International Scientific Meeting 25 – 30 November 2014 Yogyakarta
MEDICINE TODAY CONVENTIONAL DISEASE SAKIT
NON CONVENTIONAL
Pathogenese
DIS-EASE SEHAT Physiogenese
♣ Naturopathic Medicine ♣ Anti Aging Medicine ♣ Wellness Medicine ♣ Regenerative Medicine ♣ Functional Medicine ♣ Restorative Medicine
DISEASE
DIS-EASE
PHYSIOGENESIS TARGET
HEALTH & MEDICAL CARE GRADING • TREATMENT • RECOVERY • REJUVENATION
• REVITALIZATION • RECUPERATION
FOOD SOURCES
NUTRACEUTICAL NUTRIGENOMIC STEM ENHANCER
NON FOOD SOURCES
PHYTOCEUTICAL PHYTOGENOMIC PHYTOSTEM-CEUTICAL
WELLNESS ORIENTED HOLISTIC PROGRAM THROUGH WELLBEING
NATUROCEUTICAL vs FUNCTIONAL FOOD Bio-active substance in food or part of a food provides medical or health benefits, including the prevention and treatment. Nutrient rich or medicinally active ingredients and range from isolated or purified nutrients to modern engineered designer foods
Ordinary food has components or ingredients added to give it a specific physiological benefit and must meet (1) present in their naturally-occurring form, (2) consumed in the diet as often as daily; and (3) should regulate a biological process of prevent or controlling disease
NATUROCEUTICAL
FUNCTIONAL FOOD
MEDICINAL FOOD
WELLNESS FOOD
PROVIDE A HEALTH & MEDICAL BENEFIT BEYOND BASIC NUTRITION
WE ARE WHAT WE EAT Food for life
Food for health
Food as medicine
Nutrient Requirement RDA or DRI AI or UL Digestive system
HEALTH EMPOWERMENT
6 7 1 5 4
2
3
CDC study • Poor diet overtake tobacco mortality rate • 16,6 death were related to poor diet • The leading preventable cause of death
National Cancer Institute study • 40% do not eat fruit • 20% do not have vegetable
US Dept. of Agriculture: food consumption survey • • • • •
3% met the Four Food Groups pattern 12% obtained of the RDA 30% less RDA for vitamin intake 70% eat less of micronutrients 90% less RDA for mineral intake
Havard University study on dietary trends 1860 - 1975 1860 Protein Fat Carbohydrate Complex Simple Total
12 25
1920 12 32
53 10 63
43 13 56
%
1925 12 35
1975 12-15 40-45
37 16 53
22 24 46
The Need for Supernutrition, , 1991
WHY FOOD IS NOT ENOUGH • Need more to return to optimum health • Degenerative process needs to corrected by flooding the body with nutrients • All of us have inherited genetic weakness • Free radical & life-styles needs extra nutrient
NATUROCEUTICAL DEGENERATIVE DISEASE
Disappearing Nutrients Bioavailability Soils Harvesting Transportation Storage Processing Washing Cooking
Micronutrient and Degenerative process
Disease related nutrition and genetic networking
Physiol. Genomics January 1, 2004 vol. 16 no. 2 161-165
ISOGENIC APPROACHES
J Nutr. 2005 Jul;135(7):1613-6 - Science Direct. Volume 21, Issue 6, June 2010, Pages 457–467
Naturoceutical
Molecular nutrition paradigm
Nat Rev Drug Discov 5, 993-996. (2006)
The Role of Naturoceutical for Biomolecular Cellular Activities
Courtesy from Barrat Aggrawal International Journal of Molecular Sciences 2012, 13(3), 3959-3978
NATUROCEUTICAL
Naturoceutical and cellular processes linked to degenerative
Naturoceutical
WCRF International
The basis study of Naturoceutical for cellular lifespan process
Naturoceutical
WCRF International
Naturoceutical substances Nutrients Phytochemicals Source of energy
Growth and Tissues maintenance
Regulation Body Process
Life sustaining functions Metabolisms Growth & Development
Body Composition
Organ & System fx
European Nutrigenomic Organization
PRIMARY
SECONDARY
Essential material
Chemical agent
Nutritive value
Pharmacological activity
BIO-NUTRITION
NATUROCEUTICAL
Life Extension Foundation
• Impossible to know how much of discovered nutrients in foods Food tables have not yet been prepared • In order to get adequate amount food must be concentrated Eat lots of those, the calories would be excessive
WELLNESS ORIENTED HOLISTIC PROGRAM THROUGH WELLBEING
• Several rich sources are critical nutrients not in conventional People tend to tire of such food rapidly • Some people already chosen not to change the diet Might consider adding supplements • Too busy to prepare new food Ultimate convenience, compatible and premeasured • Paramount importance for protection from major diseases Optimal nourishment will supercharge
Signal transduction
Y
Receptors
Y Y
Electron transport DNA - Replication - Transcription - DNA repair
Transporter
Ion channels
Protein biosynthesis
Mitochondrion
n u c l e u s
Enzymes
RNA
lysosome
Cell membrane Cytoskeleton (actin, microtubules)
Posttranslational protein modification Van Wyk (2004)
Nutrition
http://www.ascendedhealth.com/concept.htm
Naturoceutical Delivery Actions
Advanced Drug Delivery Reviews. Available online 20 July 2012
Naturoceutical
LONGEVITY GENE & NATUROCEUTICAL PTMs
Interacting proteins
Parathymosine
Activator NAD
SIRT1 expression
Nicotinamide adenine dinucleotide
Sirtuin homolog 1
PGC1α
Peroxisome coactivator1 ● Gluconeogenesis ● Fatty acid oxidation
FOXO1
Forkhead box 01 ● Gluconeogenesis ● Insulin secretion ● Adiponectin production
SIRT1
+
NfkB
-
+
+
LXRs
+
STAT3
-
Nuclear factor kappa B
● Insulin secretion ● Insulin sensitivity
CRTC2 Creb Regulated Transcription Coactivator2
● GluconeogenesisB
Signal Tranducer Activator Of Transcription3
Liver X receptor ● Gluconeogenesis ● Lipid metabolism AceCS1 Acetyl CoA synthetase ● Acetyl CoA scavenger Pharmacological Research. Volume 62, Issue 1, July 2010, Pages 35–41
Biochemical and Biophysical Research Communications Volume 378, Issue 1, 2 January 2009, Pages 6–9
Naturoceutical
Aggarwal BB, et al Adv Exp Med Biol. 2007;595:1-75
Plant-base Naturoceutical Phytoactive Phyto-allel
Phytonutrient Phytochemical Phytoenzyme Phytohormone
DNA targets Intercalate DNA Alkylate DNA Inhibit DNA Inhibit RNA enzyme Protein biosynthesis Modulate metabolic enzyme Disturb membrane activity
HEALTHY CELL Miller G, 1997 – J of Am Col Nutr;16: 293-295
Supply of methyl groups inhibit DNA methylation processes Modify the use of methyl groups DNA methyltransferase reaction Response to bioactive components influence DNA demethylation Ability to interact at molecular level DNA methylation patterns Methylation Switching genes on or off which alters genetic messages and modify genetic expression
Epigenomic
CH3-DNA
Nutrigenetic
Nutrigenomic
DNA
mRNA
Bioactive Food Components
Post translational
Proteins
Enzyme, Structural Transport, Signaling caused by Phosphorylation and Glycocylation
Phenotype
Functional modules
Metabolic pathways
Genes
mRNA
Proteins
Metabolites
5 tenets of GENE NOURISHMENTS 1. Under certain circumstances diet can be a serious risk factor for a number of diseases. 2. Common dietary chemicals can act on the human genome (directly or indirectly), to alter gene expression or structure. 3. The degree to which diet influences the balance between healthy and disease states may depend on an individual’s genetic makeup. 4. Some diet-regulated genes are likely to play a role in the onset, incidence, progression, and/or severity of chronic diseases. 5. Dietary intervention based on knowledge of nutritional requirement, nutritional status, and genotype (i.e., "personalized nutrition") can be used to prevent, mitigate or cure chronic disease.
“Medicine and food are isogenic” ABSOLUTE INTAKE
Bioactive ability Cell to cell behave Genomic variation PHYSIOLOGICAL CHANGE Belury M.A. 2002. J Nutr. 132 : 2995-2998
Interaction nutrients with genetic and epigenetic as to lead to a phenotype change
NUTRIENT SPECIFIC BIOAVAILABILITY NEGATIVE EFFICACY BIOLOGICAL MODIFICATION SUPPORT DETOXYGENIC ENZYME BLOCK CARCINOGENIC BUILD UP HORMONAL HOMEOSTASIS CHANGE DELAY CELL DIVISION REDUCE APOPTOSIS
GENETIC MANIPULATION Int’l Life Science Symposium, Paris 2001
≈ FUNCTIONAL FOOD Nutrient that can positively change one or more target function besides nutritional effects as to improve health and well-being
Nutrient that could modulate body functions • • • • •
A NATURAL FOOD SOURCE SUPPLEMENTED BY COMPONENT NO LONGER HOLDING COMPONENT COMPONENT HAD BEEN CHANGED AVAILABILITY HAD BEEN CHANGED F. Marota et al, 2005 Midd E J of Age and Aging; 3: 1-6
Nutrients can affect GENE function The nutrients you ingest from foods can have direct effects on gene expression and activity in three ways:
Directly turn on or off a gene’s activity Change enzyme activity in the metabolic pathways of gene regulation
Interact with end product that gene makes Nutrients can control gene expression, function and metabolism to benefit our health and longevity
Phenotype
Functional modules
Metabolic pathways
Genes
mRNA
Proteins
Metabolites
NUTRAGENOMIC APPROACHES
Plant secondary metabolite Phytoactive
Phytonutrient Phytochemical Phytoenzyme Phytohormone
Phyto-allel Human cells & genes • • • • •
Gene Gene Gene Gene Gene
transcription modulation regulation translation expression
Recuperation Rejuvenation Revitalization Miller G, 1997 – J of Am Col Nutr;16: 293-295
GENE PROTECTOR
GENE REPAIR
Colour
Yellow, orange, bright red
Green, green/white
Bioactive
Carotenes, Lycopene, Lutein, Zeaxanthin
Cysteine, Isothiocyanate, Indol, Sulphoraphane
Serving
2 – 3 per day
2 – 4 per day
GENE EXPRESSION
GENE BUILDER
Colour
Purple, Blue, Black
Pale yellow, Reddish brown
Bioactive
Flavonoids, Anthocyanidine, Resevratol
Essential Fatty Acids, Amino Acids
Serving
1 – 2 per day
1 – 2 per day
