Ce l l C u l t u re
g o es fully automated
Biomek
®
C e l l Wo r k s t a t ion
Cell Analysis Centrifugation Genomics Proteomics Lab Tools Lab Automation Particle Characterisation Bioseparation
Simplify Automate Innovate
Biomek ® Cell Workstation An essential prerequisite in high throughput and high content screening , stem cell research, cell therapy, tissue engineering and production of biopharmaceuticals is the permanent culturing and production of cells. Cell-based assays require cells of consistent quality
Feeding
Seeding
to reduce inter-assay variability.
Cell Maintenance
Production cell lines must be optimized and continuously monitored for consistent quality of their products.
Counting
Maintaining living cells in continuous culture and managing multiple cell lines with different
Passaging
Cell Expansion Harvesting
Counting
requirements in parallel is not an easy task.
Plating
Cell culture is a complex process consisting of
Cell Production
multiple tasks including cell seeding, growing, harvesting, counting and passaging. Processes may run for weeks or months and thus are demanding specifically in terms of maintaining sterility
Pooling
Integrated Biomek® Workstations for research and production have been developed to specifically address automation of cell culture media optimization, hybridoma selection, production cell line optimization and fully automated monoclonal antibody production.
Biomek® Cell Workstation is the latest addition to this range of cellular systems and was developed in cooperation between Celisca and Beckman Coulter.
and tracking all relevant information.
Process to Soluti o n
Software • SAMI® Process Management software to organize parallel and interleaved processes. Includes a calendar view to simplify laboratory resource management.
Biomek® Cell Workstation enables fully automated cell seeding,
• SAMI® Process Definition Editor
growth rate monitoring and quality control, cell harvesting and
enables users to graphically define
plating into microtiter plates.
complex processes consisting
Different cell lines can be cultured in parallel without user inter-
of multiple tasks.
vention over days, weeks or even months. Advanced software control allows true 24/7 operation.
• Intuitive graphic editor to define and schedule individual tasks in
Monolayer Cell Culture Process 10 ml PBS to remove cell culture medium/serum
cell culture flask, confluent monolayer of cell
10 ml buffer
10 ml cell suspension
15 ml cell culture medium per flask 0,5 ml cell suspension in new flasks
max. 20 new cell culture flasks (5x105 cells)
3 microplates, 96 wells, 30.000 cells/well
1 ml trypsin EDTA 9 ml cell culture medium (37°C)
150 l medium per well 33 l cell suspension per well
0,5 ml cell suspension
ce via ll c bil ou ity nti te ng, st
incubation (3-5 days)
10ml buffer
1 ml trypsin/EDTA on cell monolayer to detach cells Incubation (3-10 min, 37°C)
medium
detail. Integrated data tracking
9,5 ml cell suspension (ca. 107 cells)
provides on-the-fly feasibility checking of these tasks. • Pipetting actions can be optimized for any cell type, liquid class and and labware type. • Unique integrated data management, i.e. tracking, logging and reporting. Any information about any sample, well, or plate is registered in a database and available for later use. Labware reports provide information about
Biomek Cell Workstation is based on the Biomek liquid handler
every single plate in the system
and includes new hard- and software components to automate
at any point of time.
®
®
cell culture applications. • Data management system The system can be used with both adherent and suspension
includes SQL viewer for easy
cell cultures and can be easily optimized for different growth
LIMS connectivity.
characteristics. Advanced planning and scheduling tools allow multiple tasks such as cell line expansion and media exchange to occur simultaneously.
Process to So lution The software components of Biomek® Workstation enable users to run and monitor complex processes over weeks and months. Biomek Workstation utilizes the Biomek® liquid handler’s integrated SQL data®
base to ensure integrity of all process steps. Beckman Coulter’s data acquisition and reporting tool is an integral part of any Biomek® Workstation system. Data acquisition and reporting insures data integrity between tasks and utilizes the integrated SQL database. These data management tools allow tracking of locations for every single plate in the system at any
Easy to use...
point of time. SAMI® Process Management Software includes a view of future, current and completed tasks within the system. This interface provides easy access to more detailed reports, including detailed labware reports.
At the same time, the software system provides flexibility for the user to optimize each single step within a process and configure entirely new processes.
... to flexible
Planning and Process Definition and Optimization
SAMI ® Process Definition Editor is an intuitive graphical user interface to map tasks within a complete process. Any SAMI ® EX task is available as a building block for new processes or process variations.
Task Definition and Optimization
Biomek ® Cell Workstation includes Beckman Coulter’s SAMI ® EX optimizing scheduler to define and run individual tasks in any cell cultivation process. New tasks can be developed on a simulation system without interfering with running processes.
Pipetting Optimization
Any pipetting action, e.g speed and tip motion path can be optimized for any type of labware and cell line.
More Options The Biomek® Cell Workstation is a modular system that can be tailored to your specific needs. Systems can range from small footprint workstations for simple cell seeding and feeding to
multiple workstation configurations for complex processes such as fully automated monoclonal antibody production. • Integration of large capacity CO2 Incubators with optional customization for specific environmental control needs • Expanded liquid handling capability including multichannel pipetting for 96- or 384 well plates • Integration of devices for a variety of cell growth characteristics • Integration of high performance plate readers such as Beckman Coulter’s Paradigm™ Detection Platform • Integration of Beckman Coulter‘s Flow Cytometry instruments for high content cell analysis • Integration of analyzers for quality control of biopharmaceutical proteins • Fully automated system for cell fusion in monoclonal antibody production • Options for controlled rate cell freezing and media collection in cell line optimization systems
Biomek ® Cell Works t a t i o n
Biomek® Cell Workstation is based upon Beckman Coulter’s Biomek® Liquid Handler and provides modular integration of components for fully automated cell culture in microplates and automation friendly tissue culture flasks.
www.beckmancoulter.com/labautomat 3D TiltALP for culture flask handling
• Biomek® NXP for liquid handling and labware transport on workstation deck provides small volume handling with high accuracy and precision TiltALP for multiwell plates
• Sterile enclosure with HEPA filtration and laminar flow • Integrated CO2 incubator • Barcode scanning for labware registration and confirmatory reads Barcodes and other labware properties are tracked through the entire process • Integrated Vi-CELL® Cell Viability Analyzer generates cell count
189 slot CO2 incubator for cell growth
and viability data to normalize cell densities in assay plates • Proprietary devices such as the 3D tilting-clamping-tapping ALP • On-deck shaking incubator for trypsinization / cell dissociation procedures • Refrigerated multiple media storage with in-line warming • Integrated high volume rapid bulk media dispense
dual slot shaking incubator
• Self refilling disposable reservoirs for on-deck media supply • Media quality control and quality management
plate lift
tion
Cell Culture g o e s ful ly automated Part No.
Description
CWS 0011
Biomek® Cell Workstation
Biomek® Cell Workstation Specifications Height
2.615 cm
Width
1.800 cm
Depth
1.260 cm
Power Requirements
50/60 Hz, 100-240 VAC
Sufficient to support 181.4 kg (400 lb)
B I O M E K ® P I P E T T I N G P E R F O R M A N C E S P E C I F I C AT I O N S SPAN-8 SYSTEMS Transfer Span-8 Volume Syringe Volume 0.5 µL 250 µL 1 µL 250, 500, 1000 µL 5 µL 250, 500, 1000 µL 10 µL 500 µL 50 µL 500 µL 100 µL 500 µL 250 µL 500 µL 950 µL 1000 µL
Tip Accuracy Types ±% P20, Fixed 60 mm 5 P20, P50, Fixed 60 mm 3 P20, Fixed 60 mm 3 P50, P250 3 P250 3 P250, P1000 3 P1000 2 P1000 1
Precision