University of Rhode Island

DigitalCommons@URI Pharmacy Practice Faculty Publications

Pharmacy Practice

2015

Observed antagonistic effect of linezolid on daptomycin or vancomycin activity against biofilmforming methicillin-resistant Staphylococcus aureus in an in vitro pharmacodynamic model Megan Luther Pharm. D. University of Rhode Island

Kerry L. LaPlante Pharm. D. University of Rhode Island, [email protected]

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Citation/Publisher Attribution Luther M and LaPlante KL. (2015). "Observed antagonistic effect of linezolid on daptomycin or vancomycin activity against biofilmforming methicillin-resistant Staphylococcus aureus in an in vitro pharmacodynamic model." Antimicrobial Agents and Chemotherapy. Available at: http://aac.asm.org/content/early/2015/09/09/AAC.01604-15.abstract

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Title: Observed antagonistic effect of linezolid on daptomycin or vancomycin activity against

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biofilm-forming methicillin-resistant Staphylococcus aureus in an in vitro pharmacodynamic

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model

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Date August 19, 2015

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Running title: Linezolid antagonism of cell wall active agents

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Megan Luther1,2 and Kerry L. LaPlante1,2,3#

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1. Rhode Island Infectious Diseases (RIID) Research Program, Veterans Affairs Medical Center,

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Providence, RI

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2. Department of Pharmacy Practice, University of Rhode Island, Kingston, RI

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3. Warren Alpert Medical School, Brown University, Providence, RI

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#

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Rhode Island, Department of Pharmacy Practice; Senior Director, RIID Research Program,

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Providence Veterans Affairs Medical Center; Adjunct Associate Professor of Medicine, Brown

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University; University of Rhode Island, College of Pharmacy; 7 Greenhouse Road; Kingston, RI

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02881; office: 401.874.5560; fax: 401.874.2717; email: [email protected]

Corresponding Author: Kerry L. LaPlante, Pharm.D., Professor of Pharmacy, University of

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ABSTRACT

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Pharmacodynamic activity in antibiotic combinations of daptomycin, vancomycin and linezolid

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was investigated in a 48h in vitro pharmacodynamic model. Using free human-simulated

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concentrations, activity against clinical biofilm-forming methicillin-resistant Staphylococcus

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aureus isolates was evaluated. Linezolid antagonized vancomycin activity at 24 and 48h.

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Linezolid antagonized daptomycin at 24 and 48h depending on dose and strain. Adding

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daptomycin increased vancomycin activity at 48h (p