Sunday, May 29th 14.30- 16.30
16.00 - 16.30
REGISTRATION Welcome (Prof. Alessandro Ballio, Prof. Giorgio Federici, Prof. Franco De Angelis Presidente della Società di Chimica Italiana, Giancarlo Gabianelli Sindaco della città di Viterbo, Alessando Mazzoli Presidente della Provincia di Viterbo, Prof. Vincenzo Buonocore Preside della facoltà di Scienze, Prof. Massimo Mazzini Dir. Dipartimento di Scienze Ambientali)
Opening lectures Chair:
Pier Giorgio Righetti
16.30 - 17:15
PROTEOMICS WHY, HOW AND WHEN P. Roepstorff Department of Biochemistry and Molecular Biology University of Southern Denmark, DK 5230 Odense M, Denmark
17.15 - 18.00
PEPTIDE CENTRIC PROTEOMICS: A CHALLENGE FOR PROTEIN CHEMISTRY, CHROMATOGRAPHY, ROBOTICS, MASS SPECTROMETRY AND BIOINFORMATICS J. Vandekerckhove and K. Gevaert Department of Biochemistry, VIB09, Ghent University, Belgium
18.00 - 18.45
FROM GENOMICS TO BREEDING IN PLANTS M. Morgante Dipartimento di Scienze Agrarie e Ambientali, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy.
19.00
Welcome Cocktail
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Monday, May 30th 9:00 - 9:30
Welcome (Magnifico Rettore Prof. Marco Mancini, Prof. Giuseppe Rotilio Presidente della Società Italiana di Biochimica, Dr. Arch. Cocullo Direttore Amministrativo Università “La Tuscia”
Plant Proteomics Chair
Giuseppe Rotilio, Felice Cervone
9:30 - 10:10
EXPLORING THE PLASTID PROTEOME FOR PATHWAY PREDICTION AND FUNCTION. W. Gruissem, T. Kleffmann, A. von Zychlinski, A. Siddique, D. Russenberger, M. Hirsch-Hoffmann and S. Baginsky. Institute of Plant Sciences and Functional Genomics Center Zurich, Swiss Federal Institute of Technology, (ETH Zürich), 8092 Zürich, Switzerland.
10:10 - 10:40
BREAKING THE TABOO OF GENOTYPING ERRORS. A. Bonin1 , E. Bellemain1,2 , P. Bronken Eidesen3 , F. Pompanon 1 , C. Brochmann3, P. Taberlet1 1 Laboratoire d'Ecologie Alpine, CNRS-UMR 5553, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 09, France. 2 Department of Ecology and Natural Resource Management, Agricultural University of Norway, Box 5003, NO-1432 Ås, Norway. 3 National Centre for Biosystematics, Natural History Museums and Botanical Garden, University of Oslo, PO Box 1172 Blindern, NO-0318 Oslo, Norway.
10:40 - 11:10
THE INTERNATIONAL SOLANACEAE GENOME PROJECT: SYSTEMS APPROACH TO PLANT BIODIVERSITY. G. Giuliano ENEA, Casaccia, Roma
11:10 - 11:40
coffee break
Human Proteomics I Chair:
Giorgio Federici, Paolo Moccarelli
11:40 - 12:20
REDOX PROTEOMICS IN ALZHEIMER’S DISEASE AND MODELS THEREOF: ROLE OF AMYLOID BETA-PEPTIDE AND INSIGHT INTO MECHANISMS OF NEURODEGENERATION. A. Butterfield Department of Chemistry, Center of Membrane Sciences, and SandersBrown Center on Aging, University of Kentucky, Lexington, KY 40506 USA
12:20 - 12:35
DIFFERENTIAL POST-TRANSLATIONAL MODIFICATIONS OF TRANSTHYRETIN IN ALZHEIMER DISEASE: A STUDY IN THE CEREBRAL SPINAL FLUID. A. Biroccio4 , P. Del Boccio1,2, C. Aceti3 , S. Bernardini3 , C. Di Ilio1,2 , D. Gambi1, P. Stanzione5, P. Sacchetta1,2, G. Bernardi5,6 A. Martorana5,6, G. Federici3,4, A. Stefani5 and A. Urbani1,2 1 Centro Studi sull’Invecchiamento (Ce.S.I.), Chieti, Italy. 2Dipartimento di Scienze Biomediche, Università “G. D’Annunzio” di Chieti e Pescara, Italy. 3 Dipartimento di Medicina di Laboratorio, Policlinico di Tor Vergata, 2
Università di Roma Tor Vergata, Roma. 4Laboratori di Ricerca, Ospedale Pediatrico del Bambino Gesù – IRCCS, Roma, Citta’ del Vaticano. 5IRCCSFondazione S. Lucia, Roma. 6Clinica Neurologica, Università di Roma Tor Vergata, Roma 12:35 - 12:50
PROTEOMIC ANALYSIS OF NEUROBLASTOMA TISSUES N. Campostrini1, F. Pastorino2, D. Marimpietri2, H. Astner3, M. Hamdan3, L. B. Areces4, J. Rappsilber4, M. Ponzoni2, P.G. Righetti1 1 University of Verona, Department of Agricultural and Industrial Biotechnologies, Verona, Italy; 2 Laboratory of Oncology, G. Gaslini Children’s Hospital, Genoa, Italy; 3Computational, Analytical and Structural Sciences, GlaxoSmithKline, Verona, Italy; 4 FIRC Institute for Molecular Oncology Foundation (IFOM) and European Institute of Oncology (IEO), Milano, Italy.
12:50-13:05
NON ENZYMATIC PROTEIN GLYCATION AND ITS RELEVANCE IN DIABETES A. Lapolla1 and P.Traldi2 1 Dipartimento di Scienze Mediche e Chirurgiche, Cattedra di Malattie del Metabolismo, Università di Padova, Via Giustiniani 2, I-35100 Padova, Italy. 2 CNR-ISTM, Corso Stati Uniti 4, I-35100, Padova, Italy.
13:10 - 15:00
Lunch break, industrial exhibition
13:10 - 15:00
POSTER SESSION I
Genomics Chair:
Alessio Valentini, Giovanni Candiano
15:00 - 15:40
PHYSICAL MAPS AND WHOLE GENOME SEQUENCES: THE IDEAL TOOLS FOR GENE HUNTING IN DOMESTIC ANIMALS? Duchesne A. & Eggen A. LGbC – INRA, Department of Animal Genetics, Jouy-en-Josas, France
15:40 - 16:10
SELECTION SIGNATURES DISCOVERED BY A POPULATION GENOMIC APPROACH. M.A. Beaumont Reading, UK
16:10 - 16:40
MALDI MASS SPECTROMETRY OF NUCLEIC ACIDS AND THE IMPLICATIONS FOR SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS. J. Mengel-Jorgensen1, J. Sanches1, N. Morling1, C. Borsting1 and F. Kirpekar2 1 Department of Forensic Genetics, University of Copenhagen, Denmark. 2 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark.
16:40 - 17:10
coffee break
Genomics Chair: 17:10 - 17:40
Andrea Urbani, VACCINE DISCOVERY AND PROTEOMICS. G. Grandi Chiron Vaccines, Siena 3
Human Proteomics II 17:40 - 17.55
THE EXPRESSION OF HS1, A SUBSTRATE OF B-CELL-RECEPTOR STIMULATION, DIFFERS IN CHRONIC LYMPHOCYTIC LEUKEMIA SUBSETS C. Scielzo1 , P. Ghia1 , A. Conti2 , A. Bachi2 , G. Guida3 , M. Geuna3 , F. Caligaris-Cappio1 and M. Alessio2 1 Università Vita Salute - San Raffaele, Milan, 2San Raffaele Scientific Institute, Milan, 3IRCC, Candiolo (TO), Italy.
17:55 - 18:10
IDENTIFICATION OF TUMOR ASSOCIATED ANTIGENS IN PANCREATIC CANCER BY SEROLOGICAL PROTEOME ANALYSIS (SERPA) B. Tomaino1, P. Cappello1 , M. Capello1 , C. Fredolini1 , C. Medana2 , M. Alessio3, A. Ponzetto 1, M. Giovarelli1 and F. Novelli1 1 Center for Experimental Research and Medicinal Studies, CeRMS, Dep. of Internal Medicine, S. Giovanni Battista Hospital, Turin 2 Dep. of Analitical Chemistry, University of Turin 3 Dibit S. Raffaele Institute, Milan.
18:10 - 18:25
IDENTIFICATION OF PLASMA MEMBRANE PHOSPHOPROTEINS FROM HUMAN STEM CELLS USING TiO2 PURIFICATION AND MASS SPECTROMETRY. T. E. Thingholm1, M.R. Larsen1, L.J. Foster1, M. Kassem2, O.N. Jensen1 1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark. 2Clinic of Endocrinology Treatment, Odense University Hospital, Odense, Demnark
18:25 - 18:40
NEW PROTEOMICS AND QUANTITATIVE MRI-BASED METHODS FOR BIO-MOLECULAR MARKERS DISCOVERY IN MULTIPLE SCLEROSIS. R. De Masi1,2,3, M. Greco1, G. Indino1, C. Barba5, E. Blasi5, G. Aloisio5, S. Pasca3, G. Trianni3, L. Spagnolo4, S. Armenise4, G. Morciano4, B. Salvato2, M. Maffia1 1 Department of Biological and Environmental Science, University of Lecce Italy; 2 Department of Biology University of Padua - Italy; 3 Division of Neurology, “F. Ferrari” Casarano Hospital AUSL LE/2- Italy; 4 Dision of Neuroradioloy, “F. Ferrari” Casarano Hospital AUSL LE/2- Italy, 5Center for Advanced Computational Technologies Department of Innovation Engineering, University of Lecce - Italy
21:00
Social Dinner
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Tuesday, May 31th Methodologies and bioinformatics Chair:
Gabriella Pocsfalvi, Massimo Alessio
9:00 - 9:15
LIPOSOME CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS. D. Corradini, G. Mancini, C. Bello Institute of Chemical Methodologies, CNR, Area della Ricerca di Roma 1, Montelibretti, P.O. Box 10, 00016 Monterotondo Stazione (Rome) Italy.
9:15 - 9:30
CHIP-BASED BIOAFFINITY ASSAYS: NEW TOOL FOR PROTEIN ANALYSIS. Z. Bílková1, N. Minc2, M. Slováková1,2, L. Korecká1, J.-L. Viovy2 1 Department of Biological & Biochemical Sciences, University of Pardubice, Nam. Cs. Legii 565, 532 16 Pardubice, Czech Republic 2 Laboratory of Physical Chemistry, Institute Curie (UMR CNRS/IC 168), Paris, Cedex 05, France
9:30 - 10:00
HIGHLY SELECTIVE ENRICHMENT OF PHOSPHORYLATED PEPTIDES FROM COMPLEX MIXTURES USING TITANIUM DIOXIDE MICROCOLUMNS. Larsen, M.R. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
10:00 - 10:15
USE OF FTICR MS IN PROTEOMICS. J. Rappsilber FIRC, Institute for Molecular Oncology (IFOM) in Milan, Italy
10:15 - 10:30
MULTIVARIATE STATISTICAL TOOLS FOR THE STUDY AND CLASSIFICATION OF PROTEOMIC 2D-PAGE MAPS: APPLICATION TO HUMAN LYMPHOMAS AND ENDOTHELIA. E. Marengo1 , E. Robotti1 , M.C. Liparota1 , M. Bobba1 , P.G. Righetti2 , F. Antonucci2 , C. Rustichelli2 , N. Campostrini2 , A. Totolo2 , A. Zamò3 , M. Chilosi3, M. Hamdan4, D. Marimpietri4, M. Ponzoni5 1 Department of Environmental and Life Sciences - University of Eastern Piedmont - Via Bellini 25/G - 15100 Alessandria - Italy; 2 Scientific and Technological Department - University of Verona - Strada le Grazie 15 Verona - Italy; 3Department of Pathology - University of Verona - Verona Italy; 4 Department of Computational, Analytical and Structural Science Discovery Research - GlaxoSmithKline S.p.A - Verona - Italy; 5Laboratory of Oncology - G. Gaslini Children’s Hospital - Genoa - Italy
10:30 - 10:50
REDUCING PROTEIN CONCENTRATION RANGE OF BIOLOGICAL SAMPLES USING LIGAND LIBRARIES. E. Boschetti1, P.G. Righetti2, , L. Lomas1, S. Wenberger1, A. Castagna3, V. Thulasiraman1, S. Lin1, L. Guerrier1, F. Fortis1 1 Ciphergen Biosystems Inc., Fremont CA 94555, USA. 2 University of Verona, Dept Agricultural and Industrial Biotechnologies, 37134 Verona, Italy. 3 University of Verona, Dept Clinical and Experimental Medicine, 37134 Verona, Italy.
10:50 - 11:10
TOOLS FOR SAMPLE PREPARATION AND FRACTIONATION IN 2D ELECTROPHORESIS. 5
A. Posch, A. Paulus and M. G. Brubacher Bio-Rad Laboratories, Hercules, CA,USA 11:10 - 11:40
coffee break
Methodologies and bioinformatics Chair:
Andrea Scaloni
11:40 - 11:55
PROTEIN AND RNA ANALYSIS: METHODS FOR LASER CAPTURE MICRODISSECTION TISSUE FRACTION. M. Di Vito, P. Sale Dept. Exp. Med. And Pathol. University “La Sapienza” Rome
11:55 -12:10
PROTEOMICS DECODING 2-D PAGE COMPLEX MAPS: RELEVANCE TO PROTEOMICS. M.C. Pietrogrande1, N. Marchetti1, F. Dondi1 and P.G. Righetti2 1 Department of Chemistry, University of Ferrara, via L. Borsari, 46 I-44100 Ferrara, Italy. 2 University of Verona, Laboratory of Proteome Analysis, Strada Le Grazie 15, Verona 37134, Italy
12:10 - 12:30
A NEW DATA INDEPENDENT LC.(CID)-MS STRATEGY FOR QUANTITATIVE AND QUALITATIVE PROTEIN PROFILING. M.A. McDowall1, S. Geromanos, K. Richardson1, P. Young1, R. Denny1, K. Neeson1, T. McKenna1, C. Doreschel2, M. Gorenstein2, G.-Z Li2, T. Riley2, J.C. Silva2 and J. Langridge1. 1 Waters MS Technologies Centre, Manchester, UK. 2 Waters Corporation, Milford MA, USA.
12:30 - 12:50
USING CHIP-LCMS FOR PROTEOMICS. M. Zanotti Agilent Technologies Italia: Via P. Gobetti 2/C, I-20063 Cernusco s/N MI
12:50 - 13:10
SOME COMMENTS ABOUT THE DE-NOVO SEQUENCING OF PEPTIDES USING MS EQUIPMENT. U. Rapp, M. Macht, D. Suckau, A. Resemann Bruker Daltonik GmbH, Bremen, Germany
13:10 - 15:00
Lunch break, industrial exhibition
13:10 - 15:00
POSTER SESSION II
Genomics Chair:
Luigi Ceci,
15:00 - 15:30
PROTEOMICS APPLIED TO IDENTIFY ENZYME GENES. J. Beekwilder Plant Research International, Postbus 16, 6700 AA Wageningen, The Netherlands.
15:30 - 16:00
GISC AND GENETICS: EXPLOITING THE GEOGRAPHICAL DIMENSION OF MOLECULAR DATA. S. Joost University of Lausanne
16:00 - 16:30
COMBINING CLONE MATERIALS, GENOMES, ANNOTATION AND PREDICTION– FUNCTIONAL GENOMICS IN PRACTICE. 6
S. Hennig, Bernd Drescher, Andreas Hewelt, Johannes Maurer German Resource Center for Genome Research GmbH, Heubnerweg 6, D14059 Berlin, Germany. 16:30 - 17:00
coffee break
Human Proteomics III Chair: 17:00 - 17:15
HUMAN MUSCLE PLASTICITY AND FUNCTION: FROM PHYSIOLOGY TO PATHOLOGY BY DIFFERENTIAL DISPLAY PROTEOMIC. C. Gelfi1, A.Viganò1,2, M. Ripamonti1 , D. Capitanio1 , S. De Palma1 , M. Moriggi, P. Cerretelli2, R. Wait3. 1 IBFM, National Research Council, Segrate (Milan) Italy 2 Department of Sciences and Biomedical Technologies, University of Milano, Italy 3 Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London UK.
17:15 - 17:30
PROTEOMIC ANALYSIS OF PANCREATIC DUCTAL CARCINOMA CELLS AFTER A COMBINED TREATMENT WITH GEMCITABINE AND TRICHOSTATIN-A D. Cecconi1, M. Donadelli3, A. Milli1, A. Scarpa2, M. Palmieri3, M. Hamdan4, L. B. Areces5, J. Rappsilber5 and P.G. Righetti1 1 University of Verona, Department of Agricultural and Industrial Biotechnologies, Verona, Italy 2 University of Verona, Department of Pathology, Section of Anatomical Pathology Verona, Italy 3 University of Verona, Department of Neurological and Visual Sciences, Section of Biochemistry, Verona, Italy 4 Computational, Analytical & Structural Sciences, GlaxoSmithKline, Via Fleming 4, 37135 Verona, Italy 5 FIRC Institute for Molecular Oncology Foundation (IFOM) and European Institute of Oncology (IEO), Via Adamello 16, Milano, Italy
17:30 - 17.45
A PROTEOMIC APPROACH IN THE CHARACTERIZATION OF HUMAN PLASMA PROTEINS IN INDIVIDUALS CARRYING THE MTHFR C677T MUTATION. M. Greco1, F. Chiriacò1 , P. Del Boccio3 , A.C. Gioia1 , L.Tagliaferro2 , P. Menegazzi2, E. Pinca2, F. Pignatelli2, A. Urbani3, C. Storelli1, M. Maffia1 1 Department of Biological and Environmental Sciences and Technologies, University of Lecce, Via Monteroni km7 73100 - Lecce (Italy) 2 “P. Pignatelli” Laboratory, Via Martiri D’Otranto, 73100 - Lecce (Italy). 3 Ce.S.I., Department of Biomedical Sciences, University of Chieti Via Colle dell’Ara, 66100 – Chieti (Italy)
17:45 - 18:00
HIGH RESOLUTION URINE PROTEOMIC PROFILING: 2D COMPARATIVE ANALYSIS AND URINARY PROSTASIN MAPPING IN NORMOTENSIVE INDIVIDUALS AND HYPERTENSIVE PATIENTS WITH PRIMARY ALDOSTERONISM. A. Castagna°*, O. Olivieri*, P. Guarini*, L. Chiecchi*, G. Sabaini°, F. Pizzolo*, R. Corrocher*, P. G. Righetti°. *Department of Clinical and Experimental Medicine, Unit of Internal Medicine,University of Verona, Italy °Department of Science and Technology, University of Verona, Italy.
18:00 - 18:15
PROTEOMIC STUDIES ON LOW- AND HIGH-GRADE 7
HUMAN BRAIN ASTROCYTOMAS. F. Odreman1, M. Vindigni2, M.L. Gonzales1, B. Niccolini1, G. Candiano3, B. Zanotti2, M. Skrap2, S. Pizzolitto4, G. Stanta1, and A. Vindigni1 1 International Centre for Genetic Engineering and Biotechnology, Trieste, Italy; 2 Neurosurgery Unit, Hospital “S.M. della Misericordia” Udine, Italy; 3 Laboratory of Pathophysiology of Uremia, G. Gaslini Children s Hospital, Genova, Italy; 4 Pathology Unit, Hospital “S.M. della Misericordia” Udine, Italy.
18:15
Assemblea dei soci
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Wednesday, June 1st Microorganism Proteomics Chair:
Enrica Pessione
9:00 - 9:40
CANDIDA ALBICANS IN THE SIGHT POINT: PROTEOMICS WEAPONS TO MANAGE THE DIAGNOSIS AND TREATMENT OF SYSTEMIC CANDIDIASIS. A.Pitarch, E. López, R. Martínez, R. Hernández, E. Fernández-Arenas, V. Cabezón, L. Martínez-Solano, R. Faubel, L. Monteoliva, G. Molero, R. Díez-Orejas, C. Nombela and C. Gil. Departamento de Microbiología II, Unidad de Proteómica UCM-Parque Científico de Madrid, Universidad Complutense de Madrid, 28040-Madri.
9:40 - 9:55
A STUDY OF STREPTOCOCCUS THERMOPHILUS PROTEOME BY INTEGRATED ANALYTICAL PROCEDURES AND DIFFERENTIAL EXPRESSION INVESTIGATIONS. C. D’Ambrosio, S. Arena, G. Renzone, R. Rullo, G. Maglione, L. Ferrara, and A. Scaloni Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, 80147 Naples, Italy
9:55 - 10:10
ART-LOVING BUGS: THE RESURRECTION OF SPINELLO ARETINO FROM PISA’S CEMETERY. Antonioli P.1, Zapparoli G.1, Abbruscato P.2 , Sorlini C.2, Ranalli G .3 and Righetti P.G. 1 1 Department of Industrial and Agricultural Biotechnologies, University of Verona, Strada Le Grazie 15, Verona 37134; 2 Department of Food Science and Microbiology, University of Milano, Via Celoria 2, Milano 20133; 3 Department of Food, Agrarian and Environment Science, University of Molise, Campobasso 86100, Italy
10:10 - 10:25
ALKALINE PROTEOME OF LACTOBACILLUS HILGARDII: TOWARDS A COMPREHENSIVE PROTEOME OF LACTOBACILLI C. Lamberti 1 , R. Mazzoli1 , C. Barello2, M.G. Giuffrida2, E. Pessione1, C. Giunta1 1 Dipartimento di Biologia Animale e dell’Uomo, Via Accademia Albertina 13, 10123 Torino,Italy. 2 ISPA-CNR c/o Bioindustry Park Canadese, Via Ribes 5, 10010 Colleretto Giocosa (TO), Italy
Food Chair:
Maria Gabriella Giuffrida
10:25 - 10:40
THE ROLE OF BACTERIAL PROTEIN TOXINS IN FOOD-BORNE DISEASES: CHARACTERIZATION OF VIRULENCE FACTORS OF DIFFERENT STRAINS OF STAPHYLOCOCCUS AUREUS. G. Pocsfalvi Proteomic and Biomolecular Mass Spectrometry Centre, Institute of Food Science and Technology, CNR, Avellino, Italy
10:40 - 11:00
MILK DERIVED BIOACTIVE PEPTIDES: A NOVEL STRATEGY OF PRODUCTION AND PURIFICATION. M. Losacco 1, R. Gallerani 2,F. Pisani 2, F. De Leo3 9
1
Department Of Plant Protection and Applied Microbiology, University of Bari – Italy; 2Department of Biochemistry and Molecular Biology, University of Bari Italy; 3Institute of Biomembranes and Bioenergetics, C.N.R. of Bari Italy 11:00 - 11:15
11:15 - 11:45
PROTEINASE PATTERN CHANGES DURING LARVAL HONEY-BEE DEVELOPMENT Elena Donadio1, Barbara Baldacci1, Maria Gabriella Giuffrida2, Donatella Fortunato2, MauroPinzauti3 , Ettore Balestreri4 , Romano FeIicioIi 1,4 a n d Antonio Felicioll1 1 Dipartimento di Anatomia, Biochimica e Fisiologia Veterinaria, Università di Pisa, Italy. 2 ISPA-CNR, Bioindustry Park del Canavese, Torino, Italy. 3 Dipartimento di Difesa e Coltivazione delle Specie Legnose, Università di Pisa, Italy 4 lstituto di Biofisica, CNR, Italy coffee break
11:45 - 12:00
GLYCOSTRUCTURE INVESTIGATION ON LACTADHERIN USING PROTEOMIC APPROACH. Perono Garoffo L. 1, Fortunato D. 1, Barello C. 1, Baro C. 1 Coscia A .2, Bertino E.2 and Giuffrida M.G. 1 1 ISPA-CNR, Bioindustry Park del Canavese, Torino, Italy.
12:00 - 12:20
POSTER AWARDS
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