Cherokee CRC, LLC 10838 E Marshall Street Suite 220 Tulsa, Oklahoma 74116
MOLD SAMPLING ANALYSIS REPORT
For
Cherokee Nation Entertainment Corporate Accounting Office Tahlequah, OK
Introduction Cherokee CRC, LLC, conducted a mold and asbestos sampling investigation in the Cherokee Nation Entertainment Corporate accounting office located on Hwy 51 near Tahlequah, OK. The sampling was conducted on December 19th, 2014, January 16th, 2015, and January 19th, 2015. Work was performed at the request of Joe Washum, Director of Safety and Environmental for Cherokee Nation Businesses and Kevin Ogle, Director of Construction for Cherokee Nation Businesses. The purpose of the sampling was to determine if any unacceptable concentrations of mold spores exist in the sampled locations that could be negatively impacting employee health and to determine if any materials in the complaint area contained asbestos containing materials. Cherokee CRC, LLC coordinated with Mr. Washum and Matthew McCrary (CNE Safety and Environmental) to conduct the sampling and to access the areas of investigation. The sampling was conducted in a manner to minimize any disturbance of the mold colonies present inside the building while providing maximum safety for project personnel and employees present during the sampling.
Sampling Methodology The initial sampling took place on December 19th at the facility during a walkthrough with Kevin Ogle and Joe Washum. The samples collected at this time included possible asbestos containing materials within the building, more specifically, in the primary complaint area identified as Treva Fields’ office. Five (5) samples were collected from suspect materials that could possibly contain asbestos fibers. These samples included the complaint office’s wallboard around the window sample 001, the baseboard and mastic sample 002, the carpet mastic 003, the brownish yellow attic insulation present throughout the building, and a sample of the vermiculite from the wall cavity. Two (2) tape lift mold samples were collected from the complaint area (Treva Fields’ Office) on December 19th. These samples included suspect white residue on the brick inner wall, and a rusty red spot on the carpet at the base of the southern interior wall. Additional mold sampling occurred on January 16th, 2015. John Freise met with Mr. McCrary who arranged access to the building for after-hours sampling as not to disturb employees who working in the building. However, access was not available to the complaint area of the building on the 16th so only one (1) sample was collected which was a background sample collected on the exterior of the building on the south side by the main entrance. The remaining sampling was rescheduled to Monday January 19th, 2015 when access to the complaint area could be secured. On January 19th, 2015 Mr. Freise again met with Mr. McCrary to collect the remaining mold samples in the complaint area. Access was gained to the complaint area at approximately 1645 hours to begin the sampling. Three (3) samples were collect which included the following: NCA1 (hallway immediately north of the complaint area), CA-1 (Treva Fields’ office), and CAw-1 (the interior wall taken from the outside southern exterior of the building). Spore Trap sampling methodology was used for this sampling event. CCRC utilized a Zefon Biopump Plus with Serial # 3014 which was pre calibrated to collect a 75 Liter sample of air from the area of investigation. The samples were collected on Air-O-Cell Spore Trap Cassettes. Spore trap
analysis includes identification of all molds present within the air sample collected and also quantification of spores per cubic meter. After collection, the spore trap samples were placed into a plastic baggy. Tape lift sampling methodology was also utilized in this sampling event. Tape lift samples collect a sample of spores from a solid surface. Tape lift samples include identification of mold spores that may be growing on solid surfaces. All mold samples were sent to QuanTEM Laboratories in Oklahoma City for analysis. The six (6) samples were collected at the following times: Sample ID
Collection Date
Collection Time
Sample Type
TL-01 TL-02 BG-1 NCA-1 CA-1 CAw-1
12/19/14 12/19/14 01/16/15 01/19/15 01/19/15 01/19/15
0945 hrs 0945 hrs 1700 hrs 1645 hrs 1655 hrs 1715 hrs
Tape Lift Tape Lift Spore Trap Spore Trap Spore Trap Spore Trap
Samples were sent to QuanTEM Laboratories and were received on January 22nd, 2015(See appendix A for Chain of Custody and Lab Report). In addition to the spore trap sampling, CCRC staff also collected ambient air conditions within the area of investigation. The ambient air conditions were collected using a Fluke 971 meter. This meter will display the relative humidity in percent, air temperature in °F, and wet bulb dew point in °F. The ambient air conditions recording within the areas of investigation are below: Sample ID CA-1
Air Temp (°F) 79.6
Relative Humidity (%) 26.1
Dew Point (°F) 58.9
The asbestos samples were analyzed on December 23rd, 2014 by Earth Tech Inc. using polarized light microscopy using the McCrone method of dispersion staining analysis (See appendix B for Lab Report). Sample Analysis Summary The mold samples were analyzed by QuanTem Laboratories in Oklahoma City, OK. The samples were analyzed using Spore Trap, Quantitative NonCulturable, MM001 methodology and Tape Lift, Qualitative NonCulturable, MM002 methodoly. QuanTem Laboratories is an American Industrial Hygiene Association (AIHA) accredited laboratory. Their AIHA ID number is 101352 The results of the mold sampling at the Tahlequah warehouse indicate a substantial mold concentration located in the complaint area (Treva Fields’ office). The air sampled contained an 80% coverage which indicates that the sampling cassette was overloaded with spores. As a result, accurate quantification of the species of mold present was not possible. The overloaded cassette does indicate that there is a significant concentration of mold spores present in the complaint area. The sample collected in the non-complaint area (NCA-1) contained a small concentration of mold spores that is significantly less the background sample (BG-1 collected outside). Sample CAw-1, collected within the southernmost exterior wall, contained mold spores at a concentration slightly larger than the background sample. (See Table 1 for a list of all detections)
Table 1.0 List of Detections (Results-Counts/m3) Sample ID CA-1* NCA-1 BG-1 CAW-1 Present 27 27 Alternaria Present 27 147 213 Ascospores 93 267 213 Aspergillus/Penicillium Group Present 67 27 Basidiospores Bipolaris/Drechslera Group Present (2) Present 67 253 680 Cladosporium Present 27 80 13 Epicoccum Present 53 160 213 Hyphal Fragments Present 13 53 120 Periconia/Myxomycetes/Smuts Present Pithomyces/Ulocladium 53 Pollen 13 13 Rusts 293 1080 1559 Total Counts 80 10 3 5 Percent Coverage % *Sample CA-1 sample cassette was overloaded quantification is not possible
TL-01
TL-02
Few Few
Few
Few Few Few Few
Few
The results of the asbestos sampling are as follows in Table 2.0: Table 2.0 List of Asbestos Detections (% fibers) Sample ID
Color
Asbestos Content
001-Wallboard 002-Baseboard & Mastic 003-Carpet Mastic
Brown Brown/Tan
None Detected None Detected
Tan
None Detected
004-Attic Insulation 005-Wall Insulation, Vermiculite
Brown Brown/Black
None Detected None Detected
Non Asbestos Content >95% Cellulose >95% Non-Fibrous Material >95% Non-Fibrous Material 100% Cellulose 3% Cellulose 97% Non-Fibrous Material
(See appendix B for Lab Report) Recommendations Cherokee CRC staff visited the site and is familiar with the conditions present within the area-ofinvestigation. The primary area of concern was identified to be the complaint area located in Treva Fields’ office in the CNE Corporate Accounting office near Tahlequah, OK. The sample collected within the complaint area (CA-1) was overloaded with mold spores. Due to these results being much larger than the background sample, it would indicate active mold colonies are present in this area (and in a much lesser case in the non-complaint area). Upon inspection of the complaint area, it was immediately apparent that there is a moisture issue present due to water infiltrating the southern interior wall and floor area around the wall. Water staining was visible on the southern interior brick wall and floor area. The floor around the base of the wall shows signs
of water damage. At the time of sampling, the humidity in the room was at a normal range of 26.1%. No visible mold growth was present within the office. Additional destructive testing is necessary to identify the source of water infiltration and mold growth. Since the mold results from within the complaint area could not be quantified, the molds of most concern cannot be determined. A list of mold species’ general characteristics is attached as appendix C. (See appendix C for a list of characteristics of the identified mold Genera)
Appendix A
Microbiology Analytical Report (Results) QuanTEM Lab ID:
245731
Date Received:
01/21/2015
Received By:
Patrick Mlekush
Analyzed By:
Morgan Lane
Date Analyzed:
01/22/2015
Methodology:
Tape, Qualitative NonCulturable, MM002
AIHA ID Number:
101352
QuanTEM Sample ID
245731-001
245731-002
TL-01
TL-02
Results
Results
Ascospores
Few
Few
Aspergillus/Penicillium Group
Few
Bipolaris/Drechslera Group (2)
Few
Cladosporium
Few
Client Sample ID
Client:
Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116
Account Number:
B535
Project:
CNE Corporate Accounting
Location:
Hwy 51, Talequah
Project No:
964
Few
Epicoccum Hyphal Fragments
Few
Few
Periconia/Myxomycetes/Smuts
Few
Few
Comments
01/23/2015
Page 1 of 2
Microbiology Analytical Report (Signature Page) QuanTEM Lab ID:
245731
Date Received:
01/21/2015
Received By:
Patrick Mlekush
Analyzed By:
Morgan Lane
Date Analyzed: Methodology: AIHA ID Number:
Client:
Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116
01/22/2015
Account Number:
B535
Tape, Qualitative NonCulturable, MM002
Project:
CNE Corporate Accounting
Location:
Hwy 51, Talequah
Project No:
964
101352
Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis. (2)Also includes spores from Exosporium, Exserohilum and Helminthosporium Few=10 or fewer fungal structures detected over area analyzed; Abundant=fungal structures detected in 75% or more of the area analyzed or more than 500 fungal structures present; Moderate=fungal structure concentrations between few & abundant. The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is the responsibility of the company or individual who conducted the investigation. This report shall not be reproduced except in full, without the written approval of the laboratory. This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government
Approved: Morgan Lane, Analyst
01/23/2015
Page 2 of 2
Microbiology Analytical Report (Results) QuanTEM Lab ID:
245731
Date Received:
01/21/2015
Received By:
Patrick Mlekush
Analyzed By:
Morgan Lane
Date Analyzed: Methodology: AIHA ID Number:
Client:
Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116
01/22/2015
Account Number:
B535
Spore Trap, Quantitative NonCulturable, MM001
Project:
CNE Corporate Accounting
Location:
Hwy 51, Talequah
101352
Project No:
964
QuanTEM Sample ID
245731-003
245731-004
245731-005
245731-006
CA-1
CAw-1
NCA-1
BG-1
Volume (L)
75
75
75
75
Detection Limit
13
13
13
13
Results Counts/m³ 27
Results Counts/m³
Alternaria
Results Counts/m³ Present
Results Counts/m³ 27
Ascospores
Present
213
27
147
213
93
267
Client Sample ID
Aspergillus/Penicillium Group Basidiospores
Present
Bipolaris/Drechslera Group (2)
Present
Cladosporium
Present
Epicoccum Hyphal Fragments
27
67
680
67
Present
13
27
80
Present
213
53
160 13
Nigrospora Periconia/Myxomycetes/Smuts
Present
Pithomyces/Ulocladium
Present
Pollen
120
13
53
13
13
1559
293
1080
5
10
3
53
Rusts Overloaded; Mold Present
X
Total Results (Counts/m³) Percent Coverage (%) Comments
01/23/2015
253
80 Overloaded; Mold Present
Page 1 of 3
Microbiology Analytical Report (Counts) QuanTEM Lab ID:
245731
Date Received:
01/21/2015
Received By:
Patrick Mlekush
Analyzed By:
Morgan Lane
Date Analyzed: Methodology: AIHA ID Number:
Client:
Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116
01/22/2015
Account Number:
B535
Spore Trap, Quantitative NonCulturable, MM001
Project:
CNE Corporate Accounting
Location:
Hwy 51, Talequah
101352
Project No:
964
QuanTEM Sample ID Client Sample ID Volume (L)
245731-003
245731-004
245731-005
245731-006
CA-1
CAw-1
NCA-1
BG-1
75
75
75
75
Counts
Counts
Counts
Counts
Alternaria
2
Ascospores
16
2
11
2
Aspergillus/Penicillium Group
16
7
20
Basidiospores
2
Cladosporium
51
5
Epicoccum
1
2
6
Hyphal Fragments
16
4
12
Periconia/Myxomycetes/Smuts
9
1
4
Pollen
4 1
1
5
1
Nigrospora
Rusts
01/23/2015
19
Page 2 of 3
Microbiology Analytical Report (Signature Page) QuanTEM Lab ID:
245731
Date Received:
01/21/2015
Received By:
Patrick Mlekush
Analyzed By:
Morgan Lane
Date Analyzed: Methodology: AIHA ID Number:
Client:
Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116
01/22/2015
Account Number:
B535
Spore Trap, Quantitative NonCulturable, MM001
Project:
CNE Corporate Accounting
Location:
Hwy 51, Talequah
101352
Project No:
964
Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis. (2)Also includes spores from Exosporium, Exserohilum and Helminthosporium Percent coverage = amount of particulate matter. With 25-50% coverage, results may be underestimated; with 50-70% coverage, results will be underestimated; with >70% coverage, slides are designated overloaded (too dirty to count). The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is the responsibility of the company or individual who conducted the investigation. This report shall not be reproduced except in full, without the written approval of the laboratory. This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government.
Approved: Morgan Lane, Analyst
01/23/2015
Page 3 of 3
Appendix B
12/23/14 Cherokee CRC John Freise 10838 E. Marshall St., Suite 220 Tulsa, OK 74116
Project Name: CWE Corporate Accounting Office
Sample No.
Color
Asbestos Non Asbestos Content Content ________________________________________________________________________________ 001 Brown None Detected >95% Cellulose TR Office – Wallboard 002 Brown/Tan TR Office – Baseboard & Mastic
None Detected
>95% Non Fibrous Material
003 Tan TR Office Carpet Mastic
None Detected
>95% Non Fibrous Material
004 Brown Attic – Brown Insulation
None Detected
100% Cellulose
005 Brown South Wall – Wall Insulation Vermiculite, Black
None Detected
3% Cellulose 97% Non Fibrous Material
I certify that the above samples were analyzed by Polarized Light Microscopy using the McCrone method of dispersion staining analysis.
Earth Tech Enterprises, Inc. 3336 E. 32nd Street, Suite 234 Tulsa, OK 74135 918-712-9163
Appendix C