LABORATORY SAFETY & MANAGEMENT DEPARTMENT OF MICRO & NANOSYSTEMS OSQULDAS VÄG 6, PLAN 5

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Contents DEPARTMENT OF MICRO & NANOSYSTEMS ......................................................... 1 OSQULDAS VÄG 6, PLAN 5 ................................................................................... 1 I. General Information................................................................................................ 4 1 Emergency ......................................................................................................... 4 2 Laboratory Authorities ........................................................................................ 5 3 Access to the Laboratory .................................................................................... 5 4 The Lab Manual .................................................................................................. 5 5 The Laboratory ................................................................................................... 5 II.

Safety & Protection ............................................................................................... 8 1 General Behaviour & Considerations ................................................................. 8 2 Personal Protection ............................................................................................ 9 3 Working alone in the lab ..................................................................................... 9

III.

Accidents ............................................................................................................. 9

1 General ............................................................................................................... 9 2 Fire ..................................................................................................................... 9 3 Eye damage ..................................................................................................... 10 4 Skin damage ..................................................................................................... 10 5 Inhalation of toxic gases & chemicals ............................................................... 10 6 Ingestion of chemicals ...................................................................................... 10 7 How to induce vomit ......................................................................................... 11 8 Injuries caused by electricity ............................................................................. 11 9 Shock ................................................................................................................ 11 IV. Working with Chemicals ..................................................................................... 12 1 MSDS ............................................................................................................... 12 2 Guidelines for chemicals .................................................................................. 12 3 Guidelines for combustible goods & organic solvents ...................................... 13 V. Working with Biological Samples ........................................................................ 14 VII. Waste Disposal Guide ...................................................................................... 14 1 General waste .................................................................................................. 14 2 Contaminated solid waste ................................................................................ 14 3 Liquid chemical Waste ...................................................................................... 14 4 Glass ................................................................................................................ 14 5 Sharps .............................................................................................................. 15 6 Batteries ........................................................................................................... 15 Appendix 1 ................................................................................................................. 16 1 Laboratory Authorities ...................................................................................... 16

3 2 Instrument Responsibles .................................................................................. 16 Contact angle measurement set-up ................................................................... 16 Laser cutter and laminator .................................................................................. 16 Speed mixer ....................................................................................................... 16 UV Light Source ................................................................................................. 16 Microscopes ....................................................................................................... 16 Scales ................................................................................................................. 17 Dessicators ......................................................................................................... 17 Spinner ............................................................................................................... 17 Ultrasonic bath .................................................................................................... 17 Fume hoods ........................................................................................................ 17 Laminar Flow Bench ........................................................................................... 17 Plasma Cleaner .................................................................................................. 17 Vacuum chamber and vacuum pumps ............................................................... 17 Ovens ................................................................................................................. 18 Fridges and Freezer ........................................................................................... 18 3D printer ............................................................................................................ 18 2-photon laser ..................................................................................................... 18

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I.

General Information

1 Emergency LAB ADDRESS: Osquldas väg 6, 5th floor. EMERGENCY NUMBERS:

112

Ambulance

112

Fire

112

Police

08 790 77 00

KTH Alarm (open 24 hrs)

112

Swedish Poison Information Centre (emergency)

08 33 12 31

Swedish Poison Information Centre (non-emergency)

08 32 01 00

Health Care Contact (Vårguiden)

08 672 31 00

Eye damage, St Eriks Ögonsjukhus (emergency)

CRISIS PLAN in case of emergency: 1. SAVE A first-aid kit is available in the printer room (see map for locations). Emergency eye-wash stations are located in the assembly lab and in the chemistry lab (see map). 2. ALARM Call 112. Tell Emergency Services: what has happened, where it has happened, and where you are calling from. Remain calm and describe clearly what has happened. Also inform them that there are gas bottles in the lab (and what type of gas). Inform KTH Alarm (08 790 77 00). 3. EXTINGUISH A fire extinguisher can be found outside the chemistry lab, in the electrical lab, and in the hallways close to each entrance/exit. 4. EVACUATE Evacuation: gather outside the KTH-Hallen on Brinellvägen 38.

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2 Laboratory Authorities The lab authorities are: •

Lab Safety Responsible

•

Lab Moderator(s)

•

Fire Safety Responsible

•

First-aid Operator(s)

•

Instrument Responsibles

You can find who currently covers these roles in the Appendix to this Manual. You must consult the lab authoritis regarding any laboratory and safety issue.

3 Access to the Laboratory Every Lab user must have a basic knowledge of laboratory procedures. To have access to the lab, every user has to pass the Initial training. The steps to be certified are: 1. 2. 3. 4.

Read, understand, and sign the safety routines in this Manual Pass a written test on the content of the Manual Attend a lab tutorial conducted by a lab responsible Pass a practical test consisting in documenting an experiment, assessing risk levels, conducting basic weighing and mixing steps, labelling properly, placing in storage, and disposing of waste

In the case of repeated poor lab behavior, the Initial training, in all its steps, will have to be repeated before the user is allowed back into the lab.

4 The Lab Manual A copy of this Manual is available in a binder in the lab, which also contains copies of the agreements signed by the lab users. The Manual is also available online in serverix. In the following, the general lab routines for users of the MST lab, Osquldas v. 6, floor 5, are described. It is not only important that you follow these routines, but also that you understand why things are done the way described. It is of highest importance that all users are aware of their responsibility in the lab, and really think through the risks in their activities in order to prevent accidents.

5 The Laboratory All lab doors must be kept unblocked at all times in case the lab needs to be evacuated. The floor map below shows the location of: •

Fire extinguishers

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Eye wash stations First-aid kit

II. Safety & Protection 1

General Behaviour & Considerations

It is responsibility of each lab user to plan and conduct his/her work in a safe way, without causing danger or disturbing the other people or the environment inside and outside the laboratory. When introducing a new lab procedure or new chemicals, the safety aspects must be considered. This must be done BEFORE starting the experiments or ordering the new chemicals. All chemicals and procedures must be approved by the Lab Safety Responsible. The following are strictly forbidden in all laboratory spaces: − Smoking − Eating/drinking − Obstructing walkways and common areas − Children & pets − Mouth pipetting − Touching doorknobs or leaving the lab with gloves on − Any lab activity outside the laboratory Each lab user is responsible for: − Reading and understanding this Manual − Knowing the safety protocols for emergencies − Following proper lab routines, according to this Manual − Handling all equipment is in a proper way − Keeping common workspaces free from unnecessary consumables − Tidying up after use − Labeling ALL samples and containers with name, date, and content − Always asking before borrowing tools from others − Returning all tools borrowed to their rightful place − Wearing proper lab gear (see below) − Reporting any spill or accident to the Lab Moderator − Handling all waste according to the Waste Disposal Guide (see VII)

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Personal Protection

Whenever in the lab, proper lab gear must be worn. The lab gear consists in: lab coat, gloves, covered shoes, and, when needed, goggles. Always wash hands thoroughly after leaving the laboratory.

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Working alone in the lab

It is not allowed to work alone in the laboratory, unless someone else is present in the department and aware of your activity in the lab.

III. Accidents There are two first-aid trained people currently employed at MST (see Appendix 1).

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General

The Material Safety Data Sheet (MSDS) of all the chemicals used in the lab are located, in paper form, in in the MSDS binder in the lab and can be found online in the KLARA database. All employees MUST report all accidents, spills, and near accidents that occur in the department to the Lab Moderator. Broken equipment and malfunctions must be reported to the respective Instrument Responsible, as well as to the Lab Moderator.

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Fire

All solvents are easy to ignite. Read the MSDS before using them. Keep solvents away from heat- or spark-generating objects. In case of a small fire, use a fire extinguisher. Act according to the Crisis Plan: 1. 2. 3. 4.

Save Alarm Extinguish Evacuate

If there is time, bring gas bottles to safety. In case of a large fire: 1. Alarm 2. Evacuate There is no fire alarm in the Q buildings. Spread the warning by word to all personnel on all floors! Evacuation: gather outside the KTH-Hallen on Brinellvägen 38.

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Eye damage

All chemicals should be regarded as potentially harmful. If you get anything in your eye, rinse abundantly with water. The eye must be open when rinsing. There is an eye-wash station located in the chemical laboratory (see map). Acids and Bases 1. Rinse eyes immediately with water for 15 min 2. Call the doctor UV Light Damage 1. Cover the eyes 2. Go to the emergency hospital

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Skin damage

All chemicals should be regarded as potentially harmful. If your skin has been exposed to any chemical or solvent, rinse abundantly with water. Weak Acids or weak Bases 1. Rinse skin with water and remove all contaminated clothing 2. Call the doctor Burns Minor burns must be rinsed with water until pain decreases. Do not puncture blisters. Major burns must be covered with sterile compress and the victim transported to the hospital. Risk for shock exists (see Shock).

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Inhalation of toxic gases & chemicals

No toxic-gas-generating activities are allowed. Consult the Lab Moderator before conducting experiments where toxic gas may be produced.

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Ingestion of chemicals

All chemicals should be regarded as potentially toxic. The Swedish Poison Information Centre (see I.1) should always be contacted if a chemical has been ingested.

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Acids, Bases and Ammonia Do NOT induce vomit! 1. Evaluate respiratory function and pulse 2. Ensure that the respiratory airway is not obstructed 3. Transport immediately to a hospital Salts of heavy metals (arsenic, barium, lead, mercury) 1. Induce vomit 2. Transport immediately to a hospital

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How to induce vomit

Vomit must never be induced in an unconscious person when acids or bases have been ingested! Vomit is induced by giving the victim a glass of milk or water, then putting two fingers in his/her throat as deep as possible and keeping them there until vomit is induced.

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Injuries caused by electricity

Immediately disconnect the electricity by turning off the main switch or by causing a short circuit. 1. Keep the victim warm and quiet 2. Give artificial respiration if needed 3. Transport immediately to a hospital

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Shock

Shock can arise from several causes, e.g. from burns, poisoning, or major bleeding. The condition is serious and implies that there has been a strong effect on blood circulation. Symptoms include paleness, cold sweat on the forehead and in the palms, ache, and indisposition. Never give the victim anything to drink! 1. Place the victim with his/her feet high and in a warm room free from drafts 2. Keep the respiratory airway free 3. Keep the victim warm with blankets 4. Call the ambulance immediately 5. Never leave the victim alone

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IV. Working with Chemicals 1

MSDS

All chemicals are to be regarded as being potentially harmful to health and should be used with proper precautions. Always read the Material Safety Data Sheet (MSDS) before working with chemicals. The Material Safety Data Sheet (MSDS) of all the chemicals used in the lab are located, in paper form, in in the MSDS binder in the lab and can be found online in the KLARA database. KLARA can be found by searching on the KTH website or asking Alexander Vastesson where to find it. The MSDS contain information about the classification of chemicals and their danger to health and/or environment. Make yourself aware of proper chemical handling before starting any experiments.

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Guidelines for chemicals

Be aware of the increased risk for accidents and mistakes when routine work gradually builds up a feeling of skillfulness and monotony. A person may become so familiar with the daily work that the possible hazards with handling chemicals might be forgotten.

13 Dangerous chemicals must be stored in fire resistant lockable cabinets with continuous ventilation. Flammable, oxidizing, toxic, acidic, or basic compounds must be clearly labeled as such. − Gloves must ALWAYS be used when handling chemicals − Do not store chemicals above head height or on the floor − When the handling of chemicals might result in contamination of the air by healthdamaging or explosive dusts, vapors, fumes, or gases, the work must be carried out in a fume hood − Chemicals must not be stored in the fume hoods − All bottles, flasks, tubes, beakers, etc. containing chemicals or preparations must be properly and clearly labeled with name, date, and content. − Never return used chemicals to their original container (as soon as the chemicals leave their containers they are considered to be contaminated), but dispose of the excess according to the Waste Disposal Guide (see page VII) − Avoid exposing chemicals and solvents to heat or direct sunlight − Pipetting solutions by mouth is absolutely forbidden − Keep your hands away from mouth and face when handling chemicals − Wash your hands after handling chemicals and right after leaving the lab to avoid contamination in offices and common areas − Never transport open beakers, bottles, tubes, etc. outside the lab − Separate acids from bases − Separate bases from acids and from easily ignitable materials

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Guidelines for combustible goods & organic solvents

− Combustible goods are labeled with a flame symbol − Exercise great care when handling combustible goods − Always handle combustible goods in a fume hood − Keep sources of ignition away (electrical equipment, flammable gas, etc.) − Combustible goods must be stored in a spark-protected refrigerator or freezer − Gas cylinders must be anchored − Never use spray bottles for ethanol (due to risk of aerosol formation), unless required by experimental protocols (disinfection of surfaces)

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V. Working with Biological Samples Inform the Safety Responsible and the Lab Moderator before working with biological samples. Use personal protection (lab coat, gloves, etc.) when handling biological samples. Always label all samples with name, date, and content. Use ethanol to disinfect and sterilize surfaces.

VII. Waste Disposal Guide 1

General waste

Clean gloves, paper tissues, plastic disposables (pipettes, petri dishes, spatulas, etc.), and packaging material can be thrown in the General waste bin.

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Contaminated solid waste

Contaminated paper tissues and plastic disposables (pipettes, petri dishes, spatulas, etc.) must be disposed in a single-use small plastic bag, which must be knotted close and thrown in the Solid organic waste bin. Contaminated gloves can be thrown directly in the Solid waste bin.

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Liquid chemical Waste

Never pour chemicals in any sink. Dispose each type of chemical in the respective waste container: •

Organic solvents (acetone, isopropanol, toluene, methanol, etc.)

•

Acids

•

Bases

Wash beakers/bottles with detergent and rinse with water at the wash station. Never mix acids and bases in the same container.

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Glass

Glass bottles, glass beakers, glass slides and fragments, and silicon pieces must be disposed in the Glass waste bin. Be sure to empty the glass bottles and rinse the glassware from any chemicals before disposing. Only put glass waste in the Glass waste bin.

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Sharps

Sharp and cutting objects such as needles, scalpels, and blades must be disposed in the Sharp container. Everything that has been in contact with biological samples (e.g. blood, tissue, etc.) must be disposed in the Sharp container as well.

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Batteries

Used batteries must be disposed in the batteries collection box placed in the Measurement lab (see map).

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Appendix 1 1

Laboratory Authorities

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Lab Safety Responsible: Tommy Haraldsson

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Lab Moderators: Laila Ladhani, Federico Ribet, Reza Shafagh, Xiamo Zhou, Alexander Vastesson, Gabriel Lenk

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Fire Safety Responsible: Mikael Bergqvist

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First-aid Operators: Erika Appel (also resuscitation-trained), Lab Moderators

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Instrument Responsibles: see below

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Instrument Responsibles

Contact angle measurement set-up Special training by the Responsible is required for this instrument. Responsible: Xiamo “Chianty” Zhou Safety concerns: Laser cutter and laminator Special training by the Responsible is required for this instrument. Responsible: Gabriel Lenk Safety concerns: Speed mixer Special training by the Responsible is required for this instrument. Responsible: Xiamo “Chianty” Zhou Safety concerns: UV Light Source Responsible: Xiamo “Chianty” Zhou Safety concerns: Microscopes Responsible: Alexander Vastesson, Jonas Hansson Safety concerns:

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Scales Responsible: Gabriel Lenk Safety concerns: Dessicators Responsible: Jonas Hansson Safety concerns: Spinner Responsible: Jonas Hansson Safety concerns: Ultrasonic bath Responsible: Reza Shafagh Safety concerns: Fume hoods Responsible: Alexander Vastesson Safety concerns: Chemical handling. Laminar Flow Bench Responsible: Gaspard Pardon Safety concerns: Plasma Cleaner Special training by the Responsible is required for this instrument. Responsible: Xuge Fan, Miku Lakso Safety concerns: Vacuum chamber and vacuum pumps Responsible: Xuge Fan, Miku Lakso Safety concerns:

18 Ovens Responsible: Jonas Hansson Safety concerns: Fridges and Freezer Responsible: Reza Shafagh Safety concerns: 3D printer Responsible: Gabriel Lenk Safety concerns: 2-photon laser Special training by the Responsible is required for this instrument. Responsible: Miku Laasko Safety concerns: