200 PERRY PARKWAY, SUITE 1 | GAITHERSBURG, MD | 20877 301.545.0238 PHONE | 888.545-0238 TOLL-FREE PHONE | 301.424.1989 FAX WWW.GLOBALSTEM.COM | [email protected] | [email protected]

INNOVATIVE PRODUCTS FOR NEUROSCIENCE RESEARCH

@ 2014 GLOBALSTEM. ALL RIGHTS RESERVED PRODUCTS FOR RESEARCH USE ONLY ● NOT FOR THERAPEUTIC OR DIAGNOSTIC USES ● NOVEMBER 2014 V3.1.0

NEURAL CELLS & MEDIA | STEM CELLS & CULTURE REAGENTS | TRANSFECTION | CUSTOM SERVICES

NEURAL CELL CULTURE

ABOUT US COMPANY OVERVIEW | WHO WE ARE

NEURAL CELL CULTURE | NEURAL MEDIA & SUPPLEMENTS Our neural basal medium and supplement are optimized for maximum growth and survival of primary and iPSC-derived neurons in culture. GS21™ Neural Supplement, a serum-free supplement formulation based

Since 2006, GlobalStem, a Molecular Transfer, Inc. company, has been discovering, developing, and commercializing

on the previously published formulation used with NeuralQ™ Basal Medium show superior performance in

innovative technologies and products for cell biology research. Our extensive customer base includes researchers from

supporting overall cell health and viability of neurons during short-term and long-term culture.

major academic, government and industrial institutions.

CORE COMPETENCIES | WHAT WE DO ACHIEVE MAXIMUM NEURON VIABILITY AND CELL GROWTH NEUROBIOLOGY     GS21™ Supplement              Top Compe tor 

Maximum cell viability during short-term and long-term culture Primary Rat Cortical Neurons (GSC-8220) cultured in GS21™

Compe tor 

 

GS21™ 

supplemented culture media showed significantly higher neuron viability after 2 weeks, p=0.032 and 4 weeks, p=0.005 in culture, as compared to cells cultured in top competitor supplement. Neurons

Our neurobiology portfolio includes neural cells, media and supplements to support neurotoxicity, drug screening or gene expression studies. Our proprietary cryopreservation process provides for consistent post-thaw viability of our neural cells. Researchers can confidently standardize their neural cell culture protocols with our high quality basal media and supplements. For your large-scale or technically challenging projects we offer custom services and products for neural cell isolation and differentiation. Contact us for more details.

plated in 96-well plates were labeled with MAP2 (green), and Dapi (blue) to denote nuclei. Data from n=4 wells is shown as mean ±

We have developed a screening library of over 1000 proprietary lipid-formulations, enabling efficient identification and optimization of cell line and application specific reagents. With over 25 years of developing transfection reagents our R&D team has developed the most efficient products for DNA and RNA applications.

SEM, 10X Mag.

We used the sample products [NeuralQ™ Basal Medium and GS21™ Neural Supplement] you sent,

and we are really pleased with our cultures.

“

“

NOVEL TRANSFECTION TECHNOLOGY

STEM CELL CULTURE

~ SHEELA KADAPAKKAM | UNIVERSITY OF TEXAS HEALTH SCIENCES CENTER, SAN ANTONIO

We develop and manufacture high quality stem cell culture reagents, including feeder cells, media and supplements for optimal, consistent culturing of pluripotent stem cells. Our products are used by scientists in over 500 research laboratories worldwide.

    NeuralQ /GS21                       Top Compe tor 

A 

B  Maximum Cell Growth and Neurite Maturation - Cryopreserved Primary Rat Cortical Neurons

    4 weeks 

were thawed and cultured in NeuralQ™ Basal Medium (GSM-9420) plus 2% GS21™ Supplement (GSM-3100) (A,A’) or top competitor neuronal basal medium and supplement (B,B’) . Neurons grown in NeuralQ™ with GS21™ show significantly more elaborate neurites after four weeks in

B’ 

A’ 

culture compared to the competitor reagent, and immunofluorescence shows more MAP2

+

dendrites and greater density of Synapsin staining.

We had the opportunity to compare your neuronal medium with to our best. I am surprised (and happy)

“

“

CELL CULTURE MANUFACTURING Utilizing our highly trained personnel and expertise in large-scale primary and stem cell production, we produce over three billion cells each week. We have established a robust infrastructure and standardized quality control procedures to manufacture high quality cell reagents and support custom cell culture projects for our customers.

CUSTOM SOLUTIONS | UNIQUE SERVICES WE OFFER

~ DR. RANDALL COHRS | RESEARCH PROFESSOR , NEUROLOGY - UNIVERSITY OF COLORODO, DENVER

PRODUCTS NeuralQ™ Basal Medium (1X)

DESCRIPTION

CATALOG NO.

NeuralQ™ Basal Medium (1X) is optimized for maximum growth and long-term viability of neural cells in culture.

GSM-9420

The ability to access our transfection, stem cell, neurobiology and cell culture capabilities uniquely enables us to provide custom cell culture solutions to our customers. Our Custom Solutions business includes projects to screen and optimize transfection reagents, establish primary or stem cell cultures, and differentiate neurons from iPSC and neural stem cell lines to support basic research and drug discovery. Contact us for more information.

Unit Size: 500mL

GS21™ is a serum-free neural supplement based on the formulation of NS21 Supplement and designed to improve the overall performance of neurons in culture. Unit Size: 10mL

GSM-3100

“

“

GS21™ Neuronal Supplement (50X)

Utilizing our intellectual property and know-how, and a specialized team of highly skilled and experienced scientists, we are committed to providing the highest quality products to support advancements in neuroscience.

PRIMARY NEURAL CELLS

NEURAL TRANSFECTION

PRIMARY NEURAL CELLS | RAT AND MOUSE NEURONS, NEURAL STEM CELLS & GLIA

NEURAL TRANSFECTION | DNA, mRNA and siRNA TRANSFECTION REAGENTS

Why isolate your own neural cells? GlobalStem offers a wide range of cryopreserved rodent

Developed by the lead members of the scientific team that invented and developed Lipofectamine® and

neural cells. Our proprietary method for cryopreserving cells produces neurons and glial cells with

Lipofectamine® 2000, our specialized transfection reagents are formulations of proprietary compounds that

superior post-thaw viability, offering a convenient tool for your neuroscience applications.

are chemically defined and of animal-free origin. Routinely tested on a wide range of common cell lines our transfection reagents require minimal optimization for superior gene-delivery and expression results.

SUPERIOR TRANSFECTION EFFICIENCY, MAXIMUM CELL VIABILITY

HIGH POST-THAW VIABILITY, FUNCTIONALLY IDENTICAL TO FRESHLY ISOLATED NEURONS

DNA-In™ Neuro Maximum Long-Term Viability - Primary Rat cortical neurons (GSC-8220) remain viable during long- term culture. Above images show cells at 1 week (A), 2 weeks (B) and 3 weeks (C) in culture.

Evoked Synaptic Release - Cryopreserved versus freshly isolated primary rat neurons were each plated at

Superior Transfection Efficiency in Neurons. DNA-In™ Neuro was used to transfect plasmid DNA encoding GFP into 6-day cultured E18 primary rat cortical

80,000 cells per well and grown for 3 weeks. All the cells

neurons. Transfections were performed in 24-well plates using 1.0 - 3.0µl of DNA-In™ Neuro Reagent. Above data shows DNA-In™ Neuro significantly

in the well were stimulated simultaneously. GlobalStem

outperforms Lipofectamine 2000 (LF2K) with a near 3-fold improvement in transfection efficiency after 24 hours (right graph). Duplicate wells were assayed 24

(GS, red trace) cryopreserved neurons exhibit evoked

hours (not shown) and 48 hours after transfection.

synaptic release responses virtually identical to that of freshly isolated rat forebrain neurons (blue trace).

Data courtesy of Pascal Laeng (Galenea Corp., MA).

mRNA-In™ Neuro

Competitor mRNA Reagent Maximum mRNA transfection in neurons - Primary rat cortical neurons were

PRODUCTS Rat Cortical Neurons

DESCRIPTION

CATALOG NO.

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8220 GSC-8240

transfected with 500ng mRNA using mRNA-In™ Neuro or competitor mRNA . Images (left) show significantly high efficiency and expression in cell body and neurites with mRNA-In™ Neuro transfection after 24hrs.

Unit sizes: 2M & 4M viable cells

Isolated from newborn Sprague-Dawley Rats and provided cryopreserved at passage 2.

Rat Astrocytes

Isolated from early embryonic stage (E19) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8040

Unit size: 1M viable cells

GSC-8030

“

The product [DNA-In™ Neuro] worked better than my current reagent.

“

Rat Glial Restricted Precursors

~ NEUROSCIENCE RESEARCHER, DNA-In™ NEURO BETA TESTER

Unit size: 1M viable cells

Rat Neural Stem Cells

Isolated from Sprague-Dawley (E14) rat cortex and provided as cryopreserved at passage 0.

GSC-8010

Unit size: 2M viable cells

Rat Hippocampal Neurons

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8260

PRODUCTS DNA-In™ Neuro Transfection Reagent

Unit size: 1M viable cells

Rat Forebrain Neurons

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

Isolated from E17 C57BL/6 embryonic mouse brain and provided cryopreserved as primary cell suspension. Isolated from E17 C57BL/6 embryonic mouse brain and provided cryopreserved as primary cell suspension. Unit sizes: 1M viable cells

GST-2100 series

mRNA-In™ Neuro Transfection Reagent

Formulated specifically for maximum mRNA delivery in primary and iPSC-derived neurons.

GST-2110 series

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

GSC-8320 GSC-8340

Unit sizes: 1M & 4M viable cells

Mouse Hippocampal Neurons

Formulated specifically for maximum DNA delivery and cell viability in neurons and neural progenitors. Simplified single-tube protocol.

CATALOG NO.

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

GSC-8202

Unit size: 2M viable cells

Mouse Cortical Neurons

DESCRIPTION

GSC-8360

siRNA-In™ Neuro Transfection Reagent

Formulated specifically for maximum gene knock-down in neurons.

GST-2120 series

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

Visit our GlobalStem (www.globalstem.com) and Molecular Transfer, Inc. (www.moleculartransfer.com) websites for a complete listing of transfection reagents we offer for stem cells, hard-to-transfect primary cells, and common cell lines.

HUMAN iPSC-DERIVED NEURAL CELLS HUMAN iPSC-DERIVED PRODUCTS | NEURONS & NEURAL STEM CELLS Our human induced pluripotent stem cell (iPSC) derived neural cell products are extensively tested for quality and lot-to-lot consistency, providing reliable reagents for neurotoxicity, drug screening and gene expression applications.

HUMAN iPSC-DERIVED NEURONS FOR DRUG DISCOVERY

Pluripotent Stem Cells

Neural Stem Cells

Mature Neurons

Neurons for Drug Discovery

HUMAN iPSC-DERIVED NEURAL STEM CELLS DIFFERENTIATE INTO NEURONS

HUMAN iPSC-DERIVED NEURAL STEM CELLS

A

B

C

D

E HUMAN iPSC-DERIVED NEURONS

A

B

C

D

E

Tub3/ Nestin

Map2/Synapsin

GFAP/Nestin

Map2/ChAT

Map2/Vglut1

Human iPSC-derived (HIP™) Neural Stem Cells generate a diverse neuronal population - HIP™ Neural Stem Cells derived from human induced pluripotent stem cells are multipotent. These cells proliferate extensively in culture and produce a virtually pure population of neural stem cells. Shown here, nearly all cells express two widely accepted neural stem cell markers, (A) Nestin is expressed in greater than 99%, and Sox 1 in greater than 97%. Under controlled conditions HIP™ NSCs produce high yields of mature neurons with differentiated cultures containing greater than 90% Tub3-positive neurons (B), including GAD67-expressing GABAergeric neurons (C), ChAT-expressing Cholinergic neurons (D), and Glutamine Synthetase (GLSyn)-expressing Glutamatergic neurons (arrows) (E). HIP™ Neurons begin to show (A) high yields of maturing bipolar Tub3-positive neurons with some remaining elongated nestin-positive progenitors when differentiated for 4 days with GS21™ Neural Supplement (GSM-3100). After five weeks of differentiation, cultures show (B) an abundance of MAP2-positive neurons expressing the synaptic marker PRODUCTS

HIP™ Neural Stem Cells

DESCRIPTION

CATALOG NO.

Human induced pluripotent stem (iPS) cell-derived neural stem cells (NSC). These cells are predisposed to spontaneously differentiate into neurons and can be directed into astrocytes and oligodendrocytes.

GSC-4301

Synapsin, (C) astrocytes marked by GFAP, and (D) ChAT-positive cholinergic neurons.

Unit size: 1M viable cells

HIP™ Neurons

A mixed population of early differentiated neuronal progenitor cells derived from an integration-free human induced pluripotent stem (iPS) cell line. Product includes cells, pre-coat matrix, and GS21™ Neural Supplement (GSM-3100).

GSC-4312

Unit size: 5M viable cells

Dopaminergic Neurons GS-DOPA Kit Human iPSC-derived dopaminergic neural precursors (DNP) in a complete kit. Useful for the study of Parkinson's Disease and other neurological disorders. Mature, functional neurons obtained in 12-14 days. Kit includes cells, growth medium and supplements. Unit size: 1M viable cells

GSK-4401 Human iPSC-Derived Dopaminergic Precursors were differentiated for 12 days using the GS-DOPA Kit of cells, media and supplements. Above image shows neuronal marker Tuj-1 (neuronal type β III tubulin) (green), Dapi (blue) nuclei stain. The dopamine release test (left chart shows functional GS-DOPA neurons), a quantitation of stimulated Dopamine release from cells after 12 days of differentiation.

HUMAN iPSC-DERIVED NEURAL CELLS HUMAN iPSC-DERIVED PRODUCTS | NEURONS & NEURAL STEM CELLS Our human induced pluripotent stem cell (iPSC) derived neural cell products are extensively tested for quality and lot-to-lot consistency, providing reliable reagents for neurotoxicity, drug screening and gene expression applications.

HUMAN iPSC-DERIVED NEURONS FOR DRUG DISCOVERY

Pluripotent Stem Cells

Neural Stem Cells

Mature Neurons

Neurons for Drug Discovery

HUMAN iPSC-DERIVED NEURAL STEM CELLS DIFFERENTIATE INTO NEURONS

HUMAN iPSC-DERIVED NEURAL STEM CELLS

A

B

C

D

E HUMAN iPSC-DERIVED NEURONS

A

B

C

D

E

Tub3/ Nestin

Map2/Synapsin

GFAP/Nestin

Map2/ChAT

Map2/Vglut1

Human iPSC-derived (HIP™) Neural Stem Cells generate a diverse neuronal population - HIP™ Neural Stem Cells derived from human induced pluripotent stem cells are multipotent. These cells proliferate extensively in culture and produce a virtually pure population of neural stem cells. Shown here, nearly all cells express two widely accepted neural stem cell markers, (A) Nestin is expressed in greater than 99%, and Sox 1 in greater than 97%. Under controlled conditions HIP™ NSCs produce high yields of mature neurons with differentiated cultures containing greater than 90% Tub3-positive neurons (B), including GAD67-expressing GABAergeric neurons (C), ChAT-expressing Cholinergic neurons (D), and Glutamine Synthetase (GLSyn)-expressing Glutamatergic neurons (arrows) (E). HIP™ Neurons begin to show (A) high yields of maturing bipolar Tub3-positive neurons with some remaining elongated nestin-positive progenitors when differentiated for 4 days with GS21™ Neural Supplement (GSM-3100). After five weeks of differentiation, cultures show (B) an abundance of MAP2-positive neurons expressing the synaptic marker PRODUCTS

HIP™ Neural Stem Cells

DESCRIPTION

CATALOG NO.

Human induced pluripotent stem (iPS) cell-derived neural stem cells (NSC). These cells are predisposed to spontaneously differentiate into neurons and can be directed into astrocytes and oligodendrocytes.

GSC-4301

Synapsin, (C) astrocytes marked by GFAP, and (D) ChAT-positive cholinergic neurons.

Unit size: 1M viable cells

HIP™ Neurons

A mixed population of early differentiated neuronal progenitor cells derived from an integration-free human induced pluripotent stem (iPS) cell line. Product includes cells, pre-coat matrix, and GS21™ Neural Supplement (GSM-3100).

GSC-4312

Unit size: 5M viable cells

Dopaminergic Neurons GS-DOPA Kit Human iPSC-derived dopaminergic neural precursors (DNP) in a complete kit. Useful for the study of Parkinson's Disease and other neurological disorders. Mature, functional neurons obtained in 12-14 days. Kit includes cells, growth medium and supplements. Unit size: 1M viable cells

GSK-4401 Human iPSC-Derived Dopaminergic Precursors were differentiated for 12 days using the GS-DOPA Kit of cells, media and supplements. Above image shows neuronal marker Tuj-1 (neuronal type β III tubulin) (green), Dapi (blue) nuclei stain. The dopamine release test (left chart shows functional GS-DOPA neurons), a quantitation of stimulated Dopamine release from cells after 12 days of differentiation.

PRIMARY NEURAL CELLS

NEURAL TRANSFECTION

PRIMARY NEURAL CELLS | RAT AND MOUSE NEURONS, NEURAL STEM CELLS & GLIA

NEURAL TRANSFECTION | DNA, mRNA and siRNA TRANSFECTION REAGENTS

Why isolate your own neural cells? GlobalStem offers a wide range of cryopreserved rodent

Developed by the lead members of the scientific team that invented and developed Lipofectamine® and

neural cells. Our proprietary method for cryopreserving cells produces neurons and glial cells with

Lipofectamine® 2000, our specialized transfection reagents are formulations of proprietary compounds that

superior post-thaw viability, offering a convenient tool for your neuroscience applications.

are chemically defined and of animal-free origin. Routinely tested on a wide range of common cell lines our transfection reagents require minimal optimization for superior gene-delivery and expression results.

SUPERIOR TRANSFECTION EFFICIENCY, MAXIMUM CELL VIABILITY

HIGH POST-THAW VIABILITY, FUNCTIONALLY IDENTICAL TO FRESHLY ISOLATED NEURONS

DNA-In™ Neuro Maximum Long-Term Viability - Primary Rat cortical neurons (GSC-8220) remain viable during long- term culture. Above images show cells at 1 week (A), 2 weeks (B) and 3 weeks (C) in culture.

Evoked Synaptic Release - Cryopreserved versus freshly isolated primary rat neurons were each plated at

Superior Transfection Efficiency in Neurons. DNA-In™ Neuro was used to transfect plasmid DNA encoding GFP into 6-day cultured E18 primary rat cortical

80,000 cells per well and grown for 3 weeks. All the cells

neurons. Transfections were performed in 24-well plates using 1.0 - 3.0µl of DNA-In™ Neuro Reagent. Above data shows DNA-In™ Neuro significantly

in the well were stimulated simultaneously. GlobalStem

outperforms Lipofectamine 2000 (LF2K) with a near 3-fold improvement in transfection efficiency after 24 hours (right graph). Duplicate wells were assayed 24

(GS, red trace) cryopreserved neurons exhibit evoked

hours (not shown) and 48 hours after transfection.

synaptic release responses virtually identical to that of freshly isolated rat forebrain neurons (blue trace).

Data courtesy of Pascal Laeng (Galenea Corp., MA).

mRNA-In™ Neuro

Competitor mRNA Reagent Maximum mRNA transfection in neurons - Primary rat cortical neurons were

PRODUCTS Rat Cortical Neurons

DESCRIPTION

CATALOG NO.

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8220 GSC-8240

transfected with 500ng mRNA using mRNA-In™ Neuro or competitor mRNA . Images (left) show significantly high efficiency and expression in cell body and neurites with mRNA-In™ Neuro transfection after 24hrs.

Unit sizes: 2M & 4M viable cells

Isolated from newborn Sprague-Dawley Rats and provided cryopreserved at passage 2.

Rat Astrocytes

Isolated from early embryonic stage (E19) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8040

Unit size: 1M viable cells

GSC-8030

“

The product [DNA-In™ Neuro] worked better than my current reagent.

“

Rat Glial Restricted Precursors

~ NEUROSCIENCE RESEARCHER, DNA-In™ NEURO BETA TESTER

Unit size: 1M viable cells

Rat Neural Stem Cells

Isolated from Sprague-Dawley (E14) rat cortex and provided as cryopreserved at passage 0.

GSC-8010

Unit size: 2M viable cells

Rat Hippocampal Neurons

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

GSC-8260

PRODUCTS DNA-In™ Neuro Transfection Reagent

Unit size: 1M viable cells

Rat Forebrain Neurons

Isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cell suspension.

Isolated from E17 C57BL/6 embryonic mouse brain and provided cryopreserved as primary cell suspension. Isolated from E17 C57BL/6 embryonic mouse brain and provided cryopreserved as primary cell suspension. Unit sizes: 1M viable cells

GST-2100 series

mRNA-In™ Neuro Transfection Reagent

Formulated specifically for maximum mRNA delivery in primary and iPSC-derived neurons.

GST-2110 series

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

GSC-8320 GSC-8340

Unit sizes: 1M & 4M viable cells

Mouse Hippocampal Neurons

Formulated specifically for maximum DNA delivery and cell viability in neurons and neural progenitors. Simplified single-tube protocol.

CATALOG NO.

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

GSC-8202

Unit size: 2M viable cells

Mouse Cortical Neurons

DESCRIPTION

GSC-8360

siRNA-In™ Neuro Transfection Reagent

Formulated specifically for maximum gene knock-down in neurons.

GST-2120 series

Unit sizes: 0.1ml,1 ml, 5x1 ml,10x1ml, Bulk

Visit our GlobalStem (www.globalstem.com) and Molecular Transfer, Inc. (www.moleculartransfer.com) websites for a complete listing of transfection reagents we offer for stem cells, hard-to-transfect primary cells, and common cell lines.

NEURAL CELL CULTURE

ABOUT US COMPANY OVERVIEW | WHO WE ARE

NEURAL CELL CULTURE | NEURAL MEDIA & SUPPLEMENTS Our neural basal medium and supplement are optimized for maximum growth and survival of primary and iPSC-derived neurons in culture. GS21™ Neural Supplement, a serum-free supplement formulation based

Since 2006, GlobalStem, a Molecular Transfer, Inc. company, has been discovering, developing, and commercializing

on the previously published formulation used with NeuralQ™ Basal Medium show superior performance in

innovative technologies and products for cell biology research. Our extensive customer base includes researchers from

supporting overall cell health and viability of neurons during short-term and long-term culture.

major academic, government and industrial institutions.

CORE COMPETENCIES | WHAT WE DO ACHIEVE MAXIMUM NEURON VIABILITY AND CELL GROWTH NEUROBIOLOGY     GS21™ Supplement              Top Compe tor 

Maximum cell viability during short-term and long-term culture Primary Rat Cortical Neurons (GSC-8220) cultured in GS21™

Compe tor 

 

GS21™ 

supplemented culture media showed significantly higher neuron viability after 2 weeks, p=0.032 and 4 weeks, p=0.005 in culture, as compared to cells cultured in top competitor supplement. Neurons

Our neurobiology portfolio includes neural cells, media and supplements to support neurotoxicity, drug screening or gene expression studies. Our proprietary cryopreservation process provides for consistent post-thaw viability of our neural cells. Researchers can confidently standardize their neural cell culture protocols with our high quality basal media and supplements. For your large-scale or technically challenging projects we offer custom services and products for neural cell isolation and differentiation. Contact us for more details.

plated in 96-well plates were labeled with MAP2 (green), and Dapi (blue) to denote nuclei. Data from n=4 wells is shown as mean ±

We have developed a screening library of over 1000 proprietary lipid-formulations, enabling efficient identification and optimization of cell line and application specific reagents. With over 25 years of developing transfection reagents our R&D team has developed the most efficient products for DNA and RNA applications.

SEM, 10X Mag.

We used the sample products [NeuralQ™ Basal Medium and GS21™ Neural Supplement] you sent,

and we are really pleased with our cultures.

“

“

NOVEL TRANSFECTION TECHNOLOGY

STEM CELL CULTURE

~ SHEELA KADAPAKKAM | UNIVERSITY OF TEXAS HEALTH SCIENCES CENTER, SAN ANTONIO

We develop and manufacture high quality stem cell culture reagents, including feeder cells, media and supplements for optimal, consistent culturing of pluripotent stem cells. Our products are used by scientists in over 500 research laboratories worldwide.

    NeuralQ /GS21                       Top Compe tor 

A 

B  Maximum Cell Growth and Neurite Maturation - Cryopreserved Primary Rat Cortical Neurons

    4 weeks 

were thawed and cultured in NeuralQ™ Basal Medium (GSM-9420) plus 2% GS21™ Supplement (GSM-3100) (A,A’) or top competitor neuronal basal medium and supplement (B,B’) . Neurons grown in NeuralQ™ with GS21™ show significantly more elaborate neurites after four weeks in

B’ 

A’ 

culture compared to the competitor reagent, and immunofluorescence shows more MAP2

+

dendrites and greater density of Synapsin staining.

We had the opportunity to compare your neuronal medium with to our best. I am surprised (and happy)

“

“

CELL CULTURE MANUFACTURING Utilizing our highly trained personnel and expertise in large-scale primary and stem cell production, we produce over three billion cells each week. We have established a robust infrastructure and standardized quality control procedures to manufacture high quality cell reagents and support custom cell culture projects for our customers.

CUSTOM SOLUTIONS | UNIQUE SERVICES WE OFFER

~ DR. RANDALL COHRS | RESEARCH PROFESSOR , NEUROLOGY - UNIVERSITY OF COLORODO, DENVER

PRODUCTS NeuralQ™ Basal Medium (1X)

DESCRIPTION

CATALOG NO.

NeuralQ™ Basal Medium (1X) is optimized for maximum growth and long-term viability of neural cells in culture.

GSM-9420

The ability to access our transfection, stem cell, neurobiology and cell culture capabilities uniquely enables us to provide custom cell culture solutions to our customers. Our Custom Solutions business includes projects to screen and optimize transfection reagents, establish primary or stem cell cultures, and differentiate neurons from iPSC and neural stem cell lines to support basic research and drug discovery. Contact us for more information.

Unit Size: 500mL

GS21™ is a serum-free neural supplement based on the formulation of NS21 Supplement and designed to improve the overall performance of neurons in culture. Unit Size: 10mL

GSM-3100

“

“

GS21™ Neuronal Supplement (50X)

Utilizing our intellectual property and know-how, and a specialized team of highly skilled and experienced scientists, we are committed to providing the highest quality products to support advancements in neuroscience.

200 PERRY PARKWAY, SUITE 1 | GAITHERSBURG, MD | 20877 301.545.0238 PHONE | 888.545-0238 TOLL-FREE PHONE | 301.424.1989 FAX WWW.GLOBALSTEM.COM | [email protected] | [email protected]

INNOVATIVE PRODUCTS FOR NEUROSCIENCE RESEARCH

@ 2014 GLOBALSTEM. ALL RIGHTS RESERVED PRODUCTS FOR RESEARCH USE ONLY ● NOT FOR THERAPEUTIC OR DIAGNOSTIC USES ● NOVEMBER 2014 V3.1.0

NEURAL CELLS & MEDIA | STEM CELLS & CULTURE REAGENTS | TRANSFECTION | CUSTOM SERVICES