Monitoring Engraftment Post Hematopoietic Stem Cell Transplantation David Senitzer, Ph.D Director, HLA Laboratory City of Hope National Medical Center Duarte, California 2009

Outline • Chimerism monitoring in hematopoietic cell transplantation (HCT) • Overview of methodologies • Short Tandem Repeat (STR) loci • Multiplex STR analysis • Identification of Informative Alleles • Chimerism monitoring in HCT • Q-PCR – a new method for chimerism analysis City of Hope

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Clinical Relevance Engraftment following hematopoietic cell transplantation

Detection of relapse/rejection

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Goals of Monitoring Chimerism • To determine the presence or absence of a recipient cell population post transplantation. • To quantitate the relative proportion of the recipient and donor cell populations. • If possible, to identify a trend of an increasing proportion of recipient cells. City of Hope

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What is needed for clinically relevant analysis? • Panel of genetic loci sufficient to differentiate donors from recipients • Quick turn-around-time (