Research article. Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, ISSN

Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406 Journal of Biotechnology and Biosafety Research a...
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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

Journal of Biotechnology and Biosafety

Research article COMPARATIVE ASSAY OF ESCITALOPRAM IN DIFFERENT MEDIA __________________________________

Safila Naveed1, Fatima Qamar,1, Syeda Sarah Abbas1,2 Sania Zehra1, Sehrish Kirn 1, Zohra Barkat1, Syeda Zainab2 ___________________________________________________ 1

Faculty of Pharmacy, Jinnah University for women Karachi 2 Faculty of Pharmacy, University of Karachi 3 Faculty of Pharmacy, Federal Urdu University

Corresponding Author Email: [email protected], [email protected]

ABSTRACT Escitalopram is in a class of antidepressants called selective serotonin reuptake inhibitors (SSRIs). It works by increasing the amount of serotonin, a natural substance in the brain that helps maintain mental balance. Escitalopram is used to treat depression and generalized anxiety disorder (GAD); excessive worry and tension that disrupts daily life and lasts for 6 months or longer. A simple, efficient spectrophotometric method for the assay of escitalopram has been developed. Comparison of four different brands of escitalopram (Cipralex, Depsit, Es-pramcit and Morcet) has also been done. The assay is carried out in lambda max at about 244nm using water as solvent. Different formulations of drug were dissolved in water to prepare solutions containing escitalopram 20mg. Similarly, a sample of ground tablets of different brand were dissolved in water and dilutions were made in the range of 200ppm, 100ppm, 50ppm and 25ppm. The absorbance of sample preparation was measured at 244nm against the water (blank solvent) and the assay was determined by the absorbance of each brand. Our result reveals that among all the four brands of escitalopram show linearity and squared correlation. Coefficient values of all the brands of escitalopram are well within the limit and also concluded that its absorption is more in empty stomach.

KEYWORDS: Escitalopram, UV spectrophotometry Assay. _______________________________________________________________________________________ INTRODUCTION: Escitalopram is an antidepressant that is belong to the class (SSRI) selective serotonin re-uptake inhibitor .its IUPAC name is (1S)-1-[3-(dimethylamino) propyl]-1-(4fluorophenyl)-3H-2-benofuran-5-carbonitrile, molecular formula is C20H21FN20 having molecular mass of 324.391943g/mol. (Baldwin et al., 2007). This is freely soluble in dimethyl sulfoxide, soluble in isotonic saline and sparingly soluble in ethanol and water. (Moore N et al., 2005). It is slightly soluble in ethyl acetate and insoluble in hepatane. It is used in the treatment of anxiety, dysmorphic disorders and depression associated with mood disorders. Half life of Escitalopram is 27-32 (Boulenger JP et al., 2006). The antiobsessive-compulsive ,antidepressant and www.jobb.co.in

antibulimic action is because that it inhibits the CNS neural reuptake of serotonin , it blocks the reuptake at the serotonin reuptake pump of the neuronal membrane, and enhance the actions of serotonin on 5HT1A auto receptors (Bielski RJ et al., 2004). Drugs that include in class of SSRI has less affinity of binding with acetylcholine, histaminee, and nor epinephrine receptor as compare to TCA (tricyclic antidepressants). (Nierenberg AA et al., 2007). The absolute bioavailability of citalopram is about 80% relative to an intravenous dose. Following oral administrations of escitalopram, the fraction of drug recovered in the urine as escitalopram 8% and S-demethylcitalopram (S-DCT) 10%. Clearance of escitalopram after oral administration is 600 mL/min, approximately 7% of drug due to renal clearance (Plenge P et al., 2007). Metabolites of Escitalopram are S-

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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

Journal of Biotechnology and Biosafety

didemethylcitalopram (S-DDCT) and S-DCT(Sdemethylcitalopram).its Vd (Volume of Distribution) is 12 L/kg. Metabolize mainly through liver (hepatic metabolism). Metabolism of escitalopram undergoes due to CYP3A4 and CYP2C19 enzymes that are present in liver (Pollock BG 2001).

After preparation of API and tablet solutions, in pH1 and pH4 and strength of solution 200ppm, 100ppm, 50ppm and 25ppm in 100mL absorbance of the sample preparation and standard preparation in 1cm cell at the wavelength of maximum absorbance at about 244 nm, using a spectrophotometer, using the water as a blank solution.

EXPERIMENTAL METHODOLOGY:

RESULT AND DISCUSSION

Material and reagents Glass wares, beakers, volumetric flask, measuring cylinder, pipette and stirrer were used. All glass wares were washed and rinsed with double distilled water. Reagents used were as follows 0.1N hydrochloric acid and de-ionized water or double distilled water. All the Reagents were of Analytical grade. Instruments UV visible spectrophotometer model 1601 Shimadz u as used to measurement of spectra. The solvents, which were used for the preparation of sample for assay was water. Preparation of pH 1 and pH 4 solution: (Safila Naveed et al., 2015). Methods Preparation of simulated gastric juice and buffers 0.1 N hydrochloric acid was prepared by diluting 9mL hydrochloric acid of analytical grade (11 N) in a liter volumetric flask and the volume was made up to the mark with de-ionized water. Sample preparation (Safila Naveed et al., 2015) The four different brands (Cipralex, Depsit, Es-pramcit and Morcet) were purchased from medical store located in Karachi, Pakistan. All tablets which were labeled to contain escitalopram 20mg per tablet. 20 tablets of four different brand of escitalopram (Cipralex, Depsit, Es-pramcit and Morcet) from the marketed sample were weighed and crushed with the help of a mortar and pestle. By calculating the average weight of each brand equivalent to 20mg of escitalopram was transferred into a volumetric flask containing approx 100 mL water. Then make up volume up to 100mL with water.

Pharmaceutical assay was carried out by using spectrophotometer on all brands of escitalopram tablets during the study. Table 1 shows name brand and absorbance of different brands. Four different brands of Escitalopram (Cipralex, Depsit, Es-pramcit and Morcet) is taken and their Solutions in pH 1 and pH 4 and solution of 200ppm, 100ppm, 50ppm and 25ppm is prepared. Their percent assay is calculated and regression equation and regression line is obtained to predict further availability of drug. Their maximum absorption is checked in different media. We observe that Escitalopram is highly absorbed in empty stomach ie., pH 1. For detect linearity solutions of 200ppm, 100ppm, 50ppm and 25ppm is prepared and three absorbances in triplicate were taken at each level in spectrophotometric analysis. For linearity plot concentration vs. absorbance at level 200ppm, 100ppm, 50ppm and 25ppmof each brand is shown in fig 1, fig 2, fig 3, fig 4, fig 5 and fig 6. Squared correlation coefficient of each brand is shown in table no 2. It should not be less than 0.99. Squared correlation coefficient values of all the brands of escitalopram are well within the limit. All four brands of escitalopram showed linear relation with their dilution.

Dilutions From the sample solution of different brands of escitalopram different dilutions are made. Solutions of 200ppm, 100ppm, 50ppm and 25ppm of all the three brands were prepared. After preparation of standard and capsule solutions, strength of solution 200ppm in 100mL Procedure: www.jobb.co.in

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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

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Table 1: Absorbance of different brand of escitalopram in different medium TABLETS

Water

Ph1

Ph4

200ppm

100ppm

50ppm

25ppm

A= Cipralex

2.705

2.623

2.536

2.705

2.392

1.631

0.871

B=Depsit

2.709

2.642

2.543

2.709

2.456

1.769

1.005

C= Es-pramcit

2.761

2.663

2.588

2.761

2.464

1.918

1.099

D= Morcet

2.701

2.627

2.566

2.701

2.539

1.701

0.963

Table 2: Regression equation and Correlation Coefficient of different brand of escitalopram BRANDS STRENGHT

STRENGTH

A= Cipralex B=Depsit C= Es-pramcit D= Morcet

20mg 20mg 20mg 20mg

REGRESSION EQUATION Y = 0.0218x + 0.311 Y = 0.0216x + 0.4221 Y = 0.021x + 0.5213 Y = 0.023x + 0.3352

CORELATION COEFFICIENT 0.9624 0.9431 0.9052 0.965

Fig 1:Linearity plot for assay of different dilutions of Cipralex

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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

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Fig 2:Linearity plot for assay of different dilutionss of Depsit

Fig 3:Linearity plot for assay of different dilutions of Es-pramcit

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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

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Fig 4:Linearity plot for assay of different dilutions of Morcet

Figure 5: Absorbance of different brand of escitalopram in different medium CONCLUSION Using spectrophotometer on all brands of escitalopram during the study carried out pharmaceutical assay. A good linear relationship was observed for (Cipralex, Depsit, Es-pramcit and Morcet) the concentration ranges of 200ppm, 100ppm, 50ppm and 25ppm. We observe that Escitalopram is highly absorbed in empty stomach ie.,pH 1.This method can be used for routine analysis in pharmaceutical industry as this method is simple and less time consuming. www.jobb.co.in

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Journal of Biotechnology and Biosafety Volume 3, Issue 3, March-April 2015, 237-242 ISSN 2322-0406

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REFERENCES Baldwin, David S, Reines, ElinHeldbo, Guiton, Christina, Weiller, Emmanuelle. (2007) "Escitalopram therapy for major depression and anxiety disorders." The Annals of Pharmacotherapy 41(10): 1583-92. Moore N, Verdoux H, Fantino B(2005). Prospective, multicentre, randomized, double-blind study of the efficacy of escitalopram versus citalopram in outpatient treatment of major depressive disorder. IntClinPsychopharmacol.20 (3): 131-7. Boulenger JP, Huusom AK, Florea I, Baekdal T, Sarchiapone M(2006). A comparative study of the efficacy of long-term treatment with escitalopram and paroxetine in severely depressed patients. Curr Med Res Opin. 22(7): 1331-41. Bielski RJ, Ventura D, Chang CC (2004). A doubleblind comparison of escitalopram and venlafaxine extended release in the treatment of major depressive disorder. J Clin Psychiatry. 65(9): 1190-6.

Nierenberg AA, Greist JH, Mallinckrodt CH, Prakash A, Sambunaris A, Tollefson GD, Wohlreich MM (2007). Duloxetine versus escitalopram and placebo in the treatment of patients with major depressive disorder: onset of antidepressant action, a non-inferiority study. Curr Med Res Opin. 23(2): 401-16. Plenge P, Gether U, Rasmussen SG.(2007). Allosteric effects of R- and S-citalopram on the human 5-HT transporter: evidence for distinct high- and low-affinity binding sites. Eur J Pharmacol.567:1–9. Pollock BG.(2001). Citalopram: a comprehensive review. Expert Opin Pharmacother. 2:681–698. Safila Naveed, Fatima Qamar ,Syeda Zainab (2015). , Effect of Acidic and base on Nifidipine by using UV Spectrophotometer International journal of Pharma Science and research. 6:113-116. Safila Naveed, Fatima Qamar ,Syeda Zainab ,Ghulam Sarwar, Khan Usmanghani and Tanweer Alam (2015). , Effect of Acidic and Alkaline Medium on Fexofenadine brands using UV Spectrophotometer Canadian journal of Applied Science. 5:100-104.

Citation of this article: Safila Naveed, Fatima Qamar,Syeda Sarah Abbas, Sania Zehra, Sehrish Kirn , Zohra Barkat Syeda Zainab. COMPARRATIVE ASSAY OF ESCITALOPRAM IN DIFFERENT MEDIA. Journal of Biotechnology and Biosafety. 3(3): 237-242

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Conflict of Interest: None Declared

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