Physiol. Res. 65: 789-798, 2016

Influence of Endothelin 1 Receptor Blockers and a Nitric Oxide Synthase Inhibitor on Reactive Oxygen Species Formation in Rat Lungs P. KLENIEWSKA1, A. GORĄCA1 1

Experimental and Clinical Physiology, Department of Cardiovascular Physiology, Medical University of Lodz, Poland

Received November 14, 2015 Accepted March 18, 2016 On-line July 15, 2016

Summary

Moreover, generation of reactive oxygen species is mediated by

This study was designated to estimate protective role of ETA and

NOS in the lungs.

ETB receptor antagonist against endothelin 1 (ET-1)-induced oxidative stress in lungs and determine whether these effects are

Key words

mediated by nitric oxide (NO) synthase. Experiments were

Endothelin 1 • Endothelin receptor blockers • L-NAME • Lungs

performed on Wistar rats divided into the following groups: I – saline (0.9 % NaCl); II – ET-1 (3 μg/kg b.w.), III – BQ123

Corresponding author

(1 mg/kg b.w.) + ET-1 (3 μg/kg b.w.), IV – BQ788 (3 mg/kg

P. Kleniewska, Experimental and Clinical Physiology, Department

b.w.) + ET-1 (3 μg/kg b.w.), V – N-nitro-L-arginine methyl ester

of Cardiovascular Physiology,

Medical University of Lodz,

(L-NAME) (5 mg/kg b.w.) + ET-1 (3 μg/kg b.w.). ETA and

Mazowiecka 6/8 Street, PL 92-215 Lodz, Poland. E-mail:

ETB receptor antagonists or L-NAME were administered 30 min

[email protected]

before ET-1 injection. The levels of the following substances were measured in the lungs homogenates: thiobarbituric acid

Introduction

reactive substances (TBARS), hydrogen peroxide (H2O2), reduced glutathione (GSH) and tumor necrosis factor-alpha (TNF-α). The results showed that ET-1 significantly increased TBARS, H2O2 (respectively: p