Ce l l C u l t u re

g o es fully automated

Biomek

®

C e l l Wo r k s t a t ion

Cell Analysis Centrifugation Genomics Proteomics Lab Tools Lab Automation Particle Characterisation Bioseparation

Simplify Automate Innovate

Biomek ® Cell Workstation An essential prerequisite in high throughput and high content screening , stem cell research, cell therapy, tissue engineering and production of biopharmaceuticals is the permanent culturing and production of cells. Cell-based assays require cells of consistent quality

Feeding

Seeding

to reduce inter-assay variability.

Cell Maintenance

Production cell lines must be optimized and continuously monitored for consistent quality of their products.

Counting

Maintaining living cells in continuous culture and managing multiple cell lines with different

Passaging

Cell Expansion Harvesting

Counting

requirements in parallel is not an easy task.

Plating

Cell culture is a complex process consisting of

Cell Production

multiple tasks including cell seeding, growing, harvesting, counting and passaging. Processes may run for weeks or months and thus are demanding specifically in terms of maintaining sterility

Pooling

Integrated Biomek® Workstations for research and production have been developed to specifically address automation of cell culture media optimization, hybridoma selection, production cell line optimization and fully automated monoclonal antibody production.

Biomek® Cell Workstation is the latest addition to this range of cellular systems and was developed in cooperation between Celisca and Beckman Coulter.

and tracking all relevant information.

Process to Soluti o n

Software • SAMI® Process Management software to organize parallel and interleaved processes. Includes a calendar view to simplify laboratory resource management.

Biomek® Cell Workstation enables fully automated cell seeding,

• SAMI® Process Definition Editor

growth rate monitoring and quality control, cell harvesting and

enables users to graphically define

plating into microtiter plates.

complex processes consisting

Different cell lines can be cultured in parallel without user inter-

of multiple tasks.

vention over days, weeks or even months. Advanced software control allows true 24/7 operation.

• Intuitive graphic editor to define and schedule individual tasks in

Monolayer Cell Culture Process 10 ml PBS to remove cell culture medium/serum

cell culture flask, confluent monolayer of cell

10 ml buffer

10 ml cell suspension

15 ml cell culture medium per flask 0,5 ml cell suspension in new flasks

max. 20 new cell culture flasks (5x105 cells)

3 microplates, 96 wells, 30.000 cells/well

1 ml trypsin EDTA 9 ml cell culture medium (37°C)

150 l medium per well 33 l cell suspension per well

0,5 ml cell suspension

ce via ll c bil ou ity nti te ng, st

incubation (3-5 days)

10ml buffer

1 ml trypsin/EDTA on cell monolayer to detach cells Incubation (3-10 min, 37°C)

medium

detail. Integrated data tracking

9,5 ml cell suspension (ca. 107 cells)

provides on-the-fly feasibility checking of these tasks. • Pipetting actions can be optimized for any cell type, liquid class and and labware type. • Unique integrated data management, i.e. tracking, logging and reporting. Any information about any sample, well, or plate is registered in a database and available for later use. Labware reports provide information about

Biomek Cell Workstation is based on the Biomek liquid handler

every single plate in the system

and includes new hard- and software components to automate

at any point of time.

®

®

cell culture applications. • Data management system The system can be used with both adherent and suspension

includes SQL viewer for easy

cell cultures and can be easily optimized for different growth

LIMS connectivity.

characteristics. Advanced planning and scheduling tools allow multiple tasks such as cell line expansion and media exchange to occur simultaneously.

Process to So lution The software components of Biomek® Workstation enable users to run and monitor complex processes over weeks and months. Biomek Workstation utilizes the Biomek® liquid handler’s integrated SQL data®

base to ensure integrity of all process steps. Beckman Coulter’s data acquisition and reporting tool is an integral part of any Biomek® Workstation system. Data acquisition and reporting insures data integrity between tasks and utilizes the integrated SQL database. These data management tools allow tracking of locations for every single plate in the system at any

Easy to use...

point of time. SAMI® Process Management Software includes a view of future, current and completed tasks within the system. This interface provides easy access to more detailed reports, including detailed labware reports.

At the same time, the software system provides flexibility for the user to optimize each single step within a process and configure entirely new processes.

... to flexible

Planning and Process Definition and Optimization

SAMI ® Process Definition Editor is an intuitive graphical user interface to map tasks within a complete process. Any SAMI ® EX task is available as a building block for new processes or process variations.

Task Definition and Optimization

Biomek ® Cell Workstation includes Beckman Coulter’s SAMI ® EX optimizing scheduler to define and run individual tasks in any cell cultivation process. New tasks can be developed on a simulation system without interfering with running processes.

Pipetting Optimization

Any pipetting action, e.g speed and tip motion path can be optimized for any type of labware and cell line.

More Options The Biomek® Cell Workstation is a modular system that can be tailored to your specific needs. Systems can range from small footprint workstations for simple cell seeding and feeding to

multiple workstation configurations for complex processes such as fully automated monoclonal antibody production. • Integration of large capacity CO2 Incubators with optional customization for specific environmental control needs • Expanded liquid handling capability including multichannel pipetting for 96- or 384 well plates • Integration of devices for a variety of cell growth characteristics • Integration of high performance plate readers such as Beckman Coulter’s Paradigm™ Detection Platform • Integration of Beckman Coulter‘s Flow Cytometry instruments for high content cell analysis • Integration of analyzers for quality control of biopharmaceutical proteins • Fully automated system for cell fusion in monoclonal antibody production • Options for controlled rate cell freezing and media collection in cell line optimization systems

Biomek ® Cell Works t a t i o n

Biomek® Cell Workstation is based upon Beckman Coulter’s Biomek® Liquid Handler and provides modular integration of components for fully automated cell culture in microplates and automation friendly tissue culture flasks.

www.beckmancoulter.com/labautomat 3D TiltALP for culture flask handling

• Biomek® NXP for liquid handling and labware transport on workstation deck provides small volume handling with high accuracy and precision TiltALP for multiwell plates

• Sterile enclosure with HEPA filtration and laminar flow • Integrated CO2 incubator • Barcode scanning for labware registration and confirmatory reads Barcodes and other labware properties are tracked through the entire process • Integrated Vi-CELL® Cell Viability Analyzer generates cell count

189 slot CO2 incubator for cell growth

and viability data to normalize cell densities in assay plates • Proprietary devices such as the 3D tilting-clamping-tapping ALP • On-deck shaking incubator for trypsinization / cell dissociation procedures • Refrigerated multiple media storage with in-line warming • Integrated high volume rapid bulk media dispense

dual slot shaking incubator

• Self refilling disposable reservoirs for on-deck media supply • Media quality control and quality management

plate lift

tion

Cell Culture g o e s ful ly automated Part No.

Description

CWS 0011

Biomek® Cell Workstation

Biomek® Cell Workstation Specifications Height

2.615 cm

Width

1.800 cm

Depth

1.260 cm

Power Requirements

50/60 Hz, 100-240 VAC

Sufficient to support 181.4 kg (400 lb)

B I O M E K ® P I P E T T I N G P E R F O R M A N C E S P E C I F I C AT I O N S SPAN-8 SYSTEMS Transfer Span-8 Volume Syringe Volume 0.5 µL 250 µL 1 µL 250, 500, 1000 µL 5 µL 250, 500, 1000 µL 10 µL 500 µL 50 µL 500 µL 100 µL 500 µL 250 µL 500 µL 950 µL 1000 µL

Tip Accuracy Types ±% P20, Fixed 60 mm 5 P20, P50, Fixed 60 mm 3 P20, Fixed 60 mm 3 P50, P250 3 P250 3 P250, P1000 3 P1000 2 P1000 1

Precision