect ELISA
Species
Ruminants, swine, dogs and cats.
Specimens
Serum, plasma or meat juice
Coated antigen
Toxoplasma gondii P30 antigen
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code
TOXOS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Sheep (1) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008 (2) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009. Swine (3) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara. (4) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009. (5) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011. Deer (6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010. (7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009. Dogs and cats (8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009. Wild & domestic animals (9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.
43
al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris ry Research 2011, 42:24. Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 petitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.
., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), etmic.2010.08.014.
t al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human s. Vet. Res (2010) 41:74. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology 9-2123.
tis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
44
EQUINE
45
EQUINE Equine Infectious Anemia ID Screen® Equine Infectious Anemia Double Antigen Double antigen ELISA kit for the detection of antibodies directed against the p26 protein of the Equine Infectious Anemia Virus (EIAV) in horse serum and plasma.
Highly sensitive and specific Calibrated to detect the OIE standard positive antiEIAV serum Fast and easy-to-use, with results in 90 minutes
Specifications Method
Double antigen ELISA
Species
Equine
Specimens
Serum and plasma
Coated antigen
p26 recombinant antigen
Conjugate
p26 antigen-HRP conjugate (concentrated 10X)
Product code
EIAED-1P
EIAED-4P
Kit format
1 plate
4 plates
Reactions
96
384
Plate format
12 x 8-well strips
Ordering information
References Pourquier, P. et al. Validation of the ID Screen® Equine Infectious Anemia Double Antigen ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.
Associated Products EIA positive freeze-dried serum
46
Product Code
MRI-EIA
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive horse serum to be used as internal reference material for quality control.
Equine Infectious Anemia IDvet EIA AGID Agar gel immunodiffusion (AGID) test for the detection of EIA anti-p26 antibodies in equine serum.
Clear, bright lines for easy reading OIE-prescribed protocol1 24-month shelf-life May be purchased with or without agar gel (the IDvet agar gel allows for clearer precipitation lines)
Specifications Method
AGID (Coggins-test)
Species
Equine
Specimens
Serum
Antigen
EIAV p26
Product code
EIA-AGID
EIA-AGID-NOGEL
Kit format
Includes agar gel
Agar gel not included
Reactions
200
200
Ordering information
References OIE Terrestrial Manual 2013; 2.5.6; Equine Infectious Anemia
Associated Products EIA AGID CUTTER Product Code
EIACUTTER
Format
As per OIE specifications: • A seven-well pattern is used with one centre well encircled by 6 wells • The wells are 2.4 mm apart and 5.3 mm in diameter
47
EQUINE
48
Equine Viral Arteritis ID Screen® Equine Viral Arteritis Indirect* Indirect confirmation ELISA kit for the detection of anti-EVA antibodies in horse serum and plasma.
Compatible with cytotoxic sera1 which cannot be tested with the virus neutralization test (VNT) Easy-to-use, with results in just over 2 hours
Specifications Method
Indirect ELISA
Species
Equine
Specimens
Serum and plasma
Coated antigen
Specific EVA peptide
Conjugate
Anti-horse IgG-HRP conjugate (concentrated 10X)
Product code
EVAB-4P
Kit format
4 plates (Biwell format)*
Reactions
192
Plate format
6 x 16-well strips
Ordering information
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
References (1) Legrand, L., Pitel, P.H., Fotier, G., Pronost, S. Testing for antibodies to equine arteritis virus. The Veterinary Record, April 4th, 2009.
Associated Products EVA positive freeze-dried serum Product Code
MRI-EVA
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive horse serum to be used as internal reference material for quality control.
* Not available in France 49
EQUINE Influenza A ID Screen® Influenza A Antibody Competition Multi-species Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants in multiple species thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
Highly sensitive and specific Convenient multi-species test applicable to birds, horses, swine and others Easy-to-use, with results in under 2 hours
Specifications Method
Competitive ELISA
Species
Multiple species, including horses, birds, swine and others.
Specimens
Serum and plasma
Coated antigen
Influenza A nucleoprotein (NP)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
FLUACA-2P
FLUACA-4P
FLUACA-10P
Kit format
2 plates
4 plates
10 plates
Reactions
192
384
960
Plate format
12 x 8-well strips
Ordering information
References Horses: (1) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014. Birds: (2) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24. (3) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland. (4) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (5) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy. Swine : (6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123. Dogs: (8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
50
Influenza A ID Screen® Influenza A Antigen Capture Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
High sensitivity and specificity Cost-effective for the analysis of large sample numbers Applicable to multiple species, including horses, birds and swine - please contact IDvet for more information Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests Easy-to-use and rapid, with results in under 1 hour
Specifications Method
Sandwich ELISA
Species
Avian, porcine, equine
Specimens
-Broncho-pulmonary liquids from pigs, horses and birds. -Bird cloacae swab samples. -Bird faeces.
Coated antibody
Anti-nucleoprotein (NP) monoclonal antibody
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
INFLAG-2P
Kit format
2 plates
Reactions
192
Plate format
96 individual wells
Ordering information
51
EQUINE Strangles / Streptococcus equi ID Screen® Streptococcus equi Indirect Indirect ELISA kit for the detection of anti-Streptococcus equi in equine serum and plasma.
Easy-to-use, standardized assay, with results in just over 2 hours Qualitative protocol to detect previous exposure to S. equi Quantitative protocol to assess SeM-specific antibody levels (horses with high SeM-specific serum antibodies may be predisposed to purpura when vaccinated for S. equi subsp equi with an attenuated live intranasal S. equi vaccine 1,2,3). Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Indirect ELISA
Species
Horses
Specimens
Serum and plasma
Coated antigen
Recombinant SeM
Conjugate
Anti-horse IgG-HRP conjugate (concentrated 10X)
Product code
STREPTOS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References (1) Boyle, Ashley G. Streptococcus equi subspecies equi Infection (Strangles) in Horses. Vetlearn.com March 2011. Compendium: Continuing education for veterinarians. E1-8. (2) Sweeney CR, Timoney JF, Newton JR, Hines MT. Streptococcus equi infections in horses: guidelines for treatment, control and prevention of strangles. J Vet Intern Med 2005:19:123-134. (3) Timoney, JF. Equine strangles. Proc 45th Annu Meet AAEP 1999:31-37.
Associated Products Streptococcus equi positive freeze-dried serum
52
Product Code
MRI-STREPTO
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive horse serum to be used as internal reference material for quality control.
Trichinellosis ID Screen® Trichinella Indirect Multi-species Indirect multi-species ELISA kit for the detection of antiTrichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use in other species.
Highly sensitive and specific Applicable to multiple species, including pigs, wild boar and horses Serum, plasma and meat juice protocols
Specifications Method
Indirect ELISA
Species
Multiple species, including pigs , wild boar, horses and others
Specimens
Serum, plasma or meat juice
Coated antigen
Trichinella E/S antigen
Conjugate
Anti-multi-species-HRP conjugate (concentrated 10X)
Ordering information Product code
TRICHIS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
53
EQUINE West Nile Virus ID Screen® West Nile Competition Multi-species Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit crossreacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
Highly sensitive and specific detection of WNV antibodies Applicable to multiple species, including horses, birds and others. Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
Specifications Method
Competitive ELISA
Species
Equine & avian – please contact IDvet for use on other species
Specimens
Serum or plasma
Coated antigen
pr-E antigen
Conjugate
Anti-pr-E-HRP conjugate (concentrated 10X)
Product code
WNC-1P
WNC-2P
Kit format
1 plate
2 plates
Reactions
96
192
Plate format
12 x 8-well strips
Ordering information
References
(1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever. Horses: (2) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France. (3) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007. (4) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101. Birds: (5) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009. (6) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011. (7) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012), http://dx.doi.org/10.1016/j.vetmic.2012.07.041. (8) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journal of Animal and Veterinary Advances 11 (13):221-2214,2012 Other species: (9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.
Associated Products West Nile IgG positive freeze-dried serum
54
Product Code
MRI-WN
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive horse serum to be used as internal reference material for quality control.
West Nile Virus ID Screen® West Nile IgM Capture IgM Antibody Capture ELISA (IMAC) kit for the detection of antipr-E IgM antibodies in horse serum and plasma. The detection of IgM antibodies indicates recent infection and WNV circulation. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection Rapid and convenient : all reagents are supplied readyto-use, samples are diluted directly in the plate (no predilutions required), and all reagents may be transported at room temperature and stored at 4°C.
Specifications Method
IMAC (IgM Antibody Capture) ELISA
Species
Equine
Specimens
Serum and plasma
Coated antibody
Anti-horse IgM polyclonal antibody
Antigen
Ready-to-use WNV pr-E antigen
Conjugate
Anti-WNV pr-E HRP conjugate (10X)
Protocol*
(1) Sample Incubation (2) Three washes (3) WNV ready-to-use antigen incubation (4) Anti-WNV prE-HRP conjugate incubation (5) Three washes (6) Substrate Incubation: 15 min
* Samples are deposited in duplicate in adjacent even and odd-numbered wells; the WNV pr-E is added only to even-numbered wells. Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
Ordering information Product code
WNIGM-1P
WNIGM-4P
Kit format
1 plate (biwell format*)
4 plates (biwell format*)
Reactions
48
192
Plate format
6 x 16-well strips
References (1) .Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156 (2) OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.
Associated Products West Nile IgM positive freeze-dried serum Product Code
MRI-WNIGM
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried IgM-positive horse serum to be used as internal reference material for quality control.
55
AVIAN
57
AVIAN Infectious Bronchitis ID Screen® Infectious Bronchitis Indirect Indirect ELISA for the detection of antibodies against the Infectious Bronchitis Virus (IBV) in chicken sera.
Detects all IBV variants
May be used to detect infection or to quantify IBV antibody levels in vaccinated birds.
High specificity and very low background
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
Short incubation times: results in 75 minutes (30-30-15 protocol) Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken
Specimens
Serum
Coated antigen
IBV purified antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Ordering information Product code
IBVS-5P
IBVS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Associated Products IBV positive freeze-dried serum
58
SPF negative freeze-dried serum
Product Code
MRI-IBV
Product Code
MRINEG-BIRD
Format
0.25 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Infectious Bronchitis ID Screen® Infectious Bronchitis Competition Competitive ELISA kit for the detection of anti-IBV NP antibodies in chicken serum and egg yolk.
High sensitivity and specificity Protocols for serum and egg yolk Earlier detection of infected animals The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Competitive ELISA
Species
Chickens
Specimens
Serum or egg yolk
Coated antigen
IBV purified antigen
Conjugate
Anti-IBV-HRP conjugate (concentrated 10X)
Ordering information Product code
IBVC-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Associated Products IBV positive freeze-dried serum
SPF negative freeze-dried serum
Product Code
MRI-IBV
Product Code
MRINEG-BIRD
Format
0.25 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
59
AVIAN Infectious BURSAL DISEASE / GUMBORO DISEASE ID Screen® IBD Indirect Indirect ELISA for the detection of antibodies against the Infectious Bursal Disease Virus (IBDV) in chicken sera. May be used to monitor the flock’s immune status, to quantify IBD antibody levels pre- and post-vaccination, or to determine the best time for vaccination by detecting maternal antibodies.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
High specificity and very low background Short incubation times: results in 75 minutes (30-30-15 protocol) Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken
Specimens
Serum
Coated antigen
IBD purified antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Ordering information Product code
IBDS-5P
IBDS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Associated Products IBD positive freeze-dried serum
60
SPF negative freeze-dried serum
Product Code
MRI-IBDS
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Infectious BURSAL DISEASE / GUMBORO DISEASE ID Screen® IBD VP2 Indirect ELISA for the detection of anti-IBDV VP2 antibodies in chicken sera. May be used to quantify IBD antibody levels pre- and postvaccination. This test is designed to be used with animals vaccinated with recombinant VP2 vector vaccines.
May be used with animals vaccinated with recombinant VP2 vector vaccines High specificity and very low background Short incubation times: results in 75 minutes (30-30-15 protocol) Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken
Specimens
Serum
Coated antigen
Recombinant VP2 antigen
Conjugate
Anti-immunoglobulin-HRP conjugate (concentrated 10X)
Product code
IBDVP2-5P
IBDVP2-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products IBD VP2 positive freeze-dried serum
SPF negative freeze-dried serum
Product Code
MRI-IBDVP2
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried VP2 positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
61
AVIAN Infectious LARYNGOTRACHEITIS (ILT) ID Screen® ILT Indirect Indirect ELISA for the detection of antibodies directed against the avian Infectious Laryngotracheitis Virus (ILTV) in vaccinated and/or infected chicken sera.
High specificity and analytical sensitivity Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chickens
Specimens
Serum
Coated antigen
ILTV purified antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10x)
Ordering information Product code
ILTS-5P
ILTS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Associated Products ILT positive freeze-dried serum
62
SPF negative freeze-dried serum
Product Code
MRI-ILT
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Influenza A ID Screen® Influenza A Antibody Competition Multi-species Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
Highly sensitive and specific Convenient multi-species test applicable to birds, horses, swine and other species - please contact IDvet for more information Easy-to-use, with results in under 2 hours
Specifications Method
Competitive ELISA
Species
Multiple species, including birds, horses, swine and others
Specimens
Serum and plasma
Coated antigen
Influenza A nucleoprotein (NP)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
FLUACA-2P
FLUACA-4P
FLUACA-10P
Kit format
2 plates
4 plates
10 plates
Reactions
192
384
960
Plate format
12 x 8-well strips
Ordering information
References Birds: (1) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24. (2) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland. (3) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (4) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy. Horses: (5) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014. Swine : (6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123. Dogs: (8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
Associated Products Influenza A positive freeze-dried serum
SPF negative freeze-dried serum
Product Code
MRI-FLUACA/N1
Product Code
MRINEG-BIRD
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
63
AVIAN Influenza A ID Screen® Influenza subtype-specific kits Range of competitive ELISA kits for the detection of subtypespecific Influenza antibodies in avian serum or plasma.
Name
Product code
ID Screen Influenza N1 Antibody Competition
FLUACN1-2P
ID Screen Influenza N2 Antibody Competition
FLUACN2-2P
ID Screen® Influenza N9 Antibody Competition
FLUACN9-2P
ID Screen® Influenza H5 Antibody Competition
FLUACH5-2P
ID Screen® Influenza H7 Antibody Competition
FLUACH7-2P
®
®
May be used for DIVA diagnostics (Differentiation of Infected and Vaccinated Animals)* Applicable to both domestic & wild avian species High sensitivity and specificity : validated by OIE labs, with excellent results Easy-to-use, with results in under 2.5 hours * For example, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain, but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype as evidence of natural infection.
Specifications Method
Competitive ELISA
Species
Avian
Specimens
Serum or plasma
Coated antigen
Purified native inactivated subtype-specific antigen
Conjugate
Anti-subtype-HRP Mab conjugate (concentrated 10X)
Ordering information Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
References (1) Dundon, W.G,. Terregino, C., Tuttoilmondo, V., Pizzuto, M., Busani, L., Mancin, M., Cattoli, G., Capua, I. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Poster presented at EPIZONE 2007, Poland. (2) Bötcher, L., Schöttker-Wegner, H.H., Nöckler, A. Summary of the German presentation at the AVID Meeting (September 15-17, 2010). Use of an H5-antibody ELISAs for the serological differentiation of AIV-antibodies. Niedersächsisches Landesamt für Verbrauchschutz und Lebensmittelsicherheit Veterinärinstitut, Oldenburg, Germany. (3) Robles, F. et al. Implementation of « DIVA » Concept with specific ELISA kits ; When Subunit H5 Avian Influenza Vaccine is Used. World Academy of Science, Engineering and Technology 65 2010.
Associated Products Influenza N1 positive freeze-dried chicken serum* Product Code
Product Code
MRI-FLUACH7
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
SPF negative freeze-dried chicken serum*
64
Influenza H7 positive freeze-dried chicken serum*
MRI-FLUACA/N1
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
* Freeze-dried chicken sera to be used as internal reference material for quality control.
Influenza A ID Screen® Influenza A Antigen Capture Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
High sensitivity and specificity Cost-effective for the analysis of large sample numbers Applicable to multiple species, including birds, horses and swine - please contact IDvet for more information Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests Easy-to-use and rapid, with results in under 1 hour
Specifications Method
Sandwich ELISA
Species
Multiple species, including birds, pigs, horses and others.
Specimens
-Broncho-pulmonary liquids from pigs, horses and birds. -Bird cloacae swab samples. -Bird faeces.
Coated antibody
Anti-nucleoprotein (NP) monoclonal antibody
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
INFLAG-2P
Kit format
2 plates
Reactions
192
Plate format
96 individual wells
Ordering information
Associated Products SPF negative freeze-dried serum Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
65
AVIAN AVIAN MYCOPLASMOSIS ID Screen® Mycoplasma gallisepticum Indirect Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum antibodies in chicken and turkey sera to monitor the flock’s immune status.
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity No cross-reactions with other mycoplasmas
Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
Short incubation times: results in 75 minutes (30-30-15 protocol) Applicable to chickens and turkeys Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls Detects anti-MG antibodies further to vaccination with the 6/85 strain The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken and turkey
Specimens
Serum
Coated antigen
Recombinant MG antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Product code
MG-5P
MG-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products MG positive freeze-dried serum
66
SPF negative freeze-dried serum
Product Code
MRI-MG
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
AVIAN MYCOPLASMOSIS ID Screen® Mycoplasma synoviae Indirect Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status.
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity No cross-reactions with other mycoplasmas
Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
Short incubation times: results in 75 minutes (30-30-15 protocol) Applicable to chickens and turkeys Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken and turkey
Specimens
Serum
Coated antigen
Recombinant MS antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Product code
MS-5P
MS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products MS positive freeze-dried serum
SPF negative freeze-dried serum
Product Code
MRI-MS
Product Code
MRINEG-BIRD
Format
1 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
67
AVIAN AVIAN MYCOPLASMOSIS ID Screen® MG/MS Indirect Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status. Positive results should be confirmed using the separate ID Screen® MG or MS Indirect ELISAs, and then by other methods such as Rapid Slide Agglutination (RSA).
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity No cross-reactions with other mycoplasmas Short incubation times: results in 75 minutes (30-30-15 protocol) Applicable to chickens and turkeys
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken and turkey
Specimens
Serum
Coated antigen
MG and MS recombinant antigens
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Product code
MG/MS-5P
MG/MS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products MG positive serum
68
Product Code
MRI-MG
Format Description
MS positive serum
SPF negative serum
MRI-MS
Product Code
MRINEG-BIRD
0.5 ml vial (freeze-dried) Format
1 ml vial (freeze-dried)
Format
1 ml vial (freeze-dried)
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Product Code Description
69
AVIAN Newcastle Disease ID Screen® Newcastle Disease Indirect Indirect ELISA for the detection of antibodies against the Newcastle Disease Virus (NDV) in chicken sera. May be used to detect infection or to quantify NDV antibody levels in vaccinated birds.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
High specificity and very low background Short incubation times: results in 75 minutes (30-30-15 protocol) Excellent correlation with hemagglutination inhibition (HI) Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken
Specimens
Serum
Coated antigen
NDV purified antigen
Conjugate
Anti-chicken-HRP conjugate (concentrated 10X)
Product code
NDVS-5P
NDVS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products NDV positive freeze-dried serum
70
SPF negative freeze-dried serum
Product Code
MRI-NDV
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
NEWCASTLE DISEASE ID Screen® Newcastle Disease Competition Competitive ELISA kit for the detection of anti-NDV nucleoprotein antibodies in serum and egg yolk (domestic and wild avian species). Applicable to both domestic and wild avian species, this test may be used to determine the immune status of individuals, flocks or populations.
Multi-species test: the only commercial ELISA applicable to both domestic and wild avian species High sensitivity and specificity: • Earlier detection of seroconversion (7 dpi) compared with indirect ELISAs • High analytical sensitivity • Detection of all strains • No cross-reactions with Avian Paramyxoviruses 3 (APMV-3). Flexible and rapid protocol : applicable to serum, plasma and egg yolk samples, and results are obtained in just 70 minutes. Used worldwide in government monitoring programs The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Competitive ELISA
Species
Avian (domestic and wild species)
Specimens
Serum, plasma and egg yolk
Coated antigen
NDV-purified antigen
Conjugate
Anti-NDV-HRP conjugate (concentrated 10X)
Product code
NDVC-2P
NDVC-5P
NDVC-10P
Kit format
2 plates
5 plates
10 plates
Reactions
192
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products NDV positive freeze-dried serum
SPF negative freeze-dried serum
Product Code
MRI-NDV
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
71
AVIAN SALMONELLOSIS ID Screen® Avian Salmonella Indirect – Groups B & D Indirect ELISA for the detection of anti-Salmonella group B and D antibodies in chicken sera.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
Very low background Detects antibodies directed against Salmonella enteritidis (Se), S. thyphimurium (St), and the other avian salmonellas belonging to groups B and D. Short incubation times with results in 75 minutes Comprehensive data analysis with the IDSoft™ program Freeze-dried positive and negative reference sera available as internal controls The stop solution no longer contains H2SO4, meaning that it is less corrosive
Specifications Method
Indirect ELISA
Species
Chicken
Specimens
Serum
Coated antigen
Purified Salmonella LPS
Conjugate
Anti-chicken IgG-HRP conjugate (concentrated 10X)
Product code
SALS-5P
SALS-10P
Kit format
5 plates
10 plates
Reactions
480
960
Plate format
12 x 8-well strips
Ordering information
Associated Products SAL positive freeze-dried serum
72
SPF negative freeze-dried serum
Product Code
MRI-SALS
Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
West Nile Virus ID Screen® West Nile Competition Multi-species Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit crossreacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
Highly sensitive and specific detection of WNV antibodies Multi-species test for horses and birds Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
Specifications Method
Competitive ELISA
Species
Multiple species, including birds, horses, and others.
Specimens
Serum or plasma
Coated antigen
pr-E antigen
Conjugate
Anti-pr-E-HRP conjugate (concentrated 10X)
Product code
WNC-1P
WNC-2P
Kit format
1 plate
2 plates
Reactions
96
192
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever. Birds: (2) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009. (3) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011. (4) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012), http://dx.doi.org/10.1016/j.vetmic.2012.07.041. (5) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journal of Animal and Veterinary Advances 11 (13):221-2214,2012 Horses: (6) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France. (7) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007. (8) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101. Other species: (9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.
Associated Products SPF negative freeze-dried serum Product Code
MRINEG-BIRD
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
73
74
SWINE
75
SWINE Actinobacillus pleuropneumoniae ID Screen® APP Screening Indirect (serotypes 1 through 12) Indirect ELISA kit for the detection of antibodies against APP serotypes 1 through 12 in swine serum, plasma and meat juice.
Screening of 12 APP serotypes in a single test. Serotyping may be performed on positive samples using the ID Screen® serotyping ELISAs (opposite page). Fast and easy-to-use, no sample pre-dilution required, and results in just 75 minutes The same straightforward protocol is used for all ID Screen® APP ELISAs, making it easier to use several tests at once Serum, plasma and meat juice protocols
Specifications Method
Indirect ELISA
Species
Swine
Specimens
Serum, plasma or meat juice
Coated antigen
APP antigens
Conjugate
Anti-swine IgG–HRP conjugate (concentrated 10X)
Product code
APPS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.
Associated Products APP positive freeze-dried sera Product Code
MRI-APP-1-9-11
MRI-APP-2
MRI-APP-3-8
MRI-APP-4-7
MRI-APP-5
MRI-APP-6
MRI-APP-10
MRI-APP-12
Format
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freezedried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
APP negative freeze-dried serum
76
Product Code
MRINEG-APP
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried negative swine serum to be used as internal reference material for quality control.
Actinobacillus pleuropneumoniae ID Screen® APP serotyping kits Comprehensive range of indirect ELISAs for the detection of serotype-specific APP antibodies in swine serum, plasma and meat juice.
Name
Product code
ID Screen APP 1-9-11 Indirect
APPS1-9-11-2P
ID Screen APP 2 Indirect
APPS2-2P
ID Screen APP 2-6 Indirect
APPS2-6-2P
ID Screen APP 2-6-12 Indirect
APPS2-6-12-2P
ID Screen APP 3-6-8 Indirect
APPS3-6-8-2P
ID Screen APP 4-7 Indirect
APPS4-7-2P
ID Screen APP 5 Indirect
APPS5-2P
ID Screen® APP 8 Indirect
APPS8-2P
ID Screen® APP 10 Indirect
APPS10-2P
ID Screen® APP 12 Indirect
APPS12-2P
® ® ® ® ® ® ®
High serotype-specificity and sensitivity Easy-to-use, as there is no sample pre-dilution step, and results are obtained in just 75 minutes The same fast and convenient protocol is used for the entire range of ID Screen® APP ELISAs, making it easier to use several tests at once
Specifications Method
Indirect ELISA
Species
Swine
Specimens
Serum, plasma or meat juice
Coated antigen
APP serotype specific antigens
Conjugate
Anti-swine IgG – HRP conjugate (concentrated 10X)
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.
Associated Products APP positive freeze-dried sera Product Code
MRI-APP-1-9-11
MRI-APP-2
MRI-APP-3-8
MRI-APP-4-7
MRI-APP-5
MRI-APP-6
MRI-APP-10
MRI-APP-12
Format
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freezedried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
APP negative freeze-dried serum Product Code
MRINEG-APP
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried negative swine serum to be used as internal reference material for quality control.
77
SWINE Aujeszky’s Disease / Pseudorabies ID Screen® Aujeszky gB Competition Competitive ELISA for the detection of anti-gB antibodies. Detects antibodies against all Aujeszky’s Disease Virus (ADV) strains further to natural infection or vaccination.
High sensitivity and specificity Meets OIE analytical sensitivity requirements1: calibrated against the OIE ADV-1 reference serum Convenient and flexible : short (results within 2 hours) or overnight protocols Automatable for high throughput testing Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Specifications Method
Competitive ELISA
Species
Swine (domestic pigs and wild boar)
Specimens
Serum or plasma
Coated antigen
Antigenic suspension of the Aujeszky Disease Virus (ADV)
Conjugate
Anti-gB-HRP conjugate (concentrated 10X)
Product code
AUJESZKYGB-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.
Associated Products ADV positive freeze-dried serum (vaccinated animal)
78
Product Code
MRI-AUJGB
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive porcine serum from a vaccinated animal. To be used as internal reference material for quality control. This serum gives a negative result on the ID Screen® Aujeszky gE Competition ELISA.
Aujeszky’s Disease / Pseudorabies ID Screen® Aujeszky gE Competition Competitive ELISA for the detection of anti-gE antibodies in swine serum and plasma. Detects naturally-infected animals in a vaccinated population, provided that animals are vaccinated with a vaccine that does not express the gE protein. For use in ADV vaccination-eradication programs in combination with gEdeleted vaccines.
Sensitive and specific detection of anti-gE antibodies Meets OIE analytical sensitivity requirements1: calibrated against the OIE International Standard Reference Serum May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals) Convenient and flexible protocol : ready-to-use conjugate, with short and overnight protocols Automatable for high throughput testing
Specifications Method
Competitive ELISA
Species
Swine (domestic pigs and wild boar)
Specimens
Serum or plasma
Coated antigen
Antigenic suspension of the Aujeszky Disease Virus (ADV)
Conjugate
Ready to use anti-gE-HRP conjugate
Product code
AUJESZKYGE-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.
Associated Products ADV positive freeze-dried serum (naturally-infected animal) Product Code
MRI-AUJGE
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive porcine serum from a naturally-infected animal. To be used as internal reference material for quality control. This serum is also positive on the ID Screen® Aujeszky gB Competition ELISA.
79
SWINE African Swine Fever ID Screen® African Swine Fever Indirect Multi-antigen indirect ELISA kit for the detection of antibodies against P32, P62 and P72 of the ASFV in porcine serum, plasma, meat juice or blood filter paper samples.
The only commercial ELISA based on 3 recombinant antigens : P32, P62, and P72 High sensitivity and specificity, with clear separation of positive and negative results Convenient and versatile, with ready-to-use components and protocols for serum, plasma, meat juice and blood filter paper samples. Blood filter paper samples may be tested in 96-well deep-well plates, making sample processing faster and less prone to mix-ups
Specifications Method
Indirect ELISA
Species
Swine (domestic pigs and wild boar)
Specimens
Serum, plasma, meat juice or blood filter paper samples
Coated antigen
Mix of P32, P62 and P72 recombinant proteins
Conjugate
Anti-multi-species-HRP concentrated conjugate (10X)
Ordering information Screening format
Confirmation format*
Product code
ASFS-2P
ASFS-4P
Product code
ASFB-4P
Kit format
2 plates
4 plates
Kit format
4 plates (bi-well)
Reactions
192
384
Reactions
192
Plate format
12 x 8-well strips
Plate format
6 x 16 well strips
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
Associated Products ASF positive freeze-dried serum
80
Product Code
MRI-ASF
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive porcine serum to be used as internal reference material for quality control.
Brucellosis
ID Screen® Brucellosis Serum Indirect Multi-species Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).
Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum. Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples.
Specifications Method
Indirect ELISA
Species
Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.
Specimens
Individual serum or plasma samples, or pools of up to 10 samples.
Coated antigen
Purified Brucella LPS
Conjugate
Anti-multi-species-IgG-HRP conjugate (concentrated 10X)
Product code
BRUS-MS-4P
BRUS-MS-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
References (1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis. (2) Annex C of the European directive CEE 64/432
81
SWINE Classical Swine Fever / Hog Cholera ID Screen® Classical Swine Fever E2 Competition Competitive ELISA kit for the detection of anti-E2 antibodies in swine serum or plasma.
Sensitive and specific detection of anti-E2 antibodies Fast and convenient protocol : ready-to-use conjugate, and results in 90 minutes.
Specifications Method
Competitive ELISA
Species
Swine (domestic pigs and wild boar)
Specimens
Serum or plasma
Coated antigen
Recombinant E2 glycoprotein
Conjugate
Ready-to-use anti-E2-HRP conjugate
Product code
CSFE2C-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
Associated Products Classical Swine Fever positive freeze-dried serum
82
Product Code
MRI-CSFE2
Format
1 ml vial (freeze-dried)
Description
Freeze-dried highly positive swine serum to be used as internal reference material for quality control.
Hepatitis E ID Screen® Hepatitis E Indirect Multi-species Indirect ELISA for the detection of anti-Hepatitis E antibodies in serum and plasma from swine and other species. The use of genotype 3 antigens makes the test ideally suited for use in swine, as genotype 3 is the genotype which infects pigs (HEV ELISAs for humans use genotype 1 antigens).
High sensitivity and specificity in swine, thanks to the use of genotype 3 antigens Applicable to multiple species, and validated on swine. Please contact IDvet for more information Straightforward and rapid, with results in 90 minutes
Specifications Method
Indirect ELISA
Species
Swine and other species. Please contact IDvet for more information.
Specimens
Serum or plasma
Coated antigen
Recombinant genotype 3 capsid
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code
HEVB-MS-4P
Kit format
4 plates
Reactions
192 (Biwell format*)
Plate format
6 x 16-well strips
Ordering information
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
References Eiden M., Schlosser M., Groschup J. (Friedrich-Loeffler-Institut, Insel Riems, Germany), Pourquier P., Comtet L. (IDvet). Preliminary validation data of a commercial multi-species ELISA kit for detection of anti-Hepatitis E Virus antibodies in swine. Poster presented at the 6th International Symposium on Emerging and Reemerging Pig Diseases, June 2011, Madrid, Spain.
Associated Products HEV positive freeze-dried serum Product Code
MRI-HEV
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
83
SWINE Influenza A ID Screen® Influenza A Antibody Competition Multi-species Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
Highly sensitive and specific Convenient multi-species test applicable to birds, horses, swine and other species - please contact IDvet for more information Cost-effective for the analysis of large sample numbers, particularly in comparison with Haemagglutination Inhibition (HI) Easy-to-use, with results in less than 2 hours
Specifications Method
Competitive ELISA
Species
Multiple species, including swine, birds, horses and others
Specimens
Serum and plasma
Coated antigen
Influenza A nucleoprotein (NP)
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
FLUACA-2P
FLUACA-4P
FLUACA-10P
Kit format
2 plates
4 plates
10 plates
Reactions
192
384
960
Plate format
12 x 8-well strips
Ordering information
References Swine : (1) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (2) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123. Horses: (3) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014. Birds: (4) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24. (5) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland. (6) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (7) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy. Dogs: (8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
84
Influenza A ID Screen® Influenza A Antigen Capture Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
High sensitivity and specificity Cost-effective for the analysis of large sample numbers Applicable to multiple species, including birds, horses and swine - please contact IDvet for more information Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests Easy-to-use and rapid, with results in less than 1 hour
Specifications Method
Sandwich ELISA
Species
Multiple species, including swine, birds, horses and others.
Specimens
-Broncho-pulmonary liquids from pigs, horses and birds. -Bird cloacae swab samples. -Bird faeces.
Coated antibody
Anti-nucleoprotein (NP) monoclonal antibody
Conjugate
Anti-NP-HRP conjugate (concentrated 10X)
Product code
INFLAG-2P
Kit format
2 plates
Reactions
192
Plate format
96 individual wells
Ordering information
85
SWINE Mycobacterium avium ID Screen® Mycobacterium avium Indirect Multi-species Indirect multi-species ELISA for the detection of antiMycobacterium avium complex (MAC) antibodies in sera and plasma from swine.
Excellent specificity This test is designed to detect circulation of M. avium subsp. hominissuis on the herd level (low test sensitivity for individuals) Cost-effective for high throughput testing, notably in comparison to the incision of lymph nodes at slaughter Convenient protocol, with ready-to-use components and results in 2 hours.
Specifications Method
Indirect ELISA
Species
Swine Please contact IDvet for use in other species
Specimens
Serum, plasma or meat juice
Coated antigen
Purified MAC extract
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code
MA-MS-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References (1) Pourquier, P., Lesceu S. Preliminary validation of the ID Screen® Mycobacterium avium Multi-species Indirect ELISA. Poster presented at the AHVLA International Conference 2011 - Animal Diseases and their Consequences, Royal Holloway, England. (2) Shade B et al. Diagnostic investigations on mycobacterial abortion in a sow herd. Poster presented at the 21st IPVS Congress, Vancouver, Canada, July, 2010.
Associated Products Mycobacterium avium positive freeze-dried serum
86
Product Code
MRI-MA
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
Mycoplasma hyopneumoniae / Enzootic pneumoniae / Mycoplasmal pneumonia of swine ID Screen® Mycoplasma hyopneumoniae Indirect Indirect ELISA kit for the detection of anti-P46 antibodies in swine serum and plasma.
Accurate and reliable, with high specificity and sensitivity Easy-to-use : results in 90 minutes
Specifications Method
Indirect ELISA
Species
Swine
Specimens
Serum or plasma
Coated antigen
Recombinant P46 of M. hyopneumoniae
Conjugate
Anti-swine IgG-HRP conjugate (concentrated 10X)
Product code
MHYOPS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
Associated Products Mycoplasma hyopneumoniae positive freeze-dried serum Product Code
MRI-MHYOP
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
87
SWINE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (prrs) ID Screen® PRRS Competition Competitive ELISA for the detection of PRRSV anti-nucleoprotein (ORF7) antibodies in swine serum samples. Detects antibodies against both the US and European strains.
Detection of both US and European strains
Excellent specificity and sensitivity Convenient protocol, with ready-to-use components and results in 2 hours
Specifications Method
Competitive ELISA
Species
Swine
Specimens
Serum
Coated antigen
Purified PRRSV antigen
Conjugate
Anti-nucleoprotein-HRP conjugate (concentrated 10X)
Product code
PRRS-4P
PRRS-10P
Kit format
4 plates
10 plates
Reactions
384
960
Plate format
12 x 8-well strips
Ordering information
88
Swine Vesicular Disease ID Screen® Swine Vesicular Disease Competition 5B7 Mab Competitive ELISA1,2 for the detection of antibodies directed against the SVD virus in porcine serum or plasma.
High sensitivity and specificity, with clear separation of positive and negative results Calibrated to detect the Primary Reference Serum EU-RS4, provided by the OIE Reference Laboratory, Pirbright, UK2 Convenient and rapid test, with ready-to-use components and results within 2 hours.
Specifications Method
Competitive ELISA
Species
Swine
Specimens
Serum or plasma
Coated antigen
SVDV purified antigen
Conjugate
Mab 5B7-HRP conjugate (concentrated 10X)
Product code
SVDC-4P
Kit format
4 plates
Reactions
384
Plate format
12 x 8-well strips
Ordering information
References (1) Brocchi, E., Berlinzani, A., Gamba, D. & Simone, F. (1995). Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. J. Virol.Methods, 52, 155-67. (2) OIE Terrestrial Manual 2013; 2.8.9; Swine Vesicular Disease.
Associated Products SVD positive freeze-dried serum Product Code
MRI-SVD
Format
0.5 ml vial (freeze-dried)
Description
Freeze-dried positive swine serum to be used as internal reference material for quality control.
89
SWINE Toxoplasmosis ID Screen® Toxoplasmosis Indirect Multi-species Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, dogs and cats.
Easy-to-use, with results in 90 minutes Versatile protocol for serum, plasma or meat juice Applicable to multiple species, including ruminants, swine, dogs and cats
Specifications Method
Indirect ELISA
Species
Multiple species, including ruminants, swine, dogs, cats and others
Specimens
Serum, plasma or meat juice
Coated antigen
Toxoplasma gondii P30 antigen
Conjugate
Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code
TOXOS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
References Swine (1) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara. (2) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009. (3) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011. Sheep (4) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008 (5) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009. Deer (6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010. (7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009. Dogs and cats (8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009. Wild & domestic animals (9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.
Associated Products Toxoplasmosis positive freeze-dried serum
90
Product Code
MRI-TOXO
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive porcine serum (wild boar) to be used as internal reference material for quality control.
Trichinellosis ID Screen® Trichinella Indirect Multi-species Indirect multi-species ELISA kit for the detection of antiTrichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use with other species.
Highly sensitive and specific Applicable to multiple species, including pigs, wild boars and horses Serum, plasma and meat juice protocols
Specifications Method
Indirect ELISA
Species
Multiple species, including pigs , wild boar, horses and others
Specimens
Serum, plasma or meat juice
Coated antigen
Trichinella E/S antigen
Conjugate
Anti-multi-species-HRP conjugate (concentrated 10X)
Product code
TRICHIS-MS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
Associated Products Trichinellosis positive freeze-dried serum
Trichinellosis negative freeze-dried serum
Product Code
MRI-TRICHI
Product Code
MRINEG-TRICHI
Format
1 ml vial (freeze-dried)
Format
1 ml vial (freeze-dried)
Description
Freeze-dried positive porcine (wild boar) serum to be used as internal reference material for quality control.
Description
Freeze-dried negative porcine (wild boar) serum to be used as internal reference material for quality control.
91
92
COMPANION ANIMALS
93
COMPANION ANIMALS Canine Leishmaniasis ID Screen® Leishmaniasis Indirect Indirect ELISA kit for the detection of anti-Leishmania infantum antibodies in canine serum and plasma.
Particularly rapid, notably in comparison with indirect immunofluorescence Improved specificity thanks to the use of a L. infantum purified antigen Easy-to-use, with results in 90 minutes
Specifications Method
Indirect ELISA
Species
Dogs
Specimens
Serum and plasma
Coated antigen
L. infantum purified antigen
Conjugate
Anti-canine IgG–HRP conjugate (concentrated 10X)
Product code
LEISHS-2P
Kit format
2 plates
Reactions
192
Plate format
12 x 8-well strips
Ordering information
Leishmania infantum Immunofluorescent Slides Product Code
IFI-LEISH
Format
10 slides, 10 reactions per slide.
Description
Slides for the serodiagnosis of Leishmania by indirect immunofluorescence (IIF) in dog sera.
Associated Products Leishmania infantum positive freeze-dried serum Product Code
MRI-LEISH
Product Code
MRINEG-LEISH
Format
1 ml vial (freeze-dried)
Format
2 ml vial (freeze-dried)
Description
Freeze-dried, highly-positive serum from a naturally-infected dog.
Description
Freeze-dried negative canine serum.
Produces a positive reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test) To be used as internal reference material for quality control.
94
Leishmania infantum negative freeze-dried serum
Produces a negative reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test) To be used as internal reference material for quality control.
All species
95
All species Complement Fixation Test The complement system is a system of serum proteins that react with antigen-antibody complexes. If this reaction occurs on a cell surface, it will result in the formation of trans-membrane pores and therefore destruction of the cell. This characteristic is used in the complement fixation test (CFT) to detect antibodies against infectious animal diseases. The complement fixation test requires complementary reagents that are not included in the kit. (Calcium Magnesium veronal buffer, haemolysin, sheep red blood cells, and specific antigen for CFT).
Guinea pig complement Freeze-dried guinea pig complement to be used in any
Product Code
CPLT-2x5ML
complement fixation test (CFT)
Format
2 x 5 ml vials
The Veronal Calcium Magnesium buffer is used to dilute
Product Code
VB-4L
sera and reagents for the complement fixation test.
Format
4 vials (powder form). Reconstitute each vial with 1L of distilled water
Once reconstituted, the complement is stable for two weeks at 4°C without loss of activity.
Veronal buffer
Once reconstituted, the veronal buffer is stable for 2 months at 2 – 8°C without loss of activity.
96
DATA ANALYSIS PROGRAM ID Soft™ ID Soft™ is a fully integrated, easy-to-use software program providing convenient, flexible and comprehensive data analysis for ID Screen® ELISA kits.
Features Fully integrated disease management system
Determine titres, vaccination age and baseline values for better preventative medicine for poultry.
Easily share your results with others
OD results may be exported to other formats, such as Excel.
Optimize lab time and plan your testing schedule
The software provides you with an overview of the different tests to be run for each sample.
Flexible
Collect data and edit reports from multiple work stations. Customize your templates and reports, and incorporate your own company information and logo.
Easy access
IDSoft™ may be downloaded from the IDvet website – no discs are required.
Installing ID Soft™ 1) Download ID Soft™ from the IDvet website: www.id-vet.com/support/software. 2) Contact
[email protected] to obtain the User Guide and your personal login and password. 3) Use your login and password to download kits to your repertory or to install updates as they become available.
Reader compatibility TECAN
Sunrise
MOLECULAR DEVICES
EMax EL311 EL800 EL808
BIOTEK
ELx800 ELx808
Minimum system requirements Microsoft Windows 2000, XP, Vista, Windows 7, Windows 8 50 MB of free hard-disk space required Internet Connection
97
All species Positive and negative freeze-dried sera and milk to be used as internal reference material for quality control*
Disease
Status
Type
Species
Volume
Product code
For use with**
RUMINANTS AKABANE
Positive
Serum
Sheep
1 ml
MRI-AKA
AKAC
BESNOITIOSIS
Positive
Serum
Cattle
1 ml
MRI-BSNT
BSNTS
BHV-4
Positive
Serum
Catlle
0.5 ml
MRI-BHV4
BHV4S
BLUETONGUE
Positive
Serum
Cattle
1 ml
MRI-BTC
BTC, BTED
Positive
Milk
Cattle
1 ml
MRI-BTMILK
BTSMILK
BLV
Positive
Serum
Cattle
1 ml
MRI-BLV
BLVC, BLVS
BRUCELLOSIS
Positive
Serum
Cattle
1 ml
MRI-BRU
BRUS-MS, Rose Bengal, CFT, SAW
Negative
Serum
Cattle
1 ml
MRINEG-BRU
BRUS-MS, Rose Bengal, CFT
Positive
Milk
Cattle
BVD/BD/MD
Positive
Serum
Cattle
Positive
Milk
Cattle
CHLAMYDOPHILA ABORTUS
Positive
Serum
IBR
Positive
COMING SOON 1 ml
MRI-BVD
BVDOS, BVDC
Sheep
1 ml
MRI-CHLM
CHLMS-MS
Serum
Cattle
1 ml
MRI-IBR
IBRS, IBRGBC, IBRGEC
Positive
Milk
Cattle
INTERFERON GAMMA
Positive
Plasma
Cattle
0.5 ml
MRI-IFNG-B
IFNG
(NATIVE)
Positive
Plasma
Sheep
0.5 ml
MRI-IFNG-O
IFNG
Positive
Plasma
Goat
0.5 ml
MRI-IFNG-C
IFNG
COMING SOON
COMING SOON
Positive
Plasma
Buffalo
MVV / CAEV
Positive
Serum
Sheep and Goat
0.5 ml
MRI-VISNA/CAEV
VISNAS/VISNAB
NEOSPORA CANINUM
Positive
Serum
Cattle
0.5 ml
MRI-NC
NCS-MS
PARATUBERCULOSIS
Positive
Serum
Cattle
1 ml
MRI-PARA
PARAS, PARAB
Negative
Serum
Cattle
2 ml
MRINEG-PARA
PARAS, PARAB
Positive
Serum
Goat
1 ml
MRI-PPR
PPRC
Positive
Antigen
-
1 ml
MRI-PPRAG
PPRAG
Q FEVER
Positive
Serum
Cattle
1 ml
MRI-FQ
FQS-MS
RIFT VALLEY FEVER
IgG-positive
Serum
Cattle
1 ml
MRI-RIFT
RIFTC
IgM-positive
Serum
Cattle
0.5 ml
MRI-RIFTIGM
RIFTIGM
SCHMALLENBERG VIRUS
Positive
Serum
Cattle
0.5 ml
MRI-SBV
SBVC, SBVS, SBVB
NEGATIVE BOVINE SERUM
Negative
Serum
Cattle
0.5 ml
MRINEG-BOV
ID Screen® kits for Aka, BLV, Bru, BTV, IBR, Paratb, NC, SBV
Positive
Serum
Chicken
0.5 ml
MRI-IBDS
IBDS
Positive
Serum
Chicken
0.5 ml
MRI-IBDVP2
IBDVP2
IBV
Positive
Serum
Chicken
0.25 ml
MRI-IBV
IBVS, IBVC
ILT
Positive
Serum
Chicken
0.5 ml
MRI-ILT
ILTS
INFLUENZA A
Positive
Serum
Chicken
1 ml
MRI-FLUACA/N1
FLUACA, FLUACN1
Positive
Serum
Chicken
0.5 ml
MRI-FLUACH7
FLUACH7
PPR
COMING SOON
AVIAN IBD
* Contain 0.03% Merthiolate as a preservative ** IDvet product code or technique
98
Disease MYCOPLASMA
Status
Type
Species
Volume
Product code
For use with*
Positive
Serum
Chicken
0.5 ml
MRI-MG
MG/MS, MG, RSA-MG
Positive
Serum
Turkey
1 ml
MRI-MM
RSA-MM
MYCOPLASMA SYNOVIAE
Positive
Serum
Chicken
1 ml
MRI-MS
MG/MS, MS, RSA-MS
NDV
Positive
Serum
Chicken
0.5 ml
MRI-NDV
NDVC, NDVS
SALMONELLA B & D GROUPS
Positive
Serum
Chicken
0.5 ml
MRI-SALS
SALS
NEGATIVE SPF CHICKEN
Negative
Serum
Chicken
0.5 ml
MRINEG-BIRD
ID Screen® ELISAs for avian
GALLISEPTICUM MYCOPLASMA MELEAGRIDIS
diseases
SERUM
EQUINE EIA
Positive
Serum
Horse
1 ml
MRI-EIA
EIAED
EVA
Positive
Serum
Horse
0.5 ml
MRI-EVA
EVAB
STRANGLES
Positive
Serum
Horse
0.5 ml
MRI-STREPTO
STREPTOS
WEST NILE
IgG-positive
Serum
Horse
1 ml
MRI-WN
WNC
IgM-positive
Serum
Horse
0.5 ml
MRI-WNIGM
WNIGM
AFRICAN SWINE FEVER
Positive
Serum
Pig
0.5 ml
MRI-ASF
ASFB
APP
Negative
Serum
Pig
0.5 ml
MRINEG-APP
ID Screen® APP ELISAs
Positive
Serum
Pig
0.25 ml
MRI-APP-2
APPS2
Positive
Serum
Pig
0.25 ml
MRI-APP-1-9-11
APPS1-9-11
Positive
Serum
Pig
0.25 ml
MRI-APP-4-7
APPS4-7
Positive
Serum
Pig
1 ml
MRI-APP-3-8
APPS3-8
Positive
Serum
Pig
0.25 ml
MRI-APP-5
APPS5
Positive
Serum
Pig
1 ml
MRI-APP-6
APPS6
Positive
Serum
Pig
0.25 ml
MRI-APP-10
APPS10
Positive
Serum
Pig
0.25 ml
MRI-APP-12
APPS12
Positive
Serum
Pig (vaccinated)
1 ml
MRI-AUJGB
AUJESZKYGB
Positive
Serum
Pig (infected)
1 ml
MRI-AUJGE
AUJESZKYGE
CSF
Positive
Serum
Pig
1 ml
MRI-CSFE2
CSFE2C
HEV
Positive
Serum
Pig
1 ml
MRI-HEV
HEVB-MS
MYCOBACTERIUM AVIUM
Positive
Serum
Pig
1 ml
MRI-MA
MA-MS
MYCOPLASMA
Positive
Serum
Pig
1 ml
MRI-MHYOP
MHYOPS
SVD
Positive
Serum
Pig
0.5 ml
MRI-SVD
SVDC
TOXOPLASMOSIS***
Positive
Serum
Pig
1 ml
MRI-TOXO
TOXOS-MS
TRICHINELLOSIS
Positive
Serum
Pig
1 ml
MRI-TRICHI
TRICHIS-MS
Negative
Serum
Pig
1 ml
MRINEG-TRICHI
TRICHIS-MS
Positive
Serum
Dog
1 ml
MRI-LEISH
LEISHS, IFI-LEISH
Negative
Serum
Dog
2 ml
MRINEG-LEISH
LEISHS, IFI-LEISH
PORCINE
AUJESZKY’S DISEASE
HYOPNEUMONIAE
COMPANION ANIMALS CANINE LEISHMANIASIS
*** Note: The negative APP serum (MRINEG-APP) is also negative for Toxoplasmosis.
99
Phone + 33 (0) 4 67 41 49 33 Fax + 33 (0) 4 67 45 36 95
[email protected] www.id-vet.com
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