Biographical sketch Name Silvia Fustinoni Address Department of Clinical Sciences and Community Health University of Milano Via S. Barnaba, 8 -20122 Milano e-mail: [email protected] phone + 39 02 50320158 fax + 39 02 50320111

Position Associate Professor

Date of birth 03/18/1963 Education and training (include degree and post-doctoral training) Institution and location

degree

Year (from/to)

Field of study

University of Milano-Italy

Degree in chemistry

Nov 1982/Jan 1988

Chemistry

University of Milano-Italy

Specialization in chemical synthesis

Nov 1989/Nov 1991

Organic synthesis

University of Milano-Italy

Specialization in toxicology

Nov 1994/Jul 1996

Toxicology

University of Padua-Italy

Ph.D.

Nov 1999/Feb 2003

Environmental and Occupational Medicine

Research and professional experience Years(from/to)

institution

position

city

country

Jul 1988-June1992

Farmitalia Carlo Erba

Researcher

Milano

Italy

Jul 1992- Jan 2004

Istituti Clinici di Perfezionamento

Researcher at the laboratory of Toxicology

Milano

Italy

Jun 1995- Dec1995

University of North Carolina at Chapel Hill

Visiting scientist

Chapel Hill

U.S.A.

Feb 2004-Mar 2011

Fondazione Ca’ Granda Ospedale Maggiore Policlinico

Head of the laboratory of Toxicology

Milano

Italy

Mar 2011-Feb 2012

Department of Environmental and Occupational Medicine

Associate Professor

Milano

Italy

LIST OF RELEVANT PUBBLICATIONS 1. Mercadante R, Polledri E, Giavini E, Menegola E, Bertazzi PA, Fustinoni S. Terbuthylazine in hair as a biomarker of exposure, Toxicol Let, 2012, 210: 169-173. 2. Fustinoni S, Rossella F, Polledri E, Bollati V, Campo L, Byun HM, Agnello L, Consonni D, Pesatori AC, Baccarelli A, Bertazzi PA, Global DNA methylation and low-level exposure to benzene. Med Lav, 2012, 103: 84-95. 3. Zhu ZZ, Hou LF, Bollati V, Tarantini L, Marinelli B, Cantone L, Yang AS, Vokonas P, Lissowska J, Fustinoni S, Pesatori AC, Bonzini M, Apostoli P, Costa G, Bertazzi PA, Chow WH, Schwartz J, Baccarelli A. Predictors of global methylation levels in blood DNA of healthy subjects: a combined analysis. Int J Epidem, 2012, 41: 126-139. 4. Carugno M, Pesatori AC, Dioni L, Hoxha M, Bollati V, Albetti B, Byun HM, Bonzini M, Fustinoni S, Cocco P, Satta G, Zucca M, Merlo DF, Cipolla M, Bertazzi PA, Baccarelli A. Increased mitochondrial DNA copy number in occupations associated with low-dose benzene exposure. Environ Health Perspect. 2012; 120:210-5. 5. Fustinoni S, Campo L, Satta G, Campagna M, Ibba A, Tocco MG, Atzeri S, Avataneo G, Flore C, Meloni M, Bertazzi PA, Cocco P. Environmental and lifestyle factors affect benzene uptake biomonitoring of residents near a petrochemical plant. Environ Int. 2012 Feb;39(1):2-7. 6. Campo L, Calisti R, Polledri E, Barretta F, Stopponi R, Massacesi S, Bertazzi PA, Fustinoni S. Assessment of exposure to polycyclic aromatic hydrocarbons in asphalt workers by measurement of urinary 1-hydroxypyrene. Med Lav. 2011 Nov-Dec;102(6):484-93. Italian. 7. Lovreglio P, D'Errico MN, Fustinoni S, Drago I, Barbieri A, Sabatini L, Carrieri M, Apostoli P, Soleo L. Biomarkers of internal dose for the assessment of environmental exposure to benzene. J Environ Monit. 2011 Oct 4;13(10):2921-8. 8. Campo L, Fustinoni S, Bertazzi PA, Quantification of carcinogenic 4-to 6-ring polycyclic aromatic hydrocarbons in human urine by solid-phase microextraction gas chromatography-isotope dilution mass spectrometry. Anal Bioanal Chem 2011, 401: 625-634. 9. Fustinoni S, Campo L, Mercadante R, A quantitative approach to evaluate urinary benzene and Sphenylmercapturic acid as biomarkers of low benzene exposure. Biomarkers, 2011, 16: 334-345. 10. Campo L, Cattaneo A, Consonni D, Scibetta L, Costamagna P, Cavallo DM, Bertazzi PA, Fustinoni S. Urinary methyl tert-butyl ether and benzene as biomarkers of exposure to urban traffic. Environ Int 2011, 37: 404-411. 11. Fustinoni S, Campo L, Mercadante R, Manini P. Methodological issues in the biological monitoring of urinary benzene and S-phenylmercapturic acid at low exposure levels. J Chromatogr B Analyt Technol Biomed Life Sci, 2010, 878: 2534-2540 12. Zhu ZZ, Hou L, Bollati V, Tarantini L, Marinelli B, Cantone L, Yang AS, Vokonas P, Lissowska J, Fustinoni S, Pesatori AC, Bonzini M, Apostoli P, Costa G, Bertazzi PA, Chow WH, Schwartz J, Baccarelli A. Predictors of global methylation levels in blood DNA of healthy subjects: a combined analysis. Int J Epidemiol. 2010 Sep 15. [Epub ahead of print] 13. Fustinoni S, Campo L, Cirla PE, Martinotti I, Buratti M, Longhi O, Foà V, Bertazzi PA, Dermal exposure to polycyclic aromatic hydrocarbons in asphalt workers. Occup Environ Med. 2010 67: 456-463 14. Fustinoni S, Rossella F, Campo L, Mercadante R, Bertazzi PA, Urinary BTEX, MTBE and naphthalene as biomarkers to gain environmental exposure profiles of the general population. Science of the Total Environment 408 (2010) 2840–2849. 15. Fustinoni S, Manini P, Campo L, De Palma G, Andreoli R, Mutti A, Bertazzi PA, Rappaport SM Assessing variability and comparing short-term biomarkers of styrene exposure using a repeated measurements approach. Toxicology Letters 2010 192: 40-44. 16. Campo L, Rossella F, Pavanello S, Mielzynska D, Siwinska E, Kapka L, Bertazzi PA, Fustinoni S Urinary profiles to assess polycyclic aromatic hydrocarbons exposure in coke-oven workers 2010 Toxicology Letters 192:72-78.

17. Hoxha M., Dioni L, Bonzini M, Pesatori AC, Fustinoni S, Cavallo D, Carugno M, Albetti B, Marinelli B, Schwartz J, Bertazzi PA, Baccarelli A Association between leukocyte telomere shortening and exposure to traffic pollution: a cross-sectional study on traffic officers and indoor office workers. Enrironmental Health 2009: 8:41 18. Bukvic N, Lovreglio P, Fanelli M, Susca FC, Ballini A, Lastella P, Foà V, Fustinoni S, Soleo L, Guanti G, Influence of Some Detoxification Enzyme Polymorphisms on Cytogenetic Biomarkers Between Individuals Exposed to Very Low Doses of 1,3-Butadiene, Journal occupational and Environmental Medicine. 2009 51: 811-821. 19. Mattarozzi M, Musci M, Careri M, Mangia A, Fustinoni S, Campo L, Bianchi F. A novel headspace solidphase microextraction method using in situ derivatization and a diethoxydiphenylsilane fibre for the gas chromatography–mass spectrometry determination of urinary hydroxyl polycyclic aromatic hydrocarbons. J Chromatogr A. 2009 1216:5634-5639. 20. Rossella F, Polledri E, Bollati V, Baccarelli A, Fustinoni S. Development and validation of a gas chromatography/mass spectrometry method for the assessment of genomic DNA methylation. Rapid Commun Mass Spectrom. 2009;23(17):2637-46. 21. Rossella F, Campo L, Pavanello S, Kapka L, Siwinska E, Fustinoni S. Urinary polycyclic aromatic hydrocarbons and monohydroxy metabolites as biomarkers of exposure in coke oven workers. Occup Environ Med. 2009;66(8):509-16. 22. Fustinoni S, Mercadante R, Campo L.Self-collected urine sampling to study the kinetics of urinary toluene (and o-cresol) and define the best sampling time for biomonitoring. Int Arch Occup Environ Health. 2009;82(6):703-13. 23. Campo L, Mercadante R, Rossella F, Fustinoni S. 2009 Quantification of 13 priority polycyclic aromatic hydrocarbons in human urine by headspace solid-phase microextraction gas chromatography-isotope dilution mass spectrometry. Anal Chim Acta. 631(2):196-205. 24. Boers D, van Amelsvoort L, Colosio C, Corsini E, Fustinoni S, Campo L, Bosetti C, La Vecchia C, Vergieva T, Tarkowski M, Liesivuori J, Steerenberg P, van Loveren H. 2008Asthmatic symptoms after exposure to ethylenebisdithiocarbamates and other pesticides in the Europit field studies. Hum Exp Toxicol. 27(9):721-7. 25. Swaen G, van Amelsvoort L, Boers D, Corsini E, Fustinoni S, Vergieva T, Bosetti C, Pennanen S, Liesivuori J, Colosio C, van Loveren H. 2008 Occupational exposure to ethylenebisdithiocarbamates in agriculture and allergy: results from the EUROPIT field study. Hum Exp Toxicol. 27(9):715-20. 26. Baranska M, Van Amelsvoort L, Birindelli S, Fustinoni S, Corsini E, Liesivuori J, Van Loveren H. 2008 Association of pesticide exposure, vaccination response, and interleukin-1 gene polymorphisms. Hum Exp Toxicol. 27(9):709-13. 27. Steerenberg P, van Amelsvoort L, Colosio C, Corsini E, Fustinoni S, Vergieva T, Zaikov C, Pennanen S, Liesivuori J, Van Loveren H. 2008. Toxicological evaluation of the immune function of pesticide workers, a European wide assessment. Hum Exp Toxicol. 27(9):701-7. 28. van Amelsvoort L, Mohren D, Slangen J, Swaen G, Corsini E, Fustinoni S, Vergieva T, Bosetti C, Liesivuori J, Tarkowski M, Colosio C, van Loveren H. 2008. Immune effects and exposure to ethylenebisdithiocarbamate pesticides in re-entry workers in the Netherlands. Hum Exp Toxicol. 27(9):693-9. 29. Fustinoni S, Campo L, Liesivuori J, Pennanen S, Vergieva T, van Amelsvoort L, Bosetti C, Van Loveren H, Colosio C. 2008. Biological monitoring and questionnaire for assessing exposure to ethylenebisdithiocarbamates in a multicenter European field study. Hum Exp Toxicol. 27(9):681-91. 30. Primavera A, Fustinoni S, Biroccio A, Ballerini S, Urbani A, Bernardini S, Federici G, Capucci E, Manno M, Lo Bello M. 2008. Glutathione transferases and glutathionylated hemoglobin in workers exposed to low doses of 1,3-butadiene. Cancer Epidemiol Biomarkers Prev.17(11):3004-12. 31. Campo L, Rossella F, Fustinoni S. 2008. Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects. J Chromatogr B Analyt Technol Biomed Life Sci. 875(2):531-40. 32. Fustinoni S, Campo L, Manini P, Buratti M, Waidyanatha S, De Palma G, Mutti A, Foa V, Colombi A, Rappaport SM. 2008. An integrated approach to biomonitoring exposure to styrene and styrene-(7,8)oxide using a repeated measurements sampling design. Biomarkers. 13(6):560-78.

33. Bollati V, Baccarelli A, Hou L, Bonzini M, Fustinoni S, Cavallo D, Byun HM, Jiang J, Marinelli B, Pesatori AC, Bertazzi PA, Yang AS. Changes in DNA methylation patterns in subjects exposed to low-dose benzene. Cancer Res. 2007, 67(3):876-80. 34. Scibetta, Licia; Campo, Laura; Mercadante, Rosa; Foa, Vito; Fustinoni, Silvia. Determination of low level methyl tert-butyl ether, ethyl tert-butyl ether and methyl tert-amyl ether in human urine by HS-SPME gas chromatography/mass spectrometry. Analytica Chimica Acta (2007), 581(1), 53-62. 35. Fustinoni S, Mercadante R, Campo L, Scibetta L, Valla C, Consonni D, Foa V. Comparison between urinary o-cresol and toluene as biomarkers of toluene exposure. J Occup Environ Hyg. 2007, 4(1):1-9. 36. Fustinoni, Silvia; Campo, Laura; Birindelli, Sarah; Colosio, Claudio. Urinary ethylenethiourea as a biomarker of exposure to ethylenebisdithiocarbamates. Methods in Biotechnology (2006), 19(Pesticide Protocols), 79-89. 37. Campo L, Buratti M, Fustinoni S, Cirla PE, Martinotti I, Longhi O, Cavallo D, Foa V. Evaluation of exposure to PAHs in asphalt workers by environmental and biological monitoring. Ann N Y Acad Sci. 2006, 1076:405-20. 38. Lovreglio P, Bukvic N, Fustinoni S, Ballini A, Drago I, Foa V, Guanti G, Soleo L. Lack of genotoxic effect in workers exposed to very low doses of 1,3-butadiene. Arch Toxicol. 2006, 80(6):378-81. 39. L.A. Fanou, T.A. Mobio, E.E. Creppy, B. Fayomi, S. Fustinoni, P. Moller, S. Kyrtopoulos, P. Georgiades, S. Loft, A. Sanni, H. Skov, S. Ovrebo, H. Autrup. Survey of air pollution in Cotonou, Benin-air monitoring and biomarkers. Science of the Total Environment, 2006, 358:85-96. 40. L. Campo, L. Addario, M. Buratti, L. Scibetta, O. Longhi, C. Valla, P.E. Cirla, I. Martinotti, V. Foà, S. Fustinoni, Biological monitoring of exposure to polycyclic aromatic hydrocarbons by determination of unmetabolized compounds in urine. Toxicology Letters, 2006, 162:132-138. 41. C. Colosio, S. Birindelli, L. Campo, S. Fustinoni, M. Tiramani, S. Visentin, M. Maroni. Reference values for ethylenethiourea in urine: results of a pilot study carried out in Northen Italy. Toxicology Letters, 2006, 162:153-157. 42. F.M. Rubino, C. Verduci, M. Buratti, S. Fustinoni, L. Campo, E. Omodeo-Salè, M. Giglio, S. Iavicoli, G. Brambilla, A. Colombi. Assay of urinary α-fluoro-β-alanine by gas chromatography-mass spectrometry for the biological monitoring of occupational exposure to 5-fluorouracil in oncology nurses and pharmacy technicians, Biomedical Chromatography, 2006, 20:257-266. 43. S. Fustinoni, D. Consonni, L. Campo, M. Buratti, A. Colombi, A.C Pesatori, M. Bonzini, P.A. Bertazzi, V. Foà, S. Garte, P.B. Farmer, L.S. Levy, M. Pala, F. Valerio, V. Fontana, A. Desideri, D.F. Merlo. Monitoring low benzene exposure: comparative evaluation of urinary biomarkers, influence of cigarette smoking and genetic polymorphisms. Cancer Epidemiology, Biomarkers and Prevention, 2005, 14: 2237-2244. 44. E. Corsini, S. Birindelli, S. Fustinoni, G. De Paschale, T. Mammone, S. Visentin, C.L. Galli, M. Marinovich, C. Colosio. Immunomodulatory effects of the fungicide Mancozeb in agricultural workers. Toxicology and Applied Pharmacology. 2005, 208:178-185. 45. S. Fustinoni, M. Buratti, L. Campo, A. Colombi, D. Consonni, A.C. Pesatori, M. Bonzini, P. Farmer, S. Garte, F. Valerio, D.F. Merlo, P.A. Bertazzi. Urinary t,t-muconic acid, S-phenylmercapturic acid and benzene as biomarkers of low benzene exposure Chemico-Biological Interactions, 2005, 153-154:253256. 46. P.B. Farmer, B. Kaur, J. Roach, L. Levy, D. Consonni, P.A. Bertazzi, A. Pesatori, S. Fustinoni, M. Buratti, M. Bonzini et al. The use of S-phenylmercapturic acid as a biomarker in molecular epidemiology studies of benzene Chemico-Biological Interactions, 2005, 153-154:97-102. 47. S. Fustinoni, L. Campo, C. Colosio, S. Birindelli, V. Foà, Application of gas chromatographic/mass spectrometry for the determination of urinary ethylenethiourea in humans, J. Chromatography B (2005), 814: 251-258. 48. S. Fustinoni, R. Mercadante, L. Campo, L. Scibetta, C. Valla, V. Foà, Determination of urinary ortho- and meta-cresol in humans by headspace SPME gas chromatography/mass spectrometry, J. Chromatography B (2005), 817: 309-317. 49. S. Fustinoni, L. Perbellini, L. Soleo, M. Manno, V. Foà, Biological monitoring in occupational exposure to low levels of 1,3-butadiene. Toxicology Letters 2004. 149: 353-360.

50. Carrer P., Cavallo D., Fustinoni S., Maroni M., (2003), Dall'igiene e tossicologia industriale all'igiene e tossicologia ambientale: problemi e prospettive. La Medicina del Lavoro. 94: 64-68. 51. S. Fustinoni, L. Soleo, M. Warholm, P. Begemann, A. Rannung, H.-G. Neumann, J.A. Swenberg, L. Vimercati, V. Foà, A. Colombi. Influence of metabolic genotypes on biomarkers of exposure to 1,3butadiene in humans. Cancer Epidemiology, Biomarkers and Prevention 2002. 11: 1082-1090. 52. Fustinoni S., Soleo L., Warholm M., Begemann P., Rannug A., Neumann H.-G., Swenberg J.A., Vimercati L., Foà V., Colombi A. (2002), Influence of metabolic genotypes on biomarkers of exposure to 1,3-butadiene in humans. Cancer, Epidemiology, Biomarkers and Prevention, 11: 1082-1090. 53. Colosio C, Fustinoni S, Birindelli S, Bonomi I, De Paschale G, Mammone T, Tiramani M, Vercelli F, Visentin S, Maroni M (2002) Ethylenethiourea in urine as an indicator of exposure to mancozeb in vineyard workers. 134: 133-140 54. Fustinoni S, Begemann P, Colombi A, Neumann HG, Rannung A, Soleo L, Swenberg JA, Vimercati L, Warholm M, Foà V (2001) Influence by genetic polymorphisms on butadiene-hemoglobin adducts in humans. Toxicology Letters. 123 (Suppl. 1): 29 55. Begemann P, Upton PB, Ranasinghe A, Swenberg JA, Soleo L, Vimercati L, Gelormini A, Fustinoni S, Zwirner-Baier I, Neumann HG (2001) Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and few disel-exposed miners. Chemico-Biological Interactions. 135-136: 675-678 56. Waidyanatha S, Rothman N, Fustinoni S, Smith M, Hayes RB, Bechtold W, Dosemeci M, Guilan L, Yin L, Rappaport SM. (2001) Urinary benzene as biomarker of benzene exposure among occupationally exposed and unexposed subjects. Carcinogenesis 22: 279-286 57. M. Buratti, G. Brambilla, S. Fustinoni, O. Pellegrino, S. Pulvirenti, A. Colombi. Determination of monofluorobimane derivatives of phenylmercapturic and benzylmercapturic acids in urine by highperformence liquid chromatography and fluorimetry. J Chromatogr. B (2001) 751: 305-313. 58. Carrer P, Maroni M, Alcini D, Cavallo D, Fustinoni S, Lovato L, Visigalli F (2000) Assessment through enviromental and biological measurements of total daily exposure to volatile organic compounds of office workers in Milan, Italy. Indoor Air 10: 258-268 59. S. Fustinoni, M. Buratti, R. Giampiccolo, G. Brambilla, V. Foà, A. Colombi. Comparison between blood and urinary toluene as biomarkers of the exposure to toluene. Int. Arch. Occup. Environ. Health (2000) 73: 389-396.