Maria del Pilar Castillo - CV Maria del Pilar Castillo, Researcher JTI – Swedish Institute of Agricultural and Environmental Engineering P.O. Box 7033 SE-750 07 Uppsala Sweden E-mail: [email protected] Phone: + 46-(0)10-5166987 + 46-(0)70 3901303

Key qualifications Worked with research as project manager and implementer of more than 20 years in the fields of biological decomposition of organic material for biogas production, reduction of pollution by chemical pesticides with biobeds and bioremediation of contaminated soils. I perform laboratory studies, pilot studies, feasibility studies and investigative assignments. At present responsible for the optimization of biogas processes at laboratory scale at the JTI Biogas Laboratory. Work in close collaboration with several universities and companies. Education  BSc. Chemical Engineering, 1980, University of San Marcos, Lima, Peru.  MSc. Biotechnology, 1992, Royal Institute of Technology, Stockholm, Sweden.  Technology Doctor in Microbiology, 1997, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. “Degradation of pesticides with the white rot fungus Phanerochaete chrysosporium in solid substrate fermentation”. Supervisors: Lennart Torstensson, John Stenström, Paul Ander (all from SLU).  Post-doc position at Wageningen Agricultural University, The Netherlands 1997-1998.  Associate professor, 2015-04-21. Employments  Researcher at the Institute of Technological Industrial Research and Technical Standards, ITINTEC, Lima, Peru 1979-1986.  Research assistant at the JTI - Swedish Institute of Agricultural Engineering 1987-1988 Uppsala, Sweden.  Research assistant at the Department of Microbiology, SLU 1990-1992.  PhD student at the Department of Microbiology, SLU, Uppsala, Sweden 1992-1997.  Researcher at the Department of Microbiology, SLU, Uppsala, Sweden 1997-2008.  Researcher at JTI- Institute for Agricultural and Environmental Engineering 2008 – at present. 100% for research. Special commissions, assigments  One of the pioneers of the technology of biobeds for minimizing pesticide contamination and international diffusion of biobeds in the world.  Referee for: Journal Environmental Science & Technology, FEMS Microbiology Ecology, Biotechnology & Bioengineering, Chemosphere, Pest Management Science,

 





   

 

Biodegradation, FEMS Microbiology Letters, Journal of Applied Microbiology, International Journal of Environmental Analytical Chemistry External examiner for the doctoral thesis of Paul Fogg, Cranfield University, United Kingdom, April 27, 2004. “Fate and behaviour of pesticides in biobeds”. Opponent at the doctoral thesis dissertation of Enrique Terrazas Siles, Lund University, Sweden, December 15, 2005. “Fungal redox enzymes involved in oxidation of organic pollutants”. Member of the Examination Board for the doctoral thesis of Olga Margarita Rubilar Araneda, Universidad de la Frontera, Temuco, Chile, October 29, 2007. “Degradación de pentaclorofenol en medio líquido y suelo alofánico por Anthracophyllum discolour”. External reviewer for proposals submitted to the 2008-2011 Regular Research Funding Competition, National Fund for Scientific and Technological Development (FONDECYT), Chilean Government Commission for Scientific and Technological Development (CONICYT). External reviewer for proposals submitted to the 2012 Research Funding Competition, Agencia Nacional de Investigación e Innovación, Uruguay. Teacher at the Biogas process course held at SLU and organized by Avfall Sverige, Uppsala, October 22-23, 2012. Webmanager, together with Jens Husby, Bayer CropScience, of the webpage Biobeds.org. Support in the preparation of Stewardship material for Bayer Nordic, biobeds video. http://www.nordic.bayer.com/en/sustainability/sustainable-development/productstewardship---biobeds). International research advisor several projects at the Universidad de La Frontera, Temuco, Chile Member of the organization commitees for organization of the European and Iberoamerican Biobed Workshops. Malmö, Sweden 2004; Gent, Belgium 2007; Piacenza, Italy 2010; Temuco, Chile 2012; Wageningen, The Netherlands 2013; Guatemala 2014.

List of publications Some recent refereed publications  Pizzul, L., Castillo, M.d.P. and Stenström, J. (2007) Effect of rapeseed oil on the degradation of PAH in soil by Rhodococcus wratislaviensis International Biodeterioration & Biodegradation 59, (2), 111-118  Pizzul, L., Sjögren, Å, Castillo M.d.P. and Stenström, J. (2007) Degradation of polycyclic aromatic hydrocarbons in soil by a two-step sequential treatment. Biodegradation 18, 607-616.  Coppola, L., Castillo, M.d.P., Monaci, E., and Vischetti, C. (2007) Adaptation of the biobed composition for chlorpyrifos degradation to Southern Europe conditions. Journal of Agricultural and Food Chemistry. 55 (2):396-401.

 Vischetti, C.; Coppola, L.; Monaci, E.; Cardinali, A.; and Castillo, M.d.P. (2007) Microbial impact of the pesticide chlorpyrifos in Swedish and Italian biobeds. Agronomy for Sustainable Development. 27 (3) 267-272.  Castillo, M. d. P.; Torstensson, L. (2007) Effect of biobed composition, moisture and temperature on the degradation of pesticides. Journal of Agricultural and Food Chemistry 55(14) 5725-5733.  Castillo, M. d. P., Torstensson, L. and Stenstrom, J. (2008) Biobeds for environmental protection from pesticide use - A review. Journal of Agricultural and Food Chemistry 56: 6206-6219.  Pizzul, L., M. D. Castillo, et al. (2009). "Degradation of glyphosate and other pesticides by ligninolytic enzymes." Biodegradation 20(6): 751-759.  Hultgren, J.; Pizzul, L.; Castillo, M.d.P. and Granhall. U. (2010) Degradation of PAH in a creosote-contaminated soil. A comparison between the effects of willows (Salix viminalis), wheat straw and a nonionic surfactant. International Journal of Phytoremediation 12, 1 54 – 66.  Acevedo, F., Pizzul, L.,Castillo, M. d.P., Gonzalez, M.E., Cea, M., Gianfreda, L. and Diez, M.C. (2010). Degradation of polycyclic aromatic hydrocarbons by free and nanoclayimmobilized manganese peroxidase from Anthracophyllum discolor. Chemosphere 80(3): 271-278.  Coppola, L., MdP Castillo, et al (2011). “Degradation of isoproturon and bentazone in peat- and compost-based biomixtures” Pest Management Science 67, 107-113.  Acevedo, F., L. Pizzul, et al. (2011). "A Practical Culture Technique for Enhanced Production of Manganese Peroxidase by Anthracophyllum discolor Sp4." Brazilian Archives of Biology and Technology 54(6): 1175-1186.  Tortella, G. R., O. Rubilar, et al. (2012). "Chlorpyrifos degradation in a biomixture of biobed at different maturity stages." Chemosphere 88(2): 224-228.  Teghammar, A., Castillo, M.d.P., Ascue, J., Niklasson, C., Horvath, I.S. (2013). Improved anaerobic digestion by the addition of paper tube residuals; pretreatment, stabilizing and synergetic effects. Energy & Fuels Vol. 27. Some of latest research projects and reports  Waste Refinery, SP Technical Research Institute of Sweden.(2010). Pretreatment of lignocellulosic material for enhanced biogas production. Sarvari-Horvath, I. (University of Borås), Castillo, M.d.P. (JTI), Nordman, R (SP).  Waste Refinery 33. (2011) Environmental effects of the use of polymers in the biogas industry – Pre study. Castillo, M.d.P., Henriksson, G. (SP), Jakubowicz, J. (SP).  Värmeforsk Project SYS08-853. (2012) High-temperature pretreatment of biogas substrate by using district heating to increase the biogas production. Castillo, M.d.P.(JTI), Olsson, M. (SP), Henriksson, G. (SP), Nordman, R. (SP).  Waste Refinery 45. (2013) Biogas from lignocellulosic biomass A techno-economic study of pretreatment with NMMO. Sárvári-Horváth, I. (SP), Castillo, M.d.P. (JTI), Laurell J. (JTI).  Swedish Agency for Economic and Regional Growth – Tillväxtverket (2013). Application of biobed techniques in China to reduce pesticide pollution in agricultural filed and water system. Castillo, M.d.P., Feng, X.  CKB-SLU. Inventory of Biobeds in Sweden 2013. Castillo, M.d.P. (JTI), Nilsson, E. (VISAVI).

   

SGC-Svensk Gas Center (2013). Fungal pretreatment of straw for enhanced biogas yield. Castillo, M.d.P., Feng, X., Schnürer, A. Waste Refinery 61 (2013). Strategies for improve anaerobic digestion of substrates with high content of lignocellulose and nitrogen. Castillo, M.d.P., Edström, M. (JTI). Waste Refinery 62 (2013). Increased methane production by new substrates and process development. Murto, M. (Anoxkaldnes), Castillo, M.d.P. (JTI), Lagerkvist, A. (Luleå Technical University), Sárvári-Horváth, I. (University of Borås). Björnsson, L.; Castillo, M.d.P; Gunnarsson, C.; Svensson, S-E.; Wallberg, O. (2014). Förbehandling av lignocellulosarika råvaror vid biogasproduktion – Nyckelaspekter vid jämförande utvärdering. Swedish Energy Agency, University of Lund.

Chapters in books 

M.d.P. Castillo, L. Torstensson (2008) Biobeds - Biotechnology for Environmental Protection from Pesticide Pollution; in Methods and Techniques for Cleaning-up Contaminated Sites. Proceedings of the NATO Advanced Research Workshop on Methods and Techniques for Cleaning-up Contaminated Sites, Sinaia, Romania, 9-11 October 2006. Annable, M.D.; Teodorescu, M.; Hlavinek, P.; Diels, L. (Eds.). Series: NATO Science for Peace and Security Series. Subseries: NATO Science for Peace and Security Series C: Environmental Security. ISBN: 978-1-4020-6874-4

Recent oral presentations  Castillo, M.d.P. and Torstensson, L. Biobeds – biotechnology for environmental protection from pesticide use. Lecture at the Universidad Mayor, Santiago, Chile, 2007.  Castillo, M.d.P., Stenström, J. and Torstensson, L. Biobeds for environmental protection from pesticide use - A review. Oral presentation at the conference “Pesticide Behaviour in Soils, Water and Air”. York, UK. 14 – 16 September, 2009.  Castillo, M.d.P. Tecnología de lechos biológicos para la remoción de plaguicidas. Oral presentation at the Seminar Hacia una agricultura sustenstable- Tecnología y Medio Ambiente. Universidad de La Frontera, Centro de Biotecnología Ambiental, Instituto de Agroindustria, Temuco, Chile, 17 November 2009.  Castillo, M.d.P. Management of organic residues in Sweden. Oral presentation at the Seminar Biomass and Energy – Biogas. Biomasa Araucanía, CORFO-Chile, CIDGRO-Chile Temuco, Chile, 8 June November 2011.  Castillo, M.d.P. Reducción de la contaminación por plaguicidas en el sector frutícola: Tecnología de las biobeds. Universidad de La Frontera, Centro de Biotecnología Ambiental, Instituto de Agroindustria, Proyecto FONDEF, Temuco, Chile, 16 November 2011.  Castillo, M.d.P. Biobeds in the world- Summary and strategies. Organizer and oral presentation. First Latin-American Workshop on Biobeds, Pucón, Chile. 2 – 4 May 2012.  Castillo, M.d.P. Biobeds – past, presenta and future. Organizer and oral presentation. Second Latin-American Workshop on Biobeds, Guatemala city, Guatemala. June 2014.

Popular publications and videos  BIOBEDS – A SWEDISH CONTRIBUTION TO ENVIRONMENTAL PROTECTION FROM PESTICIDE POLLUTION http://www.slu.se/Documents/externwebben/nlfak/mikrobiologi/Biob%c3%a4ddar/Biobeds%202011.pdf  BIOBÄDDAR – ETT SVENSKT BIDRAG TILL SKYDD FÖR MILJÖN FRÅN FÖRORENING MED BEKÄMPNINGSMEDEL http://www.slu.se/Documents/externwebben/nlfak/mikrobiologi/Biob%c3%a4ddar/Biob%c3%a4ddar%202011.pdf  CAMAS BIOLÓGICAS – BIOTECNOLOGÍA PARA LA PRESERVACIÓN DEL MEDIO AMBIENTE DE LA CONTAMINACIÓN POR PESTICIDAS http://www.slu.se/Documents/externwebben/nlfak/mikrobiologi/Biob%c3%a4ddar/Camas%20biol%c3%b3gicas%202011.pdf  AgroTV Guatemala (2014) (https://www.youtube.com/watch?v=93LWpUK7aRo&feature=youtu.be)