Increased Immune System Effectiveness

Increased Immune System Effectiveness With two products—4Life Transfer

Samples containing several different

Factor Classic and 4Life Transfer

blends of transfer factors sourced

Factor Plus—that already set new

from cow colostrum and egg yolks

and higher standards in immune

were incubated with the NK cells for

system support—providing boosts in

various time periods, in order to

Natural Killer (NK) cell activity of 103

identify the most potent blend ratio

and 248 percent—how did 4Life

and the optimal activation time of NK

think of trying to improve them? It

cells to provide maximum health

started with a dedication to the long-

benefits. Comparison of times

term pursuit of transfer factor

demonstrated that the activation time

research, which led to the discovery

of 48 hours promoted the greatest

of eggs as another viable source for

NK cell response.

transfer factors. A commitment to remain the leader in transfer factor technology and to provide the highest quality, most effective products for good health further drove 4Life to be even better. And finally, it came from the belief that one of the best ways to improve 4Life Transfer Factor was to broaden the spectrum of support by combining safe and effective sources of transfer factors.

Results of this study conclusively demonstrated the ability of 4Life Transfer Factor Advanced Formula to boost NK cell activity by 283 percent and of 4Life Transfer Factor Plus Advanced Formula to propel NK cell activity to a remarkable 437 percent above normal immune response (a response established as a baseline from this study). They also showed that 4Life Transfer Factor Classic increased NK cell

4Life researchers and scientists

activity by 204 percent when tested

developed the Transfer Factor E-XF

at a 48-hour activation time. Further,

™ proprietary blend in an effort to

results from this scientific experiment

further maximize immune system

showed that NK cells activated with

support using transfer factor

4Life Transfer Factor Plus Advanced

molecules. Transfer factors were

Formula killed 97 percent of

taken from both the cow and chicken

cancerous cells, which exceeded the

sources and combined in hopes of

Interleukin-2 (IL2) activation kill rate

sparking a synergistic–effect which

of 88% during the same time period.

was definitely achieved.

The Russian scientists who

4Life Transfer Factor Advanced

conducted this study found the

Formula and 4Life Transfer Factor

results to be exceptional and

Plus Advanced Formula both feature

requested further information so their

the scientifically proven Transfer

results could be published in

Factor E-XF proprietary blend.

professional journals. One scientist

Developed exclusively by 4Life using

stated: “The 4Life sample [4Life

patented and patent-pending

Transfer Factor Plus Advanced

technology, the E-XF blend calls

Formula] activated NK cell activity

upon the knowledge of two sources,

more than the Interleukin-2 (IL2)

providing an enhanced combined

drug used as the standard. Here, we

effect of transfer factors from both

now refer to your sample as the

cow colostrum and chicken eggs.

Golden Interleukin,” said Dr.

Research shows that the immune

Kisielevsky, Russian Academy of

building effects of this transfer factor

Medical Sciences.

blend are more effective than that of colostrum or egg sources alone.

These outstanding test results are certain to be reflected in human

In an effort to determine how

health through an increase in

powerful the 4Life Transfer Factor

immune effectiveness, leading to

Advanced formulas are, researchers

improved overall health. People

Calvin McCausland, Ph.D and

around the world can be confident in

Emma Oganova, Ph.D, M.D.

the ability of 4Life Transfer Factor

designed a study to test NK cell

Advanced Formula and 4Life

activity. Dr. Anatoli Vorobiev at the

Transfer Factor Plus Advanced

Russian Academy of Medical

Formula to more effectively

Science directed his team in the

modulate immune system function,

independent testing. Using blinded

making it more response “able” and

cytotoxicity testing, cancerous cells

to provide broad-spectrum support

were combined with NK cells from

that is unmatched in any other health

humans and divided into groups of

supplement today.

NK cells activated with transfer factors and groups of unactivated NK cells.

Transfer Factor and It's Clinical Applications Rhinebeck Health Center &

The Center for Progressive Medicine Providing new possibilities in the quest for wellness Transfer Factor and It's Clinical Applications by Steven J. Bock, MD

Reprinted with Permission from the International Journal of Integrative Medicine The immune system is amazingly complex. Fortunately, nature gives neonates a little help. We are realizing the importance of breast- feeding and immune competency. In this increasingly dangerous world, we are being assaulted by pathogen after pathogen. Our immune systems

are faltering. Transfer factor (TF), the premier immune factor in colostrum, can be a vital part of our armamentarium. Transfer factor trains and continually educates the immune system. H.S. Lawrence discovered transfer factor in 1949, when he demonstrated that an immune fraction of a person’s white blood cells was able to transfer immunity in a nonsensitized individual.

Transfer factors are small molecules of 3,500-6,000 kDa molecular weight, consisting of oligoribonucleotides attached to a peptide molecule. In the past, they were derived from dialyzed white blood cells (WBC), but now can be purified from bovine colostrum. They are produced by T-lymphocytes and can transfer the ability to recognize a pathogen to cells that have not been in contact with the pathogen (memory function). They also heighten the immune system’s ability to react (increased reactivity or inducer function) to pathogens. Transfer factor probably produces a trigger for T-cell recognition of antigen. On the other hand, it may act as a gene product that assists in antigen presentation to other Tcells.(1) This inducer fraction of transfer factor links the immune cells with an antigen-binding site, thereby increasing their reactivity to an antigenic stimulus. The suppressor fraction blocks the response of the T-cells,(2) and signals a down regulation of the immune response. This is useful in allergic or autoimmune conditions. Role of TH1, TH2 Before one can understand the usefulness of transfer factor, it is helpful to have an understanding of the TH1 helper/TH2 helper paradigm. T helper Lymphocytes develop along two lines of cell populations. TH1 cells, which modulate cell-

mediated immunity, produce the cytokines: IL-2, IFN-gamma, and TNF-alpha. TH2 cells, which modulate humoral immunity, or antibody production, produce IL-4, IL-5, IL-6, IL-10, and IL-13. Once you’re familiar with the particular TH1/TH2 predominant phenotypes in a patient, you can more easily identify certain constellations of diseases or conditions, and tailor your therapies. Cell-mediated or TH1 helper responses are important in the body’s ability to defend itself against viruses, fungi, parasites, cancer, and intracellular organisms. Cell-mediated immunity can be tested by: 1. Skin tests–delayed hypersensitivity skin testing; 2. Response to non-specific mitogens, such as phytohemagglutinin (PHA), concavalina, or pokeweed mitogens; 3. Response to specific mitogens, such as diptheria, tetanus, or candida; 4. Response to alloantigens—mixed lymphocyte reaction; 5. T-cell subsets;

6. IL-2R; 7. NK cell level; 8. NK cell activity; 9. IL1 assay; and 10. IL2 and interferon gamma, and other cytokines. If one has a TH2-dominated condition, with decreased cellular immunity and heightened humoral immunity, the conditions that tend to prevail are: 1. Allergies 2. Chronic sinusitis 3. Atopic eczema 4. Asthma 5. Systemic autoimmune conditions such as lupus erythematosus and mercury-induced autoimmunity 6. Vaccination-induced state 7. Certain cases of autism 8. Hyperinsulinism 9. Pertussis vaccination 10. Malaria 11. Helminth infection 12. Hepatitis C 13. Chronic giardiasis 14. Hypercortisolism 15. Chronic candidiasis 16. Cancer 17. Viral infections 18. Ulcerative colitis A TH1-dominated picture would include the following medical states: 1. 2. 3. 4. 5. 6.

Diabetes type 1 Multiple sclerosis Rheumatoid arthritis Uveitis Crohn’s disease Hashimoto’s disease

7. Sj๖gren’s syndrome 8. Psoriasis

9. Sarcoidosis 10. Chronic Lyme disease 11. H. pylori infections 12. E. histolytica

Pregnancy is a TH2-dominant state. This is an advantage during pregnancy, since a TH1dominant state, or cell-mediated immune response, would induce rejection of the fetus and placenta.(3) Because it stimulates a TH1 response in most cases, transfer factor should not be used in pregnancy. Certain autoimmune diseases, such as multiple sclerosis and heumatoid arthritis, which are TH1-dominant states, are ameliorated during pregnancy.(4) Transfer Factor in Gynecological Conditions • HPV infections • Chronic vaginitis • Chronic candidiasis • Gynecologic cancer • Genital herpes TH1-dominant states are generally not helped by transfer factor, and could be exacerbated. Many of them, such as rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, are thought to be possibly caused by an infection or reaction to a pathogen. If the TH1 response is an inadequate attempt of the immune system to fight off a microbe, then ransfer factor would augment that process and be effective in certain cases. Clinically, this is seen in certain cases, e.g., Crohn’s disease, mutiple sclerosis, and chronic Lyme disease, where transfer factor helps a TH1-dominant condition. Transfer factor augments cellmediated immunity or pushes a TH2 to a TH1 state. This is useful in TH2-dominated conditions. Normally, on exposure to gut-related microbes and childhood infections, a child’s TH2-dominated immune system is subject to TH1 stimulation and TH1/TH2 balance ensues.(5) If TH2 dominance remains, this can lead to atopic, or allergic states. We see this in the increased incidence of allergic symptoms, postnasal drip, asthma, etc., in clinical practice. The other side of this TH2 state is a decreased TH1 or cell-mediated immunity. With this, we see an increased incidence of viral infection, fungal infection, and cancer.

Vaccinations tend to push the immune system toward a TH2-dominant state. To help overcome this tendency, we can use transfer factor pre- and post-immunization.

Cancer, Cell-mediated Immunity, and Transfer Factor Since cancer can be associated with a TH1-deficient state, use of transfer factor should be considered as part of your therapy of immune augmentation in cancer. Factors that decrease cell-mediated immunity and TH2 dominance are age, cytotoxic cancer treatments, postsurgery stress, metastatic disease, etc.(6) Cell-mediated immunity (CMI) can be a predictor of morbidity and mortality over the age of 60. In patients with liver metastases or colon rectal carcinoma, CMI is predictive of survival.(7) Decrease in cell-mediated immunity, along with an increase in circulating immune complexes, indicates unfavorable prognosis in cancer patients.(8) Studies show that patients who have multiple skin cancers had impaired CMI.(9) In a study of gynecological cancer patients compared to control groups, those on chemotherapy had a decrease in immune perimeters (i.e., refractory decreased cell-mediated immunity), whereas the group ,11) Immunologically deficient cancer patients are susceptible to infection by viral pathogens, such as herpes zoster and cytomegalovirus (CMV). Infection occurs as a result of cytotoxic therapy and deficiency of cell-mediated immunity.(12) TH1-dominant states, characterized by increased amounts of IL-2 and IFN-gamma, are immuno-stimulatory and limit tumor growth. In contrast, TH2-predominant patterns, characterized by IL-4 and IL-10 cytokines, are immuno-inhibitng and stimulate tumor growth. HIV progression to HHV8 infection with Kaposi

sarcoma, ulcerative colitis, progression to colon cancer, and obesity with increased incidence of carcinoma, are all associated with the increased TH2 state (and decreased cell-mediated immunity). Studies suggest that this shift to TH2 dominance precedes the cancerous transformation. As the cancer grows, it becomes increasingly hypoxic. This leads to further suppression of cell-mediated immunity, allowing decreased immune surveillance. Studies show that a TH2 immune response is associated with a proangiogenesis state, which facilitates cancer growth.(13) Transfer factor has been shown to improve cellular immunity in patients with immune defects.(14) Since it augments TH1 or cell-mediated immunity, transfer factor is helpful in these situations. For example, by conveying cell-mediated immunity against bladder and prostate tissue-specific antigen, transfer factor was fficacious in the treatment of stage D3 hormone-unresponsive metastatic prostate cancer. Followup showed increased survival rates in 50 patients, with complete remission in two, possible remission in six, and no progression of metastatic disease in (14,15) Transfer factor was shown to improve survival as an adjunct to resection in non-small cell lung carcinoma.(16) Before transfer factor was derived from colostrum, it was obtained from dialyzed leukocyte extract (DLE). The literature has many citations of DLE of an antigenspecific nature being used for various viral conditions, autoimmune conditions, and certain cancers. It has been found that DLE facilitated immunity to tumor-associated antigen. Fudenburg showed that transfer factor could, from selected donors, increase the cellmediated responses to tumor-associated antigens in human osteogenic sarcoma patients. One of the compromises on our cell-mediated immunity is environmental stress (e.g., chemical or heavy metal pollution). It has been shown that long-term exposure to olychlorinated hydrocarbons suppresses phagocytosis, decreases NK cell activity, and reduces lymphocyte response to mitogens in mice.(17) Alterations in immune dysregulation, with a predominantly TH2 response, occurs with lead and mercury exposure. This leads to impaired cell-mediated immunity, increased incidence of infectious disease or cancer, and can end with an autoimmune disease.(18) Transfer Factor in Allergy and ENT Patients • Chronic sinusitis • Chronic allergies • Postnasal drip • Atopic diseases • Asthma • ENT cancer Transfer Factor in GI Practice • Ulcerative colitis& #8226; Viral hepatitis • Chronic candidiasis • astroenteritis • GI cancer

Viral Infections Currently in medicine, we are seeing increased problems with viral infections, such as otitis media, measles, chronic fatigue, Epstein-Barr virus (EBV), CMV, acquired mmunodeficiency syndrome (AIDS), hepatitis, and West Nile virus. We utilize treatment regimens that range from interferon to azidothymidine (AZT), ribavirin, and relenza. However, even with all the high-tech immune weapons available, we are still losing the battle. In the treatment of viral infections, transfer factor provides a modality that works at a fundamental level. It has been shown to induce interferon in patients with viral nfections.(19) Viral infections tend to have increased TH2 and decreased TH1. This is also seen with fungal infections, parasitic diseases, and cancer. Bacterial infections are associated with decreased TH2-dominant states. By stimulating TH1, transfer factor may be advantageous in the reatment of hepatitis. In hepatitis C, the activation of the TH2 dominance plays a role in the development of chronic hepatitis changes. TH1 stimulation may result in clearance of viral particles and mprovement in the hepatitis.(20,21) Studies show that severe complicated measles has been treated successfully with non-specific transfer factor. Symptoms were ameliorated within 24 hours, without side effects.(22)

One theory claims that one of the mechanisms involved in autistic spectrum disorders is an immune imbalance toward a dominant TH2 pattern, resulting from measles, mumps, and rubella (MMR) vaccination. Currently, a study is ongoing to test the efficacy of transfer factor to act as an immune modulator in this disorder. It is well-known that viruses play an important role in the etiology of acute otitis media (AOM) in children. In a study of AOM, 75% of the children were positive for viruses such as respiratory syncytial virus (RSV), para influenza, and influenza, and 48% had the causal viruses in the middle ear effusion.(23) These viruses probably act as antecedents to the bacterial infections typical of AOM.(24) This could account for the excellent results seen in early treatment and prevention of otitis media using transfer factor. A certain percentage of asthmatics have their symptoms precipitated by respiratory infection, most of these secondary to viral infections. A study conducted with transfer factor and asthmatic patients showed that approximately 50% discontinued their steroid medication and the other half decreased their steroid use. Overall, there was a decrease in hospital admissions. Administration of transfer factor improved cellular immunity. No adverse effects or allergic reactions were observed.(25) In Annals of Allergy, Kahn reported the increased incidence of infection, such as para influenza virus, syncytial virus, adenovirus, etc., as precipitating factors in children who have asthma. It was also found that children with asthma have a propensity toward frequent infection.(26) Twelve of 15 children exhibited defects in T-cell immunity, many of which were not rastic.(27) This should emphasize that functional, suboptimal defects in cell-mediated immune function can be a factor in viral illnesses, as measured with sensitive mmunological testing. Once again, we see that transfer factor can help in conditions with increased susceptibility to viruses, a dominant TH2 (decreased cell-mediated) profile. It was found that women with extended human papilloma virus (HPV) infections have defective protective mechanisms of cell-mediated immunity.(28) A pronounced shift from TH1 to TH2 cytokine pattern is associated with more extensive HPV infection. Increased gynecological problems are found secondary to HPV. The potential of transfer factor in HPV infections needs to be further explored. Chronic Infection The addition of transfer factor can help an impaired immune system that is subject to chronic infections. How many practitioners see this scenario: A child comes down with recurrent bronchitis or tonsillitis, starting shortly after birth, necessitating frequent courses of antibiotics. This can then lead to symptoms of chronic candidiasis. A history of chronic eczema or allergic diathesis can also be found. Immunological or skin testing shows a mild defect in cell-mediated immunity, but no abnormalities. Grohn reported on several similar cases and obtained successful treatment with administration of transfer factor.(29) Here we see that transfer factor is helpful for elevated TH2 states, allergy, chronic candidiasis, and eczema. Transfer factor has ameliorated cases of recurrent, non-bacterial cystitis (NBRC) when treatment with antibiotics and nonsteroidal drugs was unsuccessful, and cell-mediated immunity to herpes simplex and candida was decreased.(30) Various studies show positive results with transfer factor in chronic mucocutaneous candidiasis.(31) In Lyme disease, cytotoxic production of a TH2 phenotype is correlated to resistance, while that of a TH1 phenotype is correlated to susceptibility.(32) This suggests that certain people have an immune glitch that makes their immune system prone to either the TH1 or TH2 pattern, and therefore more susceptible to different diseases. This may be precisely where transfer factor, having immune-modulating effects, can be helpful. For instance, in Lyme patients we usually see a TH1-dominated pattern, but transfer factor works very well for certain subsets of Lyme patients.

Chronic Fatigue Transfer factor has been used in chronic fatigue immune dysfunction syndrome, especially if a viral etiology can be found. It has had varied success, although one may need to use increased dosages. If polyvalent transfer factor is not successful, the use of antigen- (or disease-) specific transfer factor may need to be explored.(33) In elderly patients with cellular immunodeficiency and chronic fatigue syndrome, age-related decrease in recovery occurred after treatment with transfer factor.34 Success with transfer factor in chronic fatigue syndrome secondary to human herpes virus 6 (HHV6), genital or labial herpes, and recurrent ocular herpes has been well-documented.35-37 A study on the effect of transfer factor on the course of multiple sclerosis showed that it retarded the progression of the disease in mild to moderate cases.(38)

The Treatment

Treatment with transfer factor is dose dependent. In viral infections, one usually starts with three capsules three times a day. The dose is then tapered down to one capsule three times a day. That dose is maintained in cases of chronic viruses, chronic herpes infection, chronic fatigue secondary to CMV or EBV, chronic colds, and impaired resistance. If there is any flareup in viral infections, the dose can be increased to three capsules three times a day. Usually, patients report decreased susceptibility to colds, decreased nasal symptoms (for instance, postnasal drip and chronic sinus symptoms). In allergic conditions, an adult starts with two capsules three times a day, increasing to three capsules three times a day if symptoms get worse. Again, the dose is tapered to a maintenance level with amelioration of allergic symptoms. In cases of chronic fatigue syndrome, patients start on three capsules three times a day. One may need to increase the dose depending on the response. Doses of four to five capsules three times daily can be used as an adjunct cancer treatment for patients undergoing hemotherapy and/or radiation therapy, with a resulting decrease in cellular immune unction. Transfer Factor for Immune Disorders • • • • • •

Cancer Autoimmune states, e.g., lupus and ulcerative colitis Chronic fatigue Fibromyalgia Lyme disease (certain subsets) EBV, CMV, HHV6, and other viral infections

Various immune function tests, especially those measuring CMI, can be done to gauge maintenance dosage. One can also perform a cytokine panel, measuring IL-2, IL-4, IFN-U, IL10, etc. An elevated IL-2 and interferon gamma would indicate a TH1-predominant state, while an elevated IL-4 and IL-10 would point to a TH2-dominated state. NK cell activity, which is usually decreased in cases of cancer, is increased secondary to transfer factor administration, and can be periodically measured. In pediatric cases with increased susceptibility to viruses, asthma, allergic chronic sinus symptoms, and chronic candida symptoms, initial dosage is: Under 1 Year: 1/2 capsule a day (200mg of transfer factor per capsule). 1-5 Years: 1/2 capsule a day. 6-12 Years: 1 capsule 2 times a day.

Over 12 Years 1 capsule 3 times a day These are starting doses; the doses can be gradually increased depending on the severity of the case. Occasionally, when a patient starts transfer factor, he or she may experience flu-like symptoms, nausea, or gastrointestinal symptoms. Since transfer factor is a small peptide and does not contain milk protein, allergic reactions are rare. These symptoms are usually classified as Jared Herxheimer mechanisms, and they probably signify a direct reaction of transfer factor on gut or systemic pathogens. If patients are informed of these possible mild adverse reactions, they are more likely to continue treatment. Transfer Factor in Pediatric Practice • Chronic pharyngitis #8226; Eczema • Allergies

• • • • • • •

Asthma Food allergies Autism (certain subsets) Prevention of vaccination-induced TH2 states Chronic infection Thrush and candidiasis Otitis media

Transfer Factor for Immune Disorders • • • • • •

Cancer Autoimmune states: lupus and ulcerative colitis Chronic fatigue Fibromyalgia Lyme disease (certain subsets) EBV, CMV, HHV6, and other viral infections

Transfer Factor and Other Alternative Therapies In complicated immune cases or in adjunct cancer treatment, it is advantageous to add complementary classes of herbs and nutrients to augment the immune-stimulatory effects. These auxiliary factors boost natural killer cell activity, increase phagocytosis, increase maturation of T-cells, enhance general immunity, and trigger the complement cascade, helping cytotoxicity. Compounds that act synergistically with transfer factor include thymic protein factors, Chinese herbs (such as astragalus, cordyceps, shiitake, maitake, and reishi), inositol hexaphosphate, melatonin, 1-3 beta glucan, glutathione, and ssociated antioxidants. Vitamins A, D, and B6 promote the TH2 pattern, while vitamins E, C, and folate support the production of a TH1 response.(39) Vitamin B12 suppresses the TH1 response.(40) In addition, acupuncture has been found to increase the immune perimeters of CMI. Levels of CD 3+, CD 4+, CD 4+/CD8+, and beta-endorphins were found to be increased in patients with malignant tumors after a course of acupuncture treatment.(41) Thymic factors cause maturation of na๏ve T-cells and increase cell-mediated immunity. It is known that transfer factor is more effective in post-thymic cells. Therefore, both thymic factors and transfer factors are recommended for mild thymic primary immunodeficiency. (42,43) A recent study by Dr. D. See showed that transfer factor enhanced natural killer cell cytotoxic activity. The effect of transfer factor was greater than that observed with other

well-known NK cell activity enhancers, such as echinacea, acemannan, 1-3 beta glucan, IP-6, and certain Chinese mushrooms. Colostrum had 1/4 the potency. Other immune parameters, such as T-cell function and test of cellular immunity, were not done in this particular study.(44) Conclusion Immune system functioning is at the heart of the increasing infectious and immunologic disorders seen in clinical practices. Through its unique properties and activities, transfer factor is an extremely useful, relatively risk-free alternative and adjunctive therapy for treatment of cell-mediated or TH1-deficient conditions. Think of its potential use in illnesses such as cancer, chronic fatigue, viral infections, allergies, fungal infections, chronic infections, and autoimmune diseases. -----------------------

Steven J. Bock has been practicing alternative and integrative medicine for over 20 years. He has extensive experience in the integrative treatment of Lyme disease. Dr. Bock is a certified acupuncturist. He is medical director of The Rhinebeck Health Center, The Center for Progressive Medicine and PatientsAmerica.com. He is the author of Natural Relief for Your Child's Asthma and Staying Young the Melatonin Way (New York: Plume Books, 1996).

Reprinted with Permission from the International Journal of Integrative Medicine

References 1. Lawrence HS, Borkowsky W: Transfer factor: current status and future prospects. Biotherapy 9:1-5, 1996. 2. Ibid.

3. Kim S, Lira SM, Dalal MA, Verity MA, Voskuhl RR: Estriol ameliorates autoimmune demyelinating disease. Neurology 4:P1230-1237, 1999.

4. Formby: Immunologic response in pregnancy. Endocrine Metabol Clinic North Am 24:187205, 1995. 5. Bjorksten B: Environment and infant immunity. Proc Nutr Soc 58 (3):729-732, August 1999. 6. Nicolini A, Ferrari P, Spinelli R, Carpi A, Sagripanti A, Amborgi F: Cell-mediated immunity in breast cancer patients. Biomed Pharmacother 50(8):337-343, 1996. 7. Bansal AS, Bruce J, Devine PL, Scells B, Zimmermann PV: Serum cytokines and tumor markers in patients with non-small cell carcinoma of the lung. Dis Markers 13(3):195-199, November 1997. 8. Aziz M, Akhtar S, Malik A: Evaluation of cell-mediated immunity and circulating immune complexes as prognostic indicators in cancer patients. Cancer Detect Prev 22(2):87-99, 1998. 9. Czarnechi D, Zaleberg J, Kulinshaya E, Kaz T: Impaired cell-

mediated immunity of apparently normal patients who had multiple skin cancers. Cancer 76(2):228-231, July 15, 1995. 10. Mallmann P, Krebs D: The effect of immunotherapy with thymopentin on the parameters of cellular immunity and the clinical course of gynecologic tumor patients. (Abstract) Onkologie 12(Suppl 3):15-21, June 1989. 11. Mallmann P, Krebs D: Investigations on cell-mediated immunity in patients with breast and ovarian carcinomas receiving a combination of chemotherapy and immunotherapy with thymopentin. Methods Find Exp Clin Pharmacol 12(5):333-340, June 1990. 12. Kitahara T, Takeaka T, Yoshino M: Infection and immunosuppression in cancer patients. (Abstract) Gan To Kagaku Ryoho 16(4 Pt 2-1):11081114, April 1989. 13. O’Byrne KJ, Dalgleish AG, Browning MJ, Steward WP, Harris AL: The relationship between angiogenesis and the immune response in carcinogenesis and the progression of malignant disease. European Journal of Cancer 36(2000):151-169, September 21, 1999. 14. Lawrence HS: Transfer factor in cellular immunity. The Harvey Lecture Series 68. New York: Academic Press, 1987. 15. Pizza G, De Vinci C, Cuzzocrea D, Menniti D, Aiello E, Maver P, Corrado G, Romagnoli P, Dragoni E, LoConte G, Riolo U, Palareti A, Zucchelli P, Fornarola V, Viza D: A preliminary report on the use of transfer factor for treating stage D3 hormone-unresponsive metastatic prostate cancer. Biotherapy 9(3-1):123-132, 1996. 16. Pilotti V, Mastrotilli M, Pizza G, De Vinci C, Busutti L, Palareti A, Gozzetti G, Cavallari A: Transfer factor as an adjuvant to non-small cell lung cancer (NSCLC) therapy. Biotherapy 9:117-121, 1996. 17. Jirova D, Sperlingova I, Halaskova M, Bendova H, Dabrowska L: Immunotoxic effects of carbon tetrachloride: the effect on morphology and function of the immune system in mice. Cent Eur J Public Health 4 (1):16-20, February 1996. 18. Heo Y, Lee WT, Lawrence DA: In vivo the environmental pollutants lead and mercury induce oligoclonal T cell responses skewed toward type-2 reactivities. Cell Immunol 179(2):185-195, August 1, 1997. 19. Khan A: Transfer factor in viral diseases. The Lancet 1 (8059):328-329, February 11, 1978. 20. Milich DR, Chen MK, Hughes JL, Jones JE: The secreted hepatitis precove antigen can modulate the immune response to the nucleocapsid: a mechanism for persistence. J Immunol 160:2013-2021, 1998. 21. Tsai SL, Liaw TF, Chen MH, Huang LY, Kuo GC: Detection of type-2 like T helper cells in hepatitis C detection: implications for hepatitis C virus chronicity. Hepatology 25:449-458, 1997. 22. Ferrer-Argote VE, Romero-Cabello R, Hernandez-Medoza L, AristaViveros A, Rojo-Medina J, Balseca-Olivera F, Fierro M, GonzalezConstandse R: Successful treatment of severe complicated measles with non-specific transfer factor. In Vivo 8:555-558, 1994.

23. McCormick DP, Lim-Melia E, Sneed K, Baldwin CD, Chonmaitice T: Detection of respiratory viruses in middle ear fluids of children with otitis media infections. Ped Infectious Disease Journal 19 (3):256-258, March 2000. 24. Ramilio O: Role of respiratory viruses in acute otitis media: implications for management of AOM. Ped Infectious Disease 18 (12):1125-1129, December 1999. 25. Khan A, Sellars W, Grater W, Graham M, Pflanzer J, Antonetti A, Bailey J, Hill NO: The usefulness of transfer factor in asthma associated with frequent infections. Annals of Allergy 40(4):229-232, April 1978. 26. Ibid. 27. Khan A: The syndrome of asthma, recurrent viral infections and Tcell immuno-deficiency: investigations and management. Annals of Allergy 43(2):69-72, August 1979. 28. Clerici M, Merola M, Ferrario E, Trabattoni D, Villa ML, Stefanon B, Venzon DJ, Shearer GM, De Palo G, Clerici E: Cytokine production patterns in cervical intraepithelial neoplasis: association with human papillomavirus infection. J Natl Cancer Inst 89(3):245-250, February 5, 1997. 29. Grohn P: Transfer factor in chronic and recurrent respiratory tract infections in children. Acta Paediatr Scand 66:211-217, 1977. 30. De Vinci C, Pizza G, Cuzzocrea D, Menniti D, Aiello E, Maver P, Corrado G, Romagnoli P, Dragoni E, LoConte G, Riolo U, Masi M, Severini G, Fornarola V, Viza D: Use of transfer factor for the treatment of recurrent non-bacterial female cystitis (NBRC): a preliminary report. Biotherapy 9(1-3):133-138, 1996. 31. Masi M, De Vinci C, Baricordi OR: Transfer factor in chronic mucocutaneous candidiasis. Biotherapy 9(1-3):97-103, 1996. 32. Matyniak JE, Reiner SL: T helper phenotype and genetic susceptibility in experimental Lyme disease. Journal Exp Med 181 (3):1251-1254, March 1, 1995. 33. Sherwood Lawrence H, Borkowsky W: Transfer factor: current status and future prospects. Biotherapy 9:1-5, 1996. 34. Hana I, Vrubel J, Pekarek J, Cech K: The influence of age on transfer factor treatment of cellular immunodeficiency, chronic fatigue syndrome and/or chronic viral infections. Biotherapy 9(13):91-95, 1996. 35. Ablashi DV, Levine PH, De Vinci C, Whitman JE Jr, Pizza G, Viza D: Use of anti HHV-6 transfer factor for the treatment of two patients with chronic fatigue syndrome (CFS): two case reports. Biotherapy 9(1-3):81-86, 1996. 36. Pizza G, Viza D, De Vinci C, Palareti A, Cuzzocrea D, Fornarola V, Baricordi R: Orally administered HSV: specific transfer factor

(TF) prevents genital or labial herpes relapses. Biotherapy 9(1-3):6772, 1996. 37. Meduri R, Campos E, Scorolli L, De Vinci C, Pizza G, Viza D: Efficacy of transfer factor in treating patients with recurrent ocular herpes infections. Biotherapy 9(1-3):61-66, 1996. 38. Basten A, Pollard JD, Stewart GJ, Frith JA, McLeod JG, Walsh JC, Garrick R, Van Der Brink CM: Transfer factor in treatment of multiple sclerosis. The Lancet 931-934, November 1980. 39. Long KZ, Santos JI: Vitamins and the regulation of the immune response. Pediatr Infect Dis J 18:283-290, 1999. 40. Ibid. 41. Wu B: Effect of acupuncture on the regulation of cell-mediated immunity in the patients with malignant tumors. (Abstract) Chen Tzu Yen Chiu 20(3):67-71, 1995. 42. Lawrence HS: Immune Regulation in Transfer Factor. New York: Academic Press, 1979, p. 613. 43. Khan A, Sellars WA, Gabela P, Thometz D: Transfer factor, thymosin and E rosettes. NEJM 292:868, 1975. 44. See D, Gurnce K, Leclair M: An in vitro screening study of 196 natural products for toxicity and efficacy. JAMA 2(1), December 1999.

CANCER Cancer

Cancers of all types have long been considered among the most horrible diseases. Science and Medicine have made remarkable progress in the war against cancer, yet, according to a report by the American Cancer Society (ACS) more than 1,200,000 new cases expected to be diagnosed in the year 2000. The Centers for Disease Control agree with the ACS and note that approximately 560,000 will die from cancer in 2000. Traditional cancer therapies typically include chemotherapy and radiation. Although conventional medicine has some success against cancer, many new studies have shown several emerging natural compounds to be effective. One compound that deserves specific mention is transfer factors. Transfer factors are now considered one of the greatest discoveries in immunology. Transfer factors work in the immune system by identifying potential diseases. Once identified, other immune cells can attack and destroy the threat. Today, transfer factors continue to be studied with cancer and also with other harmful infections and bacterium with much promise. Although transfer factors have been shown to be effective in a variety of immune compromising conditions, including cancer, transfer factors combine with other specific immune stimulating compounds have shown to be much more effective than transfer factors alone. These compounds include: beta glucans, inositol hexaphosphate, maitake mushroom, shiitake mushroom, cordyceps, aloe vera mannans, and zinc. See below for details: Transfer factors - are small molecules that contain vital immune programming and instruction to better enable the body to recognize and respond to threats of disease. Fudenberg HH, Pizza G, De Vinci C. “Transfer Factor in Malignancy.” Progress in Drug Research 1994. 42: 401-421.

Cech K, Kofranek V, Barnet K, Pekarek J. “The radioprotective effects of DLE-TF on BM mice.” Recent Advances in Transfer Factor and Dialyzed Leucocyte Extracts. Ed. Fujisawa T, Saskawa S, Iikura Y, Komatsu F, Yamaguchi Y. Tokyo, Japan: Maruzen Co Ltd., 1992. 306-313. Cech K, Kafranek V, Marnet K, Pekarek J. “Radioprotective effects of DLE.”Research and Application of Transfer Factor and DLE. Ed. Huo B-L, Wang R-Z, Zou Z-F. Bejing, China: Xueyuan Press, 1989 306-313. Cech K, Sibl O, Pekarek J, Svejcar J. “The adjuvant therapy of the nasopharyngeal tumor with Transfer Factor.” Research and Application of Transfer Factor and DLE. Ed. Bao-lai H, Ru-zhang W, Zhao-fen Z, Bejing, China: Xueyuan Press 1989 403-10. Cech K, Krystukova O, Pekarek J, “DLE in immunosuppreseed patients.”Recent advances in Transfer Factor and Dialyzable Leucocyte Extracts. Ed. Fujisawa T, Saskawa S, Iikkura Y, Komatsu F, Yamaguchi Y. Tokyo, Japan: Maruzen Co. Ltd., 1992. 315-322. Pilotti, V, Mastrorilli M, Pizza G, De Vinci C, Busutti L, Palareti A, Gozzetti G, Cavallari A. Biotherapy 1996. 9:1-3;117-21. Whyte RI, Schork MA, Sloan H, Orringer MB, Kirsh MM. “Adjuvant treatment using Transfer Factor for bronchogenic carcinoma: Long term follow-up.” Ann Thorac Surg. 1992 53:391-6. Fujisawa T, Yamaguchi Y. “Postoperative Immunostimilation after complete resection improves survival of patients with stage I non-small cell lung carcinoma.” Cancer 1996. 78: 1892-8.

IP-6 - (inositol hexaphosphate or phytic acid) important regulator in vital cellular function; anticancer activity. Shamsuddin Am, Vucenik I, Cole KE. “IP6: A novel anti-cancer agent.” Life Science 1997. 61:4; 343-54. Vucenik I, Yang GY, Shamsuddin AM. “Comparison of pure inositol hecaphosphate and high-bran diet in the prevention of DMBA-induced rat mamary carcinogenisis.” Nutr Cancer 1997. 28:1; 7-13. Shamsuddin AM. “Inositol phosphates have novel anticancer function.” J. Nutritioon (Supplement) 1995. 125:3 725S-732S.

Cordyceps Sinensis - Chinese mushroom containing active phytochemicals that safely increase cellular energy levels, acts as an immunomodultor and increases macrophage activity. Yoshida, J, Takamura S, Yamaguchi N, Ren LJ, Chen H, Koshimura S, Suzuki S. “Antitumor activity of an extract of Cordyseps sinensis (Berk.) Sacc. against murine tumor cell lines.” Japan Journal of Experimental Medicine 1989. 59:4, 157-61.

Chiu JH, Ju CH, Wu LH, Lui, WY, Wu CW, Shiao MS, Hong CY. “Cordyceps sinensis increases the expression of major histocompatibility complex class II antigens on human heatoma cell line HA22T/ VGH cells” Am. J. Chin Med 1998. 26(2):159-70. Kuo YC, Tsai WJ, Shiao MS, Chen CF, Lin CY. “Cordyceps sinensis as an immunomodulatory agent.” Am. J. Chin Me 1996. 24:2; 111-25.

Beta Glucans - anit-tumor activity and provides vital signals to activate natural killer (NK) cells. Ross-GD; Vetvicka-V, Yan J, Xia Y, Vetvickova J. “Therapeutic intervention with complement and beta-glucan in cancer.” Immunopharmacology 1999. 42(1-3): 61-74. Zhang MX, Klein B “Activation, binding, and processing of complement component 3 (C3) by Blastomyces dermatitidis.” Infect Immun. 1997 65(5): 1849-55. Vetvicka V, Thornton BP, Wieman TJ, Ross GD. “Targeting of natural killer cells to mammary carcinoma via naturally occurring tumor cell-bound iC3b and beta-glucan-primed CR3 (CD11b/CD18).” J Immunol 1997. 159(2): 599605. Tamura R, Tanebe K, Kawanishi C, Torii K, Ono T. “Effects of lentinan on abnormal ingestive behaviors induced by tumor necrosis factor.” PhysiolBehav1997. 61(3): 399-410. Lia A, Sundberg B, Aman P, Sandberg AS, Hallmans G, Andersson H. “Substrates available for colonic fermentation from oat, barley and wheat bread diets. A study in ileostomy subjects.” Br J Nutr 1996. 76(6): 797-808. Duan X, Ackerly M, Vivier E, Anderson P. “Evidence for involvement of betaglucan-binding cell surface lectins in human natural killer cell function.” Cell Immunol 1994. 157(2): 393-402. Ooi VE, Liu F. “Immunomodulation and anti-cancer activity of polysaccharideprotein complexes.” Curr Med Chem.2000. 7(7): 715-29.

Mannans- carbohydrate from Aloe vera leaf with proven immune enhancing functions. Zhang L, Tizard IR. “Activation of a mouse macrophage cell line by acemannan: the major carbohydrate fraction from Aloe vera gel.” Immunopharmacology 1996. 35(2): 119-28. Womble D, Helderman, JH. “The impact of acemannan on the generation and function of cytotoxic T-ymphoncytes.” Imuonpharmacol Immunotoxicol 1992. 14 (1-2): 63-77.

Maitake and Shiitake Mushrooms- sources of phytochemicals that have anti-cancer properties.

Kurashige S, Akuzawa Y, Endo F. “Effects of Lentinus edodes, Grifola frondosa and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine..” Immunopharmacol-Immunotoxicol 1997. 19(2): 175-83. Chang R. “Functional Properties of edible mushrooms.” Nutr Rev 1996.. 54(11 Pt 2): S91-3. Kim HS, Kacew S, Lee BM. “In vitro chemopreventive effects of plant polysaccharides (Aloe barbadensis miller, Lentinus edodes, Ganoderma lucidum and Coriolus versicolor).” Carcinogenesis 1999. 20(8): 1637-40. Kurashige S, Akuzawa Y, Endo F. “Effects of Lentinus edodes, Grifola frondosa and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine..” Immunopharmacol Immunotoxicol 1997. 19(2): 175-83. Ooi VE, Liu F. “Immunomodulation and anti-cancer activity of polysaccharideprotein complexes. Curr Med Chem 2000. 7(7): 715-29.

Zinc- mineral that is necessary in white blood cell formation and necessary for effective immune response Watson R.R. “Immunological enhancement by fat-soluble vitamins, minerals, and trace metals: a factor in cancer prevention.” Cancer Detect Prev 1986.. 9(1-2): 67-77. Sempertegui, F., et al. "Effects of short-term zinc supplementation on cellular immunity, respiratory symptoms, and growth of malnourished Equadorian children." Eur J Clin Nutr 1996. 50(1): 42-46.

Success Stories

June 12th of 1997 I was diagnosed with multiple myeloma, which is a cancer of the bone marrow, and was told at that time that I probably wouldn't have over two years to live. So we desperately researched every alternative type thing we could, and . . .I guess I tried just about all of them. I got along pretty good until December of 1999 when some new tumors were found and I wound up taking some more chemo for about four months ending in March of 2000. At that time my immune system was pretty badly run down. I thought I was mending again pretty well, and then here about two months ago I heard about a product from Dr. Duane Townsend, M.D.,containing transfer factors, IP-6, beta glucans, maitake mushrooms, zinc and other ingredients. I go to my oncologist every thirty days and have a check-up and a complete blood work-up. A month before starting that product my hemoglobin was down at about 12, my white blood cells were low, my red blood cells low, and all the other stuff they do. There are eight or ten other things they have on the sheet there that I don’t even know what they’re talking about, but the immune system was pretty well run down. Thirty days later I went back and had another blood work-up and it was just amazing. The hemoglobin was up over 14, white blood cells were peaked up, red blood cells were too, just about everything was way up. I guess I can say it’s the only product that I have ever taken that I can say, “Yes, it helped.” I

have become acquainted with a lot of other cancer patients and I’m telling them all about it. M.D. Montana

Clinical Study Proves Effectiveness of 4Life™ Products

Clinical Study Proves Effectiveness of 4Life™ Products Duane Townsend, M.D. • Gynecologic Oncologist Objective: Our medical practice has been collecting data for the past year for a variety of gynecologic conditions to determine what 4Life™ products could do for our patients. Each trial was observed for at least two months and most are ongoing.

BioGenistein Plus™ andFemPro™ used by 81 women - ages: 42 - 75 Condition: Menopausal symptoms Dose: usually one BioGenistein Plus™ and apply FemPro™ twice daily.

35 women with PMS Results: symptoms controlled in 33 women (4 week time response) 32 women with abnormal uterine bleeding

Results: 45 were asymptomatic

Results: 26 returned to normal (some had results immediately, others saw results by 4 - 6 weeks)

21 had mild hot flashes requiring some estrogen

FibroAMJ™ 57 patients, primarily women ages: 40 - 50

15 required daily estrogen doses (time response varied)

Conditions: Fibromyalgia, arthritis, CFS.

Ages: 2 - 80 years

Other gynecologic problems:

Dose: maintenance - 3 TF daily Illness - triple daily dose

ages: women 25 - 50 years dose: usually one BioGenistein Plus™ and apply FemPro™ twice daily.

dosage/protocol: Special diet, no soft drinks or chocolate, exercise, 8 TF, 3 FibroAMJ™ Day-time and 1 Night-time (if not on prescription sleep medication)

Results: all have noticed dramatic improvement [time response varied according to seriousness of condition (most serious - 4 weeks or more)]

Results:

Transfer Factor™ used with over 300 individuals. Conditions: Cancer, herpes, yeast, condyloma, arthritis, colds, sore throats, and lupus.

7 women with fibroid tumors: in six, the tumors are shrinking (3 - 4 months time response)

Results: 55 have had major improvement (4 - 6 weeks time response) * This data is preliminary

Transfer Factor Study with 20 Cancer Patients

Transfer Factor Study with 20 Cancer Patients Darryl See, M.D. conducted the following studies.

Twenty patients, 12 men and 8 women, were selected for this in vivo study. The average age was 49.3. The twenty individuals were each level 3 or level 4 cancer patients. Each patient was basically sent home by his or her oncologist to die. The average life expectancy was 3.7 months. The protocol was to place each patient on 9 capsules per day of Transfer Factor Plus™. The patients were given a number of other general nutrients*. After eight months, 16 of these individuals were still living and were either in remission, improving or stabilized.

The baseline for natural killer cell function was 6.4. Within 4 weeks the average NK Cell function was increased to 25.7 and in 6 months it increased to 27.6. This represented a 400% increase in NK Cell function. This is an ongoing study. This study has been submitted to a peer reviewed publication. *Antioxidants, Digestive Enzymes, Probiotics and multi-vitamins.

______________________________ The following study was conducted at the Institute of Longevity. The purpose of the study was to examine the synergistic effects of the components of Transfer Factor Plus™. Each component was tested separately and then tested as a whole unit. Together the proprietary blend increased NK Cell functions more than the sum total of all of the ingredients tested separately. This study indicates that the intelligence contained inTransfer Factor™ has an enhancing effect on other nutrients. In vitro study: Ingredient 10 GM /ml NK Function PBMC (Control) 25.6% Zinc 26.8% NS Proprietary Blend 59.9%