Processed Supernatants from Lactobacillus Rhamnosus GG Culture Improve Intestinal Barrier Function in Rats after Massive Small Bowel Resection
Processed Supernatants from Lactobacillus Rhamnosus GG Culture Improve Intestinal Barrier Function in Rats after Massive Small Bowel Resection
Jiang ...
Processed Supernatants from Lactobacillus Rhamnosus GG Culture Improve Intestinal Barrier Function in Rats after Massive Small Bowel Resection
Jiang Wu, Linxi Qian, Yan Zhong, Wei Cai Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai Institute for Pediatric Research Shanghai, China
I have the following financial relationships to disclose: Company: Mead Johnson Nutrition
No Products or services was tested in this study, which was partly financially supported by MJN.
Background Short bowel syndrome (SBS) and gut barrier dysfunction – Malabsorption state when the functioning gut mass is below the amount necessary for adequate digestion and absorption – Association with gut barrier dysfunction, intestinal bacterial overgrowth and translocation
Probiotics and gut barrier function – Beneficial effects of live probiotics, e.g. LGG on gut barrier function, BUT with safety concern in vulnerable population, e.g. SBS. – As an alternative, probiotic bacteria derived nonviable products or metabolites might mimic the beneficial effects of live probiotics – Soluble mediator from LGG shows immune modulatory properties.
Piena-Spoel et al. 2000, Camilleri et al. 2012, Reddy et al. 2013 Al-Sadi et al. 2013, Kunz et al. 2004, Sentongo et al. 2008, Donato et al. 2010
Claudin-3 expression (mice) Patel et al. 2012
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Experimental Setup To study effects of nutritional interventions on gut barrier function in SBS Sprague Dawley (5-6wk old) (n=8/group)
Sacrifice
Food intake, body weight Bowel transection with re-anastomosis
Chow + PBS (sham) Chow + PBS (SBS)
75% -80% small bowel resection
Chow + LGG soluble mediator (LEGa, column)
1. Intestinal morphology/adaptation 2. Systemic and local inflammation 3. Bacterial translocation 4. Intestinal barrier function - Tight Junction proteins - intestinal permeability - sIgA
Chow + LGG soluble mediator (LEGb, ultrafiltr.)
Chow + viable LGG Day 15
Day 1
oral gavage: 5 x 108 CFU (equivalent) LGG/day
LGG soluble mediator preparation: Standardized proprietary production protocol (controlled fermentation process, collection of spent medium, downstream processing: column chromatography (LEGa) or ultrafiltration (LEGb), sterile filtration, drying)
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LGG Soluble Mediators in SBS Improved body weight gain
