MOLD SAMPLING ANALYSIS REPORT

Cherokee CRC, LLC 10838 E Marshall Street Suite 220 Tulsa, Oklahoma 74116 MOLD SAMPLING ANALYSIS REPORT For Cherokee Nation Entertainment Corporate...
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Cherokee CRC, LLC 10838 E Marshall Street Suite 220 Tulsa, Oklahoma 74116

MOLD SAMPLING ANALYSIS REPORT

For

Cherokee Nation Entertainment Corporate Accounting Office Tahlequah, OK

Introduction Cherokee CRC, LLC, conducted a mold and asbestos sampling investigation in the Cherokee Nation Entertainment Corporate accounting office located on Hwy 51 near Tahlequah, OK. The sampling was conducted on December 19th, 2014, January 16th, 2015, and January 19th, 2015. Work was performed at the request of Joe Washum, Director of Safety and Environmental for Cherokee Nation Businesses and Kevin Ogle, Director of Construction for Cherokee Nation Businesses. The purpose of the sampling was to determine if any unacceptable concentrations of mold spores exist in the sampled locations that could be negatively impacting employee health and to determine if any materials in the complaint area contained asbestos containing materials. Cherokee CRC, LLC coordinated with Mr. Washum and Matthew McCrary (CNE Safety and Environmental) to conduct the sampling and to access the areas of investigation. The sampling was conducted in a manner to minimize any disturbance of the mold colonies present inside the building while providing maximum safety for project personnel and employees present during the sampling.

Sampling Methodology The initial sampling took place on December 19th at the facility during a walkthrough with Kevin Ogle and Joe Washum. The samples collected at this time included possible asbestos containing materials within the building, more specifically, in the primary complaint area identified as Treva Fields’ office. Five (5) samples were collected from suspect materials that could possibly contain asbestos fibers. These samples included the complaint office’s wallboard around the window sample 001, the baseboard and mastic sample 002, the carpet mastic 003, the brownish yellow attic insulation present throughout the building, and a sample of the vermiculite from the wall cavity. Two (2) tape lift mold samples were collected from the complaint area (Treva Fields’ Office) on December 19th. These samples included suspect white residue on the brick inner wall, and a rusty red spot on the carpet at the base of the southern interior wall. Additional mold sampling occurred on January 16th, 2015. John Freise met with Mr. McCrary who arranged access to the building for after-hours sampling as not to disturb employees who working in the building. However, access was not available to the complaint area of the building on the 16th so only one (1) sample was collected which was a background sample collected on the exterior of the building on the south side by the main entrance. The remaining sampling was rescheduled to Monday January 19th, 2015 when access to the complaint area could be secured. On January 19th, 2015 Mr. Freise again met with Mr. McCrary to collect the remaining mold samples in the complaint area. Access was gained to the complaint area at approximately 1645 hours to begin the sampling. Three (3) samples were collect which included the following: NCA1 (hallway immediately north of the complaint area), CA-1 (Treva Fields’ office), and CAw-1 (the interior wall taken from the outside southern exterior of the building). Spore Trap sampling methodology was used for this sampling event. CCRC utilized a Zefon Biopump Plus with Serial # 3014 which was pre calibrated to collect a 75 Liter sample of air from the area of investigation. The samples were collected on Air-O-Cell Spore Trap Cassettes. Spore trap

analysis includes identification of all molds present within the air sample collected and also quantification of spores per cubic meter. After collection, the spore trap samples were placed into a plastic baggy. Tape lift sampling methodology was also utilized in this sampling event. Tape lift samples collect a sample of spores from a solid surface. Tape lift samples include identification of mold spores that may be growing on solid surfaces. All mold samples were sent to QuanTEM Laboratories in Oklahoma City for analysis. The six (6) samples were collected at the following times: Sample ID

Collection Date

Collection Time

Sample Type

TL-01 TL-02 BG-1 NCA-1 CA-1 CAw-1

12/19/14 12/19/14 01/16/15 01/19/15 01/19/15 01/19/15

0945 hrs 0945 hrs 1700 hrs 1645 hrs 1655 hrs 1715 hrs

Tape Lift Tape Lift Spore Trap Spore Trap Spore Trap Spore Trap

Samples were sent to QuanTEM Laboratories and were received on January 22nd, 2015(See appendix A for Chain of Custody and Lab Report). In addition to the spore trap sampling, CCRC staff also collected ambient air conditions within the area of investigation. The ambient air conditions were collected using a Fluke 971 meter. This meter will display the relative humidity in percent, air temperature in °F, and wet bulb dew point in °F. The ambient air conditions recording within the areas of investigation are below: Sample ID CA-1

Air Temp (°F) 79.6

Relative Humidity (%) 26.1

Dew Point (°F) 58.9

The asbestos samples were analyzed on December 23rd, 2014 by Earth Tech Inc. using polarized light microscopy using the McCrone method of dispersion staining analysis (See appendix B for Lab Report). Sample Analysis Summary The mold samples were analyzed by QuanTem Laboratories in Oklahoma City, OK. The samples were analyzed using Spore Trap, Quantitative NonCulturable, MM001 methodology and Tape Lift, Qualitative NonCulturable, MM002 methodoly. QuanTem Laboratories is an American Industrial Hygiene Association (AIHA) accredited laboratory. Their AIHA ID number is 101352 The results of the mold sampling at the Tahlequah warehouse indicate a substantial mold concentration located in the complaint area (Treva Fields’ office). The air sampled contained an 80% coverage which indicates that the sampling cassette was overloaded with spores. As a result, accurate quantification of the species of mold present was not possible. The overloaded cassette does indicate that there is a significant concentration of mold spores present in the complaint area. The sample collected in the non-complaint area (NCA-1) contained a small concentration of mold spores that is significantly less the background sample (BG-1 collected outside). Sample CAw-1, collected within the southernmost exterior wall, contained mold spores at a concentration slightly larger than the background sample. (See Table 1 for a list of all detections)

Table 1.0 List of Detections (Results-Counts/m3) Sample ID  CA-1* NCA-1 BG-1 CAW-1 Present 27 27 Alternaria Present 27 147 213 Ascospores 93 267 213 Aspergillus/Penicillium Group Present 67 27 Basidiospores Bipolaris/Drechslera Group Present (2) Present 67 253 680 Cladosporium Present 27 80 13 Epicoccum Present 53 160 213 Hyphal Fragments Present 13 53 120 Periconia/Myxomycetes/Smuts Present Pithomyces/Ulocladium 53 Pollen 13 13 Rusts 293 1080 1559 Total Counts 80 10 3 5 Percent Coverage % *Sample CA-1 sample cassette was overloaded quantification is not possible

TL-01

TL-02

Few Few

Few

Few Few Few Few

Few

The results of the asbestos sampling are as follows in Table 2.0: Table 2.0 List of Asbestos Detections (% fibers) Sample ID

Color

Asbestos Content

001-Wallboard 002-Baseboard & Mastic 003-Carpet Mastic

Brown Brown/Tan

None Detected None Detected

Tan

None Detected

004-Attic Insulation 005-Wall Insulation, Vermiculite

Brown Brown/Black

None Detected None Detected

Non Asbestos Content >95% Cellulose >95% Non-Fibrous Material >95% Non-Fibrous Material 100% Cellulose 3% Cellulose 97% Non-Fibrous Material

(See appendix B for Lab Report) Recommendations Cherokee CRC staff visited the site and is familiar with the conditions present within the area-ofinvestigation. The primary area of concern was identified to be the complaint area located in Treva Fields’ office in the CNE Corporate Accounting office near Tahlequah, OK. The sample collected within the complaint area (CA-1) was overloaded with mold spores. Due to these results being much larger than the background sample, it would indicate active mold colonies are present in this area (and in a much lesser case in the non-complaint area). Upon inspection of the complaint area, it was immediately apparent that there is a moisture issue present due to water infiltrating the southern interior wall and floor area around the wall. Water staining was visible on the southern interior brick wall and floor area. The floor around the base of the wall shows signs

of water damage. At the time of sampling, the humidity in the room was at a normal range of 26.1%. No visible mold growth was present within the office. Additional destructive testing is necessary to identify the source of water infiltration and mold growth. Since the mold results from within the complaint area could not be quantified, the molds of most concern cannot be determined. A list of mold species’ general characteristics is attached as appendix C. (See appendix C for a list of characteristics of the identified mold Genera)

Appendix A

Microbiology Analytical Report (Results) QuanTEM Lab ID:

245731

Date Received:

01/21/2015

Received By:

Patrick Mlekush

Analyzed By:

Morgan Lane

Date Analyzed:

01/22/2015

Methodology:

Tape, Qualitative NonCulturable, MM002

AIHA ID Number:

101352

QuanTEM Sample ID

245731-001

245731-002

TL-01

TL-02

Results

Results

Ascospores

Few

Few

Aspergillus/Penicillium Group

Few

Bipolaris/Drechslera Group (2)

Few

Cladosporium

Few

Client Sample ID

Client:

Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116

Account Number:

B535

Project:

CNE Corporate Accounting

Location:

Hwy 51, Talequah

Project No:

964

Few

Epicoccum Hyphal Fragments

Few

Few

Periconia/Myxomycetes/Smuts

Few

Few

Comments

01/23/2015

Page 1 of 2

Microbiology Analytical Report (Signature Page) QuanTEM Lab ID:

245731

Date Received:

01/21/2015

Received By:

Patrick Mlekush

Analyzed By:

Morgan Lane

Date Analyzed: Methodology: AIHA ID Number:

Client:

Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116

01/22/2015

Account Number:

B535

Tape, Qualitative NonCulturable, MM002

Project:

CNE Corporate Accounting

Location:

Hwy 51, Talequah

Project No:

964

101352

Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis. (2)Also includes spores from Exosporium, Exserohilum and Helminthosporium Few=10 or fewer fungal structures detected over area analyzed; Abundant=fungal structures detected in 75% or more of the area analyzed or more than 500 fungal structures present; Moderate=fungal structure concentrations between few & abundant. The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is the responsibility of the company or individual who conducted the investigation. This report shall not be reproduced except in full, without the written approval of the laboratory. This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government

Approved: Morgan Lane, Analyst

01/23/2015

Page 2 of 2

Microbiology Analytical Report (Results) QuanTEM Lab ID:

245731

Date Received:

01/21/2015

Received By:

Patrick Mlekush

Analyzed By:

Morgan Lane

Date Analyzed: Methodology: AIHA ID Number:

Client:

Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116

01/22/2015

Account Number:

B535

Spore Trap, Quantitative NonCulturable, MM001

Project:

CNE Corporate Accounting

Location:

Hwy 51, Talequah

101352

Project No:

964

QuanTEM Sample ID

245731-003

245731-004

245731-005

245731-006

CA-1

CAw-1

NCA-1

BG-1

Volume (L)

75

75

75

75

Detection Limit

13

13

13

13

Results Counts/m³ 27

Results Counts/m³

Alternaria

Results Counts/m³ Present

Results Counts/m³ 27

Ascospores

Present

213

27

147

213

93

267

Client Sample ID

Aspergillus/Penicillium Group Basidiospores

Present

Bipolaris/Drechslera Group (2)

Present

Cladosporium

Present

Epicoccum Hyphal Fragments

27

67

680

67

Present

13

27

80

Present

213

53

160 13

Nigrospora Periconia/Myxomycetes/Smuts

Present

Pithomyces/Ulocladium

Present

Pollen

120

13

53

13

13

1559

293

1080

5

10

3

53

Rusts Overloaded; Mold Present

X

Total Results (Counts/m³) Percent Coverage (%) Comments

01/23/2015

253

80 Overloaded; Mold Present

Page 1 of 3

Microbiology Analytical Report (Counts) QuanTEM Lab ID:

245731

Date Received:

01/21/2015

Received By:

Patrick Mlekush

Analyzed By:

Morgan Lane

Date Analyzed: Methodology: AIHA ID Number:

Client:

Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116

01/22/2015

Account Number:

B535

Spore Trap, Quantitative NonCulturable, MM001

Project:

CNE Corporate Accounting

Location:

Hwy 51, Talequah

101352

Project No:

964

QuanTEM Sample ID Client Sample ID Volume (L)

245731-003

245731-004

245731-005

245731-006

CA-1

CAw-1

NCA-1

BG-1

75

75

75

75

Counts

Counts

Counts

Counts

Alternaria

2

Ascospores

16

2

11

2

Aspergillus/Penicillium Group

16

7

20

Basidiospores

2

Cladosporium

51

5

Epicoccum

1

2

6

Hyphal Fragments

16

4

12

Periconia/Myxomycetes/Smuts

9

1

4

Pollen

4 1

1

5

1

Nigrospora

Rusts

01/23/2015

19

Page 2 of 3

Microbiology Analytical Report (Signature Page) QuanTEM Lab ID:

245731

Date Received:

01/21/2015

Received By:

Patrick Mlekush

Analyzed By:

Morgan Lane

Date Analyzed: Methodology: AIHA ID Number:

Client:

Cherokee CRC 10838 E. Marshall St. Suite 220 Tulsa, OK 74116

01/22/2015

Account Number:

B535

Spore Trap, Quantitative NonCulturable, MM001

Project:

CNE Corporate Accounting

Location:

Hwy 51, Talequah

101352

Project No:

964

Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis. (2)Also includes spores from Exosporium, Exserohilum and Helminthosporium Percent coverage = amount of particulate matter. With 25-50% coverage, results may be underestimated; with 50-70% coverage, results will be underestimated; with >70% coverage, slides are designated overloaded (too dirty to count). The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is the responsibility of the company or individual who conducted the investigation. This report shall not be reproduced except in full, without the written approval of the laboratory. This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government.

Approved: Morgan Lane, Analyst

01/23/2015

Page 3 of 3

Appendix B

12/23/14 Cherokee CRC John Freise 10838 E. Marshall St., Suite 220 Tulsa, OK 74116

Project Name: CWE Corporate Accounting Office

Sample No.

Color

Asbestos Non Asbestos Content Content ________________________________________________________________________________ 001 Brown None Detected >95% Cellulose TR Office – Wallboard 002 Brown/Tan TR Office – Baseboard & Mastic

None Detected

>95% Non Fibrous Material

003 Tan TR Office Carpet Mastic

None Detected

>95% Non Fibrous Material

004 Brown Attic – Brown Insulation

None Detected

100% Cellulose

005 Brown South Wall – Wall Insulation Vermiculite, Black

None Detected

3% Cellulose 97% Non Fibrous Material

I certify that the above samples were analyzed by Polarized Light Microscopy using the McCrone method of dispersion staining analysis.

Earth Tech Enterprises, Inc. 3336 E. 32nd Street, Suite 234 Tulsa, OK 74135 918-712-9163

Appendix C