Vol 5 Issue 10 Nov 2015

ISSN No : 2230-7850 ORIGINAL ARTICLE

International Multidisciplinary Research Journal

Indian Streams Research Journal

Executive Editor Ashok Yakkaldevi

Editor-in-Chief H.N.Jagtap

Welcome to ISRJ RNI MAHMUL/2011/38595 ISSN No.2230-7850 Indian Streams Research Journal is a multidisciplinary research journal, published monthly in English, Hindi & Marathi Language. All research papers submitted to the journal will be double - blind peer reviewed referred by members of the editorial board.Readers will include investigator in universities, research institutes government and industry with research interest in the general subjects.

International Advisory Board Flávio de São Pedro Filho Federal University of Rondonia, Brazil

Mohammad Hailat Dept. of Mathematical Sciences, University of South Carolina Aiken

Hasan Baktir English Language and Literature Department, Kayseri

Kamani Perera Regional Center For Strategic Studies, Sri Lanka

Abdullah Sabbagh Engineering Studies, Sydney

Ghayoor Abbas Chotana Dept of Chemistry, Lahore University of Management Sciences[PK]

Janaki Sinnasamy Librarian, University of Malaya

Ecaterina Patrascu Spiru Haret University, Bucharest

Romona Mihaila Spiru Haret University, Romania

Loredana Bosca Spiru Haret University, Romania

Delia Serbescu Spiru Haret University, Bucharest, Romania

Fabricio Moraes de Almeida Federal University of Rondonia, Brazil

Anurag Misra DBS College, Kanpur

Anna Maria Constantinovici AL. I. Cuza University, Romania Ilie Pintea, Spiru Haret University, Romania Xiaohua Yang PhD, USA

George - Calin SERITAN Faculty of Philosophy and Socio-Political Sciences Al. I. Cuza University, Iasi

......More

Titus PopPhD, Partium Christian University, Oradea,Romania

Editorial Board Iresh Swami Pratap Vyamktrao Naikwade ASP College Devrukh,Ratnagiri,MS India Ex - VC. Solapur University, Solapur R. R. Patil Head Geology Department Solapur University,Solapur Rama Bhosale Prin. and Jt. Director Higher Education, Panvel Salve R. N. Department of Sociology, Shivaji University,Kolhapur Govind P. Shinde Bharati Vidyapeeth School of Distance Education Center, Navi Mumbai Chakane Sanjay Dnyaneshwar Arts, Science & Commerce College, Indapur, Pune Awadhesh Kumar Shirotriya Secretary,Play India Play,Meerut(U.P.)

N.S. Dhaygude Ex. Prin. Dayanand College, Solapur Narendra Kadu Jt. Director Higher Education, Pune K. M. Bhandarkar Praful Patel College of Education, Gondia Sonal Singh Vikram University, Ujjain

Rajendra Shendge Director, B.C.U.D. Solapur University, Solapur R. R. Yalikar Director Managment Institute, Solapur Umesh Rajderkar Head Humanities & Social Science YCMOU,Nashik S. R. Pandya Head Education Dept. Mumbai University, Mumbai

Alka Darshan Shrivastava G. P. Patankar S. D. M. Degree College, Honavar, Karnataka Shaskiya Snatkottar Mahavidyalaya, Dhar Maj. S. Bakhtiar Choudhary Director,Hyderabad AP India.

Rahul Shriram Sudke Devi Ahilya Vishwavidyalaya, Indore

S.Parvathi Devi Ph.D.-University of Allahabad

S.KANNAN Annamalai University,TN

Sonal Singh, Vikram University, Ujjain

Satish Kumar Kalhotra Maulana Azad National Urdu University

Address:-Ashok Yakkaldevi 258/34, Raviwar Peth, Solapur - 413 005 Maharashtra, India Cell : 9595 359 435, Ph No: 02172372010 Email: [email protected] Website: www.isrj.org

Indian Streams Research Journal ISSN 2230-7850

ABSTRACT: ackground: In breast cancer (BC) overexpression of some oncogenes as Human epidermal growth factor receptor and Topoisomerase IIA has been shown to play an important role in the development and progression of certain aggressive types. In recent years these proteins have become an important biomarkers. Objective: The aim of this study was to detect the expression of Her2 and TopoIIA genes in both metastatic and non-metastatic groups of cancer breast patients. Subject and Methods: The present study was enrolled on 230 subjects divided into 3groups: Group I (control) which included 50 healthy subjects. Group II which included 40 patients of metastatic breast cancer cases Group III which contains 140 patients with nonmetastatic breast. A detailed history taking, thorough physical and clinical examination and 5 ml of blood were collected from each subject and biochemical tests were done. Total RNA was extracted from whole blood using RNA extraction kit and then reverse transcription was done followed by real time PCR. Results: The study showed significant

B

Impact Factor : 3.1560(UIF) Volume - 5 | Issue - 10 | Nov - 2015

correlation between control and non-metastatic cancer groups as regards’ Her2 and TopoIIA expression (p