ITEM No. 1

Specifications for Bio-safety cabinet Type B-2 (1 No.) 

Should be NSF-49 certified

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Microprocessor controlled Air controlled Class B2 cabinet.

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Less energy consumption and heat output.

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Single piece internal wall.

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Working area: 4 ft width with single piece stainless steel surface.

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Ergonomic design.

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Antimicrobial coating

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Long life ULPA supply filter and HEPA exhaust filter for airflow.

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Exhaust Vent manifold assembly. With structural’s and fittings as appropriate.

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Installation & demonstration at site.

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Warranty of all the parts including the filters for at least 3 years.

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On site service as and when required

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2 nos. of power points 5/15 amp (Indian style) inside the cabinet

Accessories: 

Minimum of 2 HP of exhaust blower

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Full fitting and wiring

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Germicidal UV lamp and fluorescent lamp

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On line UPS (3KVA) with 1 hr backup.

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The system should be supplied with 1.5ton (five star rating) split air conditioning unit with appropriate wall mounted stabilizer (140V – 240V) in order to maintain ambient temperature No.5-70/2016-17/P&S/Ash

ITEM N0.2

Specification for Research microscope with photographic image analysis system attached with PC Binocular upright microscope with infinity corrected optical system, 25mm stage stroke with fine and coarse adjustment knobs, illumination transmitted light with at least 12 volts, 100watt halogen bulb, six positing revolving nose piece with slot for DIC. Widefield 3 ways light distribution eye piece tube, swing out condenser, paired eye piece 10X (F.O.V. 22mm or batter, anti fungus type and with eye guard cap) mechanical stage with capability to hold two slides at the same time. Plan achromatic objectives (2X, 4X, 10X, 20X) and plan fluorite (40X and 100X oil). Dedicated digital cooled CCD camera with minimum resolution of 5.0 Mega Pixel The specification are generic in nature Image Analysis Software: - image analysis software for image acquisition and annotation capable of measuring quantitative data such as intensity, line profile, spatial measurements such as length, width, area, perimeter, number of cells in area with specific morphology etc. Data Acquisition System:- Branded PC with at least i5 processor, 4 GB or better RAM, 500 GB or better HDD, DVD RW, Genuine Windows 10 OS, LED monitor 32 inch, keyboard and optical mouse and UPS Suitable for the whole assembly with back up of minimum 1h should be provided. NOTE:- MICROSCOPE, CAMERA & SOFTWARE SHOULD BE FROM SAME MANUFACTURER FOR BETTER COMPATIBILITY & UPGRADABILITY OPTION Warranty of at least two years should be provided. No.5-57/2016-17/P&S/

ITEM No. 3

Technical specifications of Gradient PCR 1. The system should have 96 well format with at least six separate peltier blocks to provide independent temperature zones to run six different assays with different annealing temperature at the same time. 2. The system should be capable to run up to six separate temperatures in the same plate with user’s defined time. 3. The system should be able to accommodate 0.2ml/0.1ml PCR tubes as well as microplate 4. The system should support reaction volumes of at least 10µl 5. The system should have ramp rate ≥3.5°C with accuracy of ±0.5°C or less and provision of heating lid to prevent sample evaporation. 6. The system should have capacity to store at least 500 programs in the instrument with provision to store programs in memory stick also 7. The machine should be duly certified/authorized for PCR process and ISO 9001 & CE certified 8. The system should be operable at 220-240v 9. The system should be supplied with at least two years warranty on all parts and on demand repair services as and when required 10. Indian users list for the quoted model along with complete contact details including telephone number and e-mail should be provided 11. Technical features described above should exclusively be supported by authentic original illustrated company catalogue of the instrument/equipment quoted. Photocopy or computer printout of catalogue will not be accepted 12. Vendors business record is to be authenticated by producing income tax returns for at least three consecutive previous years OPTIONAL: 1. The system should be supplied essentially with 2KVa online UPS with backup of at least 1h on full load 2. The system should be supplied with 1.5ton (five star rating) split air conditioning unit with appropriate wall mounted stabilizer (140V – 240V) in order to maintain ambient temperature

No.5-72/2016-17/P&S/Ash

ITEM No. 4

Specification for ELISA READER 1. System must be handy and user friendly. Instrument should be able to work stand-alone also. 2. Automatic calibration before each reading 3. Compatible with various micro-plate platforms (FLAT,U, V-bottomed micro plate or up to 12 well strip plates) 4. Quick reading ability, single and /or dual wavelength selection 5. System must include compatible software for analysis and method performance validation. 6. Works between the wavelength ranges of 400 nm to 750 nm. 7. Photometric range between 0.0 to 3.0 0D and linearity range at least from 0.0to 2.0 0D 8. System must provide accuracy not less than ± 0.01 for 0.0-3.0 0D at different filters and precision of 0.005 0D ( for 0.0-3.0 0D ) 9. High resolution of 0.001 0D 10. Up to 5 Filters slots with filters ( e.g. 415,450,490, 500,655 nm) 11. Fast recording time –not more than 10 sec 12. Data output –USB interface with PCon –board thermal printer (optional) 13. Date storage- Calendar/clock function 14. Software of High throughout, which can run a number assays in a single plate 15. Multiple plates processing with automatic data export. 16. Custom reporting function with performance verification and kinetics analysis. 17. 240 Volt, 50/60 Hz, Stabilizer 0.5 Kva 18. Onsite warranty for at least 2 year. 19. Unit should operate at 220-290v / 50-60 Hz. 20. User’s list & original literature must be submitted Optional: Provided with computer (RAM-4GB, Hard-drive-512MB, Screen size-23’’ LED, CORE i5) for data storage and analysis

No.5-69/2016-17/P&S/Ash

ITEM No. 5 Specification of Elisa Reader & Washer Specification of Elisa Reader

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Detection Mode: Absorbance Light Source: LED Photo detector : Photo diode Plate type: 96 well microplate including strip well microplate. Measurement mode : Endpoint, kinetic and multi-label measurements Measurement Channel : 8 channel Plate Shaking mode Measurement range : 0-4 OD Resolution : 0.001 OD or better Wavelength Range: 400- 750nm Wavelength selection up to 8 filters, Standard filters 405nm, 450nm, 492nm, 620nm. Accuracy: 0.010 OD or better Precision: 0.005 OD or better Linearity : ≤ 1 % or better Full qualitative and quantitative analysis including all major curve fittings and comprehensive QC function. Window based software Display: Operation through PC : i3 processor 4GB RAM, 500GB HDD, 18.5” TFT, Win10., ect., b/w laser printer, UPS (capability to work as standalone or with P.C. Connectivity

Specification of Elisa Plate Washer        

Cross wise aspiration, over flow washing, bottom washing 16 channel manifold (8 channel × 2) Manifold, plate carrier auto cleavable and also can be sterilized 1-4 Wash liquid channel Equipment should be un-pressurized, capable of using any bottle or container. Volume of wash liquid dispensed 50 to 3000µl with 50µl increment Suitable for U,V & flat bottom micro plate etc Residual volume per well