School of Allied Health Sciences and Public Health Walailak University

Telephone: 0-75672104, 0-75672105 Fax: 0-75672106 E-mail: [email protected] Homepage: http://ihh.wu.ac.th

Research Abstracts 2006-2007

Walailak University

Antiseptic Activity of Cosmetic Products Against Microorganisms Chuchard PUNSAWAD, Athisa INTHARAKIRI, Onuma PRATUMSUWAN and Monthon LERTCANAWANICHAKUL School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected]) The study on antimicrobial activity in 34 sample of the cosmetic products showed that 25 sample (75 %) had antimicrobial activity by paper disk diffusion method. These sample were 11 solids (44 %), 6 liquids (24 %), 2 powder (8 %) and 6 gels (24 %). They exhibited antimicrobial activities against Escherichia coil, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and candida albicans in the percentage of 32.3, 0, 61.8, 67.6 and 17.6, respectively. The cosmetic products which had Triclosan or zinc Pyithione can show antiseptic activity against bacteria or fungi, respectively. The minimum inhibitory concentration (MIC) of represent of cosmetic products were examined in solids (soap) No. 3 S4, liquid (shampoo) No. L3, powder (talc) No.P3, and gel (toothpaste) No. G4 were 1:320, 1:1280, 1:40 and 1:1280, respectively, by means of paper disk diffusion method. Keywords: Grant: Publisher:

Autoregressive integrate moving average (ARIMA) model, dengue heamorrhagic fever (DHF) Walailak University Thailand Journal of Health Promotion and Environmental Health 2007; 30(20), 39-50.

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Research Abstracts 2006-2007

Walailak University

Prevalent of Metabolic Syndrome in Walailak University Personal Receiving Annual Health Examinations Jitbanjong TANGPONG1, Uthai TRIAPIRUK1, Warangkana CHUNGLOK1 and Dararat PANVONG2 1

Medical Technology Program, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand 2 Center for Scientific and Technological Equipment, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected])

Metabolic syndrome (MetS) are increased risk for developing diabetes mellitus and cardiovascular disease which include high blood pressure (BP), elevated triglycerides, low concentrations of high-density lipoprotein (HDL-c), impaired glucose tolerance, and central obesity. The present study we sought to estimate the prevalence of MetS among Walailak University personals receiving annual health examination.The cross-sectional study of 304 participants (115 men and 189 women) aged between 25 65 years who participated in annual health examinations at Walailak University Medical Laboratory Center (WU-MeT). The present study was conducted between August and September 2006. The National Cholesterol Educational Program Adult Treatment Panel III (NCEP-ATP III) criteria were used to estimate the prevalence of MetS, in which; 1) waist circumstance (WC) >80 cm. for women and >90 cm for men, 2) high blood pressure (BP) >130/85 mm.Hg, 3) hypertriglyceridemia >150 mg/dL., 4) low HDLcholesterol 0.05). This suggested that “non-smokers but close to smokers” will receive carbon monoxide gas equivalent to those “smokers that smoke less than 10 cigarettes per day.” Keyword: Grant: Published:

Carboxyhemoglobin, derivative spectrophotometry, smokers, non-smokers Walailak University Journal of the Medical Technologist Association of Thailand 2007; 35(3), 2115-23. 51

Research Abstracts 2006-2007

Walailak University

Evaluation of Commercially Micral® Test Strips for Determination of Microalbumin in Type 2 Diabetes Millitus Uthai TRIAPIRUX1, Chatchai RUTTANAMAD2, Akekalak KHADNANTA2 and Benchamas RAHEDHARN2 1

School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand 2 Medical Technology Student, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected]) Background: Microalbuminuria is the earliest clinical finding for renal disease and a risk factor for hypertensive cardiovascular disease in type 2 diabetes millitus. Several methods, including many immunoassays have been applied for detection of microalbuminuria. Among these is a semi - quantitative commercially Micral® test strips which is widely use in clinical laboratory. The purpose of this study is to evaluate Micral® test strips as a rapid and accurate method for detecting microalbuminuria in type 2 diabetic patients. Method: In this study, a total of 120 urine samples of type 2 diabetic patients were obtained from Maharaj Nakhon Si Thammarat hospital for analysis. Urinary albumin concentrations were determined using Micral®test strips and compared to results measuring albumin by the standard sulfosalicylic acid turbidimetric method. Results: The performance characteristic of Micral® test strips for detecting microalbuminuria in type 2 diabetic patients were accuracy 90 %, sensitivity 95 %, specificity 75 %, positive predictive value 92 % and negative predictive value 81 %. Conclusion: This assay has been applied to detection of microalbumin in urine samples of type 2 diabetic patients, with simplicity, rapidity, high sensitivity and good selectivity. Keywords: Grant: Presented:

Microalbumin, microalbuminuria, Micral®test strips, evaluation The 33rd Congress on Science and Technology of Thailand (STT 33) 18-20 October 2007, Walailak University, Nakhon Si Thammarat, Thailand.

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Research Abstracts 2006-2007

Walailak University

Use of an Ion-Selective Electrode for Copper Measurement in Swimming Pool Water of Walailak University Wachirawan PIMROTE1, Napatt TUSSANAWISUT2, Uthai TRIAPIRUX3, Saravut DEJMANEE4 and Roongroje RATANA-OHPAS4 1

School of Science and Technology, Naresuan University, Phayao Campus, Phayao 56000, Thailand 2 Center for Scientific and Technological Equipment, Walailak University, Nakhon Si Thammarat 80161, Thailand 3 School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand 4 Division of Chemistry, School of Science, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected])

The analysis of copper in swimming pool water by using potentiometry with copper ion-selective electrode (Cu2+-ISE) has been studied. The measurement concentration of copper by ISE potentiometry gave a linearity between 10-6 to 10-2 M (0.06 - 635.5 ppm) with r2 was 0.9999, Nernstian slope ranged from 28 to 30 mV/decade and relative standard deviation (RSD) was less than 0.51 %. The detection limit was found to be 3 × 10-7 M (0.02 ppm) and percentage recoveries varied between 90 to 112. The proposed method was applied to determine the amount of copper(II) in water samples collected from swimming pools were ranged from 1.17 × 10-6 to 2.83 × 10-5 M (0.07 - 1.80 ppm). Keywords: Grant: Presented:

Copper, swimming pool water, ISE The 33rd Congress on Science and Technology of Thailand (STT 33) 18-20 October 2007, Walailak University, Nakhon Si Thammarat, Thailand.

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Research Abstracts 2006-2007

Walailak University

Diabetes Risk Assessment in Ron Phibun, Southern Thailand Wissarut KHONNGERN, Penchom JUNWAN, Patchara RATTANAPORN, Weena BANGMOUNG-GAM, Mariya BILLEAH, Amonrat NOOKLIENG, Sukanya SANGDEE, Hamidah WAEBUERAHING, Supaporn YANGNGAM, Patcharin PANSOMBUN, Tanrat SAKSRI, Junporn PLASINGHA, Sommarat THANAVANITNAM and Warangkana CHUNGLOK Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected]) Diabetes has been predicted as remarkable increase in Thailand. Early recognition to prevent and delay the development of diabetes will be beneficial. Diabetes prediction is a useful tool to identify people who are likely to develop diabetes. The pilot study was aimed to determine the percentages of people who were at risk of diabetes. Diabetes risk scoring system was obtained from the published data. Data were taken from residents in Ron Phibun District, Nakhon Si Thammarat Province. The study included 81 subjects which were 30 and 51 of males and females, respectively. Results showed that there were 60 % of males and 62.75 % of females had diabetes risk scores ≥ 6. It was considered to have increased incident of diabetes in the near future. Diabetes risk assessment may be used to predict high-risk people and thus leading to preventive strategies by receiving health education and having lifestyle modification. Keywords: Grant: Presented:

Diabetes, risk assessment School of Allied Health Sciences and Public Health, Walailak University / Health Promotion Public Health Faculty The 33rd Congress on Science and Technology of Thailand (STT 33) 18-20 October 2007, Walailak University, Nakhon Si Thammarat, Thailand.

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Research Abstracts 2006-2007

Walailak University

Epidermiology of Burkholderia pseudomallie in Nakhon Si Thammarat and Nearby Area Wiyada KWANHIAN School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand (E-mail: [email protected]) Melioidosis disease is caused by Burkholderia pseudomallei which is a saprophytic gram negative bacterium. B. Pseudomallei can survive in environmental condition such as soil water and dust. However, the objective of this study is to isolate B. Pseudomallei from environmental sources including, soil of rubbertree field, soil of orchard, soil of rice field and water from natural source. The present study 511 total samples, Identification result by conventional biochemical test found 17 strains of B. Pseudomallei (3.32 %). It could be distinguished to 4.39 % of soil from rubbertree field (5 from 114 samples), 1.42 % of soil from orchard (2 from 141 samples), 8.33 % of soil from rice field (8 from 96 samples) and 1.25 % of water (2 from 116 samples). Additionally, the virulence of an epidemic B. Pseudomallei by using L-arabinose assimilation test found that all 17 strains from environment sources are Ara+ strains which is different from Clinical strain (Ara−). These research data could encourage an advantage to epidemiology field, especially in melioidosis basic information in Nakhon Si Thammarat and closed area and also in south of Thailand. However, the evidence and virulence of an epidemic B. Pseudomallei has not been completely reported in Nakhon Si Thammarat and nearby area. Keywords: Grant: Published:

Burkholderia pseudomallei, melioidosis, L-arabinose assimilation Institute of Research and Development, Walailak University Journal of the Medical Technologist Association of Thailand 2006; 34(3), 1667-74.

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