Neuron, Volume 90

Supplemental Information

Linking Cholinergic Interneurons, Synaptic Plasticity, and Behavior during the Extinction of a Cocaine-Context Association Junuk Lee, Joel Finkelstein, Jung Yoon Choi, and Ilana B. Witten

Supplemental Figures and Legends

Supplementary Figure 1: Optogenetic activation of cholinergic interneurons enhances the extinction of a cocaine conditioned place preference but has no effect on measures of anxiety. A. Cre-dependent ChR2-YFP (or YFP-only) virus was injected bilaterally into the NAc of ChAT::IRES-Cre mice with a 129S1 background and fibers were

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implanted above the injection sites. B. Cocaine CPP paradigm with optogenetic stimulation on “Test 1”. Cocaine chamber preference is measured during the baseline test before conditioning and the three test days following conditioning for mice undergoing a cocaine CPP (paradigm identical to Figure 1E) (F(2,26)=10.10 p=0.02 for group, repeated measures ANOVA. ChR2 group, (n=8) Pre-test: -11.505 ± 42.1, Test 1: 274.9 ± 58.6, Test 2: 92.3 ± 47.8, Test 3: 122.2, ± 106.2. YFP group, (n=7) Pre-test: -34.3 ± 28.2, Test 1: 418.8 ± 50.4, Test 2: 236.4 ± 42.4, Test 3: 245.0 ± 102.4). C. Time course of chamber preference for the first extinction test from all experimental animals undergoing cocaine CPP that received optogenetic activation of ChAT interneurons (along with corresponding control cohorts). (ChR2 group, (n=46) Control group, (n=45); p=0.001 for time, p