JOURNAL OF CLINICAL MICROBIOLOGY Volume 49

August 2011

No. 8

PHOTO QUIZ A Rare Presentation of a Common Disease

Neil Anderson, Nagarjun Rao, and Nathan A. Ledeboer

2785

Answer to Photo Quiz

Neil Anderson, Nagarjun Rao, and Nathan A. Ledeboer

3112

BACTERIOLOGY Francisella tularensis Molecular Typing Using Differential Insertion Sequence Amplification

Marilynn A. Larson, Paul D. Fey, Amanda M. Bartling, Peter C. Iwen, Michael P. Dempsey, Stephen C. Francesconi, and Steven H. Hinrichs

2786–2797

Practical Approach for Reliable Detection of AmpC BetaLactamase-Producing Enterobacteriaceae

Silke Polsfuss, Guido V. Bloemberg, Jacqueline Giger, Vera Meyer, Erik C. Bo ¨ttger, and Michael Hombach

2798–2803

Comparative Evaluation of Combined-Disk Tests Using Different Boronic Acid Compounds for Detection of Klebsiella pneumoniae Carbapenemase-Producing Enterobacteriaceae Clinical Isolates

Athanassios Tsakris, Katerina Themeli-Digalaki, Aggeliki Poulou, Georgia Vrioni, Evangelia Voulgari, Vasiliki Koumaki, Antonella Agodi, Spyros Pournaras, and Danai Sofianou

2804–2809

Clonal Evolution Leading to Maintenance of Antibiotic Resistance Rates among Colonizing Pneumococci in the PCV7 Era in Portugal

Alexandra S. Simo ˜es, Liliana Pereira, So ´nia Nunes, Anto ´nio Brito-Avo ˆ, Hermínia de Lencastre, and Raquel Sa´-Lea˜o

2810–2817

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

Li Xiao, Vanya Paralanov, John I. Glass, Lynn B. Duffy, Janet A. Robertson, Gail H. Cassell, Yuying Chen, and Ken B. Waites

2818–2826

Comparison of Direct Colony Method versus Extraction Method for Identification of Gram-Positive Cocci by Use of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Adnan A. Alatoom, Scott A. Cunningham, Sherry M. Ihde, Jayawant Mandrekar, and Robin Patel

2868–2873

Collaborative Evaluation of an Erythromycin-Clindamycin Combination Well for Detection of Inducible Clindamycin Resistance in Beta-Hemolytic Streptococci by Use of the CLSI Broth Microdilution Method

James H. Jorgensen, M. Leticia McElmeel, Letitia C. Fulcher, Lesley McGee, Sandra S. Richter, K. P. Heilmann, Mary Jane Ferraro, Jean Spargo, and Anita Glennen

2884–2886

Impact of Clinical Symptoms on Interpretation of Diagnostic Assays for Clostridium difficile Infections

Erik R. Dubberke, Zhuolin Han, Linda Bobo, Tiffany Hink, Brenda Lawrence, Susan Copper, Joan Hoppe-Bauer, Carey-Ann D. Burnham, and William Michael Dunne, Jr.

2887–2893

Evaluation of Commercial Universal rRNA Gene PCR plus Sequencing Tests for Identification of Bacteria and Fungi Associated with Infectious Endocarditis

Christian Ku ¨hn, Claudia Disque´, Helge Mu ¨hl, Peter Orszag, Meike Stiesch, and Axel Haverich

2919–2923

Detection of AmpC Beta-Lactamase in Escherichia coli: Comparison of Three Phenotypic Confirmation Assays and Genetic Analysis

S. Peter-Getzlaff, S. Polsfuss, M. Poledica, M. Hombach, J. Giger, E. C. Bo ¨ttger, R. Zbinden, and G. V. Bloemberg

2924–2932

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Harnessing the Glucosyltransferase Activities of Clostridium difficile for Functional Studies of Toxins A and B

Charles Darkoh, Heidi B. Kaplan, and Herbert L. DuPont

2933–2941

Rapid Genoserotyping Tool for Classification of Salmonella Serovars

Kristyn Franklin, Erika J. Lingohr, Catherine Yoshida, Muna Anjum, Levente Bodrossy, Clifford G. Clark, Andrew M. Kropinski, and Mohamed A. Karmali

2954–2965

Amyloid Histology Stain for Rapid Bacterial Endospore Imaging

Bing Xia, Srigokul Upadhyayula, Vicente Nun ˜ez, Pavel Landsman, Samuel Lam, Harbani Malik, Sharad Gupta, Mohammad Sarshar, Jingqiu Hu, Bahman Anvari, Guilford Jones, and Valentine I. Vullev

2966–2975

Genetic Characterization of Multidrug-Resistant, ExtendedSpectrum-␤-Lactamase-Producing Vibrio cholerae O1 Outbreak Strains, Mpumalanga, South Africa, 2008

Husna Ismail, Anthony M. Smith, Arvinda Sooka, and Karen H. Keddy for the Group for Enteric, Respiratory, and Meningeal Disease Surveillance in South Africa (GERMS-SA)

2976–2979

Utility of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification

Stephen A. Neville, Annabelle LeCordier, Helen Ziochos, Mathew J. Chater, Iain B. Gosbell, Michael W. Maley, and Sebastiaan J. van Hal

2980–2984

New Diagnostic Microarray (Check-KPC ESBL) for Detection and Identification of Extended-Spectrum BetaLactamases in Highly Resistant Enterobacteriaceae

Ina Willemsen, Ilse Overdevest, Nashwan al Naiemi, Martine Rijnsburger, Paul Savelkoul, Christina Vandenbroucke-Grauls, and Jan Kluytmans on behalf of the TRIANGLe Study Group

2985–2987

Performance of the LightCycler SeptiFast Test Mgrade in Detecting Microbial Pathogens in Purulent Fluids

Silvia Sancho-Tello, Dayana Bravo, Rafael Borra´s, Elisa Costa, Beatriz Mun ˜oz-Cobo, and David Navarro

2988–2991

Frequency of Panton-Valentine Leukocidin-Producing Methicillin-Sensitive Staphylococcus Strains in Patients with Complicated Skin and Soft Tissue Infection in Bronx, New York

Anna Kaltsas, Alice Guh, Jose´ R. Mediavilla, Avanish K. Varshney, Natalie Robiou, Philip Gialanellia, Michael Henry, Michael H. Levi, and Bettina C. Fries

2992–2995

Prevalence and Genetic Relatedness of MethicillinSusceptible Staphylococcus aureus Isolates Detected by the Xpert MRSA Nasal Assay

Sophie S. Arbefeville, Kunyan Zhang, Jennifer S. Kroeger, Wanita J. Howard, Daniel J. Diekema, and Sandra S. Richter

2996–2999

Sequence Analysis of the p1 Adhesin Gene of Mycoplasma pneumoniae in Clinical Isolates Collected in Beijing in 2008 to 2009

Fei Zhao, Bin Cao, Jing Li, Sufan Song, Xiaoxia Tao, Yudong Yin, Lihua He, and Jianzhong Zhang

3000–3003

Evaluation of Matrix-Assisted Laser Desorption Ionization– Time of Flight Mass Spectrometry for Rapid Identification of Beta-Hemolytic Streptococci

Abdessalam Cherkaoui, Ste´phane Emonet, Jose´ Fernandez, Didier Schorderet, and Jacques Schrenzel

3004–3005

Aeromonas aquariorum Is Widely Distributed in Clinical and Environmental Specimens and Can Be Misidentified as Aeromonas hydrophila

Max Aravena-Roma´n, Gerald B. Harnett, Thomas V. Riley, Timothy J. J. Inglis, and Barbara J. Chang

3006–3008

Determination of Disk Diffusion and MIC Quality Control Guidelines for JNJ-Q2, a Novel Quinolone

James E. Ross, Lisa C. Liverman, and Ronald N. Jones

3009–3011

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Emerging Severe and Fatal Infections Due to Klebsiella pneumoniae in Two University Hospitals in France

Dominique Decre´, Charlotte Verdet, Aure´lie Emirian, Thibault Le Gourrierec, Jean-Claude Petit, Georges Offenstadt, Eric Maury, Sylvain Brisse, and Guillaume Arlet

3012–3014

Clinical and Microbiological Characteristics of CommunityAcquired Staphylococcus lugdunensis Infections in Southern Taiwan

An-Bang Wu, Ming-Cheng Wang, Chin-Chung Tseng, Wei-Hung Lin, Ching-Hao Teng, Ay-Huey Huang, Kuei-Hsiang Hung, Chuan ChiangNi, and Jiunn-Jong Wu

3015–3018

Adherence to CLSI Recommendations for Testing of Staphylococcus aureus Isolates in Louisiana Hospitals: Report of a Clinical Failure and Results of a Questionnaire Study

Kamakshya P. Patra, John A. Vanchiere, and Joseph A. Bocchini, Jr.

3019–3020

Use of Filter Paper as a Transport Medium for Laboratory Diagnosis of Cholera under Field Conditions

Anne-Laure Page, Kathryn P. Alberti, Alain Gue´nole´, Vital Mondongue, Sylvaine Lonlas Mayele, Philippe J. Guerin, and Marie-Laure Quilici

3021–3023

Multiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcus aureus and Associated Toxin Genes

G. E. Fosheim, A. C. Nicholson, V. S. Albrecht, and B. M. Limbago

3071–3073

Efficient Direct Extended-Spectrum ␤-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers

Justin Ellem, Sally R. Partridge, and Jonathan R. Iredell

3074–3077

High-Resolution Genotyping of Chlamydia trachomatis by Use of a Novel Multilocus Typing DNA Microarray

Linus Christerson, Anke Ruettger, Kirsten Gravningen, Ralf Ehricht, Konrad Sachse, and Bjo ¨rn Herrmann

2838–2843

Evaluation of High-Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples

Reinier J. M. Bom, Linus Christerson, Maarten F. Schim van der Loeff, Roel A. Coutinho, Bjo ¨rn Herrmann, and Sylvia M. Bruisten

2844–2853

Suitability of Xpert MTB/RIF and Genotype MTBDRplus for Patient Selection for a Tuberculosis Clinical Trial

Sven O. Friedrich, Amour Venter, Xavier A. Kayigire, Rodney Dawson, Peter R. Donald, and Andreas H. Diacon

2827–2831

Evaluation of Two Molecular Assays for Rapid Detection of Mycobacterium tuberculosis Resistance to Fluoroquinolones in High-Tuberculosis and -Multidrug-Resistance Settings

I. Kontsevaya, S. Mironova, V. Nikolayevskyy, Y. Balabanova, S. Mitchell, and F. Drobniewski

2832–2837

Molecular Detection and Identification of Mycobacterium tuberculosis Complex and Four Clinically Important Nontuberculous Mycobacterial Species in Smear-Negative Clinical Samples by the GenoType Mycobacteria Direct Test

Can Bicmen, Ayriz T. Gunduz, Meral Coskun, Gunes Senol, A. Kadri Cirak, and Ayse Ozsoz

2874–2878

Incidence of Moxifloxacin Resistance in Clinical Mycobacterium tuberculosis Isolates in Houston, Texas

Hana M. El Sahly, Larry D. Teeter, Kenneth C. Jost, Jr., Denise Dunbar, Justin Lew, and Edward A. Graviss

2942–2945

Controlled Comparison of BacT/Alert MB System, Manual Myco/F Lytic Procedure, and Isolator 10 System for Diagnosis of Mycobacterium tuberculosis Bacteremia

John A. Crump, Anne B. Morrissey, Habib O. Ramadhani, Boniface N. Njau, Venance P. Maro, and L. Barth Reller

3054–3057

CHLAMYDIOLOGY AND RICKETTSIOLOGY

MYCOBACTERIOLOGY AND AEROBIC ACTINOMYCETES

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Evaluation of Oral Antiseptic Rinsing before Sputum Collection To Reduce Contamination of Mycobacterial Cultures

Renata L. Peres, Moise´s Palaci, Rafaela B. Loureiro, Reynaldo Dietze, John L. Johnson, Jonathan E. Golub, A. Ruffino-Netto, and Ethel L. Maciel

3058–3060

Preanalytical Delay Reduces Sensitivity of QuantiFERONTB Gold In-Tube Assay for Detection of Latent Tuberculosis Infection

David Doberne, Rajiv L. Gaur, and Niaz Banaei

3061–3064

Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis

Manuel Causse, Pilar Ruiz, Juan Bautista Gutie´rrez-Aroca, and Manuel Casal

3065–3067

Cepheid GeneXpert MTB/RIF Assay for Mycobacterium tuberculosis Detection and Rifampin Resistance Identification in Patients with Substantial Clinical Indications of Tuberculosis and Smear-Negative Microscopy Results

Panayotis Ioannidis, Dimitrios Papaventsis, Simona Karabela, Stavroula Nikolaou, Marina Panagi, Ekaterini Raftopoulou, Eythymia Konstantinidou, Ioanna Marinou, and Sofia Kanavaki

3068–3070

Quantitation of Candida CFU in Initial Positive Blood Cultures

Christopher D. Pfeiffer, Gregory P. Samsa, Wiley A. Schell, L. Barth Reller, John R. Perfect, and Barbara D. Alexander

2879–2883

Colletotrichum truncatum: an Unusual Pathogen Causing Mycotic Keratitis and Endophthalmitis

M. R. Shivaprakash, Suma B. Appannanavar, Manpreet Dhaliwal, Amit Gupta, Sunita Gupta, Amod Gupta, and Arunaloke Chakrabarti

2894–2898

Antifungal Susceptibilities of Sporothrix albicans, S. brasiliensis, and S. luriei of the S. schenckii Complex Identified in Brazil

Daniele C. Oliveira, Paulo G. Markus Lopes, Tatiana B. Spader, Camila D. Mahl, Giordano R. Tronco-Alves, Valeria M. Lara, Janio M. Santurio, and Sydney Hartz Alves

3047–3049

Rapid Identification of Cryptococcus neoformans and Cryptococcus gattii by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Lisa R. McTaggart, Eric Lei, Susan E. Richardson, Linda Hoang, Annette Fothergill, and Sean X. Zhang

3050–3053

Edwin Kamau, LaDonna S. Tolbert, Luke Kortepeter, Michael Pratt, Nancy Nyakoe, Linda Muringo, Bernard Ogutu, John N. Waitumbi, and Christian F. Ockenhouse

2946–2953

Angela M. Caliendo, Alexander Valsamakis, James W. Bremer, Andrea Ferreira-Gonzalez, Suzanne Granger, Linda Sabatini, Gregory J. Tsongalis, Yun F. (Wayne) Wang, Belinda Yen-Lieberman, Steve Young, and Nell S. Lurain

2854–2858

MYCOLOGY

PARASITOLOGY Development of a Highly Sensitive Genus-Specific Quantitative Reverse Transcriptase Real-Time PCR Assay for Detection and Quantitation of Plasmodium by Amplifying RNA and DNA of the 18S rRNA Genes

VIROLOGY Multilaboratory Evaluation of Real-Time PCR Tests for Hepatitis B Virus DNA Quantification

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Identification of HIV Superinfection in Seroconcordant Couples in Rakai, Uganda, by Use of Next-Generation Deep Sequencing

Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients

Andrew D. Redd, Aleisha Collinson-Streng, Craig Martens, Stacy Ricklefs, Caroline E. Mullis, Jordyn Manucci, Aaron A. R. Tobian, Ethan J. Selig, Oliver Laeyendecker, Nelson Sewankambo, Ronald H. Gray, David Serwadda, Maria J. Wawer, Stephen F. Porcella, and Thomas C. Quinn on behalf of the Rakai Health Sciences Program ´ ngeles Clari, Dayana Bravo, María A Elisa Costa, Beatriz Mun ˜oz-Cobo, Carlos Solano, María Jose´ Remigia, and David Navarro

2859–2867

2899–2904

Evaluation of a New DNA Test for Detection of Carcinogenic Human Papillomavirus

Philip E. Castle, Erin C. Gutierrez, Sharon V. Leitch, Courtney E. Maus, Ray A. McMillian, William A. Nussbaumer, Laurence M. Vaughan, Cosette M. Wheeler, Patti E. Gravitt, and Mark Schiffman

3029–3032

Diagnostic Consequences of Cytomegalovirus Glycoprotein B Polymorphisms

Zdenek Novak, Nazma Chowdhury, Shannon A. Ross, Sunil K. Pati, Karen Fowler, and Suresh B. Boppana

3033–3035

Comparison of Serial Hepatitis C Virus Detection in Samples Submitted through Serology for Reflex Confirmation versus Samples Directly Submitted for Quantitation

Howard B. Gale, D. Robert Dufour, Nazia N. Qazi, and Virginia L. Kan

3036–3039

Hepatitis C Virus Genotypes in Clinical Specimens Tested at a National Reference Testing Laboratory in the United States

Jeffrey J. Germer, Jayawant N. Mandrekar, Jordan L. Bendel, P. Shawn Mitchell, and Joseph D. C. Yao

3040–3043

Precision across the Analytical Measuring Range of a Quantitative Real-Time PCR Assay for Cytomegalovirus Detection among Three Clinical Laboratories

Thomas E. Grys, Doreen L. Duquette, Bruce White, Cole Irish, D. Jane Hata, and Bobbi S. Pritt

3044–3046

Jan P. M. Langeveld, Jo H. F. Erkens, Ines Rammel, Jorg G. Jacobs, Aart Davidse, Fred G. van Zijderveld, Alex Bossers, and Hermann Schildorfer

3026–3028

María García-Castillo, Rosa del Campo, María Isabel Morosini, Elena Riera, Gabriel Cabot, Rob Willems, Rosa van Mansfeld, Antonio Oliver, and Rafael Canto ´n

2905–2910

CLINICAL VETERINARY MICROBIOLOGY Four Independent Molecular Prion Protein Parameters for Discriminating New Cases of C, L, and H Bovine Spongiform Encephalopathy in Cattle

EPIDEMIOLOGY Wide Dispersion of ST175 Clone despite High Genetic Diversity of Carbapenem-Nonsusceptible Pseudomonas aeruginosa Clinical Strains in 16 Spanish Hospitals

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Group B Streptococci Causing Neonatal Infections in Barcelona Are a Stable Clonal Population: 18-Year Surveillance

Use of Modified PCR Ribotyping for Direct Detection of Clostridium difficile Ribotypes in Stool Samples

E. R. Martins, A. Andreu, P. Correia, T. Juncosa, J. Bosch, M. Ramirez, and J. Melo-Cristino on behalf of the Microbiologist Group for the Study of Vertical Transmission Infections from the Catalan Society for Clinical Microbiology and Infectious Diseases ˇtrumbelj, and Sandra Janezˇicˇ, Iztok S Maja Rupnik

2911–2918

3024–3025

CASE REPORTS Prototheca wickerhamii Mimicking Yeast: a Cautionary Tale

B. McMullan, K. Muthiah, D. Stark, L. Lee, and D. Marriott

3078–3081

Identification by 16S rRNA Gene Sequencing of Negativicoccus succinicivorans Recovered from the Blood of a Patient with Hemochromatosis and Pancreatitis

D. L. Church, K. E. Simmon, Jan Sporina, T. Lloyd, and D. B. Gregson

3082–3084

Early Detection of Colonization by VIM-1-Producing Klebsiella pneumoniae and NDM-1-Producing Escherichia coli in Two Children Returning to France

Andre´ Birgy, Catherine Doit, Patricia Mariani-Kurkdjian, Nathalie Genel, Albert Faye, Guillaume Arlet, and Edouard Bingen

3085–3087

Unusual Case of Resistance to Amphotericin B in Visceral Leishmaniasis in a Region in India Where Leishmaniasis Is Not Endemic

Pankaj Srivastava, Vijay Kumar Prajapati, Madhukar Rai, and Shyam Sundar

3088–3091

Bacteremia with Bacteroides pyogenes after a Cat Bite

Ida Ringsborg Madsen and Ulrik Stenz Justesen

3092–3093

Travel-Related Streptococcal Toxic Shock Syndrome Caused by emm Type 78 Streptococcus pyogenes

Dennis Tappe, Marco H. Schulze, Mark van der Linden, Uwe Ziegler, Andreas Mu ¨ller, and August Stich

3094–3095

Prosthetic Joint Infection Caused by Mycobacterium alvei in an Elderly Patient

Chen-Hsiang Lee, Huey-Ling You, Jun-Wen Wang, Ya-Fen Tang, and Jien-Wei Liu

3096–3098

First Report of Community-Associated Methicillin-Resistant Staphylococcus aureus (USA300) in Mexico

María E. Velazquez-Meza, Jacobo Ayala-Gayta´n, María N. CarnallaBarajas, Araceli Soto-Noguero ´n, Claudia E. Guajardo-Lara, and Gabriela Echaniz-Aviles

3099–3100

A Synonymous Change in the Influenza A Virus Neuraminidase Gene Interferes with PCR-Based Subtyping and Oseltamivir Resistance Mutation Detection

Christina Trevino, Selamawit Bihon, and Benjamin A. Pinsky

3101–3102

Reduced Sensitivity of Oxacillin-Screening Agar for Detection of MRSA ST398 from Colonized Pigs

Efstathios S. Giotis, Anette Loeffler, Jodi A. Lindsay, and David H. Lloyd

3103–3104

Unusual Genotype of a Uropathogenic Escherichia coli Strain Assigned to the B2 Phylogenetic Group

Nuno Mendonc¸a, Vera Calhau, Tess Lin, Luísa Boaventura, Grac¸a Ribeiro, and Gabriela Jorge Da Silva

3105–3106

First Human Isolate of Mycobacterium poriferae in the Sputum of a Patient with Chronic Bronchitis

Frederic Ballester, Isabel Pujol, Fernando Alcaide, Isabel Pizarro, Josep M. Simo ´, Jorge Joven, and Jordi Camps

3107–3108

LETTERS TO THE EDITOR

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Why Should the Journal of Clinical Microbiology Apologize for Publishing a Paper Plagiarized from Its Archives?

Letter: M. Jacques Nsuami, Nsanga Besa, Yanyi K. Djamba, Emmanuel Sikabwe, and Wato Nsa. Reply: Gary V. Doern

3109–3110

ERRATUM Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Cervical Carcinomas from Patients in Northeast China

Qinglong Shang, Yan Wang, Yong Fang, Lanlan Wei, Sijia Chen, Yuhui Sun, Baoxin Li, Fengmin Zhang, and Hongxi Gu

3111