LSM 510 Laser Scanning Microscope

LSMBR_GB.qxp 26.05.1998 17:41 Uhr Seite 3 LSM 510 Laser Scanning Microscope Confocal Compact Versatile. LSMBR_GB.qxp 26.05.1998 17:41 Uhr Se...
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LSMBR_GB.qxp

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LSM 510 Laser Scanning Microscope

Confocal Compact Versatile.

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LSM 510 – flexible... The confocal LSM 510 Laser Scanning Microscope puts you in command of advanced, flexible technology. The compact scanning module can be configured in multiple ways to match a wide variety of specimens and applications.

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Legends to illustrations see last page.

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LSM 510 Laser Scanning Microscope – The Synthesis of Experience The LSM 510 from the pioneer of laser scanning microscopy. The natural result of 150 years of innovation in optics and more than 12 years of experience in all relevant fields of laser scanning microscopy. The LSM 510 is the powerful combination of confocal microscopy and the fully automated research microscopes Axioplan 2 and Axiovert – everything from Carl Zeiss.

The confocal beam path principle

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Confocal – compact – versatile

Brilliant, laser-sharp images

Superbly engineered, here is system integration at its best: Transferring the compact scanning module from one microscope to another takes only a few minutes. The researcher can use the LSM 510 on an inverted microscope in the morning and on an upright model in the afternoon, and achieve excellent results with both. To ensure this, it takes more than a few pinholes. The quality and resolving power of confocal imaging depend on many factors. We have designed a new optical train and recomputed every detail, from the microscope to the ICS objectives to the scanner and pinhole optics, to ensure optimized performance.

The LSM 510 resolves no less than 2048 x 2048 pixels – more than four million! Together with scanning fields of unbeatable size, this allows you to image even large overview pictures without losing any information. Four independent 12-bit analog-to-digital converters providing up to 4096 brightness levels give you an ample range of control and variation to optimize a wide range of images.

The freedom you need To investigate your multi-fluorescence specimens, the LSM 510 offers four simultaneous confocal channels for reflection and fluorescence, plus an extra channel for transmitted-light examination. Each of the four channels can be separately controlled, with all degrees of freedom for pinhole adjustment. Alternatively, you may have the computer optimize everything for your specific application. This individual freedom provides the flexibility you need for your research. The LSM 510 lets you effortlessly handle multi-labeling images with extremely differing brightnesses, and reproduce them at any time with a mere mouse click.

Human synovial cells, 4 confocal fluorescence channels, nuclear structures (DAPI, blue), microtubuli (CY5, red), actin filaments (FITC, green), nucleoli (Texas Red, white) (Dr. Albini, Boston)

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nce and Innovation.

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Modular flexibility Mounted directly below the inverted Axiovert 100 M BP (base port) microscope stand, the scanning module does not impede your free access to the microscope. Moreover, it ensures maximum light yield for your photon-hungry applications. On the Axiovert 100 M SP (side port), the scanning module fits to a lateral port. This facilitates fast changeover if you want to use the same scanning module on the upright Axioplan 2 as well.

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Controlled complexity

Simplicity at two levels

The LSM 510 comes with tailor-made software for full system control. With all controls motorized, fast method changes and automatic operation are exceptionally easy. To reproduce results at any time, day after day, all you need to do is to press a button. In multi-user operation, every user can save and reload their own setting parameters or complex examination procedures and thus have their own individual research tool in one and the same setup, without disturbing other user settings.

Confocal microscopy has its own laws. Whether you are an expert or a novice, or working in multiple use environments, it's no problem: the LSM 510 will give you expert results. This is because the software offers you two levels of operation. The easy-to-use operator interface guides you to the correct results with a minimum of instructions and graphical user prompting with automatic setting of many parameters for day-to-day routine. Simply follow the system's recommendations, or overrule them manually as your specimens require. The expert level below the operator interface provides in-depth control – the perfect tool for individual settings of functions and parameters for the more advanced user.

Impressive package of advanced features Setting the desired laser line and the necessary brightness on the LSM 510 is performed within microseconds, and without any moving parts. The result is monitored by a reference photodiode, which you can use as a sixth channel. This is ideal for morphology and physiology, for experiments such as ratio imaging, release of caged compounds, or FRAP. Digital control enables entirely new, sophisticated scanning techniques. The available spectral range starts with UV, for work in the life sciences, and ends with IR, for multi-photon microscopy. Other features include automatic Z aberration adjustment for all colors by collimator optics, top-grade photomultipliers for fluorescence and transmitted light, external channels and many more, which add up to perfection down to the last detail.

HRZ 400 Motorized Galvanometric Z-Stage

Flexible presentation of images

Graphical user prompting

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... with no compromise Forget about “ORs” in laser scanning microscopy. From now on, it's only “AND”. Modular AND confocal AND upgradeable AND inverted AND upright. High-tech sophistication AND flexibility. Compact design providing stability and shortest light paths, optical precision, innovative technology and ingenious scanning techniques combine to produce perfect 3D images of all specimen structures.

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LSM 510 Laser Scanning Microscope Specification Microscopes

Z motor HRZ-200

XY scanning stage

Scanning module

Inverted Axiovert 100 M BP or SP Upright Axioplan 2 MOT Fixed stage Axioskop 2 FS MOT All accessories (ICS objectives, filters etc.) can be used. DC servomotor, optoelectronic coding, stepping size 50 nm Galvanometric stage of extra-high precision, focusing range 200 µm, step size 7 nm, accuracy 40 nm, permitting continuous Z scan with up to 10 Hz Motorized, range 100 mm x 100 mm, smallest step size 0.25 µm 2 independently operating galvanometric mirrors

Resolution Zoom Field size

up to 2048 x 2048 pixels 1x ... 8x, infinitely variable up to 18 mm (1x zoom, Plan-Neofluar 1.25x/0.04 objective) Detectors up to 4 channels simultaneously, 4 PMTs for R/FL, 1 PMT for transmitted light, monitor diode Pinholes 1 variable pinhole each for up to 4 confocal channels Dynamic range 12 bit A/D converter for each detection channel

VIS laser module

Visible light fiber coupling, single mode, polarization preserving Laser beam attenuation for all visible light lasers by a single temperature stabilized AOTF HeNe laser (543 nm, 1 mW) HeNe laser (633 nm, 5 mW) Ar laser (458, 488, 514 nm, 25 mW) Ar laser (488 nm, 15 mW) ArKr laser (488 nm, 568 nm, 30 mW)

UV laser module

Single mode, polarization preserving laser beam attenuation for all lasers by UV-AOTF, temperature stabilized KrAr laser (351 nm, 364 nm, 80 mW)

Electronics module Computer

Control circuitry for microscope, scanning and laser modules

Software

Windows NT operating system: LSM software for controlling the microscope, scanning and laser modules, image recording, and the analysis, presentation and management of image data

High-end INTEL® computer, ample RAM space, extra-large hard disk, high-resolution true-color graphics card, largescreen monitor, keyboard, mouse

Development of the LSM 510 in cooperation with EMBL Heidelberg Internet: www.embl-heidelberg.de Legends to the illustration on page 7

Legends to the illustration on page 2 2/1 Primary culture, Fura red and Fluo-3 (“Imaris”, Bitplane AG, Zürich)

2/2 Fluorescence and brightfield (Dr. Nitschke, Freiburg)

2/3 Drosophila embryo, early stage of development, double fluorescence, FITC (green) indicates Actin filaments, PI (red) shows RNA. (Dr. E. Schejter, Tel Aviv, Israel)

7/1 Live colon cells, SNARF-1 (Dr. Montrose, JH University, Baltimore)

7/2 Neuroglia cells in culture, 7/3 Membrane labelled Astrocytes (FITC), Oligodendrocytes connective tissue cells, (Rhodamine), nuclei (DAPI), Dr. M. 3D projection Bastmeyer, Uni Konstanz, (Dr. H. Schaden, Carl Zeiss Jena GmbH)

2/4 3T3 cell culture, TMRE, Calcium Green (Dr. Sparagna, UCT Farmington)

2/5 Cytokeratins and desmoplacine (Dr. Kartenberg, Heidelberg)

2/6 Cell walls and chloroplasts, double fluorescence

7/4 Mouse fibroblasts, cytoskeleton structures (Dr. Iwig, University of Halle)

7/5 Mouse fibroblasts, actin filaments (Dr. Iwig, University of Halle)

7/6 Fibroblast, microtubuli, intermediary filaments, nucleoli

2/7 Cell nuclei, cytokeratins and desmoplacine (Dr. Kartenberg, Heidelberg)

2/8 Drosophila embryo, cytoskeleton (Dr. Zhang, Duke University, Durham)

2/9 Tissue section, multiple fluorescence (M. Günthert, ETH Zürich)

7/7 Mitotic cells, multiple fluorescence (NCI Maryland)

7/8 Cultured cells, multiple fluorescence (Prof. Wunderli-Allenspach, ETH Zürich)

7/9 Cell culture, 3D shadow projection (calculated by “Imaris”, Bitplane AG, Zürich) showing tight junctions (red) and cytoskeleton structures (green). (Prof. Wunderli-Allenspach, ETH Zürich)

Printed on environment-friendly paper, bleached without the use of chlorine.

For further details, please contact:

Carl Zeiss Microscopy D-07740 Jena Phone: ++49-36 41/64 -1616 Telefax: ++49-36 41/64 -3144 E-mail: [email protected] Internet: www.zeiss.de/micro

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